CaMPDB :: Substrate

SB0104 : CAPN2, m-calpain catalytic subunit, mCL

* Basic Information

Organism	Oryctolagus cuniculus (rabbit)
Protein Names	calpain-2 catalytic subunit [Oryctolagus cuniculus]; calpain-2 catalytic subunit; M-calpain; calpain M-type; millimolar-calpain; calpain-2 large subunit; calcium-activated neutral proteinase 2
Gene Names	CAPN2; calpain 2, (m/II) large subunit
Gene Locus	16; chromosome 16
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_001182651	NM_001195722	100009092	N/A	N/A	N/A	N/A	N/A

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 700 aa

Average Mass: 79.824 kDa

Monoisotopic Mass: 79.775 kDa

2. Domain Information

Annotated Domains: Not Available.

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
--- cleavage 19 ---
OST-HTH/LOTUS domain 1.	307	339	6.0	0.0
Calpain large subunit, domain III 1.	356	506	1.0	0.0
OST-HTH/LOTUS domain 2.	550	563	26.0	0.0
EF hand 1.	583	600	7.0	0.0
EF-hand domain pair 1.	610	621	31.0	0.0
EF hand 2.	657	668	17.0	0.0
OST-HTH/LOTUS domain 3.	672	684	33.0	0.0

3. Sequence Information

Fasta Sequence: SB0104.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 3023314] Imajoh S, Kawasaki H, Suzuki K, Limited autolysis of calcium-activated neutral protease (CANP): reduction of the Ca2+-requirement is due to the NH2-terminal processing of the large subunit. J Biochem. 1986 Sep;100(3):633-42.

Cleavage sites (±10aa)

[Site 1] RDREAAEGLG¹⁹-SHERAVKYLN

Gly¹⁹ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Arg¹⁰	Asp¹¹	Arg¹²	Glu¹³	Ala¹⁴	Ala¹⁵	Glu¹⁶	Gly¹⁷	Leu¹⁸	Gly¹⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser²⁰	His²¹	Glu²²	Arg²³	Ala²⁴	Val²⁵	Lys²⁶	Tyr²⁷	Leu²⁸	Asn²⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAARLARDREAAEGLGSHERAVKYLNQDYEALRDACLEAGTLFQDP

Summary

#	organism	max score	hits	top seq
1	N/A	97.10	12	neutral protease large subunit
2	Homo sapiens	97.10	8	AF261089_1 calpain large polypeptide L2
3	Homo sapiens	97.10	2	calpain 2, large subunit
4	Macaca mulatta	95.50	2	PREDICTED: calpain 2, large subunit
5	Rattus norvegicus	92.40	10	calpain 2
6	Mus musculus	91.70	25	unnamed protein product
7	Gallus gallus	75.90	2	calpain 1, (mu/I) large subunit
8	Coturnix coturnix	75.10	1	quail calpain
9	Xenopus tropicalis	69.30	5	calpain 2, (m/II) large subunit
10	Canis familiaris	68.90	8	PREDICTED: similar to calpain 8
11	Xenopus laevis	67.40	4	calpain 8
12	Stizostedion vitreum vitreum	65.50	1	putative calpain-like protein
13	Xenopus laevis	64.70	2	mu/m-calpain large subunit
14	Tetraodon nigroviridis	63.90	11	unnamed protein product
15	Oncorhynchus mykiss	62.80	1	m-calpain
16	Danio rerio	62.40	6	PREDICTED: similar to Calpain 1, (mu/I) large sub
17	Rattus norvegicus	59.30	1	calpain 1, large subunit
18	synthetic construct	58.90	1	Homo sapiens calpain 1, (mu/I) large subunit
19	Pongo pygmaeus	58.50	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
20	Bos taurus	56.20	6	micromolar calcium activated neutral protease 1
21	Sus scrofa	56.20	2	calpain 1, large subunit
22	Gallus gallus	55.50	1	mu-calpain large subunit
23	Canis familiaris	49.70	1	PREDICTED: similar to Calpain-11 (Calcium-activat
24	Danio rerio	45.10	1	calpain 1, large subunit
25	Oryctolagus cuniculus	37.00	1	calpain 3, (p94)
26	Drosophila melanogaster	33.90	5	calpain
27	Drosophila melanogaster	33.90	3	Calpain-B CG8107-PA
28	Caenorhabditis elegans	32.30	3	CaLPain family member (clp-1)

