CaMPDB :: Substrate

SB0129 : CAPN2, m-calpain catalytic subunit, mCL

* Basic Information

Organism	Rattus norvegicus (Norway rat)
Protein Names	calpain-2 catalytic subunit precursor [Rattus norvegicus]; calpain-2 catalytic subunit precursor; calpain-2 catalytic subunit; M-calpain; calpain M-type; millimolar-calpain; calpain-2 large subunit; calcium-activated neutral proteinase 2; CANP 2; Calpain-2 catalytic subunit; 3.4.22.53; Calcium-activated neutral proteinase 2; Calpain M-type; Calpain-2 large subunit; Millimolar-calpain
Gene Names	Capn2; calpain 2, (m/II) large subunit
Gene Locus	13q26; chromosome 13
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_058812	NM_017116	29154	Q07009	N/A	N/A	N/A	rno:29154

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 700 aa

Average Mass: 79.918 kDa

Monoisotopic Mass: 79.869 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
--- cleavage 1 ---
--- cleavage 2 ---
--- cleavage 3 ---
--- cleavage 7 ---
--- cleavage 9 ---
--- cleavage 10 ---
--- cleavage 11 ---
--- cleavage 12 ---
--- cleavage 13 ---
--- cleavage 14 ---
--- cleavage 23 ---
Calpain family cysteine protease 1.	46	342	1.0	0.0
--- cleavage 60 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 82 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 116 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 201 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 207 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 209 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 247 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 249 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 255 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 267 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 359 ---
--- cleavage 360 ---
--- cleavage 381 ---
--- cleavage 383 ---
--- cleavage 387 ---
--- cleavage 390 ---
--- cleavage 392 ---
--- cleavage 407 ---
--- cleavage 409 ---
--- cleavage 410 ---
--- cleavage 423 ---
CorA-like Mg2+ transporter protein 1.	439	527	67.0	0.1
--- cleavage 449 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 461 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 462 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 468 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 469 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 502 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 509 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 513 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 517 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 524 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 525 (inside CorA-like Mg2+ transporter protein 439..527) ---
EF-hand domain pair 1.	552	598	20.0	0.1
EF-hand domain pair 2.	610	621	31.0	0.0
--- cleavage 611 (inside EF-hand domain pair 610..621) ---
--- cleavage 618 (inside EF-hand domain pair 610..621) ---
EF hand 1.	657	668	17.0	0.0
EF hand 2.	675	680	4.0	0.0

3. Sequence Information

Fasta Sequence: SB0129.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1DF0 (X-ray; 260 A; A=1-700), 1MDW (X-ray; 195 A; A/B=19-346), 1QXP (X-ray; 280 A; A/B=1-700), 1U5I (X-ray; 286 A; A=1-700), 3BOW (X-ray; 240 A; A=1-700), 3DF0 (X-ray; 295 A; A=1-700)

* Cleavage Information

45 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 21549862] Chou JS, Impens F, Gevaert K, Davies PL, m-Calpain activation in vitro does not require autolysis or subunit dissociation. Biochim Biophys Acta. 2011 Jul;1814(7):864-72. doi: 10.1016/j.bbapap.2011.04.007.

Cleavage sites (±10aa)

[Site 1] M¹-AGIAMKLAKD

Met¹ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

-	-	-	-	-	-	-	-	-	Met¹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala²	Gly³	Ile⁴	Ala⁵	Met⁶	Lys⁷	Leu⁸	Ala⁹	Lys¹⁰	Asp¹¹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAMKLAKDREAAEGLGSHERAIKYLNQD

#	organism	max score	hits	top seq
1	N/A	65.90	9	A Chain A, Crystal Structure Of M-Calpain
2	Rattus norvegicus	65.90	4	calpain 2
3	Mus musculus	64.30	14	m-calpain large subunit
4	Homo sapiens	63.50	4	AF261089_1 calpain large polypeptide L2
5	Homo sapiens	63.50	2	calpain 2, large subunit
6	Macaca mulatta	62.40	2	PREDICTED: calpain 2, large subunit
7	Xenopus laevis	45.80	3	calpain 8
8	Coturnix coturnix	42.40	1	quail calpain
9	Gallus gallus	42.40	1	calpain 1, (mu/I) large subunit
10	Canis familiaris	42.00	3	PREDICTED: similar to calpain 8
11	Xenopus tropicalis	41.20	4	calpain 2, (m/II) large subunit
12	Stizostedion vitreum vitreum	39.30	1	putative calpain-like protein
13	Oncorhynchus mykiss	39.30	1	m-calpain
14	Xenopus laevis	38.50	1	mu/m-calpain large subunit
15	Tetraodon nigroviridis	38.10	4	unnamed protein product
16	Danio rerio	38.10	3	calpain 2, (m/II) large subunit a
17	Rattus norvegicus	36.60	1	calpain 1, large subunit
18	Bos taurus	35.40	2	micromolar calcium activated neutral protease 1
19	synthetic construct	35.40	1	Homo sapiens calpain 1, (mu/I) large subunit
20	Pongo pygmaeus	35.00	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
21	Sus scrofa	33.50	1	calpain 1, large subunit

organism	matching
N/A	Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31
Rattus norvegicus	Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31
Mus musculus	Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|#\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 M#AGIAIKLAKDREAAEGLGSHERAIKYLNQD 31
Homo sapiens	Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|#\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 M#AGIAAKLAKDREAAEGLGSHERAIKYLNQD 31
Homo sapiens	Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|#\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 M#AGIAAKLAKDREAAEGLGSHERAIKYLNQD 31
Macaca mulatta	Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|#\|\|\|\| \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 M#AGIAAKLVKDREAAEGLGSHERAIKYLNQD 31
Xenopus laevis	Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|#+\|\|\| \|+\|\|\|\| \|+\|\|\|++++ +\|\|\|\|\|\| Sbjct 1 M#SGIASKVAKDRVLAQGLGTNDKPLKYLNQD 31
Coturnix coturnix	Query 2 AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|\|\| +\| +\|\| \|\|\|+\| \| \|+\|\|\|\|\|\| Sbjct 5 GGIAARLQRDRLRAEGVGEHNNAVKYLNQD 34
Gallus gallus	Query 2 AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|\|\| +\| +\|\| \|\|\|+\| \| \|+\|\|\|\|\|\| Sbjct 5 GGIAARLQRDRLRAEGVGEHNNAVKYLNQD 34
Canis familiaris	Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|#+ +\| ++\|\| + \|\| \|\|\|\|\|++\|+\|\|\| \|\| Sbjct 94 M#SALAARIAKQQAAAGGLGSHQKAVKYLGQD 124
Xenopus tropicalis	Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|#+\|+\| +\|\|\|+\| \| \|\|\|++\|+\|+ \|\|+\|\| Sbjct 1 M#SGVADRLAKERALASGLGTNEKALPYLSQD 31
Stizostedion vitreum vitreum	Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|#+\|\|\| \| + \|\| +\|+\|\| +\|+\|\|\|\|\|\| Sbjct 1 M#SGIAATLQRRREKEQGIGSCSQAVKYLNQD 31
Oncorhynchus mykiss	Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|#+\|+\| \|\|\| \| \| \| \|++ \|+\|\|\|\|\|\| Sbjct 1 M#SGMASNLAKKRALAAGFGTNANAVKYLNQD 31
Xenopus laevis	Query 2 AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|\|\| \|\| \|\| \| \|+\|\|\|+ \|+\|\| \|\|\| Sbjct 5 GGIASKLKIDRLKASGVGSHDCAVKYQNQD 34
Tetraodon nigroviridis	Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|#+\|\|\| \|\| \|+ \|+\| \|+ +\| \|\|\|\|\|\| Sbjct 1 M#SGIAAKLQHHRDRAQGFGTASQAQKYLNQD 31
Danio rerio	Query 1 M#AGIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|#+\|+\| \|\|\| \| \| \| \|++ \|+\|\|\|\|\|+ Sbjct 1 M#SGVASTLAKKRALAAGFGTNSNAVKYLNQN 31
Rattus norvegicus	Query 3 GIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|++ ++ \| \|+ \|\|\| \|\| \|\|\|\|\| \|\| Sbjct 13 GVSAQVQKQRDKELGLGRHENAIKYLGQD 41
Bos taurus	Query 3 GIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|++ ++ \| \| \|\|\| \|\| \|\|\|\|\| \|\| Sbjct 13 GVSAQVQKQRAKELGLGRHENAIKYLGQD 41
synthetic construct	Query 3 GIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|++ ++ \| \| \|\|\| \|\| \|\|\|\|\| \|\| Sbjct 13 GVSAQVQKQRARELGLGRHENAIKYLGQD 41
Pongo pygmaeus	Query 3 GIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|++ ++ \| \| \|\|\| \|\| \|\|\|\|\| \|\| Sbjct 13 GVSAQVQKQRARELGLGRHENAIKYLGQD 41
Sus scrofa	Query 3 GIAMKLAKDREAAEGLGSHERAIKYLNQD 31 \|++ ++ \| \| \|\|\| \|\| \|\|\|\|\| \|\| Sbjct 13 GVSAQVQKLRAKELGLGRHENAIKYLGQD 41

[Site 2] MA²-GIAMKLAKDR

Ala² Gly

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

-	-	-	-	-	-	-	-	Met¹	Ala²

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gly³	Ile⁴	Ala⁵	Met⁶	Lys⁷	Leu⁸	Ala⁹	Lys¹⁰	Asp¹¹	Arg¹²

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAMKLAKDREAAEGLGSHERAIKYLNQDY

#	organism	max score	hits	top seq
1	N/A	68.90	10	A Chain A, Crystal Structure Of M-Calpain
2	Rattus norvegicus	68.90	4	calpain 2
3	Mus musculus	67.40	14	m-calpain
4	Homo sapiens	66.20	4	AF261089_1 calpain large polypeptide L2
5	Homo sapiens	66.20	2	calpain 2, large subunit
6	Macaca mulatta	65.10	2	PREDICTED: calpain 2, large subunit
7	Xenopus laevis	48.50	3	calpain 8
8	Coturnix coturnix	45.40	1	quail calpain
9	Gallus gallus	45.40	1	calpain 1, (mu/I) large subunit
10	Xenopus tropicalis	43.90	4	calpain 2, (m/II) large subunit
11	Canis familiaris	43.50	3	PREDICTED: similar to calpain 8
12	Stizostedion vitreum vitreum	42.40	1	putative calpain-like protein
13	Xenopus laevis	41.20	1	mu/m-calpain large subunit
14	Oncorhynchus mykiss	40.40	1	m-calpain
15	Danio rerio	39.70	5	PREDICTED: similar to Calpain 1, (mu/I) large sub
16	Tetraodon nigroviridis	39.30	5	unnamed protein product
17	Rattus norvegicus	39.30	1	calpain 1, large subunit
18	Bos taurus	38.10	2	micromolar calcium activated neutral protease 1
19	synthetic construct	38.10	1	Homo sapiens calpain 1, (mu/I) large subunit
20	Pongo pygmaeus	37.70	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
21	Sus scrofa	36.20	1	calpain 1, large subunit
22	Gallus gallus	32.30	1	mu-calpain large subunit

organism	matching
N/A	Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32
Rattus norvegicus	Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32
Mus musculus	Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|\|#\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MA#GIAIKLAKDREAAEGLGSHERAIKYLNQDY 32
Homo sapiens	Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|\|#\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MA#GIAAKLAKDREAAEGLGSHERAIKYLNQDY 32
Homo sapiens	Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|\|#\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MA#GIAAKLAKDREAAEGLGSHERAIKYLNQDY 32
Macaca mulatta	Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|\|#\|\|\| \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MA#GIAAKLVKDREAAEGLGSHERAIKYLNQDY 32
Xenopus laevis	Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|+#\|\|\| \|+\|\|\|\| \|+\|\|\|++++ +\|\|\|\|\|\|\| Sbjct 1 MS#GIASKVAKDRVLAQGLGTNDKPLKYLNQDY 32
Coturnix coturnix	Query 2 A#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 #\|\|\| +\| +\|\| \|\|\|+\| \| \|+\|\|\|\|\|\|\| Sbjct 5 G#GIAARLQRDRLRAEGVGEHNNAVKYLNQDY 35
Gallus gallus	Query 2 A#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 #\|\|\| +\| +\|\| \|\|\|+\| \| \|+\|\|\|\|\|\|\| Sbjct 5 G#GIAARLQRDRLRAEGVGEHNNAVKYLNQDY 35
Xenopus tropicalis	Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|+#\|+\| +\|\|\|+\| \| \|\|\|++\|+\|+ \|\|+\|\|\| Sbjct 1 MS#GVADRLAKERALASGLGTNEKALPYLSQDY 32
Canis familiaris	Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|+# +\| ++\|\| + \|\| \|\|\|\|\|++\|+\|\|\| \|\|+ Sbjct 94 MS#ALAARIAKQQAAAGGLGSHQKAVKYLGQDF 125
Stizostedion vitreum vitreum	Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|+#\|\|\| \| + \|\| +\|+\|\| +\|+\|\|\|\|\|\|\| Sbjct 1 MS#GIAATLQRRREKEQGIGSCSQAVKYLNQDY 32
Xenopus laevis	Query 2 A#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 #\|\|\| \|\| \|\| \| \|+\|\|\|+ \|+\|\| \|\|\|\| Sbjct 5 G#GIASKLKIDRLKASGVGSHDCAVKYQNQDY 35
Oncorhynchus mykiss	Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|+#\|+\| \|\|\| \| \| \| \|++ \|+\|\|\|\|\|\|+ Sbjct 1 MS#GMASNLAKKRALAAGFGTNANAVKYLNQDF 32
Danio rerio	Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 + #\|++ ++ \|+\| \|\|\|+\|+++ \|\|\|+\|\|\|\|\| Sbjct 4 IG#GVSTRIYKERLQAEGMGANDLAIKFLNQDY 35
Tetraodon nigroviridis	Query 1 MA#GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|+#\|\|\| \|\| \|+ \|+\| \|+ +\| \|\|\|\|\|\|+ Sbjct 1 MS#GIAAKLQHHRDRAQGFGTASQAQKYLNQDF 32
Rattus norvegicus	Query 3 GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|++ ++ \| \|+ \|\|\| \|\| \|\|\|\|\| \|\|\| Sbjct 13 GVSAQVQKQRDKELGLGRHENAIKYLGQDY 42
Bos taurus	Query 3 GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|++ ++ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\| Sbjct 13 GVSAQVQKQRAKELGLGRHENAIKYLGQDY 42
synthetic construct	Query 3 GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|++ ++ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\| Sbjct 13 GVSAQVQKQRARELGLGRHENAIKYLGQDY 42
Pongo pygmaeus	Query 3 GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|++ ++ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\| Sbjct 13 GVSAQVQKQRARELGLGRHENAIKYLGQDY 42
Sus scrofa	Query 3 GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|++ ++ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\| Sbjct 13 GVSAQVQKLRAKELGLGRHENAIKYLGQDY 42
Gallus gallus	Query 3 GIAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|++ ++ + \| \| \|\|\| \|+ \|+++ \|\|\| Sbjct 13 GVSAQVQRQRAKALGLGQHQNAVRFRGQDY 42

[Site 3] MAG³-IAMKLAKDRE

Gly³ Ile

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

-	-	-	-	-	-	-	Met¹	Ala²	Gly³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ile⁴	Ala⁵	Met⁶	Lys⁷	Leu⁸	Ala⁹	Lys¹⁰	Asp¹¹	Arg¹²	Glu¹³

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAMKLAKDREAAEGLGSHERAIKYLNQDYE

#	organism	max score	hits	top seq
1	N/A	70.10	11	A Chain A, Crystal Structure Of A Mu-Like Calpain
2	Rattus norvegicus	69.70	4	calpain 2
3	Mus musculus	68.20	14	m-calpain
4	Homo sapiens	67.40	4	AF261089_1 calpain large polypeptide L2
5	Homo sapiens	67.40	2	calpain 2, large subunit
6	Macaca mulatta	66.20	2	PREDICTED: calpain 2, large subunit
7	Xenopus laevis	50.40	3	calpain 8
8	Coturnix coturnix	47.00	1	quail calpain
9	Gallus gallus	47.00	1	calpain 1, (mu/I) large subunit
10	Xenopus tropicalis	45.80	4	calpain 2, (m/II) large subunit
11	Canis familiaris	45.40	3	PREDICTED: similar to calpain 8
12	Stizostedion vitreum vitreum	43.90	1	putative calpain-like protein
13	Xenopus laevis	42.70	1	mu/m-calpain large subunit
14	Oncorhynchus mykiss	42.40	1	m-calpain
15	Danio rerio	41.20	5	calpain 2, (m/II) large subunit a
16	Tetraodon nigroviridis	40.80	5	unnamed protein product
17	Rattus norvegicus	40.80	1	calpain 1, large subunit
18	Bos taurus	40.00	2	calpain 1, (mu/I) large subunit
19	synthetic construct	39.70	1	Homo sapiens calpain 1, (mu/I) large subunit
20	Pongo pygmaeus	39.70	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
21	Sus scrofa	38.10	1	calpain 1, large subunit
22	Gallus gallus	32.00	1	mu-calpain large subunit
23	Canis familiaris	31.20	1	PREDICTED: similar to Calpain-11 (Calcium-activat
24	Danio rerio	30.40	1	calpain 1, large subunit

organism	matching
N/A	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33
Rattus norvegicus	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33
Mus musculus	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|\|\|#\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAG#IAIKLAKDREAAEGLGSHERAIKYLNQDYE 33
Homo sapiens	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|\|\|#\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAG#IAAKLAKDREAAEGLGSHERAIKYLNQDYE 33
Homo sapiens	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|\|\|#\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAG#IAAKLAKDREAAEGLGSHERAIKYLNQDYE 33
Macaca mulatta	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|\|\|#\|\| \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAG#IAAKLVKDREAAEGLGSHERAIKYLNQDYE 33
Xenopus laevis	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|+\|#\|\| \|+\|\|\|\| \|+\|\|\|++++ +\|\|\|\|\|\|\|\| Sbjct 1 MSG#IASKVAKDRVLAQGLGTNDKPLKYLNQDYE 33
Coturnix coturnix	Query 2 AG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|#\|\| +\| +\|\| \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| Sbjct 5 GG#IAARLQRDRLRAEGVGEHNNAVKYLNQDYE 36
Gallus gallus	Query 2 AG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|#\|\| +\| +\|\| \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| Sbjct 5 GG#IAARLQRDRLRAEGVGEHNNAVKYLNQDYE 36
Xenopus tropicalis	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|+\|#+\| +\|\|\|+\| \| \|\|\|++\|+\|+ \|\|+\|\|\|\| Sbjct 1 MSG#VADRLAKERALASGLGTNEKALPYLSQDYE 33
Canis familiaris	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|+ #+\| ++\|\| + \|\| \|\|\|\|\|++\|+\|\|\| \|\|+\| Sbjct 94 MSA#LAARIAKQQAAAGGLGSHQKAVKYLGQDFE 126
Stizostedion vitreum vitreum	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|+\|#\|\| \| + \|\| +\|+\|\| +\|+\|\|\|\|\|\|\|\| Sbjct 1 MSG#IAATLQRRREKEQGIGSCSQAVKYLNQDYE 33
Xenopus laevis	Query 2 AG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|#\|\| \|\| \|\| \| \|+\|\|\|+ \|+\|\| \|\|\|\|\| Sbjct 5 GG#IASKLKIDRLKASGVGSHDCAVKYQNQDYE 36
Oncorhynchus mykiss	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|+\|#+\| \|\|\| \| \| \| \|++ \|+\|\|\|\|\|\|+\| Sbjct 1 MSG#MASNLAKKRALAAGFGTNANAVKYLNQDFE 33
Danio rerio	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|+\|#+\| \|\|\| \| \| \| \|++ \|+\|\|\|\|\|++\| Sbjct 1 MSG#VASTLAKKRALAAGFGTNSNAVKYLNQNFE 33
Tetraodon nigroviridis	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|+\|#\|\| \|\| \|+ \|+\| \|+ +\| \|\|\|\|\|\|+\| Sbjct 1 MSG#IAAKLQHHRDRAQGFGTASQAQKYLNQDFE 33
Rattus norvegicus	Query 3 G#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|#++ ++ \| \|+ \|\|\| \|\| \|\|\|\|\| \|\|\|\| Sbjct 13 G#VSAQVQKQRDKELGLGRHENAIKYLGQDYE 43
Bos taurus	Query 3 G#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|#++ ++ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| Sbjct 13 G#VSAQVQKQRAKELGLGRHENAIKYLGQDYE 43
synthetic construct	Query 3 G#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|#++ ++ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| Sbjct 13 G#VSAQVQKQRARELGLGRHENAIKYLGQDYE 43
Pongo pygmaeus	Query 3 G#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|#++ ++ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| Sbjct 13 G#VSAQVQKQRARELGLGRHENAIKYLGQDYE 43
Sus scrofa	Query 3 G#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|#++ ++ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| Sbjct 13 G#VSAQVQKLRAKELGLGRHENAIKYLGQDYE 43
Gallus gallus	Query 3 G#IAMKLAKDREAAEGLGSHERAIKYLNQDY 32 \|#++ ++ + \| \| \|\|\| \|+ \|+++ \|\|\| Sbjct 13 G#VSAQVQRQRAKALGLGQHQNAVRFRGQDY 42
Canis familiaris	Query 1 MAG#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 + \|#+\| ++ \| \|+\|\|\| \| \| \| \|\|++\| Sbjct 83 LEG#MAAHISHSRLKAKGLGQHHNAQNYNNQNFE 115
Danio rerio	Query 3 G#IAMKLAKDREAAEGLGSHERAIKYLNQDYE 33 \|#+\| +\| + \|\|\| + \|+\|+\| \|\|\|\| Sbjct 8 G#MAARLRSQWDRDAGLGQNHNAVKFLGQDYE 38

[Site 4] MAGIAMK⁷-LAKDREAAEG

Lys⁷ Leu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

-	-	-	Met¹	Ala²	Gly³	Ile⁴	Ala⁵	Met⁶	Lys⁷

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Leu⁸	Ala⁹	Lys¹⁰	Asp¹¹	Arg¹²	Glu¹³	Ala¹⁴	Ala¹⁵	Glu¹⁶	Gly¹⁷

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAMKLAKDREAAEGLGSHERAIKYLNQDYETLRN

#	organism	max score	hits	top seq
1	N/A	78.20	11	A Chain A, Crystal Structure Of M-Calpain
2	Rattus norvegicus	78.20	4	calpain 2
3	Mus musculus	76.60	14	m-calpain
4	Homo sapiens	74.30	4	AF261089_1 calpain large polypeptide L2
5	Homo sapiens	74.30	2	calpain 2, large subunit
6	Macaca mulatta	72.80	2	PREDICTED: calpain 2, large subunit
7	Xenopus laevis	52.80	4	calpain 8
8	Xenopus tropicalis	52.00	4	calpain 2, (m/II) large subunit
9	Canis familiaris	49.30	3	PREDICTED: similar to calpain 8
10	Coturnix coturnix	49.30	1	quail calpain
11	Gallus gallus	49.30	1	calpain 1, (mu/I) large subunit
12	Stizostedion vitreum vitreum	47.80	1	putative calpain-like protein
13	Danio rerio	47.40	5	calpain 2, (m/II) large subunit a
14	Oncorhynchus mykiss	46.20	1	m-calpain
15	Xenopus laevis	46.20	1	mu/m-calpain large subunit
16	Tetraodon nigroviridis	45.40	5	unnamed protein product
17	Rattus norvegicus	44.70	1	calpain 1, large subunit
18	Bos taurus	43.50	2	micromolar calcium activated neutral protease 1
19	synthetic construct	43.10	1	Homo sapiens calpain 1, (mu/I) large subunit
20	Pongo pygmaeus	43.10	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
21	Sus scrofa	41.60	1	calpain 1, large subunit
22	Gallus gallus	36.20	1	mu-calpain large subunit
23	Danio rerio	35.80	1	calpain 1, large subunit
24	Canis familiaris	33.10	1	PREDICTED: similar to Calpain-11 (Calcium-activat

organism	matching
N/A	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLRN 37 \|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLRN 37
Rattus norvegicus	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLRN 37 \|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLRN 37
Mus musculus	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLRN 37 \|\|\|\|\|+\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAIK#LAKDREAAEGLGSHERAIKYLNQDYETLRN 37
Homo sapiens	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLRN 37 \|\|\|\|\| \|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| Sbjct 1 MAGIAAK#LAKDREAAEGLGSHERAIKYLNQDYEALRN 37
Homo sapiens	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLRN 37 \|\|\|\|\| \|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| Sbjct 1 MAGIAAK#LAKDREAAEGLGSHERAIKYLNQDYEALRN 37
Macaca mulatta	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLRN 37 \|\|\|\|\| \|#\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| Sbjct 1 MAGIAAK#LVKDREAAEGLGSHERAIKYLNQDYEALRN 37
Xenopus laevis	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|+\|\|\| \|#+\|\|\|\| \|+\|\|\|++++ +\|\|\|\|\|\|\|\| \|+ Sbjct 1 MSGIASK#VAKDRVLAQGLGTNDKPLKYLNQDYEQLK 36
Xenopus tropicalis	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLRN 37 \|+\|+\| +#\|\|\|+\| \| \|\|\|++\|+\|+ \|\|+\|\|\|\| \|\|\| Sbjct 1 MSGVADR#LAKERALASGLGTNEKALPYLSQDYEALRN 37
Canis familiaris	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|+ +\| +#+\|\| + \|\| \|\|\|\|\|++\|+\|\|\| \|\|+\| \|\| Sbjct 94 MSALAAR#IAKQQAAAGGLGSHQKAVKYLGQDFEKLR 129
Coturnix coturnix	Query 2 AGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|\|\| +#\| +\|\| \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ Sbjct 5 GGIAAR#LQRDRLRAEGVGEHNNAVKYLNQDYEALK 39
Gallus gallus	Query 2 AGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|\|\| +#\| +\|\| \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ Sbjct 5 GGIAAR#LQRDRLRAEGVGEHNNAVKYLNQDYEALK 39
Stizostedion vitreum vitreum	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|+\|\|\| #\| + \|\| +\|+\|\| +\|+\|\|\|\|\|\|\|\| \|\| Sbjct 1 MSGIAAT#LQRRREKEQGIGSCSQAVKYLNQDYEALR 36
Danio rerio	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLRN 37 \|+\|+\| #\|\|\| \| \| \| \|++ \|+\|\|\|\|\|++\|\|\|\|+ Sbjct 1 MSGVAST#LAKKRALAAGFGTNSNAVKYLNQNFETLRS 37
Oncorhynchus mykiss	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|+\|+\| #\|\|\| \| \| \| \|++ \|+\|\|\|\|\|\|+\| \|\| Sbjct 1 MSGMASN#LAKKRALAAGFGTNANAVKYLNQDFEALR 36
Xenopus laevis	Query 2 AGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|\|\| \|#\| \|\| \| \|+\|\|\|+ \|+\|\| \|\|\|\|\|+\|+ Sbjct 5 GGIASK#LKIDRLKASGVGSHDCAVKYQNQDYESLK 39
Tetraodon nigroviridis	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|+\|\|\| \|#\| \|+ \|+\| \|+ +\| \|\|\|\|\|\|+\|+\|\| Sbjct 1 MSGIAAK#LQHHRDRAQGFGTASQAQKYLNQDFESLR 36
Rattus norvegicus	Query 3 GIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|++ +#+ \| \|+ \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| Sbjct 13 GVSAQ#VQKQRDKELGLGRHENAIKYLGQDYENLR 46
Bos taurus	Query 3 GIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|++ +#+ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| Sbjct 13 GVSAQ#VQKQRAKELGLGRHENAIKYLGQDYEQLR 46
synthetic construct	Query 3 GIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|++ +#+ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| Sbjct 13 GVSAQ#VQKQRARELGLGRHENAIKYLGQDYEQLR 46
Pongo pygmaeus	Query 3 GIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|++ +#+ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| Sbjct 13 GVSAQ#VQKQRARELGLGRHENAIKYLGQDYEQLR 46
Sus scrofa	Query 3 GIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|++ +#+ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| Sbjct 13 GVSAQ#VQKLRAKELGLGRHENAIKYLGQDYEQLR 46
Gallus gallus	Query 3 GIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLRN 37 \|++ +#+ + \| \| \|\|\| \|+ \|+++ \|\|\| \|\|+ Sbjct 13 GVSAQ#VQRQRAKALGLGQHQNAVRFRGQDYAALRD 47
Danio rerio	Query 3 GIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 \|+\| +#\| + \|\|\| + \|+\|+\| \|\|\|\|\|\|\| Sbjct 8 GMAAR#LRSQWDRDAGLGQNHNAVKFLGQDYETLR 41
Canis familiaris	Query 1 MAGIAMK#LAKDREAAEGLGSHERAIKYLNQDYETLR 36 + \|+\| #++ \| \|+\|\|\| \| \| \| \|\|++\| \|+ Sbjct 83 LEGMAAH#ISHSRLKAKGLGQHHNAQNYNNQNFEDLQ 118

[Site 5] MAGIAMKLA⁹-KDREAAEGLG

Ala⁹ Lys

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

-	Met¹	Ala²	Gly³	Ile⁴	Ala⁵	Met⁶	Lys⁷	Leu⁸	Ala⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Lys¹⁰	Asp¹¹	Arg¹²	Glu¹³	Ala¹⁴	Ala¹⁵	Glu¹⁶	Gly¹⁷	Leu¹⁸	Gly¹⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAMKLAKDREAAEGLGSHERAIKYLNQDYETLRNEC

#	organism	max score	hits	top seq
1	N/A	84.00	11	A Chain A, Crystal Structure Of A Mu-Like Calpain
2	Rattus norvegicus	83.20	4	calpain 2
3	Mus musculus	81.60	14	unnamed protein product
4	Homo sapiens	79.30	4	AF261089_1 calpain large polypeptide L2
5	Homo sapiens	79.30	2	calpain 2, large subunit
6	Macaca mulatta	77.80	2	PREDICTED: calpain 2, large subunit
7	Xenopus tropicalis	57.40	4	calpain 2, (m/II) large subunit
8	Gallus gallus	55.10	1	calpain 1, (mu/I) large subunit
9	Xenopus laevis	54.70	4	calpain 8
10	Canis familiaris	53.90	3	PREDICTED: similar to calpain 8
11	Danio rerio	52.80	6	calpain 2, (m/II) large subunit a
12	Coturnix coturnix	52.80	1	quail calpain
13	Stizostedion vitreum vitreum	50.80	1	putative calpain-like protein
14	Xenopus laevis	50.40	2	mu/m-calpain large subunit
15	Oncorhynchus mykiss	50.10	1	m-calpain
16	Tetraodon nigroviridis	48.10	6	unnamed protein product
17	Rattus norvegicus	47.80	1	calpain 1, large subunit
18	Bos taurus	46.20	2	micromolar calcium activated neutral protease 1
19	synthetic construct	45.80	1	Homo sapiens calpain 1, (mu/I) large subunit
20	Pongo pygmaeus	45.40	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
21	Sus scrofa	44.70	1	calpain 1, large subunit
22	Gallus gallus	40.40	1	mu-calpain large subunit
23	Danio rerio	36.20	1	calpain 1, large subunit
24	Canis familiaris	35.80	1	PREDICTED: similar to Calpain-11 (Calcium-activat

organism	matching
N/A	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39
Rattus norvegicus	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39
Mus musculus	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|\|\|+\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39
Homo sapiens	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|\|\| \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39
Homo sapiens	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|\|\| \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39
Macaca mulatta	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|\|\| \|\| #\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 1 MAGIAAKLV#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39
Xenopus tropicalis	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+\|+\| +\|\|#\|+\| \| \|\|\|++\|+\|+ \|\|+\|\|\|\| \|\|\|\|\| Sbjct 1 MSGVADRLA#KERALASGLGTNEKALPYLSQDYEALRNEC 39
Gallus gallus	Query 2 AGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\| +\| #+\|\| \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ \|\| Sbjct 5 GGIAARLQ#RDRLRAEGVGEHNNAVKYLNQDYEALKQEC 42
Xenopus laevis	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+\|\|\| \|+\|#\|\|\| \|+\|\|\|++++ +\|\|\|\|\|\|\|\| \|+ \| Sbjct 1 MSGIASKVA#KDRVLAQGLGTNDKPLKYLNQDYEQLKARC 39
Canis familiaris	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+ +\| ++\|#\| + \|\| \|\|\|\|\|++\|+\|\|\| \|\|+\| \|\| +\| Sbjct 94 MSALAARIA#KQQAAAGGLGSHQKAVKYLGQDFEKLRQQC 132
Danio rerio	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+\|+\| \|\|#\| \| \| \| \|++ \|+\|\|\|\|\|++\|\|\|\|+\|\| Sbjct 1 MSGVASTLA#KKRALAAGFGTNSNAVKYLNQNFETLRSEC 39
Coturnix coturnix	Query 2 AGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\| +\| #+\|\| \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ \| Sbjct 5 GGIAARLQ#RDRLRAEGVGEHNNAVKYLNQDYEALKQAC 42
Stizostedion vitreum vitreum	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+\|\|\| \| #+ \|\| +\|+\|\| +\|+\|\|\|\|\|\|\|\| \|\| \| Sbjct 1 MSGIAATLQ#RRREKEQGIGSCSQAVKYLNQDYEALRRRC 39
Xenopus laevis	Query 2 AGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\| \|\| # \|\| \| \|+\|\|\|+ \|+\|\| \|\|\|\|\|+\|+ +\| Sbjct 5 GGIASKLK#IDRLKASGVGSHDCAVKYQNQDYESLKQQC 42
Oncorhynchus mykiss	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+\|+\| \|\|#\| \| \| \| \|++ \|+\|\|\|\|\|\|+\| \|\| +\| Sbjct 1 MSGMASNLA#KKRALAAGFGTNANAVKYLNQDFEALRAQC 39
Tetraodon nigroviridis	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+\|\|\| \|\| # \|+ \|+\| \|+ +\| \|\|\|\|\|\|+\|+\|\| \| Sbjct 1 MSGIAAKLQ#HHRDRAQGFGTASQAQKYLNQDFESLRESC 39
Rattus norvegicus	Query 3 GIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|++ ++ #\| \|+ \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \| Sbjct 13 GVSAQVQ#KQRDKELGLGRHENAIKYLGQDYENLRARC 49
Bos taurus	Query 3 GIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|++ ++ #\| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \| Sbjct 13 GVSAQVQ#KQRAKELGLGRHENAIKYLGQDYEQLRVHC 49
synthetic construct	Query 3 GIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|++ ++ #\| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \| Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49
Pongo pygmaeus	Query 3 GIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|++ ++ #\| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \| Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49
Sus scrofa	Query 3 GIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|++ ++ #\| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \| Sbjct 13 GVSAQVQ#KLRAKELGLGRHENAIKYLGQDYEQLRAHC 49
Gallus gallus	Query 3 GIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|++ ++ #+ \| \| \|\|\| \|+ \|+++ \|\|\| \|\|++\| Sbjct 13 GVSAQVQ#RQRAKALGLGQHQNAVRFRGQDYAALRDDC 49
Danio rerio	Query 3 GIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNE 38 \|+\| +\| # + \|\|\| + \|+\|+\| \|\|\|\|\|\|\| + Sbjct 8 GMAARLR#SQWDRDAGLGQNHNAVKFLGQDYETLRAQ 43
Canis familiaris	Query 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 + \|+\| ++# \| \|+\|\|\| \| \| \| \|\|++\| \|+ \| Sbjct 83 LEGMAAHIS#HSRLKAKGLGQHHNAQNYNNQNFEDLQAAC 121

[Site 6] MAGIAMKLAK¹⁰-DREAAEGLGS

Lys¹⁰ Asp

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Met¹	Ala²	Gly³	Ile⁴	Ala⁵	Met⁶	Lys⁷	Leu⁸	Ala⁹	Lys¹⁰

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Asp¹¹	Arg¹²	Glu¹³	Ala¹⁴	Ala¹⁵	Glu¹⁶	Gly¹⁷	Leu¹⁸	Gly¹⁹	Ser²⁰

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAMKLAKDREAAEGLGSHERAIKYLNQDYETLRNECL

#	organism	max score	hits	top seq
1	N/A	85.90	12	A Chain A, Crystal Structure Of A Mu-Like Calpain
2	Rattus norvegicus	85.10	5	calpain 2
3	Mus musculus	83.60	14	m-calpain
4	Homo sapiens	81.30	6	AF261089_1 calpain large polypeptide L2
5	Homo sapiens	81.30	2	calpain 2, large subunit
6	Macaca mulatta	79.70	2	PREDICTED: calpain 2, large subunit
7	Xenopus tropicalis	59.30	4	calpain 2, (m/II) large subunit
8	Xenopus laevis	56.60	4	calpain 8
9	Gallus gallus	55.80	1	calpain 1, (mu/I) large subunit
10	Canis familiaris	55.50	3	PREDICTED: similar to calpain 8
11	Danio rerio	54.70	6	calpain 2, (m/II) large subunit a
12	Coturnix coturnix	53.50	1	quail calpain
13	Stizostedion vitreum vitreum	52.80	1	putative calpain-like protein
14	Oncorhynchus mykiss	52.00	1	m-calpain
15	Xenopus laevis	50.80	2	mu/m-calpain large subunit
16	Tetraodon nigroviridis	50.10	7	unnamed protein product
17	Rattus norvegicus	49.30	1	calpain 1, large subunit
18	Bos taurus	47.80	2	calpain 1, (mu/I) large subunit
19	synthetic construct	47.40	1	Homo sapiens calpain 1, (mu/I) large subunit
20	Pongo pygmaeus	47.40	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
21	Sus scrofa	46.20	1	calpain 1, large subunit
22	Gallus gallus	42.00	1	mu-calpain large subunit
23	Canis familiaris	37.40	1	PREDICTED: similar to Calpain-11 (Calcium-activat
24	Danio rerio	36.20	1	calpain 1, large subunit

organism	matching
N/A	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40
Rattus norvegicus	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40
Mus musculus	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|\|\|\|\|+\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAIKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40
Homo sapiens	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|\|\|\|\| \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| Sbjct 1 MAGIAAKLAK#DREAAEGLGSHERAIKYLNQDYEALRNECL 40
Homo sapiens	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|\|\|\|\| \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| Sbjct 1 MAGIAAKLAK#DREAAEGLGSHERAIKYLNQDYEALRNECL 40
Macaca mulatta	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|\|\|\|\| \|\| \|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| Sbjct 1 MAGIAAKLVK#DREAAEGLGSHERAIKYLNQDYEALRNECL 40
Xenopus tropicalis	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|+\|+\| +\|\|\|#+\| \| \|\|\|++\|+\|+ \|\|+\|\|\|\| \|\|\|\|\|\| Sbjct 1 MSGVADRLAK#ERALASGLGTNEKALPYLSQDYEALRNECL 40
Xenopus laevis	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|+\|\|\| \|+\|\|#\|\| \|+\|\|\|++++ +\|\|\|\|\|\|\|\| \|+ \|\| Sbjct 1 MSGIASKVAK#DRVLAQGLGTNDKPLKYLNQDYEQLKARCL 40
Gallus gallus	Query 2 AGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|\|\| +\| +#\|\| \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ \|\|+ Sbjct 5 GGIAARLQR#DRLRAEGVGEHNNAVKYLNQDYEALKQECI 43
Canis familiaris	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|+ +\| ++\|\|# + \|\| \|\|\|\|\|++\|+\|\|\| \|\|+\| \|\| +\|\| Sbjct 94 MSALAARIAK#QQAAAGGLGSHQKAVKYLGQDFEKLRQQCL 133
Danio rerio	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|+\|+\| \|\|\|# \| \| \| \|++ \|+\|\|\|\|\|++\|\|\|\|+\|\|\| Sbjct 1 MSGVASTLAK#KRALAAGFGTNSNAVKYLNQNFETLRSECL 40
Coturnix coturnix	Query 2 AGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|\|\| +\| +#\|\| \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ \|+ Sbjct 5 GGIAARLQR#DRLRAEGVGEHNNAVKYLNQDYEALKQACI 43
Stizostedion vitreum vitreum	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|+\|\|\| \| +# \|\| +\|+\|\| +\|+\|\|\|\|\|\|\|\| \|\| \|\| Sbjct 1 MSGIAATLQR#RREKEQGIGSCSQAVKYLNQDYEALRRRCL 40
Oncorhynchus mykiss	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|+\|+\| \|\|\|# \| \| \| \|++ \|+\|\|\|\|\|\|+\| \|\| +\|\| Sbjct 1 MSGMASNLAK#KRALAAGFGTNANAVKYLNQDFEALRAQCL 40
Xenopus laevis	Query 2 AGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|\|\| \|\| #\|\| \| \|+\|\|\|+ \|+\|\| \|\|\|\|\|+\|+ +\|+ Sbjct 5 GGIASKLKI#DRLKASGVGSHDCAVKYQNQDYESLKQQCV 43
Tetraodon nigroviridis	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|+\|\|\| \|\| # \|+ \|+\| \|+ +\| \|\|\|\|\|\|+\|+\|\| \|\| Sbjct 1 MSGIAAKLQH#HRDRAQGFGTASQAQKYLNQDFESLRESCL 40
Rattus norvegicus	Query 3 GIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|++ ++ \|# \|+ \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\| Sbjct 13 GVSAQVQK#QRDKELGLGRHENAIKYLGQDYENLRARCL 50
Bos taurus	Query 3 GIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|++ ++ \|# \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\| Sbjct 13 GVSAQVQK#QRAKELGLGRHENAIKYLGQDYEQLRVHCL 50
synthetic construct	Query 3 GIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|++ ++ \|# \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\| Sbjct 13 GVSAQVQK#QRARELGLGRHENAIKYLGQDYEQLRVRCL 50
Pongo pygmaeus	Query 3 GIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|++ ++ \|# \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\| Sbjct 13 GVSAQVQK#QRARELGLGRHENAIKYLGQDYEQLRVRCL 50
Sus scrofa	Query 3 GIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|++ ++ \|# \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\| Sbjct 13 GVSAQVQK#LRAKELGLGRHENAIKYLGQDYEQLRAHCL 50
Gallus gallus	Query 3 GIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 \|++ ++ +# \| \| \|\|\| \|+ \|+++ \|\|\| \|\|++\|\| Sbjct 13 GVSAQVQR#QRAKALGLGQHQNAVRFRGQDYAALRDDCL 50
Canis familiaris	Query 1 MAGIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNECL 40 + \|+\| ++ # \| \|+\|\|\| \| \| \| \|\|++\| \|+ \|\| Sbjct 83 LEGMAAHISH#SRLKAKGLGQHHNAQNYNNQNFEDLQAACL 122
Danio rerio	Query 3 GIAMKLAK#DREAAEGLGSHERAIKYLNQDYETLRNE 38 \|+\| +\| # + \|\|\| + \|+\|+\| \|\|\|\|\|\|\| + Sbjct 8 GMAARLRS#QWDRDAGLGQNHNAVKFLGQDYETLRAQ 43

[Site 7] AGIAMKLAKD¹¹-REAAEGLGSH

Asp¹¹ Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ala²	Gly³	Ile⁴	Ala⁵	Met⁶	Lys⁷	Leu⁸	Ala⁹	Lys¹⁰	Asp¹¹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg¹²	Glu¹³	Ala¹⁴	Ala¹⁵	Glu¹⁶	Gly¹⁷	Leu¹⁸	Gly¹⁹	Ser²⁰	His²¹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAMKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLE

