SB0150 : CAPN3, p94, calpain-3
[ CaMP Format ]
* Basic Information
| Organism | Homo sapiens (human) |
| Protein Names | calpain-3 isoform a [Homo sapiens]; calpain-3 isoform a; calpain, large polypeptide L3; calpain p94, large [catalytic] subunit; muscle-specific calcium-activated neutral protease 3 large subunit; new calpain 1; Calpain-3; 3.4.22.54; Calcium-activated neutral proteinase 3; CANP 3; Calpain L3; Calpain p94; Muscle-specific calcium-activated neutral protease 3; New calpain 1; nCL-1 |
| Gene Names | CAPN3; CANP3, CANPL3, NCL1; CANP3; CANPL3; NCL1; calpain 3, (p94) |
| Gene Locus | 15q15.1; chromosome 15 |
| GO Function | Not available |
* Information From OMIM
Description: The CAPN3 gene encodes calpain-3. The calpains, or calcium-activated neutral proteases (EC 3.4.22.17), are nonlysosomal intracellular cysteine proteases. Mammalian calpains are heterodimers composed of a ubiquitous 80-kD large subunit (e.g., CAPN1, OMIM:114220 and CAPN2, OMIM:114230) and a common small 30-kD subunit (CAPNS1; OMIM:114170). CAPN3 is a muscle-specific large subunit (Sorimachi et al., 1989).
Function: In COS-1 cells, Huang et al. (2008) demonstrated that calpain-3 and AHNAK (OMIM:103390) colocalize at the I-band near the A-I junction in skeletal muscle, that calpain-3 cleaves AHNAK, and that this cleavage results in decreased levels of AHNAK. Studies of AHNAK fusion protein constructs showed that calpain-3 can cleave AHNAK at 2 sites in the N terminus and 3 sites in the C terminus, but not at the central M region. Cleavage of AHNAK disrupted its binding to dysferlin (DYSF; OMIM:603009) and myoferlin (FER1L3; OMIM:604603). Skeletal muscle from 4 patients with LGMD2A (OMIM:253600) due to CAPN3 mutations showed increased levels of AHNAK at the sarcolemma and blood vessels. Huang et al. (2008) concluded that CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling.
* Structure Information
1. Primary Information
Length: 821 aa
Average Mass: 94.253 kDa
Monoisotopic Mass: 94.194 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| --- cleavage 14 --- | ||||
| --- cleavage 30 --- | ||||
| --- cleavage 33 --- | ||||
| EF-hand domain pair 1. | 394 | 409 | 11.0 | 0.0 |
| --- cleavage 412 --- | ||||
| Calpain large subunit, domain III 1. | 429 | 578 | 1.0 | 0.0 |
| Unstructured region on Calpain-3 1. | 583 | 653 | 1.0 | 10.8 |
| EF-hand domain pair 2. | 668 | 720 | 16.0 | 0.1 |
| EF-hand domain pair 3. | 730 | 744 | 31.0 | 0.0 |
| EF-hand domain pair 4. | 796 | 809 | 32.0 | 0.5 |
3. Sequence Information
Fasta Sequence: SB0150.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
* Cleavage Information
4 [sites] cleaved by Calpain 3
Source Reference: [PubMed ID: 14594950] Ono Y, Kakinuma K, Torii F, Irie A, Nakagawa K, Labeit S, Abe K, Suzuki K, Sorimachi H, Possible regulation of the conventional calpain system by skeletal muscle-specific calpain, p94/calpain 3. J Biol Chem. 2004 Jan 23;279(4):2761-71. Epub 2003 Nov 1.
