XSB1031 : Chain A, Crystal Structure Of A Dimeric Caspase-9
[ CaMP Format ]
This entry is computationally expanded from SB0042
* Basic Information
| Organism | Homo sapiens (human) |
| Protein Names | Caspase-9; CASP-9; 3.4.22.62; ICE-like apoptotic protease 6; ICE-LAP6; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; Caspase-9 subunit p35; Caspase-9 subunit p10 |
| Gene Names | MCH6 |
| Gene Locus | Not available |
| GO Function | Not available |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 278 aa
Average Mass: 30.521 kDa
Monoisotopic Mass: 30.501 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| Peptidase_C14 1. | 23 | 275 | 308.7 | 1.2e-89 |
| --- cleavage 192 (inside Peptidase_C14 23..275) --- | ||||
3. Sequence Information
Fasta Sequence: XSB1031.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
* Cleavage Information
1 [sites]
Cleavage sites (±10aa)
[Site 1] EGLRTFDQLD192-AISSLPTPSD
Asp192 
Ala
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Glu183 | Gly184 | Leu185 | Arg186 | Thr187 | Phe188 | Asp189 | Gln190 | Leu191 | Asp192 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ala193 | Ile194 | Ser195 | Ser196 | Leu197 | Pro198 | Thr199 | Pro200 | Ser201 | Asp202 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| TSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSG |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | N/A | 129.00 | 29 | A |
| 2 | Homo sapiens | 129.00 | 13 | caspase-9 |
| 3 | Pan troglodytes | 129.00 | 6 | PREDICTED: caspase 9 isoform 2 |
| 4 | synthetic construct | 129.00 | 6 | Homo sapiens caspase 9, apoptosis-related cysteine |
| 5 | Macaca mulatta | 117.00 | 6 | PREDICTED: similar to caspase 9 isoform alpha prep |
| 6 | Macaca fascicularis | 117.00 | 2 | caspase 9 |
| 7 | Felis catus | 107.00 | 3 | CASP9 |
| 8 | Canis familiaris | 105.00 | 3 | PREDICTED: caspase 9, apoptosis-related cysteine p |
| 9 | Canis lupus familiaris | 105.00 | 2 | caspase 9 |
| 10 | Monodelphis domestica | 97.40 | 3 | PREDICTED: similar to ICE-LAP6 |
| 11 | Rattus norvegicus | 96.30 | 5 | caspase 9 |
| 12 | Mus musculus | 91.30 | 12 | unnamed protein product |
| 13 | Gallus gallus | 73.90 | 2 | caspase 9 |
| 14 | Dicentrarchus labrax | 69.70 | 2 | caspase-9 |
| 15 | Xenopus laevis | 66.20 | 6 | caspase-9 |
| 16 | Danio rerio | 65.90 | 4 | Zgc:101776 protein |
| 17 | Tetraodon nigroviridis | 65.10 | 2 | unnamed protein product |
| 18 | Oncorhynchus mykiss | 60.80 | 1 | caspase-9 |
| 19 | Oryctolagus cuniculus | 52.00 | 3 | caspase 9 |
| 20 | Tribolium castaneum | 40.40 | 1 | PREDICTED: similar to Caspase precursor (drICE), p |
| 21 | Branchiostoma floridae | 40.00 | 1 | AF412335_1 amphiCASP-3/7 |
| 22 | Schistosoma japonicum | 39.30 | 4 | SJCHGC04214 protein |
| 23 | Anopheles gambiae str. PEST | 38.50 | 10 | ENSANGP00000008707 |
| 24 | Aedes aegypti | 38.10 | 2 | caspase-1 |
| 25 | Strongylocentrotus purpuratus | 37.70 | 4 | PREDICTED: similar to caspase-3, partial |
| 26 | Xenopus tropicalis | 37.70 | 2 | caspase 7, apoptosis-related cysteine peptidase |