#	organism	max score	hits	top seq
1	N/A	87.00	12	A Chain A, Crystal Structure Of A Mu-Like Calpain
2	Rattus norvegicus	86.30	5	calpain 2
3	Mus musculus	84.30	14	m-calpain
4	Homo sapiens	82.40	6	AF261089_1 calpain large polypeptide L2
5	Homo sapiens	82.40	2	calpain 2, large subunit
6	Macaca mulatta	80.90	2	PREDICTED: calpain 2, large subunit
7	Xenopus tropicalis	59.70	4	calpain 2, (m/II) large subunit
8	Xenopus laevis	58.20	4	calpain 8
9	Gallus gallus	57.40	2	calpain 1, (mu/I) large subunit
10	Canis familiaris	55.80	3	PREDICTED: similar to calpain 8
11	Coturnix coturnix	55.10	1	quail calpain
12	Danio rerio	54.30	6	calpain 2, (m/II) large subunit a
13	Stizostedion vitreum vitreum	54.30	1	putative calpain-like protein
14	Xenopus laevis	52.40	1	mu/m-calpain large subunit
15	Tetraodon nigroviridis	51.60	7	unnamed protein product
16	Oncorhynchus mykiss	51.60	1	m-calpain
17	Rattus norvegicus	49.70	1	calpain 1, large subunit
18	Bos taurus	48.10	2	micromolar calcium activated neutral protease 1
19	synthetic construct	47.80	1	Homo sapiens calpain 1, (mu/I) large subunit
20	Pongo pygmaeus	47.80	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
21	Sus scrofa	47.00	1	calpain 1, large subunit
22	Gallus gallus	41.60	1	mu-calpain large subunit
23	Canis familiaris	37.40	1	PREDICTED: similar to Calpain-11 (Calcium-activat
24	Danio rerio	36.20	1	calpain 1, large subunit

organism	matching
N/A	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41
Rattus norvegicus	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41
Mus musculus	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAIKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41
Homo sapiens	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|\|\|\|\| \|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKD#REAAEGLGSHERAIKYLNQDYEALRNECLE 41
Homo sapiens	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|\|\|\|\| \|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKD#REAAEGLGSHERAIKYLNQDYEALRNECLE 41
Macaca mulatta	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|\|\|\|\| \|\| \|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 1 MAGIAAKLVKD#REAAEGLGSHERAIKYLNQDYEALRNECLE 41
Xenopus tropicalis	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|+\|+\| +\|\|\|+#\| \| \|\|\|++\|+\|+ \|\|+\|\|\|\| \|\|\|\|\|\|+ Sbjct 1 MSGVADRLAKE#RALASGLGTNEKALPYLSQDYEALRNECLQ 41
Xenopus laevis	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|+\|\|\| \|+\|\|\|#\| \|+\|\|\|++++ +\|\|\|\|\|\|\|\| \|+ \|\|\| Sbjct 1 MSGIASKVAKD#RVLAQGLGTNDKPLKYLNQDYEQLKARCLE 41
Gallus gallus	Query 2 AGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|\|\| +\| +\|#\| \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ \|\|+\| Sbjct 5 GGIAARLQRD#RLRAEGVGEHNNAVKYLNQDYEALKQECIE 44
Canis familiaris	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|+ +\| ++\|\| #+ \|\| \|\|\|\|\|++\|+\|\|\| \|\|+\| \|\| +\|\|+ Sbjct 94 MSALAARIAKQ#QAAAGGLGSHQKAVKYLGQDFEKLRQQCLD 134
Coturnix coturnix	Query 2 AGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|\|\| +\| +\|#\| \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ \|+\| Sbjct 5 GGIAARLQRD#RLRAEGVGEHNNAVKYLNQDYEALKQACIE 44
Danio rerio	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECL 40 \|+\|+\| \|\|\| #\| \| \| \|++ \|+\|\|\|\|\|++\|\|\|\|+\|\|\| Sbjct 1 MSGVASTLAKK#RALAAGFGTNSNAVKYLNQNFETLRSECL 40
Stizostedion vitreum vitreum	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|+\|\|\| \| + #\|\| +\|+\|\| +\|+\|\|\|\|\|\|\|\| \|\| \|\|\| Sbjct 1 MSGIAATLQRR#REKEQGIGSCSQAVKYLNQDYEALRRRCLE 41
Xenopus laevis	Query 2 AGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|\|\| \|\| \|#\| \| \|+\|\|\|+ \|+\|\| \|\|\|\|\|+\|+ +\|+\| Sbjct 5 GGIASKLKID#RLKASGVGSHDCAVKYQNQDYESLKQQCVE 44
Tetraodon nigroviridis	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|+\|\|\| \|\| #\|+ \|+\| \|+ +\| \|\|\|\|\|\|+\|+\|\| \|\|\| Sbjct 1 MSGIAAKLQHH#RDRAQGFGTASQAQKYLNQDFESLRESCLE 41
Oncorhynchus mykiss	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECL 40 \|+\|+\| \|\|\| #\| \| \| \|++ \|+\|\|\|\|\|\|+\| \|\| +\|\| Sbjct 1 MSGMASNLAKK#RALAAGFGTNANAVKYLNQDFEALRAQCL 40
Rattus norvegicus	Query 3 GIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|++ ++ \| #\|+ \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|+ Sbjct 13 GVSAQVQKQ#RDKELGLGRHENAIKYLGQDYENLRARCLQ 51
Bos taurus	Query 3 GIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|++ ++ \| #\| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|+ Sbjct 13 GVSAQVQKQ#RAKELGLGRHENAIKYLGQDYEQLRVHCLQ 51
synthetic construct	Query 3 GIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|++ ++ \| #\| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|+ Sbjct 13 GVSAQVQKQ#RARELGLGRHENAIKYLGQDYEQLRVRCLQ 51
Pongo pygmaeus	Query 3 GIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|++ ++ \| #\| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|+ Sbjct 13 GVSAQVQKQ#RARELGLGRHENAIKYLGQDYEQLRVRCLQ 51
Sus scrofa	Query 3 GIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|++ ++ \| #\| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|+ Sbjct 13 GVSAQVQKL#RAKELGLGRHENAIKYLGQDYEQLRAHCLQ 51
Gallus gallus	Query 3 GIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECL 40 \|++ ++ + #\| \| \|\|\| \|+ \|+++ \|\|\| \|\|++\|\| Sbjct 13 GVSAQVQRQ#RAKALGLGQHQNAVRFRGQDYAALRDDCL 50
Canis familiaris	Query 1 MAGIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNECL 40 + \|+\| ++ #\| \|+\|\|\| \| \| \| \|\|++\| \|+ \|\| Sbjct 83 LEGMAAHISHS#RLKAKGLGQHHNAQNYNNQNFEDLQAACL 122
Danio rerio	Query 3 GIAMKLAKD#REAAEGLGSHERAIKYLNQDYETLRNE 38 \|+\| +\| # + \|\|\| + \|+\|+\| \|\|\|\|\|\|\| + Sbjct 8 GMAARLRSQ#WDRDAGLGQNHNAVKFLGQDYETLRAQ 43

[Site 8] GIAMKLAKDR¹²-EAAEGLGSHE

Arg¹² Glu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Gly³	Ile⁴	Ala⁵	Met⁶	Lys⁷	Leu⁸	Ala⁹	Lys¹⁰	Asp¹¹	Arg¹²

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Glu¹³	Ala¹⁴	Ala¹⁵	Glu¹⁶	Gly¹⁷	Leu¹⁸	Gly¹⁹	Ser²⁰	His²¹	Glu²²

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAMKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEA

#	organism	max score	hits	top seq
1	N/A	89.00	12	A Chain A, Crystal Structure Of A Mu-Like Calpain
2	Rattus norvegicus	87.80	5	calpain 2
3	Mus musculus	86.30	14	calpain 2
4	Homo sapiens	84.30	6	AF261089_1 calpain large polypeptide L2
5	Homo sapiens	84.30	2	calpain 2, large subunit
6	Macaca mulatta	82.80	2	PREDICTED: calpain 2, large subunit
7	Xenopus tropicalis	60.10	4	calpain 2, (m/II) large subunit
8	Xenopus laevis	58.90	4	calpain 8
9	Gallus gallus	57.80	2	calpain 1, (mu/I) large subunit
10	Canis familiaris	57.40	3	PREDICTED: similar to calpain 8
11	Coturnix coturnix	55.50	1	quail calpain
12	Stizostedion vitreum vitreum	54.70	1	putative calpain-like protein
13	Danio rerio	54.30	6	calpain 2, (m/II) large subunit a
14	Xenopus laevis	53.10	1	mu/m-calpain large subunit
15	Tetraodon nigroviridis	51.60	7	unnamed protein product
16	Oncorhynchus mykiss	51.60	1	m-calpain
17	Rattus norvegicus	49.70	1	calpain 1, large subunit
18	Bos taurus	48.50	2	micromolar calcium activated neutral protease 1
19	synthetic construct	48.50	1	Homo sapiens calpain 1, (mu/I) large subunit
20	Pongo pygmaeus	48.10	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
21	Sus scrofa	47.40	1	calpain 1, large subunit
22	Gallus gallus	42.40	1	mu-calpain large subunit
23	Canis familiaris	37.40	1	PREDICTED: similar to Calpain-11 (Calcium-activat
24	Danio rerio	36.20	1	calpain 1, large subunit

organism	matching
N/A	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42
Rattus norvegicus	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42
Mus musculus	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|\|\|\|\|+\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAIKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42
Homo sapiens	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|\|\|\|\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKDR#EAAEGLGSHERAIKYLNQDYEALRNECLEA 42
Homo sapiens	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|\|\|\|\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKDR#EAAEGLGSHERAIKYLNQDYEALRNECLEA 42
Macaca mulatta	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|\|\|\|\| \|\| \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLVKDR#EAAEGLGSHERAIKYLNQDYEALRNECLEA 42
Xenopus tropicalis	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|+\|+\| +\|\|\|+\|# \| \|\|\|++\|+\|+ \|\|+\|\|\|\| \|\|\|\|\|\|++ Sbjct 1 MSGVADRLAKER#ALASGLGTNEKALPYLSQDYEALRNECLQS 42
Xenopus laevis	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|+\|\|\| \|+\|\|\|\|# \|+\|\|\|++++ +\|\|\|\|\|\|\|\| \|+ \|\|\|+ Sbjct 1 MSGIASKVAKDR#VLAQGLGTNDKPLKYLNQDYEQLKARCLES 42
Gallus gallus	Query 2 AGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|\|\| +\| +\|\|# \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ \|\|+\|+ Sbjct 5 GGIAARLQRDR#LRAEGVGEHNNAVKYLNQDYEALKQECIES 45
Canis familiaris	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|+ +\| ++\|\| +# \|\| \|\|\|\|\|++\|+\|\|\| \|\|+\| \|\| +\|\|+\| Sbjct 94 MSALAARIAKQQ#AAAGGLGSHQKAVKYLGQDFEKLRQQCLDA 135
Coturnix coturnix	Query 2 AGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|\|\| +\| +\|\|# \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ \|+\|+ Sbjct 5 GGIAARLQRDR#LRAEGVGEHNNAVKYLNQDYEALKQACIES 45
Stizostedion vitreum vitreum	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|+\|\|\| \| + \|#\| +\|+\|\| +\|+\|\|\|\|\|\|\|\| \|\| \|\|\|+ Sbjct 1 MSGIAATLQRRR#EKEQGIGSCSQAVKYLNQDYEALRRRCLES 42
Danio rerio	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECL 40 \|+\|+\| \|\|\| \|# \| \| \|++ \|+\|\|\|\|\|++\|\|\|\|+\|\|\| Sbjct 1 MSGVASTLAKKR#ALAAGFGTNSNAVKYLNQNFETLRSECL 40
Xenopus laevis	Query 2 AGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|\|\| \|\| \|\|# \| \|+\|\|\|+ \|+\|\| \|\|\|\|\|+\|+ +\|+\|+ Sbjct 5 GGIASKLKIDR#LKASGVGSHDCAVKYQNQDYESLKQQCVES 45
Tetraodon nigroviridis	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|+\|\|\| \|\| \|#+ \|+\| \|+ +\| \|\|\|\|\|\|+\|+\|\| \|\|\| Sbjct 1 MSGIAAKLQHHR#DRAQGFGTASQAQKYLNQDFESLRESCLE 41
Oncorhynchus mykiss	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECL 40 \|+\|+\| \|\|\| \|# \| \| \|++ \|+\|\|\|\|\|\|+\| \|\| +\|\| Sbjct 1 MSGMASNLAKKR#ALAAGFGTNANAVKYLNQDFEALRAQCL 40
Rattus norvegicus	Query 3 GIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|++ ++ \| \|#+ \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|+ Sbjct 13 GVSAQVQKQR#DKELGLGRHENAIKYLGQDYENLRARCLQ 51
Bos taurus	Query 3 GIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLE 41 \|++ ++ \| \|# \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|+ Sbjct 13 GVSAQVQKQR#AKELGLGRHENAIKYLGQDYEQLRVHCLQ 51
synthetic construct	Query 3 GIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|++ ++ \| \|# \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++ Sbjct 13 GVSAQVQKQR#ARELGLGRHENAIKYLGQDYEQLRVRCLQS 52
Pongo pygmaeus	Query 3 GIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|++ ++ \| \|# \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++ Sbjct 13 GVSAQVQKQR#ARELGLGRHENAIKYLGQDYEQLRVRCLQS 52
Sus scrofa	Query 3 GIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|++ ++ \| \|# \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++ Sbjct 13 GVSAQVQKLR#AKELGLGRHENAIKYLGQDYEQLRAHCLQS 52
Gallus gallus	Query 3 GIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|++ ++ + \|# \| \|\|\| \|+ \|+++ \|\|\| \|\|++\|\| + Sbjct 13 GVSAQVQRQR#AKALGLGQHQNAVRFRGQDYAALRDDCLRS 52
Canis familiaris	Query 1 MAGIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNECL 40 + \|+\| ++ \|# \|+\|\|\| \| \| \| \|\|++\| \|+ \|\| Sbjct 83 LEGMAAHISHSR#LKAKGLGQHHNAQNYNNQNFEDLQAACL 122
Danio rerio	Query 3 GIAMKLAKDR#EAAEGLGSHERAIKYLNQDYETLRNE 38 \|+\| +\| #+ \|\|\| + \|+\|+\| \|\|\|\|\|\|\| + Sbjct 8 GMAARLRSQW#DRDAGLGQNHNAVKFLGQDYETLRAQ 43

[Site 9] IAMKLAKDRE¹³-AAEGLGSHER

Glu¹³ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ile⁴	Ala⁵	Met⁶	Lys⁷	Leu⁸	Ala⁹	Lys¹⁰	Asp¹¹	Arg¹²	Glu¹³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala¹⁴	Ala¹⁵	Glu¹⁶	Gly¹⁷	Leu¹⁸	Gly¹⁹	Ser²⁰	His²¹	Glu²²	Arg²³

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAMKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEAG

#	organism	max score	hits	top seq
1	N/A	91.70	12	A Chain A, Crystal Structure Of A Mu-Like Calpain
2	Rattus norvegicus	90.10	5	calpain 2
3	Mus musculus	88.60	14	calpain 2
4	Homo sapiens	86.70	6	AF261089_1 calpain large polypeptide L2
5	Homo sapiens	86.70	2	calpain 2, large subunit
6	Macaca mulatta	85.10	2	PREDICTED: calpain 2, large subunit
7	Xenopus tropicalis	62.40	4	calpain 2, (m/II) large subunit
8	Gallus gallus	60.10	2	calpain 1, (mu/I) large subunit
9	Xenopus laevis	59.30	4	calpain 8
10	Canis familiaris	59.30	3	PREDICTED: similar to calpain 8
11	Coturnix coturnix	57.80	1	quail calpain
12	Danio rerio	55.80	6	calpain 2, (m/II) large subunit a
13	Xenopus laevis	55.50	1	mu/m-calpain large subunit
14	Stizostedion vitreum vitreum	54.70	1	putative calpain-like protein
15	Tetraodon nigroviridis	53.50	7	unnamed protein product
16	Oncorhynchus mykiss	53.10	1	m-calpain
17	Rattus norvegicus	51.60	1	calpain 1, large subunit
18	synthetic construct	50.80	1	Homo sapiens calpain 1, (mu/I) large subunit
19	Pongo pygmaeus	50.80	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
20	Bos taurus	50.10	2	micromolar calcium activated neutral protease 1
21	Sus scrofa	49.70	1	calpain 1, large subunit
22	Gallus gallus	44.70	1	mu-calpain large subunit
23	Canis familiaris	38.90	1	PREDICTED: similar to Calpain-11 (Calcium-activat
24	Danio rerio	36.20	1	calpain 1, large subunit

organism	matching
N/A	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43
Rattus norvegicus	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43
Mus musculus	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\|\|\|+\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAIKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43
Homo sapiens	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\|\|\| \|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKDRE#AAEGLGSHERAIKYLNQDYEALRNECLEAG 43
Homo sapiens	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\|\|\| \|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKDRE#AAEGLGSHERAIKYLNQDYEALRNECLEAG 43
Macaca mulatta	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\|\|\| \|\| \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLVKDRE#AAEGLGSHERAIKYLNQDYEALRNECLEAG 43
Xenopus tropicalis	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|+\|+\| +\|\|\|+\| # \| \|\|\|++\|+\|+ \|\|+\|\|\|\| \|\|\|\|\|\|++\| Sbjct 1 MSGVADRLAKERA#LASGLGTNEKALPYLSQDYEALRNECLQSG 43
Gallus gallus	Query 2 AGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\| +\| +\|\| # \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ \|\|+\|+\| Sbjct 5 GGIAARLQRDRL#RAEGVGEHNNAVKYLNQDYEALKQECIESG 46
Xenopus laevis	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|+\|\|\| \|+\|\|\|\| # \|+\|\|\|++++ +\|\|\|\|\|\|\|\| \|+ \|\|\|+ Sbjct 1 MSGIASKVAKDRV#LAQGLGTNDKPLKYLNQDYEQLKARCLES 42
Canis familiaris	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|+ +\| ++\|\| + #\|\| \|\|\|\|\|++\|+\|\|\| \|\|+\| \|\| +\|\|+\|\| Sbjct 94 MSALAARIAKQQA#AAGGLGSHQKAVKYLGQDFEKLRQQCLDAG 136
Coturnix coturnix	Query 2 AGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\| +\| +\|\| # \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ \|+\|+\| Sbjct 5 GGIAARLQRDRL#RAEGVGEHNNAVKYLNQDYEALKQACIESG 46
Danio rerio	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|+\|+\| \|\|\| \| # \| \| \|++ \|+\|\|\|\|\|++\|\|\|\|+\|\|\| \| Sbjct 1 MSGVASTLAKKRA#LAAGFGTNSNAVKYLNQNFETLRSECLSRG 43
Xenopus laevis	Query 2 AGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\| \|\| \|\| # \| \|+\|\|\|+ \|+\|\| \|\|\|\|\|+\|+ +\|+\|+\| Sbjct 5 GGIASKLKIDRL#KASGVGSHDCAVKYQNQDYESLKQQCVESG 46
Stizostedion vitreum vitreum	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEA 42 \|+\|\|\| \| + \|\|# +\|+\|\| +\|+\|\|\|\|\|\|\|\| \|\| \|\|\|+ Sbjct 1 MSGIAATLQRRRE#KEQGIGSCSQAVKYLNQDYEALRRRCLES 42
Tetraodon nigroviridis	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|+\|\|\| \|\| \|+# \|+\| \|+ +\| \|\|\|\|\|\|+\|+\|\| \|\|\| \| Sbjct 1 MSGIAAKLQHHRD#RAQGFGTASQAQKYLNQDFESLRESCLERG 43
Oncorhynchus mykiss	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|+\|+\| \|\|\| \| # \| \| \|++ \|+\|\|\|\|\|\|+\| \|\| +\|\| \| Sbjct 1 MSGMASNLAKKRA#LAAGFGTNANAVKYLNQDFEALRAQCLNRG 43
Rattus norvegicus	Query 3 GIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|++ ++ \| \|+# \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|+ \| Sbjct 13 GVSAQVQKQRD#KELGLGRHENAIKYLGQDYENLRARCLQNG 53
synthetic construct	Query 3 GIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|++ ++ \| \| # \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++\| Sbjct 13 GVSAQVQKQRA#RELGLGRHENAIKYLGQDYEQLRVRCLQSG 53
Pongo pygmaeus	Query 3 GIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|++ ++ \| \| # \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++\| Sbjct 13 GVSAQVQKQRA#RELGLGRHENAIKYLGQDYEQLRVRCLQSG 53
Bos taurus	Query 3 GIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|++ ++ \| \| # \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|+ \| Sbjct 13 GVSAQVQKQRA#KELGLGRHENAIKYLGQDYEQLRVHCLQRG 53
Sus scrofa	Query 3 GIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|++ ++ \| \| # \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++\| Sbjct 13 GVSAQVQKLRA#KELGLGRHENAIKYLGQDYEQLRAHCLQSG 53
Gallus gallus	Query 3 GIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|++ ++ + \| # \| \|\|\| \|+ \|+++ \|\|\| \|\|++\|\| +\| Sbjct 13 GVSAQVQRQRA#KALGLGQHQNAVRFRGQDYAALRDDCLRSG 53
Canis familiaris	Query 1 MAGIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNECLEAG 43 + \|+\| ++ \| # \|+\|\|\| \| \| \| \|\|++\| \|+ \|\| \| Sbjct 83 LEGMAAHISHSRL#KAKGLGQHHNAQNYNNQNFEDLQAACLRKG 125
Danio rerio	Query 3 GIAMKLAKDRE#AAEGLGSHERAIKYLNQDYETLRNE 38 \|+\| +\| +# \|\|\| + \|+\|+\| \|\|\|\|\|\|\| + Sbjct 8 GMAARLRSQWD#RDAGLGQNHNAVKFLGQDYETLRAQ 43

[Site 10] AMKLAKDREA¹⁴-AEGLGSHERA

Ala¹⁴ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ala⁵	Met⁶	Lys⁷	Leu⁸	Ala⁹	Lys¹⁰	Asp¹¹	Arg¹²	Glu¹³	Ala¹⁴

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala¹⁵	Glu¹⁶	Gly¹⁷	Leu¹⁸	Gly¹⁹	Ser²⁰	His²¹	Glu²²	Arg²³	Ala²⁴

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAMKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEAGA

#	organism	max score	hits	top seq
1	N/A	93.60	12	A Chain A, Crystal Structure Of A Mu-Like Calpain
2	Rattus norvegicus	92.00	5	calpain 2
3	Mus musculus	90.50	14	calpain 2
4	Homo sapiens	87.00	2	calpain 2, large subunit
5	Homo sapiens	86.70	6	AF261089_1 calpain large polypeptide L2
6	Macaca mulatta	85.50	2	PREDICTED: calpain 2, large subunit
7	Xenopus tropicalis	62.80	4	calpain 2, (m/II) large subunit
8	Gallus gallus	60.10	2	calpain 1, (mu/I) large subunit
9	Xenopus laevis	59.30	4	calpain 8
10	Canis familiaris	59.30	3	PREDICTED: similar to calpain 8
11	Coturnix coturnix	59.30	1	quail calpain
12	Danio rerio	55.80	6	calpain 2, (m/II) large subunit a
13	Xenopus laevis	55.50	2	mu/m-calpain large subunit
14	Stizostedion vitreum vitreum	54.70	1	putative calpain-like protein
15	Tetraodon nigroviridis	53.50	7	unnamed protein product
16	Oncorhynchus mykiss	53.10	1	m-calpain
17	Bos taurus	52.00	2	micromolar calcium activated neutral protease 1
18	Rattus norvegicus	52.00	1	calpain 1, large subunit
19	synthetic construct	51.20	1	Homo sapiens calpain 1, (mu/I) large subunit
20	Pongo pygmaeus	50.80	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
21	Sus scrofa	50.10	1	calpain 1, large subunit
22	Gallus gallus	45.10	1	mu-calpain large subunit
23	Canis familiaris	38.90	1	PREDICTED: similar to Calpain-11 (Calcium-activat
24	Danio rerio	36.20	1	calpain 1, large subunit

organism	matching
N/A	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAGA 44 \|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAGA 44
Rattus norvegicus	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAGA 44 \|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAGA 44
Mus musculus	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAGA 44 \|\|\|\|\|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAIKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAGA 44
Homo sapiens	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\|\|\| \|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKDREA#AEGLGSHERAIKYLNQDYEALRNECLEAG 43
Homo sapiens	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\|\|\| \|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKDREA#AEGLGSHERAIKYLNQDYEALRNECLEAG 43
Macaca mulatta	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\|\|\| \|\| \|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLVKDREA#AEGLGSHERAIKYLNQDYEALRNECLEAG 43
Xenopus tropicalis	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|+\|+\| +\|\|\|+\| #\| \|\|\|++\|+\|+ \|\|+\|\|\|\| \|\|\|\|\|\|++\| Sbjct 1 MSGVADRLAKERAL#ASGLGTNEKALPYLSQDYEALRNECLQSG 43
Gallus gallus	Query 2 AGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\| +\| +\|\| #\|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ \|\|+\|+\| Sbjct 5 GGIAARLQRDRLR#AEGVGEHNNAVKYLNQDYEALKQECIESG 46
Xenopus laevis	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEA 42 \|+\|\|\| \|+\|\|\|\| #\|+\|\|\|++++ +\|\|\|\|\|\|\|\| \|+ \|\|\|+ Sbjct 1 MSGIASKVAKDRVL#AQGLGTNDKPLKYLNQDYEQLKARCLES 42
Canis familiaris	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|+ +\| ++\|\| + \|#\| \|\|\|\|\|++\|+\|\|\| \|\|+\| \|\| +\|\|+\|\| Sbjct 94 MSALAARIAKQQAA#AGGLGSHQKAVKYLGQDFEKLRQQCLDAG 136
Coturnix coturnix	Query 2 AGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAGA 44 \|\|\| +\| +\|\| #\|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ \|+\|+\|\| Sbjct 5 GGIAARLQRDRLR#AEGVGEHNNAVKYLNQDYEALKQACIESGA 47
Danio rerio	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|+\|+\| \|\|\| \| #\| \| \|++ \|+\|\|\|\|\|++\|\|\|\|+\|\|\| \| Sbjct 1 MSGVASTLAKKRAL#AAGFGTNSNAVKYLNQNFETLRSECLSRG 43
Xenopus laevis	Query 2 AGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|\|\| \|\| \|\| #\| \|+\|\|\|+ \|+\|\| \|\|\|\|\|+\|+ +\|+\|+\| Sbjct 5 GGIASKLKIDRLK#ASGVGSHDCAVKYQNQDYESLKQQCVESG 46
Stizostedion vitreum vitreum	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEA 42 \|+\|\|\| \| + \|\| # +\|+\|\| +\|+\|\|\|\|\|\|\|\| \|\| \|\|\|+ Sbjct 1 MSGIAATLQRRREK#EQGIGSCSQAVKYLNQDYEALRRRCLES 42
Tetraodon nigroviridis	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|+\|\|\| \|\| \|+ #\|+\| \|+ +\| \|\|\|\|\|\|+\|+\|\| \|\|\| \| Sbjct 1 MSGIAAKLQHHRDR#AQGFGTASQAQKYLNQDFESLRESCLERG 43
Oncorhynchus mykiss	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAGA 44 \|+\|+\| \|\|\| \| #\| \| \|++ \|+\|\|\|\|\|\|+\| \|\| +\|\| \| Sbjct 1 MSGMASNLAKKRAL#AAGFGTNANAVKYLNQDFEALRAQCLNRGG 44
Bos taurus	Query 3 GIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAGA 44 \|++ ++ \| \| # \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|+ \|\| Sbjct 13 GVSAQVQKQRAK#ELGLGRHENAIKYLGQDYEQLRVHCLQRGA 54
Rattus norvegicus	Query 3 GIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|++ ++ \| \|+ # \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|+ \| Sbjct 13 GVSAQVQKQRDK#ELGLGRHENAIKYLGQDYENLRARCLQNG 53
synthetic construct	Query 3 GIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|++ ++ \| \| # \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++\| Sbjct 13 GVSAQVQKQRAR#ELGLGRHENAIKYLGQDYEQLRVRCLQSG 53
Pongo pygmaeus	Query 3 GIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAG 43 \|++ ++ \| \| # \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++\| Sbjct 13 GVSAQVQKQRAR#ELGLGRHENAIKYLGQDYEQLRVRCLQSG 53
Sus scrofa	Query 3 GIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAGA 44 \|++ ++ \| \| # \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++\|+ Sbjct 13 GVSAQVQKLRAK#ELGLGRHENAIKYLGQDYEQLRAHCLQSGS 54
Gallus gallus	Query 3 GIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAGA 44 \|++ ++ + \| #\| \|\|\| \|+ \|+++ \|\|\| \|\|++\|\| +\|+ Sbjct 13 GVSAQVQRQRAK#ALGLGQHQNAVRFRGQDYAALRDDCLRSGS 54
Canis familiaris	Query 1 MAGIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNECLEAG 43 + \|+\| ++ \| #\|+\|\|\| \| \| \| \|\|++\| \|+ \|\| \| Sbjct 83 LEGMAAHISHSRLK#AKGLGQHHNAQNYNNQNFEDLQAACLRKG 125
Danio rerio	Query 3 GIAMKLAKDREA#AEGLGSHERAIKYLNQDYETLRNE 38 \|+\| +\| + # \|\|\| + \|+\|+\| \|\|\|\|\|\|\| + Sbjct 8 GMAARLRSQWDR#DAGLGQNHNAVKFLGQDYETLRAQ 43

[Site 11] AAEGLGSHER²³-AIKYLNQDYE

Arg²³ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ala¹⁴	Ala¹⁵	Glu¹⁶	Gly¹⁷	Leu¹⁸	Gly¹⁹	Ser²⁰	His²¹	Glu²²	Arg²³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala²⁴	Ile²⁵	Lys²⁶	Tyr²⁷	Leu²⁸	Asn²⁹	Gln³⁰	Asp³¹	Tyr³²	Glu³³

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAMKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEAGALFQDPSFPA

#	organism	max score	hits	top seq
1	N/A	112.00	12	A Chain A, Crystal Structure Of M-Calpain
2	Rattus norvegicus	111.00	10	calpain 2
3	Mus musculus	110.00	19	m-calpain large subunit
4	Homo sapiens	105.00	11	AF261089_1 calpain large polypeptide L2
5	Homo sapiens	105.00	2	calpain 2, large subunit
6	Macaca mulatta	104.00	2	PREDICTED: calpain 2, large subunit
7	Xenopus tropicalis	77.40	5	calpain 2, (m/II) large subunit
8	Gallus gallus	77.40	2	calpain 1, (mu/I) large subunit
9	Coturnix coturnix	76.30	1	quail calpain
10	Canis familiaris	75.10	5	PREDICTED: similar to calpain 8
11	Xenopus laevis	73.90	5	calpain 8
12	Xenopus laevis	72.40	2	mu/m-calpain large subunit
13	Danio rerio	71.60	6	calpain 2, (m/II) large subunit a
14	Oncorhynchus mykiss	70.50	1	m-calpain
15	Stizostedion vitreum vitreum	68.90	1	putative calpain-like protein
16	Rattus norvegicus	65.50	1	calpain 1, large subunit
17	Tetraodon nigroviridis	64.30	12	unnamed protein product
18	Bos taurus	63.90	6	calpain 1, (mu/I) large subunit
19	synthetic construct	63.20	1	Homo sapiens calpain 1, (mu/I) large subunit
20	Pongo pygmaeus	62.80	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
21	Sus scrofa	62.40	2	calpain 1, large subunit
22	Gallus gallus	57.40	1	mu-calpain large subunit
23	Canis familiaris	51.20	1	PREDICTED: similar to Calpain-11 (Calcium-activat
24	Danio rerio	48.10	1	calpain 1, large subunit
25	Drosophila melanogaster	42.00	4	CAA55297.1
26	Drosophila melanogaster	42.00	2	Calpain-A CG7563-PB, isoform B
27	Oryctolagus cuniculus	41.20	1	calpain 3, (p94)

organism	matching
N/A	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53
Rattus norvegicus	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53
Mus musculus	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAIKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53
Homo sapiens	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKDREAAEGLGSHER#AIKYLNQDYEALRNECLEAGTLFQDPSFPA 53
Homo sapiens	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKDREAAEGLGSHER#AIKYLNQDYEALRNECLEAGTLFQDPSFPA 53
Macaca mulatta	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|\|\|\|\| \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLVKDREAAEGLGSHER#AIKYLNQDYEALRNECLEAGTLFQDPSFPA 53
Xenopus tropicalis	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|+\|+\| +\|\|\|+\| \| \|\|\|++\|+#\|+ \|\|+\|\|\|\| \|\|\|\|\|\|++\| \|\|+\| \|\|\|\| Sbjct 1 MSGVADRLAKERALASGLGTNEK#ALPYLSQDYEALRNECLQSGTLFKDLSFPA 53
Gallus gallus	Query 2 AGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|\|\| +\| +\|\| \|\|\|+\| \| #\|+\|\|\|\|\|\|\|\| \|+ \|\|+\|+\| \|\|+\|\| \|\|\| Sbjct 5 GGIAARLQRDRLRAEGVGEHNN#AVKYLNQDYEALKQECIESGTLFRDPQFPA 56
Coturnix coturnix	Query 2 AGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|\|\| +\| +\|\| \|\|\|+\| \| #\|+\|\|\|\|\|\|\|\| \|+ \|+\|+\|\|\|\|+\|\| \|\|\| Sbjct 5 GGIAARLQRDRLRAEGVGEHNN#AVKYLNQDYEALKQACIESGALFRDPQFPA 56
Canis familiaris	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|+ +\| ++\|\| + \|\| \|\|\|\|\|++#\|+\|\|\| \|\|+\| \|\| +\|\|+\|\| \|\|+\|\| \|\|\| Sbjct 94 MSALAARIAKQQAAAGGLGSHQK#AVKYLGQDFEKLRQQCLDAGVLFKDPEFPA 146
Xenopus laevis	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|+\|\|\| \|+\|\|\|\| \|+\|\|\|++++# +\|\|\|\|\|\|\|\| \|+ \|\|\|+ \|\|+\| +\|\|\| Sbjct 1 MSGIASKVAKDRVLAQGLGTNDK#PLKYLNQDYEQLKARCLESNTLFEDETFPA 53
Xenopus laevis	Query 2 AGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|\|\| \|\| \|\| \| \|+\|\|\|+ #\|+\|\| \|\|\|\|\|+\|+ +\|+\|+\| \|\|+\|\|+\|\|\| Sbjct 5 GGIASKLKIDRLKASGVGSHDC#AVKYQNQDYESLKQQCVESGILFEDPNFPA 56
Danio rerio	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|+\|+\| \|\|\| \| \| \| \|++ #\|+\|\|\|\|\|++\|\|\|\|+\|\|\| \| \|\| \|\|+\|\|\| Sbjct 1 MSGVASTLAKKRALAAGFGTNSN#AVKYLNQNFETLRSECLSRGELFCDPAFPA 53
Oncorhynchus mykiss	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|+\|+\| \|\|\| \| \| \| \|++ #\|+\|\|\|\|\|\|+\| \|\| +\|\| \| \|\| \|\|+\|\|\| Sbjct 1 MSGMASNLAKKRALAAGFGTNAN#AVKYLNQDFEALRAQCLNRGGLFSDPTFPA 53
Stizostedion vitreum vitreum	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|+\|\|\| \| + \|\| +\|+\|\| +#\|+\|\|\|\|\|\|\|\| \|\| \|\|\|+ +\|\|+\| +\|\|\| Sbjct 1 MSGIAATLQRRREKEQGIGSCSQ#AVKYLNQDYEALRRRCLESESLFKDDAFPA 53
Rattus norvegicus	Query 3 GIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFP 52 \|++ ++ \| \|+ \|\|\| \|\| #\|\|\|\|\| \|\|\|\| \|\| \|\|+ \| \|\|\|\| +\|\| Sbjct 13 GVSAQVQKQRDKELGLGRHEN#AIKYLGQDYENLRARCLQNGVLFQDDAFP 62
Tetraodon nigroviridis	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|+\|\|\| \|\| \|+ \|+\| \|+ +#\| \|\|\|\|\|\|+\|+\|\| \|\|\| \| \|\|\|\| \| \| Sbjct 1 MSGIAAKLQHHRDRAQGFGTASQ#AQKYLNQDFESLRESCLERGELFQDEYFEA 53
Bos taurus	Query 3 GIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFP 52 \|++ ++ \| \| \|\|\| \|\| #\|\|\|\|\| \|\|\|\| \|\| \|\|+ \|\|\|\|+\| +\|\| Sbjct 13 GVSAQVQKQRAKELGLGRHEN#AIKYLGQDYEQLRVHCLQRGALFRDEAFP 62
synthetic construct	Query 3 GIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFP 52 \|++ ++ \| \| \|\|\| \|\| #\|\|\|\|\| \|\|\|\| \|\| \|\|++\| \|\|+\| +\|\| Sbjct 13 GVSAQVQKQRARELGLGRHEN#AIKYLGQDYEQLRVRCLQSGTLFRDEAFP 62
Pongo pygmaeus	Query 3 GIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFP 52 \|++ ++ \| \| \|\|\| \|\| #\|\|\|\|\| \|\|\|\| \|\| \|\|++\| \|\|+\| +\|\| Sbjct 13 GVSAQVQKQRARELGLGRHEN#AIKYLGQDYEQLRVRCLQSGTLFRDEAFP 62
Sus scrofa	Query 3 GIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFP 52 \|++ ++ \| \| \|\|\| \|\| #\|\|\|\|\| \|\|\|\| \|\| \|\|++\|+\|\|+\| +\|\| Sbjct 13 GVSAQVQKLRAKELGLGRHEN#AIKYLGQDYEQLRAHCLQSGSLFRDEAFP 62
Gallus gallus	Query 3 GIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFP 52 \|++ ++ + \| \| \|\|\| \|+ #\|+++ \|\|\| \|\|++\|\| +\|+\|\|+\| +\|\| Sbjct 13 GVSAQVQRQRAKALGLGQHQN#AVRFRGQDYAALRDDCLRSGSLFRDETFP 62
Canis familiaris	Query 1 MAGIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 + \|+\| ++ \| \|+\|\|\| \| #\| \| \|\|++\| \|+ \|\| \| \|\|+\| \|\|\| Sbjct 83 LEGMAAHISHSRLKAKGLGQHHN#AQNYNNQNFEDLQAACLRKGELFEDSFFPA 135
Danio rerio	Query 3 GIAMKLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 \|+\| +\| + \|\|\| + #\|+\|+\| \|\|\|\|\|\|\| + ++ \|\|+\|\| \| \| Sbjct 8 GMAARLRSQWDRDAGLGQNHN#AVKFLGQDYETLRAQSQQSRRLFEDPMFTA 58
Drosophila melanogaster	Query 30 QDYETLRNECLEAGALFQDPSFPA 53 \|\|\|\|\|+ \| \|\| +\|+\|\|+\|\| \|\|\| Sbjct 50 QDYETILNSCLASGSLFEDPLFPA 73
Drosophila melanogaster	Query 30 QDYETLRNECLEAGALFQDPSFPA 53 \|\|\|\|\|+ \| \|\| +\|+\|\|+\|\| \|\|\| Sbjct 73 QDYETILNSCLASGSLFEDPLFPA 96
Oryctolagus cuniculus	Query 7 KLAKDREAAEGLGSHER#AIKYLNQDYETLRNECLEAGALFQDPSFPA 53 ++ ++\|+ \| \|+ +# \|\|\| \|\|+ \|\| \|\| \|\|+\| \|\|\| Sbjct 12 RVIRERDRRNGEGTVTQ#PIKYEGQDFVVLRQRCLAQKCLFEDRVFPA 58

[Site 12] FPALPSSLGF⁶⁰-KELGPYSSKT

Phe⁶⁰ Lys

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Phe⁵¹	Pro⁵²	Ala⁵³	Leu⁵⁴	Pro⁵⁵	Ser⁵⁶	Ser⁵⁷	Leu⁵⁸	Gly⁵⁹	Phe⁶⁰

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Lys⁶¹	Glu⁶²	Leu⁶³	Gly⁶⁴	Pro⁶⁵	Tyr⁶⁶	Ser⁶⁷	Ser⁶⁸	Lys⁶⁹	Thr⁷⁰

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 13] IEWKRPTEIC⁸²-ADPQFIIGGA

Cys⁸² Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ile⁷³	Glu⁷⁴	Trp⁷⁵	Lys⁷⁶	Arg⁷⁷	Pro⁷⁸	Thr⁷⁹	Glu⁸⁰	Ile⁸¹	Cys⁸²

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala⁸³	Asp⁸⁴	Pro⁸⁵	Gln⁸⁶	Phe⁸⁷	Ile⁸⁸	Ile⁸⁹	Gly⁹⁰	Gly⁹¹	Ala⁹²

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 14] LLAAIASLTL¹¹⁶-NEEILARVVP

Leu¹¹⁶ Asn

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Leu¹⁰⁷	Leu¹⁰⁸	Ala¹⁰⁹	Ala¹¹⁰	Ile¹¹¹	Ala¹¹²	Ser¹¹³	Leu¹¹⁴	Thr¹¹⁵	Leu¹¹⁶

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Asn¹¹⁷	Glu¹¹⁸	Glu¹¹⁹	Ile¹²⁰	Leu¹²¹	Ala¹²²	Arg¹²³	Val¹²⁴	Val¹²⁵	Pro¹²⁶

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 15] YEALSGGATT²⁰¹-EGFEDFTGGI

Thr²⁰¹ Glu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Tyr¹⁹²	Glu¹⁹³	Ala¹⁹⁴	Leu¹⁹⁵	Ser¹⁹⁶	Gly¹⁹⁷	Gly¹⁹⁸	Ala¹⁹⁹	Thr²⁰⁰	Thr²⁰¹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Glu²⁰²	Gly²⁰³	Phe²⁰⁴	Glu²⁰⁵	Asp²⁰⁶	Phe²⁰⁷	Thr²⁰⁸	Gly²⁰⁹	Gly²¹⁰	Ile²¹¹

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 16] GATTEGFEDF²⁰⁷-TGGIAEWYEL

Phe²⁰⁷ Thr

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Gly¹⁹⁸	Ala¹⁹⁹	Thr²⁰⁰	Thr²⁰¹	Glu²⁰²	Gly²⁰³	Phe²⁰⁴	Glu²⁰⁵	Asp²⁰⁶	Phe²⁰⁷

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Thr²⁰⁸	Gly²⁰⁹	Gly²¹⁰	Ile²¹¹	Ala²¹²	Glu²¹³	Trp²¹⁴	Tyr²¹⁵	Glu²¹⁶	Leu²¹⁷

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 17] TTEGFEDFTG²⁰⁹-GIAEWYELRK

Gly²⁰⁹ Gly

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Thr²⁰⁰	Thr²⁰¹	Glu²⁰²	Gly²⁰³	Phe²⁰⁴	Glu²⁰⁵	Asp²⁰⁶	Phe²⁰⁷	Thr²⁰⁸	Gly²⁰⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gly²¹⁰	Ile²¹¹	Ala²¹²	Glu²¹³	Trp²¹⁴	Tyr²¹⁵	Glu²¹⁶	Leu²¹⁷	Arg²¹⁸	Lys²¹⁹

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 18] LGCSIDITSA²⁴⁷-ADSEAVTYQK

Ala²⁴⁷ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Leu²³⁸	Gly²³⁹	Cys²⁴⁰	Ser²⁴¹	Ile²⁴²	Asp²⁴³	Ile²⁴⁴	Thr²⁴⁵	Ser²⁴⁶	Ala²⁴⁷

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala²⁴⁸	Asp²⁴⁹	Ser²⁵⁰	Glu²⁵¹	Ala²⁵²	Val²⁵³	Thr²⁵⁴	Tyr²⁵⁵	Gln²⁵⁶	Lys²⁵⁷

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 19] CSIDITSAAD²⁴⁹-SEAVTYQKLV

Asp²⁴⁹ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Cys²⁴⁰	Ser²⁴¹	Ile²⁴²	Asp²⁴³	Ile²⁴⁴	Thr²⁴⁵	Ser²⁴⁶	Ala²⁴⁷	Ala²⁴⁸	Asp²⁴⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser²⁵⁰	Glu²⁵¹	Ala²⁵²	Val²⁵³	Thr²⁵⁴	Tyr²⁵⁵	Gln²⁵⁶	Lys²⁵⁷	Leu²⁵⁸	Val²⁵⁹

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 20] SAADSEAVTY²⁵⁵-QKLVKGHAYS

Tyr²⁵⁵ Gln

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ser²⁴⁶	Ala²⁴⁷	Ala²⁴⁸	Asp²⁴⁹	Ser²⁵⁰	Glu²⁵¹	Ala²⁵²	Val²⁵³	Thr²⁵⁴	Tyr²⁵⁵

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gln²⁵⁶	Lys²⁵⁷	Leu²⁵⁸	Val²⁵⁹	Lys²⁶⁰	Gly²⁶¹	His²⁶²	Ala²⁶³	Tyr²⁶⁴	Ser²⁶⁵

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 21] LVKGHAYSVT²⁶⁷-GAEEVESSGS

Thr²⁶⁷ Gly

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Leu²⁵⁸	Val²⁵⁹	Lys²⁶⁰	Gly²⁶¹	His²⁶²	Ala²⁶³	Tyr²⁶⁴	Ser²⁶⁵	Val²⁶⁶	Thr²⁶⁷

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gly²⁶⁸	Ala²⁶⁹	Glu²⁷⁰	Glu²⁷¹	Val²⁷²	Glu²⁷³	Ser²⁷⁴	Ser²⁷⁵	Gly²⁷⁶	Ser²⁷⁷

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 22] CDSYKKWKLT³⁵⁹-KMDGNWRRGS

