CaMPDB :: Substrate

SB0140 : TAR DNA-binding protein 43, TDP-43

* Basic Information

Organism	Homo sapiens (human)
Protein Names	TAR DNA-binding protein 43 [Homo sapiens]; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TDP-43
Gene Names	TARDBP; TDP43; TAR DNA binding protein
Gene Locus	1p36.22; chromosome 1
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_031401	NM_007375	23435	Q13148	605078	HGNC:11571	05466	hsa:23435

* Information From OMIM

Description: The TARDBP gene encodes the 43-kD TAR DNA-binding protein, which was originally identified as a transcriptional repressor that binds to TAR DNA of human immunodeficiency virus type 1. It is also involved in regulation of gene expression and splicing (summary by Benajiba et al., 2009).

» More (1) « Hide Below

* Structure Information

1. Primary Information

Length: 414 aa

Average Mass: 44.739 kDa

Monoisotopic Mass: 44.711 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
RNA recognition motif 1.	74	88	29.0	0.0
DNA ligase IV 1.	115	125	20.0	0.2
DNA ligase IV 2.	199	213	17.0	0.1
--- cleavage 229 ---
--- cleavage 243 ---
--- cleavage 286 ---
--- cleavage 295 ---
--- cleavage 297 ---
--- cleavage 302 ---
--- cleavage 303 ---
--- cleavage 304 ---
--- cleavage 324 ---
RNA recognition motif (a.k.a. RRM, RBD, or RNP domain) 1.	324	338	47.0	0.0
--- cleavage 324 (inside RNA recognition motif (a.k.a. RRM, RBD, or RNP domain) 324..338) ---

3. Sequence Information

Fasta Sequence: SB0140.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1WF0 (NMR; -; A=193-267), 2CQG (NMR; -; A=96-185), 4BS2 (NMR; -; A=102-269), 4IUF (X-ray; 275 A; A=103-179)

* Cleavage Information

10 [sites] cleaved by Calpain 1

Source Reference: [PubMed ID: 23250437] Yamashita T, Hideyama T, Hachiga K, Teramoto S, Takano J, Iwata N, Saido TC, Kwak S, A role for calpain-dependent cleavage of TDP-43 in amyotrophic lateral sclerosis pathology. Nat Commun. 2012;3:1307. doi: 10.1038/ncomms2303.

Cleavage sites (±10aa)

[Site 1] VFIPKPFRAF²²⁹-AFVTFADDQI

Phe²²⁹ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Val²²⁰	Phe²²¹	Ile²²²	Pro²²³	Lys²²⁴	Pro²²⁵	Phe²²⁶	Arg²²⁷	Ala²²⁸	Phe²²⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala²³⁰	Phe²³¹	Val²³²	Thr²³³	Phe²³⁴	Ala²³⁵	Asp²³⁶	Asp²³⁷	Gln²³⁸	Ile²³⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
EDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISN

#	organism	max score	hits	top seq
1	N/A	123.00	4	A Chain A, Solution Structure Of Rrm Domain In Ta
2	Bos taurus	119.00	2	TAR DNA binding protein
3	Tetraodon nigroviridis	117.00	3	unnamed protein product
4	Mus musculus	116.00	15	unnamed protein product
5	Xenopus laevis	116.00	2	mitotic phosphoprotein 39
6	Danio rerio	116.00	2	TAR DNA binding protein
7	Danio rerio	114.00	1	TAR DNA binding protein, like
8	Rattus norvegicus	111.00	1	TAR DNA binding protein
9	Drosophila melanogaster	102.00	3	TAR-binding protein
10	Drosophila melanogaster	102.00	2	TBPH CG10327-PB, isoform B
11	Caenorhabditis briggsae AF16	48.10	2	Hypothetical protein CBG03235
12	Caenorhabditis elegans	44.30	4	Temporarily Assigned Gene name family member (tag
13	Arabidopsis thaliana	42.70	7	RNA-binding like protein
14	Schistosoma japonicum	40.40	4	RNA-binding protein
15	Arabidopsis thaliana	39.70	14	AC013483_15 putative RNA-binding protein
16	Physcomitrella patens	39.30	1	glycine-rich RNA-binding protein
17	Homo sapiens	38.90	10	similar to Splicing factor, arginine/serine-rich,
18	Aspergillus nidulans FGSC A4	38.90	1	hypothetical protein AN6331.2
19	Oryza sativa (japonica cultivar-group)	38.50	3	Os08g0492100
20	Brassica oleracea	38.50	1	hypothetical protein 25.t00002
21	Theileria parva strain Muguga	38.10	2	hypothetical protein TP03_0515
22	Nicotiana tabacum	38.10	2	DNA binding protein ACBF
23	Yarrowia lipolytica	38.10	1	hypothetical protein
24	Ashbya gossypii ATCC 10895	38.10	1	ADL160Wp
25	Solanum tuberosum	38.10	1	DNA binding protein ACBF-like
26	Gallus gallus	38.10	1	splicing factor, arginine/serine-rich 2
27	Bombyx mori	37.70	2	Bmsqd-2
28	Strongylocentrotus purpuratus	37.70	1	PREDICTED: similar to arginine/serine-rich splici
29	Solanum tuberosum	37.70	1	putative glycine-rich RNA binding protein-like
30	Macaca mulatta	37.40	1	PREDICTED: similar to FUS interacting protein (se
31	Medicago sativa	36.60	1	AF191305_1 glycine-rich RNA binding protein
32	Nitrococcus mobilis Nb-231	35.80	1	RNA-binding protein
33	Coxiella burnetii RSA 493	35.80	1	nucleic acid binding domain protein
34	Ciona intestinalis	32.30	1	Tardbp protein
35	Drosophila pseudoobscura	32.30	1	GA10247-PA
36	Xenopus tropicalis	31.60	2	TADBP_XENTR TAR DNA-binding protein 43 (TDP-43) g
37	Brassica oleracea	31.60	1	RNA recognition motif. (a.k.a. RRM, RBD, or RNP d
38	Oryza sativa Japonica Group	31.20	1	putative RNA-binding like protein
39	Canis familiaris	31.20	1	PREDICTED: similar to TAR DNA binding protein iso

