CaMPDB :: Substrate

SB0088 : Crystallin, [beta]B1

* Basic Information

Organism	Rattus norvegicus (Norway rat)
Protein Names	crystallin, beta B1 [Rattus norvegicus]; crystallin, beta B1; Beta-crystallin B1; Beta-B1 crystallin; Beta-crystallin B1B
Gene Names	Crybb1; crystallin, beta B1
Gene Locus	12; chromosome 12
GO Function	GO:0005212 - structural constituent of eye lens [Evidence IDA]; GO:0005212 - structural constituent of eye lens [Evidence IEA]

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_037068	NM_012936	25421	P02523	N/A	N/A	N/A	rno:25421

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 249 aa

Average Mass: 28.043 kDa

Monoisotopic Mass: 28.026 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
--- cleavage 49 ---
Peptidase inhibitor family I36 1.	57	100	9.0	0.0
Beta/Gamma crystallin 1.	101	118	8.0	0.0
Peptidase inhibitor family I36 2.	146	185	8.0	0.1

3. Sequence Information

Fasta Sequence: SB0088.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 8405363] David LL, Shearer TR, Beta-crystallins insolubilized by calpain II in vitro contain cleavage sites similar to beta-crystallins insolubilized during cataract. FEBS Lett. 1993 Jun 21;324(3):265-70.

Cleavage sites (±10aa)

[Site 1] SVPRPAAKVG⁴⁹-ELPPGSYRLV

Gly⁴⁹ Glu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ser⁴⁰	Val⁴¹	Pro⁴²	Arg⁴³	Pro⁴⁴	Ala⁴⁵	Ala⁴⁶	Lys⁴⁷	Val⁴⁸	Gly⁴⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Glu⁵⁰	Leu⁵¹	Pro⁵²	Pro⁵³	Gly⁵⁴	Ser⁵⁵	Tyr⁵⁶	Arg⁵⁷	Leu⁵⁸	Val⁵⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
GKGTPSTGTAPAPGPTPVPASVPRPAAKVGELPPGSYRLVVFEQENFQGRRVEFSGECLN

Summary

#	organism	max score	hits	top seq
1	N/A	122.00	14	beta-B1-crystallin
2	Rattus norvegicus	122.00	8	crystallin, beta B1
3	Rattus norvegicus	122.00	2	AF286652_1 betaB1-crystallin
4	Mus musculus	89.00	11	crystallin, beta B1
5	Pan troglodytes	88.60	1	PREDICTED: crystallin, beta B1 isoform 2
6	Homo sapiens	85.50	8	crystallin, beta B1
7	synthetic construct	85.50	1	crystallin beta B1
8	Canis familiaris	70.90	1	PREDICTED: similar to crystallin, beta B1
9	Bos taurus	65.10	5	crystallin, beta B1
10	Gallus gallus	53.10	6	crystallin, beta B1
11	Tetraodon nigroviridis	49.30	11	unnamed protein product
12	Xenopus tropicalis	48.50	1	crystallin, beta B1
13	Danio rerio	47.80	15	hypothetical protein LOC560055
14	Xenopus laevis	47.80	3	beta B1 crystallin
15	Cynops pyrrhogaster	45.40	4	newt beta B1-crystallin
16	Dicentrarchus labrax	43.90	1	crystallin beta B1
17	Xenopus laevis	42.40	2	hypothetical protein LOC494645
18	Rana temporaria	42.00	2	CRBA1_RANTE Beta-crystallin A1 gi
19	Rana catesbeiana	41.60	5	CRB32_RANCA Beta-crystallin A3-2 gi
20	Cavia porcellus	40.40	2	beta A3-crystallin
21	Ovis aries	40.40	1	crystallin, beta B3
22	Canis lupus familiaris	39.70	3	crystallin, beta A1
23	Oryctolagus cuniculus	39.70	1	beta A3-crystallin
24	Contains: Beta-crystallin A3, isoform A1, Delta4 form; Beta-crystallin A3, isoform A1, Delta7 form; Beta-crystallin A3, isoform A1, Delta8 form	38.90	1	CRBA1_BOVIN Beta-crystallin A3
25	Oryctolagus cuniculus	38.50	1	beta A1-crystallin
26	Petenia splendida x Cichlasoma synspilum	35.00	1	AAA50959.1
27	Iguana iguana	35.00	1	betaA2-crystallin
28	Pan troglodytes	34.70	1	PREDICTED: crystallin, beta A4
29	Macropus fuliginosus	34.30	2	CRBA2_MACFL Beta-crystallin A2 (Beta-A2 crystalli
30	Paramecium bursaria Chlorella virus 1	33.90	1	hypothetical protein PBCV1_A565R
31	Danio rerio	33.50	2	crystallin, gamma M2c
32	Cyprinus carpio	33.50	1	CRGM2_CYPCA Gamma-crystallin M2 (Gamma-M2) gi
33	Clarias fuscus	32.70	1	CAA57215.1
34	Synechococcus sp. WH 8102	32.00	1	hypothetical protein SYNW1564
35	Azoarcus sp. EbN1	32.00	1	probable zipA related protein, possibly involved
36	human, lens, Peptide, 204 aa	31.20	1	A Chain A, Structure Of Native Human Beta B2 Crys

