CaMPDB :: Substrate

XSB2962 : plasma membrane calcium ATPase 2 isoform 1 [Mus musculus]

This entry is computationally expanded from SB0056

* Basic Information

Organism	Mus musculus (house mouse)
Protein Names	Plasma membrane calcium-transporting ATPase 2; PMCA2; 3.6.3.8; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2; plasma membrane calcium ATPase 2 isoform 1; wriggle; deaf waddler
Gene Names	Atp2b2; Pmca2; ATPase, Ca++ transporting, plasma membrane 2
Gene Locus	6 49.5 cM; chromosome 6
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_033853	NM_009723	11941	AT2B2_MOUSE	N/A	N/A	N/A	mmu:11941

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 1198 aa

Average Mass: 132.588 kDa

Monoisotopic Mass: 132.503 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
Cation_ATPase_N 1.	41	124	75.0	2.8e-19
E1-E2_ATPase 1.	156	444	225.1	1.8e-64
Hydrolase 1.	448	796	55.6	1.9e-13
Cation_ATPase_C 1.	857	1039	210.7	3.8e-60

3. Sequence Information

Fasta Sequence: XSB2962.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites]

Cleavage sites (±10aa)

[Site 1] EIDHAERELR¹⁰⁷⁹-RGQILWFRGL

Arg¹⁰⁷⁹ Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Glu¹⁰⁷⁰	Ile¹⁰⁷¹	Asp¹⁰⁷²	His¹⁰⁷³	Ala¹⁰⁷⁴	Glu¹⁰⁷⁵	Arg¹⁰⁷⁶	Glu¹⁰⁷⁷	Leu¹⁰⁷⁸	Arg¹⁰⁷⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg¹⁰⁸⁰	Gly¹⁰⁸¹	Gln¹⁰⁸²	Ile¹⁰⁸³	Leu¹⁰⁸⁴	Trp¹⁰⁸⁵	Phe¹⁰⁸⁶	Arg¹⁰⁸⁷	Gly¹⁰⁸⁸	Leu¹⁰⁸⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
AGRLTQKEEIPEEELNEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEG

Summary

#	organism	max score	hits	top seq
1	Canis familiaris	89.00	102	PREDICTED: similar to plasma membrane calcium ATPa
2	N/A	89.00	41	-
3	Homo sapiens	89.00	27	plasma membrane calcium ATPase 2 isoform 2
4	Mus musculus	89.00	12	plasma membrane Ca2+-ATPase isoform 2
5	Bos taurus	89.00	12	PREDICTED: similar to plasma membrane calcium ATPa
6	Pan troglodytes	89.00	11	PREDICTED: plasma membrane calcium ATPase 2 isofor
7	Macaca mulatta	89.00	7	PREDICTED: plasma membrane calcium ATPase 2, parti
8	Rattus norvegicus	89.00	6	ATPase, Ca++ transporting, plasma membrane 2
9	Gallus gallus	87.80	9	PREDICTED: similar to plasma membrane calcium ATPa
10	Tetraodon nigroviridis	85.50	4	unnamed protein product
11	Danio rerio	85.50	4	PREDICTED: hypothetical protein, partial
12	Monodelphis domestica	82.00	3	PREDICTED: similar to plasma membrane calcium ATPa
13	Sus scrofa	82.00	2	plasma membrane calcium ATPase 1
14	Rana catesbeiana	80.10	2	PMCA1bx
15	Oryctolagus cuniculus	73.20	1	Ca2+/Mg2+ ATPase
16	synthetic construct	71.60	2	hypothetical protein
17	Xenopus laevis	70.50	1	ATPase, Ca++ transporting, plasma membrane 3
18	rats, ROS 17/2.8 osteosarcoma cells, Peptide Partial, 123 aa	68.90	1	plasma membrane Ca(2+)-ATPase PMCA1b isoform {alte
19	Pongo pygmaeus	66.60	1	hypothetical protein
20	Procambarus clarkii	66.20	1	plasma membrane calcium ATPase PMCA3