organism	matching
N/A	Query 201 DMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHISN 259 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 201 DMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHISN 259
Bos taurus	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHISN 259 \|\|\|\| \|\|\|\|+\|\| \|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 200 EDMTADELRQFFCQYGEVVDVFIPKPFRAF#AFVTFADDQVAQSLCGEDLIIKGISVHISN 259
Tetraodon nigroviridis	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHISN 259 \|\|\|\| \|+\|\|+\|\| \|\|\|+\| \|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 270 EDMTTDDLRQFFMQYGEVTDVFIPKPFRAF#AFVTFADDQVAQSLCGEDLIIKGVSVHISN 329
Mus musculus	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHISN 259 \|\|\|\| +\|\|++\|\| \|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 200 EDMTAEELQQFFCQYGEVVDVFIPKPFRAF#AFVTFADDKVAQSLCGEDLIIKGISVHISN 259
Xenopus laevis	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHIS 258 \|\|\|+ +\|\|\|+\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|#+\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|+\| Sbjct 199 EDMSAEELRQFFSQYGEVVDVFIPKPFRAF#SFVTFADDQVAQSLCGEDLIIKGVSVHVS 257
Danio rerio	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHISN 259 \|\|\|\| \|\|\|\|+\|\| \|\|\|+\| \|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|+\| +\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 206 EDMTADELRQFFMQYGEVTDVFIPKPFRAF#AFVTFADDQVAAALCGEDLIIKGVSVHISN 265
Danio rerio	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHISN 259 \|\|\|\| \|\|\|\|+\|\| \|\|\|+\| \|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| Sbjct 206 EDMTADELRQFFMQYGEVTDVFIPKPFRAF#AFVTFADDQVAQSLCGEDLIIKGTSVHISN 265
Rattus norvegicus	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHISN 259 \|\|\|\| +\|\|++\|\| \|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 200 EDMTAEELQQFFCQYGEVVDVFIPKPFRAF#AFVTFADDKVAQSLCGEDLIIKGISVHISN 259
Drosophila melanogaster	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHISN 259 \|\|+ \|+\|\|\|+\|\|++\|+\| \|\|\|\|\|+\|\|\|\|\|#+\|\|\|\| \| +\|\|\|\|\|\|\|\| \|\|\|\|+\|\|\|+\|\| Sbjct 201 EDINSDDLREYFSKFGEVTDVFIPRPFRAF#SFVTFLDPDVAQSLCGEDHIIKGVSVHVSN 260
Drosophila melanogaster	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHISN 259 \|\|+ \|+\|\|\|+\|\|++\|+\| \|\|\|\|\|+\|\|\|\|\|#+\|\|\|\| \| +\|\|\|\|\|\|\|\| \|\|\|\|+\|\|\|+\|\| Sbjct 201 EDINSDDLREYFSKFGEVTDVFIPRPFRAF#SFVTFLDPDVAQSLCGEDHIIKGVSVHVSN 260
Caenorhabditis briggsae AF16	Query 204 EDELREFFS-------QYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGE-DLIIKGISV 255 \|++\|\|+ \| + \| \|\|\|\|\|\|\|\|\| \|#\|\|\|\| + + \|+\|+ + \| + \|+\|\| Sbjct 272 ENQLRKVFGDEAKVYVETAVVTDVFIPKPFRGF#AFVTLSSAEAAESIVSKGSLTVNGLSV 331 Query 256 HIS 258 +\| Sbjct 332 GLS 334
Caenorhabditis elegans	Query 200 EDMTEDELREFFS-------QYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGE-DLIIK 251 + + \| +\|\|+ \| + \| \|\|\|\|\|\|\|\|\| \|#\|\|\|\| + + \|+ + + \| + Sbjct 268 DKVDEHQLRKVFGDEAKSYIETAVVTDVFIPKPFRGF#AFVTLSSAEAAERIVSKGSLTVN 327 Query 252 GISVHIS 258 \|+\|\| +\| Sbjct 328 GLSVGLS 334
Arabidopsis thaliana	Query 200 EDMTEDELREFFSQYGDVMDVFIPK-----PFRAF#AFVTFADDQIA-------QSLCGED 247 ++ + \|+\|\|++\| ++\| + \| +\|\|\| \| \|# \|\|\|\|\|++ +\| +\|\|++ Sbjct 193 QEASVDDLRDYFGRFGHIQDAYIPKDPKRSGHRGF#GFVTFAENGVADRVARRSHEICGQE 252 Query 248 LII 250 + \| Sbjct 253 VAI 255
Schistosoma japonicum	Query 203 TEDELREFFSQYGDVMDVFIPK-PF----RAF#AFVTFADDQIAQSLCGEDLIIKGISVH 256 \| \|+\|\| \|\|++\|+\| \|++\|\|+ \|+ \| \|#\|\|\| + \|+ \| \| \|+\|+ \| Sbjct 27 TIDDLRRVFSRFGEVGDIYIPRDPYTFESRGF#AFVRYCTDREA------DCAIRGMDGH 79
Arabidopsis thaliana	Query 201 DMTEDELREFFSQYGDVMDVFIPKP-----FRAF#AFVTFADDQIAQSLCGEDLIIKG 252 \| \|+ \|+\|+\|\| +\|+\|++ \| \| \| \|# \|\| \|\|\| +\|+ + \| \| \| Sbjct 16 DTNEERLKEYFSSFGEVIEAVILKDRTTGRARGF#GFVVFADPAVAEIVITEKHNIDG 72
Physcomitrella patens	Query 203 TEDELREFFSQYGDVMDVFI-----PKPFRAF#AFVTFADDQIA----QSLCGEDLIIKGI 253 \|+\| ++\| \|\| +\|+\| +\| \| \| \|# \|\|\|\|\| \|\| \| \|+\| \| \|\| + \| Sbjct 54 TDDNIKEAFSAFGEVTEVKIICDRDTGRSRGF#GFVTFATDQDAEAALQALDGRDLAGRTI 113 Query 254 SVHISN 259 \|+ + Sbjct 114 RVNYAT 119
Homo sapiens	Query 203 TEDELREFFSQYGDVMDVFIP-----KPFRAF#AFVTFADDQIAQ 241 + \| \|\| \| +\|\| \| \|\|+\|\| \| \| \|#\|\|\| \| \| \|\| Sbjct 26 SPDSLRRVFEKYGRVGDVYIPLEPHTKAPRGF#AFVRFHDRSDAQ 69
Aspergillus nidulans FGSC A4	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQS 242 + + \| +\|\|\|\|\|\|\|\|++ \|+ + +\| \| #\| +\|+\|\| \|++ Sbjct 336 DKLDEFTVREFFSQYGEITDLQL-QPHRKL#ALITYADHATAKA 377
Oryza sativa (japonica cultivar-group)	Query 200 EDMTEDELREFFSQYGDVMDVFI-----PKPFRAF#AFVTFADDQIAQSL 243 + +\|\|\|+ + \|\| +\|\| \|+\| \| \| \| \|# \|+ \|+ \|\|+ \| Sbjct 120 QALTEDDFKHFFQKYGPVVDHQIMRDHQTKRSRGF#GFIVFSSDQVVDDL 168
Brassica oleracea	Query 201 DMTEDELREFFSQYGDVMDVFIPKP-----FRAF#AFVTFADDQIAQSL 243 + \|\|\| \|\|++\| +\|+\|++ \| \| \| \|# \|+ \|\|\| +\|+ + Sbjct 16 ETTEDRLRDYFQSFGEVLEAVIMKDRSTGRARGF#GFLVFADPNVAERV 63
Theileria parva strain Muguga	Query 207 LREFFSQYGDVMDVFIPK-----PFRAF#AFVTFA---------DDQIAQSLCGEDLIIKG 252 \|\| +\|\|\|+\|+\|+\|\|+\|\|\| + \|# \|+\|\|\| \| + + \| ++\|+ Sbjct 354 LRTYFSQFGEVIDVYIPKDPHTQKGKGF#GFITFANKNSIHSALDPSLKHVVDGREIIVDY 413 Query 253 ISVH 256 \|\| Sbjct 414 ADVH 417
Nicotiana tabacum	Query 201 DMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQ 241 \|+\|++\|\|\|+ \|+\|+\|+\|+ \| \|\| + # \|\| \|+\| \|\| Sbjct 305 DVTDEELRQSFNQFGEVVSVKIPAG-KGC#GFVQFSDRSSAQ 344
