CaMPDB :: Substrate

XSB0426 : spectrin, beta, erythrocytic [Rattus norvegicus]

This entry is computationally expanded from SB0078

* Basic Information

Organism	Rattus norvegicus (Norway rat)
Protein Names	Erythroid spectrin beta; spectrin; beta; erythrocytic; spectrin beta 1
Gene Names	Sptb; LOC314251; spectrin, beta, erythrocytic
Gene Locus	6q24; chromosome 6
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_997687	NM_212522	314251	Q6XDA0	N/A	N/A	N/A	rno:314251

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 2137 aa

Average Mass: 246.329 kDa

Monoisotopic Mass: 246.176 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
CH 1.	55	158	118.2	2.7e-32
CH 2.	174	278	147.7	3.6e-41
Spectrin 1.	302	412	69.4	1.3e-17
Spectrin 2.	422	526	109.3	1.3e-29
Spectrin 3.	528	635	92.8	1.2e-24
Spectrin 4.	637	741	127.0	6e-35
Spectrin 5.	743	846	94.7	3.2e-25
Spectrin 6.	848	952	58.7	2.1e-14
Spectrin 7.	954	1059	73.9	5.8e-19
Spectrin 8.	1061	1166	88.9	1.8e-23
Spectrin 9.	1168	1272	15.0	0.0019
Spectrin 10.	1274	1377	112.0	2e-30
Spectrin 11.	1381	1476	21.8	0.00049
Spectrin 12.	1478	1582	79.6	1.1e-20
Spectrin 13.	1584	1688	90.0	8.6e-24
Spectrin 14.	1690	1795	114.8	2.8e-31
Spectrin 15.	1797	1901	85.3	2.2e-22
Spectrin 16.	1903	2007	100.5	5.8e-27
Spectrin 17.	2009	2117	25.4	0.00023
--- cleavage 2058 (inside Spectrin 2009..2117) ---

3. Sequence Information

Fasta Sequence: XSB0426.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites]

Cleavage sites (±10aa)

[Site 1] KRHEAFEKST²⁰⁵⁸-ASWAERFAAL

Thr²⁰⁵⁸ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Lys²⁰⁴⁹	Arg²⁰⁵⁰	His²⁰⁵¹	Glu²⁰⁵²	Ala²⁰⁵³	Phe²⁰⁵⁴	Glu²⁰⁵⁵	Lys²⁰⁵⁶	Ser²⁰⁵⁷	Thr²⁰⁵⁸

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala²⁰⁵⁹	Ser²⁰⁶⁰	Trp²⁰⁶¹	Ala²⁰⁶²	Glu²⁰⁶³	Arg²⁰⁶⁴	Phe²⁰⁶⁵	Ala²⁰⁶⁶	Ala²⁰⁶⁷	Leu²⁰⁶⁸

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
EPYLASRDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQTPDRPTEE

