CaMPDB :: Calpain

CL0998 : Calpain-2 catalytic subunit

* Basic Information

Organism	Rattus norvegicus (Norway rat)
Protein Names	Calpain-2 large subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; M-calpain; Millimolar-calpain; 3.4.22.53
Gene Names	Capn2
Gene Locus	Not available
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
Q07009	N/A	N/A	Q07009	N/A	N/A	N/A	rno:29154

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 700 aa

Average Mass: 79.919 kDa

Monoisotopic Mass: 79.869 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
Peptidase_C2 1.	45	344	812.0	3.8e-241
Calpain_III 1.	355	510	385.6	8.7e-113
efhand 1.	576	604	20.4	0.0074
efhand 2.	606	634	19.4	0.015
efhand 3.	671	698	3.2	9.5

3. Sequence Information

Fasta Sequence: CL0998.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1DF0 (X-ray; 260 A; A=1-700), 1MDW (X-ray; 195 A; A/B=19-346), 1QXP (X-ray; 280 A; A/B=-), 1U5I (X-ray; 286 A; A=1-700)

* References

[PubMed ID: 8218419] DeLuca CI, Davies PL, Samis JA, Elce JS, Molecular cloning and bacterial expression of cDNA for rat calpain II 80 kDa subunit. Biochim Biophys Acta. 1993 Oct 19;1216(1):81-93.

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[PubMed ID: 15489334] ... Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J, The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7.

[PubMed ID: 11342050] ... Moldoveanu T, Hosfield CM, Jia Z, Elce JS, Davies PL, Ca(2+)-induced structural changes in rat m-calpain revealed by partial proteolysis. Biochim Biophys Acta. 2001 Feb 9;1545(1-2):245-54.

[PubMed ID: 11102442] ... Hosfield CM, Moldoveanu T, Davies PL, Elce JS, Jia Z, Calpain mutants with increased Ca2+ sensitivity and implications for the role of the C(2)-like domain. J Biol Chem. 2001 Mar 9;276(10):7404-7. Epub 2000 Dec 1.

[PubMed ID: 7635186] ... Arthur JS, Gauthier S, Elce JS, Active site residues in m-calpain: identification by site-directed mutagenesis. FEBS Lett. 1995 Jul 24;368(3):397-400.

[PubMed ID: 10601010] ... Hosfield CM, Elce JS, Davies PL, Jia Z, Crystal structure of calpain reveals the structural basis for Ca(2+)-dependent protease activity and a novel mode of enzyme activation. EMBO J. 1999 Dec 15;18(24):6880-9.