Yarrowia lipolytica	Query 201 DMTEDELREFFSQYGDVMDVFI------PKPFRAF#AFVTFADDQIAQSLCGEDLII 250 \|+\|\|++ ++\|\| \|\|\| \|+\| + +\| \| \|# \|\|\|\| +\| ++ + \|+\| Sbjct 93 DVTEEDYKQFFEQYGTVIDAQLMIDKDSGRP-RGF#GFVTFDSEQAMNTILAQPLLI 147
Ashbya gossypii ATCC 10895	Query 201 DMTEDELREFFSQYGDVMDVFIPKP-----FRAF#AFVTFAD 236 + \|\|\| \|\|\|+\|\|+\|\|+\| +\| \| + \| \|# \|++\|\|\| Sbjct 173 ETTEDNLREYFSKYGNVTEVKIMRDGTTGRSRGF#GFLSFAD 213
Solanum tuberosum	Query 201 DMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQ 241 \|+\|++\|\|\|+ \|+\|+\|+\|+ \| \|\| + # \|\| \|+\| \|\| Sbjct 254 DVTDEELRQSFTQFGEVVSVKIPAG-KGC#GFVQFSDRSSAQ 293
Gallus gallus	Query 203 TEDELREFFSQYGDVMDVFIP-----KPFRAF#AFVTFADDQIAQ 241 + \| \|\| \| +\|\| \| \|\|+\|\| \| \| \|#\|\|\| \| \| + \|+ Sbjct 26 SPDTLRRVFEKYGRVGDVYIPRDRYTKESRGF#AFVRFHDKRDAE 69
Bombyx mori	Query 201 DMTEDELREFFSQYGDVMDVFIP-----KPFRAF#AFVTFADDQIAQSL 243 ++++\|\|+\| \|\|\|++\| +++\| +\| + \|# \|+\|\| +\|+ \| Sbjct 133 EISDDEIRNFFSEFGTILEVEMPFDKTKNQRKGF#CFITFESEQVVNDL 180
Strongylocentrotus purpuratus	Query 203 TEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCG--EDLIIKGISVHI 257 + \|\| \| \|\|+\|\| \| +\|++ + \|#\|\|\| \| \|++ \| \| +\| + \|+ \| + Sbjct 28 SRTELEEAFSRYGRVKNVWVARNPPGF#AFVMFEDERDASDACKALDDRNVCGVRVRV 84
Solanum tuberosum	Query 203 TEDELREFFSQYGDVMDVFI-----PKPFRAF#AFVTFADDQIA----QSLCGEDLIIKGI 253 \|++ \| \| \|\|\|\|\|+\|++ \| \| \|# \|\|\|\| \|+\| + + \|+\|\| + \| Sbjct 18 TDNTLSEAFSQYGEVVESKIINDRETGRSRGF#GFVTFKDEQAMRDAIEGMNGQDLDGRNI 77 Query 254 SVH 256 +\|+ Sbjct 78 TVN 80
Macaca mulatta	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPF-----RAF#AFVTFADDQIAQS---------LCG 245 +\| ++\|\| \| +\|\| ++\|\|++\| \| \| \|#\|+\| \| \| + \|+ +\|\| Sbjct 19 DDTRSEDLRREFGRYGPIVDVYVPLDFYTRRPRGF#AYVQFEDVRDAEDALHNLDRKWICG 78 Query 246 EDLIIK 251 + \|+ Sbjct 79 RQIEIQ 84
Medicago sativa	Query 205 DELREFFSQYGDVMDVFI-----PKPFRAF#AFVTFADDQ----IAQSLCGEDLIIKGISV 255 \| \| + \|\|\|\|\|+++\| \| \| \|# \|\|\|\|\|+++ + +++ \|+\|\| + \|+\| Sbjct 20 DALEKAFSQYGEIVDSKIINDRETGRSRGF#GFVTFANEKSMNDVIEAMNGQDLDGRNITV 79 Query 256 H 256 + Sbjct 80 N 80
Nitrococcus mobilis Nb-231	Query 203 TEDELREFFSQYGDVMDVFI------PKPFRAF#AFVTFADDQIA---QSLCGEDLIIKGI 253 +\|\|\|\|\|\| \|+ \|\|+\| \| + +\| \| \|# \|\|\| +\| \| ++\| \|+\| + + Sbjct 14 SEDELRELFAAYGNVDSVRLMTDRDTGRP-RGF#GFVTMSDTDAASAIEALDGKDFGGRNL 72 Query 254 SVH 256 \|+ Sbjct 73 RVN 75
Coxiella burnetii RSA 493	Query 201 DMTEDELREFFSQYGD------VMDVFIPKPFRAF#AFVTFADDQIAQSLC----GEDLII 250 \|+\| \|\|\|+ \|\| \|\|\|+ +\|\| + + \|#\|\|+\|+ \|\| \| \|\| Sbjct 14 DVTADELQSFFGQYGEIEEAKLIMDRETGRS-KGF#AFITYGTQDAAQEAVSKANGIDLQG 72 Query 251 KGISVHIS 258 + \| \|+\|+ Sbjct 73 RKIRVNIA 80
Ciona intestinalis	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHIS 258 \| \|\|+\|\|+\|++\| \|+\|\|\| \|\|+\|\| \|\|\|\|\|#\|\|\|\|\| \| +\| +\| \|\|\| +\| \|+\|\|+\|+ Sbjct 232 EKMTKDEIRQYFEQFGDVDDVYIPIPFRAF#AFVTFRDSNVAANLIGEDQVINGVSVYIN 290 Query 202 MTEDELREFFSQYGDVMDVFIPK----PFRAF#AFVTFADDQIAQSLCGEDLII 250 + \|\|+\|+\|+\|++\|\|+++ \| + + \| +# \|+ \|+ ++ + + + \|\| Sbjct 150 IAEDDLKEYFAKYGELVMVLVKRDENGKSRGY#GFIRFSSYEVQELVLSQRHII 202
Drosophila pseudoobscura	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHISN 259 \|\|+ \|+\|\|\|+\|\|++\|+\| \|\|\|\|\|+\|\|\|\|\|#+\|\|\|\| \| +\|\|\|\|\|\|\|\| \|\|\|\|+\|\|\|+\|\| Sbjct 201 EDINSDDLREYFSKFGEVTDVFIPRPFRAF#SFVTFFDPDVAQSLCGEDHIIKGVSVHVSN 260 Query 203 TEDELREFFSQYGDVMDVFIPKPFRA-----F#AFVTFA 235 \|\|\| \|\|\|+\| \|\|+\|+ \| \| ++ \|# \|\| \| Sbjct 119 TEDSLREYFESYGEVLMAQIKKDVKSGQSKGF#GFVRFG 156
Xenopus tropicalis	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHIS 258 \|\|\|+ +\|\|\|+\|\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|+\| Sbjct 200 EDMSAEELRQFFSQYGEVVDVFIPKPFRAF#AFVTFADDQVAQSLCGEDLIIKGVSVHVS 258 Query 203 TEDELREFFSQYGDVMDVFIPKPF-----RAF#AFVTFADDQIAQSLCGEDLIIKG 252 \|\| +\|+++\|\| +\|+\|+ \| + \| + \|# \|\| \|\|\| + + + +\| \| Sbjct 117 TEQDLKDYFSTFGEVIMVQVKKDAKTGHSKGF#GFVRFADYETQVKVMSQRHMIDG 171
Brassica oleracea	Query 201 DMTEDELREFFSQYGDVMDVFIPKP-----FRAF#AFVTFADDQIAQSL 243 + \|\|\| \|\|\|+\| +\|+\|++ \| \| \| \|# \|+ \|\|\| +\|+ + Sbjct 15 ETTEDRLREYFQSFGEVLEAVIMKDRATGRARGF#GFLVFADPTVAERV 62 Query 202 MTEDELREFFSQYGDVMDVFI------PKPFRAF#AFVTFADDQ 238 +\|\| \| +++\|+\|+\| + \|\| + +\| \| \|# \|++\| ++ Sbjct 116 VTEAEFKKYFAQFGTITDVVVMYDHRTQRP-RGF#GFISFESEE 157
Oryza sativa Japonica Group	Query 200 EDMTEDELREFFSQYGDVMDVFIPKP-----FRAF#AFVTFADDQIAQSLCGEDLIIKGIS 254 \| \|\|\| \|\| +\| ++\| ++\| +\|\|\| \| \|# \|\|\|\|\|\|+ +\| + \| \| Sbjct 400 EANTED-LRHYFGKFGRIVDAYIPKDPKRSGHRGF#GFVTFADEGVADRVARRSHEILGHE 458 Query 255 VHI 257 \| \| Sbjct 459 VAI 461 Query 200 EDMTEDELREFFSQYGDVMDVFIPKP-----FRAF#AFVTFADDQIAQSLCGEDLIIKGIS 254 + + \| \| \| \|\|++ \|+++\|\| \| # \|+\|\| + \|+ + + \| + Sbjct 261 QSVDESMFRRHFEAYGEITDLYMPKEHGSKGHRGI#GFITFQSAESVDSIMQDSHELDGTT 320 Query 255 V 255 \| Query 255 V 255
Canis familiaris	Query 200 EDMTEDELREFFSQYGDVMDVFIPKPFRAF#AFVTFADDQIAQSLCGEDLIIKGISVHISN 259 \|\|\|\| \|\|\|\|+\|\| \|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 323 EDMTADELRQFFCQYGEVVDVFIPKPFRAF#AFVTFADDQVAQSLCGEDLIIKGISVHISN 382 Query 203 TEDELREFFSQYGDVMDVFIPKPF-----RAF#AFVTFADDQIAQSLCGEDLIIKG 252 \|\| +\|+\|+\|\| +\|+\|+ \| + \| + \|# \|\| \| + + + + +\| \| Sbjct 239 TEQDLKEYFSTFGEVLMVQVKKDIKTGHSKGF#GFVRFTEYETQVKVMSQRHMIDG 293

