XSB0278 : N-methyl-D-aspartate receptor subunit [Rattus norvegicus]
[ CaMP Format ]
This entry is computationally expanded from SB0072
* Basic Information
| Organism | Rattus norvegicus (Norway rat) |
| Protein Names | Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A; N-methyl-D-aspartate receptor subunit |
| Gene Names | Grin2a |
| Gene Locus | Not available |
| GO Function | Not available |
| Entrez Protein |
Entrez Nucleotide |
Entrez Gene |
UniProt |
OMIM |
HGNC |
HPRD |
KEGG |
| BAA02498 |
N/A |
N/A |
Q00959 |
N/A |
N/A |
N/A |
rno:24409 |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 1464 aa
Average Mass: 165.517 kDa
Monoisotopic Mass: 165.411 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
| HS1_rep 1. | 179 | 215 | 6.7 | 3.1 |
| Lig_chan-Glu_bd 1. | 439 | 502 | 80.6 | 5.5e-21 |
| Lig_chan 1. | 554 | 838 | 377.4 | 2.5e-110 |
| NMDAR2_C 1. | 839 | 1464 | 1159.4 | 0 |
| --- cleavage 1329 (inside NMDAR2_C 839..1464) --- |
| --- cleavage 1278 (inside NMDAR2_C 839..1464) --- |
3. Sequence Information
Fasta Sequence: XSB0278.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Known Structures in PDB:
2A5S (X-ray; 170 A; A=401-802), 2A5T (X-ray; 200 A; B=401-802)
* Cleavage Information
2 [sites]
Cleavage sites (±10aa)
[Site 1] LEGNLYGSLF1329-SVPSSKLLGN
Phe1329 
Ser
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Leu1320 | Glu1321 | Gly1322 | Asn1323 | Leu1324 | Tyr1325 | Gly1326 | Ser1327 | Leu1328 | Phe1329 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Ser1330 | Val1331 | Pro1332 | Ser1333 | Ser1334 | Lys1335 | Leu1336 | Leu1337 | Gly1338 | Asn1339 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
REIDLSRPSRSISLKDRERLLEGNLYGSLFSVPSSKLLGNKSSLFPQGLEDSKRSKSLLP
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
N/A |
117.00 |
4 |
1 |
| 2 |
Rattus norvegicus |
117.00 |
4 |
N-methyl-D-aspartate receptor NMDAR2A subunit; NMD |
| 3 |
NMDA |
117.00 |
3 |
NMDE1_RAT Glutamate |
| 4 |
Mus musculus |
117.00 |
2 |
glutamate receptor, ionotropic, NMDA2A (epsilon 1) |
| 5 |
Canis familiaris |
112.00 |
1 |
PREDICTED: similar to glutamate receptor, ionotrop |
| 6 |
Saimiri boliviensis |
111.00 |
1 |
glutamate receptor ionotropic N-methyl-D-aspartate |
| 7 |
Homo sapiens |
110.00 |
2 |
N-methyl-D-aspartate receptor subunit 2A precursor |
| 8 |
Pan troglodytes |
110.00 |
1 |
N-methyl-D-aspartate receptor subunit 2A |
| 9 |
Monodelphis domestica |
105.00 |
1 |
PREDICTED: similar to N-methyl-D-aspartate recepto |
| 10 |
Bos taurus |
105.00 |
1 |
PREDICTED: similar to N-methyl-D-aspartate recepto |
| 11 |
Gallus gallus |
85.90 |
1 |
PREDICTED: similar to N-methyl-D-aspartate recepto |
| 12 |
Danio rerio |
72.00 |
2 |
PREDICTED: similar to N-methyl-D-aspartate recepto |
| 13 |
Tetraodon nigroviridis |
50.40 |
1 |
unnamed protein product |
Top-ranked sequences
| organism | matching |
|---|
| N/A |