Cleavage sites (±10aa)
[Site 1] ISASVAPRTA14-AEPRSPGPVP
Ala14 
Ala
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Ile5 | Ser6 | Ala7 | Ser8 | Val9 | Ala10 | Pro11 | Arg12 | Thr13 | Ala14 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ala15 | Glu16 | Pro17 | Arg18 | Ser19 | Pro20 | Gly21 | Pro22 | Val23 | Pro24 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| MPTVISASVAPRTAAEPRSPGPVPHPAQSKATEAGGGNPSGIYS |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Homo sapiens | 99.80 | 3 | calpain 3 isoform c |
| 2 | Macaca fascicularis | 95.90 | 1 | CAN3_MACFA Calpain-3 (Calpain L3) (Calpain p94) ( |
| 3 | Mus musculus | 89.40 | 2 | AF127766_1 calpain 3; calcium activated neutral p |
| 4 | Canis familiaris | 87.80 | 18 | PREDICTED: similar to Calpain-3 (Calpain L3) (Cal |
| 5 | Rattus norvegicus | 86.70 | 1 | calpain 3 |
| 6 | N/A | 86.70 | 1 | 1613155A Ca dependent Cys protease p94 |
| 7 | Ovis aries | 84.30 | 1 | skeletal muscle-specific calpain |
| 8 | Bos taurus | 81.60 | 1 | calpain 3 |
Top-ranked sequences
| organism | matching |
|---|---|
| Homo sapiens | Query 1 MPTVISASVAPRTA#AEPRSPGPVPHPAQSKATEAGGGNPSGIYS 44 ||||||||||||||#|||||||||||||||||||||||||||||| Query 1 MPTVISASVAPRTA#AEPRSPGPVPHPAQSKATEAGGGNPSGIYS 44 |
| Macaca fascicularis | Query 1 MPTVISASVAPRTA#AEPRSPGPVPHPAQSKATEAGGGNPSGIYS 44 ||||||||||||||#|||||||||||||||||||||||| ||||| Sbjct 1 MPTVISASVAPRTA#AEPRSPGPVPHPAQSKATEAGGGNASGIYS 44 |
| Mus musculus | Query 1 MPTVISASVAPRTA#AEPRSPGPVPHPAQSKATEAGGGNPSGIYS 44 |||||| +||||| #|||||||||||||| | ||||||+|||||| Sbjct 1 MPTVISPTVAPRTG#AEPRSPGPVPHPAQGKTTEAGGGHPSGIYS 44 |
| Canis familiaris | Query 1 MPTVISASVAPRTA#AEPRSPGPVPHPAQSKATEAGGGNPSGIYS 44 ||||||||||||| #||||||||+||| | + ||||| ||||||| Sbjct 1 MPTVISASVAPRTR#AEPRSPGPIPHPDQGRTTEAGGANPSGIYS 44 |
| Rattus norvegicus | Query 1 MPTVISASVAPRTA#AEPRSPGPVPHPAQSKATEAGGGNPSGIYS 44 |||||| +||||| #|||||||||||||| | ||||||+| |||| Sbjct 1 MPTVISPTVAPRTG#AEPRSPGPVPHPAQGKTTEAGGGHPGGIYS 44 |
| N/A | Query 1 MPTVISASVAPRTA#AEPRSPGPVPHPAQSKATEAGGGNPSGIYS 44 |||||| +||||| #|||||||||||||| | ||||||+| |||| Sbjct 1 MPTVISPTVAPRTG#AEPRSPGPVPHPAQGKTTEAGGGHPGGIYS 44 |
| Ovis aries | Query 1 MPTVISASVAPRTA#AEPRSPGPVPHPAQSKATEAGGGNPSGIYS 44 ||||||||||||| #||||||||+ || | ||||||||||||| Sbjct 1 MPTVISASVAPRTG#AEPRSPGPIAQAAQGKGTEAGGGNPSGIYS 44 |
| Bos taurus | Query 1 MPTVISASVAPRTA#AEPRSPGPVPHPAQSKATEAGGGNPSGIYS 44 ||||||||||||| #||| ||||+ || | ||||||||||||| Sbjct 1 MPTVISASVAPRTG#AEPMSPGPIAQAAQDKGTEAGGGNPSGIYS 44 |
[Site 2] GPVPHPAQSK30-ATEAGGGNPS
Lys30 Ala