Thr³⁵⁹ Lys

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Cys³⁵⁰	Asp³⁵¹	Ser³⁵²	Tyr³⁵³	Lys³⁵⁴	Lys³⁵⁵	Trp³⁵⁶	Lys³⁵⁷	Leu³⁵⁸	Thr³⁵⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Lys³⁶⁰	Met³⁶¹	Asp³⁶²	Gly³⁶³	Asn³⁶⁴	Trp³⁶⁵	Arg³⁶⁶	Arg³⁶⁷	Gly³⁶⁸	Ser³⁶⁹

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 23] DSYKKWKLTK³⁶⁰-MDGNWRRGST

Lys³⁶⁰ Met

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Asp³⁵¹	Ser³⁵²	Tyr³⁵³	Lys³⁵⁴	Lys³⁵⁵	Trp³⁵⁶	Lys³⁵⁷	Leu³⁵⁸	Thr³⁵⁹	Lys³⁶⁰

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Met³⁶¹	Asp³⁶²	Gly³⁶³	Asn³⁶⁴	Trp³⁶⁵	Arg³⁶⁶	Arg³⁶⁷	Gly³⁶⁸	Ser³⁶⁹	Thr³⁷⁰

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 24] GGCRNYPNTF³⁸¹-WMNPQYLIKL

Phe³⁸¹ Trp

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Gly³⁷²	Gly³⁷³	Cys³⁷⁴	Arg³⁷⁵	Asn³⁷⁶	Tyr³⁷⁷	Pro³⁷⁸	Asn³⁷⁹	Thr³⁸⁰	Phe³⁸¹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Trp³⁸²	Met³⁸³	Asn³⁸⁴	Pro³⁸⁵	Gln³⁸⁶	Tyr³⁸⁷	Leu³⁸⁸	Ile³⁸⁹	Lys³⁹⁰	Leu³⁹¹

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 25] CRNYPNTFWM³⁸³-NPQYLIKLEE

Met³⁸³ Asn

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Cys³⁷⁴	Arg³⁷⁵	Asn³⁷⁶	Tyr³⁷⁷	Pro³⁷⁸	Asn³⁷⁹	Thr³⁸⁰	Phe³⁸¹	Trp³⁸²	Met³⁸³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Asn³⁸⁴	Pro³⁸⁵	Gln³⁸⁶	Tyr³⁸⁷	Leu³⁸⁸	Ile³⁸⁹	Lys³⁹⁰	Leu³⁹¹	Glu³⁹²	Glu³⁹³

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 26] PNTFWMNPQY³⁸⁷-LIKLEEEDED

Tyr³⁸⁷ Leu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Pro³⁷⁸	Asn³⁷⁹	Thr³⁸⁰	Phe³⁸¹	Trp³⁸²	Met³⁸³	Asn³⁸⁴	Pro³⁸⁵	Gln³⁸⁶	Tyr³⁸⁷

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Leu³⁸⁸	Ile³⁸⁹	Lys³⁹⁰	Leu³⁹¹	Glu³⁹²	Glu³⁹³	Glu³⁹⁴	Asp³⁹⁵	Glu³⁹⁶	Asp³⁹⁷

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 27] FWMNPQYLIK³⁹⁰-LEEEDEDDED

Lys³⁹⁰ Leu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Phe³⁸¹	Trp³⁸²	Met³⁸³	Asn³⁸⁴	Pro³⁸⁵	Gln³⁸⁶	Tyr³⁸⁷	Leu³⁸⁸	Ile³⁸⁹	Lys³⁹⁰

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Leu³⁹¹	Glu³⁹²	Glu³⁹³	Glu³⁹⁴	Asp³⁹⁵	Glu³⁹⁶	Asp³⁹⁷	Asp³⁹⁸	Glu³⁹⁹	Asp⁴⁰⁰

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 28] MNPQYLIKLE³⁹²-EEDEDDEDGE

Glu³⁹² Glu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Met³⁸³	Asn³⁸⁴	Pro³⁸⁵	Gln³⁸⁶	Tyr³⁸⁷	Leu³⁸⁸	Ile³⁸⁹	Lys³⁹⁰	Leu³⁹¹	Glu³⁹²

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Glu³⁹³	Glu³⁹⁴	Asp³⁹⁵	Glu³⁹⁶	Asp³⁹⁷	Asp³⁹⁸	Glu³⁹⁹	Asp⁴⁰⁰	Gly⁴⁰¹	Glu⁴⁰²

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 29] DEDGERGCTF⁴⁰⁷-LVGLIQKHRR

Phe⁴⁰⁷ Leu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Asp³⁹⁸	Glu³⁹⁹	Asp⁴⁰⁰	Gly⁴⁰¹	Glu⁴⁰²	Arg⁴⁰³	Gly⁴⁰⁴	Cys⁴⁰⁵	Thr⁴⁰⁶	Phe⁴⁰⁷

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Leu⁴⁰⁸	Val⁴⁰⁹	Gly⁴¹⁰	Leu⁴¹¹	Ile⁴¹²	Gln⁴¹³	Lys⁴¹⁴	His⁴¹⁵	Arg⁴¹⁶	Arg⁴¹⁷

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 30] DGERGCTFLV⁴⁰⁹-GLIQKHRRRQ

Val⁴⁰⁹ Gly

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Asp⁴⁰⁰	Gly⁴⁰¹	Glu⁴⁰²	Arg⁴⁰³	Gly⁴⁰⁴	Cys⁴⁰⁵	Thr⁴⁰⁶	Phe⁴⁰⁷	Leu⁴⁰⁸	Val⁴⁰⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gly⁴¹⁰	Leu⁴¹¹	Ile⁴¹²	Gln⁴¹³	Lys⁴¹⁴	His⁴¹⁵	Arg⁴¹⁶	Arg⁴¹⁷	Arg⁴¹⁸	Gln⁴¹⁹

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 31] GERGCTFLVG⁴¹⁰-LIQKHRRRQR

Gly⁴¹⁰ Leu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Gly⁴⁰¹	Glu⁴⁰²	Arg⁴⁰³	Gly⁴⁰⁴	Cys⁴⁰⁵	Thr⁴⁰⁶	Phe⁴⁰⁷	Leu⁴⁰⁸	Val⁴⁰⁹	Gly⁴¹⁰

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Leu⁴¹¹	Ile⁴¹²	Gln⁴¹³	Lys⁴¹⁴	His⁴¹⁵	Arg⁴¹⁶	Arg⁴¹⁷	Arg⁴¹⁸	Gln⁴¹⁹	Arg⁴²⁰

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 32] KHRRRQRKMG⁴²³-EDMHTIGFGI

Gly⁴²³ Glu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Lys⁴¹⁴	His⁴¹⁵	Arg⁴¹⁶	Arg⁴¹⁷	Arg⁴¹⁸	Gln⁴¹⁹	Arg⁴²⁰	Lys⁴²¹	Met⁴²²	Gly⁴²³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Glu⁴²⁴	Asp⁴²⁵	Met⁴²⁶	His⁴²⁷	Thr⁴²⁸	Ile⁴²⁹	Gly⁴³⁰	Phe⁴³¹	Gly⁴³²	Ile⁴³³

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 33] LTGQTNIHLS⁴⁴⁹-KNFFLTTRAR

Ser⁴⁴⁹ Lys

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Leu⁴⁴⁰	Thr⁴⁴¹	Gly⁴⁴²	Gln⁴⁴³	Thr⁴⁴⁴	Asn⁴⁴⁵	Ile⁴⁴⁶	His⁴⁴⁷	Leu⁴⁴⁸	Ser⁴⁴⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Lys⁴⁵⁰	Asn⁴⁵¹	Phe⁴⁵²	Phe⁴⁵³	Leu⁴⁵⁴	Thr⁴⁵⁵	Thr⁴⁵⁶	Arg⁴⁵⁷	Ala⁴⁵⁸	Arg⁴⁵⁹

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 34] FFLTTRARER⁴⁶¹-SDTFINLREV

Arg⁴⁶¹ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Phe⁴⁵²	Phe⁴⁵³	Leu⁴⁵⁴	Thr⁴⁵⁵	Thr⁴⁵⁶	Arg⁴⁵⁷	Ala⁴⁵⁸	Arg⁴⁵⁹	Glu⁴⁶⁰	Arg⁴⁶¹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser⁴⁶²	Asp⁴⁶³	Thr⁴⁶⁴	Phe⁴⁶⁵	Ile⁴⁶⁶	Asn⁴⁶⁷	Leu⁴⁶⁸	Arg⁴⁶⁹	Glu⁴⁷⁰	Val⁴⁷¹

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 35] FLTTRARERS⁴⁶²-DTFINLREVL

Ser⁴⁶² Asp

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Phe⁴⁵³	Leu⁴⁵⁴	Thr⁴⁵⁵	Thr⁴⁵⁶	Arg⁴⁵⁷	Ala⁴⁵⁸	Arg⁴⁵⁹	Glu⁴⁶⁰	Arg⁴⁶¹	Ser⁴⁶²

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Asp⁴⁶³	Thr⁴⁶⁴	Phe⁴⁶⁵	Ile⁴⁶⁶	Asn⁴⁶⁷	Leu⁴⁶⁸	Arg⁴⁶⁹	Glu⁴⁷⁰	Val⁴⁷¹	Leu⁴⁷²

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 36] RERSDTFINL⁴⁶⁸-REVLNRFKLP

Leu⁴⁶⁸ Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Arg⁴⁵⁹	Glu⁴⁶⁰	Arg⁴⁶¹	Ser⁴⁶²	Asp⁴⁶³	Thr⁴⁶⁴	Phe⁴⁶⁵	Ile⁴⁶⁶	Asn⁴⁶⁷	Leu⁴⁶⁸

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg⁴⁶⁹	Glu⁴⁷⁰	Val⁴⁷¹	Leu⁴⁷²	Asn⁴⁷³	Arg⁴⁷⁴	Phe⁴⁷⁵	Lys⁴⁷⁶	Leu⁴⁷⁷	Pro⁴⁷⁸

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 37] ERSDTFINLR⁴⁶⁹-EVLNRFKLPP

Arg⁴⁶⁹ Glu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Glu⁴⁶⁰	Arg⁴⁶¹	Ser⁴⁶²	Asp⁴⁶³	Thr⁴⁶⁴	Phe⁴⁶⁵	Ile⁴⁶⁶	Asn⁴⁶⁷	Leu⁴⁶⁸	Arg⁴⁶⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Glu⁴⁷⁰	Val⁴⁷¹	Leu⁴⁷²	Asn⁴⁷³	Arg⁴⁷⁴	Phe⁴⁷⁵	Lys⁴⁷⁶	Leu⁴⁷⁷	Pro⁴⁷⁸	Pro⁴⁷⁹

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 38] KNGDFCIRVF⁵⁰²-SEKKADYQTV

Phe⁵⁰² Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Lys⁴⁹³	Asn⁴⁹⁴	Gly⁴⁹⁵	Asp⁴⁹⁶	Phe⁴⁹⁷	Cys⁴⁹⁸	Ile⁴⁹⁹	Arg⁵⁰⁰	Val⁵⁰¹	Phe⁵⁰²

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser⁵⁰³	Glu⁵⁰⁴	Lys⁵⁰⁵	Lys⁵⁰⁶	Ala⁵⁰⁷	Asp⁵⁰⁸	Tyr⁵⁰⁹	Gln⁵¹⁰	Thr⁵¹¹	Val⁵¹²

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 39] RVFSEKKADY⁵⁰⁹-QTVDDEIEAN

Tyr⁵⁰⁹ Gln

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Arg⁵⁰⁰	Val⁵⁰¹	Phe⁵⁰²	Ser⁵⁰³	Glu⁵⁰⁴	Lys⁵⁰⁵	Lys⁵⁰⁶	Ala⁵⁰⁷	Asp⁵⁰⁸	Tyr⁵⁰⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gln⁵¹⁰	Thr⁵¹¹	Val⁵¹²	Asp⁵¹³	Asp⁵¹⁴	Glu⁵¹⁵	Ile⁵¹⁶	Glu⁵¹⁷	Ala⁵¹⁸	Asn⁵¹⁹

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 40] EKKADYQTVD⁵¹³-DEIEANIEEI

Asp⁵¹³ Asp

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Glu⁵⁰⁴	Lys⁵⁰⁵	Lys⁵⁰⁶	Ala⁵⁰⁷	Asp⁵⁰⁸	Tyr⁵⁰⁹	Gln⁵¹⁰	Thr⁵¹¹	Val⁵¹²	Asp⁵¹³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Asp⁵¹⁴	Glu⁵¹⁵	Ile⁵¹⁶	Glu⁵¹⁷	Ala⁵¹⁸	Asn⁵¹⁹	Ile⁵²⁰	Glu⁵²¹	Glu⁵²²	Ile⁵²³

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 41] DYQTVDDEIE⁵¹⁷-ANIEEIEANE

Glu⁵¹⁷ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Asp⁵⁰⁸	Tyr⁵⁰⁹	Gln⁵¹⁰	Thr⁵¹¹	Val⁵¹²	Asp⁵¹³	Asp⁵¹⁴	Glu⁵¹⁵	Ile⁵¹⁶	Glu⁵¹⁷

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala⁵¹⁸	Asn⁵¹⁹	Ile⁵²⁰	Glu⁵²¹	Glu⁵²²	Ile⁵²³	Glu⁵²⁴	Ala⁵²⁵	Asn⁵²⁶	Glu⁵²⁷

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 42] EIEANIEEIE⁵²⁴-ANEEDIGDGF

Glu⁵²⁴ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Glu⁵¹⁵	Ile⁵¹⁶	Glu⁵¹⁷	Ala⁵¹⁸	Asn⁵¹⁹	Ile⁵²⁰	Glu⁵²¹	Glu⁵²²	Ile⁵²³	Glu⁵²⁴

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala⁵²⁵	Asn⁵²⁶	Glu⁵²⁷	Glu⁵²⁸	Asp⁵²⁹	Ile⁵³⁰	Gly⁵³¹	Asp⁵³²	Gly⁵³³	Phe⁵³⁴

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 43] IEANIEEIEA⁵²⁵-NEEDIGDGFR

Ala⁵²⁵ Asn

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ile⁵¹⁶	Glu⁵¹⁷	Ala⁵¹⁸	Asn⁵¹⁹	Ile⁵²⁰	Glu⁵²¹	Glu⁵²²	Ile⁵²³	Glu⁵²⁴	Ala⁵²⁵

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Asn⁵²⁶	Glu⁵²⁷	Glu⁵²⁸	Asp⁵²⁹	Ile⁵³⁰	Gly⁵³¹	Asp⁵³²	Gly⁵³³	Phe⁵³⁴	Arg⁵³⁵

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 44] TKIQKYQKIY⁶¹¹-REIDVDRSGT

Tyr⁶¹¹ Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Thr⁶⁰²	Lys⁶⁰³	Ile⁶⁰⁴	Gln⁶⁰⁵	Lys⁶⁰⁶	Tyr⁶⁰⁷	Gln⁶⁰⁸	Lys⁶⁰⁹	Ile⁶¹⁰	Tyr⁶¹¹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg⁶¹²	Glu⁶¹³	Ile⁶¹⁴	Asp⁶¹⁵	Val⁶¹⁶	Asp⁶¹⁷	Arg⁶¹⁸	Ser⁶¹⁹	Gly⁶²⁰	Thr⁶²¹

Sequence conservation (by blast) Sequence conservation (by blast)

[Site 45] KIYREIDVDR⁶¹⁸-SGTMNSYEMR

Arg⁶¹⁸ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Lys⁶⁰⁹	Ile⁶¹⁰	Tyr⁶¹¹	Arg⁶¹²	Glu⁶¹³	Ile⁶¹⁴	Asp⁶¹⁵	Val⁶¹⁶	Asp⁶¹⁷	Arg⁶¹⁸

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser⁶¹⁹	Gly⁶²⁰	Thr⁶²¹	Met⁶²²	Asn⁶²³	Ser⁶²⁴	Tyr⁶²⁵	Glu⁶²⁶	Met⁶²⁷	Arg⁶²⁸

Sequence conservation (by blast) Sequence conservation (by blast)

* References

[PubMed ID: 23495712] Weber H, Muller L, Jonas L, Schult C, Sparmann G, Schuff-Werner P, Calpain mediates caspase-dependent apoptosis initiated by hydrogen peroxide in pancreatic acinar AR42J cells. Free Radic Res. 2013 May;47(5):432-46. doi: 10.3109/10715762.2013.785633. Epub

» More (9) « Hide Below

[PubMed ID: 22711986] ... Wang CF, Huang YS, Calpain 2 activated through N-methyl-D-aspartic acid receptor signaling cleaves CPEB3 and abrogates CPEB3-repressed translation in neurons. Mol Cell Biol. 2012 Aug;32(16):3321-32. doi: 10.1128/MCB.00296-12. Epub 2012 Jun

[PubMed ID: 22547201] ... Xu PT, Li Q, Sheng JJ, Chang H, Song Z, Yu ZB, Passive stretch reduces calpain activity through nitric oxide pathway in unloaded soleus muscles. Mol Cell Biochem. 2012 Aug;367(1-2):113-24. doi: 10.1007/s11010-012-1325-8. Epub

[PubMed ID: 22870316] ... Miao Y, Dong LD, Chen J, Hu XC, Yang XL, Wang Z, Involvement of calpain/p35-p25/Cdk5/NMDAR signaling pathway in glutamate-induced neurotoxicity in cultured rat retinal neurons. PLoS One. 2012;7(8):e42318. doi: 10.1371/journal.pone.0042318. Epub 2012 Aug 1.

[PubMed ID: 21549862] ... Chou JS, Impens F, Gevaert K, Davies PL, m-Calpain activation in vitro does not require autolysis or subunit dissociation. Biochim Biophys Acta. 2011 Jul;1814(7):864-72. doi: 10.1016/j.bbapap.2011.04.007.

[PubMed ID: 11102442] ... Hosfield CM, Moldoveanu T, Davies PL, Elce JS, Jia Z, Calpain mutants with increased Ca2+ sensitivity and implications for the role of the C(2)-like domain. J Biol Chem. 2001 Mar 9;276(10):7404-7. Epub 2000 Dec 1.

[PubMed ID: 10601010] ... Hosfield CM, Elce JS, Davies PL, Jia Z, Crystal structure of calpain reveals the structural basis for Ca(2+)-dependent protease activity and a novel mode of enzyme activation. EMBO J. 1999 Dec 15;18(24):6880-9.

[PubMed ID: 7635186] ... Arthur JS, Gauthier S, Elce JS, Active site residues in m-calpain: identification by site-directed mutagenesis. FEBS Lett. 1995 Jul 24;368(3):397-400.

[PubMed ID: 7982961] ... Graham-Siegenthaler K, Gauthier S, Davies PL, Elce JS, Active recombinant rat calpain II. Bacterially produced large and small subunits associate both in vivo and in vitro. J Biol Chem. 1994 Dec 2;269(48):30457-60.

[PubMed ID: 8218419] ... DeLuca CI, Davies PL, Samis JA, Elce JS, Molecular cloning and bacterial expression of cDNA for rat calpain II 80 kDa subunit. Biochim Biophys Acta. 1993 Oct 19;1216(1):81-93.

organism	matching
N/A	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90
Rattus norvegicus	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90
Mus musculus	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 31 DYETLRNECLEAGALFQDPSFPALPSSLGY#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90
Homo sapiens	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|+\|\|+\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 31 DYEALRNECLEAGTLFQDPSFPAIPSALGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90
Macaca mulatta	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|+\|\|+\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 31 DYEALRNECLEAGTLFQDPSFPAIPSALGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90
Homo sapiens	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|+\|\|+\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 31 DYEALRNECLEAGTLFQDPSFPAIPSALGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90
Gallus gallus	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|\|\| \|+ \|\|+\|+\| \|\|+\|\| \|\|\| \|++\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|+\|+ \|\|\|\|\|+\| Sbjct 34 DYEALKQECIESGTLFRDPQFPAGPTALGF#KELGPYSSKTRGVEWKRPSELVDDPQFIVG 93
Xenopus laevis	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|\|\|+\|+ +\|\|\|+\| \|\|+\|\| \|\|\| \|\|\|\|\|\|#\|\|\|\|\| \|\|\|\|\|\|++\|\|\|\|+\|\| \|\|\|\|\|+\| Sbjct 34 DYESLKQQCLESGILFEDPHFPATPSSLGF#KELGPGSSKTRGVQWKRPSEIVDDPQFIVG 93
Coturnix coturnix	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|\|\| \|+ \|+\|+\|\|\|\|+\|\| \|\|\| \|++\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|+\|\|\|\|\|+\|+ \|\|\|\|\|+\| Sbjct 34 DYEALKQACIESGALFRDPQFPAGPTALGF#KELGPHSSKTRGVEWKRPSELVDDPQFIVG 93
Xenopus laevis	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|\|\|+\|+ +\|+\|+\| \|\|+\|\|+\|\|\|+\|\| \|\|\|#\|\|\|\|\| \|\|\|\|\|\|++\|\|\|\|++\| \|\|\|\|\|\|\| Sbjct 34 DYESLKQQCVESGILFEDPNFPAIPSILGF#KELGPGSSKTRGVQWKRPSDIVDDPQFIIG 93
Danio rerio	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|\|\| \|\| \|\|+\|+\| \|\|+\|\| \|\|\|+\| \|\|\|\|#\|\|\| \| \|\|\|\|\|\|++\| \|\|\|\|+ +\|\|\|\|+\| Sbjct 34 DYEELRRECVESGRLFEDPCFPAVPQSLGF#KELAPNSSKTRGVKWIRPTELSENPQFIVG 93
Tetraodon nigroviridis	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|\|\| \|+ +\|+\|+\| \|\|+\|\| \| \| \|+\|\|\|\|#\|\|\| \|+\|\|\|\|\|\|+\|\|+\|\|\|\|+ \|\|\|\|\|+\| Sbjct 29 DYEALKQQCVESGCLFEDPLFLAQPASLGF#KELAPHSSKTRGVEWQRPTELTGDPQFIVG 88
Xenopus tropicalis	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|\|\|+\|+ +\| \|+\|+\|\|+\|\|+\|\| + \|\|\|\|\|#\|\|\|\|\| +\|\|\|+\|++\|\|\|\| +\| \|\|\|\|\|+\| Sbjct 34 DYESLKQQCRESGSLFEDPNFPPIGSSLGF#KELGPGTSKTKGVQWKRPHDIADDPQFIVG 93
Oncorhynchus mykiss	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|+\| \|\| +\|\| \| \|\| \|\|+\|\|\| \| \|\|\|\|# \|\|\|\| \| \|\|\| ++\|\|\|\| \|+\|+ \|+\|\|+\| Sbjct 31 DFEALRAQCLNRGGLFSDPTFPAAPESLGF#NELGPGSYKTRAVQWKRPGELCSSPEFIVG 90
Bos taurus	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFII 89 \|\|\| \|\| \|\|+ \|\|\|\|+\| +\|\| +\| \|\|\|\|#\|\|\|\|\| \|\|\|\| \|\|+\|\|\|\|\|\|+ ++\|\|\|\|+ Sbjct 41 DYEQLRVHCLQRGALFRDEAFPPVPQSLGF#KELGPNSSKTYGIKWKRPTELFSNPQFIV 99
Canis familiaris	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|+\| \|\| +\|\|+\|\| \|\|+\|\| \|\|\| \|\|+\|\| #\|+\|\|\| \| +\|+\|\| \|\|\|\| \|+\|\|+\|\|\|\|+\| Sbjct 124 DFEKLRQQCLDAGVLFKDPEFPACPSALGH#KDLGPQSPQTQGIVWKRPPELCANPQFIVG 183
Sus scrofa	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFII 89 \|\|\| \|\| \|\|++\|+\|\|+\| +\|\| +\| \|\|\|\|#\|\|\|\|\| \|\|\|\| \|++\|\|\|\|\|\|+ ++\|\|\|\|+ Sbjct 41 DYEQLRAHCLQSGSLFRDEAFPPVPQSLGF#KELGPNSSKTYGVKWKRPTELFSNPQFIV 99
Gallus gallus	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFII 89 \|\| \|\|++\|\| +\|+\|\|+\| +\|\| \|\|\|\|\|#+\|\|\|\| \|\|\|\|\|\|+ \|\|\|\|\|\|+\| \|\|\|\|+ Sbjct 41 DYAALRDDCLRSGSLFRDETFPPSASSLGF#RELGPGSSKTRGVTWKRPTELCRHPQFIV 99
Rattus norvegicus	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFII 89 \|\|\| \|\| \|\|+ \| \|\|\|\| +\|\| + \|\|\|\|#\|\|\|\|\| \|\|\|\| \|\|+\|\|\|\|\|\|+ ++\|\|\|\|+ Sbjct 41 DYENLRARCLQNGVLFQDDAFPPVSHSLGF#KELGPNSSKTYGIKWKRPTELLSNPQFIV 99
Pongo pygmaeus	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFII 89 \|\|\| \|\| \|\|++\| \|\|+\| +\|\| +\| \|\|\|+#\|+\|\|\| \|\|\|\| \|\|+\|\|\|\|\|\|+ ++\|\|\|\|+ Sbjct 41 DYEQLRVRCLQSGTLFRDEAFPPVPQSLGY#KDLGPNSSKTYGIKWKRPTELLSNPQFIV 99
synthetic construct	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFII 89 \|\|\| \|\| \|\|++\| \|\|+\| +\|\| +\| \|\|\|+#\|+\|\|\| \|\|\|\| \|\|+\|\|\|\|\|\|+ ++\|\|\|\|+ Sbjct 41 DYEQLRVRCLQSGTLFRDEAFPPVPQSLGY#KDLGPNSSKTYGIKWKRPTELLSNPQFIV 99
Stizostedion vitreum vitreum	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFII 89 \|\|\| \|\| \|\|\|+ +\|\|+\| +\|\|\| \|\|\|\|\|#\|\|\|\| \| \| \|\|+ \|+\|\|\|\| +\|\|\|\|\|+ Sbjct 31 DYEALRRRCLESESLFKDDAFPAETSSLGF#KELGVGSYKIRGVSWERPTEFTSDPQFIV 89
Danio rerio	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFII 89 \|\|\|\|\|\| + ++ \|\|+\|\| \| \| \|\|\|\|\|# \|\|\|\| \|\|\|\|+\|+ \| \|\| \|+\|\| \|\|\|\|+ Sbjct 36 DYETLRAQSQQSRRLFEDPMFTASSSSLGF#NELGPRSSKTQGVRWMRPKEMCARPQFIV 94
Canis familiaris	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFII 89 ++\| \|+ \|\| \| \|\|+\| \|\|\| \|\|\|\|\|\|#\|+\|\|\| \| + \| \|+\|\| +\| + \| \|\|+ Sbjct 113 NFEDLQAACLRKGELFEDSFFPAEPSSLGF#KDLGPNSRNVQNICWQRPGQITSSPHFIV 171
Oryctolagus cuniculus	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 \|+ \|\| \|\| \|\|+\| \|\|\| \|\|\| # \|\| +\| + \| \|\|\|\| \|\|\| +\|+\|\|\|\| Sbjct 36 DFVVLRQRCLAQKCLFEDRVFPAGVQSLGA#HELSQ-KTKMKAITWKRPQEICENPRFIIG 94
Oncorhynchus mykiss	Query 32 YETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFIIG 90 +\| \|\| \|\|\|+ \|\|+\|\| \|\|\| \|\|\| +# + \| + \|\|\|\|\| \|+\| +\|+\|\|+\| Sbjct 15 FEQLRQECLQKKKLFEDPDFPACDSSLYY#SQSVPIN-----FEWKRPGELCENPEFIVG 68
Drosophila melanogaster	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFII 89 \|\|\|\|+ \| \|\| +\|+\|\|+\|\| \|\|\| \|\| \|# \| \|\|\| \|\| \|\| +\|\|\| + Sbjct 51 DYETILNSCLASGSLFEDPLFPASNESLQF#SR-----RPDRHIEWLRPHEIAENPQFFV 104
Drosophila melanogaster	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFII 89 \|\|\|\|+ \| \|\| +\|+\|\|+\|\| \|\|\| \|\| \|# \| \|\|\| \|\| \|\| +\|\|\| + Sbjct 74 DYETILNSCLASGSLFEDPLFPASNESLQF#SR-----RPDRHIEWLRPHEIAENPQFFV 127
Drosophila pseudoobscura	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFII 89 \|+ +\|\|+ \|+ \| +\|+\|\| \|\|\| +\|\| \|# + \| \|\|\| \|\| +\| \|\|\|\| + Sbjct 251 DFASLRDSCVINGTMFEDPDFPANNASLMF#SK-----RPDRYIEWLRPGDIVGDPQFFV 304
Caenorhabditis elegans	Query 31 DYETLRNECLEAGALFQDPSFPALPSSLGF#KELGPYSSKTRGIEWKRPTEICADPQFI 88 \|+\|\| \| +\|\| \|\|+\|\| \|\|\| +\|\| +#+ + \| \|\|\|\| \|\| \|+\|\| \| Sbjct 226 DFETEREKCLRNKTLFEDPEFPATAASLYY#R-----TPPRDRIIWKRPGEIIANPQLI 278

organism	matching
Rattus norvegicus	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146
Homo sapiens	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLNQSFQENYAGIFHFQFWQY 146
N/A	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLNQSFQENYAGIFHFQFWQY 146
Macaca mulatta	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLNQSFQENYAGIFHFQFWQY 146
Homo sapiens	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLNQSFQENYAGIFHFQFWQY 146
Mus musculus	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPPDQSFQENYAGIFHFQFWQY 146
Xenopus tropicalis	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|+\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 87 FIAGGATRTDICQGALGDCWLLAAIASLTL#NEQILSRVVPKDQSFQENYAGIFHFQFWQY 146
Tetraodon nigroviridis	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\| +\|\|\|\|\|\| \|\| \| \|+\|\|\|\|\|\|\|\|\|\|\|+ Sbjct 87 FIVGGATRTDICQGALGDCWLLAAIASLTL#NEFVLARVVPGDQGFGEDYAGIFHFQFWQF 146
Danio rerio	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|++\|\|\|\|\|\| \|\| \| ++\|\|\|\|\|\|\|\|\|\|\|+ Sbjct 87 FIVGGATRTDICQGALGDCWLLAAIASLTL#NEDVLARVVPSDQGFGQDYAGIFHFQFWQF 146
Gallus gallus	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 87 FIAGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPRDQSFQDEYAGIFHFQFWQY 146
Oncorhynchus mykiss	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|++++\|\|\|\|+ \|\|\| \|+\|\|\|\|\|\|\|\|\|\|\|+ Sbjct 87 FIVGGATRTDICQGALGDCWLLAAIASLTL#NEDVMSRVVPVGQSFGESYAGIFHFQFWQF 146
Xenopus laevis	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|#\|\|++\| \|\|\|\| \|\|\|\|\|+\|\|\|\|\|\|\|\| \|\|+ Sbjct 90 FIIGGATRTDICQGALGDCWLLAAIGSLTL#NEDLLHRVVPHGQSFQEDYAGIFHFQIWQF 149
Coturnix coturnix	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|#\|\|\|+\| \|\|\|\| \|\|\|\|\|+\|\|\|\|\|\|\|\| \|\|+ Sbjct 90 FIVGGATRTDICQGALGDCWLLAAIGSLTL#NEELLHRVVPHGQSFQEDYAGIFHFQIWQF 149
Canis familiaris	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\| \|\|\|#\|\| +\| \|\|+\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+\| Sbjct 111 FIIGGANRTDICQGDLGDCWFLAAIACLTL#NERLLFRVIPHDQSFTENYAGIFHFQFWRY 170
Xenopus laevis	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|#\|\|++\| \|\|\|\| \|\|\|\|\| \|\|\|\|\|\|\|\| \|\|+ Sbjct 90 FIVGGATRTDICQGALGDCWLLAAIGSLTL#NEDLLHRVVPHGQSFQEEYAGIFHFQIWQF 149
Ovis aries	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\| \|\|\|#\|\| +\| \|\|+\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+\| Sbjct 111 FIIGGANRTDICQGDLGDCWFLAAIACLTL#NERLLFRVIPHDQSFTENYAGIFHFQFWRY 170
Rattus norvegicus	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\| \|\| \|\|\|\| \|\|\|\|\| \|\|\|\|\|\|\|\| \|\|+ Sbjct 97 FIVDGATRTDICQGALGDCWLLAAIASLTL#NETILHRVVPYGQSFQEGYAGIFHFQLWQF 156
Sus scrofa	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\| \|\|\|#\|+ +\| \|\|+\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+\| Sbjct 110 FIIGGANRTDICQGDLGDCWFLAAIACLTL#NKRLLFRVIPHDQSFTENYAGIFHFQFWRY 169
Bos taurus	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|+\|\|\| \|\|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\| \|\|\|#\|+ +\| \|\|+\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+\| Sbjct 111 FIVGGANRTDICQGDLGDCWFLAAIACLTL#NKRLLFRVIPHDQSFTENYAGIFHFQFWRY 170
Oryctolagus cuniculus	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\| \|\|\|#\|\| +\| \|\|+\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+\| Sbjct 91 FIIGGANRTDICQGDLGDCWFLAAIACLTL#NERLLFRVIPHDQSFTENYAGIFHFQFWRY 150
Oncorhynchus mykiss	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|+\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#++\| \|\|\|\|\|\| \|\| \| +\|\|\|\|\|\|\|\|\|\|\|+ Sbjct 65 FIVGGADRTDICQGALGDCWLLAAIASLTL#HKETLARVVPNDQGFDRSYAGIFHFQFWQH 124
Gallus gallus	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\| \|\| \|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\| \|\|+ Sbjct 97 FIVDGATRTDICQGALGDCWLLAAIASLTL#NETILHRVVPHGQSFQNGYAGIFHFQIWQF 156
Macaca fascicularis	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|\| \|\| \|\|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\| \|\|\|#\|+ +\| \|\|+\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+\| Sbjct 111 FIIDGANRTDICQGDLGDCWFLAAIACLTL#NQRLLFRVIPHDQSFIENYAGIFHFQFWRY 170
Stizostedion vitreum vitreum	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQE-NYAGIFHFQFWQ 145 \|\|+ \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|+\|+\|\|\|\|\|\| \|\|\| + \|\|\|\|\|\|\|\|\|\|\| Sbjct 87 FIVSGASRTDICQGALGDCWLLAAIASLTL#NQEVLARVVPHGQSFSKGEYAGIFHFQFWQ 146 Query 146 Y 146 + Sbjct 147 F 147
Pongo pygmaeus	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|+ +\| \|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\| \|\|+ Sbjct 97 FIVDGATRTDICQGALGDCWLLAAIASLTL#NDTLLHRVVPHGQSFQNGYAGIFHFQLWQF 156
synthetic construct	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|+ +\| \|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\| \|\|+ Sbjct 97 FIVDGATRTDICQGALGDCWLLAAIASLTL#NDTLLHRVVPHGQSFQNGYAGIFHFQLWQF 156
Danio rerio	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|+ +\| \|\|\|\| \| \| \|\|\|\|\|\|\|\|\|\|\|+ Sbjct 92 FIVDGATRTDICQGALGDCWLLAAIASLTL#NDNLLHRVVPHGQDFDSRYAGIFHFQFWQF 151
Canis familiaris	Query 87 FIIGGATRTDICQGALGDCWLLAAIASLTL#NEEILARVVPLDQSFQENYAGIFHFQFWQY 146 \|\|+ \| + \|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\| \|\|\| # ++\| \|\|\|\| \|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|+ Sbjct 169 FIVNGFSPTDICQGVLGDCWLLAAIGSLTT#CPKLLHRVVPKGQSFRKNYAGIFHFQFWQF 228

organism	matching
Mus musculus	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231
N/A	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231
Rattus norvegicus	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231
Homo sapiens	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELKKPPPNLFKIIQKA 231
Homo sapiens	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELKKPPPNLFKIIQKA 231
Macaca mulatta	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELKKPPPNLFKIIQKA 231
Xenopus tropicalis	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\| \|\|#\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\| Sbjct 172 EGSEFWSALMEKAYAKLNGSYEALSGGTTT#EGFEDFTGGLAEWYELRKAPPNLFKIIQKA 231
Oncorhynchus mykiss	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|#\|\|\|\|\|\|\|\|\| \|\| \|+\|\|\|\|\|\|\|+\|+\|\|+\|\| Sbjct 172 EGSEFWSALLEKAYAKVNGCYEALSGGSTT#EGFEDFTGGNAENYDLRKPPPNMFQIIKKA 231
Gallus gallus	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\| \|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|\|\|\| Sbjct 172 EGSEFWSALLEKAYAKLNGSYEALSGGTTT#EGFEDFTGGIAEWYELQKAPPNLFKIIQKA 231
Tetraodon nigroviridis	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|+\|\|\|\|\| \|\|\|+\|++\|\| Sbjct 172 EGREFWSALLEKAYAKVNGCYEALSGGSTT#EGFEDFTGGIAENYDLRKPPSNLFQILKKA 231
Gallus gallus	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|+#\|\|\|\|\|\|\|\|\|+ \|\|\|+\|\|\|\|\| +\|++\|\| \|\| Sbjct 182 EGTEFWSALLEKAYAKVNGCYEALSGGSTS#EGFEDFTGGVTEWYDLRKPPADLYQIILKA 241
Danio rerio	Query 173 GSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+ \| \|\|\|+\|\|+\|\| Sbjct 173 GSEFWSALLEKAYAKVNGCYEALSGGSTT#EGFEDFTGGIAEMYELKSAPTNLFQIIKKA 231
Stizostedion vitreum vitreum	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|++\| \|\| \|\|\|\|\| Sbjct 173 EGREFWSALLEKAYAKLNGCYEALSGGSTT#EGFEDFTGGIAEVYELKQPDARLFHIIQKA 232
synthetic construct	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+#\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\| \| +\|++\|\| \|\| Sbjct 182 EGNEFWSALLEKAYAKVNGSYEALSGGSTS#EGFEDFTGGVTEWYELRKAPSDLYQIILKA 241
Pongo pygmaeus	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+#\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\| \| +\|++\|\| \|\| Sbjct 182 EGNEFWSALLEKAYAKVNGSYEALSGGSTS#EGFEDFTGGVTEWYELRKAPSDLYQIILKA 241
Canis familiaris	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 +\|+\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+#\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\| \| +\|++\|\| \|\| Sbjct 182 QGNEFWSALLEKAYAKVNGSYEALSGGSTS#EGFEDFTGGVTEWYELRKAPSDLYQIILKA 241
Xenopus laevis	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|+\|\|+\| #\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\| \|\|\|\|\|+\|\|\|\|\| Sbjct 172 EGDEFWSALLEKAYAKLNGSYEALTGGSTI#EGFEDFTGGIAEVYELKKAPPNLFQIIQKA 231
Bos taurus	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 +\|+\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+#\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\| \| +\|+ \|\| \|\| Sbjct 182 QGNEFWSALLEKAYAKVNGSYEALSGGSTS#EGFEDFTGGVTEWYELRKAPSDLYNIILKA 241
Sus scrofa	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 +\|+\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+#\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\| \| +\|+ \|\| \|\| Sbjct 182 QGNEFWSALLEKAYAKVNGSYEALSGGSTS#EGFEDFTGGVTEWYELRKAPSDLYSIILKA 241
Danio rerio	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| #\|\|\|\|\|\|\|\|\|+ \| \|\|\|+\| \| +\|\| \|\| +\| Sbjct 177 EGGEFWSALLEKAYAKLNGCYEALSGGSTC#EGFEDFTGGVTEMYELKKAPADLFSIIGRA 236
Rattus norvegicus	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 +\|+\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\| \|+#\| \|\|\|\|\|\|\|+ \|\|\|+\|+\| \| +\|++\|\| \|\| Sbjct 182 QGNEFWSALLEKAYAKVNGSYEALSGGCTS#EAFEDFTGGVTEWYDLQKAPSDLYQIILKA 241
Xenopus laevis	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \| +\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \|\|+\|\|\|\|+\|\|#\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\| \| +\| \|\|\|++\| Sbjct 175 ECTEFWSALLEKAYAKLNGSYESLSGGSTT#EGFEDFTGGVAEMYELRKAPRDLDKIIKRA 234
Coturnix coturnix	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \| +\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|+\|\|\|\|+\|\|#\|\|\|\|\|\|\|\|\|+\|\| \|+\|++ \| \|+ \|\|+\|\| Sbjct 175 ECTEFWSALLEKAYAKLNGCYESLSGGSTT#EGFEDFTGGVAEMYDLKRAPRNMGHIIRKA 234
Drosophila melanogaster	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \| +\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \|\|\|\| \|\|+\| #\| \|\|\|\|\|\|++\|\|\|+\|++ \| \|\|\| \|+\|\|\| Sbjct 210 EKNEFWSALLEKAYAKLHGSYEALKGGSTC#EAMEDFTGGVSEWYDLKEAPGNLFTILQKA 269
Drosophila pseudoobscura	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \| +\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \|\|\|\| \|\| \| #\| \|\|\|\|\|\|+ \|\|\|++++ \|\|\|\|\| \|+ \|\| Sbjct 387 ERNEFWSALLEKAYAKLHGSYEALKGGTTC#EAMEDFTGGVTEWYDIKEAPPNLFSIMMKA 446
Drosophila melanogaster	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \| +\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \|\|\|\| \|\|+\| #\| \|\|\|\|\|\|++\|\|\|+\|++ \| \|\|\| \|+\|\|\| Sbjct 187 EKNEFWSALLEKAYAKLHGSYEALKGGSTC#EAMEDFTGGVSEWYDLKEAPGNLFTILQKA 246
Canis familiaris	Query 172 EGSEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 \| +\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\| #\|\|\|\|\|\|\|\|\|+ +\|+\|\|\| \|\| ++++\|\| Sbjct 254 ERTEFWSALLEKAYAKLNGSYEALSGGSTV#EGFEDFTGGVTYTIQLQKPPRNLLRMLRKA 313
Macaca fascicularis	Query 174 SEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 +\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \|\|\|\| \|\| \|\|#\| \|\|\|\|\|\|+ \|++\|+\| \| ++ \|\|++\|\| Sbjct 198 NEFWSALLEKAYAKLHGSYEALKGGNTT#EAMEDFTGGVTEFFEIRDAPSDMHKIMKKA 255
Oryctolagus cuniculus	Query 174 SEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 +\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \|\|\|\| \|\| \|\|#\| \|\|\|\|\|\|+ \|++\|++ \| +++\|\|++\|\| Sbjct 178 NEFWSALLEKAYAKLHGSYEALKGGNTT#EAMEDFTGGVTEFFEIKDAPRDMYKIMRKA 235
Oncorhynchus mykiss	Query 174 SEFWSALLEKAYAKINGCYEALSGGATT#EGFEDFTGGIAEWYELRKPPPNLFKIIQKA 231 +\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \|\|+\| \|\|+\| #\| \|\|\|\|\|\|+ \| \|\| + \| +\|\| \|++\|\| Sbjct 152 NEFWSALLEKAYAKLNGSYESLKGGSTL#EAMEDFTGGVGETYETKSAPTDLFNIMKKA 209

organism	matching
Mus musculus	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237
N/A	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237
Rattus norvegicus	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237
Homo sapiens	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELKKPPPNLFKIIQKALQKGSL 237
Macaca mulatta	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELKKPPPNLFKIIQKALQKGSL 237
Homo sapiens	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELKKPPPNLFKIIQKALQKGSL 237
Gallus gallus	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 178 SALLEKAYAKLNGSYEALSGGTTTEGFEDF#TGGIAEWYELQKAPPNLFKIIQKALQKGSL 237
Gallus gallus	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\|#\|\|\|+ \|\|\|+\|\|\|\|\| +\|++\|\| \|\|\|\|+\|\|\| Sbjct 188 SALLEKAYAKVNGCYEALSGGSTSEGFEDF#TGGVTEWYDLRKPPADLYQIILKALERGSL 247
Xenopus tropicalis	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|+\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|#\|\|\|+\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\|\| Sbjct 178 SALMEKAYAKLNGSYEALSGGTTTEGFEDF#TGGLAEWYELRKAPPNLFKIIQKALRTGSL 237
Oncorhynchus mykiss	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|#\|\|\| \|\| \|+\|\|\|\|\|\|\|+\|+\|\|+\|\|+\| \|+\| Sbjct 178 SALLEKAYAKVNGCYEALSGGSTTEGFEDF#TGGNAENYDLRKPPPNMFQIIKKAIESGAL 237
Tetraodon nigroviridis	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\| \|+\|\|\|\|\| \|\|\|+\|++\|\|\|\| \|+\| Sbjct 178 SALLEKAYAKVNGCYEALSGGSTTEGFEDF#TGGIAENYDLRKPPSNLFQILKKALEAGAL 237
Xenopus laevis	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\|#\|\|\|+ \|\|\|\|++\| \| +\|\| \|\| \|\|\|\|+\|\|\| Sbjct 188 SALLEKAYAKANGCYEALSGGSTSEGFEDF#TGGVTEWYEMKKAPKDLFNIILKALERGSL 247
synthetic construct	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+\|\|\|\|\|\|#\|\|\|+ \|\|\|\|\|\|\| \| +\|++\|\| \|\|\|\|+\|\|\| Sbjct 188 SALLEKAYAKVNGSYEALSGGSTSEGFEDF#TGGVTEWYELRKAPSDLYQIILKALERGSL 247
Canis familiaris	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+\|\|\|\|\|\|#\|\|\|+ \|\|\|\|\|\|\| \| +\|++\|\| \|\|\|\|+\|\|\| Sbjct 188 SALLEKAYAKVNGSYEALSGGSTSEGFEDF#TGGVTEWYELRKAPSDLYQIILKALERGSL 247
Danio rerio	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\| \|\|\|+ \| \|\|\|+\|\|+\|\|\|+ \|+\| Sbjct 178 SALLEKAYAKVNGCYEALSGGSTTEGFEDF#TGGIAEMYELKSAPTNLFQIIKKALDSGAL 237
Pongo pygmaeus	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+\|\|\|\|\|\|#\|\|\|+ \|\|\|\|\|\|\| \| +\|++\|\| \|\|\|\|+\|\|\| Sbjct 188 SALLEKAYAKVNGSYEALSGGSTSEGFEDF#TGGVTEWYELRKAPSDLYQIILKALERGSL 247
Bos taurus	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+\|\|\|\|\|\|#\|\|\|+ \|\|\|\|\|\|\| \| +\|+ \|\| \|\|\|\|+\|\|\| Sbjct 188 SALLEKAYAKVNGSYEALSGGSTSEGFEDF#TGGVTEWYELRKAPSDLYNIILKALERGSL 247
Sus scrofa	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+\|\|\|\|\|\|#\|\|\|+ \|\|\|\|\|\|\| \| +\|+ \|\| \|\|\|\|+\|\|\| Sbjct 188 SALLEKAYAKVNGSYEALSGGSTSEGFEDF#TGGVTEWYELRKAPSDLYSIILKALERGSL 247
Stizostedion vitreum vitreum	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\| \|\|\|++\| \|\| \|\|\|\|\| +\|\|\| Sbjct 179 SALLEKAYAKLNGCYEALSGGSTTEGFEDF#TGGIAEVYELKQPDARLFHIIQKAQSRGSL 238
Coturnix coturnix	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|+\|\|\|\|+\|\|\|\|\|\|\|\|#\|\|\|+\|\| \|+\|++ \| \|+ \|\|+\|\|\|\|+\|\|\| Sbjct 181 SALLEKAYAKLNGCYESLSGGSTTEGFEDF#TGGVAEMYDLKRAPRNMGHIIRKALERGSL 240
Danio rerio	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|#\|\|\|+ \| \|\|\|+\| \| +\|\| \|\| +\|+\|+\|\|\| Sbjct 183 SALLEKAYAKLNGCYEALSGGSTCEGFEDF#TGGVTEMYELKKAPADLFSIIGRAIERGSL 242
Rattus norvegicus	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\| \|+\| \|\|\|\|#\|\|\|+ \|\|\|+\|+\| \| +\|++\|\| \|\|\|\|+\|\|\| Sbjct 188 SALLEKAYAKVNGSYEALSGGCTSEAFEDF#TGGVTEWYDLQKAPSDLYQIILKALERGSL 247
Xenopus laevis	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\| \|\|+\|\|\|\|+\|\|\|\|\|\|\|\|#\|\|\|+\|\| \|\|\|\|\| \| +\| \|\|\|++\|\|\|+\|\|\| Sbjct 181 SALLEKAYAKLNGSYESLSGGSTTEGFEDF#TGGVAEMYELRKAPRDLDKIIKRALERGSL 240
Drosophila pseudoobscura	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|++\| \|\|\|\| \|\| \| \| \|\|\|#\|\|\|+ \|\|\|++++ \|\|\|\|\| \|+ \|\| \|+\|\|+ Sbjct 393 SALLEKAYAKLHGSYEALKGGTTCEAMEDF#TGGVTEWYDIKEAPPNLFSIMMKAAERGSM 452
Drosophila melanogaster	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|++\| \|\|\|\| \|\| \| \| \|\|\|#\|\|\|+ \|\|\|++++ \|\|\|\|\| \|+ \|\| \|+\|\|+ Sbjct 387 SALLEKAYAKLHGSYEALKGGTTCEAMEDF#TGGVTEWYDIKEAPPNLFSIMMKAAERGSM 446
Drosophila melanogaster	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|++\| \|\|\|\| \|\|+\| \| \|\|\|#\|\|\|++\|\|\|+\|++ \| \|\|\| \|+\|\|\| \|+ \|+ Sbjct 193 SALLEKAYAKLHGSYEALKGGSTCEAMEDF#TGGVSEWYDLKEAPGNLFTILQKAAERNSM 252
Canis familiaris	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\| \|\|\|\|\|\|#\|\|\|+ +\|+\|\|\| \|\| ++++\|\|+\|+ \|\| Sbjct 260 SALLEKAYAKLNGSYEALSGGSTVEGFEDF#TGGVTYTIQLQKPPRNLLRMLRKAVERSSL 319
Macaca fascicularis	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|++\| \|\|\|\| \|\| \|\|\| \|\|\|#\|\|\|+ \|++\|+\| \| ++ \|\|++\|\|+\|+\|\|\| Sbjct 202 SALLEKAYAKLHGSYEALKGGNTTEAMEDF#TGGVTEFFEIRDAPSDMHKIMKKAIERGSL 261
Oryctolagus cuniculus	Query 178 SALLEKAYAKINGCYEALSGGATTEGFEDF#TGGIAEWYELRKPPPNLFKIIQKALEKGSL 237 \|\|\|\|\|\|\|\|\|\|++\| \|\|\|\| \|\| \|\|\| \|\|\|#\|\|\|+ \|++\|++ \| +++\|\|++\|\|+\|+\| \| Sbjct 182 SALLEKAYAKLHGSYEALKGGNTTEAMEDF#TGGVTEFFEIKDAPRDMYKIMRKAIERGFL 241

