XSB0683 : insulin-like growth factor binding protein 5 [Mus musculus]
[ CaMP Format ]
This entry is computationally expanded from SB0023
* Basic Information
| Organism | Mus musculus (house mouse) |
| Protein Names | Insulin-like growth factor-binding protein 5; IGF-binding protein 5; IGFBP-5; IBP-5; insulin-like growth factor binding protein 5 |
| Gene Names | Igfbp5; Igfbp-5; insulin-like growth factor binding protein 5 |
| Gene Locus | 1 36.1 cM; chromosome 1 |
| GO Function | Not available |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 271 aa
Average Mass: 30.372 kDa
Monoisotopic Mass: 30.352 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| IGFBP 1. | 26 | 84 | 102.7 | 1.3e-27 |
| --- cleavage 160 --- | ||||
| --- cleavage 171 --- | ||||
| Thyroglobulin_1 1. | 191 | 262 | 130.3 | 6.3e-36 |
3. Sequence Information
Fasta Sequence: XSB0683.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
2 [sites]
Cleavage sites (±10aa)
[Site 1] VKKDRRKKLT160-QSKFVGGAEN
Thr160 
Gln
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Val151 | Lys152 | Lys153 | Asp154 | Arg155 | Arg156 | Lys157 | Lys158 | Leu159 | Thr160 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Gln161 | Ser162 | Lys163 | Phe164 | Val165 | Gly166 | Gly167 | Ala168 | Glu169 | Asn170 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| YSPKVFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVIPAPEMRQESEQGP |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Mus musculus | 124.00 | 3 | insulin-like growth factor binding protein 5 |
| 2 | Rattus norvegicus | 122.00 | 1 | insulin-like growth factor binding protein 5 |
| 3 | Macaca mulatta | 120.00 | 3 | PREDICTED: similar to insulin-like growth factor b |
| 4 | Homo sapiens | 120.00 | 2 | IGFBP5 |
| 5 | Canis familiaris | 120.00 | 1 | PREDICTED: similar to insulin-like growth factor b |
| 6 | synthetic construct | 120.00 | 1 | insulin-like growth factor binding protein 5 |
| 7 | Sus scrofa | 119.00 | 2 | insulin-like growth factor binding protein 5 |
| 8 | Bos taurus | 119.00 | 2 | PREDICTED: similar to Insulin-like growth factor-b |
| 9 | N/A | 119.00 | 1 | 2 |
| 10 | Monodelphis domestica | 104.00 | 2 | PREDICTED: similar to insulin-like growth factor b |
| 11 | Gallus gallus | 90.50 | 2 | PREDICTED: similar to insulin-like growth factor b |
| 12 | Coturnix japonica | 90.50 | 1 | AF293839_1 insulin-like growth factor binding prot |
| 13 | Oryza sativa (japonica cultivar-group) | 34.30 | 1 | retrotransposon protein, putative, Ty3-gypsy sub-c |
| 14 | Equus caballus | 33.90 | 1 | insulin-like growth factor binding protein-5 |
Top-ranked sequences
| organism | matching |
|---|---|
| Mus musculus | Query 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 |
| Rattus norvegicus | Query 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 ||||||||||||||||||||||||||||||#||||||||||||||||||||||||||+||| Sbjct 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESDQGP 190 |
| Macaca mulatta | Query 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 ||||+|||||||||||||||||||||||||#||||||||||||||||| |||||||||||| Sbjct 132 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVISAPEMRQESEQGP 191 |
| Homo sapiens | Query 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 ||||+|||||||||||||||||||||||||#|||||||||||||||+| |||||||||||| Sbjct 132 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRIISAPEMRQESEQGP 191 |
| Canis familiaris | Query 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 ||||+|||||||||||||||||||||||||#||||||||||||||||| |||||||||||| Sbjct 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVISAPEMRQESEQGP 190 |
| synthetic construct | Query 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 ||||+|||||||||||||||||||||||||#|||||||||||||||+| |||||||||||| Sbjct 132 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRIISAPEMRQESEQGP 191 |
| Sus scrofa | Query 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 ||||+|||||||||||||||||||||||||#||||||||||||||||| |||||||||||| Sbjct 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190 |
| Bos taurus | Query 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 ||||+|||||||||||||||||||||||||#||||||||||||||||| ||||||+||||| Sbjct 136 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVISAPEMRQKSEQGP 195 |
| N/A | Query 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 ||||+|||||||||||||||||||||||||#||||||||||||||||| |||||||||||| Sbjct 131 YSPKIFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVILAPEMRQESEQGP 190 |
| Monodelphis domestica | Query 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 |||||||||| +||+||||+|||||||||#|||||| ||||| ||| ||||||||||| Sbjct 131 YSPKVFRPKHVHLSEMKAEALKKDRRKKLT#QSKFVGAAENTAQSRVISVPEMRQESEQGP 190 |
| Gallus gallus | Query 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 | || +|||| |+|+|||||+|||||||||# +|||| |||||||||+ ||+||| | || Sbjct 45 YPPKAYRPKHGRLSDLKAEALKKDRRKKLT#LAKFVGMAENTAHPRVV-IPELRQEFELGP 103 |
| Coturnix japonica | Query 131 YSPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 | || +|||| |+|+|||||+|||||||||# +|||| |||||||||+ ||+||| | || Sbjct 88 YPPKAYRPKHGRLSDLKAEALKKDRRKKLT#LAKFVGMAENTAHPRVV-IPELRQEFELGP 146 |
| Oryza sativa (japonica cultivar-group) | Query 132 SPKVFRPKHTRISELKAEAVKKDRRKKLT#QSKFVGGAENTAHPRVIPAPEMRQESEQGP 190 | | | |+ + | |+ ||++| # || | + +| ||+ |+| +| + | | Sbjct 239 SMKTLVDKALRVESSEKEVVE-DRKRKWA#AKKFAGSSSST-RPRLAPSPAVRPMAPQPP 295 |
| Equus caballus | Query 131 YSPKVFRPKHTRIS 144 |||||||||||||| Query 131 YSPKVFRPKHTRIS 144 |
[Site 2] SKFVGGAENT171-AHPRVIPAPE
Thr171 Ala
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Ser162 | Lys163 | Phe164 | Val165 | Gly166 | Gly167 | Ala168 | Glu169 | Asn170 | Thr171 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ala172 | His173 | Pro174 | Arg175 | Val176 | Ile177 | Pro178 | Ala179 | Pro180 | Glu181 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| RISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVIPAPEMRQESEQGPCRRHMEASLQE |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Mus musculus | 122.00 | 5 | insulin-like growth factor binding protein 5 |
| 2 | Rattus norvegicus | 121.00 | 1 | insulin-like growth factor binding protein 5 |
| 3 | Canis familiaris | 119.00 | 3 | PREDICTED: similar to insulin-like growth factor b |
| 4 | Macaca mulatta | 119.00 | 3 | PREDICTED: similar to insulin-like growth factor b |
| 5 | Homo sapiens | 119.00 | 2 | insulin-like growth factor binding protein 5 |
| 6 | synthetic construct | 119.00 | 1 | insulin-like growth factor binding protein 5 |
| 7 | Sus scrofa | 118.00 | 2 | insulin-like growth factor binding protein 5 |
| 8 | N/A | 118.00 | 1 | 2 |
| 9 | Bos taurus | 117.00 | 1 | PREDICTED: similar to Insulin-like growth factor-b |
| 10 | Monodelphis domestica | 104.00 | 2 | PREDICTED: similar to insulin-like growth factor b |
| 11 | Gallus gallus | 96.30 | 2 | PREDICTED: similar to insulin-like growth factor b |
| 12 | Coturnix japonica | 96.30 | 1 | AF293839_1 insulin-like growth factor binding prot |
| 13 | Xenopus laevis | 38.90 | 1 | insulin-like growth factor binding protein 5 |
| 14 | Xenopus tropicalis | 38.10 | 1 | Unknown (protein for MGC:144994) |
| 15 | Salmo salar | 33.50 | 2 | IGF binding protein 5 |
| 16 | Oncorhynchus mykiss | 33.50 | 1 | insulin-like growth factor binding protein 5 |
