* Structure Information
1. Primary Information
Length: 271 aa
Average Mass: 30.337 kDa
Monoisotopic Mass: 30.317 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
| IGFBP 1. | 26 | 84 | 102.7 | 1.3e-27 |
| --- cleavage 160 --- |
| --- cleavage 171 --- |
| Thyroglobulin_1 1. | 191 | 262 | 133.7 | 6e-37 |
3. Sequence Information
Fasta Sequence: XSB0439.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
2 [sites]
Cleavage sites (±10aa)
[Site 1] VKKDRRKKLT160-QSKFVGGAEN
Thr160 
Gln
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Val151 | Lys152 | Lys153 | Asp154 | Arg155 | Arg156 | Lys157 | Lys158 | Leu159 | Thr160 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Gln161 | Ser162 | Lys163 | Phe164 | Val165 | Gly166 | Gly167 | Ala168 | Glu169 | Asn170 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
YSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVILAPEMRQESEQGP
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Sus scrofa |
122.00 |
2 |
insulin-like growth factor binding protein 5 |
| 2 |
N/A |
122.00 |
1 |
2 |
| 3 |
Macaca mulatta |
120.00 |
3 |
PREDICTED: similar to insulin-like growth factor b |
| 4 |
Homo sapiens |
120.00 |
2 |
insulin-like growth factor binding protein 5 |
| 5 |
Canis familiaris |
120.00 |
1 |
PREDICTED: similar to insulin-like growth factor b |
| 6 |
synthetic construct |
120.00 |
1 |
insulin-like growth factor binding protein 5 |
| 7 |
Mus musculus |
119.00 |
3 |
insulin-like growth factor binding protein 5 |
| 8 |
Bos taurus |
119.00 |
2 |
PREDICTED: similar to Insulin-like growth factor-b |
| 9 |
Rattus norvegicus |
118.00 |
1 |
insulin-like growth factor binding protein 5 |
| 10 |
Monodelphis domestica |
103.00 |
2 |
PREDICTED: similar to insulin-like growth factor b |
| 11 |
Gallus gallus |
91.30 |
2 |
PREDICTED: similar to insulin-like growth factor b |
| 12 |
Coturnix japonica |
91.30 |
1 |
AF293839_1 insulin-like growth factor binding prot |
Top-ranked sequences
| organism | matching |
|---|
| Sus scrofa |
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
|
| N/A |
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
|
| Macaca mulatta |
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
||||||||||||||||||||||||||||||#||||||||||||||||| ||||||||||||
Sbjct 132 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVISAPEMRQESEQGP 191
|
| Homo sapiens |
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
||||||||||||||||||||||||||||||#|||||||||||||||+| ||||||||||||
Sbjct 132 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRIISAPEMRQESEQGP 191
|
| Canis familiaris |
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
||||||||||||||||||||||||||||||#||||||||||||||||| ||||||||||||
Sbjct 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVISAPEMRQESEQGP 190
|
| synthetic construct |
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
||||||||||||||||||||||||||||||#|||||||||||||||+| ||||||||||||
Sbjct 132 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRIISAPEMRQESEQGP 191
|
| Mus musculus |
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
||||+|||||||||||||||||||||||||#||||||||||||||||| ||||||||||||
Sbjct 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190
|
| Bos taurus |
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
||||||||||||||||||||||||||||||#||||||||||||||||| ||||||+|||||
Sbjct 136 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVISAPEMRQKSEQGP 195
|
| Rattus norvegicus |
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
||||+|||||||||||||||||||||||||#||||||||||||||||| ||||||||+|||
Sbjct 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESDQGP 190
|
| Monodelphis domestica |
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
||||+||||| +||+||||+|||||||||#|||||| ||||| ||| |||||||||||
Sbjct 131 YSPKVFRPKHVHLSEMKAEALKKDRRKKLT#QSKFVGAAENTAQSRVISVPEMRQESEQGP 190
|
| Gallus gallus |
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
| || +|||| |+|+|||||+|||||||||# +|||| |||||||||++ ||+||| | ||
Sbjct 45 YPPKAYRPKHGRLSDLKAEALKKDRRKKLT#LAKFVGMAENTAHPRVVI-PELRQEFELGP 103
|
| Coturnix japonica |
Query 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190
| || +|||| |+|+|||||+|||||||||# +|||| |||||||||++ ||+||| | ||
Sbjct 88 YPPKAYRPKHGRLSDLKAEALKKDRRKKLT#LAKFVGMAENTAHPRVVI-PELRQEFELGP 146
|
[Site 2] SKFVGGAENT171-AHPRVILAPE
Thr171 Ala
|
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Ser162 | Lys163 | Phe164 | Val165 | Gly166 | Gly167 | Ala168 | Glu169 | Asn170 | Thr171 |
|
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Ala172 | His173 | Pro174 | Arg175 | Val176 | Ile177 | Leu178 | Ala179 | Pro180 | Glu181 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
RISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVILAPEMRQESEQGPCRRHMEASLQE
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Sus scrofa |
121.00 |
2 |
insulin-like growth factor binding protein 5 |
| 2 |
N/A |
121.00 |
1 |
2 |
| 3 |
Macaca mulatta |
119.00 |
3 |
PREDICTED: similar to insulin-like growth factor b |
| 4 |
Canis familiaris |
119.00 |
1 |
PREDICTED: similar to insulin-like growth factor b |
| 5 |