| 27 | Contains: Caspase-3 p17 subunit; Caspase-3 p12 subunit | 36.60 | 3 | CASP3_CRILO Caspase-3 precursor (CASP-3) (Apopain) |
| 28 | Bos taurus | 35.80 | 4 | PREDICTED: similar to Mch3 isoform alpha |
| 29 | Geodia cydonium | 35.80 | 2 | caspase 3 |
| 30 | Equus caballus | 35.40 | 3 | caspase-3 |
| 31 | Gobius niger | 35.00 | 1 | caspase 3 |
| 32 | Sus scrofa | 34.70 | 1 | caspase 3 |
| 33 | Cricetulus griseus | 34.30 | 1 | caspase 3 |
| 34 | Contains: Caspase-7 subunit p20; Caspase-7 subunit p11 | 34.30 | 1 | CASP7_MESAU Caspase-7 precursor (CASP-7) (ICE-like |
| 35 | Salmo salar | 33.90 | 2 | caspase 7 |
| 36 | Ovis aries | 33.90 | 1 | caspase-3 |
| 37 | Drosophila melanogaster | 33.50 | 3 | drICE protein |
| 38 | Drosophila pseudoobscura | 33.50 | 2 | GA20588-PA |
| 39 | Apis mellifera | 33.50 | 1 | PREDICTED: similar to Caspase precursor (drICE) |
| 40 | Chrysiptera parasema | 33.10 | 1 | caspase 3 |
| 41 | Oryzias latipes | 33.10 | 1 | caspase 3A |
| 42 | Aspergillus clavatus NRRL 1 | 32.70 | 1 | pentatricopeptide repeat protein |
| 43 | Takifugu rubripes | 32.30 | 2 | caspase 3 |
Top-ranked sequences
| organism | matching |
|---|---|
| N/A | Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 |
| Homo sapiens | Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 |
| Pan troglodytes | Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 |
| synthetic construct | Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 |
| Macaca mulatta | Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 ||||||| ||||||||||||| ||||||||#|+||||||||||||||||||||| |||||| Sbjct 301 TSPEDESSGSNPEPDATPFQEDLRTFDQLD#AVSSLPTPSDIFVSYSTFPGFVSLRDPKSG 360 |
| Macaca fascicularis | Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 ||||||| ||||||||||||| ||||||||#|+||||||||||||||||||||| |||||| Sbjct 287 TSPEDESSGSNPEPDATPFQEDLRTFDQLD#AVSSLPTPSDIFVSYSTFPGFVSLRDPKSG 346 |
| Felis catus | Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 ||||| +|||+||||| |||| |||| |#|+|||||||||||||||||||||||| ||| Sbjct 254 TSPEDRTPGSDPEPDAVAFQEGPGTFDQPD#AVSSLPTPSDIFVSYSTFPGFVSWRDTKSG 313 |
| Canis familiaris | Query 164 SPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 |||| ||||+ |||| ||||| |||||#|+|||||||||||||||||||||||+ ||| Sbjct 301 SPEDRSPGSDSEPDAVPFQEGPGPFDQLD#AVSSLPTPSDIFVSYSTFPGFVSWRNTKSG 359 |
| Canis lupus familiaris | Query 164 SPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 |||| ||||+ |||| ||||| |||||#|+|||||||||||||||||||||||+ ||| Sbjct 332 SPEDRSPGSDSEPDAVPFQEGPGPFDQLD#AVSSLPTPSDIFVSYSTFPGFVSWRNTKSG 390 |
| Monodelphis domestica | Query 165 PEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 |||+||||+|| |||||| ||| |#|++|||||||| |||||||||||||||||| Sbjct 298 PEDKSPGSDPETDATPFQNN---FDQPD#AVASLPTPSDILVSYSTFPGFVSWRDPKSG 352 |
| Rattus norvegicus | Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 || +|++ |+ |||| |+||| || ||||#|+||||||||| |||||||||||||| ||| Sbjct 339 TSSQDKAFDSDSEPDAVPYQEGPRTLDQLD#AVSSLPTPSDILVSYSTFPGFVSWRDKKSG 398 |
| Mus musculus | Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 || + + |+ |||| |+||| | ||||#|+||||||||| |||||||||||||| ||| Sbjct 338 TSSQGRTLDSDSEPDAVPYQEGPRPLDQLD#AVSSLPTPSDILVSYSTFPGFVSWRDKKSG 397 |