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Gly21 | Pro22 | Val23 | Pro24 | His25 | Pro26 | Ala27 | Gln28 | Ser29 | Lys30 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ala31 | Thr32 | Glu33 | Ala34 | Gly35 | Gly36 | Gly37 | Asn38 | Pro39 | Ser40 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| MPTVISASVAPRTAAEPRSPGPVPHPAQSKATEAGGGNPSGIYSAIISRNFPIIGVKEKT |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Homo sapiens | 131.00 | 3 | calpain 3 isoform c |
| 2 | Macaca fascicularis | 128.00 | 1 | CAN3_MACFA Calpain-3 (Calpain L3) (Calpain p94) ( |
| 3 | Mus musculus | 121.00 | 2 | AF127766_1 calpain 3; calcium activated neutral p |
| 4 | Canis familiaris | 119.00 | 18 | PREDICTED: similar to Calpain-3 (Calpain L3) (Cal |
| 5 | Rattus norvegicus | 119.00 | 1 | calpain 3 |
| 6 | N/A | 119.00 | 1 | 1613155A Ca dependent Cys protease p94 |
| 7 | Ovis aries | 116.00 | 1 | skeletal muscle-specific calpain |
| 8 | Bos taurus | 113.00 | 1 | calpain 3 |
| 9 | Sus scrofa | 88.60 | 1 | calpain 3 |
| 10 | Gallus gallus | 39.30 | 1 | calpain 3, (p94) |
Top-ranked sequences
| organism | matching |
|---|---|
| Homo sapiens | Query 1 MPTVISASVAPRTAAEPRSPGPVPHPAQSK#ATEAGGGNPSGIYSAIISRNFPIIGVKEKT 60 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 1 MPTVISASVAPRTAAEPRSPGPVPHPAQSK#ATEAGGGNPSGIYSAIISRNFPIIGVKEKT 60 |
| Macaca fascicularis | Query 1 MPTVISASVAPRTAAEPRSPGPVPHPAQSK#ATEAGGGNPSGIYSAIISRNFPIIGVKEKT 60 ||||||||||||||||||||||||||||||#|||||||| ||||||||||||||||||||| Sbjct 1 MPTVISASVAPRTAAEPRSPGPVPHPAQSK#ATEAGGGNASGIYSAIISRNFPIIGVKEKT 60 |
| Mus musculus | Query 1 MPTVISASVAPRTAAEPRSPGPVPHPAQSK#ATEAGGGNPSGIYSAIISRNFPIIGVKEKT 60 |||||| +||||| |||||||||||||| |# ||||||+|||||||||||||||||||||| Sbjct 1 MPTVISPTVAPRTGAEPRSPGPVPHPAQGK#TTEAGGGHPSGIYSAIISRNFPIIGVKEKT 60 |
| Canis familiaris | Query 1 MPTVISASVAPRTAAEPRSPGPVPHPAQSK#ATEAGGGNPSGIYSAIISRNFPIIGVKEKT 60 ||||||||||||| ||||||||+||| | +# ||||| ||||||||||||||||||||||| Sbjct 1 MPTVISASVAPRTRAEPRSPGPIPHPDQGR#TTEAGGANPSGIYSAIISRNFPIIGVKEKT 60 |
| Rattus norvegicus | Query 1 MPTVISASVAPRTAAEPRSPGPVPHPAQSK#ATEAGGGNPSGIYSAIISRNFPIIGVKEKT 60 |||||| +||||| |||||||||||||| |# ||||||+| |||||||||||||||||||| Sbjct 1 MPTVISPTVAPRTGAEPRSPGPVPHPAQGK#TTEAGGGHPGGIYSAIISRNFPIIGVKEKT 60 |
| N/A | Query 1 MPTVISASVAPRTAAEPRSPGPVPHPAQSK#ATEAGGGNPSGIYSAIISRNFPIIGVKEKT 60 |||||| +||||| |||||||||||||| |# ||||||+| |||||||||||||||||||| Sbjct 1 MPTVISPTVAPRTGAEPRSPGPVPHPAQGK#TTEAGGGHPGGIYSAIISRNFPIIGVKEKT 60 |
| Ovis aries | Query 1 MPTVISASVAPRTAAEPRSPGPVPHPAQSK#ATEAGGGNPSGIYSAIISRNFPIIGVKEKT 60 ||||||||||||| ||||||||+ || |# ||||||||||||||||||||||||||||| Sbjct 1 MPTVISASVAPRTGAEPRSPGPIAQAAQGK#GTEAGGGNPSGIYSAIISRNFPIIGVKEKT 60 |