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
|
| Rattus norvegicus |
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
|
| NMDA |
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
|
| Mus musculus |
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
|
| Canis familiaris |
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
||+|||||||||||||||||||||||||||#||||||| ||||||||||||| ||||||||
Sbjct 1300 REMDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLSGNKSSLFPQGLEDRKRSKSLLP 1359
|
| Saimiri boliviensis |
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
|||||||||||||||||||||||| |||||#||||||| | ||||||||||||||||||||
Sbjct 1224 REIDLSRPSRSISLKDRERLLEGNFYGSLF#SVPSSKLSGKKSSLFPQGLEDSKRSKSLLP 1283
|
| Homo sapiens |
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
||+||||||||||||||||||||| |||||#||||||| | ||||||||||||||||||||
Sbjct 1300 RELDLSRPSRSISLKDRERLLEGNFYGSLF#SVPSSKLSGKKSSLFPQGLEDSKRSKSLLP 1359
|
| Pan troglodytes |
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
||+||||||||||||||||||||| |||||#||||||| | ||||||||||||||||||||
Sbjct 1300 RELDLSRPSRSISLKDRERLLEGNFYGSLF#SVPSSKLSGKKSSLFPQGLEDSKRSKSLLP 1359
|
| Monodelphis domestica |
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
||||| ||||||||||+|||||||||||||#||||||||||| ||| | |||+|||||| |
Sbjct 1305 REIDLGRPSRSISLKDKERLLEGNLYGSLF#SVPSSKLLGNKGSLFSQALEDTKRSKSLFP 1364
|
| Bos taurus |
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
||+| |||||||||||||||||||||||||#||||||| ||| |||||||| ||||||||
Sbjct 1072 REVDPSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLAGNKGSLFPQGLEVGKRSKSLLP 1131
|
| Gallus gallus |
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
||||| ||||||||||+|+ |+ + | |+|#|||||||| ||+|| |||||||||| |
Sbjct 1294 REIDLGRPSRSISLKDKEKFLQSSPYASMF#SVPSSKLLSNKASLLTHALEDSKRSKSLYP 1353
|
| Danio rerio |
Query 1300 REIDLSRPSRSISLKDRERLLEGNLYGSLF#SVPSSKLLGNKSSLFPQGLEDSKRSKSLLP 1359
+||||+||+||+|||+++| || + | ++|#++ + || |++| || + ||+||||||| |
Sbjct 1289 KEIDLNRPARSVSLKEKDRFLEDSPYANMF#NIKADKLFGSRSMLFNRNLEESKRSKSLYP 1348
|
| Tetraodon nigroviridis |
Query 1300 REIDLSRPSRSISLKDRERLLEGNL-YGSLF#SVP--SSKLLG--NKSSLFPQGLEDSKRS 1354
+++|+ ||+||+|||+++| + | +||#++ | +| | +|| +| ||+||||
Sbjct 1216 KDVDVGRPARSVSLKEKDRFATSDSHYANLF#NMRPYSGRLFGTSSKSMMFNHNLEESKRS 1275
Query 1355 KSLLP 1359
||| |
Sbjct 1276 KSLYP 1280
|
[Site 2] QDWSQNNALQ1278-FQKNKLRINR
Gln1278 Phe
|
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Gln1269 | Asp1270 | Trp1271 | Ser1272 | Gln1273 | Asn1274 | Asn1275 | Ala1276 | Leu1277 | Gln1278 |
|
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Phe1279 | Gln1280 | Lys1281 | Asn1282 | Lys1283 | Leu1284 | Arg1285 | Ile1286 | Asn1287 | Arg1288 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
DEDQMLQETGNPATREEVYQQDWSQNNALQFQKNKLRINRQHSYDNILDKPREIDLSRPS
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
