CaMPDB :: Substrate

XSB0558 : caspase 9, apoptosis-related cysteine peptidase [Homo sapiens]

This entry is computationally expanded from SB0042

* Basic Information

Organism	Homo sapiens (human)
Protein Names	Caspase 9, apoptosis-related cysteine peptidase; caspase 9; apoptosis-related cysteine peptidase
Gene Names	CASP9; RP11-265F14.3-003
Gene Locus	Not available
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
CAI12972	N/A	N/A	Q5JRU2	N/A	1511	N/A	hsa:

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 266 aa

Average Mass: 28.765 kDa

Monoisotopic Mass: 28.746 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
--- cleavage 32 ---
Peptidase_C14 1.	78	259	134.2	4.3e-37
--- cleavage 247 (inside Peptidase_C14 78..259) ---

3. Sequence Information

Fasta Sequence: XSB0558.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

2 [sites]

Cleavage sites (±10aa)

[Site 1] EGLRTFDQLD²⁴⁷-AISSLPTPSD

Asp²⁴⁷ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Glu²³⁸	Gly²³⁹	Leu²⁴⁰	Arg²⁴¹	Thr²⁴²	Phe²⁴³	Asp²⁴⁴	Gln²⁴⁵	Leu²⁴⁶	Asp²⁴⁷

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala²⁴⁸	Ile²⁴⁹	Ser²⁵⁰	Ser²⁵¹	Leu²⁵²	Pro²⁵³	Thr²⁵⁴	Pro²⁵⁵	Ser²⁵⁶	Asp²⁵⁷

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
TSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFP

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	104.00	8	caspase-9
2	N/A	104.00	4	B
3	Pan troglodytes	104.00	3	PREDICTED: caspase 9 isoform 2
4	synthetic construct	104.00	1	Homo sapiens caspase 9, apoptosis-related cysteine
5	Macaca mulatta	98.20	1	PREDICTED: similar to caspase 9 isoform alpha prep
6	Macaca fascicularis	98.20	1	caspase 9
7	Felis catus	85.90	1	CASP9
8	Canis familiaris	85.50	2	PREDICTED: caspase 9, apoptosis-related cysteine p
9	Canis lupus familiaris	85.50	1	caspase 9
10	Rattus norvegicus	74.70	1	caspase 9
11	Monodelphis domestica	72.80	1	PREDICTED: similar to ICE-LAP6
12	Mus musculus	69.70	6	caspase9S
13	Gallus gallus	55.10	1	caspase 9
14	Oryctolagus cuniculus	52.00	1	caspase 9
15	Dicentrarchus labrax	50.80	1	caspase-9
16	Xenopus laevis	49.30	1	caspase-9
17	Danio rerio	48.10	2	Zgc:101776 protein
18	Oncorhynchus mykiss	43.10	1	caspase-9
19	Tetraodon nigroviridis	42.70	1	unnamed protein product
20	Aspergillus clavatus NRRL 1	32.70	1	pentatricopeptide repeat protein