| 17 | Oryza sativa (japonica cultivar-group) | 32.70 | 2 | retrotransposon protein, putative, Ty3-gypsy sub-c |
| 18 | Danio rerio | 32.70 | 2 | PREDICTED: similar to Igfbp5 protein |
| 19 | Aquifex pyrophilus | 32.30 | 1 | NAD-dependent DNA ligase |
| 20 | Tetraodon nigroviridis | 32.30 | 1 | unnamed protein product |
| 21 | Oreochromis mossambicus | 32.30 | 1 | AF406954_1 insulin-like growth factor binding prot |
Top-ranked sequences
| organism | matching |
|---|---|
| Mus musculus | Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 |
| Rattus norvegicus | Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 ||||||||||||||||||||||||||||||#|||||||||||||||+|||||||||||||| Sbjct 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESDQGPCRRHMEASLQE 201 |
| Canis familiaris | Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 ||||||||||||||||||||||||||||||#|||||| ||||||||||||||||||||||| Sbjct 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVISAPEMRQESEQGPCRRHMEASLQE 201 |
| Macaca mulatta | Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 ||||||||||||||||||||||||||||||#|||||| ||||||||||||||||||||||| Sbjct 143 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVISAPEMRQESEQGPCRRHMEASLQE 202 |
| Homo sapiens | Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 ||||||||||||||||||||||||||||||#||||+| ||||||||||||||||||||||| Sbjct 143 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRIISAPEMRQESEQGPCRRHMEASLQE 202 |
| synthetic construct | Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 ||||||||||||||||||||||||||||||#||||+| ||||||||||||||||||||||| Sbjct 143 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRIISAPEMRQESEQGPCRRHMEASLQE 202 |
| Sus scrofa | Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 ||||||||||||||||||||||||||||||#|||||| ||||||||||||||||||||||| Sbjct 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201 |
| N/A | Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 ||||||||||||||||||||||||||||||#|||||| ||||||||||||||||||||||| Sbjct 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVILAPEMRQESEQGPCRRHMEASLQE 201 |
| Bos taurus | Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 ||||||||||||||||||||||||||||||#|||||| ||||||+|||||||||||||||| Sbjct 147 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVISAPEMRQKSEQGPCRRHMEASLQE 206 |
| Monodelphis domestica | Query 143 ISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 +||+||||+||||||||||||||| ||||#| ||| |||||||||||||||||||||| Sbjct 143 LSEMKAEALKKDRRKKLTQSKFVGAAENT#AQSRVISVPEMRQESEQGPCRRHMEASLQE 201 |
| Gallus gallus | Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 |+|+|||||+||||||||| +|||| ||||#|||||+ ||+||| | ||||||||||||| Sbjct 56 RLSDLKAEALKKDRRKKLTLAKFVGMAENT#AHPRVV-IPELRQEFELGPCRRHMEASLQE 114 |
| Coturnix japonica | Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 |+|+|||||+||||||||| +|||| ||||#|||||+ ||+||| | ||||||||||||| Sbjct 99 RLSDLKAEALKKDRRKKLTLAKFVGMAENT#AHPRVV-IPELRQEFELGPCRRHMEASLQE 157 |
| Xenopus laevis | Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 |+|+ || |+ |+|| +||+ | | # | || | + ||||| +| +|| Sbjct 140 RLSDQKAIALNTFRQKKHSQSRIVS-VEKV#QSPST---PEHSIEIDMGPCRRQVETLMQE 195 |
| Xenopus tropicalis | Query 142 RISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQE 201 |+++ || |+ |+|| +||+ | | # | || | + ||||| +| +|| Sbjct 136 RLTDQKAIALNTFRQKKQSQSRIVS-VEKV#QSPST---PEHSIEIDMGPCRRQVETLMQE 191 |
| Salmo salar | Query 143 ISELKAEAVKKDRRKKLTQSKF--VGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQ 200 |+ | +|++||+ +| |+| +| + +# | | + | | ||||| ++ +| Sbjct 145 INSKKIQAMRKDKDRKRAQAKLRSIGPMDYS#------PLPIDKHEPEFGPCRRKLDGIIQ 198 |
