CaMPDB :: Substrate

SB0116 : Vesicular glutamate transporter 2

* Basic Information

Organism	Rattus norvegicus (Norway rat)
Protein Names	vesicular glutamate transporter 2 [Rattus norvegicus]; vesicular glutamate transporter 2; differentiation-associated BNPI; solute carrier family 17 member 6; differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; differentation-associated Na-dependent inorganic phosphate cotransporter; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6; Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6
Gene Names	Slc17a6; Dnpi, Vglut2; Dnpi; Vglut2; solute carrier family 17 (vesicular glutamate transporter), member 6
Gene Locus	1q22; chromosome 1
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_445879	NM_053427	84487	Q9JI12	N/A	N/A	N/A	rno:84487

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 582 aa

Average Mass: 64.575 kDa

Monoisotopic Mass: 64.534 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
BT1 family 1.	88	155	95.0	0.1
BT1 family 2.	220	266	131.0	0.0
Major Facilitator Superfamily 1.	446	513	123.0	0.1

3. Sequence Information

Fasta Sequence: SB0116.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

2 [sites] cleaved by Calpain 1

Source Reference: [PubMed ID: 21807096] Lobo AC, Gomes JR, Catarino T, Mele M, Fernandez P, Inacio AR, Bahr BA, Santos AE, Wieloch T, Carvalho AL, Duarte CB, Cleavage of the vesicular glutamate transporters under excitotoxic conditions. Neurobiol Dis. 2011 Dec;44(3):292-303. doi: 10.1016/j.nbd.2011.07.010. Epub 2011

Cleavage sites (±10aa)

[Site 1] LDEETGDITQ⁵³³-NYINYGTTKS

Gln⁵³³ Asn

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Leu⁵²⁴	Asp⁵²⁵	Glu⁵²⁶	Glu⁵²⁷	Thr⁵²⁸	Gly⁵²⁹	Asp⁵³⁰	Ile⁵³¹	Thr⁵³²	Gln⁵³³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Asn⁵³⁴	Tyr⁵³⁵	Ile⁵³⁶	Asn⁵³⁷	Tyr⁵³⁸	Gly⁵³⁹	Thr⁵⁴⁰	Thr⁵⁴¹	Lys⁵⁴²	Ser⁵⁴³

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
PWADPEETSEEKCGFIHEDELDEETGDITQNYINYGTTKSYGATSQENGGWPNGWEKKEE

Summary

#	organism	max score	hits	top seq
1	Mus musculus	137.00	4	AF324864_1 vesicular glutamate transporter 2
2	Canis familiaris	137.00	2	PREDICTED: similar to transmembrane protein 16E
3	Mus musculus	137.00	2	solute carrier family 17 (sodium-dependent inorga
4	Rattus norvegicus	137.00	2	solute carrier family 17 (sodium-dependent inorga
5	Homo sapiens	132.00	3	solute carrier family 17 (sodium-dependent inorga
6	Bos taurus	126.00	2	solute carrier family 17 (sodium-dependent inorga
7	N/A	95.50	1	VGL2A_DANRE Vesicular glutamate transporter 2.1 (
8	Danio rerio	95.10	3	solute carrier family 17 (sodium-dependent inorga
9	Tetraodon nigroviridis	93.60	5	unnamed protein product
10	Xenopus laevis	90.10	1	glutamate transporter
11	Rattus norvegicus	51.60	2	vesicular glutamate transporter 3
12	Homo sapiens	43.10	1	solute carrier family 17, member 7
13	Sus scrofa	32.00	1	spleen tyrosine kinase