| Gallus gallus | Query 164 SPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 ||+||+ + | || ||| |+ |#|++||||| || |||||||||||||| || Sbjct 289 SPQDETCRRSIESDAIPFQAPSGNEDEPD#AVASLPTPGDILVSYSTFPGFVSWRDKVSG 347 |
| Dicentrarchus labrax | Query 164 SPEDESP---GSNPEPDATPFQ---EGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWR 217 ||++ | |++ + || | + | | |+||#| +|||||||| ||||||||+|||| Sbjct 309 SPDEVEPSIGGADDQTDAIPTSSSSDSLSTSDELD#ARASLPTPSDILVSYSTFPGYVSWR 368 Query 218 DPKSG 222 | +|| Sbjct 369 DTQSG 373 |
| Xenopus laevis | Query 168 ESPGSNP-----EPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 |+| +| + |||| | |++|#|+|++|||||| ||||||||+||||| +| Sbjct 284 ETPPLSPTSTSLQSDATPVFSGEGDRDEVD#AVSNIPTPSDILVSYSTFPGYVSWRDKHTG 343 |
| Danio rerio | Query 164 SPEDESP---GSNPEPDATPFQEGLRTF----DQLD#AISSLPTPSDIFVSYSTFPGFVSW 216 ||+| | | + | || | + |+||#| +|||||||| ||||||||+||| Sbjct 310 SPDDVQPCIGGIDDEMDAIPMSSSSDSLSTASDELD#ARASLPTPSDILVSYSTFPGYVSW 369 Query 217 RDPKSG 222 || ++| Sbjct 370 RDTEAG 375 |
| Tetraodon nigroviridis | Query 164 SPEDESP---GSNPEPDATPFQ---EGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWR 217 ||++ | |++ + || | + | |+ |#| +|||| ||| ||||||||+|||| Sbjct 295 SPDEFEPPVVGADDQTDAIPVSSSSDSLSMSDEPD#ARASLPTSSDILVSYSTFPGYVSWR 354 Query 218 DPKSG 222 |||+| Sbjct 355 DPKAG 359 |
| Oncorhynchus mykiss | Query 164 SPEDESP---GSNPEPDATPFQEGLRTF----DQLD#AISSLPTPSDIFVSYSTFPGFVSW 216 +|+++ | |++ + || | + |+ |#| ++||||||| ||||||||+||| Sbjct 309 TPDEDRPCIGGTDDQTDAMPMSSSSDSLSTPSDEPD#ARATLPTPSDILVSYSTFPGYVSW 368 Query 217 RDPKSG 222 || ++| Sbjct 369 RDTQAG 374 |
| Oryctolagus cuniculus | Query 163 TSPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDI 203 || + + |+ |||| | ||| | +|||#|+||||||||| Sbjct 175 TSSQGRTLDSDSEPDAVPHQEGPRPLNQLD#AVSSLPTPSDI 215 |
| Tribolium castaneum | Query 192 D#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 |# ++| +|| | ||| || ||||||+| Sbjct 81 D#VSYTIPIMADILVMYSTVEGFYSWRDPKNG 111 |
| Branchiostoma floridae | Query 176 PDATPFQEGLRTFDQLD#A--ISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 ||| | + |+||#| ++|| +| ++||| ||+ |||+| | Sbjct 217 PDALPEVQ-----DELD#AGNKATLPAEADFLLAYSTVPGYYSWRNPGRG 260 |
| Schistosoma japonicum | Query 194 ISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 + || +|| ||||| ||+++||+ || Sbjct 183 VHKLPVEADILVSYSTVPGYIAWRNMTSG 211 |
| Anopheles gambiae str. PEST | Query 179 TPFQEGLR----TFDQLD#AISS-------LPTPSDIFVSYSTFPGFVSWRDPKSG 222 | | +|++ | +|#|+|| +|| +|+ | || | | |||+| | Sbjct 129 TKFDKGVKLTKIATDTVD#ALSSSSQRTCVIPTMADVLVMYSAFDGHYSWRNPTHG 183 |
| Aedes aegypti | Query 190 QLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 | # + |+| +|+ | |||+ |+ |||+|+ | Sbjct 184 QEQ#VLYSIPAMADLLVMYSTYDGYYSWRNPRQG 216 |
| Strongylocentrotus purpuratus | Query 195 SSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 | +|+ ||+ ++|+| |||||||+ + | Sbjct 191 SKVPSQSDMLLAYATVPGFVSWRNSERG 218 |
| Xenopus tropicalis | Query 175 EPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 | || | + | | +# +| +| +||| ||+ |||+| | Sbjct 206 ETDAGPSNDSLET--DAN#PRHKIPVEADFLYAYSTVPGYYSWRNPGRG 251 |
