CaMPDB :: Substrate

SB0106 : Aggrecan

* Basic Information

Organism	Bos taurus (cattle)
Protein Names	aggrecan core protein precursor [Bos taurus]; aggrecan core protein precursor; aggrecan core protein; aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122); aggrecan epidermal growth factor-like domain-1; CSPCP; cartilage-specific proteoglycan core protein
Gene Names	ACAN; aggrecan
Gene Locus	21; chromosome 21
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_776406	NM_173981	280985	N/A	N/A	N/A	N/A	N/A

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 2327 aa

Average Mass: 242.478 kDa

Monoisotopic Mass: 242.333 kDa

2. Domain Information

Annotated Domains: Not Available.

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
Chordopoxvirus A33R protein 1.	149	183	102.0	0.0
Chordopoxvirus A33R protein 2.	257	283	113.0	0.0
Domain of unknown function 1.	350	403	36.0	0.0
--- cleavage 365 (inside Domain of unknown function 350..403) ---
--- cleavage 474 ---
Chordopoxvirus A33R protein 3.	499	517	119.0	0.0
Chordopoxvirus A33R protein 4.	594	624	116.0	0.0
--- cleavage 719 ---
Immunoglobulin V-set domain 1.	2051	2082	27.0	0.0
Chordopoxvirus A33R protein 5.	2122	2153	106.0	0.0
Sushi repeat (SCR repeat) 1.	2244	2300	1.0	0.6

3. Sequence Information

Fasta Sequence: SB0106.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

3 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 15175011] Oshita H, Sandy JD, Suzuki K, Akaike A, Bai Y, Sasaki T, Shimizu K, Mature bovine articular cartilage contains abundant aggrecan that is C-terminally truncated at Ala719-Ala720, a site which is readily cleaved by m-calpain. Biochem J. 2004 Aug 15;382(Pt 1):253-9.

Cleavage sites (±10aa)

[Site 1] DIPESFFGVG³⁶⁵-GEEDITIQTV

Gly³⁶⁵ Gly

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Asp³⁵⁶	Ile³⁵⁷	Pro³⁵⁸	Glu³⁵⁹	Ser³⁶⁰	Phe³⁶¹	Phe³⁶²	Gly³⁶³	Val³⁶⁴	Gly³⁶⁵

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gly³⁶⁶	Glu³⁶⁷	Glu³⁶⁸	Asp³⁶⁹	Ile³⁷⁰	Thr³⁷¹	Ile³⁷²	Gln³⁷³	Thr³⁷⁴	Val³⁷⁵

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
GYPDPSSRYDAICYTGEDFVDIPESFFGVGGEEDITIQTVTWPDVELPLPRNITEGEARG

Summary

#	organism	max score	hits	top seq
1	N/A	127.00	6	PGCA_BOVIN Aggrecan core protein precursor (Carti
2	Bos taurus	127.00	1	aggrecan 1
3	Canis lupus familiaris	126.00	1	aggrecan 1
4	Rattus norvegicus	125.00	1	aggrecan 1
5	Contains: Aggrecan core protein 2	124.00	1	PGCA_HUMAN Aggrecan core protein precursor (Carti
6	Homo sapiens	123.00	1	Aggrecan
7	Bos taurus	116.00	1	aggrecan interglobular domain
8	Sus scrofa	115.00	2	AF314813_1 aggrecan G2 domain
9	Ovis aries	114.00	1	aggrecan interglobular domain
10	Cavia porcellus	112.00	1	AF095594_1 aggrecan interglobular domain
11	Equus caballus	109.00	1	aggrecan interglobular domain
12	swine, laryngeal cartilage, Peptide Partial, 370 aa	108.00	1	aggrecan {hyaluronan-binding region}
13	Tupaia belangeri	90.10	1	AF143426_1 aggrecan
14	Sus scrofa	87.00	1	aggrecan
15	Gallus gallus	72.80	2	PGCA_CHICK Aggrecan core protein precursor (Carti
16	Tetraodon nigroviridis	40.40	2	unnamed protein product