Top-ranked sequences

organism	matching
Mus musculus	Query 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATSQENGGWPNGWEKKEE 563 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATSQENGGWPNGWEKKEE 563
Canis familiaris	Query 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATSQENGGWPNGWEKKEE 563 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1293 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATTQANGGWPNGWEKKEE 1352
Mus musculus	Query 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATSQENGGWPNGWEKKEE 563 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATSQENGGWPNGWEKKEE 563
Rattus norvegicus	Query 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATSQENGGWPNGWEKKEE 563 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATSQENGGWPNGWEKKEE 563
Homo sapiens	Query 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATSQENGGWPNGWEKKEE 563 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|+\|\|\|\|\|\|\| Sbjct 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATTQANGGWPSGWEKKEE 563
Bos taurus	Query 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATSQENGGWPNGWEKKEE 563 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATTQANGGWPNGWEKKEE 563
N/A	Query 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGT-TKSYGATSQENGGWPNGWEKKE 562 \|\|\|\|\|\| \|\|+\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|#+\| \| \|\|\|\|\|\|+\| \|\|\|\| \|\|+\|+\| Sbjct 504 PWADPELTSDEKCGFIDEDELAEETGDITQ#SYGALGAPAKSYGATTQLNGGWAEGWDKRE 563 Query 563 E 563 \| Query 563 E 563
Danio rerio	Query 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGT-TKSYGATSQENGGWPNGWEKKE 562 \|\|\|\|\|\| \|\|+\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|#+\| \| \|\|\|\|\|\|+\| \|\|\|\| \|\|+\|+\| Sbjct 504 PWADPELTSDEKCGFIDEDELAEETGDITQ#SYGALGAPAKSYGATTQLNGGWAEGWDKRE 563 Query 563 E 563 \| Query 563 E 563
Tetraodon nigroviridis	Query 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NY-INYGTTKSYGATSQENGGWPNGWEKKE 562 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|# \| \|\|\|\|\|\|\|\| \|\|\|\| \|+\| \| Sbjct 1537 PWADPEETSEEKCGFIDEDELAEETGDITQ#GYGAMAAPAKSYGATSQLNGGWVQDWDKTE 1596 Query 563 E 563 \| Query 563 E 563
Xenopus laevis	Query 504 PWADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATSQENGGWPNGWEKKEE 563 \|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| +\|+ + \|\|# + \|\|+ + \|\|\|+\|\|\|\|\| \|\|\|\|\|\| Sbjct 496 PWAEPEETSEEKCGFIHEDELADESEEQTQ#AFGGYGSYGATQTTSQQNGGWPAEWEKKEE 555
Rattus norvegicus	Query 505 WADPEETSEEKCGFIHEDELDEETGDITQ#NYINYGTTKSYGATSQ 549 \|\|\|\|\| \|\|\|\|\|\| \| +\|\|\| \|\|\| +# +++ \|\|\|\|\|+\| Sbjct 509 WADPENLSEEKCGIIDQDELAEETELNHE#AFVSPRKKMSYGATTQ 553
Homo sapiens	Query 504 PWADPEETSEEKCGFIHEDEL----DEETGD-ITQ#NYINYGTTKSYGAT 547 \|\|\|+\|\|\| \|\|\|\|\|\|\|+ \|+\| \| \| \| # \|\|\|\|\| Sbjct 496 PWAEPEEMSEEKCGFVGHDQLAGSDDSEMEDEAEP#PGAPPAPPPSYGAT 544
Sus scrofa	Query 514 EKCGFIHEDELDEETGDITQ#NYI---NYGTTKSYGA-----TSQENGGWPNGW 558 \|+\| \|+\| \| +\|\|#+\| ++\| +\|+ \| +\| +\| \|\| \| Sbjct 479 EECNFVHRDLAARNVLLVTQ#HYAKISDFGLSKALRADENYYKAQTHGKWPVKW 531

[Site 2] TQNYINYGTT⁵⁴¹-KSYGATSQEN

Thr⁵⁴¹ Lys

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Thr⁵³²	Gln⁵³³	Asn⁵³⁴	Tyr⁵³⁵	Ile⁵³⁶	Asn⁵³⁷	Tyr⁵³⁸	Gly⁵³⁹	Thr⁵⁴⁰	Thr⁵⁴¹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Lys⁵⁴²	Ser⁵⁴³	Tyr⁵⁴⁴	Gly⁵⁴⁵	Ala⁵⁴⁶	Thr⁵⁴⁷	Ser⁵⁴⁸	Gln⁵⁴⁹	Glu⁵⁵⁰	Asn⁵⁵¹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
SEEKCGFIHEDELDEETGDITQNYINYGTTKSYGATSQENGGWPNGWEKKEEFVQESAQD