#	organism	max score	hits	top seq
1	N/A	129.00	13	A Chain A, Crystal Structure Of M-Calpain
2	Rattus norvegicus	128.00	11	calpain 2
3	Mus musculus	127.00	21	m-calpain
4	Homo sapiens	123.00	7	AF261089_1 calpain large polypeptide L2
5	Macaca mulatta	123.00	2	PREDICTED: calpain 2, large subunit
6	Homo sapiens	123.00	2	calpain 2, large subunit
7	Gallus gallus	107.00	3	calpain 1, (mu/I) large subunit
8	Xenopus laevis	105.00	5	hypothetical protein LOC398772
9	Coturnix coturnix	104.00	1	quail calpain
10	Xenopus laevis	102.00	1	mu/m-calpain large subunit
11	Danio rerio	99.40	5	PREDICTED: similar to Calpain 1, (mu/I) large sub
12	Tetraodon nigroviridis	99.00	13	unnamed protein product
13	Xenopus tropicalis	95.50	5	CAPN2 protein
14	Oncorhynchus mykiss	93.20	1	m-calpain
15	Bos taurus	92.80	5	micromolar calcium activated neutral protease 1
16	Canis familiaris	91.30	7	PREDICTED: similar to calpain 8
17	Sus scrofa	89.40	4	calpain 1, large subunit
18	Gallus gallus	89.00	1	mu-calpain large subunit
19	Rattus norvegicus	88.60	1	calpain 1, large subunit
20	Pongo pygmaeus	87.00	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
21	synthetic construct	87.00	1	Homo sapiens calpain 1, (mu/I) large subunit
22	Stizostedion vitreum vitreum	84.30	1	putative calpain-like protein
23	Danio rerio	82.00	1	calpain 1, large subunit
24	Canis familiaris	67.80	1	PREDICTED: similar to Calpain-11 (Calcium-activat
25	Oryctolagus cuniculus	62.40	1	calpain 3, (p94)
26	Oncorhynchus mykiss	61.20	1	gill-specific calpain
27	Drosophila melanogaster	57.80	5	CAA55297.1
28	Drosophila melanogaster	57.80	3	Calpain-A CG7563-PB, isoform B
29	Drosophila pseudoobscura	52.80	1	GA20829-PA
30	Caenorhabditis elegans	52.40	2	CaLPain family member (clp-4)

organism	matching
Rattus norvegicus	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112
Mus musculus	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 53 ALPSSLGYKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112
Homo sapiens	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \|+\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 53 AIPSALGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112
Homo sapiens	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \|+\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 53 AIPSALGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112
Macaca mulatta	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \|+\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 53 AIPSALGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112
N/A	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDSWLLAAIA 112
Tetraodon nigroviridis	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \| \|+\|\|\|\|\|\|\| \|+\|\|\|\|\|\|+\|\|+\|\|\|\|+ # \|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 51 AQPASLGFKELAPHSSKTRGVEWQRPTELT#GDPQFIVGGATRTDICQGALGDCWLLAAIA 110
Gallus gallus	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \| \|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|+\|+ # \|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 56 AGPTALGFKELGPYSSKTRGVEWKRPSELV#DDPQFIVGGATRTDICQGALGDCWLLAAIG 115
Xenopus laevis	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \| \|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|++\|\|\|\|+\|\| # \|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 56 ATPSSLGFKELGPGSSKTRGVQWKRPSEIV#DDPQFIVGGATRTDICQGALGDCWLLAAIG 115
Coturnix coturnix	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \| \|++\|\|\|\|\|\|\|\|+\|\|\|\|\|\|+\|\|\|\|\|+\|+ # \|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 56 AGPTALGFKELGPHSSKTRGVEWKRPSELV#DDPQFIVGGATRTDICQGALGDCWLLAAIG 115
Danio rerio	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \| \| \|\|\|\|\|\|\| \|+\|\|\|\|\| +\|\| \|\|\|\|+\|# \|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 56 AEPPSLGFKELAPHSSKTRDVEWMRPTELC#DDPQFIVGGATRTDICQGALGDCWLLAAIG 115
Xenopus laevis	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \|+\|\| \|\|\|\|\|\|\|\| \|\|\|\|\|\|++\|\|\|\|++\| # \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 56 AIPSILGFKELGPGSSKTRGVQWKRPSDIV#DDPQFIIGGATRTDICQGALGDCWLLAAIG 115
Bos taurus	Query 54 LPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 +\| \|\|\|\|\|\|\|\|\| \|\|\|\| \|\|+\|\|\|\|\|\|+ #++\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 64 VPQSLGFKELGPNSSKTYGIKWKRPTELF#SNPQFIVDGATRTDICQGALGDCWLLAAIA 122
Gallus gallus	Query 56 SSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|+ \|\|\|\|\|\|+\|# \|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 66 SSLGFRELGPGSSKTRGVTWKRPTELC#RHPQFIVDGATRTDICQGALGDCWLLAAIA 122
Sus scrofa	Query 54 LPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 +\| \|\|\|\|\|\|\|\|\| \|\|\|\| \|++\|\|\|\|\|\|+ #++\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 64 VPQSLGFKELGPNSSKTYGVKWKRPTELF#SNPQFIVDGATRTDICQGALGDCWLLAAIA 122
Canis familiaris	Query 54 LPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 +\| \|\|\|+\|+\|\|\| \|\|\|\| \|\|+\|\|\|\|\|\|+ #++\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 64 VPQSLGYKDLGPNSSKTYGIKWKRPTELL#SNPQFIVDGATRTDICQGALGDCWLLAAIA 122
Oncorhynchus mykiss	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \| \| \|\|\|\| \|\|\|\| \| \|\|\| ++\|\|\|\| \|+\|#+ \|+\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 53 AAPESLGFNELGPGSYKTRAVQWKRPGELC#SSPEFIVGGATRTDICQGALGDCWLLAAIA 112
Rattus norvegicus	Query 57 SLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \|\|\|\|\|\|\|\|\| \|\|\|\| \|\|+\|\|\|\|\|\|+ #++\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 67 SLGFKELGPNSSKTYGIKWKRPTELL#SNPQFIVDGATRTDICQGALGDCWLLAAIA 122
Pongo pygmaeus	Query 54 LPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 +\| \|\|\|+\|+\|\|\| \|\|\|\| \|\|+\|\|\|\|\|\|+ #++\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 64 VPQSLGYKDLGPNSSKTYGIKWKRPTELL#SNPQFIVDGATRTDICQGALGDCWLLAAIA 122
synthetic construct	Query 54 LPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 +\| \|\|\|+\|+\|\|\| \|\|\|\| \|\|+\|\|\|\|\|\|+ #++\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 64 VPQSLGYKDLGPNSSKTYGIKWKRPTELL#SNPQFIVDGATRTDICQGALGDCWLLAAIA 122
Xenopus tropicalis	Query 56 SSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \|\|\|\|\|\|\|\|\|\| +\|\|\|+\|++\|\|\|\| +\| # \|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 59 SSLGFKELGPGTSKTKGVQWKRPHDIA#DDPQFIVGGATRTDICQGALGDCWLLAAIG 115
Danio rerio	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \| \|\|\|\|\| \|\|\|\| \|\|\|\|+\|+ \| \|\| \|+\|#\| \|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 58 ASSSSLGFNELGPRSSKTQGVRWMRPKEMC#ARPQFIVDGATRTDICQGALGDCWLLAAIA 117
Stizostedion vitreum vitreum	Query 56 SSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \|\|\|\|\|\|\|\|\| \| \| \|\|+ \|+\|\|\|\| #+\|\|\|\|\|+ \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 56 SSLGFKELGVGSYKIRGVSWERPTEFT#SDPQFIVSGASRTDICQGALGDCWLLAAIA 112
Oryctolagus cuniculus	Query 57 SLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \|\|\| \|\| +\| + \| \|\|\|\| \|\|\|# +\|+\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\| Sbjct 62 SLGAHELSQ-KTKMKAITWKRPQEIC#ENPRFIIGGANRTDICQGDLGDCWFLAAIA 116
Canis familiaris	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \| \|\|\|\|\|\|\|+\|\|\| \| + \| \|+\|\| +\| #+ \| \|\|+ \| + \|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 135 AEPSSLGFKDLGPNSRNVQNICWQRPGQIT#SSPHFIVNGFSPTDICQGVLGDCWLLAAIG 194
Oncorhynchus mykiss	Query 53 ALPSSLGFKELGPYSSKTRGIEWKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \| \|\|\| + + \| + \|\|\|\|\| \|+\|# +\|+\|\|+\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 36 ACDSSLYYSQSVPIN-----FEWKRPGELC#ENPEFIVGGADRTDICQGALGDCWLLAAIA 90
Ovis aries	Query 75 WKRPTEIC#ADPQFIIGGATRTDICQGALGDCWLLAAIA 112 \|\|\|\| \|\|\|# +\|+\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\| Sbjct 99 WKRPPEIC#ENPRFIIGGANRTDICQGDLGDCWFLAAIA 136

#	organism	max score	hits	top seq
1	Rattus norvegicus	133.00	12	calpain 2
2	Homo sapiens	132.00	11	CAPN2 protein
3	N/A	131.00	14	neutral protease large subunit
4	Macaca mulatta	131.00	2	PREDICTED: calpain 2, large subunit
5	Homo sapiens	131.00	2	calpain 2, large subunit
6	Mus musculus	130.00	20	unnamed protein product
7	Xenopus tropicalis	127.00	4	calpain 2, (m/II) large subunit
8	Tetraodon nigroviridis	119.00	8	unnamed protein product
9	Danio rerio	119.00	5	calpain 2, (m/II) large subunit a
10	Gallus gallus	119.00	3	calpain 2, (m/II) large subunit
11	Oncorhynchus mykiss	119.00	1	m-calpain
12	Xenopus laevis	116.00	1	mu/m-calpain large subunit
13	Coturnix coturnix	116.00	1	quail calpain
14	Canis familiaris	114.00	23	PREDICTED: similar to Calpain-3 (Calpain L3) (Cal
15	Xenopus laevis	114.00	4	hypothetical protein LOC398772
16	Ovis aries	114.00	1	skeletal muscle-specific calpain
17	Rattus norvegicus	114.00	1	calpain 1, large subunit
18	Sus scrofa	113.00	3	calpain 3
19	Bos taurus	112.00	4	calpain 3
20	Oryctolagus cuniculus	112.00	1	calpain 3, (p94)
21	Oncorhynchus mykiss	112.00	1	gill-specific calpain
22	Gallus gallus	110.00	1	mu-calpain large subunit
23	Macaca fascicularis	109.00	1	CAN3_MACFA Calpain-3 (Calpain L3) (Calpain p94) (
24	Stizostedion vitreum vitreum	109.00	1	putative calpain-like protein
25	Pongo pygmaeus	109.00	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
26	synthetic construct	109.00	1	Homo sapiens calpain 1, (mu/I) large subunit
27	Danio rerio	107.00	1	calpain 1, large subunit
28	Canis familiaris	98.20	1	PREDICTED: similar to Calpain-11 (Calcium-activat

#	organism	max score	hits	top seq
1	Mus musculus	126.00	23	calpain 2
2	N/A	126.00	14	A Chain A, Crystal Structure Of M-Calpain
3	Rattus norvegicus	126.00	13	calpain 2
4	Homo sapiens	124.00	11	AF261089_1 calpain large polypeptide L2
5	Homo sapiens	124.00	2	calpain 2, large subunit
6	Macaca mulatta	124.00	2	PREDICTED: calpain 2, large subunit
7	Xenopus tropicalis	115.00	4	calpain 2, (m/II) large subunit
8	Oncorhynchus mykiss	115.00	1	m-calpain
9	Gallus gallus	114.00	2	calpain 2, (m/II) large subunit
10	Tetraodon nigroviridis	112.00	7	unnamed protein product
11	Gallus gallus	112.00	1	mu-calpain large subunit
12	Danio rerio	108.00	5	calpain 2, (m/II) large subunit a
13	Stizostedion vitreum vitreum	107.00	1	putative calpain-like protein
14	synthetic construct	106.00	1	Homo sapiens calpain 1, (mu/I) large subunit
15	Pongo pygmaeus	106.00	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
16	Canis familiaris	105.00	12	PREDICTED: similar to Calpain-1 catalytic subunit
17	Xenopus laevis	105.00	4	calpain
18	Bos taurus	104.00	4	micromolar calcium activated neutral protease 1
19	Sus scrofa	103.00	3	calpain 1, large subunit
20	Danio rerio	102.00	1	calpain 1, large subunit
21	Rattus norvegicus	99.80	1	calpain 1, large subunit
22	Xenopus laevis	98.60	1	mu/m-calpain large subunit
23	Coturnix coturnix	98.60	1	quail calpain
24	Drosophila melanogaster	92.00	3	Calpain-A CG7563-PA, isoform A
25	Drosophila pseudoobscura	91.70	1	GA20829-PA
26	Drosophila melanogaster	90.50	5	CAA55297.1
27	Canis familiaris	90.50	1	PREDICTED: similar to Calpain-11 (Calcium-activat
28	Macaca fascicularis	85.10	1	CAN3_MACFA Calpain-3 (Calpain L3) (Calpain p94) (
29	Oryctolagus cuniculus	85.10	1	calpain 3, (p94)
30	Oncorhynchus mykiss	84.00	1	gill-specific calpain

organism	matching
Mus musculus	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239
N/A	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239
Rattus norvegicus	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239
Homo sapiens	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELKKPPPNLFKIIQKALQKGSLLG 239
Homo sapiens	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELKKPPPNLFKIIQKALQKGSLLG 239
Macaca mulatta	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELKKPPPNLFKIIQKALQKGSLLG 239
Gallus gallus	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 180 LLEKAYAKLNGSYEALSGGTTTEGFEDFTG#GIAEWYELQKAPPNLFKIIQKALQKGSLLG 239
Gallus gallus	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|#\|+ \|\|\|+\|\|\|\|\| +\|++\|\| \|\|\|\|+\|\|\|\|\| Sbjct 190 LLEKAYAKVNGCYEALSGGSTSEGFEDFTG#GVTEWYDLRKPPADLYQIILKALERGSLLG 249
Tetraodon nigroviridis	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|#\|\|\|\| \|+\|\|\|\|\| \|\|\|+\|++\|\|\|\| \|+\|\|\| Sbjct 180 LLEKAYAKVNGCYEALSGGSTTEGFEDFTG#GIAENYDLRKPPSNLFQILKKALEAGALLG 239
Xenopus tropicalis	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|+\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|#\|+\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 180 LMEKAYAKLNGSYEALSGGTTTEGFEDFTG#GLAEWYELRKAPPNLFKIIQKALRTGSLLG 239
Oncorhynchus mykiss	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|#\| \|\| \|+\|\|\|\|\|\|\|+\|+\|\|+\|\|+\| \|+\|\|\| Sbjct 180 LLEKAYAKVNGCYEALSGGSTTEGFEDFTG#GNAENYDLRKPPPNMFQIIKKAIESGALLG 239
Canis familiaris	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|#\|+ \|\|\|\|\|\|\| \| +\|++\|\| \|\|\|\|+\|\|\|\|\| Sbjct 190 LLEKAYAKVNGSYEALSGGSTSEGFEDFTG#GVTEWYELRKAPSDLYQIILKALERGSLLG 249
Danio rerio	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|#\|\|\|\| \|\|\|+ \| \|\|\|+\|\|+\|\|\|+ \|+\|\|\| Sbjct 180 LLEKAYAKVNGCYEALSGGSTTEGFEDFTG#GIAEMYELKSAPTNLFQIIKKALDSGALLG 239
Xenopus laevis	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|#\|+ \|\|\|\|++\| \| +\|\| \|\| \|\|\|\|+\|\|\|+\| Sbjct 190 LLEKAYAKANGCYEALSGGSTSEGFEDFTG#GVTEWYEMKKAPKDLFNIILKALERGSLMG 249
Pongo pygmaeus	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|#\|+ \|\|\|\|\|\|\| \| +\|++\|\| \|\|\|\|+\|\|\|\|\| Sbjct 190 LLEKAYAKVNGSYEALSGGSTSEGFEDFTG#GVTEWYELRKAPSDLYQIILKALERGSLLG 249
synthetic construct	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|#\|+ \|\|\|\|\|\|\| \| +\|++\|\| \|\|\|\|+\|\|\|\|\| Sbjct 190 LLEKAYAKVNGSYEALSGGSTSEGFEDFTG#GVTEWYELRKAPSDLYQIILKALERGSLLG 249
Bos taurus	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|#\|+ \|\|\|\|\|\|\| \| +\|+ \|\| \|\|\|\|+\|\|\|\|\| Sbjct 190 LLEKAYAKVNGSYEALSGGSTSEGFEDFTG#GVTEWYELRKAPSDLYNIILKALERGSLLG 249
Sus scrofa	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|#\|+ \|\|\|\|\|\|\| \| +\|+ \|\| \|\|\|\|+\|\|\|\|\| Sbjct 190 LLEKAYAKVNGSYEALSGGSTSEGFEDFTG#GVTEWYELRKAPSDLYSIILKALERGSLLG 249
Stizostedion vitreum vitreum	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|#\|\|\|\| \|\|\|++\| \|\| \|\|\|\|\| +\|\|\|+\| Sbjct 181 LLEKAYAKLNGCYEALSGGSTTEGFEDFTG#GIAEVYELKQPDARLFHIIQKAQSRGSLMG 240
Rattus norvegicus	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\| \|+\| \|\|\|\|\|\|#\|+ \|\|\|+\|+\| \| +\|++\|\| \|\|\|\|+\|\|\|\|\| Sbjct 190 LLEKAYAKVNGSYEALSGGCTSEAFEDFTG#GVTEWYDLQKAPSDLYQIILKALERGSLLG 249
Coturnix coturnix	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\|\|\|\|+\|\|\|\|+\|\|\|\|\|\|\|\|\|\|#\|+\|\| \|+\|++ \| \|+ \|\|+\|\|\|\|+\|\|\|\|\| Sbjct 183 LLEKAYAKLNGCYESLSGGSTTEGFEDFTG#GVAEMYDLKRAPRNMGHIIRKALERGSLLG 242
Danio rerio	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|#\|+ \| \|\|\|+\| \| +\|\| \|\| +\|+\|+\|\|\|\|\| Sbjct 185 LLEKAYAKLNGCYEALSGGSTCEGFEDFTG#GVTEMYELKKAPADLFSIIGRAIERGSLLG 244
Xenopus laevis	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\| \|\|+\|\|\|\|+\|\|\|\|\|\|\|\|\|\|#\|+\|\| \|\|\|\|\| \| +\| \|\|\|++\|\|\|+\|\|\|\|\| Sbjct 183 LLEKAYAKLNGSYESLSGGSTTEGFEDFTG#GVAEMYELRKAPRDLDKIIKRALERGSLLG 242
Drosophila pseudoobscura	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|++\| \|\|\|\| \|\| \| \| \|\|\|\|\|#\|+ \|\|\|++++ \|\|\|\|\| \|+ \|\| \|+\|\|++\| Sbjct 395 LLEKAYAKLHGSYEALKGGTTCEAMEDFTG#GVTEWYDIKEAPPNLFSIMMKAAERGSMMG 454
Drosophila melanogaster	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|++\| \|\|\|\| \|\| \| \| \|\|\|\|\|#\|+ \|\|\|++++ \|\|\|\|\| \|+ \|\| \|+\|\|++\| Sbjct 389 LLEKAYAKLHGSYEALKGGTTCEAMEDFTG#GVTEWYDIKEAPPNLFSIMMKAAERGSMMG 448
Drosophila melanogaster	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|++\| \|\|\|\| \|\|+\| \| \|\|\|\|\|#\|++\|\|\|+\|++ \| \|\|\| \|+\|\|\| \|+ \|++\| Sbjct 195 LLEKAYAKLHGSYEALKGGSTCEAMEDFTG#GVSEWYDLKEAPGNLFTILQKAAERNSMMG 254
Canis familiaris	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|+\|\| \|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|#\|+ +\|+\|\|\| \|\| ++++\|\|+\|+ \|\|+\| Sbjct 262 LLEKAYAKLNGSYEALSGGSTVEGFEDFTG#GVTYTIQLQKPPRNLLRMLRKAVERSSLMG 321
Macaca fascicularis	Query 180 LLEKAYAKINGCYEALSGGATTEGFEDFTG#GIAEWYELRKPPPNLFKIIQKALEKGSLLG 239 \|\|\|\|\|\|\|\|++\| \|\|\|\| \|\| \|\|\| \|\|\|\|\|#\|+ \|++\|+\| \| ++ \|\|++\|\|+\|+\|\|\|+\| Sbjct 204 LLEKAYAKLHGSYEALKGGNTTEAMEDFTG#GVTEFFEIRDAPSDMHKIMKKAIERGSLMG 263

#	organism	max score	hits	top seq
1	Mus musculus	125.00	21	m-calpain
2	N/A	125.00	14	A Chain A, Crystal Structure Analysis Of Rat M-Ca
3	Rattus norvegicus	125.00	9	calpain 2
4	Macaca mulatta	122.00	2	PREDICTED: calpain 2, large subunit
5	Homo sapiens	119.00	2	calpain 2, large subunit
6	Homo sapiens	118.00	9	CAPN2 protein
7	Gallus gallus	106.00	2	calpain 2, (m/II) large subunit
8	Xenopus tropicalis	103.00	4	calpain 2, (m/II) large subunit
9	Oncorhynchus mykiss	101.00	1	m-calpain
10	Tetraodon nigroviridis	96.30	6	unnamed protein product
11	Gallus gallus	94.40	1	mu-calpain large subunit
12	Danio rerio	94.00	5	calpain 2, (m/II) large subunit a
13	Xenopus laevis	91.70	3	calpain
14	Danio rerio	89.00	1	calpain 1, large subunit
15	Canis familiaris	88.20	17	PREDICTED: similar to Calpain-1 catalytic subunit
16	Sus scrofa	87.80	3	calpain 1, large subunit
17	Bos taurus	87.80	3	micromolar calcium activated neutral protease 1
18	synthetic construct	87.80	1	Homo sapiens calpain 1, (mu/I) large subunit
19	Pongo pygmaeus	87.80	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
20	Xenopus laevis	85.90	1	mu/m-calpain large subunit
21	Stizostedion vitreum vitreum	85.50	1	putative calpain-like protein
22	Coturnix coturnix	80.50	1	quail calpain
23	Oncorhynchus mykiss	78.20	1	gill-specific calpain
24	Rattus norvegicus	75.90	1	calpain 1, large subunit
25	Canis familiaris	67.40	1	PREDICTED: similar to Calpain-11 (Calcium-activat
26	Oryctolagus cuniculus	58.20	1	calpain 3, (p94)
27	Drosophila melanogaster	53.90	5	Calpain, without calmodulin-like domain
28	Drosophila melanogaster	53.90	3	Calpain-A CG7563-PA, isoform A
29	Drosophila pseudoobscura	53.10	1	GA20829-PA
30	Caenorhabditis elegans	50.40	3	CaLPain family member (clp-1)
31	Caenorhabditis briggsae AF16	50.40	1	hypothetical protein CBG22945

organism	matching
Mus musculus	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSGS 277 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSGS 277
N/A	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSGS 277 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSGS 277
Rattus norvegicus	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSGS 277 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSGS 277
Macaca mulatta	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSGS 277 +\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 218 KKPPPNLFKIIQKALQKGSLLGCSIDITSA#ADSEAITYQKLVKGHAYSVTGAEEVESSGS 277
Homo sapiens	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSGS 277 +\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| Sbjct 218 KKPPPNLFKIIQKALQKGSLLGCSIDITSA#ADSEAITFQKLVKGHAYSVTGAEEVESNGS 277
Homo sapiens	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSGS 277 +\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| Sbjct 218 KKPPPNLFKIIQKALQKGSLLGCSIDITSA#ADSEAITFQKLVKGHAYSVTGAEEVESNGS 277
Gallus gallus	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSGS 277 +\| \|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|++\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\| Sbjct 218 QKAPPNLFKIIQKALQKGSLLGCSIDITSA#AETEAVTSQKLVKGHAYSVTGAEEVNFRGS 277
Xenopus tropicalis	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSGS 277 \|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|#\|++\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|+ \|+ Sbjct 218 RKAPPNLFKIIQKALRTGSLLGCSIDITSA#AETEAITFQKLVKGHAYSVTAAEEVQYRGN 277
Oncorhynchus mykiss	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|\|\|\|\|\|+\|+\|\|+\|\|+\| \|+\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|+\|\|\| \|\| \| Sbjct 218 RKPPPNMFQIIKKAIESGALLGCSIDITSA#ADSEAVTRQKLVKGHAYSLTGAVEVTYRG 276
Tetraodon nigroviridis	Query 222 PNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|+\|\| \|\|+\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|#+\|\|\|\|+\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|++\|\| \| Sbjct 228 PHLFHIIKKALERGSLLGCSIDITSA#SDSEAITYRKLVKGHAYSVTGADQVEYRG 282
Gallus gallus	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|\|\|\| +\|++\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|# \| \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|+++ \| Sbjct 228 RKPPADLYQIILKALERGSLLGCSIDITSA#FDMEAVTFKKLVKGHAYSVTGAKQISYRG 286
Danio rerio	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEV 272 + \| \|\|\|+\|\|+\|\|\|+ \|+\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\| Sbjct 218 KSAPTNLFQIIKKALDSGALLGCSIDITSA#ADSEAVTYQKLVKGHAYSLTGATEV 272
Xenopus laevis	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 +\| \|\|\|\|\|+\|\|\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|+\|# \|+\|\|+\| +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 218 KKAPPNLFQIIQKALKAESLLGCSIDITNA#YDTEAITSRKLVKGHAYSVTGAEEVLYRG 276
Danio rerio	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSGS 277 +\| \| +\|\| \|\| +\|+\|+\|\|\|\|\|\|\|\|\|\|\|\| # \| \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|+ Sbjct 223 KKAPADLFSIIGRAIERGSLLGCSIDITSK#FDMEAVTFKKLVKGHAYSVTGAEEVVYRGN 282
Canis familiaris	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|\| \| +\|++\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| # \| \|\|+\|++\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| Sbjct 228 RKAPSDLYQIILKALERGSLLGCSIDISSV#LDMEAITFKKLVKGHAYSVTGAKQVNYQG 286
Sus scrofa	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|\| \| +\|+ \|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| # \| \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| Sbjct 228 RKAPSDLYSIILKALERGSLLGCSIDISSV#LDMEAVTFKKLVKGHAYSVTGAKQVNYQG 286
Bos taurus	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|\| \| +\|+ \|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| # \| \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| Sbjct 228 RKAPSDLYNIILKALERGSLLGCSIDISSI#LDMEAVTFKKLVKGHAYSVTGAKQVNYQG 286
synthetic construct	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|\| \| +\|++\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| # \| \|\|+\|++\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| Sbjct 228 RKAPSDLYQIILKALERGSLLGCSIDISSV#LDMEAITFKKLVKGHAYSVTGAKQVNYRG 286
Pongo pygmaeus	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|\| \| +\|++\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| # \| \|\|+\|++\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| Sbjct 228 RKAPSDLYQIILKALERGSLLGCSIDISSV#LDMEAITFKKLVKGHAYSVTGAKQVNYRG 286
Xenopus laevis	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|\| \| +\| \|\|\|++\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|# \| \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\| +\|\| \| Sbjct 221 RKAPRDLDKIIKRALERGSLLGCSIDITSA#FDMEAVTFKKLVKGHAYSVTALKEVNYMG 279
Stizostedion vitreum vitreum	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 ++\| \|\| \|\|\|\|\| +\|\|\|+\|\|\|\|\|\|\|\|+#++\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| +\|\| \| Sbjct 219 KQPDARLFHIIQKAQSRGSLMGCSIDITSS#SESEAVTSQKLVKGHAYSVTGTAQVEHQG 277
Coturnix coturnix	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 ++ \| \|+ \|\|+\|\|\|\|+\|\|\|\|\|\| \|\|\|\|\|\|# \| \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\| ++\| \| Sbjct 221 KRAPRNMGHIIRKALERGSLLGCPIDITSA#FDMEAVTFKKLVKGHAYSVTAFKDVNYRG 279
Oncorhynchus mykiss	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 + \| +\|\| \|++\|\| ++\|\|++\|\|\|\|\|\|\|\|+#\|+\|\|\| \| \|\|\|\|\|\|\|\|+\|\| \|\|\| \| Sbjct 196 KSAPTDLFNIMKKAYDRGSMMGCSIDITSS#AESEAQTASGLVKGHAYSITGLEEVNYRG 254
Rattus norvegicus	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 +\| \| +\|++\|\| \|\|\|\|+\|\|\|\|\|\|\|\|+\|+ # \| \|\|+\|++ \|\|+\|\|\|\|\|\|\| \|++\| \| Sbjct 228 QKAPSDLYQIILKALERGSLLGCSINISDI#RDLEAITFKNLVRGHAYSVTDAKQVTYQG 286
Canis familiaris	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 +\|\|\| \|\| ++++\|\|+\|+ \|\|+\|\|\|\|+\| \| #++ \| +\| + \|\|+\|\|\|\|\|\|\| +\|\| \| Sbjct 300 QKPPRNLLRMLRKAVERSSLMGCSIEIVSE#SELETMTQKMLVRGHAYSVTDLQEVWYQG 358
Oryctolagus cuniculus	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVTGAEEVESSG 276 + \| +++\|\|++\|\|+\|+\| \|+\|\|\|\|\| # \| \|\|\|\|\|\|\|\|\|\|\| \|\| \| Sbjct 222 KDAPRDMYKIMRKAIERGFLMGCSIDTIVP#VQYETRMACGLVKGHAYSVTGLEETLFKG 280
Drosophila melanogaster	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVT 267 ++ \| \|\|\| \|+\|\|\| \|+ \|++\|\|\|\|+ # +\|\| \| \| \|++\|\|\|\|\|+\| Sbjct 256 KEAPGNLFTILQKAAERNSMMGCSIEPDPN#V-TEAETPQGLIRGHAYSIT 304
Drosophila melanogaster	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVT 267 ++ \| \|\|\| \|+\|\|\| \|+ \|++\|\|\|\|+ # +\|\| \| \| \|++\|\|\|\|\|+\| Sbjct 256 KEAPGNLFTILQKAAERNSMMGCSIEPDPN#V-TEAETPQGLIRGHAYSIT 304
Drosophila pseudoobscura	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADSEAVTYQKLVKGHAYSVT 267 ++ \|\|\|\|\| \|+ \|\| \|+\|\|++\|\|\|++ # \|\| \| + \|++\|\|\|\|\|+\| Sbjct 433 KEAPPNLFSIMMKAAERGSMMGCSLEPDPH#V-LEAETREGLIRGHAYSIT 481
Caenorhabditis elegans	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADS---EAVTYQKLVKGHAYSVTGAEEVE 273 + \|\| \|\| +++ + \| \|\|\| \|\|\|\|+ #\|\| \|\| \|\|\|\|\|\|\|\|+\|\| \|+ Sbjct 438 KNPPRNLMQMMMRGFEMGSLFGCSIE----#ADPNVWEAKMSNGLVKGHAYSITGCRIVD 492
Caenorhabditis briggsae AF16	Query 218 RKPPPNLFKIIQKALEKGSLLGCSIDITSA#ADS---EAVTYQKLVKGHAYSVTGAEEVE 273 + \|\| \|\| +++ + \| \|\|\| \|\|\|\|+ #\|\| \|\| \|\|\|\|\|\|\|\|+\|\| \|+ Sbjct 433 KNPPRNLMQMMMRGFEMGSLFGCSIE----#ADPNVWEAKMSNGLVKGHAYSITGCRIVD 487

organism	matching
Mus musculus	Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSLQ 279 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSLQ 279
Rattus norvegicus	Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSLQ 279 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSLQ 279
N/A	Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSLQ 279 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSLQ 279
Macaca mulatta	Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSLQ 279 \|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 220 PPPNLFKIIQKALQKGSLLGCSIDITSAAD#SEAITYQKLVKGHAYSVTGAEEVESSGSLQ 279
Homo sapiens	Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSLQ 279 \|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 220 PPPNLFKIIQKALQKGSLLGCSIDITSAAD#SEAITFQKLVKGHAYSVTGAEEVESNGSLQ 279
Homo sapiens	Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSLQ 279 \|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 220 PPPNLFKIIQKALQKGSLLGCSIDITSAAD#SEAITFQKLVKGHAYSVTGAEEVESNGSLQ 279
Gallus gallus	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSLQ 279 \|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+#+\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|+\| Sbjct 221 PPNLFKIIQKALQKGSLLGCSIDITSAAE#TEAVTSQKLVKGHAYSVTGAEEVNFRGSIQ 279
Xenopus tropicalis	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSLQ 279 \|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|+#+\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|+ \|+\|+ Sbjct 221 PPNLFKIIQKALRTGSLLGCSIDITSAAE#TEAITFQKLVKGHAYSVTAAEEVQYRGNLE 279
Oncorhynchus mykiss	Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|\|\|\|+\|+\|\|+\|\|+\| \|+\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|+\|\|\| \|\| \| Sbjct 220 PPPNMFQIIKKAIESGALLGCSIDITSAAD#SEAVTRQKLVKGHAYSLTGAVEVTYRG 276
Tetraodon nigroviridis	Query 222 PNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|+\|\| \|\|+\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|+\|#\|\|\|+\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|++\|\| \| Sbjct 228 PHLFHIIKKALERGSLLGCSIDITSASD#SEAITYRKLVKGHAYSVTGADQVEYRG 282
Danio rerio	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEV 272 \| \|\|\|+\|\|+\|\|\|+ \|+\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\| Sbjct 221 PTNLFQIIKKALDSGALLGCSIDITSAAD#SEAVTYQKLVKGHAYSLTGATEV 272
Gallus gallus	Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|\| +\|++\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|# \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|+++ \| Sbjct 230 PPADLYQIILKALERGSLLGCSIDITSAFD#MEAVTFKKLVKGHAYSVTGAKQISYRG 286
Xenopus laevis	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|\|\|\|\|+\|\|\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|+\| \|#+\|\|+\| +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 221 PPNLFQIIQKALKAESLLGCSIDITNAYD#TEAITSRKLVKGHAYSVTGAEEVLYRG 276
Danio rerio	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSL 278 \| +\|\| \|\| +\|+\|+\|\|\|\|\|\|\|\|\|\|\|\| \|# \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|++ Sbjct 226 PADLFSIIGRAIERGSLLGCSIDITSKFD#MEAVTFKKLVKGHAYSVTGAEEVVYRGNM 283
Canis familiaris	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSL 278 \| +\|++\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| \|# \|\|+\|++\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| + Sbjct 231 PSDLYQIILKALERGSLLGCSIDISSVLD#MEAITFKKLVKGHAYSVTGAKQVNYQGQM 288
Sus scrofa	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSL 278 \| +\|+ \|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| \|# \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| + Sbjct 231 PSDLYSIILKALERGSLLGCSIDISSVLD#MEAVTFKKLVKGHAYSVTGAKQVNYQGQM 288
Bos taurus	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSL 278 \| +\|+ \|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| \|# \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| + Sbjct 231 PSDLYNIILKALERGSLLGCSIDISSILD#MEAVTFKKLVKGHAYSVTGAKQVNYQGQM 288
Pongo pygmaeus	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSL 278 \| +\|++\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| \|# \|\|+\|++\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| + Sbjct 231 PSDLYQIILKALERGSLLGCSIDISSVLD#MEAITFKKLVKGHAYSVTGAKQVNYRGQM 288
synthetic construct	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSL 278 \| +\|++\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| \|# \|\|+\|++\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| + Sbjct 231 PSDLYQIILKALERGSLLGCSIDISSVLD#MEAITFKKLVKGHAYSVTGAKQVNYRGQV 288
Stizostedion vitreum vitreum	Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSLQ 279 \| \|\| \|\|\|\|\| +\|\|\|+\|\|\|\|\|\|\|\|+++#\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| +\|\| \| + Sbjct 221 PDARLFHIIQKAQSRGSLMGCSIDITSSSE#SEAVTSQKLVKGHAYSVTGTAQVEHQGGTE 280
Xenopus laevis	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSGSLQ 279 \| +\| \|\|\|++\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|# \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\| +\|\| \| ++ Sbjct 224 PRDLDKIIKRALERGSLLGCSIDITSAFD#MEAVTFKKLVKGHAYSVTALKEVNYMGEME 282
Coturnix coturnix	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSG 276 \| \|+ \|\|+\|\|\|\|+\|\|\|\|\|\| \|\|\|\|\|\| \|# \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\| ++\| \| Sbjct 224 PRNMGHIIRKALERGSLLGCPIDITSAFD#MEAVTFKKLVKGHAYSVTAFKDVNYRG 279
Oncorhynchus mykiss	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSG 276 \| +\|\| \|++\|\| ++\|\|++\|\|\|\|\|\|\|\|+\|+#\|\|\| \| \|\|\|\|\|\|\|\|+\|\| \|\|\| \| Sbjct 199 PTDLFNIMKKAYDRGSMMGCSIDITSSAE#SEAQTASGLVKGHAYSITGLEEVNYRG 254
Rattus norvegicus	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSG 276 \| +\|++\|\| \|\|\|\|+\|\|\|\|\|\|\|\|+\|+ \|# \|\|+\|++ \|\|+\|\|\|\|\|\|\| \|++\| \| Sbjct 231 PSDLYQIILKALERGSLLGCSINISDIRD#LEAITFKNLVRGHAYSVTDAKQVTYQG 286
Canis familiaris	Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSG 276 \|\| \|\| ++++\|\|+\|+ \|\|+\|\|\|\|+\| \| ++# \| +\| + \|\|+\|\|\|\|\|\|\| +\|\| \| Sbjct 302 PPRNLLRMLRKAVERSSLMGCSIEIVSESE#LETMTQKMLVRGHAYSVTDLQEVWYQG 358
Oryctolagus cuniculus	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVTGAEEVESSG 276 \| +++\|\|++\|\|+\|+\| \|+\|\|\|\|\| # \| \|\|\|\|\|\|\|\|\|\|\| \|\| \| Sbjct 225 PRDMYKIMRKAIERGFLMGCSIDTIVPVQ#YETRMACGLVKGHAYSVTGLEETLFKG 280
Drosophila melanogaster	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVT 267 \|\|\|\|\| \|+ \|\| \|+\|\|++\|\|\|++ # \|\| \| \| \|++\|\|\|\|\|+\| Sbjct 430 PPNLFSIMMKAAERGSMMGCSLEPDPHV-#LEAETPQGLIRGHAYSIT 475
Drosophila melanogaster	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVT 267 \| \|\|\| \|+\|\|\| \|+ \|++\|\|\|\|+ #+\|\| \| \| \|++\|\|\|\|\|+\| Sbjct 236 PGNLFTILQKAAERNSMMGCSIEPDPNV-#TEAETPQGLIRGHAYSIT 281
Drosophila pseudoobscura	Query 221 PPNLFKIIQKALEKGSLLGCSIDITSAAD#SEAVTYQKLVKGHAYSVT 267 \|\|\|\|\| \|+ \|\| \|+\|\|++\|\|\|++ # \|\| \| + \|++\|\|\|\|\|+\| Sbjct 436 PPNLFSIMMKAAERGSMMGCSLEPDPHV-#LEAETREGLIRGHAYSIT 481
Caenorhabditis elegans	Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#S---EAVTYQKLVKGHAYSVTGAEEVE 273 \|\| \|\| +++ + \| \|\|\| \|\|\|\|+ \|\|# \|\| \|\|\|\|\|\|\|\|+\|\| \|+ Sbjct 440 PPRNLMQMMMRGFEMGSLFGCSIE----AD#PNVWEAKMSNGLVKGHAYSITGCRIVD 492
Caenorhabditis briggsae AF16	Query 220 PPPNLFKIIQKALEKGSLLGCSIDITSAAD#S---EAVTYQKLVKGHAYSVTGAEEVE 273 \|\| \|\| +++ + \| \|\|\| \|\|\|\|+ \|\|# \|\| \|\|\|\|\|\|\|\|+\|\| \|+ Sbjct 435 PPRNLMQMMMRGFEMGSLFGCSIE----AD#PNVWEAKMSNGLVKGHAYSITGCRIVD 487