Summary

#	organism	max score	hits	top seq
1	Mus musculus	122.00	47	mKIAA4219 protein
2	Homo sapiens	112.00	61	spectrin, beta, erythrocytic (includes spherocytos
3	N/A	112.00	18	-
4	Danio rerio	80.90	9	spectrin, beta, erythrocytic
5	Gallus gallus	79.00	5	PREDICTED: similar to spectrin, beta, non-erythroc
6	Canis familiaris	77.80	18	PREDICTED: similar to spectrin, beta, non-erythroc
7	Pan troglodytes	77.80	14	PREDICTED: spectrin, beta, non-erythrocytic 1 isof
8	Rattus norvegicus	77.80	13	spectrin beta 2
9	Macaca mulatta	77.80	5	PREDICTED: spectrin, beta, non-erythrocytic 1 isof
10	Bos taurus	77.80	5	PREDICTED: similar to spectrin, beta, non-erythroc
11	Macaca fascicularis	77.80	2	unnamed protein product
12	mice, brain, Peptide Partial, 332 aa	77.80	1	beta-spectrin subunit
13	Aedes aegypti	74.70	3	beta chain spectrin
14	Apis mellifera	74.30	3	PREDICTED: similar to Spectrin beta chain
15	Drosophila melanogaster	73.90	7	beta Spectrin CG5870-PA
16	Drosophila pseudoobscura	73.90	3	GA19192-PA
17	Anopheles gambiae str. PEST	73.90	3	ENSANGP00000012507
18	Tetraodon nigroviridis	72.00	8	unnamed protein product
19	Tribolium castaneum	72.00	4	PREDICTED: similar to Spectrin beta chain isoform
20	Caenorhabditis briggsae	68.20	2	Hypothetical protein CBG19038
21	Brugia malayi	65.90	1	beta-G spectrin
22	Caenorhabditis elegans	65.10	5	UNCoordinated family member (unc-70)
23	Strongylocentrotus purpuratus	63.90	5	PREDICTED: similar to spectrin beta 2
24	Sus scrofa	44.30	1	spectrin alpha non-erythrocytic 1
25	Monodelphis domestica	37.40	4	PREDICTED: similar to spectrin, alpha, erythrocyti
26	Mus sp.	34.70	1	SPTB1_MOUSE Spectrin beta chain, erythrocyte (Beta
27	Xenopus tropicalis	32.30	1	hypothetical protein LOC100036647