Summary

#	organism	max score	hits	top seq
1	Rattus norvegicus	134.00	1	solute carrier family 17 (sodium-dependent inorga
2	Mus musculus	132.00	2	AF324864_1 vesicular glutamate transporter 2
3	Mus musculus	132.00	1	solute carrier family 17 (sodium-dependent inorga
4	Canis familiaris	131.00	2	PREDICTED: similar to transmembrane protein 16E
5	Bos taurus	126.00	2	solute carrier family 17 (sodium-dependent inorga
6	Homo sapiens	123.00	2	solute carrier family 17 (sodium-dependent inorga
7	Danio rerio	86.30	3	solute carrier family 17 (sodium-dependent inorga
8	N/A	86.30	1	VGL2A_DANRE Vesicular glutamate transporter 2.1 (
9	Tetraodon nigroviridis	83.20	2	unnamed protein product
10	Xenopus laevis	82.00	1	glutamate transporter
11	Rattus norvegicus	38.90	2	vesicular glutamate transporter 3
12	Sus scrofa	32.30	1	spleen tyrosine kinase
13	Nitrosomonas europaea ATCC 19718	31.20	1	hypothetical protein NE0243

Top-ranked sequences

organism	matching
Rattus norvegicus	Query 512 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATSQENGGWPNGWEKKEEFVQESAQD 571 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 512 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATSQENGGWPNGWEKKEEFVQESAQD 571
Mus musculus	Query 512 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATSQENGGWPNGWEKKEEFVQESAQD 571 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| Sbjct 512 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATSQENGGWPNGWEKKEEFVQEGAQD 571
Mus musculus	Query 512 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATSQENGGWPNGWEKKEEFVQESAQD 571 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| Sbjct 512 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATSQENGGWPNGWEKKEEFVQEGAQD 571
Canis familiaris	Query 512 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATSQENGGWPNGWEKKEEFVQESAQD 571 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\| Sbjct 1301 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATTQANGGWPNGWEKKEEFVQEEVQD 1360
Bos taurus	Query 512 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATSQENGGWPNGWEKKEEFVQESAQD 571 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|+ Sbjct 512 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATTQANGGWPNGWEKKEEFVQEEVQN 571
Homo sapiens	Query 512 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATSQENGGWPNGWEKKEEFVQESAQD 571 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|+\| \|\|\|\|\|+\|\|\|\|\|\|\|\|\|\| \|\| Sbjct 512 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATTQANGGWPSGWEKKEEFVQGEVQD 571
Danio rerio	Query 512 SEEKCGFIHEDELDEETGDITQNYINYGT-T#KSYGATSQENGGWPNGWEKKEEFVQESAQ 570 \|+\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|+\| \| #\|\|\|\|\|\|+\| \|\|\|\| \|\|+\|+\|\|+\|\|+ + Sbjct 512 SDEKCGFIDEDELAEETGDITQSYGALGAPA#KSYGATTQLNGGWAEGWDKREEYVQDGVE 571 Query 571 D 571 + Sbjct 572 E 572
N/A	Query 512 SEEKCGFIHEDELDEETGDITQNYINYGT-T#KSYGATSQENGGWPNGWEKKEEFVQESAQ 570 \|+\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|+\| \| #\|\|\|\|\|\|+\| \|\|\|\| \|\|+\|+\|\|+\|\|+ + Sbjct 512 SDEKCGFIDEDELAEETGDITQSYGALGAPA#KSYGATTQLNGGWAEGWDKREEYVQDGVE 571 Query 571 D 571 + Sbjct 572 E 572
Tetraodon nigroviridis	Query 512 SEEKCGFIHEDELDEETGDITQNYINYGT-T#KSYGATSQENGGWPNGWEKKEEFVQESA 569 \|\|\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\| \| #\|\|\|\|\|\|\|\| \|\|\|\| \|+\| \|\|+\|\|\| \| Sbjct 1545 SEEKCGFIDEDELAEETGDITQGYGAMAAPA#KSYGATSQLNGGWVQDWDKTEEYVQEPA 1603
Xenopus laevis	Query 512 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATSQENGGWPNGWEKKEEFVQESAQD 571 \|\|\|\|\|\|\|\|\|\|\|\|\| +\|+ + \|\| + \|\|+ # + \|\|\|+\|\|\|\|\| \|\|\|\|\|\|\|+\|+ +\| Sbjct 504 SEEKCGFIHEDELADESEEQTQAFGGYGSY#GATQTTSQQNGGWPAEWEKKEEFIQDQGKD 563
Rattus norvegicus	Query 512 SEEKCGFIHEDELDEETGDITQNYINYGTT#KSYGATSQ 549 \|\|\|\|\|\| \| +\|\|\| \|\|\| + +++ # \|\|\|\|\|+\| Sbjct 516 SEEKCGIIDQDELAEETELNHEAFVSPRKK#MSYGATTQ 553
Sus scrofa	Query 514 EKCGFIHEDELDEETGDITQNYI---NYGTT#KSYGA-----TSQENGGWPNGWEKKE 562 \|+\| \|+\| \| +\|\|+\| ++\| +#\|+ \| +\| +\| \|\| \| \| Sbjct 479 EECNFVHRDLAARNVLLVTQHYAKISDFGLS#KALRADENYYKAQTHGKWPVKWYAPE 535
Nitrosomonas europaea ATCC 19718	Query 532 TQNYINYGTT#KSYGATSQENGGWPNGWEKKEEFV 565 \|+ \|\| \|\| # \| \| \| \|\| \|\| + \| +\|++ + Sbjct 106 TRRYIKSGTL#HSIGGKSVENVGWTD-WRRKDQIL 138