Top-ranked sequences

organism	matching
N/A	Query 20 GKGTPSTGTAPAPGPTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 20 GKGTPSTGTAPAPGPTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79
Rattus norvegicus	Query 20 GKGTPSTGTAPAPGPTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 20 GKGTPSTGTAPAPGPTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79
Rattus norvegicus	Query 20 GKGTPSTGTAPAPGPTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 20 GKGTPSTGTAPAPGPTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79
Mus musculus	Query 20 GKGTPSTGTAPAPGPTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 \|\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#+\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 21 GKGAPSTGPAPAPGPTPVPASVPRPAAKVG#DLPPGSYRLIVFEQENFQGRRVEFSGECLN 80
Pan troglodytes	Query 20 GKGTPSTGTAPAPGPTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 \|\|\| \| \|\|+\|+\|\| \| \| +\|\| \|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\|\| \| Sbjct 23 GKGAPPAGTSPSPGTTLAPTTVPITTAKVG#ELPPGNYRLVVFELENFQGRRAEFSGECSN 82
Homo sapiens	Query 20 GKGTPSTGTAPAPGPTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 \|\|\| \| \|\|+\|+\|\| \| \| +\|\| +\|\| #\|\|\|\|\|+\|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\|\| \| Sbjct 23 GKGAPPAGTSPSPGTTLAPTTVPITSAKAA#ELPPGNYRLVVFELENFQGRRAEFSGECSN 82
synthetic construct	Query 20 GKGTPSTGTAPAPGPTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 \|\|\| \| \|\|+\|+\|\| \| \| +\|\| +\|\| #\|\|\|\|\|+\|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\|\| \| Sbjct 23 GKGAPPAGTSPSPGTTLAPTTVPITSAKAA#ELPPGNYRLVVFELENFQGRRAEFSGECSN 82
Canis familiaris	Query 44 PAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 \| \| \|#+\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 43 PTTKAG#DLPPGSYKLVVFEQENFQGRRVEFSGECLN 78
Bos taurus	Query 50 ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 54 ELPPGSYKLVVFEQENFQGRRVEFSGECLN 83
Gallus gallus	Query 33 GPTPVPASVPRPAAKV-G#ELPPG-SYRLVVFEQENFQGRRVEFSGECLN 79 \| \| \|+\| \| \| \|#\| \| ++\|+\|+\|\|\|\|\|\|\|\|\|++\|\|+ \|\|\|\| Sbjct 21 APAPAPSSDPTPVTNSKG#EEPSTEAFRIVIFEQENFQGRQMEFTSECLN 69
Tetraodon nigroviridis	Query 20 GKGTPSTGTAPAPGPTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 \| \| \|\| \| +\| \| \| \|#+ \|\|+\|+++\|+\|\|\|\|\|\|\| \|\| \|\|+\| Sbjct 5 AKSASSQGTDAKDKGAPAASS---KATKTG#DPSLGSFRILLFDQENFQGRMVEVQNECMN 61