Top-ranked sequences

organism	matching
N/A	Query 336 GYPDPSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 336 GYPDPSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395
Bos taurus	Query 336 GYPDPSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 336 GYPDPSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395
Canis lupus familiaris	Query 336 GYPDPSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 336 GYPDPSSRYDAICYTGEDFVDIPENFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395
Rattus norvegicus	Query 336 GYPDPSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 336 GYPDPSSRYDAICYTGEDFVDIPENFFGVG#GEEDITIQTVTWPDLELPLPRNITEGEARG 395
Contains: Aggrecan core protein 2	Query 336 GYPDPSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 336 GYPDPSSRYDAICYTGEDFVDIPENFFGVG#GEEDITVQTVTWPDMELPLPRNITEGEARG 395
Homo sapiens	Query 336 GYPDPSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 336 GYPDPSSRYDAICYTGEDFVDIPENFFGVG#GEEDITVQTVTWPDMELPLPRNITEGEARG 395
Bos taurus	Query 340 PSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 340 PSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395
Sus scrofa	Query 340 PSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 PSSRYDAICYTGEDFVDIPENFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 56
Ovis aries	Query 340 PSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 PSSRYDAICYTGEDFVDIPENFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 56
Cavia porcellus	Query 340 PSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 PSSRYDAICYTGEDFVDIPENFFSVG#GEEDITIQTVTWPDMELPLPRNITEGEARG 56
Equus caballus	Query 340 PSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \| #\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|+\|\|\|\|\|\|\|\|\| Sbjct 1 PSSRYDAICYTGEDFVDIPENFFAVS#GEEDITIQTVTWPDVEMPLPQNITEGEARG 56
swine, laryngeal cartilage, Peptide Partial, 370 aa	Query 336 GYPDPSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPR 386 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 320 GYPDPSSRYDAICYTGEDFVDIPENFFGVG#GEEDITIQTVTWPDVELPLPR 370
Tupaia belangeri	Query 350 TGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395 \|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 TGEDFVDIPENFFGVG#GEGDITIQTVTWPDVELPLPRNITEGEARG 46
Sus scrofa	Query 350 TGEDFVDIPESFFGVG#GEEDITIQTVTWPDVELPLPRNITEGEARG 395 \|\|\|\|\|\|\|\|\| +\|\|\|\|\|#\|\| \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 TGEDFVDIPXNFFGVG#GEXDIXIQTVTWPDVELPLPRNITEGEARG 46
Gallus gallus	Query 336 GYPDPSSRYDAICYTGEDFVDIPESFFG--VG#GE--EDITIQTVTWPDVELPLPRNITEG 391 \|\|\| \|\|\|\|\|\|\|\|\|\|+\|+\|\| + \| \|\|# \| \|\|\|\|\|\| +\|\|\|\|\|\|\|\|+\|\| Sbjct 333 GYPHPSSRYDAICYSGDDFEALVPGLFTDEVG#TELGSAFTIQTVTQTEVELPLPRNVTEE 392 Query 392 EARG 395 \|\|\|\| Query 392 EARG 395
Tetraodon nigroviridis	Query 336 GYPDPSSRYDAICYTGEDFVDIPESFFGVG#GEEDITIQTVTW-PDVELPLPRNITEGEAR 394 \|\|\| \| \| \|\|\| \|\|\| + +++ \| \|\| #\| + +\|+ \|\| \|+\| \|\|\|\|\| Sbjct 265 GYPLPESHYDAFCYT--ESLEVESSGFGED#GGDVLTVTKVTQTPEVFFKTTTTTTEGEAV 322 Query 395 G 395 \| Query 395 G 395

[Site 2] VVQVTLAPGA⁴⁷⁴-AEVPGQPRLP

Ala⁴⁷⁴ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Val⁴⁶⁵	Val⁴⁶⁶	Gln⁴⁶⁷	Val⁴⁶⁸	Thr⁴⁶⁹	Leu⁴⁷⁰	Ala⁴⁷¹	Pro⁴⁷²	Gly⁴⁷³	Ala⁴⁷⁴

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala⁴⁷⁵	Glu⁴⁷⁶	Val⁴⁷⁷	Pro⁴⁷⁸	Gly⁴⁷⁹	Gln⁴⁸⁰	Pro⁴⁸¹	Arg⁴⁸²	Leu⁴⁸³	Pro⁴⁸⁴

