logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0127 : Myelin basic protein

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein NamesGolli-MBP isoform 1 [Homo sapiens]; Golli-MBP isoform 1; Golli-MBP; myelin A1 protein; myelin membrane encephalitogenic protein; Myelin basic protein; MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein
Gene NamesMBP; myelin basic protein
Gene Locus18q23; chromosome 18
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_001020272 NM_001025101 4155 P02686 159430 HGNC:6925 01158 hsa:4155

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 304 aa

Average Mass: 33.117 kDa

Monoisotopic Mass: 33.097 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
Myelin basic protein 1. 1537111.00.1
Myelin basic protein 2. 1493041.08.4
--- cleavage 152 (inside Myelin basic protein 149..304) ---
--- cleavage 157 (inside Myelin basic protein 149..304) ---
--- cleavage 161 (inside Myelin basic protein 149..304) ---
--- cleavage 183 (inside Myelin basic protein 149..304) ---
--- cleavage 193 (inside Myelin basic protein 149..304) ---
--- cleavage 204 (inside Myelin basic protein 149..304) ---
--- cleavage 228 (inside Myelin basic protein 149..304) ---
--- cleavage 231 (inside Myelin basic protein 149..304) ---
--- cleavage 244 (inside Myelin basic protein 149..304) ---
--- cleavage 279 (inside Myelin basic protein 149..304) ---
--- cleavage 290 (inside Myelin basic protein 149..304) ---

3. Sequence Information

Fasta Sequence: SB0127.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1BX2 (X-ray; 260 A; C/F=217-231), 1FV1 (X-ray; 190 A; C/F=218-237), 1HQR (X-ray; 320 A; C=221-233), 1K2D (X-ray; 220 A; P=135-144), 1QCL (Model; -; A=135-304), 1YMM (X-ray; 350 A; C=217-240), 1ZGL (X-ray; 280 A; C/F/I/L=221-233)

* Cleavage Information

11 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 7517457] Banik NL, Chou CH, Deibler GE, Krutzch HC, Hogan EL, Peptide bond specificity of calpain: proteolysis of human myelin basic protein. J Neurosci Res. 1994 Mar 1;37(4):489-96.

Cleavage sites (±10aa)

[Site 1] RHGSKYLATA152-STMDHARHGF

Ala152 Ser

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Arg143His144Gly145Ser146Lys147Tyr148Leu149Ala150Thr151Ala152
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ser153Thr154Met155Asp156His157Ala158Arg159His160Gly161Phe162

Sequence conservation (by blast)

[Site 2] YLATASTMDH157-ARHGFLPRHR

His157 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Tyr148Leu149Ala150Thr151Ala152Ser153Thr154Met155Asp156His157
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ala158Arg159His160Gly161Phe162Leu163Pro164Arg165His166Arg167

Sequence conservation (by blast)

[Site 3] ASTMDHARHG161-FLPRHRDTGI

Gly161 Phe

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ala152Ser153Thr154Met155Asp156His157Ala158Arg159His160Gly161
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Phe162Leu163Pro164Arg165His166Arg167Asp168Thr169Gly170Ile171

Sequence conservation (by blast)

[Site 4] SIGRFFGGDR183-GAPKRGSGKD

Arg183 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ser174Ile175Gly176Arg177Phe178Phe179Gly180Gly181Asp182Arg183
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gly184Ala185Pro186Lys187Arg188Gly189Ser190Gly191Lys192Asp193

Sequence conservation (by blast)

[Site 5] GAPKRGSGKD193-SHHPARTAHY

Asp193 Ser

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Gly184Ala185Pro186Lys187Arg188Gly189Ser190Gly191Lys192Asp193
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ser194His195His196Pro197Ala198Arg199Thr200Ala201His202Tyr203

Sequence conservation (by blast)

[Site 6] HHPARTAHYG204-SLPQKSHGRT

Gly204 Ser

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
His195His196Pro197Ala198Arg199Thr200Ala201His202Tyr203Gly204
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ser205Leu206Pro207Gln208Lys209Ser210His211Gly212Arg213Thr214

Sequence conservation (by blast)

[Site 7] PVVHFFKNIV228-TPRTPPPSQG

Val228 Thr

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Pro219Val220Val221His222Phe223Phe224Lys225Asn226Ile227Val228
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Thr229Pro230Arg231Thr232Pro233Pro234Pro235Ser236Gln237Gly238

Sequence conservation (by blast)

[Site 8] HFFKNIVTPR231-TPPPSQGKGR

Arg231 Thr

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
His222Phe223Phe224Lys225Asn226Ile227Val228Thr229Pro230Arg231
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Thr232Pro233Pro234Pro235Ser236Gln237Gly238Lys239Gly240Arg241

Sequence conservation (by blast)

[Site 9] PSQGKGRGLS244-LSRFSWGAEG

Ser244 Leu

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Pro235Ser236Gln237Gly238Lys239Gly240Arg241Gly242Leu243Ser244
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Leu245Ser246Arg247Phe248Ser249Trp250Gly251Ala252Glu253Gly254

Sequence conservation (by blast)

[Site 10] SAHKGFKGVD279-AQGTLSKIFK

Asp279 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ser270Ala271His272Lys273Gly274Phe275Lys276Gly277Val278Asp279
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ala280Gln281Gly282Thr283Leu284Ser285Lys286Ile287Phe288Lys289

Sequence conservation (by blast)

[Site 11] QGTLSKIFKL290-GGRDSRSGSP

Leu290 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Gln281Gly282Thr283Leu284Ser285Lys286Ile287Phe288Lys289Leu290
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gly291Gly292Arg293Asp294Ser295Arg296Ser297Gly298Ser299Pro300

Sequence conservation (by blast)

* References

[PubMed ID: 23726766] Mostafa GA, Al-Ayadhi LY, The possible relationship between allergic manifestations and elevated serum levels of brain specific auto-antibodies in autistic children. J Neuroimmunol. 2013 Aug 15;261(1-2):77-81. doi: 10.1016/j.jneuroim.2013.04.003.

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