Top-ranked sequences

organism	matching
Mus musculus	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPDRPTEE 2088 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\| Sbjct 618 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPERPTEE 677
Homo sapiens	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPDRPTEE 2088 \|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\| \|\| Sbjct 1401 EPYLASGDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQIAERPAEE 1460
N/A	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPDRPTEE 2088 \|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\| \|\| Sbjct 975 EPYLASGDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQIAERPAEE 1034
Danio rerio	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKE 2078 \|\|\|+\|\|+\| \| \|\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|#\|+\| \|\|\|+\|\|\|+ \|\|\|\|\| \| Sbjct 2048 EPYVASKDVGQTVDEVEKLLKRHEAFEKST#ATWEERFSALERLTTLELLE 2097
Gallus gallus	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKE 2078 \|\|\|\|+\|\|+ \| +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| #\|+\| \|\|\|\|\|\|\|+ \|\|\|\|\| \| Sbjct 2036 EPYLSSREIGQSVDEVEKLIKRHEAFEKSA#ATWDERFAALERLTTLELLE 2085
Canis familiaris	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKE 2078 \|\|\|\|+\|\|+ \| +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| #\|+\| \|\|\|+\|\|\|+ \|\|\|\|\| \| Sbjct 2037 EPYLSSREIGQSVDEVEKLIKRHEAFEKSA#ATWDERFSALERLTTLELLE 2086
Pan troglodytes	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKE 2078 \|\|\|\|+\|\|+ \| +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| #\|+\| \|\|\|+\|\|\|+ \|\|\|\|\| \| Sbjct 2037 EPYLSSREIGQSVDEVEKLIKRHEAFEKSA#ATWDERFSALERLTTLELLE 2086
Rattus norvegicus	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKE 2078 \|\|\|\|+\|\|+ \| +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| #\|+\| \|\|\|+\|\|\|+ \|\|\|\|\| \| Sbjct 2032 EPYLSSREIGQSVDEVEKLIKRHEAFEKSA#ATWDERFSALERLTTLELLE 2081
Macaca mulatta	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKE 2078 \|\|\|\|+\|\|+ \| +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| #\|+\| \|\|\|+\|\|\|+ \|\|\|\|\| \| Sbjct 2024 EPYLSSREIGQSVDEVEKLIKRHEAFEKSA#ATWDERFSALERLTTLELLE 2073
Bos taurus	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKE 2078 \|\|\|\|+\|\|+ \| +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| #\|+\| \|\|\|+\|\|\|+ \|\|\|\|\| \| Sbjct 2036 EPYLSSREIGQSVDEVEKLIKRHEAFEKSA#ATWDERFSALERLTTLELLE 2085
Macaca fascicularis	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKE 2078 \|\|\|\|+\|\|+ \| +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| #\|+\| \|\|\|+\|\|\|+ \|\|\|\|\| \| Sbjct 367 EPYLSSREIGQSVDEVEKLIKRHEAFEKSA#ATWDERFSALERLTTLELLE 416
mice, brain, Peptide Partial, 332 aa	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKE 2078 \|\|\|\|+\|\|+ \| +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| #\|+\| \|\|\|+\|\|\|+ \|\|\|\|\| \| Sbjct 163 EPYLSSREIGQSVDEVEKLIKRHEAFEKSA#ATWDERFSALERLTTLELLE 212
Aedes aegypti	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPDRPTEE 2088 \|\|\|\| \| + \|\|\|+\| \|\| \|\|\|+\|\|\|\|\|\|\| #\|+ \|\|\|+\|\|\|+ \|\| \|\|\|\| + \|\| Sbjct 2038 EPYLMSTELGHTIDEVENLIKKHEAFEKSA#AAQEERFSALERLTTFELKEMKRRQEAAEE 2097
Apis mellifera	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPDRPTEE 2088 \|\|\|\| \|++ \|\|\|+\| \|\| \|\|\|+\|\|\|\|\|\|\| #\|+ \|\|\|+\|\| + \|\| \|\|\|\| + ++ \|\| Sbjct 2036 EPYLMSQELGHTIDEVENLIKKHEAFEKSA#AAQEERFSALHRLTTFELKEMKRREQEREE 2095
Drosophila melanogaster	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPDRPTEE 2088 \|\|\|\| \| + \|\|\|+\| \|\| \|\|\|+\|\|\|\|\|\|\| #\|+ \|\|\|+\|\|\|+ \|\| \|\|\|\| + \|\| Sbjct 2038 EPYLLSSELGHTIDEVENLIKKHEAFEKSA#AAQEERFSALERLTTFELKEMKRRQELAEE 2097