Top-ranked sequences

organism	matching
Homo sapiens	Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266
N/A	Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266
Pan troglodytes	Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266
synthetic construct	Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266
Macaca mulatta	Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|#\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 301 TSPEDESSGSNPEPDATPFQEDLRTFDQLD#AVSSLPTPSDIFVSYSTFP 349
Macaca fascicularis	Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|#\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 287 TSPEDESSGSNPEPDATPFQEDLRTFDQLD#AVSSLPTPSDIFVSYSTFP 335
Felis catus	Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\|\|\|\| +\|\|\|+\|\|\|\|\| \|\|\|\| \|\|\|\| \|#\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 254 TSPEDRTPGSDPEPDAVAFQEGPGTFDQPD#AVSSLPTPSDIFVSYSTFP 302
Canis familiaris	Query 219 SPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\|\|\| \|\|\|\|+ \|\|\|\| \|\|\|\|\| \|\|\|\|\|#\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 301 SPEDRSPGSDSEPDAVPFQEGPGPFDQLD#AVSSLPTPSDIFVSYSTFP 348
Canis lupus familiaris	Query 219 SPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\|\|\| \|\|\|\|+ \|\|\|\| \|\|\|\|\| \|\|\|\|\|#\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 332 SPEDRSPGSDSEPDAVPFQEGPGPFDQLD#AVSSLPTPSDIFVSYSTFP 379
Rattus norvegicus	Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\| +\|++ \|+ \|\|\|\| \|+\|\|\| \|\| \|\|\|\|#\|+\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 339 TSSQDKAFDSDSEPDAVPYQEGPRTLDQLD#AVSSLPTPSDILVSYSTFP 387
Monodelphis domestica	Query 220 PEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\|\|+\|\|\|\|+\|\| \|\|\|\|\|\| \|\|\| \|#\|++\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 298 PEDKSPGSDPETDATPFQNN---FDQPD#AVASLPTPSDILVSYSTFP 341
Mus musculus	Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\| + + \|+ \|\|\|\| \|+\|\|\| \| \|\|\|\|#\|+\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 339 TSSQGRTLDSDSEPDAVPYQEGPRPLDQLD#AVSSLPTPSDILVSYSTFP 387
Gallus gallus	Query 219 SPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\|+\|\|+ + \| \|\| \|\|\| \|+ \|#\|++\|\|\|\|\| \|\| \|\|\|\|\|\|\| Sbjct 289 SPQDETCRRSIESDAIPFQAPSGNEDEPD#AVASLPTPGDILVSYSTFP 336
Oryctolagus cuniculus	Query 218 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDI 258 \|\| + + \|+ \|\|\|\| \| \|\|\| \| +\|\|\|#\|+\|\|\|\|\|\|\|\|\| Sbjct 175 TSSQGRTLDSDSEPDAVPHQEGPRPLNQLD#AVSSLPTPSDI 215
Dicentrarchus labrax	Query 219 SPEDESP---GSNPEPDATPFQ---EGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\|++ \| \|++ + \|\| \| + \| \| \|+\|\|#\| +\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 309 SPDEVEPSIGGADDQTDAIPTSSSSDSLSTSDELD#ARASLPTPSDILVSYSTFP 362
Xenopus laevis	Query 223 ESPGSNP-----EPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|+\| +\| + \|\|\|\| \| \|++\|#\|+\|++\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 284 ETPPLSPTSTSLQSDATPVFSGEGDRDEVD#AVSNIPTPSDILVSYSTFP 332
Danio rerio	Query 219 SPEDESP---GSNPEPDATPFQEGLRTF----DQLD#AISSLPTPSDIFVSYSTFP 266 \|\|+\| \| \| + \| \|\| \| + \|+\|\|#\| +\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 310 SPDDVQPCIGGIDDEMDAIPMSSSSDSLSTASDELD#ARASLPTPSDILVSYSTFP 364
Oncorhynchus mykiss	Query 219 SPEDESP---GSNPEPDATPFQEGLRTF----DQLD#AISSLPTPSDIFVSYSTFP 266 +\|+++ \| \|++ + \|\| \| + \|+ \|#\| ++\|\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 309 TPDEDRPCIGGTDDQTDAMPMSSSSDSLSTPSDEPD#ARATLPTPSDILVSYSTFP 363
Tetraodon nigroviridis	Query 219 SPEDESP---GSNPEPDATPFQ---EGLRTFDQLD#AISSLPTPSDIFVSYSTFP 266 \|\|++ \| \|++ + \|\| \| + \| \|+ \|#\| +\|\|\|\| \|\|\| \|\|\|\|\|\|\| Sbjct 295 SPDEFEPPVVGADDQTDAIPVSSSSDSLSMSDEPD#ARASLPTSSDILVSYSTFP 348
Aspergillus clavatus NRRL 1	Query 219 SPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYS 263 +\|+\|+\|\| \| \|\|\|\|+ + \| + # + \|+\| \|+\|\| \| \| Sbjct 108 APQDQSPHSEYEPDASTPGSRRQAFGRFR#GVLSIPERSNIFGSGS 152

[Site 2] LTPVVLRPEI³²-RKPEVLRPET

Ile³² Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Leu²³	Thr²⁴	Pro²⁵	Val²⁶	Val²⁷	Leu²⁸	Arg²⁹	Pro³⁰	Glu³¹	Ile³²

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg³³	Lys³⁴	Pro³⁵	Glu³⁶	Val³⁷	Leu³⁸	Arg³⁹	Pro⁴⁰	Glu⁴¹	Thr⁴²