#	organism	max score	hits	top seq
1	Mus musculus	121.00	21	AF497625_1 m-calpain 80 kDa large subunit
2	N/A	120.00	14	A Chain A, Crystal Structure Of M-Calpain
3	Rattus norvegicus	120.00	9	calpain 2
4	Macaca mulatta	118.00	2	PREDICTED: calpain 2, large subunit
5	Homo sapiens	116.00	9	CAPN2 protein
6	Homo sapiens	116.00	2	calpain 2, large subunit
7	Gallus gallus	105.00	2	calpain 2, (m/II) large subunit
8	Xenopus tropicalis	100.00	4	calpain 2, (m/II) large subunit
9	Tetraodon nigroviridis	96.30	7	unnamed protein product
10	Oncorhynchus mykiss	93.60	1	m-calpain
11	Danio rerio	93.20	5	calpain 2, (m/II) large subunit a
12	Xenopus laevis	93.20	3	hypothetical protein LOC398772
13	Xenopus laevis	92.00	1	mu/m-calpain large subunit
14	Danio rerio	90.10	1	calpain 1, large subunit
15	Stizostedion vitreum vitreum	88.60	1	putative calpain-like protein
16	Gallus gallus	86.70	1	mu-calpain large subunit
17	Canis familiaris	86.30	17	PREDICTED: similar to Calpain-1 catalytic subunit
18	Sus scrofa	86.30	3	calpain 1, large subunit
19	Pongo pygmaeus	86.30	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
20	Bos taurus	85.90	4	micromolar calcium activated neutral protease 1
21	synthetic construct	85.50	1	Homo sapiens calpain 1, (mu/I) large subunit
22	Coturnix coturnix	85.10	1	quail calpain
23	Oncorhynchus mykiss	79.70	1	gill-specific calpain
24	Rattus norvegicus	73.90	1	calpain 1, large subunit
25	Canis familiaris	65.90	1	PREDICTED: similar to Calpain-11 (Calcium-activat
26	Oryctolagus cuniculus	61.20	1	calpain 3, (p94)
27	Caenorhabditis elegans	50.80	7	CaLPain family member (clp-6)
28	Caenorhabditis briggsae AF16	45.40	2	Hypothetical protein CBG13923
29	Drosophila melanogaster	44.30	4	CAA55297.1
30	Drosophila melanogaster	44.30	2	Calpain-A CG7563-PB, isoform B

organism	matching
Mus musculus	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285
N/A	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285
Rattus norvegicus	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285
Macaca mulatta	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 226 KIIQKALQKGSLLGCSIDITSAADSEAITY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285
Homo sapiens	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|+#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\| Sbjct 226 KIIQKALQKGSLLGCSIDITSAADSEAITF#QKLVKGHAYSVTGAEEVESNGSLQKLIRIR 285
Homo sapiens	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|+#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\| Sbjct 226 KIIQKALQKGSLLGCSIDITSAADSEAITF#QKLVKGHAYSVTGAEEVESNGSLQKLIRIR 285
Gallus gallus	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|++\|\|\|\| #\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\| Sbjct 226 KIIQKALQKGSLLGCSIDITSAAETEAVTS#QKLVKGHAYSVTGAEEVNFRGSIQKLIRIR 285
Xenopus tropicalis	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|++\|\|+\|+#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|+ \|+\|+\|\|\|\|\|\| Sbjct 226 KIIQKALRTGSLLGCSIDITSAAETEAITF#QKLVKGHAYSVTAAEEVQYRGNLEKLIRIR 285
Tetraodon nigroviridis	Query 227 IIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|+\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|+\|\|#+\|\|\|\|\|\|\|\|\|\|\|\|\|++\|\| \| +\|+\|\|\| Sbjct 233 IIKKALERGSLLGCSIDITSASDSEAITY#RKLVKGHAYSVTGADQVEYRGEAVQLVRIR 291
Oncorhynchus mykiss	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 +\|\|+\|\|+\| \|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| #\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\| \| +\|\|\|\|\|\| Sbjct 226 QIIKKAIESGALLGCSIDITSAADSEAVTR#QKLVKGHAYSLTGAVEVTYRGRKEKLIRIR 285
Danio rerio	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 +\|\|+\|\|\|+ \|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\| +\|\|+\|+\| Sbjct 226 QIIKKALDSGALLGCSIDITSAADSEAVTY#QKLVKGHAYSLTGATEVNYRSRKEKLVRVR 285
Xenopus laevis	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|\|++\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|\|+#+\|\|\|\|\|\|\|\|\|\|\| +\|\|+ \| ++\|\|\|\|\|\| Sbjct 229 KIIKRALERGSLLGCSIDITSAFDMEAVTF#KKLVKGHAYSVTALKEVDYRGGMEKLIRIR 288
Xenopus laevis	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|\|++\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|\|+#+\|\|\|\|\|\|\|\|\|\|\| +\|\| \| ++\|\|\|\|\|\| Sbjct 229 KIIKRALERGSLLGCSIDITSAFDMEAVTF#KKLVKGHAYSVTALKEVNYMGEMEKLIRIR 288
Danio rerio	Query 227 IIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\| +\|+\|+\|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|\|+#+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|++ \|\|+\|\|\| Sbjct 232 IIGRAIERGSLLGCSIDITSKFDMEAVTF#KKLVKGHAYSVTGAEEVVYRGNMTKLVRIR 290
Stizostedion vitreum vitreum	Query 227 IIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|\|\|\| +\|\|\|+\|\|\|\|\|\|\|\|+++\|\|\|\|\| #\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\| \| +\|\|\|\|\|\| Sbjct 228 IIQKAQSRGSLMGCSIDITSSSESEAVTS#QKLVKGHAYSVTGTAQVEHQGGTEKLIRIR 286
Gallus gallus	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 +\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|\|+#+\|\|\|\|\|\|\|\|\|\|\|\|\|+++ \| \|\|\|+\| Sbjct 236 QIILKALERGSLLGCSIDITSAFDMEAVTF#KKLVKGHAYSVTGAKQISYRGQSLGLIRMR 295
Canis familiaris	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 +\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| \| \|\|+\|+#+\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| + \|\|\|+\| Sbjct 236 QIILKALERGSLLGCSIDISSVLDMEAITF#KKLVKGHAYSVTGAKQVNYQGQMVSLIRMR 295
Sus scrofa	Query 227 IIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| \| \|\|\|\|+#+\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| + \|\|\|+\| Sbjct 237 IILKALERGSLLGCSIDISSVLDMEAVTF#KKLVKGHAYSVTGAKQVNYQGQMVNLIRMR 295
Pongo pygmaeus	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 +\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| \| \|\|+\|+#+\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| + \|\|\|+\| Sbjct 236 QIILKALERGSLLGCSIDISSVLDMEAITF#KKLVKGHAYSVTGAKQVNYRGQMVSLIRMR 295
Bos taurus	Query 227 IIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| \| \|\|\|\|+#+\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| + \|\|\|+\| Sbjct 237 IILKALERGSLLGCSIDISSILDMEAVTF#KKLVKGHAYSVTGAKQVNYQGQMVNLIRMR 295
synthetic construct	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 +\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|+\| \| \|\|+\|+#+\|\|\|\|\|\|\|\|\|\|\|\|\|++\| \| + \|\|\|+\| Sbjct 236 QIILKALERGSLLGCSIDISSVLDMEAITF#KKLVKGHAYSVTGAKQVNYRGQVVSLIRMR 295
Coturnix coturnix	Query 227 IIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|+\|\|\|\|+\|\|\|\|\|\| \|\|\|\|\|\| \| \|\|\|\|+#+\|\|\|\|\|\|\|\|\|\|\| ++\| \| ++\|\|\|\|\| Sbjct 230 IIRKALERGSLLGCPIDITSAFDMEAVTF#KKLVKGHAYSVTAFKDVNYRGQQEQLIRIR 288
Oncorhynchus mykiss	Query 227 IIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|++\|\| ++\|\|++\|\|\|\|\|\|\|\|+\|+\|\|\| \| # \|\|\|\|\|\|\|\|+\|\| \|\|\| \| \|\|\|\|\|\| Sbjct 205 IMKKAYDRGSMMGCSIDITSSAESEAQTA#SGLVKGHAYSITGLEEVNYRGKKVKLIRIR 263
Rattus norvegicus	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 +\|\| \|\|\|\|+\|\|\|\|\|\|\|\|+\|+ \| \|\|+\|+#+ \|\|+\|\|\|\|\|\|\| \|++\| \| \|\|\|+\| Sbjct 236 QIILKALERGSLLGCSINISDIRDLEAITF#KNLVRGHAYSVTDAKQVTYQGQRVNLIRMR 295
Canis familiaris	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 ++++\|\|+\|+ \|\|+\|\|\|\|+\| \| ++ \| +\| #+ \|\|+\|\|\|\|\|\|\| +\|\| \| + \|\|\|+\| Sbjct 308 RMLRKAVERSSLMGCSIEIVSESELETMTQ#KMLVRGHAYSVTDLQEVWYQGRTETLIRVR 367
Oryctolagus cuniculus	Query 226 KIIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 \|\|++\|\|+\|+\| \|+\|\|\|\|\| \| # \|\|\|\|\|\|\|\|\|\|\| \|\| \| \|\|+\|+\| Sbjct 230 KIMRKAIERGFLMGCSIDTIVPVQYETRMA#CGLVKGHAYSVTGLEETLFKGEKVKLVRLR 289
Caenorhabditis elegans	Query 227 IIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 +\| \| ++ \|\|\| \|\|\|\| + \|\| # \|\|+\|\|\|\|\|\|\|\| \|\|+ \|+\|\|\| Sbjct 335 MIMKGMQMGSLFACSID-PDPREKEAQLA#NGLVRGHAYSVTGVHTVETDKEKVALLRIR 392
Caenorhabditis briggsae AF16	Query 227 IIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVTGAEEVESSGSLQKLIRIR 285 ++ + ++ \|\|\| \|\|\|\|\| \|\| # \|\|+\|\|\|\|\|+\| + \| + \|+\|\|\| Sbjct 487 MLVRGMQMGSLFGCSIDADENV-KEAQLT#NGLVRGHAYSITAIQTVNTYSGQTPLLRIR 544
Drosophila melanogaster	Query 227 IIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVT 267 \|+\|\|\| \|+ \|++\|\|\|\|+ +\|\| \| #\| \|++\|\|\|\|\|+\| Sbjct 242 ILQKAAERNSMMGCSIEPDPNV-TEAETP#QGLIRGHAYSIT 281
Drosophila melanogaster	Query 227 IIQKALEKGSLLGCSIDITSAADSEAVTY#QKLVKGHAYSVT 267 \|+\|\|\| \|+ \|++\|\|\|\|+ +\|\| \| #\| \|++\|\|\|\|\|+\| Sbjct 265 ILQKAAERNSMMGCSIEPDPNV-TEAETP#QGLIRGHAYSIT 304

organism	matching
Mus musculus	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297
N/A	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297
Rattus norvegicus	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297
Macaca mulatta	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|+\| Sbjct 238 LGCSIDITSAADSEAITYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGEVEWTGRW 297
Homo sapiens	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|+\| Sbjct 238 LGCSIDITSAADSEAITFQKLVKGHAYSVT#GAEEVESNGSLQKLIRIRNPWGEVEWTGRW 297
Homo sapiens	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|+\| Sbjct 238 LGCSIDITSAADSEAITFQKLVKGHAYSVT#GAEEVESNGSLQKLIRIRNPWGEVEWTGRW 297
Gallus gallus	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\|\|++\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\| Sbjct 238 LGCSIDITSAAETEAVTSQKLVKGHAYSVT#GAEEVNFRGSIQKLIRIRNPWGEVEWTGKW 297
Xenopus tropicalis	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\|\|++\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\|# \|\|\|\|+ \|+\|+\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\| \| Sbjct 238 LGCSIDITSAAETEAITFQKLVKGHAYSVT#AAEEVQYRGNLEKLIRIRNPWGEVEWTGAW 297
Danio rerio	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|#\|\| \|\| +\|\|+\|+\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 238 LGCSIDITSAADSEAVTYQKLVKGHAYSLT#GATEVNYRSRKEKLVRVRNPWGQVEWTGAW 297
Oncorhynchus mykiss	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|+\|#\|\| \|\| \| +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 238 LGCSIDITSAADSEAVTRQKLVKGHAYSLT#GAVEVTYRGRKEKLIRIRNPWGQVEWTGAW 297
Tetraodon nigroviridis	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\|+\|\|\|\|+\|\|+\|\|\|\|\|\|\|\|\|\|\|#\|\|++\|\| \| +\|+\|\|\|\|\|\|\|\|\|\|\| \| \| Sbjct 244 LGCSIDITSASDSEAITYRKLVKGHAYSVT#GADQVEYRGEAVQLVRIRNPWGQVEWNGAW 303
Xenopus laevis	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\| \| \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|# +\|\|+ \| ++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 241 LGCSIDITSAFDMEAVTFKKLVKGHAYSVT#ALKEVDYRGGMEKLIRIRNPWGQVEWTGAW 300
Stizostedion vitreum vitreum	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 +\|\|\|\|\|\|\|\|+++\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|#\| +\|\| \| +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 239 MGCSIDITSSSESEAVTSQKLVKGHAYSVT#GTAQVEHQGGTEKLIRIRNPWGQVEWTGAW 298
Xenopus laevis	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\| \| \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|# +\|\| \| ++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 241 LGCSIDITSAFDMEAVTFKKLVKGHAYSVT#ALKEVNYMGEMEKLIRIRNPWGQVEWTGAW 300
Danio rerio	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\| \| \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|++ \|\|+\|\|\|\|\|\|\|+\|\|\|\|\| \| Sbjct 243 LGCSIDITSKFDMEAVTFKKLVKGHAYSVT#GAEEVVYRGNMTKLVRIRNPWGEVEWTGAW 302
Bos taurus	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|+\| \| \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|#\|\|++\| \| + \|\|\|+\|\|\|\|\|+\|\|\|\|\| \| Sbjct 248 LGCSIDISSILDMEAVTFKKLVKGHAYSVT#GAKQVNYQGQMVNLIRMRNPWGEVEWTGAW 307
Canis familiaris	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|+\| \| \|\|+\|++\|\|\|\|\|\|\|\|\|\|\|#\|\|++\| \| + \|\|\|+\|\|\|\|\|+\|\|\|\|\| \| Sbjct 248 LGCSIDISSVLDMEAITFKKLVKGHAYSVT#GAKQVNYQGQMVSLIRMRNPWGEVEWTGAW 307
Gallus gallus	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\| \| \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|#\|\|+++ \| \|\|\|+\|\|\|\|\|+\|\|\|\|\| \| Sbjct 248 LGCSIDITSAFDMEAVTFKKLVKGHAYSVT#GAKQISYRGQSLGLIRMRNPWGEVEWTGAW 307
Pongo pygmaeus	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|+\| \| \|\|+\|++\|\|\|\|\|\|\|\|\|\|\|#\|\|++\| \| + \|\|\|+\|\|\|\|\|+\|\|\|\|\| \| Sbjct 248 LGCSIDISSVLDMEAITFKKLVKGHAYSVT#GAKQVNYRGQMVSLIRMRNPWGEVEWTGAW 307
synthetic construct	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|+\| \| \|\|+\|++\|\|\|\|\|\|\|\|\|\|\|#\|\|++\| \| + \|\|\|+\|\|\|\|\|+\|\|\|\|\| \| Sbjct 248 LGCSIDISSVLDMEAITFKKLVKGHAYSVT#GAKQVNYRGQVVSLIRMRNPWGEVEWTGAW 307
Coturnix coturnix	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\| \|\|\|\|\|\| \| \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|# ++\| \| ++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 241 LGCPIDITSAFDMEAVTFKKLVKGHAYSVT#AFKDVNYRGQQEQLIRIRNPWGQVEWTGAW 300
Sus scrofa	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|+\| \| \|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|#\|\|++\| \| + \|\|\|+\|\|\|\|\|+\|\|\|\|\| \| Sbjct 248 LGCSIDISSVLDMEAVTFKKLVKGHAYSVT#GAKQVNYQGQMVNLIRMRNPWGEVEWTGAW 307
Oncorhynchus mykiss	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 +\|\|\|\|\|\|\|\|+\|+\|\|\| \| \|\|\|\|\|\|\|\|+\|#\| \|\|\| \| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \| \| Sbjct 216 MGCSIDITSSAESEAQTASGLVKGHAYSIT#GLEEVNYRGKKVKLIRIRNPWGQVEWNGAW 275
Rattus norvegicus	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|+\|+ \| \|\|+\|++ \|\|+\|\|\|\|\|\|\|# \|++\| \| \|\|\|+\|\|\|\|\|+\|\|\| \| \| Sbjct 248 LGCSINISDIRDLEAITFKNLVRGHAYSVT#DAKQVTYQGQRVNLIRMRNPWGEVEWKGPW 307
Canis familiaris	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 +\|\|\|\|+\| \| ++ \| +\| + \|\|+\|\|\|\|\|\|\|# +\|\| \| + \|\|\|+\|\|\|\|\|+\|\|\| \| \| Sbjct 320 MGCSIEIVSESELETMTQKMLVRGHAYSVT#DLQEVWYQGRTETLIRVRNPWGRVEWNGAW 379
Oryctolagus cuniculus	Query 238 LGCSIDITSAADSEAVTYQKLVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 +\|\|\|\|\| \| \|\|\|\|\|\|\|\|\|\|#\| \|\| \| \|\|+\|+\|\|\|\|\|\|\|\|\| \| \| Sbjct 242 MGCSIDTIVPVQYETRMACGLVKGHAYSVT#GLEETLFKGEKVKLVRLRNPWGQVEWNGSW 301
Ovis aries	Query 258 LVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\|#\| \|\| \| \|\|+\|+\|\|\|\|\|\|\|\|\| \| \| Sbjct 331 LVKGHAYSVT#GLEEALYKGEKVKLVRLRNPWGQVEWNGSW 370
Bos taurus	Query 258 LVKGHAYSVT#GAEEVESSGSLQKLIRIRNPWGQVEWTGKW 297 \|\|\|\|\|\|\|\|\|\|#\| \|\| \| \|\|+\|+\|\|\|\|\|\|\|\|\| \| \| Sbjct 53 LVKGHAYSVT#GLEEALYKGEKVKLVRLRNPWGQVEWNGSW 92

#	organism	max score	hits	top seq
1	Mus musculus	143.00	16	unnamed protein product
2	N/A	143.00	10	A Chain A, Crystal Structure Of M-Calpain
3	Rattus norvegicus	143.00	9	calpain 2
4	Homo sapiens	137.00	13	AF261089_1 calpain large polypeptide L2
5	Homo sapiens	137.00	2	calpain 2, large subunit
6	Macaca mulatta	137.00	2	PREDICTED: calpain 2, large subunit
7	Bos grunniens	125.00	1	calpain 2
8	Xenopus tropicalis	120.00	4	calpain 2, (m/II) large subunit
9	Gallus gallus	119.00	3	calpain 2, (m/II) large subunit
10	Stizostedion vitreum vitreum	112.00	1	putative calpain-like protein
11	Tetraodon nigroviridis	110.00	6	unnamed protein product
12	Danio rerio	106.00	5	calpain 2, (m/II) large subunit a
13	Xenopus laevis	102.00	3	calpain 1, (mu/I) large subunit
14	Oncorhynchus mykiss	102.00	1	m-calpain
15	Canis familiaris	99.80	22	PREDICTED: similar to Calpain-1 catalytic subunit
16	Gallus gallus	99.80	1	mu-calpain large subunit
17	Xenopus laevis	99.00	1	mu/m-calpain large subunit
18	Pongo pygmaeus	97.80	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
19	synthetic construct	97.80	1	Homo sapiens calpain 1, (mu/I) large subunit
20	Bos taurus	97.40	5	calpain 1, (mu/I) large subunit
21	Sus scrofa	97.10	2	calpain 1, large subunit
22	Coturnix coturnix	96.30	1	quail calpain
23	Danio rerio	94.00	1	calpain 1, large subunit
24	Rattus norvegicus	94.00	1	calpain 1, large subunit
25	Mus musculus	87.40	1	stomach specific calpain nCL-2
26	Oncorhynchus mykiss	86.70	1	gill-specific calpain
27	Drosophila melanogaster	85.10	3	Calpain-B CG8107-PA
28	Macaca fascicularis	85.10	1	CAN3_MACFA Calpain-3 (Calpain L3) (Calpain p94) (
29	Drosophila pseudoobscura	84.70	1	GA20829-PA
30	Drosophila melanogaster	84.30	5	CAA55297.1
31	Oryctolagus cuniculus	83.20	1	calpain 3, (p94)
32	Ovis aries	81.60	2	skeletal muscle-specific calpain

organism	matching
Mus musculus	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389
N/A	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389
Rattus norvegicus	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389
Homo sapiens	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|+\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 330 DFLRHYSRLEICNLTPDTLTSDTYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389
Homo sapiens	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|+\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 330 DFLRHYSRLEICNLTPDTLTSDTYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389
Macaca mulatta	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|+\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 330 DFLRHYSRLEICNLTPDTLTSDTYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389
Bos grunniens	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|+\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 42 DFLRHYSRLEICNLTPDTLTSDTYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 101
Xenopus tropicalis	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| \| \|\| \|\|#\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|+\| Sbjct 330 DFLRNYSRLEICNLTPDTLASDKYSKWSLT#KMDGTWRRGSTAGGCRNFPETFWMNPQYVI 389
Gallus gallus	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|\| \| #\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| Sbjct 330 DFLRHYSRLEICNLTPDTLASDRYKKWSLL#KLDGNWRRGATAGGCRNYPNTFWTNPQYLI 389
Stizostedion vitreum vitreum	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\| \|+ \|+ \|\| \|+#\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\|\|++\| Sbjct 331 DFLRHYDRLEICNLTPDALSDDTVSKWALS#KFDGSWRRGSTAGGCRNYPDTFWTNPQFVI 390
Tetraodon nigroviridis	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\| \| \|\|\|\| \|#+ + \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|+\|\|\|++\| Sbjct 336 DFLRQYSRLEICNLTPDALTGDEYKKWAET#EFEDTWRRGSTAGGCRNYPNSFWLNPQFVI 395
Danio rerio	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|+\| \|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\| \| \| + #\| \|\| \|\|+\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|+ \| Sbjct 328 DFVRQYSRLEICTLTPDTLTSDSLKHWSVC#KFDGTWRKGSTAGGCRNHPYTFWMNPQFKI 387
Xenopus laevis	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\| +\|\|\|\|\|\|\|\|\|\|\| \|\| \|+\|\| \|# +\|+\|\|+\|\|\|\|\|\|\|\|\|+\| \|\|\|+\|\|\|+ \| Sbjct 340 DFLREFSRLEICNLTPDALTARRYRKWNTT#VYNGSWRKGSTAGGCRNFPATFWINPQFKI 399
Oncorhynchus mykiss	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 +\|\|\|\|\|\|\|+\|\|\| \|\|\|\|\|\|\| \|\| \| \| + # \|\|+\|\|+\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\|++\| Sbjct 328 EFLRHYSRIEICTLTPDTLTDDSVKHWSVC#NYDGSWRKGSTAGGCRNHAYTFWMNPQFVI 387
Canis familiaris	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|+\| ++\|\|\|\|\|\|\|\|\|\| \| \| +\|\| \|# +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \| Sbjct 337 DFMREFTRLEICNLTPDALKSRSIRKWNTT#LYEGTWRRGSTAGGCRNYPATFWVNPQFKI 396
Gallus gallus	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\| ++\|\|\|\|\|\|\|\|\|\| \| ++\|\| # \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \| Sbjct 340 DFLREFTRLEICNLTPDALQSRKFRKWNTR#LYDGSWRRGSTAGGCRNYPATFWINPQFKI 399
Xenopus laevis	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 +\|\|\| +\|\|\|\|\|\|\|\|\|\|\|\|\| + \|\| \|# \|\|+\|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|+\|\|\|+ + Sbjct 333 EFLRQFSRLEICNLTPDTLNKEGLSKWHTT#LYDGSWRRGSTAGGCRNNPATFWINPQFKV 392
Pongo pygmaeus	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|+\| ++\|\|\|\|\|\|\|\|\|\| \| + +\|\| \|# +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \| Sbjct 340 DFMREFTRLEICNLTPDALKSRTIRKWNTT#LYEGTWRRGSTAGGCRNYPATFWVNPQFKI 399
synthetic construct	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|+\| ++\|\|\|\|\|\|\|\|\|\| \| + +\|\| \|# +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \| Sbjct 340 DFMREFTRLEICNLTPDALKSRTIRKWNTT#LYEGTWRRGSTAGGCRNYPATFWVNPQFKI 399
Bos taurus	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|+\| ++\|\|\|\|\|\|\|\|\|\| \| ++ \| \|# +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \| Sbjct 340 DFMREFTRLEICNLTPDALKSQRFRNWNTT#LYEGTWRRGSTAGGCRNYPATFWVNPQFKI 399
Sus scrofa	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\| ++\|\|\|\|\|\|\|\|\|\| \| + \| \|# +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \| Sbjct 340 DFLREFTRLEICNLTPDALKSQRVRNWNTT#LYEGTWRRGSTAGGCRNYPATFWVNPQFKI 399
Coturnix coturnix	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|+\| +\|\|\|\|\|\|\|\|\|\|\| \|\| \| +\| # +\| \|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|+\|\|\|+ \| Sbjct 333 DFMREFSRLEICNLTPDALTKDELSRWHTQ#VFEGTWRRGSTAGGCRNNPATFWINPQFKI 392
Danio rerio	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\|\| ++\|\|\|\|\|\|\|\| \| \| \|\|\| # +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| \|\|\|+ + Sbjct 335 DFLREFTRLEICNLTADALQASQVKKWSTA#NYNGEWRRGSTAGGCRNYPATFWNNPQFKV 394
Rattus norvegicus	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|+\| +++\|\|\|\|\|\|\|\|\| \| + + \| \|# +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \| Sbjct 340 DFIREFTKLEICNLTPDALKSRTLRNWNTT#FYEGTWRRGSTAGGCRNYPATFWVNPQFKI 399
Mus musculus	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQY 387 \|\|\|+ +\|\|\|\|\|\|\|\|+\|\|+\|+ + +\|\| \| # +\| \| \|\|\|\|\|\|\|\|+\|\|\| \|+\| \|\|\|+ Sbjct 5 DFLKQFSRLEICNLSPDSLSSEEIQKWNLV#LFNGRWTRGSTAGGCQNYPATYWTNPQF 62
Oncorhynchus mykiss	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQY 387 \|\| +\| ++\|\|\|\|\|\|\|\|+\|\| \|+ +\|\|++ # +\|\|\| \|\|\|\|\|\|\|\|\|\|+ +\|\|\| \|\|\|+ Sbjct 309 DFKANYDKIEICNLTPDSLTDDTKRKWEVN#MFEGNWIRGSTAGGCRNFIDTFWTNPQF 366
Drosophila melanogaster	Query 330 DFLRHYSRLEICNLTPDTLTCD----SYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNP 385 \|\|\| \|+ \|+\|\|\|\|\|+\|\|+\|\| \| \| +\|\|+++# +\| \| \| \|\|\|\|\|\|\|+ \|\|\| \|\| Sbjct 544 DFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMS#MFEGEWTSGVTAGGCRNFLETFWHNP 603 Query 386 QYLI 389 \|\|+\| Sbjct 604 QYII 607
Macaca fascicularis	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQY 387 \|\|+ \|+++\|\|\|\|\|\|\| \| \| \| + \| ++# +\| \| \|\| +\|\|\|\|\|\|+\|+\|\|\| \|\|\|\| Sbjct 403 DFIYHFTKLEICNLTADALQSDKLQTWTVS#VNEGRWVRGCSAGGCRNFPDTFWTNPQY 460
Drosophila pseudoobscura	Query 330 DFLRHYSRLEICNLTPDTLTCD----SYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNP 385 \|\|\| \|+ \|+\|\|\|\|\| \|\|+\|\| \| \| +\|\|+++# +\| \| \| \|\|\|\|\|\|\|+ \|\|\| \|\| Sbjct 550 DFLNHFDRVEICNLNPDSLTEDQQNSSRRKWEMS#MFEGEWTSGVTAGGCRNFLETFWHNP 609 Query 386 QYLI 389 \|\|+\| Sbjct 610 QYVI 613
Drosophila melanogaster	Query 330 DFLRHYSRLEICNLTPDTLTCDSY----KKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNP 385 \|\|\| \|+ \|+\|\|\|\|\|+\|\|+\|\| \| +\|\|+++# +\| \| \| \|\|\|\|\|\|\|+ +\|\|\| \|\| Sbjct 350 DFLNHFDRVEICNLSPDSLTEDQQHSGKRKWEMS#MYEGEWTPGVTAGGCRNFLDTFWHNP 409 Query 386 QYLI 389 \|\|+\| Sbjct 410 QYII 413
Oryctolagus cuniculus	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQY 387 \|\|+ \|+++\|\|\|\|\|\|\| \| \| \| + \| ++# +\| \| \|\| +\|\|\|\|\|\|+\|+\|\|\| \|\|\|\| Sbjct 335 DFVFHFTKLEICNLTADALESDKLQTWTVS#VNEGRWVRGCSAGGCRNFPDTFWTNPQY 392
Ovis aries	Query 330 DFLRHYSRLEICNLTPDTLTCDSYKKWKLT#KMDGNWRRGSTAGGCRNYPNTFWMNPQY 387 \|\|+ \|+++\|\|\|\|\|\|\| \| \| \| + \| ++# +\| \| \|\| +\|\|\|\|\|\|+\|+\|\|\| \|\|\|\| Sbjct 404 DFIYHFTKLEICNLTADALESDKLQTWTVS#VNEGRWVRGCSAGGCRNFPDTFWTNPQY 461

organism	matching
N/A	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390
Mus musculus	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390
Rattus norvegicus	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390
Homo sapiens	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 331 FLRHYSRLEICNLTPDTLTSDTYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390
Homo sapiens	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 331 FLRHYSRLEICNLTPDTLTSDTYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390
Macaca mulatta	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 331 FLRHYSRLEICNLTPDTLTSDTYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390
Bos grunniens	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 43 FLRHYSRLEICNLTPDTLTSDTYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 102
Xenopus tropicalis	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| \| \|\| \|\|\|#\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|+\|\| Sbjct 331 FLRNYSRLEICNLTPDTLASDKYSKWSLTK#MDGTWRRGSTAGGCRNFPETFWMNPQYVIK 390
Gallus gallus	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|\| \| \|#+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 331 FLRHYSRLEICNLTPDTLASDRYKKWSLLK#LDGNWRRGATAGGCRNYPNTFWTNPQYLIK 390
Stizostedion vitreum vitreum	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\| \|+ \|+ \|\| \|+\|# \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\|\|++\|+ Sbjct 332 FLRHYDRLEICNLTPDALSDDTVSKWALSK#FDGSWRRGSTAGGCRNYPDTFWTNPQFVIR 391
Tetraodon nigroviridis	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\| \| \|\|\|\| \|+# + \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|+\|\|\|++\|\| Sbjct 337 FLRQYSRLEICNLTPDALTGDEYKKWAETE#FEDTWRRGSTAGGCRNYPNSFWLNPQFVIK 396
Danio rerio	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|+\| \|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\| \| \| + \|# \|\| \|\|+\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|+ \|\| Sbjct 329 FVRQYSRLEICTLTPDTLTSDSLKHWSVCK#FDGTWRKGSTAGGCRNHPYTFWMNPQFKIK 388
Oncorhynchus mykiss	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\|\|\|\|\|+\|\|\| \|\|\|\|\|\|\| \|\| \| \| + # \|\|+\|\|+\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\|++\|+ Sbjct 329 FLRHYSRIEICTLTPDTLTDDSVKHWSVCN#YDGSWRKGSTAGGCRNHAYTFWMNPQFVIR 388
Xenopus laevis	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\| +\|\|\|\|\|\|\|\|\|\|\| \|\| \|+\|\| \| # +\|+\|\|+\|\|\|\|\|\|\|\|\|+\| \|\|\|+\|\|\|+ \|\| Sbjct 341 FLREFSRLEICNLTPDALTARRYRKWNTTV#YNGSWRKGSTAGGCRNFPATFWINPQFKIK 400
Canis familiaris	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|+\| ++\|\|\|\|\|\|\|\|\|\| \| \| +\|\| \| # +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+ Sbjct 338 FMREFTRLEICNLTPDALKSRSIRKWNTTL#YEGTWRRGSTAGGCRNYPATFWVNPQFKIR 397
Xenopus laevis	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\| +\|\|\|\|\|\|\|\|\|\|\|\|\| + \|\| \| # \|\|+\|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|+\|\|\|+ + Sbjct 334 FLRQFSRLEICNLTPDTLNKEGLSKWHTTL#YDGSWRRGSTAGGCRNNPATFWINPQFKV 392
Gallus gallus	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\| ++\|\|\|\|\|\|\|\|\|\| \| ++\|\| # \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \| Sbjct 341 FLREFTRLEICNLTPDALQSRKFRKWNTRL#YDGSWRRGSTAGGCRNYPATFWINPQFKI 399
synthetic construct	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|+\| ++\|\|\|\|\|\|\|\|\|\| \| + +\|\| \| # +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+ Sbjct 341 FMREFTRLEICNLTPDALKSRTIRKWNTTL#YEGTWRRGSTAGGCRNYPATFWVNPQFKIR 400
Pongo pygmaeus	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|+\| ++\|\|\|\|\|\|\|\|\|\| \| + +\|\| \| # +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+ Sbjct 341 FMREFTRLEICNLTPDALKSRTIRKWNTTL#YEGTWRRGSTAGGCRNYPATFWVNPQFKIR 400
Coturnix coturnix	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|+\| +\|\|\|\|\|\|\|\|\|\|\| \|\| \| +\| # +\| \|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|+\|\|\|+ \|\| Sbjct 334 FMREFSRLEICNLTPDALTKDELSRWHTQV#FEGTWRRGSTAGGCRNNPATFWINPQFKIK 393
Bos taurus	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|+\| ++\|\|\|\|\|\|\|\|\|\| \| ++ \| \| # +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+ Sbjct 341 FMREFTRLEICNLTPDALKSQRFRNWNTTL#YEGTWRRGSTAGGCRNYPATFWVNPQFKIR 400
Sus scrofa	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|\|\| ++\|\|\|\|\|\|\|\|\|\| \| + \| \| # +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+ Sbjct 341 FLREFTRLEICNLTPDALKSQRVRNWNTTL#YEGTWRRGSTAGGCRNYPATFWVNPQFKIR 400
Rattus norvegicus	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|+\| +++\|\|\|\|\|\|\|\|\| \| + + \| \| # +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+ Sbjct 341 FIREFTKLEICNLTPDALKSRTLRNWNTTF#YEGTWRRGSTAGGCRNYPATFWVNPQFKIR 400
Danio rerio	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLI 389 \|\|\| ++\|\|\|\|\|\|\|\| \| \| \|\|\| # +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| \|\|\|+ + Sbjct 336 FLREFTRLEICNLTADALQASQVKKWSTAN#YNGEWRRGSTAGGCRNYPATFWNNPQFKV 394
Oncorhynchus mykiss	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQY 387 \| +\| ++\|\|\|\|\|\|\|\|+\|\| \|+ +\|\|++ # +\|\|\| \|\|\|\|\|\|\|\|\|\|+ +\|\|\| \|\|\|+ Sbjct 310 FKANYDKIEICNLTPDSLTDDTKRKWEVNM#FEGNWIRGSTAGGCRNFIDTFWTNPQF 366
Mus musculus	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQY 387 \|\|+ +\|\|\|\|\|\|\|\|+\|\|+\|+ + +\|\| \| # +\| \| \|\|\|\|\|\|\|\|+\|\|\| \|+\| \|\|\|+ Sbjct 6 FLKQFSRLEICNLSPDSLSSEEIQKWNLVL#FNGRWTRGSTAGGCQNYPATYWTNPQF 62
Macaca fascicularis	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|+ \|+++\|\|\|\|\|\|\| \| \| \| + \| ++ # +\| \| \|\| +\|\|\|\|\|\|+\|+\|\|\| \|\|\|\| +\| Sbjct 404 FIYHFTKLEICNLTADALQSDKLQTWTVSV#NEGRWVRGCSAGGCRNFPDTFWTNPQYRLK 463
Drosophila melanogaster	Query 331 FLRHYSRLEICNLTPDTLTCD----SYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQ 386 \|\| \|+ \|+\|\|\|\|\|+\|\|+\|\| \| \| +\|\|+++ # +\| \| \| \|\|\|\|\|\|\|+ \|\|\| \|\|\| Sbjct 545 FLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSM#FEGEWTSGVTAGGCRNFLETFWHNPQ 604 Query 387 YLI 389 \|+\| Sbjct 605 YII 607
Drosophila melanogaster	Query 331 FLRHYSRLEICNLTPDTLTCD----SYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQ 386 \|\| \|+ \|+\|\|\|\|\|+\|\|+\|\| \| \| +\|\|+++ # +\| \| \| \|\|\|\|\|\|\|+ \|\|\| \|\|\| Sbjct 545 FLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSM#FEGEWTSGVTAGGCRNFLETFWHNPQ 604 Query 387 YLI 389 \|+\| Sbjct 605 YII 607
Drosophila pseudoobscura	Query 331 FLRHYSRLEICNLTPDTLTCD----SYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQ 386 \|\| \|+ \|+\|\|\|\|\| \|\|+\|\| \| \| +\|\|+++ # +\| \| \| \|\|\|\|\|\|\|+ \|\|\| \|\|\| Sbjct 551 FLNHFDRVEICNLNPDSLTEDQQNSSRRKWEMSM#FEGEWTSGVTAGGCRNFLETFWHNPQ 610 Query 387 YLI 389 \|+\| Sbjct 611 YVI 613
Oryctolagus cuniculus	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|+ \|+++\|\|\|\|\|\|\| \| \| \| + \| ++ # +\| \| \|\| +\|\|\|\|\|\|+\|+\|\|\| \|\|\|\| +\| Sbjct 336 FVFHFTKLEICNLTADALESDKLQTWTVSV#NEGRWVRGCSAGGCRNFPDTFWTNPQYRLK 395
Ovis aries	Query 331 FLRHYSRLEICNLTPDTLTCDSYKKWKLTK#MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK 390 \|+ \|+++\|\|\|\|\|\|\| \| \| \| + \| ++ # +\| \| \|\| +\|\|\|\|\|\|+\|+\|\|\| \|\|\|\| +\| Sbjct 405 FIYHFTKLEICNLTADALESDKLQTWTVSV#NEGRWVRGCSAGGCRNFPDTFWTNPQYRLK 464