| Oncorhynchus mykiss | Query 143 ISELKAEAVKKDRRKKLTQSKF--VGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQ 200 |+ | +|++||+ +| |+| +| + +# | | + | | ||||| ++ +| Sbjct 145 INSKKIQAMRKDKDRKRAQAKLRSIGPMDYS#------PLPIDKHEPEFGPCRRKLDGIIQ 198 |
| Oryza sativa (japonica cultivar-group) | Query 145 ELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGP 190 | + | +||++| || | + +|# ||+ |+| +| + | | Sbjct 251 ESSEKEVVEDRKRKWAAKKFAGSSSST#-RPRLAPSPAVRPMAPQPP 295 |
| Danio rerio | Query 143 ISELKAEAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASLQ 200 |+ | |+ ||++|+ + + || + +# | | + | | ||||| ++ +| Sbjct 141 INSKKQAALFKDKKKQQEKLRSVGSLDYS#------PLPIDKHEPEFGPCRRKLDGIIQ 192 |
| Aquifex pyrophilus | Query 142 RISELKAEAV-KKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEA 197 +++|++ | | |+ ||| | + | #|+|| | +||+ + +| +|| Sbjct 194 KLAEIRGEVVINKEEFKKLNQERIEEGLPPF#ANPRNAAAGSIRQKDPKEVAKRRLEA 250 |
| Tetraodon nigroviridis | Query 149 EAVKKDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASL 199 + ++||+ || | + |# + | ++||| ||||| ||+ | Sbjct 152 QLLQKDQNKKTQSYKVEAVSRRT#ITDVQNFSLENKRESEYGPCRREMESIL 202 |
| Oreochromis mossambicus | Query 147 KAEAVK--KDRRKKLTQSKFVGGAENT#AHPRVIPAPEMRQESEQGPCRRHMEASL 199 ||| +| +| | + ++ | + || |# + +||+| ||||| ||+ | Sbjct 119 KAEVIKNAEDNRSQRSKVKPLPGAVGT#DGQNF--SCGSKQETENGPCRREMESVL 171 |
* References
[PubMed ID: 19236847] Beattie J, Kreiner M, Allan GJ, Flint DJ, Domingues D, van der Walle CF, IGFBP-3 and IGFBP-5 associate with the cell binding domain (CBD) of fibronectin. Biochem Biophys Res Commun. 2009 Apr 17;381(4):572-6. Epub 2009 Feb 21.
[PubMed ID: 18762576] ... Ren H, Yin P, Duan C, IGFBP-5 regulates muscle cell differentiation by binding to IGF-II and switching on the IGF-II auto-regulation loop. J Cell Biol. 2008 Sep 8;182(5):979-91. Epub 2008 Sep 1.
[PubMed ID: 18725516] ... Kawaguchi A, Ikawa T, Kasukawa T, Ueda HR, Kurimoto K, Saitou M, Matsuzaki F, Single-cell gene profiling defines differential progenitor subclasses in mammalian neurogenesis. Development. 2008 Sep;135(18):3113-24.
[PubMed ID: 18973680] ... Tamplin OJ, Kinzel D, Cox BJ, Bell CE, Rossant J, Lickert H, Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives. BMC Genomics. 2008 Oct 30;9:511.
[PubMed ID: 18554416] ... Hoffman BG, Zavaglia B, Witzsche J, Ruiz de Algara T, Beach M, Hoodless PA, Jones SJ, Marra MA, Helgason CD, Identification of transcripts with enriched expression in the developing and adult pancreas. Genome Biol. 2008;9(6):R99. Epub 2008 Jun 14.
[PubMed ID: 7533502] ... Kou K, Mittanck DW, Fu C, Rotwein P, Structure and function of the mouse insulin-like growth factor binding protein 5 gene promoter. DNA Cell Biol. 1995 Mar;14(3):241-9.
[PubMed ID: 7529732] ... Schuller AG, Groffen C, van Neck JW, Zwarthoff EC, Drop SL, cDNA cloning and mRNA expression of the six mouse insulin-like growth factor binding proteins. Mol Cell Endocrinol. 1994 Aug;104(1):57-66.
[PubMed ID: 7525452] ... Kou K, James PL, Clemmons DR, Copeland NG, Gilbert DJ, Jenkins NA, Rotwein P, Identification of two clusters of mouse insulin-like growth factor binding protein genes on chromosomes 1 and 11. Genomics. 1994 Jun;21(3):653-5.
[PubMed ID: 7518410] ... Kou K, Jenkins NA, Gilbert DJ, Copeland NG, Rotwein P, Organization, expression, and chromosomal location of the mouse insulin-like growth factor binding protein 5 gene. Genomics. 1994 Apr;20(3):412-8.
[PubMed ID: 7511095] ... Hahnel A, Schultz GA, Insulin-like growth factor binding proteins are transcribed by preimplantation mouse embryos. Endocrinology. 1994 Apr;134(4):1956-9.