NMDA |
127.00 |
6 |
NMDE1_RAT Glutamate |
| 2 |
Rattus norvegicus |
127.00 |
4 |
N-methyl-D-aspartate receptor subunit |
| 3 |
Mus musculus |
124.00 |
2 |
glutamate receptor, ionotropic, NMDA2A (epsilon 1) |
| 4 |
N/A |
121.00 |
5 |
1 |
| 5 |
Saimiri boliviensis |
119.00 |
1 |
glutamate receptor ionotropic N-methyl-D-aspartate |
| 6 |
Homo sapiens |
115.00 |
4 |
N-methyl-D-aspartate receptor subunit 2A precursor |
| 7 |
Pan troglodytes |
115.00 |
2 |
N-methyl-D-aspartate receptor subunit 2A |
| 8 |
Canis familiaris |
112.00 |
1 |
PREDICTED: similar to glutamate receptor, ionotrop |
| 9 |
Bos taurus |
110.00 |
2 |
PREDICTED: similar to N-methyl-D-aspartate recepto |
| 10 |
Monodelphis domestica |
102.00 |
1 |
PREDICTED: similar to N-methyl-D-aspartate recepto |
| 11 |
Gallus gallus |
86.70 |
1 |
PREDICTED: similar to N-methyl-D-aspartate recepto |
| 12 |
Danio rerio |
53.50 |
2 |
PREDICTED: similar to N-methyl-D-aspartate recepto |
| 13 |
Tetraodon nigroviridis |
49.70 |
1 |
unnamed protein product |
| 14 |
Paramecium tetraurelia |
35.00 |
2 |
hypothetical protein |
| 15 |
Macaca mulatta |
34.30 |
1 |
PREDICTED: N-methyl-D-aspartate receptor subunit 2 |
| 16 |
Canis lupus familiaris |
33.90 |
1 |
N-methyl-D-aspartate receptor subunit 2B |
| 17 |
Tetrahymena thermophila SB210 |
32.30 |
1 |
TBC domain containing protein |
Top-ranked sequences
| organism | matching |
|---|
| NMDA |
Query 1249 DEDQMLQETGNPATREEVYQQDWSQNNALQ#FQKNKLRINRQHSYDNILDKPREIDLSRPS 1308
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1249 DEDQMLQETGNPATREEVYQQDWSQNNALQ#FQKNKLRINRQHSYDNILDKPREIDLSRPS 1308
|
| Rattus norvegicus |
Query 1249 DEDQMLQETGNPATREEVYQQDWSQNNALQ#FQKNKLRINRQHSYDNILDKPREIDLSRPS 1308
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1249 DEDQMLQETGNPATREEVYQQDWSQNNALQ#FQKNKLRINRQHSYDNILDKPREIDLSRPS 1308
|
| Mus musculus |
Query 1249 DEDQMLQETGNPATREEVYQQDWSQNNALQ#FQKNKLRINRQHSYDNILDKPREIDLSRPS 1308
||||||||||||||||| ||||||||||||#||||||+|||||||||||||||||||||||
Sbjct 1249 DEDQMLQETGNPATREEAYQQDWSQNNALQ#FQKNKLKINRQHSYDNILDKPREIDLSRPS 1308
|
| N/A |
Query 1249 DEDQMLQETGNPATREEVYQQDWSQNNALQ#FQKNKLRINRQHSYDNILDKPREIDLSRPS 1308
||||||||||||||||| ||||||||||||#||||||+||||+||||||||||||||||||
Sbjct 1249 DEDQMLQETGNPATREEAYQQDWSQNNALQ#FQKNKLKINRQNSYDNILDKPREIDLSRPS 1308
|
| Saimiri boliviensis |
Query 1249 DEDQMLQETGNPATREEVYQQDWSQNNALQ#FQKNKLRINRQHSYDNILDKPREIDLSRPS 1308
||||||||||||||||+||||||+||||||# |||||||+||||||||+||||||||||||
Sbjct 1173 DEDQMLQETGNPATREQVYQQDWAQNNALQ#LQKNKLRISRQHSYDNIVDKPREIDLSRPS 1232
|
| Homo sapiens |
Query 1249 DEDQMLQETGNPATREEVYQQDWSQNNALQ#FQKNKLRINRQHSYDNILDKPREIDLSRPS 1308
|||||||||||||| |+||||||+||||||# |||||||+||||||||+|||||+||||||
Sbjct 1249 DEDQMLQETGNPATGEQVYQQDWAQNNALQ#LQKNKLRISRQHSYDNIVDKPRELDLSRPS 1308
|
| Pan troglodytes |
Query 1249 DEDQMLQETGNPATREEVYQQDWSQNNALQ#FQKNKLRINRQHSYDNILDKPREIDLSRPS 1308
