SB0094 : Crystallin, [beta]B1
[ CaMP Format ]
* Basic Information
| Organism | Bos taurus (cattle) |
| Protein Names | put. beta crystallin subunit beta B1 [Bos taurus]; put. beta crystallin subunit beta B1; Beta-crystallin B1; Beta-B1 crystallin |
| Gene Names | CRYBB1 |
| Gene Locus | Not available |
| GO Function | Not available |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 253 aa
Average Mass: 28.143 kDa
Monoisotopic Mass: 28.126 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
| --- cleavage 12 --- |
| --- cleavage 15 --- |
| DEC-1 protein, N-terminal region 1. | 27 | 71 | 117.0 | 13.2 |
| Beta/Gamma crystallin 1. | 105 | 123 | 8.0 | 0.0 |
| Peptidase inhibitor family I36 1. | 149 | 177 | 7.0 | 0.1 |
3. Sequence Information
Fasta Sequence: SB0094.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
2 [sites] cleaved by Calpain 2
Source Reference: [PubMed ID: 9485487] Shih M, Lampi KJ, Shearer TR, David LL, Cleavage of beta crystallins during maturation of bovine lens. Mol Vis. 1998 Feb 27;4:4.
Cleavage sites (±10aa)
[Site 1] QPAAKASATA12-AVNPGPDGKG
Ala12 
Ala
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Gln3 | Pro4 | Ala5 | Ala6 | Lys7 | Ala8 | Ser9 | Ala10 | Thr11 | Ala12 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Ala13 | Val14 | Asn15 | Pro16 | Gly17 | Pro18 | Asp19 | Gly20 | Lys21 | Gly22 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
MSQPAAKASATAAVNPGPDGKGKAGPPPGPAPGSGPAPAPAP
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Bos taurus |
77.40 |
1 |
crystallin, beta B1 |
| 2 |
Rattus norvegicus |
44.30 |
1 |
AF286652_1 betaB1-crystallin |
| 3 |
Mus musculus |
40.00 |
3 |
Crybb1 protein |
| 4 |
Rattus norvegicus |
36.20 |
1 |
crystallin, beta B1 |
| 5 |
Canis familiaris |
35.80 |
1 |
PREDICTED: similar to crystallin, beta B1 |
| 6 |
Homo sapiens |
32.00 |
1 |
crystallin, beta B1 |
| 7 |
synthetic construct |
32.00 |
1 |
crystallin beta B1 |
Top-ranked sequences
| organism | matching |
|---|
| Bos taurus |
Query 1 MSQPAAKASATA#AVNPGPDGKGKAGPPPGPAPGSGPAPAPAP 42
||||||||||||#||||||||||||||||||||||||||||||
Query 1 MSQPAAKASATA#AVNPGPDGKGKAGPPPGPAPGSGPAPAPAP 42
|
| Rattus norvegicus |
Query 7 KASATA#AVNPGPDGKGKAGPPPGPAPGSGPAPAPA 41
||+|| #||||||||||| | | || || | ||
Sbjct 1 KAAATT#AVNPGPDGKGKGTPSTGTAPAPGPTPVPA 35
|
| Mus musculus |
Query 1 MSQPAAKASATA#AVNPGPDGKGKAGP 26
||| ||||||| #||||||||||| |
Sbjct 1 MSQ-AAKASATT#AVNPGPDGKGKGAP 25
|
| Rattus norvegicus |
Query 15 NPGPDGKGKAGPPPGPAPGSGPAPAPA 41
||||||||| | | || || | ||
Sbjct 14 NPGPDGKGKGTPSTGTAPAPGPTPVPA 40
|
| Canis familiaris |