* References

[PubMed ID: 23543101] Kiss J, Csaba Z, Csaki A, Halasz B, Demonstration of estrogen receptor alpha protein in glutamatergic (vesicular glutamate transporter 2 immunoreactive) neurons of the female rat hypothalamus and amygdala using double-label immunocytochemistry. Exp Brain Res. 2013 May;226(4):595-602. doi: 10.1007/s00221-013-3474-8. Epub 2013

» More (9) « Hide Below

[PubMed ID: 23047588] ... Lei W, Deng Y, Liu B, Mu S, Guley NM, Wong T, Reiner A, Confocal laser scanning microscopy and ultrastructural study of VGLUT2 thalamic input to striatal projection neurons in rats. J Comp Neurol. 2013 Apr 15;521(6):1354-77. doi: 10.1002/cne.23235.

[PubMed ID: 24308494] ... Favier M, Carcenac C, Drui G, Boulet S, El Mestikawy S, Savasta M, High-frequency stimulation of the subthalamic nucleus modifies the expression of vesicular glutamate transporters in basal ganglia in a rat model of Parkinson's disease. BMC Neurosci. 2013 Dec 5;14:152. doi: 10.1186/1471-2202-14-152.

[PubMed ID: 23835161] ... Tukey DS, Lee M, Xu D, Eberle SE, Goffer Y, Manders TR, Ziff EB, Wang J, Differential effects of natural rewards and pain on vesicular glutamate transporter expression in the nucleus accumbens. Mol Brain. 2013 Jul 9;6:32. doi: 10.1186/1756-6606-6-32.

[PubMed ID: 23326507] ... Melo CV, Mele M, Curcio M, Comprido D, Silva CG, Duarte CB, BDNF regulates the expression and distribution of vesicular glutamate transporters in cultured hippocampal neurons. PLoS One. 2013;8(1):e53793. doi: 10.1371/journal.pone.0053793. Epub 2013 Jan 11.

[PubMed ID: 12815758] ... Li JL, Xiong KH, Dong YL, Fujiyama F, Kaneko T, Mizuno N, Vesicular glutamate transporters, VGluT1 and VGluT2, in the trigeminal ganglion neurons of the rat, with special reference to coexpression. J Comp Neurol. 2003 Aug 18;463(2):212-20.

[PubMed ID: 12541308] ... Li JL, Fujiyama F, Kaneko T, Mizuno N, Expression of vesicular glutamate transporters, VGluT1 and VGluT2, in axon terminals of nociceptive primary afferent fibers in the superficial layers of the medullary and spinal dorsal horns of the rat. J Comp Neurol. 2003 Mar 10;457(3):236-49.

[PubMed ID: 12534967] ... Liu YY, Wong-Riley MT, Liu JP, Jia Y, Liu HL, Fujiyama F, Ju G, Relationship between two types of vesicular glutamate transporters and neurokinin-1 receptor-immunoreactive neurons in the pre-Botzinger complex of rats: light and electron microscopic studies. Eur J Neurosci. 2003 Jan;17(1):41-8.

[PubMed ID: 12605886] ... Hioki H, Fujiyama F, Taki K, Tomioka R, Furuta T, Tamamaki N, Kaneko T, Differential distribution of vesicular glutamate transporters in the rat cerebellar cortex. Neuroscience. 2003;117(1):1-6.

[PubMed ID: 11551935] ... Hayashi M, Otsuka M, Morimoto R, Hirota S, Yatsushiro S, Takeda J, Yamamoto A, Moriyama Y, Differentiation-associated Na+-dependent inorganic phosphate cotransporter (DNPI) is a vesicular glutamate transporter in endocrine glutamatergic systems. J Biol Chem. 2001 Nov 16;276(46):43400-6. Epub 2001 Sep 10.