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
FPRESTPGLGAPTAFTSEDLVVQVTLAPGAAEVPGQPRLPGGVVFHYRPGSSRYSLTFEE

Summary

#	organism	max score	hits	top seq
1	N/A	121.00	6	PGCA_BOVIN Aggrecan core protein precursor (Carti
2	Bos taurus	121.00	6	aggrecan 1
3	Sus scrofa	110.00	2	AF314813_1 aggrecan G2 domain
4	Rattus norvegicus	97.80	3	aggrecan 1
5	Canis lupus familiaris	95.90	1	aggrecan 1
6	Tupaia belangeri	95.10	1	AF143426_1 aggrecan
7	Sus scrofa	92.80	1	aggrecan
8	Homo sapiens	81.60	9	Aggrecan
9	Contains: Aggrecan core protein 2	81.60	1	PGCA_HUMAN Aggrecan core protein precursor (Carti
10	Bos taurus	53.10	1	aggrecan interglobular domain
11	Ovis aries	52.00	1	aggrecan interglobular domain
12	Equus caballus	41.20	1	aggrecan interglobular domain
13	Tetraodon nigroviridis	34.70	4	unnamed protein product
14	Segment 1 of 2	34.70	1	PREDICTED: similar to
15	Canis familiaris	34.30	1	PREDICTED: similar to chondroitin sulfate proteog
16	Gallus gallus	33.90	4	chondroitin sulfate proteoglycan 3
17	Danio rerio	33.50	1	versican b
18	Mus musculus	32.70	11	PG-M/versican
19	Canis familiaris	32.70	1	PREDICTED: similar to Neurocan core protein precu
20	Pan troglodytes	32.30	1	chondroitin sulfate proteoglycan 3
21	Oryctolagus cuniculus	31.20	1	large aggregating cartilage proteoglycan core pro