organism	matching
N/A	Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411
Rattus norvegicus	Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411
Mus musculus	Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDEEDGERGCTFLVGL 411
Xenopus tropicalis	Query 353 YKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 \| \|\| \|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|#\|\|\|\|\|\|+\|\|\|\|\| \|+\| +\| \| \|\|\|\|+\|\|\| Sbjct 353 YSKWSLTKMDGTWRRGSTAGGCRNFPETF#WMNPQYVIKLEEADDDPDDNEEGCTFIVGL 411
Homo sapiens	Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\| Sbjct 352 TYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDEEDGESGCTFLVGL 411
Homo sapiens	Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\| Sbjct 352 TYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDEEDGESGCTFLVGL 411
Macaca mulatta	Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\| Sbjct 352 TYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDEEDGESGCTFLVGL 411
Stizostedion vitreum vitreum	Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 + \|\| \|+\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|#\| \|\|\|++\|+\|\|\|\|\|+\| \|\|\| \|\|+\|+\|\|\| Sbjct 353 TVSKWALSKFDGSWRRGSTAGGCRNYPDTF#WTNPQFVIRLEEEDDDPADGELGCSFVVGL 412
Bos grunniens	Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\| Sbjct 64 TYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDQEDGESGCTFLVGL 123
Tetraodon nigroviridis	Query 353 YKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 \|\|\|\| \|+ + \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|#\|+\|\|\|++\|\|\|\|\| \|+\| + + \|\|\|\|+\|\|\| Sbjct 359 YKKWAETEFEDTWRRGSTAGGCRNYPNSF#WLNPQFVIKLEEVDDDPGEDQEGCTFIVGL 417
Gallus gallus	Query 353 YKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 \|\|\|\| \| \|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|#\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| +\| \| \|\|\|\|\|+\|\| Sbjct 353 YKKWSLLKLDGNWRRGATAGGCRNYPNTF#WTNPQYLIKLEEEDEDPDDPEGGCTFLIGL 411
Xenopus laevis	Query 353 YKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDG-ERGCTFLVGL 411 \|+\|\| \| +\|+\|\|+\|\|\|\|\|\|\|\|\|+\| \|\|#\|+\|\|\|+ \|\|\|\|\|\|\| \|\|+ \| \| \|\|\|\|\|+ \| Sbjct 363 YRKWNTTVYNGSWRKGSTAGGCRNFPATF#WINPQFKIKLEEEDGDDDYGSETGCTFLLAL 422
Danio rerio	Query 355 KWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 +\| ++ +\|\|\|\| \|\|\|\|\|\|\|\|\| \| \|\|# \|\|\|++\|\|\|\|\| \|+\| \|\| \|\|\|\|\|\|\|\| Sbjct 355 RWSYSQFEGNWRVGSTAGGCRNNPATF#CSNPQFMIKLEEIDDDPHDGVNGCTFLVGL 411
Canis familiaris	Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDED---GERGCTFL 408 \| +\|\| \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|#\|+\|\|\|+ \|+\|\|\| \|+ \|+\| \| \|\|+\|+ Sbjct 359 SIRKWNTTLYEGTWRRGSTAGGCRNYPATF#WVNPQFKIRLEETDDVDDDYGGRESGCSFV 418 Query 409 VGL 411 + \| Sbjct 419 LAL 421
Pongo pygmaeus	Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGER--GCTFLV 409 + +\|\| \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|#\|+\|\|\|+ \|+\|+\| \|+ \|+ \|+\| \|\|+\|++ Sbjct 362 TIRKWNTTLYEGTWRRGSTAGGCRNYPATF#WVNPQFKIRLDETDDPDDYGDRESGCSFVL 421 Query 410 GL 411 \| Sbjct 422 AL 423
synthetic construct	Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGER--GCTFLV 409 + +\|\| \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|#\|+\|\|\|+ \|+\|+\| \|+ \|+ \|+\| \|\|+\|++ Sbjct 362 TIRKWNTTLYEGTWRRGSTAGGCRNYPATF#WVNPQFKIRLDETDDPDDYGDRESGCSFVL 421 Query 410 GL 411 \| Sbjct 422 AL 423
Rattus norvegicus	Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEE-EDEDDEDG-ERGCTFLV 409 + + \| \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|#\|+\|\|\|+ \|+\|\|\| +\| \|\| \| \| \|\|+\|\|+ Sbjct 362 TLRNWNTTFYEGTWRRGSTAGGCRNYPATF#WVNPQFKIRLEEVDDADDYDSRESGCSFLL 421 Query 410 GL 411 \| Sbjct 422 AL 423
Sus scrofa	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEED--EDDEDG-ERGCTFLVG 410 + \| \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|#\|+\|\|\|+ \|+\|\|\| \| \|\|\| \| \| \|\|+\|++ Sbjct 364 RNWNTTLYEGTWRRGSTAGGCRNYPATF#WVNPQFKIRLEETDDPEDDYGGRESGCSFVLA 423 Query 411 L 411 \| Query 411 L 411
Oncorhynchus mykiss	Query 352 SYKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 \| \| \| + \|\|+\|\|+\|\|\|\|\|\|\|\|\|+ \|\|#\|\|\|\|\|++\|+\|+\|\|\|+\| +\| \| \|\|+\|+\|\|\| Sbjct 350 SVKHWSVCNYDGSWRKGSTAGGCRNHAYTF#WMNPQFVIRLDEEDDDPDDNEVGCSFVVGL 409
Bos taurus	Query 353 YKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDG----ERGCTFL 408 ++ \| \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|#\|+\|\|\|+ \|+\|\|\| \|+ \| \| \| \|\|+\|\| Sbjct 363 FRNWNTTLYEGTWRRGSTAGGCRNYPATF#WVNPQFKIRLEETDDPDPDDYGGRESGCSFL 422 Query 409 VGL 411 + \| Sbjct 423 LAL 425
Gallus gallus	Query 353 YKKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDG---ERGCTFLV 409 ++\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|#\|+\|\|\|+ \| \|\|\| \|+\| +\| \| \|\|+\|\|+ Sbjct 363 FRKWNTRLYDGSWRRGSTAGGCRNYPATF#WINPQFKICLEEVDDDGDDFGGREPGCSFLL 422 Query 410 GL 411 \| Sbjct 423 AL 424
Schistosoma japonicum	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 ++\|\|+\| +\| \| \| \|\|\|\|\|\| \|+\|\|\|\|#+\|\|\|\|+ +++ + \|\| \|+\|\| \| +\|\|\| Sbjct 61 RRWKITCEEGEWLRNSTAGGCTNFPNTF#YMNPQFHVQIVDPDESDDDGTG--TLVVGL 116
Danio rerio	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 \|\|\| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|#\| \|\|\|+ + \|+ \| \|+ \|+\|\|\| \| Sbjct 359 KKWSTANYNGEWRRGSTAGGCRNYPATF#WNNPQFKVALKHP---DSPGQSECSFLVAL 413
Drosophila melanogaster	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 +\|\|+++ +\| \| \| \|\|\|\|\|\|\|+ +\|\|#\| \|\|\|\|+\| \| + \|\|+\|\|+\|+ \|\| +\| \| Sbjct 378 RKWEMSMYEGEWTPGVTAGGCRNFLDTF#WHNPQYIITLVDPDEEDEEGQ--CTVIVAL 433
Drosophila melanogaster	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 +\|\|+++ +\| \| \| \|\|\|\|\|\|\|+ +\|\|#\| \|\|\|\|+\| \| + \|\|+\|\|+\|+ \|\| +\| \| Sbjct 401 RKWEMSMYEGEWTPGVTAGGCRNFLDTF#WHNPQYIITLVDPDEEDEEGQ--CTVIVAL 456
Xenopus laevis	Query 355 KWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 \|\| \| \|\|+\|\|\|\|\|\|\|\|\|\|\|\| \| \|\|#\|+\|\|\|+ + \| \|\|\|+\| +\| \| \|+\|\|\| \| Sbjct 358 KWHTTLYDGSWRRGSTAGGCRNNPATF#WINPQFKVTLLEEDDDPDDNELACSFLVAL 414
Coturnix coturnix	Query 355 KWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 +\| +\| \|\|\|\|\|\|\|\|\|\|\|\| \| \|\|#\|+\|\|\|+ \|\|\| \|\|\|+\| \| \| \|+\|\|\| \| Sbjct 358 RWHTQVFEGTWRRGSTAGGCRNNPATF#WINPQFKIKLLEEDDDPGDDEVACSFLVAL 414
Oncorhynchus mykiss	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 +\|\|++ +\|\|\| \|\|\|\|\|\|\|\|\|\|+ +\|\|#\| \|\|\|+ + \| \|++++ \|+ ++ \| Sbjct 333 RKWEVNMFEGNWIRGSTAGGCRNFIDTF#WTNPQFKLTLLHADDNNDK----CSVVIAL 386
Oryctolagus cuniculus	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 + \| ++ +\| \| \|\| +\|\|\|\|\|\|+\|+\|\|#\| \|\|\|\| +\|\| \|\|\|+\| \|\| \| \|+\|\|\| \| Sbjct 359 QTWTVSVNEGRWVRGCSAGGCRNFPDTF#WTNPQYRLKLLEEDDDPEDSEVICSFLVAL 416
Macaca fascicularis	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTF#WMNPQYLIKLEEEDEDDEDGERGCTFLVGL 411 + \| ++ +\| \| \|\| +\|\|\|\|\|\|+\|+\|\|#\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|\|\| \| Sbjct 427 QTWTVSVNEGRWVRGCSAGGCRNFPDTF#WTNPQYRLKLLEEDDDPDDSEVICSFLVAL 484

organism	matching
N/A	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413
Rattus norvegicus	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413
Mus musculus	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDEEDGERGCTFLVGLIQ 413
Homo sapiens	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDEEDGESGCTFLVGLIQ 413
Homo sapiens	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDEEDGESGCTFLVGLIQ 413
Macaca mulatta	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDEEDGESGCTFLVGLIQ 413
Bos grunniens	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 66 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDQEDGESGCTFLVGLIQ 125
Xenopus tropicalis	Query 355 KWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\| \|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|#\|\|\|\|+\|\|\|\|\| \|+\| +\| \| \|\|\|\|+\|\|\|\|\| Sbjct 355 KWSLTKMDGTWRRGSTAGGCRNFPETFWM#NPQYVIKLEEADDDPDDNEEGCTFIVGLIQ 413
Stizostedion vitreum vitreum	Query 355 KWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\| \|+\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| #\|\|\|++\|+\|\|\|\|\|+\| \|\|\| \|\|+\|+\|\|\|\|\| Sbjct 356 KWALSKFDGSWRRGSTAGGCRNYPDTFWT#NPQFVIRLEEEDDDPADGELGCSFVVGLIQ 414
Tetraodon nigroviridis	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\|\| \|+ + \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|+#\|\|\|++\|\|\|\|\| \|+\| + + \|\|\|\|+\|\|\|+\| Sbjct 360 KKWAETEFEDTWRRGSTAGGCRNYPNSFWL#NPQFVIKLEEVDDDPGEDQEGCTFIVGLMQ 419
Gallus gallus	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\|\| \| \|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| #\|\|\|\|\|\|\|\|\|\|\|\|\|\| +\| \| \|\|\|\|\|+\|\|\|\| Sbjct 354 KKWSLLKLDGNWRRGATAGGCRNYPNTFWT#NPQYLIKLEEEDEDPDDPEGGCTFLIGLIQ 413
Xenopus laevis	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDG-ERGCTFLVGLI 412 +\|\| \| +\|+\|\|+\|\|\|\|\|\|\|\|\|+\| \|\|\|+#\|\|\|+ \|\|\|\|\|\|\| \|\|+ \| \| \|\|\|\|\|+ \|+ Sbjct 364 RKWNTTVYNGSWRKGSTAGGCRNFPATFWI#NPQFKIKLEEEDGDDDYGSETGCTFLLALM 423 Query 413 Q 413 Query 413 Q 413
Danio rerio	Query 355 KWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 +\| ++ +\|\|\|\| \|\|\|\|\|\|\|\|\| \| \|\| #\|\|\|++\|\|\|\|\| \|+\| \|\| \|\|\|\|\|\|\|\|+\| Sbjct 355 RWSYSQFEGNWRVGSTAGGCRNNPATFCS#NPQFMIKLEEIDDDPHDGVNGCTFLVGLMQ 413
Canis familiaris	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDED---GERGCTFLVG 410 +\|\| \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+#\|\|\|+ \|+\|\|\| \|+ \|+\| \| \|\|+\|++ Sbjct 361 RKWNTTLYEGTWRRGSTAGGCRNYPATFWV#NPQFKIRLEETDDVDDDYGGRESGCSFVLA 420 Query 411 LIQ 413 \|+\| Sbjct 421 LMQ 423
Pongo pygmaeus	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGER--GCTFLVGL 411 +\|\| \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+#\|\|\|+ \|+\|+\| \|+ \|+ \|+\| \|\|+\|++ \| Sbjct 364 RKWNTTLYEGTWRRGSTAGGCRNYPATFWV#NPQFKIRLDETDDPDDYGDRESGCSFVLAL 423 Query 412 IQ 413 +\| Sbjct 424 MQ 425
synthetic construct	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGER--GCTFLVGL 411 +\|\| \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+#\|\|\|+ \|+\|+\| \|+ \|+ \|+\| \|\|+\|++ \| Sbjct 364 RKWNTTLYEGTWRRGSTAGGCRNYPATFWV#NPQFKIRLDETDDPDDYGDRESGCSFVLAL 423 Query 412 IQ 413 +\| Sbjct 424 MQ 425
Rattus norvegicus	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEE-EDEDDEDG-ERGCTFLVGL 411 + \| \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+#\|\|\|+ \|+\|\|\| +\| \|\| \| \| \|\|+\|\|+ \| Sbjct 364 RNWNTTFYEGTWRRGSTAGGCRNYPATFWV#NPQFKIRLEEVDDADDYDSRESGCSFLLAL 423 Query 412 IQ 413 +\| Sbjct 424 MQ 425
Sus scrofa	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEED--EDDEDG-ERGCTFLVG 410 + \| \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+#\|\|\|+ \|+\|\|\| \| \|\|\| \| \| \|\|+\|++ Sbjct 364 RNWNTTLYEGTWRRGSTAGGCRNYPATFWV#NPQFKIRLEETDDPEDDYGGRESGCSFVLA 423 Query 411 LIQ 413 \|+\| Sbjct 424 LMQ 426
Oncorhynchus mykiss	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \| \| + \|\|+\|\|+\|\|\|\|\|\|\|\|\|+ \|\|\|\|#\|\|\|++\|+\|+\|\|\|+\| +\| \| \|\|+\|+\|\|\|\|\| Sbjct 352 KHWSVCNYDGSWRKGSTAGGCRNHAYTFWM#NPQFVIRLDEEDDDPDDNEVGCSFVVGLIQ 411
Bos taurus	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDG----ERGCTFLV 409 + \| \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+#\|\|\|+ \|+\|\|\| \|+ \| \| \| \|\|+\|\|+ Sbjct 364 RNWNTTLYEGTWRRGSTAGGCRNYPATFWV#NPQFKIRLEETDDPDPDDYGGRESGCSFLL 423 Query 410 GLIQ 413 \|+\| Sbjct 424 ALMQ 427
Danio rerio	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\|\| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| #\|\|\|+ + \|+ \| \|+ \|+\|\|\| \|+\| Sbjct 359 KKWSTANYNGEWRRGSTAGGCRNYPATFWN#NPQFKVALKHP---DSPGQSECSFLVALMQ 415
Schistosoma japonicum	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 ++\|\|+\| +\| \| \| \|\|\|\|\|\| \|+\|\|\|\|+\|#\|\|\|+ +++ + \|\| \|+\|\| \| +\|\|\|+\| Sbjct 61 RRWKITCEEGEWLRNSTAGGCTNFPNTFYM#NPQFHVQIVDPDESDDDGTG--TLVVGLMQ 118
Gallus gallus	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDG---ERGCTFLVG 410 +\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+#\|\|\|+ \| \|\|\| \|+\| +\| \| \|\|+\|\|+ Sbjct 364 RKWNTRLYDGSWRRGSTAGGCRNYPATFWI#NPQFKICLEEVDDDGDDFGGREPGCSFLLA 423 Query 411 LIQ 413 \|+\| Sbjct 424 LMQ 426
Drosophila melanogaster	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 +\|\|+++ +\| \| \| \|\|\|\|\|\|\|+ +\|\|\| #\|\|\|\|+\| \| + \|\|+\|\|+\|+ \|\| +\| \|+\| Sbjct 378 RKWEMSMYEGEWTPGVTAGGCRNFLDTFWH#NPQYIITLVDPDEEDEEGQ--CTVIVALMQ 435
Drosophila melanogaster	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 +\|\|+++ +\| \| \| \|\|\|\|\|\|\|+ +\|\|\| #\|\|\|\|+\| \| + \|\|+\|\|+\|+ \|\| +\| \|+\| Sbjct 401 RKWEMSMYEGEWTPGVTAGGCRNFLDTFWH#NPQYIITLVDPDEEDEEGQ--CTVIVALMQ 458
Xenopus laevis	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\| \| \|\|+\|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|+#\|\|\|+ + \| \|\|\|+\| +\| \| \|+\|\|\| \|+\| Sbjct 357 SKWHTTLYDGSWRRGSTAGGCRNNPATFWI#NPQFKVTLLEEDDDPDDNELACSFLVALMQ 416
Coturnix coturnix	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 +\| +\| \|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|+#\|\|\|+ \|\|\| \|\|\|+\| \| \| \|+\|\|\| \|+\| Sbjct 357 SRWHTQVFEGTWRRGSTAGGCRNNPATFWI#NPQFKIKLLEEDDDPGDDEVACSFLVALMQ 416
Macaca fascicularis	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 + \| ++ +\| \| \|\| +\|\|\|\|\|\|+\|+\|\|\| #\|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|\|\| \|+\| Sbjct 427 QTWTVSVNEGRWVRGCSAGGCRNFPDTFWT#NPQYRLKLLEEDDDPDDSEVICSFLVALMQ 486
Oncorhynchus mykiss	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 +\|\|++ +\|\|\| \|\|\|\|\|\|\|\|\|\|+ +\|\|\| #\|\|\|+ + \| \|++++ \|+ ++ \|+\| Sbjct 333 RKWEVNMFEGNWIRGSTAGGCRNFIDTFWT#NPQFKLTLLHADDNNDK----CSVVIALMQ 388
Oryctolagus cuniculus	Query 354 KKWKLTKMDGNWRRGSTAGGCRNYPNTFWM#NPQYLIKLEEEDEDDEDGERGCTFLVGLIQ 413 + \| ++ +\| \| \|\| +\|\|\|\|\|\|+\|+\|\|\| #\|\|\|\| +\|\| \|\|\|+\| \|\| \| \|+\|\|\| \|+\| Sbjct 359 QTWTVSVNEGRWVRGCSAGGCRNFPDTFWT#NPQYRLKLLEEDDDPEDSEVICSFLVALMQ 418

organism	matching
N/A	Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417
Rattus norvegicus	Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417
Mus musculus	Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDEEDGERGCTFLVGLIQKHRR 417
Homo sapiens	Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDEEDGESGCTFLVGLIQKHRR 417
Homo sapiens	Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDEEDGESGCTFLVGLIQKHRR 417
Macaca mulatta	Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDEEDGESGCTFLVGLIQKHRR 417
Bos grunniens	Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQ 413 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 70 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDQEDGESGCTFLVGLIQ 125
Xenopus tropicalis	Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 \|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|#+\|\|\|\|\| \|+\| +\| \| \|\|\|\|+\|\|\|\|\|\|+\|\| Sbjct 358 LTKMDGTWRRGSTAGGCRNFPETFWMNPQY#VIKLEEADDDPDDNEEGCTFIVGLIQKNRR 417
Stizostedion vitreum vitreum	Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 \|+\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\|\|+#+\|+\|\|\|\|\|+\| \|\|\| \|\|+\|+\|\|\|\|\|\|+\|\| Sbjct 359 LSKFDGSWRRGSTAGGCRNYPDTFWTNPQF#VIRLEEEDDDPADGELGCSFVVGLIQKNRR 418
Gallus gallus	Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 \| \|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|#\|\|\|\|\|\|\|\|\|\| +\| \| \|\|\|\|\|+\|\|\|\|\|\|\|\| Sbjct 358 LLKLDGNWRRGATAGGCRNYPNTFWTNPQY#LIKLEEEDEDPDDPEGGCTFLIGLIQKHRR 417
Tetraodon nigroviridis	Query 359 TKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 \|+ + \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|+\|\|\|+#+\|\|\|\|\| \|+\| + + \|\|\|\|+\|\|\|+\|\|+\|\| Sbjct 365 TEFEDTWRRGSTAGGCRNYPNSFWLNPQF#VIKLEEVDDDPGEDQEGCTFIVGLMQKNRR 423
Xenopus laevis	Query 359 TKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDG-ERGCTFLVGLIQKHRR 417 \| +\|+\|\|+\|\|\|\|\|\|\|\|\|+\| \|\|\|+\|\|\|+# \|\|\|\|\|\|\| \|\|+ \| \| \|\|\|\|\|+ \|+\|\|++\| Sbjct 369 TVYNGSWRKGSTAGGCRNFPATFWINPQF#KIKLEEEDGDDDYGSETGCTFLLALMQKNKR 428
Danio rerio	Query 359 TKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 ++ +\|\|\|\| \|\|\|\|\|\|\|\|\| \| \|\| \|\|\|+#+\|\|\|\|\| \|+\| \|\| \|\|\|\|\|\|\|\|+\|\| \| Sbjct 359 SQFEGNWRVGSTAGGCRNNPATFCSNPQF#MIKLEEIDDDPHDGVNGCTFLVGLMQKDGR 417
Rattus norvegicus	Query 359 TKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEE-EDEDDEDG-ERGCTFLVGLIQKH 415 \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+# \|+\|\|\| +\| \|\| \| \| \|\|+\|\|+ \|+\|\|\| Sbjct 369 TFYEGTWRRGSTAGGCRNYPATFWVNPQF#KIRLEEVDDADDYDSRESGCSFLLALMQKH 427
Pongo pygmaeus	Query 359 TKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGER--GCTFLVGLIQKH 415 \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+# \|+\|+\| \|+ \|+ \|+\| \|\|+\|++ \|+\|\|\| Sbjct 369 TLYEGTWRRGSTAGGCRNYPATFWVNPQF#KIRLDETDDPDDYGDRESGCSFVLALMQKH 427
synthetic construct	Query 359 TKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGER--GCTFLVGLIQKH 415 \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+# \|+\|+\| \|+ \|+ \|+\| \|\|+\|++ \|+\|\|\| Sbjct 369 TLYEGTWRRGSTAGGCRNYPATFWVNPQF#KIRLDETDDPDDYGDRESGCSFVLALMQKH 427
Canis familiaris	Query 359 TKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDG---ERGCTFLVGLIQKH 415 \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+# \|+\|\|\| \|+ \|+\| \| \|\|+\|++ \|+\|\|\| Sbjct 366 TLYEGTWRRGSTAGGCRNYPATFWVNPQF#KIRLEETDDVDDDYGGRESGCSFVLALMQKH 425
Sus scrofa	Query 359 TKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEED--EDDEDG-ERGCTFLVGLIQKH 415 \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+# \|+\|\|\| \| \|\|\| \| \| \|\|+\|++ \|+\|\|\| Sbjct 369 TLYEGTWRRGSTAGGCRNYPATFWVNPQF#KIRLEETDDPEDDYGGRESGCSFVLALMQKH 428
Gallus gallus	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDG---ERGCTFLVGLIQKHRR 417 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+# \| \|\|\| \|+\| +\| \| \|\|+\|\|+ \|+\|\|\|\|\| Sbjct 372 DGSWRRGSTAGGCRNYPATFWINPQF#KICLEEVDDDGDDFGGREPGCSFLLALMQKHRR 430
Oncorhynchus mykiss	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 \|\|+\|\|+\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\|+#+\|+\|+\|\|\|+\| +\| \| \|\|+\|+\|\|\|\|\|\|+\|\| Sbjct 360 DGSWRKGSTAGGCRNHAYTFWMNPQF#VIRLDEEDDDPDDNEVGCSFVVGLIQKNRR 415
Bos taurus	Query 359 TKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDG----ERGCTFLVGLIQK 414 \| +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+# \|+\|\|\| \|+ \| \| \| \|\|+\|\|+ \|+\|\| Sbjct 369 TLYEGTWRRGSTAGGCRNYPATFWVNPQF#KIRLEETDDPDPDDYGGRESGCSFLLALMQK 428 Query 415 H 415 \| Query 415 H 415
Coturnix coturnix	Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 +\| \|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|+\|\|\|+# \|\|\| \|\|\|+\| \| \| \|+\|\|\| \|+\|\|\|\|\| Sbjct 364 FEGTWRRGSTAGGCRNNPATFWINPQF#KIKLLEEDDDPGDDEVACSFLVALMQKHRR 420
Danio rerio	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| \|\|\|+# + \|+ \| \|+ \|+\|\|\| \|+\|\| \|\| Sbjct 367 NGEWRRGSTAGGCRNYPATFWNNPQF#KVALKHP---DSPGQSECSFLVALMQKDRR 419
Drosophila melanogaster	Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 ++ +\| \| \| \|\|\|\|\|\|\|+ +\|\|\| \|\|\|\|#+\| \| + \|\|+\|\|+\|+ \|\| +\| \|+\|\|+\|\| Sbjct 382 MSMYEGEWTPGVTAGGCRNFLDTFWHNPQY#IITLVDPDEEDEEGQ--CTVIVALMQKNRR 439
Drosophila melanogaster	Query 358 LTKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 ++ +\| \| \| \|\|\|\|\|\|\|+ +\|\|\| \|\|\|\|#+\| \| + \|\|+\|\|+\|+ \|\| +\| \|+\|\|+\|\| Sbjct 405 MSMYEGEWTPGVTAGGCRNFLDTFWHNPQY#IITLVDPDEEDEEGQ--CTVIVALMQKNRR 462
Xenopus laevis	Query 359 TKMDGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 \| \|\|+\|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|+\|\|\|+# + \| \|\|\|+\| +\| \| \|+\|\|\| \|+\|\|+\|\| Sbjct 362 TLYDGSWRRGSTAGGCRNNPATFWINPQF#KVTLLEEDDDPDDNELACSFLVALMQKNRR 420
Schistosoma japonicum	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 +\| \|+\| +\|\|\|\|\|\|+ +\|\|\|+\|\|\|\|#++ +\|+ \|\|+\|\| \| \| +\|\|\|+\|\|+\|\| Sbjct 16 EGCWKRRVSAGGCRNFLDTFWINPQY#VVSVEDPDENDE--ENKGTLIVGLMQKNRR 69
Macaca fascicularis	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 +\| \| \|\| +\|\|\|\|\|\|+\|+\|\|\| \|\|\|\|# +\|\| \|\|\|+\| +\| \| \|+\|\|\| \|+\|\|+\|\| Sbjct 435 EGRWVRGCSAGGCRNFPDTFWTNPQY#RLKLLEEDDDPDDSEVICSFLVALMQKNRR 490
Oryctolagus cuniculus	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQY#LIKLEEEDEDDEDGERGCTFLVGLIQKHRR 417 +\| \| \|\| +\|\|\|\|\|\|+\|+\|\|\| \|\|\|\|# +\|\| \|\|\|+\| \|\| \| \|+\|\|\| \|+\|\|+\|\| Sbjct 367 EGRWVRGCSAGGCRNFPDTFWTNPQY#RLKLLEEDDDPEDSEVICSFLVALMQKNRR 422

organism	matching
N/A	Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420
Rattus norvegicus	Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420
Mus musculus	Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDEEDGERGCTFLVGLIQKHRRRQR 420
Homo sapiens	Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDEEDGESGCTFLVGLIQKHRRRQR 420
Macaca mulatta	Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDEEDGESGCTFLVGLIQKHRRRQR 420
Homo sapiens	Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDEEDGESGCTFLVGLIQKHRRRQR 420
Xenopus tropicalis	Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 \|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|+\|\|#\|\|\| \|+\| +\| \| \|\|\|\|+\|\|\|\|\|\|+\|\|+\|\| Sbjct 361 MDGTWRRGSTAGGCRNFPETFWMNPQYVIK#LEEADDDPDDNEEGCTFIVGLIQKNRRKQR 420
Bos grunniens	Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQ 413 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 73 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDQEDGESGCTFLVGLIQ 125
Stizostedion vitreum vitreum	Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\|\|++\|+#\|\|\|\|\|+\| \|\|\| \|\|+\|+\|\|\|\|\|\|+\|\|\| \| Sbjct 362 FDGSWRRGSTAGGCRNYPDTFWTNPQFVIR#LEEEDDDPADGELGCSFVVGLIQKNRRRAR 421
Gallus gallus	Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 +\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|#\|\|\|\|\|\|\| +\| \| \|\|\|\|\|+\|\|\|\|\|\|\|\|+\|\| Sbjct 361 LDGNWRRGATAGGCRNYPNTFWTNPQYLIK#LEEEDEDPDDPEGGCTFLIGLIQKHRRKQR 420
Tetraodon nigroviridis	Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 + \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|+\|\|\|++\|\|#\|\|\| \|+\| + + \|\|\|\|+\|\|\|+\|\|+\|\|\| \| Sbjct 367 FEDTWRRGSTAGGCRNYPNSFWLNPQFVIK#LEEVDDDPGEDQEGCTFIVGLMQKNRRRLR 426
Xenopus laevis	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDG-ERGCTFLVGLIQKHRRRQR 420 +\|+\|\|+\|\|\|\|\|\|\|\|\|+\| \|\|\|+\|\|\|+ \|\|#\|\|\|\|\| \|\|+ \| \| \|\|\|\|\|+ \|+\|\|++\|++\| Sbjct 372 NGSWRKGSTAGGCRNFPATFWINPQFKIK#LEEEDGDDDYGSETGCTFLLALMQKNKRKER 431
Danio rerio	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 +\|\|\|\| \|\|\|\|\|\|\|\|\| \| \|\| \|\|\|++\|\|#\|\|\| \|+\| \|\| \|\|\|\|\|\|\|\|+\|\| \|+ + Sbjct 362 EGNWRVGSTAGGCRNNPATFCSNPQFMIK#LEEIDDDPHDGVNGCTFLVGLMQKDGRKDK 420
Canis familiaris	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDG---ERGCTFLVGLIQKH 415 +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+#\|\|\| \|+ \|+\| \| \|\|+\|++ \|+\|\|\| Sbjct 369 EGTWRRGSTAGGCRNYPATFWVNPQFKIR#LEETDDVDDDYGGRESGCSFVLALMQKH 425
Gallus gallus	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDG---ERGCTFLVGLIQKHRRR 418 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \| #\|\|\| \|+\| +\| \| \|\|+\|\|+ \|+\|\|\|\|\|\| Sbjct 372 DGSWRRGSTAGGCRNYPATFWINPQFKIC#LEEVDDDGDDFGGREPGCSFLLALMQKHRRR 431 Query 419 QR 420 +\| Sbjct 432 ER 433
Rattus norvegicus	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEE-EDEDDEDG-ERGCTFLVGLIQKH 415 +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+#\|\|\| +\| \|\| \| \| \|\|+\|\|+ \|+\|\|\| Sbjct 372 EGTWRRGSTAGGCRNYPATFWVNPQFKIR#LEEVDDADDYDSRESGCSFLLALMQKH 427
Pongo pygmaeus	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGER--GCTFLVGLIQKH 415 +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+#\|+\| \|+ \|+ \|+\| \|\|+\|++ \|+\|\|\| Sbjct 372 EGTWRRGSTAGGCRNYPATFWVNPQFKIR#LDETDDPDDYGDRESGCSFVLALMQKH 427
synthetic construct	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGER--GCTFLVGLIQKH 415 +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+#\|+\| \|+ \|+ \|+\| \|\|+\|++ \|+\|\|\| Sbjct 372 EGTWRRGSTAGGCRNYPATFWVNPQFKIR#LDETDDPDDYGDRESGCSFVLALMQKH 427
Sus scrofa	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEED--EDDEDG-ERGCTFLVGLIQKH 415 +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+#\|\|\| \| \|\|\| \| \| \|\|+\|++ \|+\|\|\| Sbjct 372 EGTWRRGSTAGGCRNYPATFWVNPQFKIR#LEETDDPEDDYGGRESGCSFVLALMQKH 428
Oncorhynchus mykiss	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 \|\|+\|\|+\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\|++\|+#\|+\|\|\|+\| +\| \| \|\|+\|+\|\|\|\|\|\|+\|\|\| \| Sbjct 360 DGSWRKGSTAGGCRNHAYTFWMNPQFVIR#LDEEDDDPDDNEVGCSFVVGLIQKNRRRLR 418
Coturnix coturnix	Query 361 MDGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 +\| \|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|+\|\|\|+ \|\|#\| \|\|\|+\| \| \| \|+\|\|\| \|+\|\|\|\|\|\|+\| Sbjct 364 FEGTWRRGSTAGGCRNNPATFWINPQFKIK#LLEEDDDPGDDEVACSFLVALMQKHRRRER 423
Danio rerio	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| \|\|\|+ + #\|+ \| \|+ \|+\|\|\| \|+\|\| \|\|++\| Sbjct 367 NGEWRRGSTAGGCRNYPATFWNNPQFKVA#LKHP---DSPGQSECSFLVALMQKDRRKKR 422
Bos taurus	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDG----ERGCTFLVGLIQKH 415 +\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+#\|\|\| \|+ \| \| \| \|\|+\|\|+ \|+\|\|\| Sbjct 372 EGTWRRGSTAGGCRNYPATFWVNPQFKIR#LEETDDPDPDDYGGRESGCSFLLALMQKH 429
Drosophila melanogaster	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 +\| \| \| \|\|\|\|\|\|\|+ +\|\|\| \|\|\|\|+\| #\| + \|\|+\|\|+\|+ \|\| +\| \|+\|\|+\|\| +\| Sbjct 386 EGEWTPGVTAGGCRNFLDTFWHNPQYIIT#LVDPDEEDEEGQ--CTVIVALMQKNRRSKR 442
Drosophila melanogaster	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 +\| \| \| \|\|\|\|\|\|\|+ +\|\|\| \|\|\|\|+\| #\| + \|\|+\|\|+\|+ \|\| +\| \|+\|\|+\|\| +\| Sbjct 409 EGEWTPGVTAGGCRNFLDTFWHNPQYIIT#LVDPDEEDEEGQ--CTVIVALMQKNRRSKR 465
Xenopus laevis	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 \|\|+\|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|+\|\|\|+ + #\| \|\|\|+\| +\| \| \|+\|\|\| \|+\|\|+\|\| +\| Sbjct 365 DGSWRRGSTAGGCRNNPATFWINPQFKVT#LLEEDDDPDDNELACSFLVALMQKNRRNER 423
Schistosoma japonicum	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 +\| \|+\| +\|\|\|\|\|\|+ +\|\|\|+\|\|\|\|++ #+\|+ \|\|+\|\| \| \| +\|\|\|+\|\|+\|\|+ \| Sbjct 16 EGCWKRRVSAGGCRNFLDTFWINPQYVVS#VEDPDENDE--ENKGTLIVGLMQKNRRKMR 72
Macaca fascicularis	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 +\| \| \|\| +\|\|\|\|\|\|+\|+\|\|\| \|\|\|\| +\|#\| \|\|\|+\| +\| \| \|+\|\|\| \|+\|\|+\|\|+ \| Sbjct 435 EGRWVRGCSAGGCRNFPDTFWTNPQYRLK#LLEEDDDPDDSEVICSFLVALMQKNRRKDR 493
Ovis aries	Query 362 DGNWRRGSTAGGCRNYPNTFWMNPQYLIK#LEEEDEDDEDGERGCTFLVGLIQKHRRRQR 420 + \| \|\| +\|\|\|\|\|\|+\|+\|\|\| \|\|\|\| +\|#\| \|\|\|+\| +\| \| \|+\|\|\| \|+\|\|+\|\|+ \| Sbjct 149 EARWVRGCSAGGCRNFPDTFWTNPQYRLK#LLEEDDDPDDSEVICSFLVALMQKNRRKDR 207

organism	matching
N/A	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422
Rattus norvegicus	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422
Mus musculus	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDEEDGERGCTFLVGLIQKHRRRQRKM 422
Homo sapiens	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDEEDGESGCTFLVGLIQKHRRRQRKM 422
Homo sapiens	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDEEDGESGCTFLVGLIQKHRRRQRKM 422
Macaca mulatta	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDEEDGESGCTFLVGLIQKHRRRQRKM 422
Xenopus tropicalis	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \| \|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|+\|\|\|\|#\| \|+\| +\| \| \|\|\|\|+\|\|\|\|\|\|+\|\|+\|\|\|\| Sbjct 363 GTWRRGSTAGGCRNFPETFWMNPQYVIKLE#EADDDPDDNEEGCTFIVGLIQKNRRKQRKM 422
Stizostedion vitreum vitreum	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\|\|++\|+\|\|#\|\|\|+\| \|\|\| \|\|+\|+\|\|\|\|\|\|+\|\|\| \|\|+ Sbjct 364 GSWRRGSTAGGCRNYPDTFWTNPQFVIRLE#EEDDDPADGELGCSFVVGLIQKNRRRARKL 423
Bos grunniens	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQ 413 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 75 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDQEDGESGCTFLVGLIQ 125
Tetraodon nigroviridis	Query 364 NWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|+\|\|\|++\|\|\|\|#\| \|+\| + + \|\|\|\|+\|\|\|+\|\|+\|\|\| \|\|\| Sbjct 370 TWRRGSTAGGCRNYPNSFWLNPQFVIKLE#EVDDDPGEDQEGCTFIVGLMQKNRRRLRKM 428
Gallus gallus	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\| +\| \| \|\|\|\|\|+\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 363 GNWRRGATAGGCRNYPNTFWTNPQYLIKLE#EEDEDPDDPEGGCTFLIGLIQKHRRKQRKM 422
Xenopus laevis	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDG-ERGCTFLVGLIQKHRRRQRK 421 \|+\|\|+\|\|\|\|\|\|\|\|\|+\| \|\|\|+\|\|\|+ \|\|\|\|#\|\|\| \|\|+ \| \| \|\|\|\|\|+ \|+\|\|++\|++\|+ Sbjct 373 GSWRKGSTAGGCRNFPATFWINPQFKIKLE#EEDGDDDYGSETGCTFLLALMQKNKRKERR 432 Query 422 M 422 Sbjct 433 F 433
Danio rerio	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRK 421 \|\|\|\| \|\|\|\|\|\|\|\|\| \| \|\| \|\|\|++\|\|\|\|#\| \|+\| \|\| \|\|\|\|\|\|\|\|+\|\| \|+ ++ Sbjct 363 GNWRVGSTAGGCRNNPATFCSNPQFMIKLE#EIDDDPHDGVNGCTFLVGLMQKDGRKDKR 421
Rattus norvegicus	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#E-EDEDDEDG-ERGCTFLVGLIQKH 415 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+\|\|#\| +\| \|\| \| \| \|\|+\|\|+ \|+\|\|\| Sbjct 373 GTWRRGSTAGGCRNYPATFWVNPQFKIRLE#EVDDADDYDSRESGCSFLLALMQKH 427
Canis familiaris	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDG---ERGCTFLVGLIQKH 415 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+\|\|#\| \|+ \|+\| \| \|\|+\|++ \|+\|\|\| Sbjct 370 GTWRRGSTAGGCRNYPATFWVNPQFKIRLE#ETDDVDDDYGGRESGCSFVLALMQKH 425
synthetic construct	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGER--GCTFLVGLIQKH 415 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+\|+#\| \|+ \|+ \|+\| \|\|+\|++ \|+\|\|\| Sbjct 373 GTWRRGSTAGGCRNYPATFWVNPQFKIRLD#ETDDPDDYGDRESGCSFVLALMQKH 427
Pongo pygmaeus	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGER--GCTFLVGLIQKH 415 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+\|+#\| \|+ \|+ \|+\| \|\|+\|++ \|+\|\|\| Sbjct 373 GTWRRGSTAGGCRNYPATFWVNPQFKIRLD#ETDDPDDYGDRESGCSFVLALMQKH 427
Sus scrofa	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EED--EDDEDG-ERGCTFLVGLIQKH 415 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+\|\|#\| \| \|\|\| \| \| \|\|+\|++ \|+\|\|\| Sbjct 373 GTWRRGSTAGGCRNYPATFWVNPQFKIRLE#ETDDPEDDYGGRESGCSFVLALMQKH 428
Gallus gallus	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDG---ERGCTFLVGLIQKHRRRQ 419 \|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \| \|\|#\| \|+\| +\| \| \|\|+\|\|+ \|+\|\|\|\|\|\|+ Sbjct 373 GSWRRGSTAGGCRNYPATFWINPQFKICLE#EVDDDGDDFGGREPGCSFLLALMQKHRRRE 432 Query 420 RK 421 \|+ Sbjct 433 RR 434
Oncorhynchus mykiss	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \|+\|\|+\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\|++\|+\|+#\|\|\|+\| +\| \| \|\|+\|+\|\|\|\|\|\|+\|\|\| \|\|+ Sbjct 361 GSWRKGSTAGGCRNHAYTFWMNPQFVIRLD#EEDDDPDDNEVGCSFVVGLIQKNRRRLRKV 420
Coturnix coturnix	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \| \|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|+\|\|\|+ \|\|\| #\|\|\|+\| \| \| \|+\|\|\| \|+\|\|\|\|\|\|+\|++ Sbjct 366 GTWRRGSTAGGCRNNPATFWINPQFKIKLL#EEDDDPGDDEVACSFLVALMQKHRRRERRV 425
Danio rerio	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRK 421 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| \|\|\|+ + \|+# \| \|+ \|+\|\|\| \|+\|\| \|\|++\|+ Sbjct 368 GEWRRGSTAGGCRNYPATFWNNPQFKVALK#HP---DSPGQSECSFLVALMQKDRRKKRR 423
Drosophila melanogaster	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \| \| \| \|\|\|\|\|\|\|+ +\|\|\| \|\|\|\|+\| \| #+ \|\|+\|\|+\|+ \|\| +\| \|+\|\|+\|\| +\| \| Sbjct 387 GEWTPGVTAGGCRNFLDTFWHNPQYIITLV#DPDEEDEEGQ--CTVIVALMQKNRRSKRNM 444
Drosophila melanogaster	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \| \| \| \|\|\|\|\|\|\|+ +\|\|\| \|\|\|\|+\| \| #+ \|\|+\|\|+\|+ \|\| +\| \|+\|\|+\|\| +\| \| Sbjct 410 GEWTPGVTAGGCRNFLDTFWHNPQYIITLV#DPDEEDEEGQ--CTVIVALMQKNRRSKRNM 467
Bos taurus	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDG----ERGCTFLVGLIQKH 415 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|+ \|+\|\|#\| \|+ \| \| \| \|\|+\|\|+ \|+\|\|\| Sbjct 373 GTWRRGSTAGGCRNYPATFWVNPQFKIRLE#ETDDPDPDDYGGRESGCSFLLALMQKH 429
Xenopus laevis	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRK 421 \|+\|\|\|\|\|\|\|\|\|\|\|\| \| \|\|\|+\|\|\|+ + \| #\|\|\|+\| +\| \| \|+\|\|\| \|+\|\|+\|\| +\|\| Sbjct 366 GSWRRGSTAGGCRNNPATFWINPQFKVTLL#EEDDDPDDNELACSFLVALMQKNRRNERK 424
Schistosoma japonicum	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRK 421 \| \|+\| +\|\|\|\|\|\|+ +\|\|\|+\|\|\|\|++ +\|#+ \|\|+\|\| \| \| +\|\|\|+\|\|+\|\|+ \|+ Sbjct 17 GCWKRRVSAGGCRNFLDTFWINPQYVVSVE#DPDENDE--ENKGTLIVGLMQKNRRKMRQ 73
Macaca fascicularis	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \| \| \|\| +\|\|\|\|\|\|+\|+\|\|\| \|\|\|\| +\|\| #\|\|\|+\| +\| \| \|+\|\|\| \|+\|\|+\|\|+ \|\|+ Sbjct 436 GRWVRGCSAGGCRNFPDTFWTNPQYRLKLL#EEDDDPDDSEVICSFLVALMQKNRRKDRKL 495
Ovis aries	Query 363 GNWRRGSTAGGCRNYPNTFWMNPQYLIKLE#EEDEDDEDGERGCTFLVGLIQKHRRRQRKM 422 \| \|\| +\|\|\|\|\|\|+\|+\|\|\| \|\|\|\| +\|\| #\|\|\|+\| +\| \| \|+\|\|\| \|+\|\|+\|\|+ \|\|+ Sbjct 150 ARWVRGCSAGGCRNFPDTFWTNPQYRLKLL#EEDDDPDDSEVICSFLVALMQKNRRKDRKL 209

