CaMPDB :: Substrate

SB0115 : Selenoprotein K

* Basic Information

Organism	Mus musculus (house mouse)
Protein Names	selenoprotein K [Mus musculus]; selenoprotein K; heat shock protein, 30 kDa; Selenoprotein K; SelK
Gene Names	Selk; selenoprotein K
Gene Locus	14; chromosome 14
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_064363	NM_019979	80795	Q9JLJ1	N/A	N/A	N/A	mmu:80795

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 94 aa

Average Mass: 10.642 kDa

Monoisotopic Mass: 10.636 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
Protein of unknown function (DUF2763) 1.	2	91	1.0	0.5
--- cleavage 81 (inside Protein of unknown function (DUF2763) 2..91) ---

3. Sequence Information

Fasta Sequence: SB0115.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites] cleaved by Calpain 1

Source Reference: [PubMed ID: 21849499] Huang Z, Hoffmann FW, Norton RL, Hashimoto AC, Hoffmann PR, Selenoprotein K is a novel target of m-calpain, and cleavage is regulated by Toll-like receptor-induced calpastatin in macrophages. J Biol Chem. 2011 Oct 7;286(40):34830-8. doi: 10.1074/jbc.M111.265520. Epub 2011

Cleavage sites (±10aa)

[Site 1] RRMGRISHLR⁸¹-GPSPPPMAGG

Arg⁸¹ Gly

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Arg⁷²	Arg⁷³	Met⁷⁴	Gly⁷⁵	Arg⁷⁶	Ile⁷⁷	Ser⁷⁸	His⁷⁹	Leu⁸⁰	Arg⁸¹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gly⁸²	Pro⁸³	Ser⁸⁴	Pro⁸⁵	Pro⁸⁶	Pro⁸⁷	Met⁸⁸	Ala⁸⁹	Gly⁹⁰	Gly⁹¹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
YGSSSDSRYDDGRGPPGNPPRRMGRISHLRGPSPPPMAGGUGR

Summary

#	organism	max score	hits	top seq
1	N/A	84.00	4	SELK_MOUSE Selenoprotein K
2	Mus musculus	83.60	3	selenoprotein K
3	Homo sapiens	81.60	2	selenoprotein K
4	Macaca mulatta	79.00	1	PREDICTED: similar to selenoprotein K
5	Rattus norvegicus	78.60	1	heat shock protein
6	Sus scrofa	76.30	1	selenoprotein K
7	Chinchilla lanigera	75.10	1	HSPC030 heat shock-like protein
8	Bos taurus	72.80	1	Selenoprotein K
9	Xenopus laevis	56.20	1	hypothetical protein LOC494630

Top-ranked sequences

organism	matching
N/A	Query 52 YGSSSDSRYDDGRGPPGNPPRRMGRISHLR#GPSPPPMAGGUGR 94 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\| \|\| Sbjct 52 YGSSSDSRYDDGRGPPGNPPRRMGRISHLR#GPSPPPMAGGCGR 94
Mus musculus	Query 52 YGSSSDSRYDDGRGPPGNPPRRMGRISHLR#GPSPPPMAGGUGR 94 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\| Query 52 YGSSSDSRYDDGRGPPGNPPRRMGRISHLR#GPSPPPMAGGUGR 94
Homo sapiens	Query 52 YGSSSDSRYDDGRGPPGNPPRRMGRISHLR#GPSPPPMAGGUGR 94 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 52 YGNSSDSRYDDGRGPPGNPPRRMGRINHLR#GPSPPPMAGGUGR 94
Macaca mulatta	Query 52 YGSSSDSRYDDGRGPPGNPPRRMGRISHLR#GPSPPPMAGG 91 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|#\|\|\|\|\|\|\|\|\|\| Sbjct 52 YGNSSDSRYDDGRGPPGNPPRRMGRINHLR#GPSPPPMAGG 91
Rattus norvegicus	Query 52 YGSSSDSRYDDGRGPPGNPPRRMGRISHLR#GPSPPPMAGG 91 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\| Sbjct 52 YGGSSDSRYDDGRGPPGNPPRRMGRISHLR#GPSPPPMAGG 91
Sus scrofa	Query 52 YGSSSDSRYDDGRGPPGNPPRRMGRISHLR#GPSPPPMAGG 91 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|#\|\|+\|\|\|\|\|\|\| Sbjct 52 YGGSSDSRYDDGRGPPGNPPRRMGRINHLR#GPNPPPMAGG 91
Chinchilla lanigera	Query 52 YGSSSDSRYDDGRGPPGNPPRRMGRISHLR#GPSPPPMAGG 91 \| +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| #\|\|\|\|\|\|\|\|\|\| Sbjct 52 YRNSSDSRYDDGRGPPGNPPRRMGRISHLH#GPSPPPMAGG 91
Bos taurus	Query 52 YGSSSDSRYDDGRGPPGNPP-RRMGRISHLR#GPSPPPMAGG 91 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|+\|\|+#\|\|+\|\|\|\|\|\|\| Sbjct 52 YGSSSDSRYDDGRGPPGNPPRRRMGRINHLQ#GPNPPPMAGG 92
Xenopus laevis	Query 54 SSSDSRYDDGRGPPGNPPRRMGRISHLR#GPSPPPMAGGUG 93 \|\|\| +\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|+\| #\|\|\| \|\|\|\|\|\| \| Sbjct 54 SSSSTRYDDGRGPPGHP-RRMGRINHGS#GPSAPPMAGGGG 92