| Contains: Caspase-3 p17 subunit; Caspase-3 p12 subunit | Query 197 LPTPSDIFVSYSTFPGFVSWRDPKSG 222 +| +| +||| ||+ |||+|| | Sbjct 187 IPVEADFLYAYSTAPGYYSWRNPKDG 212 |
| Bos taurus | Query 197 LPTPSDIFVSYSTFPGFVSWRDPKSG 222 +| +| +||| ||+ |||+| || Sbjct 240 IPVEADFLFAYSTVPGYYSWRNPGSG 265 |
| Geodia cydonium | Query 196 SLPTPSDIFVSYSTFPGFVSWRDPKSG 222 +||| +| ++|+| ||+||||+ + | Sbjct 221 ALPTEADFVLAYATVPGYVSWRNSEYG 247 |
| Equus caballus | Query 179 TPFQEGLRTFDQLD---#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 | |+ | ++ #| +| +| +||| ||+ |||+ | | Sbjct 63 TELDSGIETDSGIEDDM#ACQKIPVEADFLYAYSTAPGYYSWRNSKDG 109 |
| Gobius niger | Query 179 TPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 | |+ | |# + +| +| +||| ||+ |||+ +| Sbjct 118 TDLDAGIETDSPDD#EGARIPVEADFLYAYSTAPGYYSWRNTMTG 161 |
| Sus scrofa | Query 193 AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 | +| +| +||| ||+ |||+ | | Sbjct 183 ACQKIPVEADFLYAYSTAPGYYSWRNSKDG 212 |
| Cricetulus griseus | Query 193 AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 | +| +| +||| ||+ |||+ | | Sbjct 183 ACHKIPVEADFLYAYSTAPGYYSWRNSKDG 212 |
| Contains: Caspase-7 subunit p20; Caspase-7 subunit p11 | Query 197 LPTPSDIFVSYSTFPGFVSWRDPKSG 222 +| +| +||| ||+ |||+| | Sbjct 213 IPVEADFLFAYSTVPGYYSWRNPGKG 238 |
| Salmo salar | Query 197 LPTPSDIFVSYSTFPGFVSWRDPKSG 222 +| +| +||| ||+ |||+| | Sbjct 155 IPVEADFLFAYSTVPGYYSWRNPGRG 180 |
| Ovis aries | Query 193 AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 | +| +| +||| ||+ |||+ | | Sbjct 152 ACQKIPVEADFLYAYSTAPGYFSWRNSKYG 181 |
| Drosophila melanogaster | Query 197 LPTPSDIFVSYSTFPGFVSWRDPKSG 222 +| +| ++||| ||| |||+ | Sbjct 239 IPVHADFLIAYSTVPGFYSWRNTTRG 264 |
| Drosophila pseudoobscura | Query 197 LPTPSDIFVSYSTFPGFVSWRDPKSG 222 +| +| ++||| ||| |||+ | Sbjct 236 IPVHADFLIAYSTVPGFYSWRNTTRG 261 |
| Apis mellifera | Query 182 QEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 +| | | #+ +|+ +| ++||| ||+ |||+ | Sbjct 186 KERTETDGQPA#STFRIPSHADFLIAYSTIPGYYSWRNTTRG 226 |
| Chrysiptera parasema | Query 179 TPFQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 | |+ | | +# + +| +| ++|| ||+ |||+ +| Sbjct 111 TDLDAGIET-DSGE#GTTKIPVEADFLYAFSTAPGYYSWRNTMTG 153 |
| Oryzias latipes | Query 181 FQEGLRTFDQLD#AISSLPTPSDIFVSYSTFPGFVSWRDPKSG 222 | ||+ + |# +| +| +||| |+ ||| +| Sbjct 181 FDEGIEHDAKDD#TSEKIPLEADFLFAYSTVSGYYSWRSTANG 222 |
| Aspergillus clavatus NRRL 1 | Query 164 SPEDESPGSNPEPDATPFQEGLRTFDQLD#AISSLPTPSDIFVSYS 208 +|+|+|| | ||||+ + | + # + |+| |+|| | | Sbjct 108 APQDQSPHSEYEPDASTPGSRRQAFGRFR#GVLSIPERSNIFGSGS 152 |
| Takifugu rubripes | Query 184 GLRTFDQLD#AISS-LPTPSDIFVSYSTFPGFVSWRDPKSG 222 |+ | |#+ ++ +| +| ++|| ||+ |||+ || Sbjct 177 GIETDSAAD#SSTTKIPVEADFLYAFSTAPGYYSWRNTTSG 216 |
* References
[PubMed ID: 15941357] Chao Y, Shiozaki EN, Srinivasula SM, Rigotti DJ, Fairman R, Shi Y, Engineering a dimeric caspase-9: a re-evaluation of the induced proximity model for caspase activation. PLoS Biol. 2005 Jun;3(6):e183. Epub 2005 May 10.