Xenopus tropicalis	Query 34 PTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 \| \|\|\|++ \| + \|#+ \|\|+++\|+\|\|\|\|\|\|\|\|\| \|\| \|\| \| Sbjct 18 PAPVPSAKP---TRTG#DPMMGSFKIVLFEQENFQGRHVELLNECGN 60
Danio rerio	Query 33 GPTPVPASVPRPAA----KVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 \| \|\|\| \| \| \| #\|+ \|+\|++ \|\|+\|\|\|\|\|\|\| +\| \| \|\|+\| Sbjct 5 GEKSKPASQPDGKAAQTQKKT#EMGMGNYKMYVFDQENFQGRMIEISNECMN 55
Xenopus laevis	Query 22 GTPSTGTAPAPGPTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 + \| \| \| \| \| \|+ +\| + \|#\| \|++++\|+\|\|\|\|\|\|\|\|\| +\| \|\| \| Sbjct 8 ASASHGEKPVPAPFPIA----KPT-RTG#EPLNGTFKIVLFEQENFQGRHMELLNECGN 60
Cynops pyrrhogaster	Query 34 PTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGEC 77 \| \|\| \| \| \| \|#+ \|+\|++++\|\|\|\|\|\|\|\|\| +\| \|\| Sbjct 29 PPSVPISKPN---KAG#DSSVGTYKIIIFEQENFQGRHLELVNEC 69
Dicentrarchus labrax	Query 47 KVG#ELPPGSYRLVVFEQENFQGRRVEFSGECLN 79 \| \|#\| \|\|\|\|+++\|+\|\|\|\|\|\|\| +\| \|\|+\| Sbjct 31 KTG#EPGMGSYRIMMFDQENFQGRMIEVQNECMN 63
Xenopus laevis	Query 54 GSYRLVVFEQENFQGRRVEFSGECLN 79 \|\|+++ \|++\|\|\|\|\|\|+\|+\|\|\| \| \| Sbjct 12 GSWKITVYDQENFQGKRMEFSSSCPN 37
Rana temporaria	Query 51 LPPGSYRLVVFEQENFQGRRVEFSGECLN 79 +\| \| +++ \|++\|\|\|\|\|\|+\|+\|\|+ \| \| Sbjct 9 VPLGPWKITVYDQENFQGKRMEFTSSCAN 37
Rana catesbeiana	Query 51 LPPGSYRLVVFEQENFQGRRVEFSGECLN 79 +\| \| +++ \|++\|\|\|\|\|\|+\|+\|\|+ \| \| Sbjct 26 VPLGPWKITVYDQENFQGKRMEFTSSCAN 54
Cavia porcellus	Query 44 PAAKVG#ELPP-----GSYRLVVFEQENFQGRRVEFSGECLN 79 \| \|+ #+ \| \| +++ +++\|\|\|\|\|\|+\|+\|\|+ \| \| Sbjct 14 PTTKMA#QTNPMPGSLGPWKITIYDQENFQGKRMEFTSSCSN 54
Ovis aries	Query 54 GSYRLVVFEQENFQGRRVEFSGECLN 79 \|\|\|+++\|+\| \|\|\|\|\|+\| \| + \|\| \| Sbjct 22 GSYKVIVYEMENFQGKRCELTAECPN 47
Canis lupus familiaris	Query 44 PAAKVG#ELPP-----GSYRLVVFEQENFQGRRVEFSGECLN 79 \| \|+ #+ \| \| +++ +++\|\|\|\|\|\|+\|+\|\|+ \| \| Sbjct 14 PTTKMA#QTNPMPGSAGPWKITIYDQENFQGKRMEFTSSCPN 54
Oryctolagus cuniculus	Query 44 PAAKVG#ELPP-----GSYRLVVFEQENFQGRRVEFSGECLN 79 \| \|+ #+ \| \| +++ +++\|\|\|\|\|\|+\|+\|\|+ \| \| Sbjct 14 PTTKMA#QTNPMPGSLGPWKITIYDQENFQGKRMEFTSSCPN 54
Contains: Beta-crystallin A3, isoform A1, Delta4 form; Beta-crystallin A3, isoform A1, Delta7 form; Beta-crystallin A3, isoform A1, Delta8 form	Query 44 PAAKVG#ELPP-----GSYRLVVFEQENFQGRRVEFSGECLN 79 \| \|+ #+ \| \| +++ +++\|\|\|\|\|\|+\|+\|\|+ \| \| Sbjct 14 PTTKMA#QTNPMPGSVGPWKITIYDQENFQGKRMEFTSSCPN 54