| Bos taurus | Query 1 MPTVISASVAPRTAAEPRSPGPVPHPAQSK#ATEAGGGNPSGIYSAIISRNFPIIGVKEKT 60 ||||||||||||| ||| ||||+ || |# ||||||||||||||||||||||||||||| Sbjct 1 MPTVISASVAPRTGAEPMSPGPIAQAAQDK#GTEAGGGNPSGIYSAIISRNFPIIGVKEKT 60 |
| Sus scrofa | Query 1 MPTVISASVAPRTAAEPRSPGPVPHPAQSK#ATEAGGGNPSGIYSAIISRNFPIIGVKEKT 60 ||||||||+|||| | + | +| || |# |||| ||| | |||||||||||||||||| Sbjct 1 MPTVISASMAPRTGAS-QVPRTMPQAAQGK#GTEAGVGNPGGKYSAIISRNFPIIGVKEKT 59 |
| Gallus gallus | Query 1 MPTVISASVAPRTAAEPRSPGPVPHPAQSK#ATEAGGGNPSGIYSAIISRNFPIIGVKEKT 60 ||+ |+|+|| +||| | # || || |||||||||| ||| ||||| Sbjct 1 MPSAINAAVAQQTAAGSVPS------TTST#TTEGTGGGTGGIYSAIISRNQPIIKVKEKT 54 |
[Site 3] PHPAQSKATE33-AGGGNPSGIY
Glu33 Ala
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Pro24 | His25 | Pro26 | Ala27 | Gln28 | Ser29 | Lys30 | Ala31 | Thr32 | Glu33 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ala34 | Gly35 | Gly36 | Gly37 | Asn38 | Pro39 | Ser40 | Gly41 | Ile42 | Tyr43 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| VISASVAPRTAAEPRSPGPVPHPAQSKATEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQ |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Homo sapiens | 131.00 | 3 | calpain 3 isoform c |
| 2 | Macaca fascicularis | 126.00 | 1 | CAN3_MACFA Calpain-3 (Calpain L3) (Calpain p94) ( |
| 3 | Mus musculus | 120.00 | 2 | AF127766_1 calpain 3; calcium activated neutral p |
| 4 | Canis familiaris | 119.00 | 18 | PREDICTED: similar to Calpain-3 (Calpain L3) (Cal |
| 5 | N/A | 117.00 | 1 | 1613155A Ca dependent Cys protease p94 |
| 6 | Rattus norvegicus | 117.00 | 1 | calpain 3 |
| 7 | Ovis aries | 115.00 | 1 | skeletal muscle-specific calpain |
| 8 | Bos taurus | 113.00 | 1 | calpain 3 |
| 9 | Sus scrofa | 88.20 | 1 | calpain 3 |
| 10 | Gallus gallus | 40.00 | 1 | calpain 3, (p94) |
| 11 | Aspergillus oryzae RIB40 | 33.10 | 2 | hypothetical protein |
| 12 | Xenopus tropicalis | 30.80 | 1 | aldehyde dehydrogenase 16 family, member A1 |
Top-ranked sequences
| organism | matching |
|---|---|
| Homo sapiens | Query 4 VISASVAPRTAAEPRSPGPVPHPAQSKATE#AGGGNPSGIYSAIISRNFPIIGVKEKTFEQ 63 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 4 VISASVAPRTAAEPRSPGPVPHPAQSKATE#AGGGNPSGIYSAIISRNFPIIGVKEKTFEQ 63 |
| Macaca fascicularis | Query 4 VISASVAPRTAAEPRSPGPVPHPAQSKATE#AGGGNPSGIYSAIISRNFPIIGVKEKTFEQ 63 ||||||||||||||||||||||||||||||#||||| |||||||||||||||||||||||| Sbjct 4 VISASVAPRTAAEPRSPGPVPHPAQSKATE#AGGGNASGIYSAIISRNFPIIGVKEKTFEQ 63 |
| Mus musculus | Query 4 VISASVAPRTAAEPRSPGPVPHPAQSKATE#AGGGNPSGIYSAIISRNFPIIGVKEKTFEQ 63 ||| +||||| |||||||||||||| | ||#||||+||||||||||||||||||||||||| Sbjct 4 VISPTVAPRTGAEPRSPGPVPHPAQGKTTE#AGGGHPSGIYSAIISRNFPIIGVKEKTFEQ 63 |