organism	matching
N/A	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437
Rattus norvegicus	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437
Mus musculus	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 378 PNTFWMNPQYLIKLEEEDEDEEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437
Homo sapiens	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 378 PNTFWMNPQYLIKLEEEDEDEEDGESGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437
Macaca mulatta	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 378 PNTFWMNPQYLIKLEEEDEDEEDGESGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437
Homo sapiens	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 378 PNTFWMNPQYLIKLEEEDEDEEDGESGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437
Xenopus tropicalis	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \| \|\|\|\|\|\|\|\|+\|\|\|\|\| \|+\| +\| \| \|\|\|\|#+\|\|\|\|\|\|+\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 378 PETFWMNPQYVIKLEEADDDPDDNEEGCTF#IVGLIQKNRRKQRKMGEDMHTIGFAIYEVP 437
Stizostedion vitreum vitreum	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|+\|\|\| \|\|\|++\|+\|\|\|\|\|+\| \|\|\| \|\|+\|#+\|\|\|\|\|\|+\|\|\| \|\|+\|\|\|\|\|\|+\|\| \|\|+\|\| Sbjct 379 PDTFWTNPQFVIRLEEEDDDPADGELGCSF#VVGLIQKNRRRARKLGEDMHTVGFAIYQVP 438
Gallus gallus	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| +\| \| \|\|\|\|#\|+\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 378 PNTFWTNPQYLIKLEEEDEDPDDPEGGCTF#LIGLIQKHRRKQRKMGEDMHTIGFAIYEVP 437
Tetraodon nigroviridis	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|+\|\|+\|\|\|++\|\|\|\|\| \|+\| + + \|\|\|\|#+\|\|\|+\|\|+\|\|\| \|\|\|\|\|\|\| \|\|\|\| \|\|\|+\| Sbjct 384 PNSFWLNPQFVIKLEEVDDDPGEDQEGCTF#IVGLMQKNRRRLRKMGEDMETIGFAIYELP 443
Xenopus laevis	Query 378 PNTFWMNPQYLIKLEEEDEDDEDG-ERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEV 436 \| \|\|\|+\|\|\|+ \|\|\|\|\|\|\| \|\|+ \| \| \|\|\|\|#\|+ \|+\|\|++\|++\|+ \|+\|\| \|\|\|\| +\|\|\| Sbjct 388 PATFWINPQFKIKLEEEDGDDDYGSETGCTF#LLALMQKNKRKERRFGKDMETIGFAVYEV 447 Query 437 P 437 \| Query 437 P 437
Oncorhynchus mykiss	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|\|\|\|\|\|++\|+\|+\|\|\|+\| +\| \| \|\|+\|#+\|\|\|\|\|\|+\|\|\| \|\|+\|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 378 TFWMNPQFVIRLDEEDDDPDDNEVGCSF#VVGLIQKNRRRLRKVGEDMHTIGFAIYEVP 435
Danio rerio	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \| \|\| \|\|\|++\|\|\|\|\| \|+\| \|\| \|\|\|\|#\|\|\|\|+\|\| \|+ ++ \| \|++\|\|\|\| \|\|+\|\| Sbjct 378 PATFCSNPQFMIKLEEIDDDPHDGVNGCTF#LVGLMQKDGRKDKRFGRDLNTIGFAIYKVP 437
Coturnix coturnix	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \| \|\|\|+\|\|\|+ \|\|\| \|\|\|+\| \| \| \|+\|#\|\| \|+\|\|\|\|\|\|+\|++\| \|\|\|\|\|\|\| +\|\|\|\| Sbjct 381 PATFWINPQFKIKLLEEDDDPGDDEVACSF#LVALMQKHRRRERRVGGDMHTIGFAVYEVP 440
Gallus gallus	Query 378 PNTFWMNPQYLIKLEE---EDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIY 434 \| \|\|\|+\|\|\|+ \| \|\|\| + +\| \| \|\|+\|#\|+ \|+\|\|\|\|\|\|+\|+ \|+\|\| \|\|\|\| +\| Sbjct 388 PATFWINPQFKICLEEVDDDGDDFGGREPGCSF#LLALMQKHRRRERRYGKDMETIGFAVY 447 Query 435 EVP 437 \|\|\| Query 435 EVP 437
Xenopus laevis	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \| \|\|\|+\|\|\|+ + \| \|\|\|+\| +\| \| \|+\|#\|\| \|+\|\|+\|\| +\|\| \|+\|\|\|\|\|\|\| +\|\|\|\| Sbjct 381 PATFWINPQFKVTLLEEDDDPDDNELACSF#LVALMQKNRRNERKAGQDMHTIGFAVYEVP 440
Danio rerio	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \| \|\|\| \|\|\|+ + \|+ \| \|+ \|+\|#\|\| \|+\|\| \|\|++\|+ \|+\|\| \|\|\|\| \|\|\|\|\| Sbjct 383 PATFWNNPQFKVALKHPDSP---GQSECSF#LVALMQKDRRKKRREGQDMETIGFAIYEVP 439
synthetic construct	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGER--GCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYE 435 \| \|\|\|+\|\|\|+ \|+\|+\| \|+ \|+ \|+\| \|\|+\|#++ \|+\|\|\|\|\|\|+\|+ \| \|\| \|\|\|\| +\|\| Sbjct 388 PATFWVNPQFKIRLDETDDPDDYGDRESGCSF#VLALMQKHRRRERRFGRDMETIGFAVYE 447 Query 436 VP 437 \|\| Query 436 VP 437
Pongo pygmaeus	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGER--GCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYE 435 \| \|\|\|+\|\|\|+ \|+\|+\| \|+ \|+ \|+\| \|\|+\|#++ \|+\|\|\|\|\|\|+\|+ \| \|\| \|\|\|\| +\|\| Sbjct 388 PATFWVNPQFKIRLDETDDPDDYGDRESGCSF#VLALMQKHRRRERRFGRDMETIGFAVYE 447 Query 436 VP 437 \|\| Query 436 VP 437
Canis familiaris	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|+\|\|\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|#\|\| \|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\| Sbjct 383 PDTFWTNPQYRLKLLEEDDDPDDSEVICSF#LVALMQKNRRKDRKLGANLFTIGFAIYEVP 442
Bos taurus	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|+\|\|\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|#\|\| \|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\| Sbjct 383 PDTFWTNPQYRLKLLEEDDDPDDSEVICSF#LVALMQKNRRKDRKLGANLFTIGFAIYEVP 442
Ovis aries	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|+\|\|\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|#\|\| \|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\| Sbjct 452 PDTFWTNPQYRLKLLEEDDDPDDSEVICSF#LVALMQKNRRKDRKLGANLFTIGFAIYEVP 511
Sus scrofa	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|+\|\|\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|#\|\| \|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\| Sbjct 451 PDTFWTNPQYRLKLLEEDDDPDDSEVICSF#LVALMQKNRRKDRKLGANLFTIGFAIYEVP 510
Macaca fascicularis	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|+\|\|\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|#\|\| \|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\| Sbjct 451 PDTFWTNPQYRLKLLEEDDDPDDSEVICSF#LVALMQKNRRKDRKLGANLFTIGFAIYEVP 510
Rattus norvegicus	Query 378 PNTFWMNPQYLIKLEE-EDEDDEDG-ERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYE 435 \| \|\|\|+\|\|\|+ \|+\|\|\| +\| \|\| \| \| \|\|+\|#\|+ \|+\|\|\|\|\|\|+\|+ \| \|\| \|\|\|\| +\|+ Sbjct 388 PATFWVNPQFKIRLEEVDDADDYDSRESGCSF#LLALMQKHRRRERRFGRDMETIGFAVYQ 447 Query 436 VP 437 \|\| Query 436 VP 437
Oryctolagus cuniculus	Query 378 PNTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|+\|\|\| \|\|\|\| +\|\| \|\|\|+\| \|\| \| \|+\|#\|\| \|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\| Sbjct 383 PDTFWTNPQYRLKLLEEDDDPEDSEVICSF#LVALMQKNRRKDRKLGANLFTIGFAIYEVP 442
Schistosoma japonicum	Query 379 NTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 +\|\|\|+\|\|\|\|++ +\|+ \|\|+\|\| \| \| #+\|\|\|+\|\|+\|\|+ \|+ \| \|+ \|\|\|+ +\|++\| Sbjct 33 DTFWINPQYVVSVEDPDENDE--ENKGTL#IVGLMQKNRRKMRQEGADLLTIGYAVYKLP 89
Drosophila melanogaster	Query 379 NTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEV 436 +\|\|\| \|\|\|\|+\| \| + \|\|+\|\|+\|+ \|\| #+\| \|+\|\|+\|\| +\| \|\| + \|\|\|\| \|\| + Sbjct 403 DTFWHNPQYIITLVDPDEEDEEGQ--CTV#IVALMQKNRRSKRNMGMECLTIGFAIYSL 458
Drosophila melanogaster	Query 379 NTFWMNPQYLIKLEEEDEDDEDGERGCTF#LVGLIQKHRRRQRKMGEDMHTIGFGIYEV 436 +\|\|\| \|\|\|\|+\| \| + \|\|+\|\|+\|+ \|\| #+\| \|+\|\|+\|\| +\| \|\| + \|\|\|\| \|\| + Sbjct 426 DTFWHNPQYIITLVDPDEEDEEGQ--CTV#IVALMQKNRRSKRNMGMECLTIGFAIYSL 481

organism	matching
N/A	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439
Rattus norvegicus	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439
Mus musculus	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 380 TFWMNPQYLIKLEEEDEDEEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439
Homo sapiens	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 380 TFWMNPQYLIKLEEEDEDEEDGESGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439
Homo sapiens	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 380 TFWMNPQYLIKLEEEDEDEEDGESGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439
Macaca mulatta	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 380 TFWMNPQYLIKLEEEDEDEEDGESGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439
Xenopus tropicalis	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\|\|\|\|\|\|+\|\|\|\|\| \|+\| +\| \| \|\|\|\|+\|#\|\|\|\|\|+\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| + Sbjct 380 TFWMNPQYVIKLEEADDDPDDNEEGCTFIV#GLIQKNRRKQRKMGEDMHTIGFAIYEVPPQ 439
Stizostedion vitreum vitreum	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\| \|\|\|++\|+\|\|\|\|\|+\| \|\|\| \|\|+\|+\|#\|\|\|\|\|+\|\|\| \|\|+\|\|\|\|\|\|+\|\| \|\|+\|\|+\| Sbjct 381 TFWTNPQFVIRLEEEDDDPADGELGCSFVV#GLIQKNRRRARKLGEDMHTVGFAIYQVPDE 440
Tetraodon nigroviridis	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 +\|\|+\|\|\|++\|\|\|\|\| \|+\| + + \|\|\|\|+\|#\|\|+\|\|+\|\|\| \|\|\|\|\|\|\| \|\|\|\| \|\|\|+\|\|\| Sbjct 386 SFWLNPQFVIKLEEVDDDPGEDQEGCTFIV#GLMQKNRRRLRKMGEDMETIGFAIYELPEE 445
Gallus gallus	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| +\| \| \|\|\|\|\|+#\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| \| Sbjct 380 TFWTNPQYLIKLEEEDEDPDDPEGGCTFLI#GLIQKHRRKQRKMGEDMHTIGFAIYEVPPE 439
Xenopus laevis	Query 380 TFWMNPQYLIKLEEEDEDDEDG-ERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPE 438 \|\|\|+\|\|\|+ \|\|\|\|\|\|\| \|\|+ \| \| \|\|\|\|\|+# \|+\|\|++\|++\|+ \|+\|\| \|\|\|\| +\|\|\|\| Sbjct 390 TFWINPQFKIKLEEEDGDDDYGSETGCTFLL#ALMQKNKRKERRFGKDMETIGFAVYEVPR 449 Query 439 E 439 \| Query 439 E 439
Oncorhynchus mykiss	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\|\|\|\|\|++\|+\|+\|\|\|+\| +\| \| \|\|+\|+\|#\|\|\|\|\|+\|\|\| \|\|+\|\|\|\|\|\|\|\|\| \|\|\|\|\| + Sbjct 378 TFWMNPQFVIRLDEEDDDPDDNEVGCSFVV#GLIQKNRRRLRKVGEDMHTIGFAIYEVPSQ 437
Danio rerio	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\| \|\|\|++\|\|\|\|\| \|+\| \|\| \|\|\|\|\|\|#\|\|+\|\| \|+ ++ \| \|++\|\|\|\| \|\|+\|\|+\| Sbjct 380 TFCSNPQFMIKLEEIDDDPHDGVNGCTFLV#GLMQKDGRKDKRFGRDLNTIGFAIYKVPDE 439
Coturnix coturnix	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\|+\|\|\|+ \|\|\| \|\|\|+\| \| \| \|+\|\|\|# \|+\|\|\|\|\|\|+\|++\| \|\|\|\|\|\|\| +\|\|\|\|\|\| Sbjct 383 TFWINPQFKIKLLEEDDDPGDDEVACSFLV#ALMQKHRRRERRVGGDMHTIGFAVYEVPEE 442
Gallus gallus	Query 380 TFWMNPQYLIKLEE---EDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEV 436 \|\|\|+\|\|\|+ \| \|\|\| + +\| \| \|\|+\|\|+# \|+\|\|\|\|\|\|+\|+ \|+\|\| \|\|\|\| +\|\|\| Sbjct 390 TFWINPQFKICLEEVDDDGDDFGGREPGCSFLL#ALMQKHRRRERRYGKDMETIGFAVYEV 449 Query 437 PEE 439 \| \| Sbjct 450 PPE 452
Xenopus laevis	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\|+\|\|\|+ + \| \|\|\|+\| +\| \| \|+\|\|\|# \|+\|\|+\|\| +\|\| \|+\|\|\|\|\|\|\| +\|\|\|\|+\| Sbjct 383 TFWINPQFKVTLLEEDDDPDDNELACSFLV#ALMQKNRRNERKAGQDMHTIGFAVYEVPDE 442
Ovis aries	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|\|\|# \|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\|+\| Sbjct 2 TFWTNPQYRLKLLEEDDDPDDSEVICSFLV#ALMQKNRRKDRKLGANLFTIGFAIYEVPKE 61
Schistosoma japonicum	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\|+\|\|\|\|++ +\|+ \|\|+\|\| \| \| +\|#\|\|+\|\|+\|\|+ \|+ \| \|+ \|\|\|+ +\|++\|\|+ Sbjct 34 TFWINPQYVVSVEDPDENDE--ENKGTLIV#GLMQKNRRKMRQEGADLLTIGYAVYKLPEQ 91
Canis familiaris	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|\|\|# \|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\|+\| Sbjct 407 TFWTNPQYRLKLLEEDDDPDDSEVICSFLV#ALMQKNRRKDRKLGANLFTIGFAIYEVPKE 466
Bos taurus	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|\|\|# \|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\|+\| Sbjct 167 TFWTNPQYRLKLLEEDDDPDDSEVICSFLV#ALMQKNRRKDRKLGANLFTIGFAIYEVPKE 226
synthetic construct	Query 380 TFWMNPQYLIKLEEEDEDDEDGER--GCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|\|+\|\|\|+ \|+\|+\| \|+ \|+ \|+\| \|\|+\|++# \|+\|\|\|\|\|\|+\|+ \| \|\| \|\|\|\| +\|\|\|\| Sbjct 390 TFWVNPQFKIRLDETDDPDDYGDRESGCSFVL#ALMQKHRRRERRFGRDMETIGFAVYEVP 449 Query 438 EE 439 \| Sbjct 450 PE 451
Danio rerio	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\| \|\|\|+ + \|+ \| \|+ \|+\|\|\|# \|+\|\| \|\|++\|+ \|+\|\| \|\|\|\| \|\|\|\|\| \| Sbjct 385 TFWNNPQFKVALKHPDSP---GQSECSFLV#ALMQKDRRKKRREGQDMETIGFAIYEVPRE 441
Pongo pygmaeus	Query 380 TFWMNPQYLIKLEEEDEDDEDGER--GCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|\|+\|\|\|+ \|+\|+\| \|+ \|+ \|+\| \|\|+\|++# \|+\|\|\|\|\|\|+\|+ \| \|\| \|\|\|\| +\|\|\|\| Sbjct 390 TFWVNPQFKIRLDETDDPDDYGDRESGCSFVL#ALMQKHRRRERRFGRDMETIGFAVYEVP 449 Query 438 EE 439 \| Sbjct 450 PE 451
Sus scrofa	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|\|\|# \|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\|+\| Sbjct 453 TFWTNPQYRLKLLEEDDDPDDSEVICSFLV#ALMQKNRRKDRKLGANLFTIGFAIYEVPKE 512
Macaca fascicularis	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|\|\|# \|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\|+\| Sbjct 453 TFWTNPQYRLKLLEEDDDPDDSEVICSFLV#ALMQKNRRKDRKLGANLFTIGFAIYEVPKE 512
Oryctolagus cuniculus	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|\| \|\|\|\| +\|\| \|\|\|+\| \|\| \| \|+\|\|\|# \|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\|+\| Sbjct 385 TFWTNPQYRLKLLEEDDDPEDSEVICSFLV#ALMQKNRRKDRKLGANLFTIGFAIYEVPKE 444
Rattus norvegicus	Query 380 TFWMNPQYLIKLEE-EDEDDEDG-ERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|\|+\|\|\|+ \|+\|\|\| +\| \|\| \| \| \|\|+\|\|+# \|+\|\|\|\|\|\|+\|+ \| \|\| \|\|\|\| +\|+\|\| Sbjct 390 TFWVNPQFKIRLEEVDDADDYDSRESGCSFLL#ALMQKHRRRERRFGRDMETIGFAVYQVP 449 Query 438 EE 439 \| Sbjct 450 RE 451
Drosophila melanogaster	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEV 436 \|\|\| \|\|\|\|+\| \| + \|\|+\|\|+\|+ \|\| +\|# \|+\|\|+\|\| +\| \|\| + \|\|\|\| \|\| + Sbjct 404 TFWHNPQYIITLVDPDEEDEEGQ--CTVIV#ALMQKNRRSKRNMGMECLTIGFAIYSL 458
Drosophila melanogaster	Query 380 TFWMNPQYLIKLEEEDEDDEDGERGCTFLV#GLIQKHRRRQRKMGEDMHTIGFGIYEV 436 \|\|\| \|\|\|\|+\| \| + \|\|+\|\|+\|+ \|\| +\|# \|+\|\|+\|\| +\| \|\| + \|\|\|\| \|\| + Sbjct 427 TFWHNPQYIITLVDPDEEDEEGQ--CTVIV#ALMQKNRRSKRNMGMECLTIGFAIYSL 481

organism	matching
N/A	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440
Rattus norvegicus	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440
Mus musculus	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 381 FWMNPQYLIKLEEEDEDEEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440
Homo sapiens	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 381 FWMNPQYLIKLEEEDEDEEDGESGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440
Macaca mulatta	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 381 FWMNPQYLIKLEEEDEDEEDGESGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440
Homo sapiens	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 381 FWMNPQYLIKLEEEDEDEEDGESGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440
Xenopus tropicalis	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\|\|\|\|\|\|+\|\|\|\|\| \|+\| +\| \| \|\|\|\|+\|\|#\|\|\|\|+\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| +\| Sbjct 381 FWMNPQYVIKLEEADDDPDDNEEGCTFIVG#LIQKNRRKQRKMGEDMHTIGFAIYEVPPQL 440
Stizostedion vitreum vitreum	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\| \|\|\|++\|+\|\|\|\|\|+\| \|\|\| \|\|+\|+\|\|#\|\|\|\|+\|\|\| \|\|+\|\|\|\|\|\|+\|\| \|\|+\|\|+\| Sbjct 382 FWTNPQFVIRLEEEDDDPADGELGCSFVVG#LIQKNRRRARKLGEDMHTVGFAIYQVPDEF 441
Tetraodon nigroviridis	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|+\|\|\|++\|\|\|\|\| \|+\| + + \|\|\|\|+\|\|#\|+\|\|+\|\|\| \|\|\|\|\|\|\| \|\|\|\| \|\|\|+\|\|\| Sbjct 387 FWLNPQFVIKLEEVDDDPGEDQEGCTFIVG#LMQKNRRRLRKMGEDMETIGFAIYELPEE 445
Gallus gallus	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| +\| \| \|\|\|\|\|+\|#\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| \| Sbjct 381 FWTNPQYLIKLEEEDEDPDDPEGGCTFLIG#LIQKHRRKQRKMGEDMHTIGFAIYEVPPEF 440
Xenopus laevis	Query 381 FWMNPQYLIKLEEEDEDDEDG-ERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|+\|\|\|+ \|\|\|\|\|\|\| \|\|+ \| \| \|\|\|\|\|+ #\|+\|\|++\|++\|+ \|+\|\| \|\|\|\| +\|\|\|\| \| Sbjct 391 FWINPQFKIKLEEEDGDDDYGSETGCTFLLA#LMQKNKRKERRFGKDMETIGFAVYEVPRE 450 Query 440 L 440 Sbjct 451 F 451
Oncorhynchus mykiss	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\|\|\|\|\|++\|+\|+\|\|\|+\| +\| \| \|\|+\|+\|\|#\|\|\|\|+\|\|\| \|\|+\|\|\|\|\|\|\|\|\| \|\|\|\|\| + Sbjct 379 FWMNPQFVIRLDEEDDDPDDNEVGCSFVVG#LIQKNRRRLRKVGEDMHTIGFAIYEVPSQF 438
Danio rerio	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \| \|\|\|++\|\|\|\|\| \|+\| \|\| \|\|\|\|\|\|\|#\|+\|\| \|+ ++ \| \|++\|\|\|\| \|\|+\|\|+\|\| Sbjct 381 FCSNPQFMIKLEEIDDDPHDGVNGCTFLVG#LMQKDGRKDKRFGRDLNTIGFAIYKVPDEL 440
Coturnix coturnix	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|+\|\|\|+ \|\|\| \|\|\|+\| \| \| \|+\|\|\| #\|+\|\|\|\|\|\|+\|++\| \|\|\|\|\|\|\| +\|\|\|\|\|\| Sbjct 384 FWINPQFKIKLLEEDDDPGDDEVACSFLVA#LMQKHRRRERRVGGDMHTIGFAVYEVPEE 442
Gallus gallus	Query 381 FWMNPQYLIKLEE---EDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|+\|\|\|+ \| \|\|\| + +\| \| \|\|+\|\|+ #\|+\|\|\|\|\|\|+\|+ \|+\|\| \|\|\|\| +\|\|\|\| Sbjct 391 FWINPQFKICLEEVDDDGDDFGGREPGCSFLLA#LMQKHRRRERRYGKDMETIGFAVYEVP 450 Query 438 EE 439 \| Sbjct 451 PE 452
Xenopus laevis	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\|+\|\|\|+ + \| \|\|\|+\| +\| \| \|+\|\|\| #\|+\|\|+\|\| +\|\| \|+\|\|\|\|\|\|\| +\|\|\|\|+\| Sbjct 384 FWINPQFKVTLLEEDDDPDDNELACSFLVA#LMQKNRRNERKAGQDMHTIGFAVYEVPDEF 443
synthetic construct	Query 381 FWMNPQYLIKLEEEDEDDEDGER--GCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPE 438 \|\|+\|\|\|+ \|+\|+\| \|+ \|+ \|+\| \|\|+\|++ #\|+\|\|\|\|\|\|+\|+ \| \|\| \|\|\|\| +\|\|\|\| Sbjct 391 FWVNPQFKIRLDETDDPDDYGDRESGCSFVLA#LMQKHRRRERRFGRDMETIGFAVYEVPP 450 Query 439 EL 440 \|\| Query 439 EL 440
Pongo pygmaeus	Query 381 FWMNPQYLIKLEEEDEDDEDGER--GCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPE 438 \|\|+\|\|\|+ \|+\|+\| \|+ \|+ \|+\| \|\|+\|++ #\|+\|\|\|\|\|\|+\|+ \| \|\| \|\|\|\| +\|\|\|\| Sbjct 391 FWVNPQFKIRLDETDDPDDYGDRESGCSFVLA#LMQKHRRRERRFGRDMETIGFAVYEVPP 450 Query 439 EL 440 \|\| Query 439 EL 440
Canis familiaris	Query 381 FWMNPQYLIKLEEEDEDDED---GERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|+\|\|\|+ \|+\|\|\| \|+ \|+\| \| \|\|+\|++ #\|+\|\|\|\|\|\|+\|+ \| \|\| \|\|\|\| +\|\|\|\| Sbjct 388 FWVNPQFKIRLEETDDVDDDYGGRESGCSFVLA#LMQKHRRRERRFGRDMETIGFAVYEVP 447 Query 438 EEL 440 \|\| Sbjct 448 PEL 450
Ovis aries	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|\|\| #\|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\|+\| Sbjct 3 FWTNPQYRLKLLEEDDDPDDSEVICSFLVA#LMQKNRRKDRKLGANLFTIGFAIYEVPKE 61
Schistosoma japonicum	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEE 439 \|\|+\|\|\|\|++ +\|+ \|\|+\|\| \| \| +\|\|#\|+\|\|+\|\|+ \|+ \| \|+ \|\|\|+ +\|++\|\|+ Sbjct 35 FWINPQYVVSVEDPDENDE--ENKGTLIVG#LMQKNRRKMRQEGADLLTIGYAVYKLPEQ 91
Bos taurus	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|\|\| #\|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\|+\|+ Sbjct 168 FWTNPQYRLKLLEEDDDPDDSEVICSFLVA#LMQKNRRKDRKLGANLFTIGFAIYEVPKEM 227
Rattus norvegicus	Query 381 FWMNPQYLIKLEE-EDEDDEDG-ERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPE 438 \|\|+\|\|\|+ \|+\|\|\| +\| \|\| \| \| \|\|+\|\|+ #\|+\|\|\|\|\|\|+\|+ \| \|\| \|\|\|\| +\|+\|\| Sbjct 391 FWVNPQFKIRLEEVDDADDYDSRESGCSFLLA#LMQKHRRRERRFGRDMETIGFAVYQVPR 450 Query 439 EL 440 \|\| Query 439 EL 440
Danio rerio	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\| \|\|\|+ + \|+ \| \|+ \|+\|\|\| #\|+\|\| \|\|++\|+ \|+\|\| \|\|\|\| \|\|\|\|\| \| Sbjct 386 FWNNPQFKVALKHPDSP---GQSECSFLVA#LMQKDRRKKRREGQDMETIGFAIYEVPREF 442
Sus scrofa	Query 381 FWMNPQYLIKLEEED--EDDEDG-ERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVP 437 \|\|+\|\|\|+ \|+\|\|\| \| \|\|\| \| \| \|\|+\|++ #\|+\|\|\|\|\|\|+\|+ \| \|\| \|\|\|\| +\|\|\|\| Sbjct 391 FWVNPQFKIRLEETDDPEDDYGGRESGCSFVLA#LMQKHRRRERRFGRDMETIGFAVYEVP 450 Query 438 EEL 440 \|\| Sbjct 451 PEL 453
Macaca fascicularis	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\| \|\|\|\| +\|\| \|\|\|+\| +\| \| \|+\|\|\| #\|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\|+\|+ Sbjct 454 FWTNPQYRLKLLEEDDDPDDSEVICSFLVA#LMQKNRRKDRKLGANLFTIGFAIYEVPKEM 513
Oryctolagus cuniculus	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEVPEEL 440 \|\| \|\|\|\| +\|\| \|\|\|+\| \|\| \| \|+\|\|\| #\|+\|\|+\|\|+ \|\|+\| ++ \|\|\|\| \|\|\|\|\|+\|+ Sbjct 386 FWTNPQYRLKLLEEDDDPEDSEVICSFLVA#LMQKNRRKDRKLGANLFTIGFAIYEVPKEM 445
Drosophila melanogaster	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEV 436 \|\| \|\|\|\|+\| \| + \|\|+\|\|+\|+ \|\| +\| #\|+\|\|+\|\| +\| \|\| + \|\|\|\| \|\| + Sbjct 405 FWHNPQYIITLVDPDEEDEEGQ--CTVIVA#LMQKNRRSKRNMGMECLTIGFAIYSL 458
Drosophila melanogaster	Query 381 FWMNPQYLIKLEEEDEDDEDGERGCTFLVG#LIQKHRRRQRKMGEDMHTIGFGIYEV 436 \|\| \|\|\|\|+\| \| + \|\|+\|\|+\|+ \|\| +\| #\|+\|\|+\|\| +\| \|\| + \|\|\|\| \|\| + Sbjct 428 FWHNPQYIITLVDPDEEDEEGQ--CTVIVA#LMQKNRRSKRNMGMECLTIGFAIYSL 481

organism	matching
N/A	Query 394 EDEDDEDGERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 394 EDEDDEDGERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453
Rattus norvegicus	Query 394 EDEDDEDGERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 394 EDEDDEDGERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453
Mus musculus	Query 394 EDEDDEDGERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 394 EDEDEEDGERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLGKNFF 453
Homo sapiens	Query 404 GCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 404 GCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELSGQTNIHLSKNFF 453
Homo sapiens	Query 404 GCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 404 GCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELSGQTNIHLSKNFF 453
Macaca mulatta	Query 404 GCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 404 GCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELSGQTNIHLSKNFF 453
Gallus gallus	Query 404 GCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|\|\|\|\|+\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\| \|\|\|\|\| \| +\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 404 GCTFLIGLIQKHRRKQRKMG#EDMHTIGFAIYEVPPEFSGQTNIHLSKNFF 453
Xenopus tropicalis	Query 395 DEDDEDGERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNF 452 \|+\| +\| \| \|\|\|\|+\|\|\|\|\|\|+\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\| \|\|\|\|\| +\|\|\|\| +\| \|\|+++ Sbjct 395 DDDPDDNEEGCTFIVGLIQKNRRKQRKMG#EDMHTIGFAIYEVPPQLTGQKDIRLSRDY 452
Stizostedion vitreum vitreum	Query 394 EDEDDEDGERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|\|+\| \|\|\| \|\|+\|+\|\|\|\|\|\|+\|\|\| \|\|+\|#\|\|\|\|\|+\|\| \|\|+\|\|+\| \| \|+\|\|+\|\|+\| Sbjct 395 EDDDPADGELGCSFVVGLIQKNRRRARKLG#EDMHTVGFAIYQVPDEFRDQRNVHLNKNYF 454
Tetraodon nigroviridis	Query 394 EDEDDEDGE--RGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKN 451 \|+ \|\|+ \|\| \|\|\|\|+\|\|\|+\|\|+\|\|\| \|\|\|\|#\|\|\| \|\|\|\| \|\|\|+\|\|\| +\| \|+\|\| \|\| Sbjct 398 EEVDDDPGEDQEGCTFIVGLMQKNRRRLRKMG#EDMETIGFAIYELPEEYSGLKNVHLKKN 457 Query 452 FF 453 \|\| Query 452 FF 453
Oncorhynchus mykiss	Query 404 GCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|\|+\|+\|\|\|\|\|\|+\|\|\| \|\|+\|#\|\|\|\|\|\|\|\| \|\|\|\|\| + \|\| +\|\| \|\|+\| Sbjct 402 GCSFVVGLIQKNRRRLRKVG#EDMHTIGFAIYEVPSQFHGQREVHLDKNYF 451
Coturnix coturnix	Query 404 GCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|+\|\|\| \|+\|\|\|\|\|\|+\|++\|# \|\|\|\|\|\|\| +\|\|\|\|\|\| \| \|+\|\| \|+\|\| Sbjct 407 ACSFLVALMQKHRRRERRVG#GDMHTIGFAVYEVPEEAQGSQNVHLKKDFF 456
Xenopus laevis	Query 394 EDEDDEDG-ERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNF 452 \|\| \|\|+ \| \| \|\|\|\|\|+ \|+\|\|++\|++\|+ \|#+\|\| \|\|\|\| +\|\|\|\| \| \|\|+ +\|\| ++\| Sbjct 404 EDGDDDYGSETGCTFLLALMQKNKRKERRFG#KDMETIGFAVYEVPREFLGQSGVHLKRDF 463 Query 453 F 453 \| Query 453 F 453
Danio rerio	Query 404 GCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|\|+ ++\|\|\|\|\|+\|\|+ \|\| \|#\|\|\|\|\|\|\|+ \|\|\|\|\| + \|\| +\|\| \|\|+\| Sbjct 402 GCSVVIGLIQKNRRKMRKAG#EDMHTIGYAIYEVPSQFHGQKEVHLDKNYF 451
Xenopus laevis	Query 404 GCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|+\|\|\| \|+\|\|+\|\| +\|\| \|#+\|\|\|\|\|\|\| +\|\|\|\|+\| \| \|+\|\| \|+\|\| Sbjct 407 ACSFLVALMQKNRRNERKAG#QDMHTIGFAVYEVPDEFKGCQNVHLKKDFF 456
Gallus gallus	Query 402 ERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \| \|\|+\|\|+ \|+\|\|\|\|\|\|+\|+ \|#+\|\| \|\|\|\| +\|\|\|\| \| \| + +\|\| ++\|\| Sbjct 415 EPGCSFLLALMQKHRRRERRYG#KDMETIGFAVYEVPPEHVGHSGVHLQRDFF 466
Danio rerio	Query 398 DEDGERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \| \|+ \|+\|\|\| \|+\|\| \|\|++\|+ \|#+\|\| \|\|\|\| \|\|\|\|\| \| \|\|+ +\|\| ++\|\| Sbjct 400 DSPGQSECSFLVALMQKDRRKKRREG#QDMETIGFAIYEVPREFLGQSGVHLKRDFF 455
Ovis aries	Query 402 ERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \| \|\|+\|\|+ \|+\|\|\|\|\|\|+\|+ \|# \|\| \|\|\|\| +\|\|\|\| \|\| \|\| +\|\| ++\|\| Sbjct 28 ESGCSFLLALMQKHRRRERRFG#RDMETIGFAVYEVPPELVGQPAVHLKRDFF 79
synthetic construct	Query 394 EDEDDE-DGERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNF 452 +\| \|\| \| \| \|\|+\|++ \|+\|\|\|\|\|\|+\|+ \|# \|\| \|\|\|\| +\|\|\|\| \|\| \|\| +\|\| ++\| Sbjct 405 DDPDDYGDRESGCSFVLALMQKHRRRERRFG#RDMETIGFAVYEVPPELVGQPAVHLKRDF 464 Query 453 F 453 \| Query 453 F 453
Pongo pygmaeus	Query 394 EDEDDE-DGERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNF 452 +\| \|\| \| \| \|\|+\|++ \|+\|\|\|\|\|\|+\|+ \|# \|\| \|\|\|\| +\|\|\|\| \|\| \|\| +\|\| ++\| Sbjct 405 DDPDDYGDRESGCSFVLALMQKHRRRERRFG#RDMETIGFAVYEVPPELAGQPAVHLKRDF 464 Query 453 F 453 \| Query 453 F 453
Canis familiaris	Query 394 EDEDDEDG--ERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKN 451 +\| \|\|+ \| \| \|\|+\|++ \|+\|\|\|\|\|\|+\|+ \|# \|\| \|\|\|\| +\|\|\|\| \|\| \|\| +\|\| ++ Sbjct 402 DDVDDDYGGRESGCSFVLALMQKHRRRERRFG#RDMETIGFAVYEVPPELVGQPAVHLKRD 461 Query 452 FF 453 \|\| Query 452 FF 453
Bos taurus	Query 405 CTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|+\|\|\| \|+\|\|+\|\|+ \|\|+\|# ++ \|\|\|\| \|\|\|\|\|+\|+ \| \|\| \|+\|\| Sbjct 479 CSFLVALMQKNRRKDRKLG#ANLFTIGFAIYEVPKEMHGNKQ-HLQKDFF 526
Sus scrofa	Query 405 CTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|+\|\|\| \|+\|\|+\|\|+ \|\|+\|# ++ \|\|\|\| \|\|\|\|\|+\|+ \| \|\| \|+\|\| Sbjct 478 CSFLVALMQKNRRKDRKLG#ANLFTIGFAIYEVPKEMHGNKQ-HLQKDFF 525
Macaca fascicularis	Query 405 CTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|+\|\|\| \|+\|\|+\|\|+ \|\|+\|# ++ \|\|\|\| \|\|\|\|\|+\|+ \| \|\| \|+\|\| Sbjct 478 CSFLVALMQKNRRKDRKLG#ANLFTIGFAIYEVPKEMHGNRQ-HLQKDFF 525
Oryctolagus cuniculus	Query 405 CTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNFF 453 \|+\|\|\| \|+\|\|+\|\|+ \|\|+\|# ++ \|\|\|\| \|\|\|\|\|+\|+ \| \|\| \| \|\| Sbjct 410 CSFLVALMQKNRRKDRKLG#ANLFTIGFAIYEVPKEMHGNKQ-HLQKEFF 457
Rattus norvegicus	Query 394 EDEDDEDG-ERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSKNF 452 +\| \|\| \| \| \|\|+\|\|+ \|+\|\|\|\|\|\|+\|+ \|# \|\| \|\|\|\| +\|+\|\| \|\| \|\| +\|\| ++\| Sbjct 405 DDADDYDSRESGCSFLLALMQKHRRRERRFG#RDMETIGFAVYQVPRELAGQP-VHLKRDF 463 Query 453 F 453 \| Query 453 F 453
Schistosoma japonicum	Query 394 EDEDDEDGERGCTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYEVPEELTGQTNIHLSK 450 \|\| \|+ \| \| \| +\|\|\|+\|\|+\|\|+ \|+ \|# \|+ \|\|\|+ +\|++\|\|+ \| ++ \| Sbjct 46 EDPDENDEENKGTLIVGLMQKNRRKMRQEG#ADLLTIGYAVYKLPEQHQGTLDMKFFK 102
Sus scrofa domestica	Query 405 CTFLVGLIQKHRRRQRKMG#EDMHTIGFGIYE 435 \|+\|\|\| \|+\|\|+\|\|+ \|\|+\|# ++ \|\|\|\| \|\|\| Sbjct 5 CSFLVALMQKNRRKDRKLG#ANLFTIGFAIYE 35

organism	matching
N/A	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479
Rattus norvegicus	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479
Mus musculus	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| #\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLG#KNFFLTTRARERSDTFINLREVLNRFKLPP 479
Homo sapiens	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 420 RKMGEDMHTIGFGIYEVPEELSGQTNIHLS#KNFFLTNRARERSDTFINLREVLNRFKLPP 479
Homo sapiens	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 420 RKMGEDMHTIGFGIYEVPEELSGQTNIHLS#KNFFLTNRARERSDTFINLREVLNRFKLPP 479
Macaca mulatta	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 420 RKMGEDMHTIGFGIYEVPEELSGQTNIHLS#KNFFLTNRARERSDTFINLREVLNRFKLPP 479
Gallus gallus	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| \| +\|\|\|\|\|\|\|\|#\|\|\|\|\|\| +\|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 420 RKMGEDMHTIGFAIYEVPPEFSGQTNIHLS#KNFFLTNKAREKSNTFINLREVLNRFKLPA 479
Xenopus tropicalis	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| +\|\|\|\| +\| \|\|#+++ \| + \|\|+\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|\| Sbjct 420 RKMGEDMHTIGFAIYEVPPQLTGQKDIRLS#RDYILRNKIREKSDTYINLREVLNRFRLPP 479
Tetraodon nigroviridis	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\|\|\|\|\|\| \|\|\|\| \|\|\|+\|\|\| +\| \|+\|\| #\|\|\|\|\|++ \| \|\|+\|\|\|\|\|\|\|\| +\|\| \|\|\| Sbjct 426 RKMGEDMETIGFAIYELPEEYSGLKNVHLK#KNFFLSSYATARSETFINLREVCSRFCLPP 485
Danio rerio	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\| \|\|\|\|\|\|\|\|+ \|\|\|\|\| + \|\| +\|\| #\|\|+\|\|\| + \|\|+\|\|\|\|\|\|\|\| \|\|\|\|\|\| Sbjct 418 RKAGEDMHTIGYAIYEVPSQFHGQKEVHLD#KNYFLTHAQKARSETFINLREVSTRFKLPP 477
Oncorhynchus mykiss	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\|+\|\|\|\|\|\|\|\|\| \|\|\|\|\| + \|\| +\|\| #\|\|+\|\|\| + \|\|+\|\|+\|\|\|\|\| \|\|\|+\|\| Sbjct 418 RKVGEDMHTIGFAIYEVPSQFHGQREVHLD#KNYFLTHAQKARSETFVNLREVSTRFKMPP 477
Coturnix coturnix	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|++\| \|\|\|\|\|\|\| +\|\|\|\|\|\| \| \|+\|\| #\|+\|\|\| ++\| \|\|+\|\|\|\|\|\|\|\| \|+ +\|\|\| Sbjct 423 RRVGGDMHTIGFAVYEVPEEAQGSQNVHLK#KDFFLRNQSRARSETFINLREVSNQIRLPP 482
Stizostedion vitreum vitreum	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\|+\|\|\|\|\|\|+\|\| \|\|+\|\|+\| \| \|+\|\|+#\|\|+\|\| + \|\|+\|\|\|\|\|\|\|\| +\|\| \|\|\| Sbjct 421 RKLGEDMHTVGFAIYQVPDEFRDQRNVHLN#KNYFLRHASAARSETFINLREVCSRFCLPP 480
Xenopus laevis	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\| \|+\|\|\|\|\|\|\| +\|\|\|\|+\| \| \|+\|\| #\|+\|\|\| ++ \|\|+\|\|\|\|\|\|\|\| + \|\|\| Sbjct 423 RKAGQDMHTIGFAVYEVPDEFKGCQNVHLK#KDFFLRHQSCARSETFINLREVSTKIHLPP 482
Xenopus laevis	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|+ \|+\|\| \|\|\|\| +\|\|\|\| \| \|\|+ +\|\| #++\|\|\| +\| \|\|+ \|\|\|\|\|\|\| \| \|\|\|\| Sbjct 431 RRFGKDMETIGFAVYEVPREFLGQSGVHLK#RDFFLQNASRARSEQFINLREVSTRHKLPP 490
Danio rerio	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLP 478 \|+ \|+\|\| \|\|\|\| \|\|\|\|\| \| \|\|+ +\|\| #++\|\|\|\| + \|\|+ \|\|\|\|\|\|\| +\|\|+\|\| Sbjct 422 RREGQDMETIGFAIYEVPREFLGQSGVHLK#RDFFLTHASSARSELFINLREVSSRFRLP 480
Canis familiaris	Query 425 DMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\| \|\|\|\| +\|\|\|\| \|\| \|\| +\|\| #++\|\|\| +\| \|\|+ \|\|\|\|\|\|\| \|\|+\|\|\| Sbjct 435 DMETIGFAVYEVPPELVGQPAVHLK#RDFFLANSSRARSEQFINLREVSTRFRLPP 489
Gallus gallus	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|+ \|+\|\| \|\|\|\| +\|\|\|\| \| \| + +\|\| #++\|\|\|+ +\| \|\|+ \|\|\|\|\|\|\| \| +\|\|\| Sbjct 433 RRYGKDMETIGFAVYEVPPEHVGHSGVHLQ#RDFFLSNASRARSEQFINLREVSTRLRLPP 492
Pongo pygmaeus	Query 425 DMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\| \|\|\|\| +\|\|\|\| \|\| \|\| +\|\| #++\|\|\| +\| \|\|+ \|\|\|\|\|\|\| \|\|+\|\|\| Sbjct 437 DMETIGFAVYEVPPELAGQPAVHLK#RDFFLANASRARSEQFINLREVSTRFRLPP 491
synthetic construct	Query 425 DMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\| \|\|\|\| +\|\|\|\| \|\| \|\| +\|\| #++\|\|\| +\| \|\|+ \|\|\|\|\|\|\| \|\|+\|\|\| Sbjct 437 DMETIGFAVYEVPPELVGQPAVHLK#RDFFLANASRARSEQFINLREVSTRFRLPP 491
Bos taurus	Query 425 DMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\| \|\|\|\| +\|\|\|\| \|\| \|\| +\|\| #++\|\|\|+ +\| \|\|+ \|\|\|\|\|\|\| \|\|+\|\|\| Sbjct 439 DMETIGFAVYEVPPELMGQPAVHLK#RDFFLSNASRARSEQFINLREVSTRFRLPP 493
Ovis aries	Query 425 DMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\| \|\|\|\| +\|\|\|\| \|\| \|\| +\|\| #++\|\|\| +\| \|\|+ \|\|\|\|\|\|\| \|\|+\|\|\| Sbjct 51 DMETIGFAVYEVPPELVGQPAVHLK#RDFFLANASRARSEQFINLREVSTRFRLPP 105
Sus scrofa	Query 425 DMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\| \|\|\|\| +\|\|\|\| \|\| \|\| +\|\| #++\|\|\| +\| \|\|+ \|\|\|\|\|\|\| \|\|+\|\|\| Sbjct 438 DMETIGFAVYEVPPELVGQP-VHLK#RDFFLANASRARSEQFINLREVSTRFRLPP 491
Oryctolagus cuniculus	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\|+\| ++ \|\|\|\| \|\|\|\|\|+\|+ \| \|\| #\| \|\|\| ++ \|\| \|+\|\|+\|\|\| \|\|+\|\|\| Sbjct 425 RKLGANLFTIGFAIYEVPKEMHGNKQ-HLQ#KEFFLYNASKARSKTYINMREVSQRFRLPP 483
Macaca fascicularis	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\|+\| ++ \|\|\|\| \|\|\|\|\|+\|+ \| \|\| #\|+\|\|\| +\| \|\| \|+\|\|+\|\|\| \|\|+\|\|\| Sbjct 493 RKLGANLFTIGFAIYEVPKEMHGNRQ-HLQ#KDFFLYNASRARSKTYINMREVSQRFRLPP 551
Rattus norvegicus	Query 425 DMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\| \|\|\|\| +\|+\|\| \|\| \|\| +\|\| #++\|\|\| +\| +\|+ \|\|\|\|\|\|\| \|\| +\|\|\| Sbjct 437 DMETIGFAVYQVPRELAGQP-VHLK#RDFFLANASRAQSEHFINLREVSNRIRLPP 490
Canis familiaris	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 ++ \| \|\| \|\|\|+ \|\|+ \| + + \|\|+#\|+\|\| ++ \|\| \|\|\|\|\|\|\|\| +\|\|\|\|\|\| Sbjct 237 KRFGADMLTIGYAIYQSPSK-----DEHLN#KDFFRYHASQARSKTFINLREVSDRFKLPP 291
Oncorhynchus mykiss	Query 420 RKMGEDMHTIGFGIYEVPEELTGQTNIHLS#KNFFLTTRARERSDTFINLREVLNRFKLPP 479 \|\| \| \|+ \|\|\|\| +\|\| \| + \|\| #\|+\|\| ++ \|\| \|+\|\|+\|\|+ \|\| \|\|\| Sbjct 395 RKEGLDLQTIGFALYEAPAD-----EDHLG#KDFFRYNGSKARSKTYINVREISERFTLPP 449
Rattus sp.	Query 455 TTRARERSDTFINLREVLNRFKLPP 479 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 455 TTRARERSDTFINLREVLNRFKLPP 479

