CaMPDB :: Substrate

SB0124 : Actin-binding protein-1, Drebrin-like protein

* Basic Information

Organism	Mus musculus (house mouse)
Protein Names	drebrin-like protein isoform 1 [Mus musculus]; drebrin-like protein isoform 1; drebrin-like protein; actin-binding protein 1; SH3 domain-containing protein 7; dbnl; Drebrin-like protein; Actin-binding protein 1
Gene Names	Dbnl; Abp1 {ECO:0000312\|EMBL:AAM28340.1},Sh3p7 {ECO:0000303\|PubMed:9630982}; Abp1 {ECO:0000312\|EMBL:AAM28340.1}; Sh3p7 {ECO:0000303\|PubMed:9630982}; drebrin-like
Gene Locus	11 3.87 cM; chromosome 11
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_001139780	NM_001146308	13169	Q62418	N/A	N/A	N/A	mmu:13169

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 436 aa

Average Mass: 48.699 kDa

Monoisotopic Mass: 48.670 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
SH3 domain 1.	20	30	24.0	0.0
RNA polymerase I-specific transcription-initiation factor 1.	184	306	745.0	15.5
--- cleavage 283 (inside RNA polymerase I-specific transcription-initiation factor 184..306) ---
--- cleavage 290 (inside RNA polymerase I-specific transcription-initiation factor 184..306) ---
Variant SH3 domain 1.	382	434	2.0	0.0

3. Sequence Information

Fasta Sequence: SB0124.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

2 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 19910490] Cortesio CL, Perrin BJ, Bennin DA, Huttenlocher A, Actin-binding protein-1 interacts with WASp-interacting protein to regulate growth factor-induced dorsal ruffle formation. Mol Biol Cell. 2010 Jan 1;21(1):186-97. doi: 10.1091/mbc.E09-02-0106. Epub 2009

Cleavage sites (±10aa)

[Site 1] RAMSTTSVTS²⁸³-SQPGKLRSPF

Ser²⁸³ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Arg²⁷⁴	Ala²⁷⁵	Met²⁷⁶	Ser²⁷⁷	Thr²⁷⁸	Thr²⁷⁹	Ser²⁸⁰	Val²⁸¹	Thr²⁸²	Ser²⁸³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser²⁸⁴	Gln²⁸⁵	Pro²⁸⁶	Gly²⁸⁷	Lys²⁸⁸	Leu²⁸⁹	Arg²⁹⁰	Ser²⁹¹	Pro²⁹²	Phe²⁹³

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
EWESAGQQAPHPREIFKQKERAMSTTSVTSSQPGKLRSPFLQKQLTQPETSYGREPTAPV

Summary

#	organism	max score	hits	top seq
1	Mus musculus	123.00	7	DBNL_MOUSE Drebrin-like protein (SH3 domain-contai
2	Rattus norvegicus	117.00	4	DBNL_RAT Drebrin-like protein (SH3 domain-containi
3	Homo sapiens	91.70	4	drebrin-like isoform a
4	Homo sapiens	91.70	2	drebrin-like isoform c
5	Pongo pygmaeus	88.20	1	hypothetical protein
6	Canis familiaris	82.80	7	PREDICTED: similar to drebrin-like isoform a isof
7	Xenopus laevis	48.50	1	drebrin-like
8	Tetraodon nigroviridis	37.70	1	unnamed protein product