Top-ranked sequences

organism	matching
N/A	Query 445 FPRESTPGLGAPTAFTSEDLVVQVTLAPGA#AEVPGQPRLPGGVVFHYRPGSSRYSLTFEE 504 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 445 FPRESTPGLGAPTAFTSEDLVVQVTLAPGA#AEVPGQPRLPGGVVFHYRPGSSRYSLTFEE 504
Bos taurus	Query 445 FPRESTPGLGAPTAFTSEDLVVQVTLAPGA#AEVPGQPRLPGGVVFHYRPGSSRYSLTFEE 504 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 445 FPRESTPGLGAPTAFTSEDLVVQVTLAPGA#AEVPGQPRLPGGVVFHYRPGSSRYSLTFEE 504
Sus scrofa	Query 445 FPRESTPGLGAPTAFTSEDLVVQVTLAPGA#AEVPGQPRLPGGVVFHYRPGSSRYSLTFEE 504 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|# \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\| Sbjct 106 FPEESTPGLGAPTAFTSEDLVVQVTMAPGA#TEFPGQPRLPGGVVFHYRQGSSRYSLTFEE 165
Rattus norvegicus	Query 445 FPRESTPGLGAPTAFTSEDLVVQVTLAPGA#AEVPGQPRLPGGVVFHYRPGSSRYSLTFEE 504 \|\| \|+\| \| + \|\|\| \|\|\|\|\|\|+\|\|++\|\|\|# \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\| Sbjct 445 FPEEATRGPDSATAFASEDLVVRVTISPGA#VEVPGQPRLPGGVVFHYRPGSTRYSLTFEE 504
Canis lupus familiaris	Query 446 PRESTPGLGAPTAFTSEDLVVQVTLAPGA#AEVPGQPRLPGGVVFHYRPGSSRYSLTFEE 504 \| \|\|\|\|\| \| \|\|\|\|\|\|\| \|\|\|\|\| \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\| Sbjct 456 PEESTPGP-AFTAFTSEDHVVQVTAVPGA#AEVPGQPRLPGGVVFHYRPGSARYSLTFEE 513
Tupaia belangeri	Query 445 FPRESTPGLGAPTAFTSEDLVVQVTLAPGA#AEVPGQPRLPGGVVFHYRPGSSRYSLT 501 \|\| \| \|\| \|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|#\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|\|\| Sbjct 96 FPEELIPGPGSPTAFTSEDLVVQVTAVPGA#AKVPGQPRLPGGVVFHYRPGPTRYSLT 152
Sus scrofa	Query 445 FPRESTPGLGAPTAFTSEDLVVQVTLAPGA#AEVPGQPRLPGGVVFHYRPGSSRYSLT 501 \|\| \|\|\|\|\|\|\|\|\| \|\|\| \|\|\|\| \|\|+\|\|\|\|# + \|\|\|\| \|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 96 FPXESTPGLGAPXAFTXEDLVXXVTMAPGA#TDFPGQPXLPGXVVFHYRPGSSRYSLT 152
Homo sapiens	Query 450 TPGLGAPTAFTSEDLVVQVTLAPGA#AEVPGQPRLPGGVVFHYRPGSSRYSLTFEE 504 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| # \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|\|\|\|\|\| Sbjct 447 TPGLGPATAFTSEDLVVQVT-----#-AVPGQPHLPGGVVFHYRPGPTRYSLTFEE 495
Contains: Aggrecan core protein 2	Query 450 TPGLGAPTAFTSEDLVVQVTLAPGA#AEVPGQPRLPGGVVFHYRPGSSRYSLTFEE 504 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| # \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|\|\|\|\|\| Sbjct 447 TPGLGPATAFTSEDLVVQVTA----#--VPGQPHLPGGVVFHYRPGPTRYSLTFEE 495
Bos taurus	Query 445 FPRESTPGLGAPTAFTSEDLVVQVTL 470 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 445 FPRESTPGLGAPTAFTSEDLVVQVTL 470
Ovis aries	Query 445 FPRESTPGLGAPTAFTSEDLVVQVTL 470 \|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 106 FPRESTPGVGAPTAFTSEDLVVQVTL 131
Equus caballus	Query 446 PRESTPGLGAPTAFTSEDLVVQVT 469 \| \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 107 PEESTPGLGPITAFTSEDLVVQVT 130
Tetraodon nigroviridis	Query 485 GGVVFHYRPGSSRYSLTFEE 504 \|\|\|\|\|\|\| +\|\|\|+\| ++\| Sbjct 118 NGVVFHYRTNTSRYNLNYQE 137
Segment 1 of 2	Query 486 GVVFHYRPGSSRYSLTFE 503 \|\|\|\|\|\|\| +\|\|\|+\| \|\| Sbjct 149 GVVFHYRAATSRYTLNFE 166
Canis familiaris	Query 486 GVVFHYRPGSSRYSLTFE 503 \|\|\|\|\|\|\| +\|\|\|+\| \|\| Sbjct 610 GVVFHYRAATSRYTLNFE 627
Gallus gallus	Query 486 GVVFHYRPGSSRYSLTF 502 \|\|\|\|\|\|\|\| +\|\|+\|\|\| Sbjct 159 GVVFHYRPAGARYALTF 175
Danio rerio	Query 485 GGVVFHYRPGSSRYSLTF 502 \|\|\|\|\|\|\| +\|\|\|\|\| \| Sbjct 149 SGVVFHYRANTSRYSLNF 166
Mus musculus	Query 486 GVVFHYRPGSSRYSLTF 502 \|\|\|\|\|\|\| +\|\|\|+\| \| Sbjct 149 GVVFHYRAATSRYTLNF 165
Canis familiaris	Query 486 GVVFHYRPGSSRYSLTFEE 504 \|\|\|\|\|\|\| \|\|+\|\|\| \| Sbjct 170 GVVFHYRAAQDRYALTFAE 188
Pan troglodytes	Query 486 GVVFHYRPGSSRYSLTFEE 504 \|\|\|\|\|\|\| \|\|+\|\|\| \| Sbjct 159 GVVFHYRSARDRYALTFAE 177
Oryctolagus cuniculus	Query 486 GVVFHYRPGSSRYSLTFE 503 \|\|\|\|\|\|\| \|+\|\|+\| \|+ Sbjct 95 GVVFHYRAISTRYTLDFD 112

[Site 3] MVTQVGPGVA⁷¹⁹-AVPIGEETTA

Ala⁷¹⁹ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Met⁷¹⁰	Val⁷¹¹	Thr⁷¹²	Gln⁷¹³	Val⁷¹⁴	Gly⁷¹⁵	Pro⁷¹⁶	Gly⁷¹⁷	Val⁷¹⁸	Ala⁷¹⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala⁷²⁰	Val⁷²¹	Pro⁷²²	Ile⁷²³	Gly⁷²⁴	Glu⁷²⁵	Glu⁷²⁶	Thr⁷²⁷	Thr⁷²⁸	Ala⁷²⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
PSPEEEEGSAPTAGPDVEEWMVTQVGPGVAAVPIGEETTAIPGFTVEPENKTEWELAYTP

Summary

#	organism	max score	hits	top seq
1	N/A	119.00	4	PGCA_BOVIN Aggrecan core protein precursor (Carti
2	Bos taurus	119.00	1	aggrecan 1
3	Sus scrofa	84.30	1	AF314813_1 aggrecan G2 domain
4	Canis lupus familiaris	71.20	1	aggrecan 1
5	Rattus norvegicus	63.50	1	aggrecan 1
6	Contains: Aggrecan core protein 2	57.00	1	PGCA_HUMAN Aggrecan core protein precursor (Carti
7	Gallus gallus	37.40	1	PGCA_CHICK Aggrecan core protein precursor (Carti