| Canis familiaris | Query 4 VISASVAPRTAAEPRSPGPVPHPAQSKATE#AGGGNPSGIYSAIISRNFPIIGVKEKTFEQ 63 |||||||||| ||||||||+||| | + ||#||| |||||||||||||||||||||||||| Sbjct 4 VISASVAPRTRAEPRSPGPIPHPDQGRTTE#AGGANPSGIYSAIISRNFPIIGVKEKTFEQ 63 |
| N/A | Query 4 VISASVAPRTAAEPRSPGPVPHPAQSKATE#AGGGNPSGIYSAIISRNFPIIGVKEKTFEQ 63 ||| +||||| |||||||||||||| | ||#||||+| ||||||||||||||||||||||| Sbjct 4 VISPTVAPRTGAEPRSPGPVPHPAQGKTTE#AGGGHPGGIYSAIISRNFPIIGVKEKTFEQ 63 |
| Rattus norvegicus | Query 4 VISASVAPRTAAEPRSPGPVPHPAQSKATE#AGGGNPSGIYSAIISRNFPIIGVKEKTFEQ 63 ||| +||||| |||||||||||||| | ||#||||+| ||||||||||||||||||||||| Sbjct 4 VISPTVAPRTGAEPRSPGPVPHPAQGKTTE#AGGGHPGGIYSAIISRNFPIIGVKEKTFEQ 63 |
| Ovis aries | Query 4 VISASVAPRTAAEPRSPGPVPHPAQSKATE#AGGGNPSGIYSAIISRNFPIIGVKEKTFEQ 63 |||||||||| ||||||||+ || | ||#|||||||||||||||||||||||||||||| Sbjct 4 VISASVAPRTGAEPRSPGPIAQAAQGKGTE#AGGGNPSGIYSAIISRNFPIIGVKEKTFEQ 63 |
| Bos taurus | Query 4 VISASVAPRTAAEPRSPGPVPHPAQSKATE#AGGGNPSGIYSAIISRNFPIIGVKEKTFEQ 63 |||||||||| ||| ||||+ || | ||#|||||||||||||||||||||||||||||| Sbjct 4 VISASVAPRTGAEPMSPGPIAQAAQDKGTE#AGGGNPSGIYSAIISRNFPIIGVKEKTFEQ 63 |
| Sus scrofa | Query 4 VISASVAPRTAAEPRSPGPVPHPAQSKATE#AGGGNPSGIYSAIISRNFPIIGVKEKTFEQ 63 |||||+|||| | + | +| || | ||#|| ||| | ||||||||||||||||||||| Sbjct 4 VISASMAPRTGAS-QVPRTMPQAAQGKGTE#AGVGNPGGKYSAIISRNFPIIGVKEKTFEQ 62 |
| Gallus gallus | Query 42 IYSAIISRNFPIIGVKEKTFEQ 63 ||||||||| ||| |||||+|+ Sbjct 36 IYSAIISRNQPIIKVKEKTYEE 57 |
| Aspergillus oryzae RIB40 | Query 18 RSPGPVPHPAQSKAT--E#AGGGNPSGIYSAIISRNFPII 54 +| ||||| +| ||| |#| || | +||+| |++ Sbjct 178 KSAGPVPHQSQPKATQVE#ASGGAAPGDLNAIVSVLIPLV 216 |
| Xenopus tropicalis | Query 27 AQSKATE#AGGGNPSGIYSAIISRNFPIIGVKEK 59 || + ||# | | |+ +||+ |||++ + | Sbjct 155 AQLRDTE#MNGWKPLGVIAAIVPWNFPLMLLTWK 187 |
[Site 4] DFIYHFTKLE412-ICNLTADALQ
Glu412 Ile
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Asp403 | Phe404 | Ile405 | Tyr406 | His407 | Phe408 | Thr409 | Lys410 | Leu411 | Glu412 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ile413 | Cys414 | Asn415 | Leu416 | Thr417 | Ala418 | Asp419 | Ala420 | Leu421 | Gln422 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| EKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSDKLQTWTVSVNEGRWVRGC |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Homo sapiens | 132.00 | 12 | calpain 3 isoform a |
| 2 | Macaca fascicularis | 131.00 | 1 | CAN3_MACFA Calpain-3 (Calpain L3) (Calpain p94) ( |
| 3 | Canis familiaris | 130.00 | 22 | PREDICTED: similar to Calpain-3 (Calpain L3) (Cal |
| 4 | N/A | 130.00 | 12 | skeletal muscle-specific calpain |
| 5 | Ovis aries | 130.00 | 2 | skeletal muscle-specific calpain |
| 6 | Bos taurus | 129.00 | 5 | calpain 3 |
| 7 | Sus scrofa | 129.00 | 3 | calpain 3 |