Drosophila pseudoobscura	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPDRPTEE 2088 \|\|\|\| \| + \|\|\|+\| \|\| \|\|\|+\|\|\|\|\|\|\| #\|+ \|\|\|+\|\|\|+ \|\| \|\|\|\| + \|\| Sbjct 2038 EPYLLSSELGHTIDEVENLIKKHEAFEKSA#AAQEERFSALERLTTFELKEMKRRQELAEE 2097
Anopheles gambiae str. PEST	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPDRPTEE 2088 \|\|\|\| \| + \|\|\|+\| \|\| \|\|\|+\|\|\|\|\|\|\| #\|+ \|\|\|+\|\|\|+ \|\| \|\|\|\| + \|\| Sbjct 2038 EPYLMSTELGHTIDEVENLIKKHEAFEKSA#AAQEERFSALERLTTFELKEMKRRQDAAEE 2097
Tetraodon nigroviridis	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQ 2080 \|\|\|\|+\|\|+ \| +\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\| #\|+\| \|\|\|+\|\|\|+ \|\|+ \|+ Sbjct 1937 EPYLSSREVGRSVDQVEKLLKRHEAFEKSA#ATWEERFSALERLTTVSAGPRR 1988
Tribolium castaneum	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQ 2080 \|\|\|\| \| + \|\|\|+\| \|\| \|\|\|+\|\|\|\|\|\|\| #\|+ \|\|\|+\|\|\|+ \|\| \|\|+\| \| Sbjct 2037 EPYLMSTELGHTIDDVENLIKKHEAFEKSA#AAQEERFSALERLTTFELREIQ 2088
Caenorhabditis briggsae	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPDRPTEE 2088 \|\|\|\| \|\|++\| ++ \|\|\|\|+\|\|\|\|\|\|\| # + \|\|\| \|\|\|\| \|\| \|\|\|\| \| + \|++ Sbjct 2024 EPYLISREYGRNLEETIKLIKKHEAFEKSA#LAQEERFLALEKLTTFELKENQHREEETQK 2083
Brugia malayi	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPD 2083 \|\|\|\| \|+++\| ++ \|\|\|\|+\|\|\|\|\|\|\| # + \|\|\| \|\|\|\| \|\| \|\|\|\| \| \| Sbjct 2033 EPYLVSKEYGRNLEETIKLIKKHEAFEKSA#NAQEERFLALEKLTTFELKEMQRRD 2087
Caenorhabditis elegans	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPDRPT 2086 \|\|\|\| \|+++\| ++ \|\|\|\|+\|\|\|\|\|\|\| # + \|\|\| \|\|\|\| \|\| \|\|\|\| \| + \| Sbjct 2027 EPYLISKEYGRNLEETIKLIKKHEAFEKSA#FAQEERFLALEKLTTFELKETQHREEET 2084
Strongylocentrotus purpuratus	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKE 2078 \| \|\| \| \|+\| ++\| \|\|\|\|\|\|\|\|\|\|\|\|\|+ # \|\|\| \|\|\|\| \|\| \|\|+\| Sbjct 2026 EQYLRSDDYGDSLDEVEKLIKRHEAFEKAL#YCQEERFTALEKLTTFELRE 2075
Sus scrofa	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTT 2073 \| +\| + \| \| ++\|\|\|\| \|+\|+\|\| \|\|\|\| #++ \|+ \|\|++ \| Sbjct 24 EAFLLNEDLGDSLDSVEALLKKHEDFEKSL#SAQEEKITALDEFVT 68
Monodelphis domestica	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTT 2073 \| +\| + \| \|+++ +\|\| \|+++\|\| \|\|++ # + \|+ \|+\| \| Sbjct 487 EAFLENEDLGNSLGTVEALLQKHEDFEEAF#GAQEEKITTLDKTAT 531
Mus sp.	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPDRPTEE 2088 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\| Sbjct 2020 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTTLELKERQTPERPTEE 2079 Query 2036 DFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALE 2069 +\|\|+ + +\|\| \|+\|\|\| \| \|#\|++ \|\| \|\|\| Sbjct 441 NFGYDLAAVEAAKKKHEAIETDT#AAYEERVKALE 474
Xenopus tropicalis	Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEKPTT 2073 \| +\| + \| \| ++\|\|\|\| \|+\|+\|\| \|\|\|\| #++ \|+ \|\|++ \| Sbjct 487 EAFLLNEDLGDSLDSVEALLKKHEDFEKSL#SAQEEKITALDEFAT 531 Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALE 2069 \| +\| \| \| \| ++\|\|\|\| \|\|\|+\|\| \|+\|+ # \|+ \|\|\|+ Sbjct 1464 EAFLNSEDKGDSLDSVEALIKKHEDFDKAI#NVQEEKIAALQ 1504 Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAAL 2068 \| +\|\| \|\|\| + \|\|+ \|+++\|\| \|+ #\|+ \|+ \|\| Sbjct 275 EQLMASDDFGRDLASVQALLRKHEGLERDL#AALEEKVKAL 314 Query 2029 EPYLASRDFGHTVDSVEKLIKRHEAFEKST#ASWAERFAALEK 2070 \|\| + \| \|+\| \|\| \| \|+\|+\|\|\| # ++ \| + Sbjct 910 EPIVGSTDYGKDEDSAEALLKKHEALMSDL#RAYGSSIQGLRE 951 Query 2032 LASRDFGHTVDSVEKLIKRHEAFE 2055 +\|\| \|+\| \|+ +++ \|+\|+\|\|\|\|\| Sbjct 1892 VASEDYGDTLAAIQGLLKKHEAFE 1915

* References

None.