Top-ranked sequences

organism	matching
N/A	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQDP 49 \|\|\|\|\|\|+\|\|+\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49
Homo sapiens	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQDP 49 \|\|\|\|\|\|+\|\|+\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49
Homo sapiens	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQDP 49 \|\|\|\|\|\|+\|\|+\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49
Macaca mulatta	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQDP 49 \|\|\|\|\|\|+\| +\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLVKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49
Rattus norvegicus	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQDP 49 \|\|\|\|\| +\|\|+\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\|\| \|\|+ \|\|\|\|\| \|\|\|\|\| Sbjct 1 MAGIAMKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49
Mus musculus	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQDP 49 \|\|\|\|\| +\|\|+\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\|\| \|\|+ \|\|\|\|\| \|\|\|\|\| Sbjct 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49
Gallus gallus	Query 2 AGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQDP 49 \|\|\|\|\|\| \|\|\| \|\|\|+\|# \| \|\|\|\|\|\|\|\|\|\|\|\|+ \|+\|+\|\|\|\|+\|\| Sbjct 5 GGIAARLQRDRLRAEGVG#EHNNAVKYLNQDYEALKQECIESGTLFRDP 52
Coturnix coturnix	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQDP 49 \|\|\|\|\|\| \|\|\| \|\|\|+\|# \| \|\|\|\|\|\|\|\|\|\|\|\|+ \|\|+\|+\| \|\|+\|\| Sbjct 4 FGGIAARLQRDRLRAEGVG#EHNNAVKYLNQDYEALKQACIESGALFRDP 52
Xenopus tropicalis	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQD 48 \|+\|+\| \|\|\|++\| \| \|\|\|#++\|+\|+ \|\|+\|\|\|\|\|\|\|+ \|\|++\|\|\|\|+\| Sbjct 1 MSGVADRLAKERALASGLG#TNEKALPYLSQDYEALRNECLQSGTLFKD 48
Canis familiaris	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQDP 49 \|+ +\|\|\|+\|+ + \|\| \|\|\|#\|\|++\|\|\|\|\| \|\|+\| \|\| \|\|+\|\| \|\|+\|\| Sbjct 94 MSALAARIAKQQAAAGGLG#SHQKAVKYLGQDFEKLRQQCLDAGVLFKDP 142
Xenopus laevis	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQD 48 \|+\|\|\|+++\|+\|\| \|+\|\|\|#++++ +\|\|\|\|\|\|\|\| \|+ \|\|\|+ \|\|\|+\| Sbjct 1 MSGIASKVAKDRVLAQGLG#TNDKPLKYLNQDYEQLKARCLESNTLFED 48
Stizostedion vitreum vitreum	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQD 48 \|+\|\|\|\| \| \| \|\| +\|+\|#\| +\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+ +\|\|+\| Sbjct 1 MSGIAATLQRRREKEQGIG#SCSQAVKYLNQDYEALRRRCLESESLFKD 48
Xenopus laevis	Query 2 AGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQDP 49 \|\|\|++\| \|\| \| \|+\|#\|\|+ \|\|\|\| \|\|\|\|\|+\|+ \|+\|+\| \|\|+\|\| Sbjct 5 GGIASKLKIDRLKASGVG#SHDCAVKYQNQDYESLKQQCVESGILFEDP 52
Tetraodon nigroviridis	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQD 48 \|+\|\|\|\|+\| \|+ \|+\| \|#+ +\| \|\|\|\|\|\|+\|+\|\|++\|\|\| \| \|\|\|\| Sbjct 1 MSGIAAKLQHHRDRAQGFG#TASQAQKYLNQDFESLRESCLERGELFQD 48
