logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0087 : Histone H3.2

[ CaMP Format ]

* Basic Information

OrganismBos taurus (cattle)
Protein NamesPREDICTED: histone H3.2 [Bos taurus]; histone H3.2; Histone H3.2
Gene NamesLOC619141; Derived by automated computational analysis using gene prediction method: GNOMON. Supporting evidence includes similarity to: 1 mRNA, 13 Proteins
Gene LocusNot available
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
XP_876553 XM_871460 619141 P84227 N/A N/A N/A bta:619141

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 136 aa

Average Mass: 15.388 kDa

Monoisotopic Mass: 15.379 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
--- cleavage 10 ---
Centromere kinetochore component CENP-T histone fold 1. 202815.00.0
--- cleavage 23 (inside Centromere kinetochore component CENP-T histone fold 20..28) ---
--- cleavage 28 (inside Centromere kinetochore component CENP-T histone fold 20..28) ---
--- cleavage 28 ---
--- cleavage 33 ---
--- cleavage 34 ---
--- cleavage 49 ---
Kinetochore component CENP-S 1. 7013013.00.0
--- cleavage 119 (inside Kinetochore component CENP-S 70..130) ---
--- cleavage 129 (inside Kinetochore component CENP-S 70..130) ---

3. Sequence Information

Fasta Sequence: SB0087.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

8 [sites]

Source Reference: [PubMed ID: 3038857] Sakai K, Akanuma H, Imahori K, Kawashima S, A unique specificity of a calcium activated neutral protease indicated in histone hydrolysis. J Biochem. 1987 Apr;101(4):911-8.

Cleavage sites (±10aa)

[Site 1] MARTKQTARK10-STGGKAPRKQ

Lys10 Ser

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Met1Ala2Arg3Thr4Lys5Gln6Thr7Ala8Arg9Lys10
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ser11Thr12Gly13Gly14Lys15Ala16Pro17Arg18Lys19Gln20

Sequence conservation (by blast)

[Site 2] GKAPRKQLAT23-KAARKSAPAT

Thr23 Lys

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Gly14Lys15Ala16Pro17Arg18Lys19Gln20Leu21Ala22Thr23
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Lys24Ala25Ala26Arg27Lys28Ser29Ala30Pro31Ala32Thr33

Sequence conservation (by blast)

[Site 3] KQLATKAARK28-SAPATGGVKK

Lys28 Ser

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Lys19Gln20Leu21Ala22Thr23Lys24Ala25Ala26Arg27Lys28
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ser29Ala30Pro31Ala32Thr33Gly34Gly35Val36Lys37Lys38

Sequence conservation (by blast)

[Site 4] KAARKSAPAT33-GGVKKPHRYR

Thr33 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Lys24Ala25Ala26Arg27Lys28Ser29Ala30Pro31Ala32Thr33
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gly34Gly35Val36Lys37Lys38Pro39His40Arg41Tyr42Arg43

Sequence conservation (by blast)

[Site 5] AARKSAPATG34-GVKKPHRYRP

Gly34 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ala25Ala26Arg27Lys28Ser29Ala30Pro31Ala32Thr33Gly34
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gly35Val36Lys37Lys38Pro39His40Arg41Tyr42Arg43Pro44

Sequence conservation (by blast)

[Site 6] HRYRPGTVAL49-REIRRYQKST

Leu49 Arg

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
His40Arg41Tyr42Arg43Pro44Gly45Thr46Val47Ala48Leu49
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Arg50Glu51Ile52Arg53Arg54Tyr55Gln56Lys57Ser58Thr59

Sequence conservation (by blast)

[Site 7] LCAIHAKRVT119-IMPKDIQLAR

Thr119 Ile

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Leu110Cys111Ala112Ile113His114Ala115Lys116Arg117Val118Thr119
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ile120Met121Pro122Lys123Asp124Ile125Gln126Leu127Ala128Arg129

Sequence conservation (by blast)

[Site 8] IMPKDIQLAR129-RIRGERA

Arg129 Arg

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ile120Met121Pro122Lys123Asp124Ile125Gln126Leu127Ala128Arg129
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Arg130Ile131Arg132Gly133Glu134Arg135Ala136---

Sequence conservation (by blast)

* References

None.