[Site 2] FADDQIAQSL²⁴³-CGEDLIIKGI

Leu²⁴³ Cys

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Phe²³⁴	Ala²³⁵	Asp²³⁶	Asp²³⁷	Gln²³⁸	Ile²³⁹	Ala²⁴⁰	Gln²⁴¹	Ser²⁴²	Leu²⁴³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Cys²⁴⁴	Gly²⁴⁵	Glu²⁴⁶	Asp²⁴⁷	Leu²⁴⁸	Ile²⁴⁹	Ile²⁵⁰	Lys²⁵¹	Gly²⁵²	Ile²⁵³

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
YGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERS

#	organism	max score	hits	top seq
1	Homo sapiens	131.00	1	TAR DNA binding protein
2	Canis familiaris	127.00	1	PREDICTED: similar to TAR DNA binding protein iso
3	Bos taurus	125.00	1	TAR DNA binding protein
4	Mus musculus	124.00	8	unnamed protein product
5	Rattus norvegicus	123.00	1	TAR DNA binding protein
6	Xenopus tropicalis	119.00	2	TADBP_XENTR TAR DNA-binding protein 43 (TDP-43) g
7	Xenopus laevis	118.00	2	mitotic phosphoprotein 39
8	N/A	115.00	2	A Chain A, Solution Structure Of Rrm Domain In Ta
9	Tetraodon nigroviridis	112.00	2	unnamed protein product
10	Danio rerio	112.00	1	TAR DNA binding protein
11	Danio rerio	106.00	1	TAR DNA binding protein, like
12	Drosophila melanogaster	90.50	3	TAR-binding protein
13	Drosophila melanogaster	90.50	2	TBPH CG10327-PA, isoform A
14	Drosophila pseudoobscura	88.20	1	GA10247-PA
15	Ciona intestinalis	71.60	1	Tardbp protein
16	Schistosoma japonicum	65.90	2	SJCHGC08328 protein
17	Caenorhabditis briggsae AF16	51.20	1	Hypothetical protein CBG03235
18	Caenorhabditis elegans	51.20	1	Temporarily Assigned Gene name family member (tag
19	Oryza sativa (japonica cultivar-group)	35.00	2	Os08g0492100
20	Arabidopsis thaliana	33.10	4	RNA-binding like protein
21	Arabidopsis thaliana	32.30	13	RNA recognition motif (RRM)-containing protein
22	Entamoeba histolytica HM-1:IMSS	32.30	1	RNA recognition motif domain containing protein
23	Oryza sativa Japonica Group	31.60	1	putative RNA-binding like protein
24	Brassica oleracea	31.20	1	hypothetical protein 25.t00002
25	Aspergillus nidulans FGSC A4	30.40	1	hypothetical protein AN6256.2
26	Aspergillus oryzae RIB40	30.40	1	hypothetical protein
27	Physcomitrella patens	30.40	1	glycine-rich RNA-binding protein