* References

[PubMed ID: 23444136] Meiler S, Baumer Y, Huang Z, Hoffmann FW, Fredericks GJ, Rose AH, Norton RL, Hoffmann PR, Boisvert WA, Selenoprotein K is required for palmitoylation of CD36 in macrophages: implications in foam cell formation and atherogenesis. J Leukoc Biol. 2013 May;93(5):771-80. doi: 10.1189/jlb.1212647. Epub 2013 Feb 26.

» More (7) « Hide Below

[PubMed ID: 22205701] ... Huang Z, Hoffmann FW, Fay JD, Hashimoto AC, Chapagain ML, Kaufusi PH, Hoffmann PR, Stimulation of unprimed macrophages with immune complexes triggers a low output of nitric oxide by calcium-dependent neuronal nitric-oxide synthase. J Biol Chem. 2012 Feb 10;287(7):4492-502. doi: 10.1074/jbc.M111.315598. Epub 2011

[PubMed ID: 22016385] ... Shchedrina VA, Everley RA, Zhang Y, Gygi SP, Hatfield DL, Gladyshev VN, Selenoprotein K binds multiprotein complexes and is involved in the regulation of endoplasmic reticulum homeostasis. J Biol Chem. 2011 Dec 16;286(50):42937-48. doi: 10.1074/jbc.M111.310920. Epub

[PubMed ID: 21849499] ... Huang Z, Hoffmann FW, Norton RL, Hashimoto AC, Hoffmann PR, Selenoprotein K is a novel target of m-calpain, and cleavage is regulated by Toll-like receptor-induced calpastatin in macrophages. J Biol Chem. 2011 Oct 7;286(40):34830-8. doi: 10.1074/jbc.M111.265520. Epub 2011

[PubMed ID: 21677750] ... Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A, A conditional knockout resource for the genome-wide study of mouse gene function. Nature. 2011 Jun 15;474(7351):337-42. doi: 10.1038/nature10163.

[PubMed ID: 21220695] ... Verma S, Hoffmann FW, Kumar M, Huang Z, Roe K, Nguyen-Wu E, Hashimoto AS, Hoffmann PR, Selenoprotein K knockout mice exhibit deficient calcium flux in immune cells and impaired immune responses. J Immunol. 2011 Feb 15;186(4):2127-37. doi: 10.4049/jimmunol.1002878. Epub 2011

[PubMed ID: 21858002] ... Rederstorff M, Castets P, Arbogast S, Laine J, Vassilopoulos S, Beuvin M, Dubourg O, Vignaud A, Ferry A, Krol A, Allamand V, Guicheney P, Ferreiro A, Lescure A, Increased muscle stress-sensitivity induced by selenoprotein N inactivation in mouse: a mammalian model for SEPN1-related myopathy. PLoS One. 2011;6(8):e23094. doi: 10.1371/journal.pone.0023094. Epub 2011 Aug 8.

[PubMed ID: 12775843] ... Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guigo R, Gladyshev VN, Characterization of mammalian selenoproteomes. Science. 2003 May 30;300(5624):1439-43.