organism	matching
Rattus norvegicus	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491
N/A	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491
Mus musculus	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 432 GIYEVPEELTGQTNIHLGKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491
Homo sapiens	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\| Sbjct 432 GIYEVPEELSGQTNIHLSKNFFLTNRARER#SDTFINLREVLNRFKLPPGEYILVPSTFEP 491
Homo sapiens	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\| Sbjct 432 GIYEVPEELSGQTNIHLSKNFFLTNRARER#SDTFINLREVLNRFKLPPGEYILVPSTFEP 491
Macaca mulatta	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\| Sbjct 432 GIYEVPEELSGQTNIHLSKNFFLTNRARER#SDTFINLREVLNRFKLPPGEYILVPSTFEP 491
Gallus gallus	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\| \| +\|\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|+#\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|++\|\|\|\|\|\|\| Sbjct 432 AIYEVPPEFSGQTNIHLSKNFFLTNKAREK#SNTFINLREVLNRFKLPAGEYIIVPSTFEP 491
Xenopus tropicalis	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\| +\|\|\|\| +\| \|\|+++ \| + \|\|+#\|\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|++\|\|\|\|\|\| Sbjct 432 AIYEVPPQLTGQKDIRLSRDYILRNKIREK#SDTYINLREVLNRFRLPPGEYVILPSTFEP 491
Tetraodon nigroviridis	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|+\|\|\| +\| \|+\|\| \|\|\|\|\|++ \| \|#\|+\|\|\|\|\|\|\|\| +\|\| \|\|\|\|\|\|++\|\|\|\|\|\|\| Sbjct 438 AIYELPEEYSGLKNVHLKKNFFLSSYATAR#SETFINLREVCSRFCLPPGEYLIVPSTFEP 497
Coturnix coturnix	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 +\|\|\|\|\|\| \| \|+\|\| \|+\|\|\| ++\| \|#\|+\|\|\|\|\|\|\|\| \|+ +\|\|\|\|\|\|++\|\|\|\|\|\|\| Sbjct 435 AVYEVPEEAQGSQNVHLKKDFFLRNQSRAR#SETFINLREVSNQIRLPPGEYIVVPSTFEP 494
Danio rerio	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\|\|\| \|\| \|+\|\| \|+\|\|\|+ + \|#\|+\|\|\|\|\|\|\|\| \| +\|\|\|\|\|\|++\|\|\|\|\|\|\| Sbjct 435 AIYEVPEEYTGCQNVHLKKDFFLSNSSAAR#SETFINLREVSTRLRLPPGEYIIVPSTFEP 494
Oncorhynchus mykiss	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\| + \|\| +\|\| \|\|+\|\|\| + \|#\|+\|\|+\|\|\|\|\| \|\|\|+\|\|\|\|\|++\|\|\|\|\|\|\| Sbjct 430 AIYEVPSQFHGQREVHLDKNYFLTHAQKAR#SETFVNLREVSTRFKMPPGEYLIVPSTFEP 489
Canis familiaris	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 +\|\|\|\| \|\| \|\| +\|\| ++\|\|\| +\| \|#\|+ \|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\| Sbjct 442 AVYEVPPELVGQPAVHLKRDFFLANSSRAR#SEQFINLREVSTRFRLPPGEYVVVPSTFEP 501
Stizostedion vitreum vitreum	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|+\|\|+\| \| \|+\|\|+\|\|+\|\| + \|#\|+\|\|\|\|\|\|\|\| +\|\| \|\|\|\|\|\|++\|\|\|\|\|\|\| Sbjct 433 AIYQVPDEFRDQRNVHLNKNYFLRHASAAR#SETFINLREVCSRFCLPPGEYLIVPSTFEP 492
Xenopus laevis	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 +\|\|\|\| \| \|\|+ +\|\| ++\|\|\| +\| \|#\|+ \|\|\|\|\|\|\| \| \|\|\|\|\|\|\|++\|\|\|\|\|\|\| Sbjct 443 AVYEVPREFLGQSGVHLKRDFFLQNASRAR#SEQFINLREVSTRHKLPPGEYIVVPSTFEP 502
Danio rerio	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\| \| \|\|+ +\|\| ++\|\|\|\| + \|#\|+ \|\|\|\|\|\|\| +\|\|+\|\| \|\|\|++\|\|\|\|\|\|\| Sbjct 434 AIYEVPREFLGQSGVHLKRDFFLTHASSAR#SELFINLREVSSRFRLPAGEYIIVPSTFEP 493
Bos taurus	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 +\|\|\|\| \|\| \|\| +\|\| ++\|\|\|+ +\| \|#\|+ \|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\| Sbjct 446 AVYEVPPELMGQPAVHLKRDFFLSNASRAR#SEQFINLREVSTRFRLPPGEYVVVPSTFEP 505
synthetic construct	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 +\|\|\|\| \|\| \|\| +\|\| ++\|\|\| +\| \|#\|+ \|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\| Sbjct 444 AVYEVPPELVGQPAVHLKRDFFLANASRAR#SEQFINLREVSTRFRLPPGEYVVVPSTFEP 503
Pongo pygmaeus	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 +\|\|\|\| \|\| \|\| +\|\| ++\|\|\| +\| \|#\|+ \|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\| Sbjct 444 AVYEVPPELAGQPAVHLKRDFFLANASRAR#SEQFINLREVSTRFRLPPGEYVVVPSTFEP 503
Ovis aries	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 +\|\|\|\| \|\| \|\| +\|\| ++\|\|\| +\| \|#\|+ \|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\| Sbjct 58 AVYEVPPELVGQPAVHLKRDFFLANASRAR#SEQFINLREVSTRFRLPPGEYVVVPSTFEP 117
Sus scrofa	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 +\|\|\|\| \|\| \|\| +\|\| ++\|\|\| +\| \|#\|+ \|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\| Sbjct 445 AVYEVPPELVGQP-VHLKRDFFLANASRAR#SEQFINLREVSTRFRLPPGEYVVVPSTFEP 503
Xenopus laevis	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 +\|\|\|\|+\| \| \|+\|\| \|+\|\|\| ++ \|#\|+\|\|\|\|\|\|\|\| + \|\|\|\|\|\|++\|\|\|\|\|\|\| Sbjct 435 AVYEVPDEFKGCQNVHLKKDFFLRHQSCAR#SETFINLREVSTKIHLPPGEYIIVPSTFEP 494
Gallus gallus	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 +\|\|\|\| \| \| + +\|\| ++\|\|\|+ +\| \|#\|+ \|\|\|\|\|\|\| \| +\|\|\|\|\|\|++\|\|\|\|\|\|\| Sbjct 445 AVYEVPPEHVGHSGVHLQRDFFLSNASRAR#SEQFINLREVSTRLRLPPGEYIVVPSTFEP 504
Rattus norvegicus	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 +\|+\|\| \|\| \|\| +\|\| ++\|\|\| +\| +#\|+ \|\|\|\|\|\|\| \|\| +\|\|\|\|\|\|++\|\|\|\|\|\|\| Sbjct 444 AVYQVPRELAGQP-VHLKRDFFLANASRAQ#SEHFINLREVSNRIRLPPGEYIVVPSTFEP 502
Macaca fascicularis	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\|+\|+ \| \|\| \|+\|\|\| +\| \|#\| \|+\|\|+\|\|\| \|\|+\|\|\| \|\|\|+\|\|\|\|+\|\| Sbjct 505 AIYEVPKEMHGNRQ-HLQKDFFLYNASRAR#SKTYINMREVSQRFRLPPSEYVIVPSTYEP 563
Oryctolagus cuniculus	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\|+\|+ \| \|\| \| \|\|\| ++ \|#\| \|+\|\|+\|\|\| \|\|+\|\|\| \|\|\|+\|\|\|\|+\|\| Sbjct 437 AIYEVPKEMHGNKQ-HLQKEFFLYNASKAR#SKTYINMREVSQRFRLPPSEYVIVPSTYEP 495
Rattus sp.	Query 455 TTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 455 TTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491
Canis familiaris	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \|\|+ \| + + \|\|+\|+\|\| ++ \|#\| \|\|\|\|\|\|\|\| +\|\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\| Sbjct 249 AIYQSPSK-----DEHLNKDFFRYHASQAR#SKTFINLREVSDRFKLPPGEYILIPSTFEP 303
Caenorhabditis elegans	Query 442 GQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 \| \|\|\| \|\| ++ \|#\| \|\|\|\|\|\|+ \|\|++\|\|\| \|\|+\|\|\|\|\|\|\| Sbjct 710 GNNRGRLSKQFFAANKSAMR#SAAFINLREMTGRFRVPPGNYVVVPSTFEP 759
Oncorhynchus mykiss	Query 432 GIYEVPEELTGQTNIHLSKNFFLTTRARER#SDTFINLREVLNRFKLPPGEYVLVPSTFEP 491 +\|\| \| + \|\| \|+\|\| ++ \|#\| \|+\|\|+\|\|+ \|\| \|\|\|\| \|+\|\|\|+\|\|+\| Sbjct 407 ALYEAPAD-----EDHLGKDFFRYNGSKAR#SKTYINVREISERFTLPPGNYLLVPTTFQP 461

organism	matching
N/A	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492
Rattus norvegicus	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492
Mus musculus	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 433 IYEVPEELTGQTNIHLGKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492
Homo sapiens	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+ Sbjct 433 IYEVPEELSGQTNIHLSKNFFLTNRARERS#DTFINLREVLNRFKLPPGEYILVPSTFEPN 492
Homo sapiens	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+ Sbjct 433 IYEVPEELSGQTNIHLSKNFFLTNRARERS#DTFINLREVLNRFKLPPGEYILVPSTFEPN 492
Macaca mulatta	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+ Sbjct 433 IYEVPEELSGQTNIHLSKNFFLTNRARERS#DTFINLREVLNRFKLPPGEYILVPSTFEPN 492
Gallus gallus	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\| \| +\|\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|+\|#+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|++\|\|\|\|\|\|\|+ Sbjct 433 IYEVPPEFSGQTNIHLSKNFFLTNKAREKS#NTFINLREVLNRFKLPAGEYIIVPSTFEPN 492
Xenopus tropicalis	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\| +\|\|\|\| +\| \|\|+++ \| + \|\|+\|#\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|++\|\|\|\|\|\|\| Sbjct 433 IYEVPPQLTGQKDIRLSRDYILRNKIREKS#DTYINLREVLNRFRLPPGEYVILPSTFEPH 492
Coturnix coturnix	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 +\|\|\|\|\|\| \| \|+\|\| \|+\|\|\| ++\| \|\|#+\|\|\|\|\|\|\|\| \|+ +\|\|\|\|\|\|++\|\|\|\|\|\|\|\| Sbjct 436 VYEVPEEAQGSQNVHLKKDFFLRNQSRARS#ETFINLREVSNQIRLPPGEYIVVPSTFEPH 495
Danio rerio	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\| + \|\| +\|\| \|\|+\|\|\| + \|\|#+\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|++\|\|\|\|\|+\|\| Sbjct 431 IYEVPSQFHGQKEVHLDKNYFLTHAQKARS#ETFINLREVSTRFKLPPGEYLIVPSTFDPH 490
Oncorhynchus mykiss	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\| + \|\| +\|\| \|\|+\|\|\| + \|\|#+\|\|+\|\|\|\|\| \|\|\|+\|\|\|\|\|++\|\|\|\|\|\|\|\| Sbjct 431 IYEVPSQFHGQREVHLDKNYFLTHAQKARS#ETFVNLREVSTRFKMPPGEYLIVPSTFEPH 490
Tetraodon nigroviridis	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|+\|\|\| +\| \|+\|\| \|\|\|\|\|++ \| \|\|#+\|\|\|\|\|\|\|\| +\|\| \|\|\|\|\|\|++\|\|\|\|\|\|\|+ Sbjct 439 IYELPEEYSGLKNVHLKKNFFLSSYATARS#ETFINLREVCSRFCLPPGEYLIVPSTFEPN 498
Stizostedion vitreum vitreum	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|+\|\|+\| \| \|+\|\|+\|\|+\|\| + \|\|#+\|\|\|\|\|\|\|\| +\|\| \|\|\|\|\|\|++\|\|\|\|\|\|\|\| Sbjct 434 IYQVPDEFRDQRNVHLNKNYFLRHASAARS#ETFINLREVCSRFCLPPGEYLIVPSTFEPH 493
Canis familiaris	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 +\|\|\|\| \|\| \|\| +\|\| ++\|\|\| +\| \|\|#+ \|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+ Sbjct 443 VYEVPPELVGQPAVHLKRDFFLANSSRARS#EQFINLREVSTRFRLPPGEYVVVPSTFEPN 502
Bos taurus	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 +\|\|\|\| \|\| \|\| +\|\| ++\|\|\|+ +\| \|\|#+ \|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+ Sbjct 447 VYEVPPELMGQPAVHLKRDFFLSNASRARS#EQFINLREVSTRFRLPPGEYVVVPSTFEPN 506
Pongo pygmaeus	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 +\|\|\|\| \|\| \|\| +\|\| ++\|\|\| +\| \|\|#+ \|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+ Sbjct 445 VYEVPPELAGQPAVHLKRDFFLANASRARS#EQFINLREVSTRFRLPPGEYVVVPSTFEPN 504
synthetic construct	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 +\|\|\|\| \|\| \|\| +\|\| ++\|\|\| +\| \|\|#+ \|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+ Sbjct 445 VYEVPPELVGQPAVHLKRDFFLANASRARS#EQFINLREVSTRFRLPPGEYVVVPSTFEPN 504
Xenopus laevis	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 +\|\|\|\|+\| \| \|+\|\| \|+\|\|\| ++ \|\|#+\|\|\|\|\|\|\|\| + +\|\|\|\|\|\|++\|\|\|\|\|\|\|\| Sbjct 436 VYEVPDEFKGCQNVHLKKDFFLRHQSCARS#ETFINLREVSTQIRLPPGEYIIVPSTFEPH 495
Xenopus laevis	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 +\|\|\|\|+\| \| \|+\|\| \|+\|\|\| ++ \|\|#+\|\|\|\|\|\|\|\| + \|\|\|\|\|\|++\|\|\|\|\|\|\|\| Sbjct 436 VYEVPDEFKGCQNVHLKKDFFLRHQSCARS#ETFINLREVSTKIHLPPGEYIIVPSTFEPH 495
Danio rerio	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\| \| \|\|+ +\|\| ++\|\|\|\| + \|\|#+ \|\|\|\|\|\|\| +\|\|+\|\| \|\|\|++\|\|\|\|\|\|\|+ Sbjct 435 IYEVPREFLGQSGVHLKRDFFLTHASSARS#ELFINLREVSSRFRLPAGEYIIVPSTFEPN 494
Sus scrofa	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 +\|\|\|\| \|\| \|\| +\|\| ++\|\|\| +\| \|\|#+ \|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+ Sbjct 446 VYEVPPELVGQP-VHLKRDFFLANASRARS#EQFINLREVSTRFRLPPGEYVVVPSTFEPN 504
Ovis aries	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 +\|\|\|\| \|\| \|\| +\|\| ++\|\|\| +\| \|\|#+ \|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+ Sbjct 59 VYEVPPELVGQPAVHLKRDFFLANASRARS#EQFINLREVSTRFRLPPGEYVVVPSTFEPN 118
Gallus gallus	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 +\|\|\|\| \| \| + +\|\| ++\|\|\|+ +\| \|\|#+ \|\|\|\|\|\|\| \| +\|\|\|\|\|\|++\|\|\|\|\|\|\|+ Sbjct 446 VYEVPPEHVGHSGVHLQRDFFLSNASRARS#EQFINLREVSTRLRLPPGEYIVVPSTFEPN 505
Rattus norvegicus	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 +\|+\|\| \|\| \|\| +\|\| ++\|\|\| +\| +\|#+ \|\|\|\|\|\|\| \|\| +\|\|\|\|\|\|++\|\|\|\|\|\|\|+ Sbjct 445 VYQVPRELAGQP-VHLKRDFFLANASRAQS#EHFINLREVSNRIRLPPGEYIVVPSTFEPN 503
Macaca fascicularis	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\|+\|+ \| \|\| \|+\|\|\| +\| \|\|# \|+\|\|+\|\|\| \|\|+\|\|\| \|\|\|+\|\|\|\|+\|\|\| Sbjct 506 IYEVPKEMHGNRQ-HLQKDFFLYNASRARS#KTYINMREVSQRFRLPPSEYVIVPSTYEPH 564
Oryctolagus cuniculus	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\|+\|+ \| \|\| \| \|\|\| ++ \|\|# \|+\|\|+\|\|\| \|\|+\|\|\| \|\|\|+\|\|\|\|+\|\|\| Sbjct 438 IYEVPKEMHGNKQ-HLQKEFFLYNASKARS#KTYINMREVSQRFRLPPSEYVIVPSTYEPH 496
Rattus sp.	Query 455 TTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 455 TTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492
Canis familiaris	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 \|\|+ \| + + \|\|+\|+\|\| ++ \|\|# \|\|\|\|\|\|\|\| +\|\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\| Sbjct 250 IYQSPSK-----DEHLNKDFFRYHASQARS#KTFINLREVSDRFKLPPGEYILIPSTFEPH 304
Oncorhynchus mykiss	Query 433 IYEVPEELTGQTNIHLSKNFFLTTRARERS#DTFINLREVLNRFKLPPGEYVLVPSTFEPH 492 +\|\| \| + \|\| \|+\|\| ++ \|\|# \|+\|\|+\|\|+ \|\| \|\|\|\| \|+\|\|\|+\|\|+\|\| Sbjct 408 LYEAPAD-----EDHLGKDFFRYNGSKARS#KTYINVREISERFTLPPGNYLLVPTTFQPH 462

organism	matching
N/A	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498
Rattus norvegicus	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498
Mus musculus	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 439 ELTGQTNIHLGKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKDGDFC 498
Homo sapiens	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+\|+\|\|\|\| Sbjct 439 ELSGQTNIHLSKNFFLTNRARERSDTFINL#REVLNRFKLPPGEYILVPSTFEPNKDGDFC 498
Homo sapiens	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+\|+\|\|\|\| Sbjct 439 ELSGQTNIHLSKNFFLTNRARERSDTFINL#REVLNRFKLPPGEYILVPSTFEPNKDGDFC 498
Macaca mulatta	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+\|+\|\|\|\| Sbjct 439 ELSGQTNIHLSKNFFLTNRARERSDTFINL#REVLNRFKLPPGEYILVPSTFEPNKDGDFC 498
Gallus gallus	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 \| +\|\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|+\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\| \|\|\|++\|\|\|\|\|\|\|+ \|\|\|\|\| Sbjct 439 EFSGQTNIHLSKNFFLTNKAREKSNTFINL#REVLNRFKLPAGEYIIVPSTFEPNLNGDFC 498
Xenopus tropicalis	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 +\|\|\|\| +\| \|\|+++ \| + \|\|+\|\|\|+\|\|\|#\|\|\|\|\|\|\|+\|\|\|\|\|\|\|++\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 439 QLTGQKDIRLSRDYILRNKIREKSDTYINL#REVLNRFRLPPGEYVILPSTFEPHKVGDFC 498
Danio rerio	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 + \|\| +\|\| \|\|+\|\|\| + \|\|+\|\|\|\|\|#\|\|\| \|\|\|\|\|\|\|\|\|++\|\|\|\|\|+\|\|\|\|\|\|\|\| Sbjct 437 QFHGQKEVHLDKNYFLTHAQKARSETFINL#REVSTRFKLPPGEYLIVPSTFDPHKNGDFC 496
Tetraodon nigroviridis	Query 442 GQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 \|\| +\|\| \|\|\|\|\|+ + \|\|+\|\|\|\|\|#\|\|\| +\|\|\|\|\|\|\|\|\|+++\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 439 GQREVHLDKNFFLSHAQKARSETFINL#REVCSRFKLPPGEYLIIPSTFEPHLNGDFC 495
Oncorhynchus mykiss	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 + \|\| +\|\| \|\|+\|\|\| + \|\|+\|\|+\|\|#\|\|\| \|\|\|+\|\|\|\|\|++\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 437 QFHGQREVHLDKNYFLTHAQKARSETFVNL#REVSTRFKMPPGEYLIVPSTFEPHLNGDFC 496
Stizostedion vitreum vitreum	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 \| \| \|+\|\|+\|\|+\|\| + \|\|+\|\|\|\|\|#\|\|\| +\|\| \|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 440 EFRDQRNVHLNKNYFLRHASAARSETFINL#REVCSRFCLPPGEYLIVPSTFEPHKNGDFC 499
Coturnix coturnix	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \| \| \|+\|\| \|+\|\|\| ++\| \|\|+\|\|\|\|\|#\|\|\| \|+ +\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\| \|\| Sbjct 442 EAQGSQNVHLKKDFFLRNQSRARSETFINL#REVSNQIRLPPGEYIVVPSTFEPHKEADF 500
Canis familiaris	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \|\| \|\| +\|\| ++\|\|\| +\| \|\|+ \|\|\|\|#\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| Sbjct 449 ELVGQPAVHLKRDFFLANSSRARSEQFINL#REVSTRFRLPPGEYVVVPSTFEPNKEGDF 507
synthetic construct	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \|\| \|\| +\|\| ++\|\|\| +\| \|\|+ \|\|\|\|#\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| Sbjct 451 ELVGQPAVHLKRDFFLANASRARSEQFINL#REVSTRFRLPPGEYVVVPSTFEPNKEGDF 509
Bos taurus	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \|\| \|\| +\|\| ++\|\|\|+ +\| \|\|+ \|\|\|\|#\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| Sbjct 453 ELMGQPAVHLKRDFFLSNASRARSEQFINL#REVSTRFRLPPGEYVVVPSTFEPNKEGDF 511
Pongo pygmaeus	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \|\| \|\| +\|\| ++\|\|\| +\| \|\|+ \|\|\|\|#\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| Sbjct 451 ELAGQPAVHLKRDFFLANASRARSEQFINL#REVSTRFRLPPGEYVVVPSTFEPNKEGDF 509
Ovis aries	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 \|\| \|\| +\|\| ++\|\|\| +\| \|\|+ \|\|\|\|#\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| Sbjct 65 ELVGQPAVHLKRDFFLANASRARSEQFINL#REVSTRFRLPPGEYVVVPSTFEPNKEGDFV 124
Sus scrofa	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \|\| \|\| +\|\| ++\|\|\| +\| \|\|+ \|\|\|\|#\|\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| Sbjct 452 ELVGQP-VHLKRDFFLANASRARSEQFINL#REVSTRFRLPPGEYVVVPSTFEPNKEGDF 509
Xenopus laevis	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \| \| \|+\|\| \|+\|\|\| ++ \|\|+\|\|\|\|\|#\|\|\| + +\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\| \|\| Sbjct 442 EFKGCQNVHLKKDFFLRHQSCARSETFINL#REVSTQIRLPPGEYIIVPSTFEPHKEADF 500
Xenopus laevis	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \| \| \|+\|\| \|+\|\|\| ++ \|\|+\|\|\|\|\|#\|\|\| + \|\|\|\|\|\|++\|\|\|\|\|\|\|\|\| \|\| Sbjct 442 EFKGCQNVHLKKDFFLRHQSCARSETFINL#REVSTKIHLPPGEYIIVPSTFEPHKEADF 500
Gallus gallus	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \| \| + +\|\| ++\|\|\|+ +\| \|\|+ \|\|\|\|#\|\|\| \| +\|\|\|\|\|\|++\|\|\|\|\|\|\|++ \|\|\| Sbjct 452 EHVGHSGVHLQRDFFLSNASRARSEQFINL#REVSTRLRLPPGEYIVVPSTFEPNREGDF 510
Danio rerio	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \| \|\|+ +\|\| ++\|\|\|\| + \|\|+ \|\|\|\|#\|\|\| +\|\|+\|\| \|\|\|++\|\|\|\|\|\|\|+\| \|\| Sbjct 441 EFLGQSGVHLKRDFFLTHASSARSELFINL#REVSSRFRLPAGEYIIVPSTFEPNKEADF 499
Canis familiaris	Query 447 HLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDFC 498 \|\|+\|+\|\| ++ \|\| \|\|\|\|\|#\|\|\| +\|\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|+ \|\|\| Sbjct 259 HLNKDFFRYHASQARSKTFINL#REVSDRFKLPPGEYILIPSTFEPHQEADFC 310
Rattus norvegicus	Query 439 ELTGQTNIHLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \|\| \|\| +\|\| ++\|\|\| +\| +\|+ \|\|\|\|#\|\|\| \|\| +\|\|\|\|\|\|++\|\|\|\|\|\|\|+\| \|\|\| Sbjct 451 ELAGQP-VHLKRDFFLANASRAQSEHFINL#REVSNRIRLPPGEYIVVPSTFEPNKEGDF 508
Rattus sp.	Query 455 TTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 455 TTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPH 492
Macaca fascicularis	Query 447 HLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \|\| \|+\|\|\| +\| \|\| \|+\|\|+#\|\|\| \|\|+\|\|\| \|\|\|+\|\|\|\|+\|\|\|+ \|+\| Sbjct 519 HLQKDFFLYNASRARSKTYINM#REVSQRFRLPPSEYVIVPSTYEPHQEGEF 569
Oryctolagus cuniculus	Query 447 HLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \|\| \| \|\|\| ++ \|\| \|+\|\|+#\|\|\| \|\|+\|\|\| \|\|\|+\|\|\|\|+\|\|\|+ \|+\| Sbjct 451 HLQKEFFLYNASKARSKTYINM#REVSQRFRLPPSEYVIVPSTYEPHQEGEF 501
Oncorhynchus mykiss	Query 447 HLSKNFFLTTRARERSDTFINL#REVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \|\| \|+\|\| ++ \|\| \|+\|\|+#\|\|+ \|\| \|\|\|\| \|+\|\|\|+\|\|+\|\| \|\| Sbjct 417 HLGKDFFRYNGSKARSKTYINV#REISERFTLPPGNYLLVPTTFQPHDEADF 467

#	organism	max score	hits	top seq
1	N/A	134.00	15	A Chain A, Crystal Structure Analysis Of Rat M-Ca
2	Rattus norvegicus	134.00	11	calpain 2
3	Mus musculus	131.00	20	unnamed protein product
4	Homo sapiens	126.00	12	AF261089_1 calpain large polypeptide L2
5	Macaca mulatta	126.00	2	PREDICTED: calpain 2, large subunit
6	Homo sapiens	125.00	2	calpain 2, large subunit
7	Gallus gallus	108.00	3	calpain 2, (m/II) large subunit
8	Xenopus tropicalis	102.00	4	calpain 2, (m/II) large subunit
9	Danio rerio	101.00	5	calpain 2, (m/II) large subunit a
10	Tetraodon nigroviridis	100.00	7	unnamed protein product
11	Oncorhynchus mykiss	98.60	1	m-calpain
12	Stizostedion vitreum vitreum	97.40	1	putative calpain-like protein
13	Coturnix coturnix	91.30	1	quail calpain
14	Canis familiaris	90.10	22	PREDICTED: similar to Calpain-1 catalytic subunit
15	Bos taurus	89.70	5	calpain 1, (mu/I) large subunit
16	Pongo pygmaeus	89.70	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
17	synthetic construct	89.70	1	Homo sapiens calpain 1, (mu/I) large subunit
18	Ovis aries	87.00	3	calpain 1
19	Sus scrofa	86.30	4	calpain 1, large subunit
20	Xenopus laevis	85.10	3	hypothetical protein LOC398772
21	Canis familiaris	85.10	1	PREDICTED: similar to Calpain-9 (Digestive tract-
22	Gallus gallus	84.70	1	mu-calpain large subunit
23	Xenopus laevis	83.20	1	mu/m-calpain large subunit
24	Danio rerio	82.80	1	calpain 1, large subunit
25	Rattus norvegicus	82.40	1	calpain 1, large subunit
26	Rattus sp.	79.70	2	calpain II
27	Macaca fascicularis	78.60	1	CAN3_MACFA Calpain-3 (Calpain L3) (Calpain p94) (
28	Oryctolagus cuniculus	77.00	1	calpain 3, (p94)
29	Oncorhynchus mykiss	72.00	1	gill-specific calpain

organism	matching
N/A	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499
Rattus norvegicus	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499
Mus musculus	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\| Sbjct 440 LTGQTNIHLGKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKDGDFCI 499
Homo sapiens	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+\|+\|\|\|\|\| Sbjct 440 LSGQTNIHLSKNFFLTNRARERSDTFINLR#EVLNRFKLPPGEYILVPSTFEPNKDGDFCI 499
Macaca mulatta	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+\|+\|\|\|\|\| Sbjct 440 LSGQTNIHLSKNFFLTNRARERSDTFINLR#EVLNRFKLPPGEYILVPSTFEPNKDGDFCI 499
Homo sapiens	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+\|+\|\|\|\|\| Sbjct 440 LSGQTNIHLSKNFFLTNRARERSDTFINLR#EVLNRFKLPPGEYILVPSTFEPNKDGDFCI 499
Gallus gallus	Query 441 TGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 +\|\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|+\|+\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\| \|\|\|++\|\|\|\|\|\|\|+ \|\|\|\|\|+ Sbjct 441 SGQTNIHLSKNFFLTNKAREKSNTFINLR#EVLNRFKLPAGEYIIVPSTFEPNLNGDFCL 499
Xenopus tropicalis	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|\|\|\| +\| \|\|+++ \| + \|\|+\|\|\|+\|\|\|\|#\|\|\|\|\|\|+\|\|\|\|\|\|\|++\|\|\|\|\|\|\|\| \|\|\|\|+ Sbjct 440 LTGQKDIRLSRDYILRNKIREKSDTYINLR#EVLNRFRLPPGEYVILPSTFEPHKVGDFCL 499
Danio rerio	Query 442 GQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|\| +\|\| \|\|+\|\|\| + \|\|+\|\|\|\|\|\|#\|\| \|\|\|\|\|\|\|\|\|++\|\|\|\|\|+\|\|\|\|\|\|\|\|+ Sbjct 440 GQKEVHLDKNYFLTHAQKARSETFINLR#EVSTRFKLPPGEYLIVPSTFDPHKNGDFCV 497
Tetraodon nigroviridis	Query 442 GQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|\| +\|\| \|\|\|\|\|+ + \|\|+\|\|\|\|\|\|#\|\| +\|\|\|\|\|\|\|\|\|+++\|\|\|\|\|\|\| \|\|\|\|\|\| Sbjct 439 GQREVHLDKNFFLSHAQKARSETFINLR#EVCSRFKLPPGEYLIIPSTFEPHLNGDFCI 496
Oncorhynchus mykiss	Query 442 GQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|\| +\|\| \|\|+\|\|\| + \|\|+\|\|+\|\|\|#\|\| \|\|\|+\|\|\|\|\|++\|\|\|\|\|\|\|\| \|\|\|\|\|\| Sbjct 440 GQREVHLDKNYFLTHAQKARSETFVNLR#EVSTRFKMPPGEYLIVPSTFEPHLNGDFCI 497
Stizostedion vitreum vitreum	Query 443 QTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \| \|+\|\|+\|\|+\|\| + \|\|+\|\|\|\|\|\|#\|\| +\|\| \|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|+ Sbjct 444 QRNVHLNKNYFLRHASAARSETFINLR#EVCSRFCLPPGEYLIVPSTFEPHKNGDFCV 500
Coturnix coturnix	Query 442 GQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \| \|+\|\| \|+\|\|\| ++\| \|\|+\|\|\|\|\|\|#\|\| \|+ +\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\| \|\| + Sbjct 445 GSQNVHLKKDFFLRNQSRARSETFINLR#EVSNQIRLPPGEYIVVPSTFEPHKEADFIL 502
Canis familiaris	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \| \|\| +\|\| ++\|\|\| +\| \|\|+ \|\|\|\|\|#\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| + Sbjct 450 LVGQPAVHLKRDFFLANSSRARSEQFINLR#EVSTRFRLPPGEYVVVPSTFEPNKEGDFVL 509
Bos taurus	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \| \|\| +\|\| ++\|\|\|+ +\| \|\|+ \|\|\|\|\|#\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| + Sbjct 454 LMGQPAVHLKRDFFLSNASRARSEQFINLR#EVSTRFRLPPGEYVVVPSTFEPNKEGDFVL 513
Pongo pygmaeus	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \| \|\| +\|\| ++\|\|\| +\| \|\|+ \|\|\|\|\|#\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| + Sbjct 452 LAGQPAVHLKRDFFLANASRARSEQFINLR#EVSTRFRLPPGEYVVVPSTFEPNKEGDFVL 511
synthetic construct	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \| \|\| +\|\| ++\|\|\| +\| \|\|+ \|\|\|\|\|#\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| + Sbjct 452 LVGQPAVHLKRDFFLANASRARSEQFINLR#EVSTRFRLPPGEYVVVPSTFEPNKEGDFVL 511
Ovis aries	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \| \|\| +\|\| ++\|\|\| +\| \|\|+ \|\|\|\|\|#\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| + Sbjct 66 LVGQPAVHLKRDFFLANASRARSEQFINLR#EVSTRFRLPPGEYVVVPSTFEPNKEGDFVL 125
Sus scrofa	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \| \|\| +\|\| ++\|\|\| +\| \|\|+ \|\|\|\|\|#\|\| \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| + Sbjct 453 LVGQP-VHLKRDFFLANASRARSEQFINLR#EVSTRFRLPPGEYVVVPSTFEPNKEGDFVL 511
Xenopus laevis	Query 442 GQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \| \|+\|\| \|+\|\|\| ++ \|\|+\|\|\|\|\|\|#\|\| + +\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\| \|\| \| Sbjct 445 GCQNVHLKKDFFLRHQSCARSETFINLR#EVSTQIRLPPGEYIIVPSTFEPHKEADFII 502
Canis familiaris	Query 447 HLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|\|+\|+\|\| ++ \|\| \|\|\|\|\|\|#\|\| +\|\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|+ \|\|\|+ Sbjct 259 HLNKDFFRYHASQARSKTFINLR#EVSDRFKLPPGEYILIPSTFEPHQEADFCL 311
Gallus gallus	Query 442 GQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \| + +\|\| ++\|\|\|+ +\| \|\|+ \|\|\|\|\|#\|\| \| +\|\|\|\|\|\|++\|\|\|\|\|\|\|++ \|\|\| + Sbjct 455 GHSGVHLQRDFFLSNASRARSEQFINLR#EVSTRLRLPPGEYIVVPSTFEPNREGDFVL 512
Xenopus laevis	Query 442 GQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDF 497 \| \|+\|\| \|+\|\|\| ++ \|\|+\|\|\|\|\|\|#\|\| + \|\|\|\|\|\|++\|\|\|\|\|\|\|\|\| \|\| Sbjct 445 GCQNVHLKKDFFLRHQSCARSETFINLR#EVSTKIHLPPGEYIIVPSTFEPHKEADF 500
Danio rerio	Query 442 GQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|\|+ +\|\| ++\|\|\|\| + \|\|+ \|\|\|\|\|#\|\| +\|\|+\|\| \|\|\|++\|\|\|\|\|\|\|+\| \|\| + Sbjct 444 GQSGVHLKRDFFLTHASSARSELFINLR#EVSSRFRLPAGEYIIVPSTFEPNKEADFVL 501
Rattus norvegicus	Query 440 LTGQTNIHLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \| \|\| +\|\| ++\|\|\| +\| +\|+ \|\|\|\|\|#\|\| \|\| +\|\|\|\|\|\|++\|\|\|\|\|\|\|+\| \|\|\| + Sbjct 452 LAGQP-VHLKRDFFLANASRAQSEHFINLR#EVSNRIRLPPGEYIVVPSTFEPNKEGDFLL 510
Rattus sp.	Query 455 TTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPH 492 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 455 TTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPH 492
Macaca fascicularis	Query 447 HLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|\| \|+\|\|\| +\| \|\| \|+\|\|+\|#\|\| \|\|+\|\|\| \|\|\|+\|\|\|\|+\|\|\|+ \|+\| + Sbjct 519 HLQKDFFLYNASRARSKTYINMR#EVSQRFRLPPSEYVIVPSTYEPHQEGEFIL 571
Oryctolagus cuniculus	Query 447 HLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|\| \| \|\|\| ++ \|\| \|+\|\|+\|#\|\| \|\|+\|\|\| \|\|\|+\|\|\|\|+\|\|\|+ \|+\| + Sbjct 451 HLQKEFFLYNASKARSKTYINMR#EVSQRFRLPPSEYVIVPSTYEPHQEGEFIL 503
Oncorhynchus mykiss	Query 447 HLSKNFFLTTRARERSDTFINLR#EVLNRFKLPPGEYVLVPSTFEPHKNGDFCI 499 \|\| \|+\|\| ++ \|\| \|+\|\|+\|#\|+ \|\| \|\|\|\| \|+\|\|\|+\|\|+\|\| \|\| \| Sbjct 417 HLGKDFFRYNGSKARSKTYINVR#EISERFTLPPGNYLLVPTTFQPHDEADFII 469

organism	matching
N/A	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEEDIGD 532 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEEDIGD 532
Rattus norvegicus	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEEDIGD 532 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEEDIGD 532
Mus musculus	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEEDIGD 532 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\| \| Sbjct 473 NRFKLPPGEYVLVPSTFEPHKDGDFCIRVF#SEKKADYQAVDDEIEANIEEIDANEEDIDD 532
Homo sapiens	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEEDIGD 532 \|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|+\|\| + +\|+\|\| \| Sbjct 56 NRFKLPPGEYILVPSTFEPNKDGDFCIRVF#SEKKADYQAVDDEIEANLEEFDISEDDIDD 115
Homo sapiens	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEEDIGD 532 \|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|+\|\| + +\|+\|\| \| Sbjct 473 NRFKLPPGEYILVPSTFEPNKDGDFCIRVF#SEKKADYQAVDDEIEANLEEFDISEDDIDD 532
Macaca mulatta	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEEDIGD 532 \|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|+\|\| + +\|+\|\| \| Sbjct 473 NRFKLPPGEYILVPSTFEPNKDGDFCIRVF#SEKKADYQAVDDEIEANLEEFDISEDDIDD 532
Gallus gallus	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEEDI 530 \|\|\|\|\|\| \|\|\|++\|\|\|\|\|\|\|+ \|\|\|\|\|+\|\|\|#\|\|\| \|+ +\|\|\|\|\|\|\| \|\| \| +\|+\|\| Sbjct 473 NRFKLPAGEYIIVPSTFEPNLNGDFCLRVF#SEKNANSTVIDDEIEANFEETEIDEDDI 530
Danio rerio	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEEDI 530 \|\|\|\|\|\|\|\|\|++\|\|\|\|\|+\|\|\|\|\|\|\|\|+\|\|\|#\|\|\|+++ \| \|\| \|\|\|\|+++ +\|\|++ Sbjct 472 RFKLPPGEYLIVPSTFDPHKNGDFCVRVF#SEKQSEMQQCDDPIEANVDDEMVSEEEV 528
Tetraodon nigroviridis	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEEDI 530 +\|\|\|\|\|\|\|\|\|+++\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|+\|\| \| \|\| + \|\|+\|+ ++\|+ Sbjct 470 SRFKLPPGEYLIIPSTFEPHLNGDFCIRVF#SEKQADTQPCDDPVSANLEDETVADQDV 527
Stizostedion vitreum vitreum	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIE 521 +\|\| \|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|#\|\|\|+\|\|+\| +\|\| +\|+ +\| Sbjct 474 SRFCLPPGEYLIVPSTFEPHKNGDFCVRVF#SEKQADFQEMDDPVESRVE 522
Xenopus tropicalis	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEEDI 530 \|\|\|+\|\|\|\|\|\|\|++\|\|\|\|\|\|\|\| \|\|\|\|+\|\|\|#\|\|\| \|+ + \|\|\|\|+\|+\|+\| + \|\|+ \| Sbjct 473 NRFRLPPGEYVILPSTFEPHKVGDFCLRVF#SEKNAESKVPDDEIKADIDE-DVNEDAI 529
Oncorhynchus mykiss	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEEDI 530 \|\|\|+\|\|\|\|\|++\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|+ + + \|\| ++\|++++ ++ ++ Sbjct 472 RFKMPPGEYLIVPSTFEPHLNGDFCIRVF#SEKQTETRPCDDPVKADLDDETVSDAEV 528
Rattus norvegicus	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANI-EEIEANEEDIG 531 \|\| +\|\|\|\|\|\|++\|\|\|\|\|\|\|+\| \|\|\| +\| \|#\|\|\|\|\| \| +\|\|+\|+\|\|+ +\| +\|\|+\| Sbjct 484 NRIRLPPGEYIVVPSTFEPNKEGDFLLRFF#SEKKAGTQELDDQIQANLPDEKVLSEEEID 543 Query 532 D 532 \| Query 532 D 532
Bos taurus	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANI--EEIEANEE 528 \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| +\| \|#\|\|\| \| \| +\|\|+++\|\|+ \|++ + \|\| Sbjct 488 RFRLPPGEYVVVPSTFEPNKEGDFVLRFF#SEKSAGTQELDDQVQANLPDEQVLSEEE 544
Gallus gallus	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEE 522 \| +\|\|\|\|\|\|++\|\|\|\|\|\|\|++ \|\|\| +\|\|\|#\|\|\|+\|\| + +\|\|+\|\|\| + + Sbjct 487 RLRLPPGEYIVVPSTFEPNREGDFVLRVF#SEKRADTEEIDDKIEAKLPD 535
Canis familiaris	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANI--EEIEANEE 528 \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| +\| \|#\|\|\| \| + +\|\|+\|+\|\|+ \|++ + \|\| Sbjct 484 RFRLPPGEYVVVPSTFEPNKEGDFVLRFF#SEKSAGTEELDDQIQANLPDEQVLSEEE 540
Xenopus laevis	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANI-EEIEANEEDIGD 532 + +\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\| \|\| \|\|+\|#+\|\| +\| \| +\|+\|+ \|++ +\| \| \|+\|+ \| Sbjct 477 QIRLPPGEYIIVPSTFEPHKEADFIIRIF#TEKHSDTQELDEEVSADLPDEEELTEDDVDD 536
Sus scrofa	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANI--EEIEANEE 528 \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| +\| \|#\|\|\|\|\| \| +\|\|+++\| + \|++ + \|\| Sbjct 486 RFRLPPGEYVVVPSTFEPNKEGDFVLRFF#SEKKAGTQELDDQVQAILPDEQVLSEEE 542
Canis familiaris	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEI 523 +\|\|\|\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|+ \|\|\|+\|+\|#\|\|\|\|\| + +\| +++ ++ \|+ Sbjct 285 DRFKLPPGEYILIPSTFEPHQEADFCLRIF#SEKKAITRDMDGDVDIDLPEL 335
Pongo pygmaeus	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANI--EEIEANEE 528 \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| +\| \|#\|\|\| \| +\|\|+\|+\|\|+ \|++ + \|\| Sbjct 486 RFRLPPGEYVVVPSTFEPNKEGDFVLRFF#SEKSAGTAELDDQIQANLPDEQVLSEEE 542
Coturnix coturnix	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANI-EEIEANEEDIG 531 \|+ +\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\| \|\| +\|\|\|#+\|\|++\| +\|+\|\| \|++ +\| \| ++\|\| Sbjct 476 NQIRLPPGEYIVVPSTFEPHKEADFILRVF#TEKQSDTAELDEEISADLADEEEITDDDIE 535 Query 532 D 532 \| Query 532 D 532
Ovis aries	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANI--EEIEANEE 528 \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| +\| \|#\|\|\| \| \| +\|\|+++\|\|+ \|++ + \|\| Sbjct 99 TRFRLPPGEYVVVPSTFEPNKEGDFVLRFF#SEKSAGTQELDDQVQANLPDEQVLSEEE 156
Xenopus laevis	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANI-EEIEANEEDIGD 532 + \|\|\|\|\|\|++\|\|\|\|\|\|\|\|\| \|\| +\|+\|#+\|\| +\| \| +\|+\|+ \|++ \|\| \| \|+\|+ \| Sbjct 477 KIHLPPGEYIIVPSTFEPHKEADFIMRIF#TEKHSDIQELDEEVTADLPEEEELTEDDVDD 536
synthetic construct	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANI--EEIEANEE 528 \|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\| \|\|\| +\| \|#\|\|\| \| +\|\|+\|+\|\|+ \|++ + \|\| Sbjct 486 RFRLPPGEYVVVPSTFEPNKEGDFVLRFF#SEKSAGTVELDDQIQANLPDEQVLSEEE 542
Danio rerio	Query 473 NRFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEEIEANEE 528 +\|\|+\|\| \|\|\|++\|\|\|\|\|\|\|+\| \|\| +\|\|\|#\|\|\| \|+ + +\|\|++ \| \| \| + +\| Sbjct 475 SRFRLPAGEYIIVPSTFEPNKEADFVLRVF#SEKPANSEEMDDKVMAEIPEEQRLDE 530
Oryctolagus cuniculus	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEAN 519 \|\|+\|\|\| \|\|\|+\|\|\|\|+\|\|\|+ \|+\| +\|\|\|#\|\|\|+ + \|++ \| + Sbjct 478 RFRLPPSEYVIVPSTYEPHQEGEFILRVF#SEKRNLSEEVENTISVD 523
Drosophila melanogaster	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEI 516 \|\|\|\|\|\|\| \|++\|\|\|\|\|+\|++ \|+\| \|\|\|\|#\|\| + + + \|\|\|+ Sbjct 688 RFKLPPGHYLIVPSTFDPNEEGEFIIRVF#SETRNNMEENDDEV 730
Drosophila melanogaster	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEI 516 \|\|\|\|\|\|\| \|++\|\|\|\|\|+\|++ \|+\| \|\|\|\|#\|\| + + + \|\|\|+ Sbjct 688 RFKLPPGHYLIVPSTFDPNEEGEFIIRVF#SETRNNMEENDDEV 730
Drosophila pseudoobscura	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEI 516 \|\|\|\|\|\|\| \|++\|\|\|\|\|+\|++ \|+\| \|\|\|\|#\|\| + + \|\|\|+ Sbjct 694 RFKLPPGHYLIVPSTFDPNEEGEFIIRVF#SETMNNMEENDDEV 736
Macaca fascicularis	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEAN 519 \|\|+\|\|\| \|\|\|+\|\|\|\|+\|\|\|+ \|+\| +\|\|\|#\|\|\|+ + \|++ \| + Sbjct 546 RFRLPPSEYVIVPSTYEPHQEGEFILRVF#SEKRNLSEEVENTISVD 591
Oncorhynchus mykiss	Query 474 RFKLPPGEYVLVPSTFEPHKNGDFCIRVF#SEKKADYQTVDDEIEANIEE 522 \|\| \|\|\|\| \|+\|\|\|+\|\|+\|\| \|\| \|\|+\|#\|\|\| \| + + ++\|++ + Sbjct 444 RFTLPPGNYLLVPTTFQPHDEADFIIRIF#SEKAAGTMEMGNTVDADLPD 492