organism	matching
Homo sapiens	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNRQLERS 273 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNRQLERS 273
Canis familiaris	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNRQLERS 273 \|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 337 YGEVVDVFIPKPFRAFAFVTFADDQVAQSL#CGEDLIIKGISVHISNAEPKHNSNRQLERS 396
Bos taurus	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNRQLERS 273 \|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 214 YGEVVDVFIPKPFRAFAFVTFADDQVAQSL#CGEDLIIKGISVHISNAEPKHNSNRQLERS 273
Mus musculus	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNRQLERS 273 \|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|++\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 214 YGEVVDVFIPKPFRAFAFVTFADDKVAQSL#CGEDLIIKGISVHISNAEPKHNSNRQLERS 273
Rattus norvegicus	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNRQLERS 273 \|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|++\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 214 YGEVVDVFIPKPFRAFAFVTFADDKVAQSL#CGEDLIIKGISVHISNAEPKHNSNRQLERS 273
Xenopus tropicalis	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNRQLER 272 \|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|#\|\|\|\|\|\|\|\|\|+\|\|\|+\| \|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 214 YGEVVDVFIPKPFRAFAFVTFADDQVAQSL#CGEDLIIKGVSVHVSTAEPKHNNNRQLER 272
Xenopus laevis	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNRQLER 272 \|\|+\|+\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+\|\|\|\|#\|\|\|\|\|\|\|\|\|+\|\|\|+\| \|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 213 YGEVVDVFIPKPFRAFSFVTFADDQVAQSL#CGEDLIIKGVSVHVSTAEPKHNNNRQLER 271
N/A	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSN 267 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSN 267
Tetraodon nigroviridis	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNRQLERS 273 \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|#\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\| \|\|\| +\|+ Sbjct 284 YGEVTDVFIPKPFRAFAFVTFADDQVAQSL#CGEDLIIKGVSVHISNAEPKH-GNRQYDRT 342
Danio rerio	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNRQ-LER 272 \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\| +\|#\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|+ \|\| +\|\| Sbjct 220 YGEVTDVFIPKPFRAFAFVTFADDQVAAAL#CGEDLIIKGVSVHISNAEPKHNNTRQMMER 279 Query 273 S 273 + Sbjct 280 A 280
Danio rerio	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNRQL 270 \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|#\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|+ \| Sbjct 220 YGEVTDVFIPKPFRAFAFVTFADDQVAQSL#CGEDLIIKGTSVHISNAEPKHNNIHHL 276
Drosophila melanogaster	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNR 268 +\|+\| \|\|\|\|\|+\|\|\|\|\|+\|\|\|\| \| +\|\|\|\|#\|\|\|\| \|\|\|\|+\|\|\|+\|\|\| \|\| \|\| Sbjct 215 FGEVTDVFIPRPFRAFSFVTFLDPDVAQSL#CGEDHIIKGVSVHVSNAAPKAEQNR 269
Drosophila melanogaster	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNR 268 +\|+\| \|\|\|\|\|+\|\|\|\|\|+\|\|\|\| \| +\|\|\|\|#\|\|\|\| \|\|\|\|+\|\|\|+\|\|\| \|\| \|\| Sbjct 215 FGEVTDVFIPRPFRAFSFVTFLDPDVAQSL#CGEDHIIKGVSVHVSNAAPKAEQNR 269
Drosophila pseudoobscura	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNR 268 +\|+\| \|\|\|\|\|+\|\|\|\|\|+\|\|\|\| \| +\|\|\|\|#\|\|\|\| \|\|\|\|+\|\|\|+\|\|\| \|\| +\| Sbjct 215 FGEVTDVFIPRPFRAFSFVTFFDPDVAQSL#CGEDHIIKGVSVHVSNAAPKAEQSR 269
Ciona intestinalis	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPK 263 +\|\|\| \|\|+\|\| \|\|\|\|\|\|\|\|\|\| \| +\| +\|# \|\|\| +\| \|+\|\|+\|+ \|+\|\| Sbjct 246 FGDVDDVYIPIPFRAFAFVTFRDSNVAANL#IGEDQVINGVSVYINTADPK 295
Schistosoma japonicum	Query 214 YGDVMDVFIPKPFRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPK 263 +\| \|+\|\|\|\|\|\|\|\|\|+\|\|\|\|+\| \| \|+\| \|# \|+\| +\|+\|+++ \| +\| \|\| Sbjct 112 FGRVIDVFIPKPFRSFAFVSFEDPQVATDL#LGKDQVIEGVTLSIGSAVPK 161
Caenorhabditis briggsae AF16	Query 217 VMDVFIPKPFRAFAFVTFADDQIAQSL#CGE-DLIIKGISVHISNAEPKHNS 266 \| \|\|\|\|\|\|\|\|\| \|\|\|\|\| + + \|+\|+# + \| + \|+\|\| +\| \|+\|+ + Sbjct 292 VTDVFIPKPFRGFAFVTLSSAEAAESI#VSKGSLTVNGLSVGLSIAQPREEN 342
Caenorhabditis elegans	Query 217 VMDVFIPKPFRAFAFVTFADDQIAQSL#CGE-DLIIKGISVHISNAEPKHNSNRQL 270 \| \|\|\|\|\|\|\|\|\| \|\|\|\|\| + + \|+ +# + \| + \|+\|\| +\| \|+\|+ +\|+ + Sbjct 292 VTDVFIPKPFRGFAFVTLSSAEAAERI#VSKGSLTVNGLSVGLSIAQPREENNQSV 346
Oryza sativa (japonica cultivar-group)	Query 214 YGDVMDVFI-----PKPFRAFAFVTFADDQIAQSL#C--GEDLIIKGISVHISNAEPKHNS 266 \|\| \|+\| \| \| \| \| \|+ \|+ \|\|+ \|# \| + + \| \| \| \|\|\|\| +\| Sbjct 134 YGPVVDHQIMRDHQTKRSRGFGFIVFSSDQVVDDL#LANGNMIDLAGAKVEIKKAEPKKSS 193 Query 267 N 267 \| Query 267 N 267
Arabidopsis thaliana	Query 214 YGDVMDVFIPKP-----FRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEP 262 +\| + \| +\|\|\| \| \| \|\|\|\|\|++ +\| +# \| \| \| \| +\| \| Sbjct 207 FGHIQDAYIPKDPKRSGHRGFGFVTFAENGVADRV#ARRSHEICGQEVAIDSATP 260
Arabidopsis thaliana	Query 214 YGDVMDVFIPKP-----FRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEP 262 +\| + \| +\|\|\| \| \| \|\|\|\|\|++ +\| +# \| \| \| \| +\| \| Sbjct 263 FGHIQDAYIPKDPKRSGHRGFGFVTFAENGVADRV#ARRSHEICGQEVAIDSATP 316 Query 214 YGDVMDVFIPKPF-----RAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSN 267 \|\|++ \|+++\|\| + \| \|+\|\|+ + \|# + + \| +\| + \| \|\| + + Sbjct 114 YGEITDLYMPKDYNSKQHRGIGFITFSSADSVEDL#MEDTHDLGGTTVAVDRATPKEDDH 172
Entamoeba histolytica HM-1:IMSS	Query 214 YGDVMDVFIP-----KPFRAFAFVTFADDQIAQSL#CGE--DLIIKGISVHISNAEPKHNS 266 +\|++ ++ +\| \| + \| \|\| \| \| \| #\| \| + \|\|\| \|\|++ \|+ \| Sbjct 210 FGNIAEIHLPIDKITKKSKGFGFVLFVVPQDAVKA#CNEMDNKFIKGRIVHVTYAKADPYS 269 Query 267 NRQLERS 273 \|+\|+ \| Sbjct 270 NQQVGES 276
Oryza sativa Japonica Group	Query 214 YGDVMDVFIPKP-----FRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEP 262 +\| ++\| +\|\|\| \| \| \|\|\|\|\|\|+ +\| +# \| \| \| \| \| \| Sbjct 413 FGRIVDAYIPKDPKRSGHRGFGFVTFADEGVADRV#ARRSHEILGHEVAIDTAAP 466 Query 214 YGDVMDVFIPKPF-----RAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPKHNSNR 268 \|\|++ \|+++\|\| \| \|+\|\| + \|+# + + \| +\| + \| \|\| \| Sbjct 275 YGEITDLYMPKEHGSKGHRGIGFITFQSAESVDSI#MQDSHELDGTTVVVDRATPKDEEVR 334
Brassica oleracea	Query 214 YGDVMDVFIPKP-----FRAFAFVTFADDQIAQSL#CGEDLIIKGISVHISNAEPK--HNS 266 +\|+\|++ \| \| \| \| \|+ \|\|\| +\|+ +# +\| \| \| \| \|+ \| \| Sbjct 29 FGEVLEAVIMKDRSTGRARGFGFLVFADPNVAERV#VLLRHVIDGKLVEAKKAVPRDDHKS 88 Query 267 NRQLERS 273 \| \|+ \| Sbjct 89 NSSLQGS 95
Aspergillus nidulans FGSC A4	Query 214 YGDVMDVFIPKP--------FRAFAFVTFADDQIAQ----SL#CGEDLIIKGISVHISNAE 261 +\|+\|+\|+ +\|\|\| \| \| +\| \| + \|+ ++# \| +\| \| ++ ++ \|+ Sbjct 32 FGEVVDITLPKPDLPNSNDNHRGFGYVEFDSPEDAKEAIDNM#DGSELY--GRTIKVAPAK 89 Query 262 PKHNSNRQL 270 \|+ ++\| \| Sbjct 90 PQKDANEGL 98
Aspergillus oryzae RIB40	Query 214 YGDVMDVFIPKP--------FRAFAFVTFADDQIAQ----SL#CGEDLIIKGISVHISNAE 261 +\|+\|+\|+ +\|\|\| \| \| +\| \| + \|+ ++# \| +\| \| ++ ++ \|+ Sbjct 32 FGEVVDITLPKPDVPNSNELHRGFGYVEFEVPEDAKEAIDNM#DGSELY--GRTIKVAAAK 89 Query 262 PKHNSNRQL 270 \|+ +\|\| \| Sbjct 90 PQKDSNEGL 98
Physcomitrella patens	Query 214 YGDVMDVFI-----PKPFRAFAFVTFADDQIA----QSL#CGEDLIIKGISVHISNAEPKH 264 +\|+\| +\| \| \| \| \|\|\|\|\| \|\| \| \|+\|# \| \|\| \| ++ ++ \| + Sbjct 65 FGEVTEVKIICDRDTGRSRGFGFVTFATDQDAEAALQAL#DGRDL--AGRTIRVNYATKQS 122 Query 265 NSNRQ 269 +\|\| Sbjct 123 PQDRQ 127