| 8 | Mus musculus | 128.00 | 21 | CAN3_MOUSE Calpain-3 (Calpain L3) (Calpain p94) ( |
| 9 | Rattus norvegicus | 128.00 | 13 | calpain 3 |
| 10 | Oryctolagus cuniculus | 125.00 | 1 | calpain 3, (p94) |
| 11 | Gallus gallus | 111.00 | 3 | calpain 3, (p94) |
| 12 | Tetraodon nigroviridis | 100.00 | 6 | unnamed protein product |
| 13 | Danio rerio | 74.30 | 3 | AF282675_1 calpain 1 |
| 14 | Danio rerio | 74.30 | 1 | calpain 1, large subunit |
| 15 | Canis familiaris | 73.60 | 1 | PREDICTED: similar to Calpain-9 (Digestive tract- |
| 16 | Rattus norvegicus | 72.40 | 1 | calpain 1, large subunit |
| 17 | Stizostedion vitreum vitreum | 72.00 | 1 | putative calpain-like protein |
| 18 | Gallus gallus | 71.20 | 1 | mu-calpain large subunit |
| 19 | Coturnix coturnix | 70.10 | 1 | quail calpain |
| 20 | Homo sapiens | 69.70 | 2 | Calpain 1, (mu/I) large subunit |
| 21 | Macaca mulatta | 69.70 | 2 | PREDICTED: calpain 1, large subunit isoform 4 |
| 22 | synthetic construct | 69.70 | 1 | Homo sapiens calpain 1, (mu/I) large subunit |
| 23 | Pongo pygmaeus | 69.70 | 1 | CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1 |
| 24 | Oncorhynchus mykiss | 69.70 | 1 | gill-specific calpain |
| 25 | Xenopus tropicalis | 66.60 | 3 | CAPN1 protein |
| 26 | Xenopus laevis | 66.60 | 2 | calpain 1, (mu/I) large subunit |
| 27 | Bos grunniens | 66.60 | 1 | calpain 2 |
| 28 | Drosophila melanogaster | 63.90 | 2 | Calpain-B CG8107-PA |
| 29 | Drosophila melanogaster | 63.20 | 3 | calpain |
| 30 | Drosophila pseudoobscura | 63.20 | 1 | GA20829-PA |
| 31 | Xenopus laevis | 63.20 | 1 | mu/m-calpain large subunit |
Top-ranked sequences
| organism | matching |
|---|---|
| Homo sapiens | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 |
| Macaca fascicularis | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 |
| Canis familiaris | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 |||||||||||||||||||+||||||||||#|||||||||+|||||+|||||||||||||| Sbjct 328 EKARLQHQVTEDGEFWMSYDDFIYHFTKLE#ICNLTADALESDKLQSWTVSVNEGRWVRGC 387 |
| N/A | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 |||||||||||||||||||+||||||||||#|||||||||+|||||||||||||||||||| Sbjct 106 EKARLQHQVTEDGEFWMSYDDFIYHFTKLE#ICNLTADALESDKLQTWTVSVNEGRWVRGC 165 |
| Ovis aries | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 |||||||||||||||||||+||||||||||#|||||||||+|||||||||||||||||||| Sbjct 384 EKARLQHQVTEDGEFWMSYDDFIYHFTKLE#ICNLTADALESDKLQTWTVSVNEGRWVRGC 443 |
| Bos taurus | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 |||||||||||||||||||+||||||||||#|||||||||+|||||||||||||||||||| Sbjct 384 EKARLQHQVTEDGEFWMSYDDFIYHFTKLE#ICNLTADALESDKLQTWTVSVNEGRWVRGC 443 |