Oncorhynchus mykiss	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQDP 49 \|+\|+\|+ \|\|+ \| \| \| \|#++ \|\|\|\|\|\|\|\|+\|\|\|\| \|\| \| \|\| \|\| Sbjct 1 MSGMASNLAKKRALAAGFG#TNANAVKYLNQDFEALRAQCLNRGGLFSDP 49
Danio rerio	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQDP 49 + \|++ \|+ ++\| \|\|\|+\|#+++ \|+\|+\|\|\|\|\|\| \|\| \|+\|+\| \|\|+\|\| Sbjct 4 IGGVSTRIYKERLQAEGMG#ANDLAIKFLNQDYEELRRECVESGRLFEDP 52
Rattus norvegicus	Query 3 GIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQD 48 \|++\|++ + \|+ \|\|\|# \|\| \|+\|\|\| \|\|\|\| \|\| \|\|+ \| \|\|\|\| Sbjct 13 GVSAQVQKQRDKELGLG#RHENAIKYLGQDYENLRARCLQNGVLFQD 58
synthetic construct	Query 3 GIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQD 48 \|++\|++ + \| \|\|\|# \|\| \|+\|\|\| \|\|\|\| \|\| \|\|++\|\|\|\|+\| Sbjct 13 GVSAQVQKQRARELGLG#RHENAIKYLGQDYEQLRVRCLQSGTLFRD 58
Pongo pygmaeus	Query 3 GIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQD 48 \|++\|++ + \| \|\|\|# \|\| \|+\|\|\| \|\|\|\| \|\| \|\|++\|\|\|\|+\| Sbjct 13 GVSAQVQKQRARELGLG#RHENAIKYLGQDYEQLRVRCLQSGTLFRD 58
Bos taurus	Query 3 GIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQD 48 \|++\|++ + \| \|\|\|# \|\| \|+\|\|\| \|\|\|\| \|\| \|\|+ \| \|\|+\| Sbjct 13 GVSAQVQKQRAKELGLG#RHENAIKYLGQDYEQLRVHCLQRGALFRD 58
Sus scrofa	Query 3 GIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQD 48 \|++\|++ + \| \|\|\|# \|\| \|+\|\|\| \|\|\|\| \|\| \|\|++\|+\|\|+\| Sbjct 13 GVSAQVQKLRAKELGLG#RHENAIKYLGQDYEQLRAHCLQSGSLFRD 58
Gallus gallus	Query 2 AGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQD 48 \|++\|++ \| \| \| \|\|\|# \|+ \|\|++ \|\|\| \|\|\|\| \|\| +\|+\|\|+\| Sbjct 12 TGVSAQVQRQRAKALGLG#QHQNAVRFRGQDYAALRDDCLRSGSLFRD 58
Canis familiaris	Query 1 MAGIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQD 48 + \|+\|\| ++ \| \|+\|\|\|# \| \| \| \|\|++\| \|+ \|\|\| \| \|\|+\| Sbjct 83 LEGMAAHISHSRLKAKGLG#QHHNAQNYNNQNFEDLQAACLRKGELFED 130
Danio rerio	Query 3 GIAARLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQDP 49 \|+\|\|\|\| + \|\|\|# + \|\|\|+\| \|\|\|\| \|\| ++ \|\|+\|\| Sbjct 8 GMAARLRSQWDRDAGLG#QNHNAVKFLGQDYETLRAQSQQSRRLFEDP 54
Oryctolagus cuniculus	Query 7 RLARDREAAEGLG#SHERAVKYLNQDYEALRDACLEAGTLFQD 48 \|+ \|+\|+ \| \|#+ + +\|\| \|\|+ \|\| \|\| \|\|+\| Sbjct 12 RVIRERDRRNGEG#TVTQPIKYEGQDFVVLRQRCLAQKCLFED 53
Drosophila melanogaster	Query 31 DYEALRDACLEAGTLFQDP 49 \|+++\|\|\|+\|\| \|\|+\|+\|\| Sbjct 245 DFQSLRDSCLANGTMFEDP 263
Drosophila melanogaster	Query 31 DYEALRDACLEAGTLFQDP 49 \|+++\|\|\|+\|\| \|\|+\|+\|\| Sbjct 245 DFQSLRDSCLANGTMFEDP 263
Caenorhabditis elegans	Query 30 QDYEALRDACLEAGTLFQDP 49 \|\|+ \|\|\| \|\|\|+ \|\|+\|\| Sbjct 255 QDFYELRDQCLESKRLFEDP 274

* References

[PubMed ID: 3038855] Minami Y, Emori Y, Kawasaki H, Suzuki K, E-F hand structure-domain of calcium-activated neutral protease (CANP) can bind Ca2+ ions. J Biochem. 1987 Apr;101(4):889-95.

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