CL0147 : calpain 11
[ CaMP Format ]
* Basic Information
| Organism | Homo sapiens (human) |
| Protein Names | Calpain 11 [Homo sapiens]; Calpain-11; 3.4.22.-; Calcium-activated neutral proteinase 11; CANP 11 |
| Gene Names | CAPN11 |
| Gene Locus | Not available |
| GO Function | Not available |
* Information From OMIM
Text: Calpains are a superfamily of related proteins, some of which have been shown to function as intracellular calcium-dependent cysteine proteases. Calpains consist of a variable large (80 kD) subunit and an invariant small (30 kD) subunit (CAPN4; OMIM:114170). By searching an EST database with the protein sequences of known vertebrate calpains, Dear et al. (1999) identified a calpain-11 (CAPN11) EST. The complete CAPN11 coding sequence encodes a deduced 702-amino acid protein with similarity to the large subunits of calpains. CAPN11 can be subdivided into the 4 domains typical of calpains. Domain II shows the characteristics of a protease domain and contains the cysteine, histidine, and asparagine residues that are part of the active site of cysteine proteases. Domain IV contains 5 putative calcium-binding EF-hand motifs. Dear et al. (1999) concluded that CAPN11 is likely to have protease activity and calcium-binding ability. Compared to all known calpains, CAPN11 shared the greatest sequence homology, 57.5%, with chicken mu/m calpain. Sequence homology between CAPN11 and known human calpains ranged from 54.3% with CAPN1 (OMIM:114220) to 18.7% with CAPN6 (OMIM:300146). Dot blot analysis of RNA from 50 different human tissues showed highest expression of CAPN11 in testis. Northern blot analysis of a variety of human tissues detected an approximately 3-kb CAPN11 transcript in testis.
* Structure Information
1. Primary Information
Length: 702 aa
Average Mass: 80.555 kDa
Monoisotopic Mass: 80.503 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| Peptidase_C2 1. | 42 | 341 | 614.1 | 1.4e-181 |
| Calpain_III 1. | 352 | 510 | 279.5 | 7.7e-81 |
| efhand 1. | 577 | 605 | 24.4 | 0.00045 |
| efhand 2. | 607 | 635 | 22.4 | 0.0019 |
3. Sequence Information
Fasta Sequence: CL0147.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* References
[PubMed ID: 12477932] Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA, Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903. Epub 2002 Dec 11.