Query 1 MSQPAAKASATA#AVNPGPDGKGKA 24
||| | ||||||#|||||||||||
Sbjct 1 MSQ-AVKASATA#AVNPGPDGKGKG 23
|
| Homo sapiens |
Query 14 VNPGPDGKGKAGPPPG--PAPGSGPAPAPAP 42
|||||| ||| || | |+||+ || |
Sbjct 15 VNPGPDTKGKGAPPAGTSPSPGTTLAPTTVP 45
|
| synthetic construct |
Query 14 VNPGPDGKGKAGPPPG--PAPGSGPAPAPAP 42
|||||| ||| || | |+||+ || |
Sbjct 15 VNPGPDTKGKGAPPAGTSPSPGTTLAPTTVP 45
|
[Site 2] AKASATAAVN15-PGPDGKGKAG
Asn15 Pro
|
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Ala6 | Lys7 | Ala8 | Ser9 | Ala10 | Thr11 | Ala12 | Ala13 | Val14 | Asn15 |
|
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Pro16 | Gly17 | Pro18 | Asp19 | Gly20 | Lys21 | Gly22 | Lys23 | Ala24 | Gly25 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
MSQPAAKASATAAVNPGPDGKGKAGPPPGPAPGSGPAPAPAPAPA
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Bos taurus |
81.60 |
1 |
crystallin, beta B1 |
| 2 |
Rattus norvegicus |
45.80 |
1 |
AF286652_1 betaB1-crystallin |
| 3 |
Mus musculus |
40.00 |
3 |
Crybb1 protein |
| 4 |
Rattus norvegicus |
38.10 |
1 |
crystallin, beta B1 |
| 5 |
Canis familiaris |
35.80 |
1 |
PREDICTED: similar to crystallin, beta B1 |
| 6 |
Homo sapiens |
32.30 |
1 |
crystallin, beta B1 |
| 7 |
synthetic construct |
32.30 |
1 |
crystallin beta B1 |
Top-ranked sequences
| organism | matching |
|---|
| Bos taurus |
Query 1 MSQPAAKASATAAVN#PGPDGKGKAGPPPGPAPGSGPAPAPAPAPA 45
|||||||||||||||#||||||||||||||||||||||||||||||
Query 1 MSQPAAKASATAAVN#PGPDGKGKAGPPPGPAPGSGPAPAPAPAPA 45
|
| Rattus norvegicus |
Query 7 KASATAAVN#PGPDGKGKAGPPPGPAPGSGPAPAPAPAP 44
||+|| |||#|||||||| | | || || | || |
Sbjct 1 KAAATTAVN#PGPDGKGKGTPSTGTAPAPGPTPVPASVP 38
|
| Mus musculus |
Query 1 MSQPAAKASATAAVN#PGPDGKGKAGP 26
||| ||||||| |||#|||||||| |
Sbjct 1 MSQ-AAKASATTAVN#PGPDGKGKGAP 25
|
| Rattus norvegicus |
Query 15 N#PGPDGKGKAGPPPGPAPGSGPAPAPAPAP 44
|#|||||||| | | || || | || |
Sbjct 14 N#PGPDGKGKGTPSTGTAPAPGPTPVPASVP 43
|
| Canis familiaris |
Query 1 MSQPAAKASATAAVN#PGPDGKGKA 24
||| | |||||||||#||||||||
Sbjct 1 MSQ-AVKASATAAVN#PGPDGKGKG 23
|
| Homo sapiens |
Query 14 VN#PGPDGKGKAGPPPGPAPGSGPAPAPAPAP 44
||#|||| ||| || | +| | || |
Sbjct 15 VN#PGPDTKGKGAPPAGTSPSPGTTLAPTTVP 45
|
| synthetic construct |
Query 14 VN#PGPDGKGKAGPPPGPAPGSGPAPAPAPAP 44
||#|||| ||| || | +| | || |
Sbjct 15 VN#PGPDTKGKGAPPAGTSPSPGTTLAPTTVP 45
|
* References
[PubMed ID: 6527379] Quax-Jeuken Y, Janssen C, Quax W, van den Heuvel R, Bloemendal H, Bovine beta-crystallin complementary DNA clones. Alternating proline/alanine sequence of beta B1 subunit originates from a repetitive DNA sequence. J Mol Biol. 1984 Dec 15;180(3):457-72.