[Site 3] GGNPGGFGNQ²⁸⁶-GGFGNSRGGG

Gln²⁸⁶ Gly

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Gly²⁷⁷	Gly²⁷⁸	Asn²⁷⁹	Pro²⁸⁰	Gly²⁸¹	Gly²⁸²	Phe²⁸³	Gly²⁸⁴	Asn²⁸⁵	Gln²⁸⁶

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gly²⁸⁷	Gly²⁸⁸	Phe²⁸⁹	Gly²⁹⁰	Asn²⁹¹	Ser²⁹²	Arg²⁹³	Gly²⁹⁴	Gly²⁹⁵	Gly²⁹⁶

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
ISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAF

#	organism	max score	hits	top seq
1	Homo sapiens	116.00	1	TAR DNA binding protein
2	Mus musculus	113.00	8	unnamed protein product
3	Canis familiaris	55.50	1	PREDICTED: similar to TAR DNA binding protein iso
4	Bos taurus	54.30	1	TAR DNA binding protein
5	Rattus norvegicus	49.30	1	TAR DNA binding protein
6	Xenopus tropicalis	46.60	2	TADBP_XENTR TAR DNA-binding protein 43 (TDP-43) g
7	Xenopus laevis	45.10	1	mitotic phosphoprotein 39

organism	matching
Homo sapiens	Query 257 ISNAEPKHNSNRQLERSGRFGGNPGGFGNQ#GGFGNSRGGGAGLGNNQGSNMGGGMNFGAF 316 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 257 ISNAEPKHNSNRQLERSGRFGGNPGGFGNQ#GGFGNSRGGGAGLGNNQGSNMGGGMNFGAF 316
Mus musculus	Query 257 ISNAEPKHNSNRQLERSGRFGGNPGGFGNQ#GGFGNSRGGGAGLGNNQGSNMGGGMNFGAF 316 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\| Sbjct 257 ISNAEPKHNSNRQLERSGRFGGNPGGFGNQ#GGFGNSRGGGAGLGNNQGGNMGGGMNFGAF 316
Canis familiaris	Query 257 ISNAEPKHNSNRQLERSGRFGGNPG 281 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 257 ISNAEPKHNSNRQLERSGRFGGNPG 281
Bos taurus	Query 257 ISNAEPKHNSNRQLERSGRFGGNP 280 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 257 ISNAEPKHNSNRQLERSGRFGGNP 280
Rattus norvegicus	Query 257 ISNAEPKHNSNRQLERSGRFGGN 279 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 257 ISNAEPKHNSNRQLERSGRFGGK 279
Xenopus tropicalis	Query 257 ISNAEPKHNSNRQLERSGRFGGNPGGFGNQ#GGFGNSRGGGAGL 299 +\| \|\|\|\|\|\|+\|\|\|\|\|\| \|\|\| \| \|\|\|\|# \|+ \|\|\| \| Sbjct 257 VSTAEPKHNNNRQLERGGRFPG--PSFGNQ#-GYPNSRPSSGAL 296
Xenopus laevis	Query 257 ISNAEPKHNSNRQLERSGRFGGNPGGFGNQ#GGFGNSRGGGA 297 +\| \|\|\|\|\|\|+\|\|\|\|\|\| \|\|\| \| \|\|+\|#\| \| \|\| Sbjct 256 VSTAEPKHNNNRQLEREGRFPG--PSFGSQ#GYPSNRPSGGT 294

[Site 4] QGGFGNSRGG²⁹⁵-GAGLGNNQGS

Gly²⁹⁵ Gly

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Gln²⁸⁶	Gly²⁸⁷	Gly²⁸⁸	Phe²⁸⁹	Gly²⁹⁰	Asn²⁹¹	Ser²⁹²	Arg²⁹³	Gly²⁹⁴	Gly²⁹⁵

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gly²⁹⁶	Ala²⁹⁷	Gly²⁹⁸	Leu²⁹⁹	Gly³⁰⁰	Asn³⁰¹	Asn³⁰²	Gln³⁰³	Gly³⁰⁴	Ser³⁰⁵

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
SNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAA

#	organism	max score	hits	top seq
1	Homo sapiens	114.00	1	TAR DNA binding protein
2	Mus musculus	112.00	3	TAR DNA binding protein isoform 1
3	Xenopus tropicalis	32.30	1	TADBP_XENTR TAR DNA-binding protein 43 (TDP-43) g

organism	matching
Homo sapiens	Query 266 SNRQLERSGRFGGNPGGFGNQGGFGNSRGG#GAGLGNNQGSNMGGGMNFGAFSINPAMMAA 325 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 266 SNRQLERSGRFGGNPGGFGNQGGFGNSRGG#GAGLGNNQGSNMGGGMNFGAFSINPAMMAA 325
Mus musculus	Query 266 SNRQLERSGRFGGNPGGFGNQGGFGNSRGG#GAGLGNNQGSNMGGGMNFGAFSINPAMMAA 325 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 266 SNRQLERSGRFGGNPGGFGNQGGFGNSRGG#GAGLGNNQGGNMGGGMNFGAFSINPAMMAA 325
Xenopus tropicalis	Query 267 NRQLERSGRFGGNPGGFGNQGGFGNSRGG#GAGLGNNQGSNMGGGMNFG--AFSINP 320 \|\|\|\|\|\| \|\|\| \| \|\|\|\|\| + \|\|\| # \|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\|\| Sbjct 267 NRQLERGGRFPG--PSFGNQG-YPNSRPS#SGALGNNQGGNMGGGGGMNFGAFSINP 319

[Site 5] GFGNSRGGGA²⁹⁷-GLGNNQGSNM

Ala²⁹⁷ Gly

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Gly²⁸⁸	Phe²⁸⁹	Gly²⁹⁰	Asn²⁹¹	Ser²⁹²	Arg²⁹³	Gly²⁹⁴	Gly²⁹⁵	Gly²⁹⁶	Ala²⁹⁷

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gly²⁹⁸	Leu²⁹⁹	Gly³⁰⁰	Asn³⁰¹	Asn³⁰²	Gln³⁰³	Gly³⁰⁴	Ser³⁰⁵	Asn³⁰⁶	Met³⁰⁷

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
RQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQ

#	organism	max score	hits	top seq
1	Homo sapiens	115.00	1	TAR DNA binding protein
2	Mus musculus	112.00	3	TAR DNA binding protein isoform 1
3	Xenopus tropicalis	30.40	1	hypothetical protein LOC394651

organism	matching
Homo sapiens	Query 268 RQLERSGRFGGNPGGFGNQGGFGNSRGGGA#GLGNNQGSNMGGGMNFGAFSINPAMMAAAQ 327 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 268 RQLERSGRFGGNPGGFGNQGGFGNSRGGGA#GLGNNQGSNMGGGMNFGAFSINPAMMAAAQ 327
Mus musculus	Query 268 RQLERSGRFGGNPGGFGNQGGFGNSRGGGA#GLGNNQGSNMGGGMNFGAFSINPAMMAAAQ 327 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 268 RQLERSGRFGGNPGGFGNQGGFGNSRGGGA#GLGNNQGGNMGGGMNFGAFSINPAMMAAAQ 327
Xenopus tropicalis	Query 268 RQLERSGRFGGNPGGFGNQGGFGNSRGGGA#GLGNNQGSNMGGGMNFG--AFSINP 320 \|\|\|\|\| \|\|\| \| \|\|\|\|\| + \|\|\| # \|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\|\| Sbjct 266 RQLERGGRFPG--PSFGNQG-YPNSRPSSG#ALGNNQGGNMGGGGGMNFGAFSINP 317