|||||||||||||| |+||||||+||||||# |||||||+||||||||+|||||+||||||
Sbjct 1249 DEDQMLQETGNPATGEQVYQQDWAQNNALQ#LQKNKLRISRQHSYDNIVDKPRELDLSRPS 1308
|
| Canis familiaris |
Query 1249 DEDQMLQETGNPATREEVYQQDWSQNNALQ#FQKNKLRINRQHSYDNILDKPREIDLSRPS 1308
||||||||||+|| +||||||||+||+ ||#||||||||+||||||||+|||||+||||||
Sbjct 1249 DEDQMLQETGSPAAQEEVYQQDWAQNSTLQ#FQKNKLRISRQHSYDNIVDKPREMDLSRPS 1308
|
| Bos taurus |
Query 1249 DEDQMLQETGNPATREEVYQQDWSQNNALQ#FQKNKLRINRQHSYDNILDKPREIDLSRPS 1308
||||||||||||| +||||||||+ |+|||# |||||||+||||||||||||||+| ||||
Sbjct 1021 DEDQMLQETGNPAAQEEVYQQDWAPNSALQ#LQKNKLRISRQHSYDNILDKPREVDPSRPS 1080
|
| Monodelphis domestica |
Query 1249 DEDQMLQET-GNPATREEVYQQDWSQNNALQ#FQK-NKLRINRQHSYDNILDKPREIDLSR 1306
||||||||| ||| || |+|||+||||||#||| |||||+||||||||||||||||| |
Sbjct 1252 DEDQMLQETTANPAHPEETYEQDWAQNNALQ#FQKKNKLRISRQHSYDNILDKPREIDLGR 1311
Query 1307 PS 1308
||
Query 1307 PS 1308
|
| Gallus gallus |
Query 1249 DEDQMLQETGNPATREEVYQQDWSQNNALQ#F-QKNKLRINRQHSYDNILDKPREIDLSRP 1307
+||||||+| | + ||+| ||||||||#+ +||||||+||||+||| || ||||| ||
Sbjct 1242 EEDQMLQDTTNLSLPEEIYGHSWSQNNALQ#YHKKNKLRISRQHSFDNIADKSREIDLGRP 1301
Query 1308 S 1308
|
Query 1308 S 1308
|
| Danio rerio |
Query 1250 EDQMLQETG-NPATR-EEVYQQDWSQNNALQ#FQK-NKLRINRQHSYDNI-LDKPREIDLS 1305
||||||| +|+ ++++ | | + # |+ |+|+++||||+||| |+||+||||+
Sbjct 1235 EDQMLQEAMISPSMHHDDMFSHYWPQTDGPH#VQRRNRLKLSRQHSFDNIMLEKPKEIDLN 1294
Query 1306 RPS 1308
||+
Sbjct 1295 RPA 1297
|
| Tetraodon nigroviridis |
Query 1249 DEDQMLQET-GNPATREEVYQQD-------WSQNNALQ#FQK-NKLRINRQHSYDNI-LDK 1298
+|||+| + |+| + || | | + # || |+||++||||+||| |+|
Sbjct 1155 NEDQLLTDPLGSPTLHHQQQQQADEMFGLYWPQTDGPH#VQKRNRLRLSRQHSFDNIMLEK 1214
Query 1299 PREIDLSRPS 1308
|+++|+ ||+
Sbjct 1215 PKDVDVGRPA 1224
|
| Paramecium tetraurelia |
Query 1253 MLQETGNPATREEVYQQDWSQNNALQ#FQKNKLRINRQHSY--DNILDKPREIDLS 1305
+||+ | ++ ++ || ||#||+|+ +|+| + ++|++| ++||||
Sbjct 165 ILQKQVNDLHKDNIFLGSEKQNLLLQ#FQQNQQFVNQQQNILEEDIINKTQQIDLS 219
|
| Macaca mulatta |
Query 1250 EDQMLQETGNPATREEV--------YQQDWSQNNALQ#FQKNKLRINRQHSYDNILDKPRE 1301
|| ||| || | | | + + | +# +|||| |||||| +| +|
Sbjct 1256 EDNSLQELDQPAAPVAVTSNASTTKYPQSPTNSKAQK#KNRNKLR--RQHSYDTFVDLQKE 1313
|
| Canis lupus familiaris |
Query 1250 EDQMLQETGNPATREEV--------YQQDWSQNNALQ#FQKNKLRINRQHSYDNILDKPRE 1301
|| ||| || | | | + + | +# +|||| |||||| +| +|
Sbjct 1256 EDNSLQELDQPAAPVAVPSNAPSTKYPQSPTNSKAQK#KTRNKLR--RQHSYDTFVDLQKE 1313
|
| Tetrahymena thermophila SB210 |
Query 1249 DEDQMLQETGNPATREEVYQQDWSQNNALQ#FQKNKLRIN 1287
+|+|++|+ |||+|| | |+ |# || ++|
Sbjct 648 NENQLIQQQKPPATQEEDYSVPQELQNSFQ#MNKNMSKLN 686
|
* References
[PubMed ID: 8428958] Ishii T, Moriyoshi K, Sugihara H, Sakurada K, Kadotani H, Yokoi M, Akazawa C, Shigemoto R, Mizuno N, Masu M, et al., Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits. J Biol Chem. 1993 Feb 5;268(4):2836-43.