Top-ranked sequences

organism	matching
Canis familiaris	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1050 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109
N/A	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1050 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109
Homo sapiens	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1050 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109
Mus musculus	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1050 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109
Bos taurus	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1050 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109
Pan troglodytes	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1056 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1115
Macaca mulatta	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 542 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 601
Rattus norvegicus	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1050 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109
Gallus gallus	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|+\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1052 AGRLTEKEEVPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1111
Tetraodon nigroviridis	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 288 AGQLTQKDELPEEDVNEENEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 347
Danio rerio	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 293 AGQLTQKDEMPEEEMNEDQEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 352
Monodelphis domestica	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\| \|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1059 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 1118
Sus scrofa	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\| \|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1072 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 1131
Rana catesbeiana	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\| \| \| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1066 AGHGTHKDEIPEEELAEDAEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 1125
Oryctolagus cuniculus	Query 1054 TQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\| \| \|\|\|\|\|\|\|#\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1076 TQKREIGEEELAEDVEEIVHAERELR#RWQILWFRGLNRIQTQIRVVNAFRSSLYEG 1131
synthetic construct	Query 1071 IDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYE 1108 \|\|\|\|\| \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\| \|\|\|+\| Sbjct 1079 IDHAEMELR#RGQILWFRGLNRIQTQIKVVKAFHSSLHE 1116
Xenopus laevis	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRS 1104 \|\| \|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\| \|+\| Sbjct 1098 AGNLTQKEENQEEELNEDAEEIDHAERELR#RGQILWFRGLNRIQTQIEVVNTFKS 1152
rats, ROS 17/2.8 osteosarcoma cells, Peptide Partial, 123 aa	Query 1076 RELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1 RELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 34
Pongo pygmaeus	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRS 1104 \|\| \|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+ \|\| \|\|+\| Sbjct 150 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQMDVVNAFQS 204
Procambarus clarkii	Query 1071 IDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRS 1104 \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\| \|\|+\| Sbjct 1119 IDHAERELR#RGQILWFRGLNRIQTQIEVVNAFKS 1152

* References

[PubMed ID: 18848933] Souayah N, Sharovetskaya A, Kurnellas MP, Myerson M, Deitch JS, Elkabes S, Reductions in motor unit number estimates (MUNE) precede motor neuron loss in the plasma membrane calcium ATPase 2 (PMCA2)-heterozygous mice. Exp Neurol. 2008 Dec;214(2):341-6. Epub 2008 Sep 19.

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[PubMed ID: 18974863] ... Spiden SL, Bortolozzi M, Di Leva F, de Angelis MH, Fuchs H, Lim D, Ortolano S, Ingham NJ, Brini M, Carafoli E, Mammano F, Steel KP, The novel mouse mutation Oblivion inactivates the PMCA2 pump and causes progressive hearing loss. PLoS Genet. 2008 Oct;4(10):e1000238. Epub 2008 Oct 31.

[PubMed ID: 18280673] ... Sun XY, Chen ZY, Hayashi Y, Kanou Y, Takagishi Y, Oda S, Murata Y, Insertion of an intracisternal A particle retrotransposon element in plasma membrane calcium ATPase 2 gene attenuates its expression and produces an ataxic phenotype in joggle mutant mice. Gene. 2008 Mar 31;411(1-2):94-102. Epub 2008 Jan 26.

[PubMed ID: 17938178] ... Baggaley E, McLarnon S, Demeter I, Varga G, Bruce JI, Differential regulation of the apical plasma membrane Ca(2+) -ATPase by protein kinase A in parotid acinar cells. J Biol Chem. 2007 Dec 28;282(52):37678-93. Epub 2007 Oct 15.

[PubMed ID: 18075201] ... Sun XY, Chen ZY, Kanou Y, Takagishi Y, Hayashi Y, Ohno T, Murata Y, Oda S, Mapping of jog locus to the region between D6Mit104 and D6Mit336 on mouse chromosome 6. Exp Anim. 2007 Oct;56(5):389-92.

[PubMed ID: 7537672] ... Modolell M, Corraliza IM, Link F, Soler G, Eichmann K, Reciprocal regulation of the nitric oxide synthase/arginase balance in mouse bone marrow-derived macrophages by TH1 and TH2 cytokines. Eur J Immunol. 1995 Apr;25(4):1101-4.

[PubMed ID: 7518067] ... Ikeda M, Matsui K, Ishihara Y, Morita I, Murota S, Yuasa T, Miyatake T, Cerebellar nitric oxide synthase, cGMP and motor function in two lines of cerebellar mutant mice, Staggerer and Wriggle Mouse Sagami. Neurosci Lett. 1994 Feb 28;168(1-2):65-8.

[PubMed ID: 1974816] ... Nhamburo PT, Kimura S, McBride OW, Kozak CA, Gelboin HV, Gonzalez FJ, The human CYP2F gene subfamily: identification of a cDNA encoding a new cytochrome P450, cDNA-directed expression, and chromosome mapping. Biochemistry. 1990 Jun 12;29(23):5491-9.

[PubMed ID: 2765934] ... Ikeda M, Mikuni M, Nishikawa T, Takahashi K, A neurochemical study of a new mutant mouse presenting myoclonus-like involuntary movement: a possible model of spontaneous serotonergic hyperactivity. Brain Res. 1989 Aug 28;495(2):337-48.

[PubMed ID: 3038581] ... Matsui K, Mukoyama M, Adachi K, Ando K, [Fundamental study on ataxic mice (wriggle mouse Sagami)] Jikken Dobutsu. 1987 Apr;36(2):185-9.