Top-ranked sequences

organism	matching
Mus musculus	Query 254 EWESAGQQAPHPREIFKQKERAMSTTSVTS#SQPGKLRSPFLQKQLTQPETSYGREPTAPV 313 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 254 EWESAGQQAPHPREIFKQKERAMSTTSVTS#SQPGKLRSPFLQKQLTQPETSYGREPTAPV 313
Rattus norvegicus	Query 254 EWESAGQQAPHPREIFKQKERAMSTTSVTS#SQPGKLRSPFLQKQLTQPETSYGREPTAPV 313 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|+\|\| Sbjct 254 EWESAGQQAPHPREIFKQKERAMSTTSVSS#SQPGKLRSPFLQKQFTQPEASYGREPTSPV 313
Homo sapiens	Query 260 QQAPHPREIFKQKERAMSTTSVTS#SQPGKLRSPFLQKQLTQPETSYGREPTAPV 313 + \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|++\|# \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|\| \| + Sbjct 253 ESAVHPREIFKQKERAMSTTSISS#PQPGKLRSPFLQKQLTQPETHFGREPAAAI 306
Homo sapiens	Query 260 QQAPHPREIFKQKERAMSTTSVTS#SQPGKLRSPFLQKQLTQPETSYGREPTAPV 313 + \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|++\|# \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|\| \| + Sbjct 261 ESAVHPREIFKQKERAMSTTSISS#PQPGKLRSPFLQKQLTQPETHFGREPAAAI 314
Pongo pygmaeus	Query 262 APHPREIFKQKERAMSTTSVTS#SQPGKLRSPFLQKQLTQPETSYGREPTAPV 313 \| +\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|++\|# \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|\| \| \| Sbjct 135 AVYPREIFKQKERAMTTTSISS#PQPGKLRSPFLQKQLTQPETHFGREPAAAV 186
Canis familiaris	Query 254 EWESAGQQAPHPREIFKQKERAMSTTSVTS#SQPGKLRSPFLQKQLTQPETSYGREPT 310 +\| \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|++\|# \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| Sbjct 249 QWREPVSQPAHPREIFKQKERAMSTTSISS#PQPGKLRSPFLQKQLTQPETHRSPEPT 305
Xenopus laevis	Query 258 AGQQAPHPREIFKQKERAMSTTSVTS#SQPGKLRSPFLQKQLTQ 300 \| \|++ +\|\|\| \| \|\|\|\|++ + \|#+\|\|\|+\|\|\|\|\|\|\|\| \| Sbjct 248 ASQRSVNPRETFLQKERSLPESGPVS#AQPGRLRSPFLQKSACQ 290
Tetraodon nigroviridis	Query 260 QQAPHPREIFKQKERAMSTTSVTS#S----QPGKLRSPFL 294 \|+\| +\|\|++\|\|\|+\|\| ++ + +#+ \|\|\|+\|+\|\|\|\| Sbjct 287 QRAVNPRDMFKQRERGVTPSDSDA#APPSPQPGRLQSPFL 325

[Site 2] VTSSQPGKLR²⁹⁰-SPFLQKQLTQ

Arg²⁹⁰ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Val²⁸¹	Thr²⁸²	Ser²⁸³	Ser²⁸⁴	Gln²⁸⁵	Pro²⁸⁶	Gly²⁸⁷	Lys²⁸⁸	Leu²⁸⁹	Arg²⁹⁰

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser²⁹¹	Pro²⁹²	Phe²⁹³	Leu²⁹⁴	Gln²⁹⁵	Lys²⁹⁶	Gln²⁹⁷	Leu²⁹⁸	Thr²⁹⁹	Gln³⁰⁰

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
QAPHPREIFKQKERAMSTTSVTSSQPGKLRSPFLQKQLTQPETSYGREPTAPVSRPAAGV

Summary

#	organism	max score	hits	top seq
1	Mus musculus	123.00	7	unnamed protein product
2	Rattus norvegicus	117.00	4	DBNL_RAT Drebrin-like protein (SH3 domain-containi
3	Homo sapiens	99.00	4	drebrin-like isoform a
4	Homo sapiens	99.00	2	drebrin-like isoform c
5	Pongo pygmaeus	95.10	1	hypothetical protein
6	Canis familiaris	88.20	7	PREDICTED: similar to Drebrin-like protein (SH3 d
7	Xenopus laevis	45.10	1	drebrin-like
8	Tetraodon nigroviridis	36.20	1	unnamed protein product