| Sus scrofa | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 |||||||||||||||||||+||||||||||#|||||||||+|||||||||||||||||||| Sbjct 383 EKARLQHQVTEDGEFWMSYDDFIYHFTKLE#ICNLTADALESDKLQTWTVSVNEGRWVRGC 442 |
| Mus musculus | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 |||||||||||||||||||+||+|||||||#|||||||||+|||||||||||||||||||| Sbjct 383 EKARLQHQVTEDGEFWMSYDDFVYHFTKLE#ICNLTADALESDKLQTWTVSVNEGRWVRGC 442 |
| Rattus norvegicus | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 |||||||||||||||||||+||+|||||||#|||||||||+|||||||||||||||||||| Sbjct 383 EKARLQHQVTEDGEFWMSYDDFVYHFTKLE#ICNLTADALESDKLQTWTVSVNEGRWVRGC 442 |
| Oryctolagus cuniculus | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 |||||||||||||||||||+||++||||||#|||||||||+|||||||||||||||||||| Sbjct 315 EKARLQHQVTEDGEFWMSYDDFVFHFTKLE#ICNLTADALESDKLQTWTVSVNEGRWVRGC 374 |
| Gallus gallus | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 || ||||++ ||||||+| |||+ ||||||#||||| | |++||||||||||||||||||| Sbjct 376 EKIRLQHKIAEDGEFWISLEDFMRHFTKLE#ICNLTPDTLEADKLQTWTVSVNEGRWVRGC 435 |
| Tetraodon nigroviridis | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRGC 442 || +| || ||||||||+||| ++||+|#||||| |||+ ||+ |||||||||||||| Sbjct 329 EKEKLHHQSAEDGEFWMSFEDFKKNYTKIE#ICNLTPDALEDDKIHKWTVSVNEGRWVRGC 388 |
| Danio rerio | Query 384 KARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 + |||++ +||||||||+ ||+ ||+||#||||||||||+ +++ |+ + | | || Sbjct 317 RGRLQNR-SEDGEFWMSFSDFLREFTRLE#ICNLTADALQASQVKKWSTANYNGEWRRG 373 |
| Danio rerio | Query 384 KARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 + |||++ +||||||||+ ||+ ||+||#||||||||||+ +++ |+ + | | || Sbjct 317 RGRLQNR-SEDGEFWMSFSDFLREFTRLE#ICNLTADALQASQVKKWSTANYNGEWRRG 373 |
| Canis familiaris | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRW 438 |+ || | +||||||++ || || |+|#||||| |||+ | | | |+|++| | Sbjct 175 EQKRLCHTALDDGEFWMAFRDFKAHFDKVE#ICNLTPDALEEDALHKWEVTVHQGSW 230 |
| Rattus norvegicus | Query 393 EDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 ||||||||+ ||| |||||#||||| |||+| |+ | + || | || Sbjct 330 EDGEFWMSFRDFIREFTKLE#ICNLTPDALKSRTLRNWNTTFYEGTWRRG 378 |
| Stizostedion vitreum vitreum | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 ++ || |+ +||||||||+ ||+ |+ +||#||||| ||| | + | +| +| | || Sbjct 312 DRERLSHR-SEDGEFWMSFADFLRHYDRLE#ICNLTPDALSDDTVSKWALSKFDGSWRRG 369 |
| Gallus gallus | Query 393 EDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 ||||||||+ ||+ ||+||#||||| ||||| | + | + +| | || Sbjct 330 EDGEFWMSFRDFLREFTRLE#ICNLTPDALQSRKFRKWNTRLYDGSWRRG 378 |
| Coturnix coturnix | Query 389 HQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 | ||||||||+ ||+ |++||#||||| ||| |+| | | || | || Sbjct 319 QQKMEDGEFWMSFRDFMREFSRLE#ICNLTPDALTKDELSRWHTQVFEGTWRRG 371 |