[Site 6] RGGGAGLGNN³⁰²-QGSNMGGGMN

Asn³⁰² Gln

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Arg²⁹³	Gly²⁹⁴	Gly²⁹⁵	Gly²⁹⁶	Ala²⁹⁷	Gly²⁹⁸	Leu²⁹⁹	Gly³⁰⁰	Asn³⁰¹	Asn³⁰²

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gln³⁰³	Gly³⁰⁴	Ser³⁰⁵	Asn³⁰⁶	Met³⁰⁷	Gly³⁰⁸	Gly³⁰⁹	Gly³¹⁰	Met³¹¹	Asn³¹²

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
SGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQS

#	organism	max score	hits	top seq
1	Homo sapiens	113.00	1	TAR DNA binding protein
2	Mus musculus	110.00	2	unnamed protein product

organism	matching
Homo sapiens	Query 273 SGRFGGNPGGFGNQGGFGNSRGGGAGLGNN#QGSNMGGGMNFGAFSINPAMMAAAQAALQS 332 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 273 SGRFGGNPGGFGNQGGFGNSRGGGAGLGNN#QGSNMGGGMNFGAFSINPAMMAAAQAALQS 332
Mus musculus	Query 273 SGRFGGNPGGFGNQGGFGNSRGGGAGLGNN#QGSNMGGGMNFGAFSINPAMMAAAQAALQS 332 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 273 SGRFGGNPGGFGNQGGFGNSRGGGAGLGNN#QGGNMGGGMNFGAFSINPAMMAAAQAALQS 332

[Site 7] GGGAGLGNNQ³⁰³-GSNMGGGMNF

Gln³⁰³ Gly

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Gly²⁹⁴	Gly²⁹⁵	Gly²⁹⁶	Ala²⁹⁷	Gly²⁹⁸	Leu²⁹⁹	Gly³⁰⁰	Asn³⁰¹	Asn³⁰²	Gln³⁰³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gly³⁰⁴	Ser³⁰⁵	Asn³⁰⁶	Met³⁰⁷	Gly³⁰⁸	Gly³⁰⁹	Gly³¹⁰	Met³¹¹	Asn³¹²	Phe³¹³

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
GRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSS

#	organism	max score	hits	top seq
1	Homo sapiens	113.00	1	TAR DNA binding protein
2	Mus musculus	110.00	2	unnamed protein product

organism	matching
Homo sapiens	Query 274 GRFGGNPGGFGNQGGFGNSRGGGAGLGNNQ#GSNMGGGMNFGAFSINPAMMAAAQAALQSS 333 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 274 GRFGGNPGGFGNQGGFGNSRGGGAGLGNNQ#GSNMGGGMNFGAFSINPAMMAAAQAALQSS 333
Mus musculus	Query 274 GRFGGNPGGFGNQGGFGNSRGGGAGLGNNQ#GSNMGGGMNFGAFSINPAMMAAAQAALQSS 333 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 274 GRFGGNPGGFGNQGGFGNSRGGGAGLGNNQ#GGNMGGGMNFGAFSINPAMMAAAQAALQSS 333

[Site 8] GGAGLGNNQG³⁰⁴-SNMGGGMNFG

Gly³⁰⁴ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Gly²⁹⁵	Gly²⁹⁶	Ala²⁹⁷	Gly²⁹⁸	Leu²⁹⁹	Gly³⁰⁰	Asn³⁰¹	Asn³⁰²	Gln³⁰³	Gly³⁰⁴

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser³⁰⁵	Asn³⁰⁶	Met³⁰⁷	Gly³⁰⁸	Gly³⁰⁹	Gly³¹⁰	Met³¹¹	Asn³¹²	Phe³¹³	Gly³¹⁴

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
RFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSW

#	organism	max score	hits	top seq
1	Homo sapiens	115.00	1	TAR DNA binding protein
2	Mus musculus	112.00	2	unnamed protein product

organism	matching
Homo sapiens	Query 275 RFGGNPGGFGNQGGFGNSRGGGAGLGNNQG#SNMGGGMNFGAFSINPAMMAAAQAALQSSW 334 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 275 RFGGNPGGFGNQGGFGNSRGGGAGLGNNQG#SNMGGGMNFGAFSINPAMMAAAQAALQSSW 334
Mus musculus	Query 275 RFGGNPGGFGNQGGFGNSRGGGAGLGNNQG#SNMGGGMNFGAFSINPAMMAAAQAALQSSW 334 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|# \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 275 RFGGNPGGFGNQGGFGNSRGGGAGLGNNQG#GNMGGGMNFGAFSINPAMMAAAQAALQSSW 334

[Site 9] AFSINPAMMA³²⁴-AAQAALQSSW

Ala³²⁴ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ala³¹⁵	Phe³¹⁶	Ser³¹⁷	Ile³¹⁸	Asn³¹⁹	Pro³²⁰	Ala³²¹	Met³²²	Met³²³	Ala³²⁴

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala³²⁵	Ala³²⁶	Gln³²⁷	Ala³²⁸	Ala³²⁹	Leu³³⁰	Gln³³¹	Ser³³²	Ser³³³	Trp³³⁴

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
GGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQ

#	organism	max score	hits	top seq
1	Homo sapiens	115.00	1	TAR DNA binding protein
2	Mus musculus	112.00	3	TAR DNA binding protein isoform 1
3	Xenopus tropicalis	59.30	2	TADBP_XENTR TAR DNA-binding protein 43 (TDP-43) g
4	Xenopus laevis	57.40	1	mitotic phosphoprotein 39
5	Canis familiaris	56.60	1	PREDICTED: similar to glyceraldehyde-3-phosphate
6	Tetraodon nigroviridis	48.10	1	unnamed protein product
7	Danio rerio	37.40	1	TAR DNA binding protein

organism	matching
Homo sapiens	Query 295 GGAGLGNNQGSNMGGGMNFGAFSINPAMMA#AAQAALQSSWGMMGMLASQQNQSGPSGNNQ 354 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 295 GGAGLGNNQGSNMGGGMNFGAFSINPAMMA#AAQAALQSSWGMMGMLASQQNQSGPSGNNQ 354
Mus musculus	Query 295 GGAGLGNNQGSNMGGGMNFGAFSINPAMMA#AAQAALQSSWGMMGMLASQQNQSGPSGNNQ 354 \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 295 GGAGLGNNQGGNMGGGMNFGAFSINPAMMA#AAQAALQSSWGMMGMLASQQNQSGPSGNNQ 354
Xenopus tropicalis	Query 315 AFSINPAMMA#AAQAALQSSWGMMGMLASQQNQSGPSGNNQ 354 \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|+\|\| Sbjct 314 AFSINPAMMA#AAQAALQSSWGMMGMLASQQNQSGPQGSNQ 353
Xenopus laevis	Query 315 AFSINPAMMA#AAQAALQSSWGMMGMLASQQNQSGPSGNN 353 \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|+\| Sbjct 317 AFSINPAMMA#AAQAALQSSWGMMGMLASQQNQSGPQGSN 355
Canis familiaris	Query 322 MMA#AAQAALQSSWGMMGMLASQQNQSGPS 350 \| #\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MTV#AAQAALQSSWGMMGMLASQQNQSGPS 29
Tetraodon nigroviridis	Query 312 NFGAFSINPAMMA#AAQAALQSSWGMMGMLASQQ 344 \|\|\|+\|\|+\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 369 NFGSFSLNPAMMA#AAQAALQSSWGMMGMLASQQ 401
Danio rerio	Query 316 FSINPAMMA#AAQAALQSSWGMMGMLASQQNQ 346 \|++\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 316 FNLNPAMMA#AAQAALQSSWGMMGMLA-QQNQ 345

