CaMPDB :: Substrate

XSB2970 : adenosine triphosphatase

This entry is computationally expanded from SB0056

* Basic Information

Organism	Homo sapiens (human)
Protein Names	Plasma membrane calcium-transporting ATPase 1; PMCA1; 3.6.3.8; Plasma membrane calcium pump isoform 1; Plasma membrane calcium ATPase isoform 1
Gene Names	PMCA1
Gene Locus	Not available
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
AAA36000	N/A	N/A	AT2B1_HUMAN	N/A	N/A	N/A	hsa:490

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 962 aa

Average Mass: 106.643 kDa

Monoisotopic Mass: 106.575 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
E1-E2_ATPase 1.	15	207	4.8	4.9e-05
Hydrolase 1.	211	560	63.7	7.1e-16
Cation_ATPase_C 1.	621	803	204.3	3.3e-58

3. Sequence Information

Fasta Sequence: XSB2970.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites]

Cleavage sites (±10aa)

[Site 1] EIDHAERELR⁸⁴³-RGQILWFRGL

Arg⁸⁴³ Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Glu⁸³⁴	Ile⁸³⁵	Asp⁸³⁶	His⁸³⁷	Ala⁸³⁸	Glu⁸³⁹	Arg⁸⁴⁰	Glu⁸⁴¹	Leu⁸⁴²	Arg⁸⁴³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg⁸⁴⁴	Gly⁸⁴⁵	Gln⁸⁴⁶	Ile⁸⁴⁷	Leu⁸⁴⁸	Trp⁸⁴⁹	Phe⁸⁵⁰	Arg⁸⁵¹	Gly⁸⁵²	Leu⁸⁵³

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
AGHGTQKEEIPEEELAEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVNAFRSSLYEG

Summary

#	organism	max score	hits	top seq
1	Canis familiaris	91.30	103	PREDICTED: similar to plasma membrane calcium ATPa
2	N/A	91.30	40	I
3	Homo sapiens	91.30	27	plasma membrane calcium ATPase isoform 1
4	Bos taurus	91.30	12	plasma membrane calcium ATPase 1
5	Mus musculus	91.30	12	Atp2b1 protein
6	Gallus gallus	91.30	9	PREDICTED: similar to adenosine triphosphatase iso
7	Macaca mulatta	91.30	7	PREDICTED: similar to plasma membrane calcium ATPa
8	Monodelphis domestica	91.30	3	PREDICTED: similar to plasma membrane calcium ATPa
9	Sus scrofa	91.30	2	plasma membrane calcium ATPase 1
10	Rana catesbeiana	89.40	2	PMCA1bx
11	Tetraodon nigroviridis	84.30	4	unnamed protein product
12	Danio rerio	84.30	4	PREDICTED: hypothetical protein, partial
13	Rattus norvegicus	84.00	6	PREDICTED: similar to plasma membrane calcium ATPa
14	Pan troglodytes	82.00	11	PREDICTED: plasma membrane calcium ATPase 2 isofor
15	Oryctolagus cuniculus	80.90	1	Ca2+/Mg2+ ATPase
16	Pongo pygmaeus	75.90	1	hypothetical protein
17	synthetic construct	72.80	2	hypothetical protein
18	rats, ROS 17/2.8 osteosarcoma cells, Peptide Partial, 123 aa	71.20	1	plasma membrane Ca(2+)-ATPase PMCA1b isoform {alte
19	Procambarus clarkii	70.90	1	plasma membrane calcium ATPase PMCA3
20	Xenopus laevis	70.10	1	ATPase, Ca++ transporting, plasma membrane 3

Top-ranked sequences

organism	matching
Canis familiaris	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 661 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 720
N/A	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 814 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873
Homo sapiens	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 936 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 995
Bos taurus	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1072 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 1131
Mus musculus	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 766 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 825
Gallus gallus	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1057 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 1116
Macaca mulatta	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 955 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 1014
Monodelphis domestica	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1059 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 1118
Sus scrofa	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1072 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 1131
Rana catesbeiana	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\|\|\|\| \| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1066 AGHGTHKDEIPEEELAEDAEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 1125
Tetraodon nigroviridis	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\| \|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 288 AGQLTQKDELPEEDVNEENEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 347
Danio rerio	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\| \|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 293 AGQLTQKDEMPEEEMNEDQEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 352
Rattus norvegicus	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\|\|\| \| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1069 AGHGPGKDEMTDEELAEGEEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1128
Pan troglodytes	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\| \|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1042 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1101
Oryctolagus cuniculus	Query 816 HGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\|\|\|\| \| \|\|\|\|\|\|\|#\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1074 HGTQKREIGEEELAEDVEEIVHAERELR#RWQILWFRGLNRIQTQIRVVNAFRSSLYEG 1131
Pongo pygmaeus	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRS 868 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+ \|\|\|\|\|+\| Sbjct 150 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQMDVVNAFQS 204
synthetic construct	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYE 872 \|\|\|\|\| \| \|\|\|\|\| \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\| \|\| \|\|\|+\| Sbjct 1060 AGHGTTKEEITKDAEGLDE--IDHAEMELR#RGQILWFRGLNRIQTQIKVVKAFHSSLHE 1116
rats, ROS 17/2.8 osteosarcoma cells, Peptide Partial, 123 aa	Query 840 RELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873 \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 840 RELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 873
Procambarus clarkii	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRS 868 \|\|\| +\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|+\| Sbjct 1098 AGHEHRKDEMNAEDLNEGQEEIDHAERELR#RGQILWFRGLNRIQTQIEVVNAFKS 1152
Xenopus laevis	Query 814 AGHGTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRS 868 \|\|+ \|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| \|+\| Sbjct 1098 AGNLTQKEENQEEELNEDAEEIDHAERELR#RGQILWFRGLNRIQTQIEVVNTFKS 1152

* References

[PubMed ID: 8386431] Kumar R, Haugen JD, Penniston JT, Molecular cloning of a plasma membrane calcium pump from human osteoblasts. J Bone Miner Res. 1993 Apr;8(4):505-13.