Top-ranked sequences

organism	matching
Mus musculus	Query 261 QAPHPREIFKQKERAMSTTSVTSSQPGKLR#SPFLQKQLTQPETSYGREPTAPVSRPAAGV 320 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 261 QAPHPREIFKQKERAMSTTSVTSSQPGKLR#SPFLQKQLTQPETSYGREPTAPVSRPAAGV 320
Rattus norvegicus	Query 261 QAPHPREIFKQKERAMSTTSVTSSQPGKLR#SPFLQKQLTQPETSYGREPTAPVSRPAAGV 320 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\| Sbjct 261 QAPHPREIFKQKERAMSTTSVSSSQPGKLR#SPFLQKQFTQPEASYGREPTSPVSRPAAGV 320
Homo sapiens	Query 262 APHPREIFKQKERAMSTTSVTSSQPGKLR#SPFLQKQLTQPETSYGREPTAPVSRPAAGV 320 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|\| \| +\|\|\| \| + Sbjct 255 AVHPREIFKQKERAMSTTSISSPQPGKLR#SPFLQKQLTQPETHFGREPAAAISRPRADL 313
Homo sapiens	Query 262 APHPREIFKQKERAMSTTSVTSSQPGKLR#SPFLQKQLTQPETSYGREPTAPVSRPAAGV 320 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|\| \| +\|\|\| \| + Sbjct 263 AVHPREIFKQKERAMSTTSISSPQPGKLR#SPFLQKQLTQPETHFGREPAAAISRPRADL 321
Pongo pygmaeus	Query 262 APHPREIFKQKERAMSTTSVTSSQPGKLR#SPFLQKQLTQPETSYGREPTAPVSRPAAGV 320 \| +\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|++\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|\| \| \|\|\|\| \| + Sbjct 135 AVYPREIFKQKERAMTTTSISSPQPGKLR#SPFLQKQLTQPETHFGREPAAAVSRPRADL 193
Canis familiaris	Query 262 APHPREIFKQKERAMSTTSVTSSQPGKLR#SPFLQKQLTQPETSYGREPTAPVSRPAAGV 320 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| \|\|\| \| + Sbjct 252 ATHPREIFKQKERAMSTTSISSPQPGKLR#SPFLQKQLTQPETHRSPEPTQASSRPRADI 310
Xenopus laevis	Query 264 HPREIFKQKERAMSTTSVTSSQPGKLR#SPFLQKQLTQ 300 +\|\|\| \| \|\|\|\|++ + \|+\|\|\|+\|\|#\|\|\|\|\|\| \| Sbjct 254 NPRETFLQKERSLPESGPVSAQPGRLR#SPFLQKSACQ 290
Tetraodon nigroviridis	Query 264 HPREIFKQKERAMSTTSV---TSSQPGKLR#SPFLQKQ 297 +\|\|++\|\|\|\|\|+++ + +\|+\|\|\|\|+#\|\|\|\| ++ Sbjct 195 NPRDVFKQKEQSLEAIARPAPAASRPGKLQ#SPFLSQK 231

* References

[PubMed ID: 24958882] Seeley-Fallen MK, Liu LJ, Shapiro MR, Onabajo OO, Palaniyandi S, Zhu X, Tan TH, Upadhyaya A, Song W, Actin-binding protein 1 links B-cell antigen receptors to negative signaling pathways. Proc Natl Acad Sci U S A. 2014 Jul 8;111(27):9881-6. doi:

» More (9) « Hide Below

[PubMed ID: 23223303] ... Haag N, Schwintzer L, Ahuja R, Koch N, Grimm J, Heuer H, Qualmann B, Kessels MM, The actin nucleator Cobl is crucial for Purkinje cell development and works in close conjunction with the F-actin binding protein Abp1. J Neurosci. 2012 Dec 5;32(49):17842-56. doi: 10.1523/JNEUROSCI.0843-12.2012.

[PubMed ID: 22450813] ... Hepper I, Schymeinsky J, Weckbach LT, Jakob SM, Frommhold D, Sixt M, Laschinger M, Sperandio M, Walzog B, The mammalian actin-binding protein 1 is critical for spreading and intraluminal crawling of neutrophils under flow conditions. J Immunol. 2012 May 1;188(9):4590-601. doi: 10.4049/jimmunol.1100878. Epub 2012

[PubMed ID: 22303001] ... Boateng LR, Cortesio CL, Huttenlocher A, Src-mediated phosphorylation of mammalian Abp1 (DBNL) regulates podosome rosette formation in transformed fibroblasts. J Cell Sci. 2012 Mar 1;125(Pt 5):1329-41. doi: 10.1242/jcs.096529. Epub 2012 Feb

[PubMed ID: 21677750] ... Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A, A conditional knockout resource for the genome-wide study of mouse gene function. Nature. 2011 Jun 15;474(7351):337-42. doi: 10.1038/nature10163.

[PubMed ID: 10613908] ... Engqvist-Goldstein AE, Kessels MM, Chopra VS, Hayden MR, Drubin DG, An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles. J Cell Biol. 1999 Dec 27;147(7):1503-18.

[PubMed ID: 10087302] ... Lee CG, Morse HC 3rd, Kay BK, Kozak CA, Genetic mapping of eight SH3 domain genes on seven mouse chromosomes. Mamm Genome. 1999 Apr;10(4):402-4.

[PubMed ID: 9891087] ... Larbolette O, Wollscheid B, Schweikert J, Nielsen PJ, Wienands J, SH3P7 is a cytoskeleton adapter protein and is coupled to signal transduction from lymphocyte antigen receptors. Mol Cell Biol. 1999 Feb;19(2):1539-46.

[PubMed ID: 9126384] ... Yamabhai M, Kay BK, Examining the specificity of Src homology 3 domain--ligand interactions with alkaline phosphatase fusion proteins. Anal Biochem. 1997 Apr 5;247(1):143-51.

[PubMed ID: 9630982] ... Sparks AB, Hoffman NG, McConnell SJ, Fowlkes DM, Kay BK, Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins. Nat Biotechnol. 1996 Jun;14(6):741-4.