[Site 10] MGMLASQQNQ³⁴⁶-SGPSGNNQNQ

Gln³⁴⁶ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Met³³⁷	Gly³³⁸	Met³³⁹	Leu³⁴⁰	Ala³⁴¹	Ser³⁴²	Gln³⁴³	Gln³⁴⁴	Asn³⁴⁵	Gln³⁴⁶

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser³⁴⁷	Gly³⁴⁸	Pro³⁴⁹	Ser³⁵⁰	Gly³⁵¹	Asn³⁵²	Asn³⁵³	Gln³⁵⁴	Asn³⁵⁵	Gln³⁵⁶

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
SINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSG

#	organism	max score	hits	top seq
1	Homo sapiens	118.00	1	TAR DNA binding protein
2	Mus musculus	114.00	3	TAR DNA binding protein isoform 1
3	Xenopus tropicalis	73.60	2	TADBP_XENTR TAR DNA-binding protein 43 (TDP-43) g
4	Xenopus laevis	72.40	1	mitotic phosphoprotein 39
5	Canis familiaris	57.00	1	PREDICTED: similar to glyceraldehyde-3-phosphate
6	Tetraodon nigroviridis	46.20	1	unnamed protein product
7	Danio rerio	40.40	1	TAR DNA binding protein

organism	matching
Homo sapiens	Query 317 SINPAMMAAAQAALQSSWGMMGMLASQQNQ#SGPSGNNQNQGNMQREPNQAFGSGNNSYSG 376 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 317 SINPAMMAAAQAALQSSWGMMGMLASQQNQ#SGPSGNNQNQGNMQREPNQAFGSGNNSYSG 376
Mus musculus	Query 317 SINPAMMAAAQAALQSSWGMMGMLASQQNQ#SGPSGNNQNQGNMQREPNQAFGSGNNSYSG 376 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|+\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 317 SINPAMMAAAQAALQSSWGMMGMLASQQNQ#SGPSGNNQSQGSMQREPNQAFGSGNNSYSG 376
Xenopus tropicalis	Query 317 SINPAMMAAAQAALQSSWGMMGMLASQQNQ#SGPSGNNQNQGNMQREPNQAFGSGNNSY 374 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\| \|+\|\| \|\|\| \|\|+ \|+\|\|\| \|\|\|\| Sbjct 316 SINPAMMAAAQAALQSSWGMMGMLASQQNQ#SGPQGSNQGQGNQQRDQPQSFGS-NNSY 372
Xenopus laevis	Query 317 SINPAMMAAAQAALQSSWGMMGMLASQQNQ#SGPSGNNQNQGNMQREPNQAFGSGNNSY 374 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\| \|+\| \|\|\| \|\|\| \|+\|\|\| \|\|\|\| Sbjct 319 SINPAMMAAAQAALQSSWGMMGMLASQQNQ#SGPQGSNPGQGNQQREQPQSFGS-NNSY 375
Canis familiaris	Query 322 MMAAAQAALQSSWGMMGMLASQQNQ#SGPS 350 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\| Sbjct 1 MTVAAQAALQSSWGMMGMLASQQNQ#SGPS 29
Tetraodon nigroviridis	Query 317 SINPAMMAAAQAALQSSWGMMGMLASQQNQ#SGPSGNNQNQGNMQREPNQAFGSGNNSY 374 \|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| #+ \|\|+ + + \|+ +\|+\|\|+\|\|++\| Sbjct 374 SLNPAMMAAAQAALQSSWGMMGMLASQQTS#N--SGSTSSGTSSSRDQSQSFGTGNSNY 429
Danio rerio	Query 318 INPAMMAAAQAALQSSWGMMGMLASQQNQ#SGPSGNNQNQGNMQREPNQAFGSGNNSY 374 +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|#\|\| \|\| + + + \|+ \| + \| \|++\| Sbjct 318 LNPAMMAAAQAALQSSWGMMGMLA-QQNQ#SGTSGTSTSGTSSSRDQAQTYSSANSNY 373

* References

[PubMed ID: 24440855] De Marco G, Lomartire A, Mandili G, Lupino E, Buccinna B, Ramondetti C, Moglia C, Novelli F, Piccinini M, Mostert M, Rinaudo MT, Chio A, Calvo A, Reduced cellular Ca(2+) availability enhances TDP-43 cleavage by apoptotic caspases. Biochim Biophys Acta. 2014 Apr;1843(4):725-34. doi: 10.1016/j.bbamcr.2014.01.010.

» More (11) « Hide Below

[PubMed ID: 24440310] ... Mompean M, Buratti E, Guarnaccia C, Brito RM, Chakrabartty A, Baralle FE, Laurents DV, "Structural characterization of the minimal segment of TDP-43 competent for aggregation". Arch Biochem Biophys. 2014 Mar 1;545:53-62. doi: 10.1016/j.abb.2014.01.007. Epub

[PubMed ID: 24507191] ... Alami NH, Smith RB, Carrasco MA, Williams LA, Winborn CS, Han SS, Kiskinis E, Winborn B, Freibaum BD, Kanagaraj A, Clare AJ, Badders NM, Bilican B, Chaum E, Chandran S, Shaw CE, Eggan KC, Maniatis T, Taylor JP, Axonal transport of TDP-43 mRNA granules is impaired by ALS-causing mutations. Neuron. 2014 Feb 5;81(3):536-43. doi: 10.1016/j.neuron.2013.12.018.

[PubMed ID: 24507183] ... Jovicic A, Gitler AD, TDP-43 in ALS: stay on target...almost there. Neuron. 2014 Feb 5;81(3):463-5. doi: 10.1016/j.neuron.2014.01.034.

[PubMed ID: 17322306] ... Sugiyama N, Masuda T, Shinoda K, Nakamura A, Tomita M, Ishihama Y, Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications. Mol Cell Proteomics. 2007 Jun;6(6):1103-9. Epub 2007 Feb 23.

[PubMed ID: 17081983] ... Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M, Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006 Nov 3;127(3):635-48.

[PubMed ID: 11285240] ... Buratti E, Dork T, Zuccato E, Pagani F, Romano M, Baralle FE, Nuclear factor TDP-43 and SR proteins promote in vitro and in vivo CFTR exon 9 skipping. EMBO J. 2001 Apr 2;20(7):1774-84.

[PubMed ID: 11256614] ... Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S, Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO Rep. 2000 Sep;1(3):287-92.

[PubMed ID: 8599929] ... Tokai N, Fujimoto-Nishiyama A, Toyoshima Y, Yonemura S, Tsukita S, Inoue J, Yamamota T, Kid, a novel kinesin-like DNA binding protein, is localized to chromosomes and the mitotic spindle. EMBO J. 1996 Feb 1;15(3):457-67.

[PubMed ID: 7745706] ... Ou SH, Wu F, Harrich D, Garcia-Martinez LF, Gaynor RB, Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs. J Virol. 1995 Jun;69(6):3584-96.

[PubMed ID: 20301761] ... Harms MM, Miller TM, Baloh RH, TARDBP-Related Amyotrophic Lateral Sclerosis

[PubMed ID: 20301623] ... Kinsley L, Siddique T, Amyotrophic Lateral Sclerosis Overview