Top-ranked sequences

organism	matching
N/A	Query 690 PSPEEEEGSAPTAGPDVEEWMVTQVGPGVA#AVPIGEETTAIPGFTVEPENKTEWELAYTP 749 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 690 PSPEEEEGSAPTAGPDVEEWMVTQVGPGVA#AVPIGEETTAIPGFTVEPENKTEWELAYTP 749
Bos taurus	Query 690 PSPEEEEGSAPTAGPDVEEWMVTQVGPGVA#AVPIGEETTAIPGFTVEPENKTEWELAYTP 749 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 690 PSPEEEEGSAPTAGPDVEEWMVTQVGPGVA#AVPIGEETTAIPGFTVEPENKTEWELAYTP 749
Sus scrofa	Query 690 PSPEEEEGSAPTAGPDVEEWMVTQVGPGVA#AVPIGEETTAIPGFTVEPENKTEWELAYTP 749 \|\|\| \|\|\|\| \|\|+ \|\|+\|\| + \|\|\|\|\|\|\|\|#\|\|\|+\|\|\|\|\| + \|\| \|\|\|\|+\|\|\|\| \|\|\| Sbjct 352 PSPGEEEGGTPTSLPDLEERIATQVGPGVA#AVPVGEETTEVAAFTTEPENRTEWEFVYTP 411
Canis lupus familiaris	Query 690 PSPEEEEGSAPTAGPDVEEWMVTQVGPGVA#AVPIGEETTAIPGFTVEPENKTEWELAYTP 749 \|\|\| \|\|\|\| \|\| \|\|+\|+ \|\|\|\|\| \| #+\|\|+\|\|\|\|\|\|\| \|\|+\|\|\|\|+\|\|\|\| \|\|+\| Sbjct 699 PSPGEEEGGTPTPSV-VEDWIPTQVGPVVP#SVPMGEETTAILDFTIEPENQTEWEPAYSP 757
Rattus norvegicus	Query 697 GSAPTAGPDVEEWMVTQVGPGVA#AVPIGEETTAIPGFTVEPENKTEWELAYTP 749 \| \|\|+ \|+\|+\|+\|\|+\| \|\|\| #\|\|\|+ \|\|\| +\| \|\| \|\|\| +\|\|\|\| \|\|\|\| Sbjct 693 GGTPTSPSDIEDWIVTRVEPGVD#AVPLEPETTEVPYFTTEPEKQTEWEPAYTP 745
Contains: Aggrecan core protein 2	Query 690 PSPEEEEGSAPTAGPDVEEWMVTQVGPGVA#AVPIGEETTAIPG--------FTVEPENKT 741 \|\|\| \|\|\|\| \|\|+ \|\|\|\|+\|\|\|\| \|\|\|\|#\|\|\|+ \|\|\|\|\|+\| \|\| \|\|\|\|+\| Sbjct 680 PSPGEEEGGTPTSPSGVEEWIVTQVVPGVA#AVPVEEETTAVPSGETTAILEFTTEPENQT 739 Query 742 EWELAYTP 749 \|\|\| \|\|\|\| Sbjct 740 EWEPAYTP 747
Gallus gallus	Query 707 EEWMVTQVGPGVA#AVPIGEETTAIPGFTVEPENKT 741 \|\| \|\|\|\|+ \|\|\| # +\| \|\|\|\|\| + \|\|\|\|+\| Sbjct 733 EEVMVTQLIPGVE#GIPSGEETTVETELSSEPENQT 767

* References

[PubMed ID: 24032649] Ciechomska M, Wilson CL, Floudas A, Hui W, Rowan AD, van Eden W, Robinson JH, Knight AM, Antigen-specific B lymphocytes acquire proteoglycan aggrecan from cartilage extracellular matrix resulting in antigen presentation and CD4+ T-cell activation. Immunology. 2014 Jan;141(1):70-8. doi: 10.1111/imm.12169.

» More (9) « Hide Below

[PubMed ID: 21289569] ... Desmoulin GT, Hewitt CR, Hunter CJ, Disc strain and resulting positive mRNA expression from application of a noninvasive treatment. Spine (Phila Pa 1976). 2011 Jun 15;36(14):E921-8. doi:

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