SB0144 : Striatal-enriched protein tyrosine phosphatase (STEP)
[ CaMP Format ]
* Basic Information
| Organism | Homo sapiens (human) |
| Protein Names | tyrosine-protein phosphatase non-receptor type 5 isoform c [Homo sapiens]; tyrosine-protein phosphatase non-receptor type 5 isoform c; protein-tyrosine phosphatase striatum-enriched; neural-specific protein-tyrosine phosphatase; tyrosine-protein phosphatase non-receptor type 5; striatum-enriched protein-tyrosine phosphatase; Tyrosine-protein phosphatase non-receptor type 5; 3.1.3.48; Neural-specific protein-tyrosine phosphatase; Striatum-enriched protein-tyrosine phosphatase; STEP |
| Gene Names | PTPN5; protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) |
| Gene Locus | 11p15.1; chromosome 11 |
| GO Function | Not available |
| Entrez Protein | Entrez Nucleotide | Entrez Gene | UniProt | OMIM | HGNC | HPRD | KEGG |
|---|---|---|---|---|---|---|---|
| NP_001265167 | NM_001278238 | 84867 | P54829 | 176879 | HGNC:9657 | N/A | hsa:84867 |
* Information From OMIM
Function: In rat neuronal cell cultures, Paul et al. (2003) showed that glutamate-mediated activation of N-methyl-D-aspartate (NMDA) receptors (see OMIM:138249) leads to the rapid but transient phosphorylation of extracellular signal-related kinase-2 (ERK2; OMIM:176948). NMDA-mediated influx of calcium, but not increased intracellular calcium from other sources, led to activation of the calcium-dependent phosphatase calcineurin and the subsequent dephosphorylation and activation of STEP. STEP then inactivated ERK2 through dephosphorylation of the tyrosine residue in its activation domain and blocked nuclear translocation of the kinase. Thus, STEP is important in regulating the duration of ERK activation and downstream signaling in neurons.
* Structure Information
1. Primary Information
Length: 541 aa
Average Mass: 60.961 kDa
Monoisotopic Mass: 60.922 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| --- cleavage 224 --- | ||||
| Tyrosine phosphatase family 1. | 458 | 488 | 120.0 | 0.0 |
3. Sequence Information
Fasta Sequence: SB0144.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
* Cleavage Information
1 [sites] cleaved by Calpain 1
Source Reference: [PubMed ID: 19625523] Xu J, Kurup P, Zhang Y, Goebel-Goody SM, Wu PH, Hawasli AH, Baum ML, Bibb JA, Lombroso PJ, Extrasynaptic NMDA receptors couple preferentially to excitotoxicity via calpain-mediated cleavage of STEP. J Neurosci. 2009 Jul 22;29(29):9330-43. doi: 10.1523/JNEUROSCI.2212-09.2009.
Cleavage sites (±10aa)
[Site 1] LQERRGSNVS224-LTLDMCTPGC
Ser224 
Leu
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Leu215 | Gln216 | Glu217 | Arg218 | Arg219 | Gly220 | Ser221 | Asn222 | Val223 | Ser224 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Leu225 | Thr226 | Leu227 | Asp228 | Met229 | Cys230 | Thr231 | Pro232 | Gly233 | Cys234 |
Sequence conservation (by blast)
* References
[PubMed ID: 23648065] Low SK, Chung S, Takahashi A, Zembutsu H, Mushiroda T, Kubo M, Nakamura Y, Genome-wide association study of chemotherapeutic agent-induced severe neutropenia/leucopenia for patients in Biobank Japan. Cancer Sci. 2013 Aug;104(8):1074-82. doi: 10.1111/cas.12186. Epub 2013 Jun 10.