Oryctolagus cuniculus	Query 54 GSYRLVVFEQENFQGRRVEFSGECLN 79 \| +++ +++\|\|\|\|\|\|+\|+\|\|+ \| \| Sbjct 12 GPWKITIYDQENFQGKRMEFTSSCPN 37
Petenia splendida x Cichlasoma synspilum	Query 52 PPGSYRLVVFEQENFQGRRVEFSGECLN 79 \|\|\|\|+ ++\|+\|\|\|\|\|+ \| +\| \| Sbjct 84 HQGSYRMRIYERENFQGQNHELMNDCDN 111
Iguana iguana	Query 54 GSYRLVVFEQENFQGRRVEFSGEC 77 \| +++ \|+\|+\|\|\|\|\| \| \|\| \|\| Sbjct 3 GQFKITVWEEENFQGERCEFLMEC 26
Pan troglodytes	Query 54 GSYRLVVFEQENFQGRRVEFSGEC 77 \| +++\|\|++++ \|\|\|\|\| \|\|+ \|\| Sbjct 10 GPWKMVVWDEDGFQGRRHEFTAEC 33
Macropus fuliginosus	Query 49 G#ELPPGSY--RLVVFEQENFQGRRVEFSGEC 77 \|# \| \|\| \|\| ++\|+\|\|\|\|\|\|\| \| +\| Sbjct 3 G#TLSQGSSPARLTLWEEENFQGRRCELMSDC 33
Paramecium bursaria Chlorella virus 1	Query 24 PSTGTAPAPGPTPVPASVPRPAA 46 \|+ \|\| \| \|\|\| \|\| \|\|\|\|+\| Sbjct 354 PTPEPAPVPRPTPGPAPVPRPSA 376
Danio rerio	Query 51 LPPGSYRLVVFEQENFQGRRVEFSGECLN 79 + \|\|\|\|\| ++\|+\|\|\| \|+ \| +\| \| Sbjct 85 MHRGSYRLKIYERENFGGQMYELMDDCDN 113
Cyprinus carpio	Query 51 LPPGSYRLVVFEQENFQGRRVEFSGEC 77 + \|\|\|\|+ ++\|+\|\|\| \|+ \| + +\| Sbjct 83 MHRGSYRMRIYERENFMGQMYEMADDC 109
Clarias fuscus	Query 24 PSTGTAPAPGPTPVPASVPRPAAKVG#ELPPGSYRLVVFEQENFQGRRVEFSGEC 77 \| +\| \| + + \| + # \|+\|\|+ ++\|+\|\|\| \|+ +\| \| +\| Sbjct 64 PDYMSAWGWGNNCIRSCRMIPMYR--#----GAYRMKIYERENFLGQMMEISDDC 111
Synechococcus sp. WH 8102	Query 23 TPSTGTAPAPGPTPVPASVPRPAA 46 \|\|+ \|\|\| \|\|\|\|\|++ \| \| + Sbjct 701 TPAPSATPAPEPTPVPSATPTPES 724
Azoarcus sp. EbN1	Query 24 PSTGTAPAPGPTPVPASVPRPAAK--VG#ELPPG 54 \| \| \|+\| \| \|\|\|+\| \|\|\| \|\|# + \|\| Sbjct 61 PWTTEFPSPDANPAPASIPHPAAHGPVG#RVTPG 93
human, lens, Peptide, 204 aa	Query 57 RLVVFEQENFQGRRVEFSGECLN 79 ++++\|\|\|\|\|\|\|\| \| +\| \| \| Sbjct 17 KIIIFEQENFQGHSHELNGPCPN 39

* References

[PubMed ID: 15060019] Kwitek AE, Gullings-Handley J, Yu J, Carlos DC, Orlebeke K, Nie J, Eckert J, Lemke A, Andrae JW, Bromberg S, Pasko D, Chen D, Scheetz TE, Casavant TL, Soares MB, Sheffield VC, Tonellato PJ, Jacob HJ, High-density rat radiation hybrid maps containing over 24,000 SSLPs, genes, and ESTs provide a direct link to the rat genome sequence. Genome Res. 2004 Apr;14(4):750-7.

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