Mus musculus |
118.00 |
5 |
insulin-like growth factor binding protein 5 |
| 6 |
Homo sapiens |
118.00 |
2 |
IGFBP5 |
| 7 |
synthetic construct |
118.00 |
1 |
insulin-like growth factor binding protein 5 |
| 8 |
Bos taurus |
117.00 |
1 |
PREDICTED: similar to Insulin-like growth factor-b |
| 9 |
Rattus norvegicus |
117.00 |
1 |
insulin-like growth factor binding protein 5 |
| 10 |
Monodelphis domestica |
104.00 |
2 |
PREDICTED: similar to insulin-like growth factor b |
| 11 |
Gallus gallus |
97.40 |
2 |
PREDICTED: similar to insulin-like growth factor b |
| 12 |
Coturnix japonica |
97.40 |
1 |
AF293839_1 insulin-like growth factor binding prot |
| 13 |
Xenopus laevis |
38.90 |
1 |
insulin-like growth factor binding protein 5 |
| 14 |
Xenopus tropicalis |
38.10 |
1 |
Unknown (protein for MGC:144994) |
| 15 |
Tetraodon nigroviridis |
33.90 |
1 |
unnamed protein product |
| 16 |
Oreochromis mossambicus |
32.70 |
1 |
AF406954_1 insulin-like growth factor binding prot |
| 17 |
Aquifex pyrophilus |
32.30 |
1 |
NAD-dependent DNA ligase |
Top-ranked sequences
| organism | matching |
|---|
| Sus scrofa |
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
|
| N/A |
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
|
| Macaca mulatta |
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
||||||||||||||||||||||||||||||#|||||| |||||||||||||||||||||||
Sbjct 143 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVISAPEMRQESEQGPCRRHMEASLQE 202
|
| Canis familiaris |
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
||||||||||||||||||||||||||||||#|||||| |||||||||||||||||||||||
Sbjct 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVISAPEMRQESEQGPCRRHMEASLQE 201
|
| Mus musculus |
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
||||||||||||||||||||||||||||||#|||||| |||||||||||||||||||||||
Sbjct 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201
|
| Homo sapiens |
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
||||||||||||||||||||||||||||||#||||+| |||||||||||||||||||||||
Sbjct 143 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRIISAPEMRQESEQGPCRRHMEASLQE 202
|
| synthetic construct |
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
||||||||||||||||||||||||||||||#||||+| |||||||||||||||||||||||
Sbjct 143 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRIISAPEMRQESEQGPCRRHMEASLQE 202
|
| Bos taurus |
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
||||||||||||||||||||||||||||||#|||||| ||||||+||||||||||||||||
Sbjct 147 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVISAPEMRQKSEQGPCRRHMEASLQE 206
|
| Rattus norvegicus |
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
||||||||||||||||||||||||||||||#|||||| ||||||||+||||||||||||||
Sbjct 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESDQGPCRRHMEASLQE 201
|
| Monodelphis domestica |
Query 143 ISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
+||+||||+||||||||||||||| ||||#| ||| ||||||||||||||||||||||
Sbjct 143 LSEMKAEALKKDRRKKLTQSKFVGAAENT#AQSRVISVPEMRQESEQGPCRRHMEASLQE 201
|
| Gallus gallus |
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
|+|+|||||+||||||||| +|||| ||||#|||||++ ||+||| | |||||||||||||
Sbjct 56 RLSDLKAEALKKDRRKKLTLAKFVGMAENT#AHPRVVI-PELRQEFELGPCRRHMEASLQE 114
|
| Coturnix japonica |
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
|+|+|||||+||||||||| +|||| ||||#|||||++ ||+||| | |||||||||||||
Sbjct 99 RLSDLKAEALKKDRRKKLTLAKFVGMAENT#AHPRVVI-PELRQEFELGPCRRHMEASLQE 157
|
| Xenopus laevis |
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
|+|+ || |+ |+|| +||+ | | # | || | + ||||| +| +||
Sbjct 140 RLSDQKAIALNTFRQKKHSQSRIVS-VEKV#QSPST---PEHSIEIDMGPCRRQVETLMQE 195
|
| Xenopus tropicalis |
Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201
|+++ || |+ |+|| +||+ | | # | || | + ||||| +| +||
Sbjct 136 RLTDQKAIALNTFRQKKQSQSRIVS-VEKV#QSPST---PEHSIEIDMGPCRRQVETLMQE 191
|
| Tetraodon nigroviridis |
Query 149 EAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASL 199
+ ++||+ || | + |# + | ++||| ||||| ||+ |
Sbjct 152 QLLQKDQNKKTQSYKVEAVSRRT#ITDVQNFSLENKRESEYGPCRREMESIL 202
|
| Oreochromis mossambicus |
Query 147 KAEAVK--KDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASL 199
||| +| +| | + ++ | + || |# +||+| ||||| ||+ |
Sbjct 119 KAEVIKNAEDNRSQRSKVKPLPGAVGT#DGQNFSCGS--KQETENGPCRREMESVL 171
|
| Aquifex pyrophilus |
Query 142 RISELKAEAV-KKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEA 197
+++|++ | | |+ ||| | + | #|+|| | +||+ + +| +||
Sbjct 194 KLAEIRGEVVINKEEFKKLNQERIEEGLPPF#ANPRNAAAGSIRQKDPKEVAKRRLEA 250
|
* References
[PubMed ID: 15001525] Xu Q, Li S, Zhao Y, Maures TJ, Yin P, Duan C, Evidence that IGF binding protein-5 functions as a ligand-independent transcriptional regulator in vascular smooth muscle cells. Circ Res. 2004 Mar 19;94(5):E46-54. Epub 2004 Mar 4.
» More (1) « Hide Below
[PubMed ID: 8573141] ... White ME, Diao R, Hathaway MR, Mickelson J, Dayton WR, Molecular cloning and sequence analysis of the porcine insulin-like growth factor binding protein-5 complementary deoxyribonucleic acid. Biochem Biophys Res Commun. 1996 Jan 5;218(1):248-53.