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
ASFLRTNRQAAKLSKPTLENLTPVVLRPEIRKPEVLRPETPRPVDIGSGGFGDVGALESL

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	120.00	10	caspase 9, apoptosis-related cysteine peptidase
2	Pan troglodytes	120.00	4	PREDICTED: caspase 9 isoform 2
3	synthetic construct	120.00	1	Homo sapiens caspase 9, apoptosis-related cysteine
4	N/A	119.00	1	G
5	Macaca fascicularis	113.00	2	caspase 9
6	Macaca mulatta	113.00	1	PREDICTED: similar to caspase 9 isoform alpha prep
7	Mus musculus	59.30	8	caspase 9
8	Rattus norvegicus	53.90	5	25 kDa caspase-9 dominant negative protein
9	Canis familiaris	48.90	2	PREDICTED: caspase 9, apoptosis-related cysteine p
10	Canis lupus familiaris	45.80	1	caspase 9
11	Monodelphis domestica	40.40	1	PREDICTED: similar to ICE-LAP6
12	Chromobacterium violaceum ATCC 12472	38.90	1	hypothetical protein CV2832
13	Bacillus weihenstephanensis KBAB4	33.50	1	Extensin-like protein:NEAr transporter

Top-ranked sequences

organism	matching
Homo sapiens	Query 3 ASFLRTNRQAAKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGFGDVGALESL 62 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 3 ASFLRTNRQAAKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGFGDVGALESL 62
Pan troglodytes	Query 3 ASFLRTNRQAAKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGFGDVGALESL 62 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 3 ASFLRTNRQAAKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGFGDVGALESL 62
synthetic construct	Query 3 ASFLRTNRQAAKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGFGDVGALESL 62 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 3 ASFLRTNRQAAKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGFGDVGALESL 62
N/A	Query 3 ASFLRTNRQAAKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGFGDVGALESL 62 \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 86 ASFLRTNRQAGKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGFGDVGALESL 145
Macaca fascicularis	Query 3 ASFLRTNRQAAKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGFGDVGALESL 62 \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| \|\|\| Sbjct 72 ASFLRTNRQAVKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGCGDVGAPESL 131
Macaca mulatta	Query 3 ASFLRTNRQAAKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGFGDVGALESL 62 \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| \|\|\| Sbjct 86 ASFLRTNRQAVKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGCGDVGAPESL 145
Mus musculus	Query 16 SKPTLENLTPVVLRPEI#R-----KPEVLRPETPRPVDIGSGGFGDV 56 \|+\| + \|\|\|\|\|\|\| \|\| # \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\| Sbjct 131 SQPAVGNLTPVVLGPEE#LWPARLKPEVLRPETPRPVDIGSGGAHDV 176
Rattus norvegicus	Query 16 SKPTLENLTPVVLRPEI#R-----KPEVLRPETPRPVDIGSGGFGDV 56 \|+\| \| \|\|\|\|\|\|\| \|\| # +\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\| Sbjct 132 SQPALGNLTPVVLGPEE#LWPTRLRPEVLTPETPRPVDIGSGRAHDV 177
Canis familiaris	Query 3 ASFLRTNRQAAKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGFGDV 56 \|\| \| +\|\|\| \|\| \| \| \|\|\|\| \| # +\|+\|+\|\| \|\|\| \| \|\|\| \| \|\| Sbjct 86 ASCLTMSRQADP-SKLTPGKLAPVVLGPAE#LQPQVVRPSVPRPTDNGSGRFSDV 138
Canis lupus familiaris	Query 16 SKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGFGDV 56 \|\| \| \| \|\|\|\| \| # +\|+\|+\|\| \|\|\| \| \|\|\| \| \|\| Sbjct 129 SKLTPGKLAPVVLGPAE#LQPQVVRPSVPRPTDNGSGRFSDV 169
Monodelphis domestica	Query 3 ASFLRTNRQAAKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRPVDIGSGGFGDVGALES 61 \|+ \| \|\|+\| \| +\| \| \|\| \| \| \|\| # +\| +\|+ \| \| \| \|\| +\| \| \| Sbjct 84 AALLSENREAGKCLRPHL-NLGPPVSRPLC#DPEQVTKPQLPSPYHSGGGGCIFIGPLRS 141
Chromobacterium violaceum ATCC 12472	Query 13 AKLSKPTLENLTPVVLRPEI#RKPEVL-RPETPRPVDIGSGGFGDVG 57 \| \|\|\|\| \|+\|+ + \|\|\| #+ \|+\| \| \|+\| + \| \|\| +\| Sbjct 111 AALSKPQLQNVFMAIERPET#KPGEILPGEEVPQPTPVNPGEFGSIG 156
Bacillus weihenstephanensis KBAB4	Query 9 NRQAAKLSKPTLENLTPVVLRPEI#RKPEVLRPETPRP 45 \| + ++ \|\| +\| \| \| +\|\|+# \|\|\|\| +\|\| +\| Sbjct 457 NEEKPEVEKPEVEK--PEVEKPEV#EKPEVEKPEVEKP 491

* References

None.