| Homo sapiens | Query 393 EDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 ||||||||+ ||+ ||+||#||||| |||+| ++ | ++ || | || Sbjct 330 EDGEFWMSFRDFMREFTRLE#ICNLTPDALKSRTIRKWNTTLYEGTWRRG 378 |
| Macaca mulatta | Query 393 EDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 ||||||||+ ||+ ||+||#||||| |||+| ++ | ++ || | || Sbjct 330 EDGEFWMSFRDFMREFTRLE#ICNLTPDALKSRTIRKWNTTLYEGTWRRG 378 |
| synthetic construct | Query 393 EDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 ||||||||+ ||+ ||+||#||||| |||+| ++ | ++ || | || Sbjct 330 EDGEFWMSFRDFMREFTRLE#ICNLTPDALKSRTIRKWNTTLYEGTWRRG 378 |
| Pongo pygmaeus | Query 393 EDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 ||||||||+ ||+ ||+||#||||| |||+| ++ | ++ || | || Sbjct 330 EDGEFWMSFRDFMREFTRLE#ICNLTPDALKSRTIRKWNTTLYEGTWRRG 378 |
| Oncorhynchus mykiss | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 || |+ +|||||| +||| ++ |+|#||||| |+| | + | |++ || |+|| Sbjct 289 EKKRILQNSMDDGEFWMEFEDFKANYDKIE#ICNLTPDSLTDDTKRKWEVNMFEGNWIRG 347 |
| Xenopus tropicalis | Query 393 EDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 |||||||++|||+ |++||#||||| ||| + | + | +| | | +| Sbjct 330 EDGEFWMAFEDFLREFSRLE#ICNLTPDALTARKYRKWNTTVFNGNWRKG 378 |
| Xenopus laevis | Query 393 EDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 ||||||||+|||+ |++||#||||| ||| + + + | +| | | +| Sbjct 330 EDGEFWMSFEDFLREFSRLE#ICNLTPDALTARRYRKWNTTVYNGSWRKG 378 |
| Bos grunniens | Query 393 EDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 ||||||||+ ||+ |+++||#||||| | | || + | ++ +| | || Sbjct 32 EDGEFWMSFNDFLRHYSRLE#ICNLTPDTLTSDTYKKWKLTKMDGNWRRG 80 |
| Drosophila melanogaster | Query 394 DGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQT----WTVSVNEGRWVRG 441 |||||||++||+ || ++|#||||+ |+| |+ + | +|+ || | | Sbjct 535 DGEFWMSFQDFLNHFDRVE#ICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSG 586 |
| Drosophila melanogaster | Query 383 EKARLQHQVTEDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQT----WTVSVNEGRW 438 +|| + |||||||++||+ || ++|#||||+ |+| |+ + | +|+ || | Sbjct 353 QKAEIGLTFDRDGEFWMSFQDFLNHFDRVE#ICNLSPDSLTEDQQHSGKRKWEMSMYEGEW 412 Query 439 VRG 441 | Sbjct 413 TPG 415 |
| Drosophila pseudoobscura | Query 394 DGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQT----WTVSVNEGRWVRG 441 |||||||++||+ || ++|#|||| |+| |+ + | +|+ || | | Sbjct 541 DGEFWMSFQDFLNHFDRVE#ICNLNPDSLTEDQQNSSRRKWEMSMFEGEWTSG 592 |
| Xenopus laevis | Query 393 EDGEFWMSYEDFIYHFTKLE#ICNLTADALQSDKLQTWTVSVNEGRWVRG 441 ||||||||+++|+ |++||#||||| | | + | | ++ +| | || Sbjct 323 EDGEFWMSFQEFLRQFSRLE#ICNLTPDTLNKEGLSKWHTTLYDGSWRRG 371 |
* References
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