XSB0243 : Gap junction alpha-8 protein
[ CaMP Format ]
This entry is computationally expanded from SB0016
* Basic Information
| Organism | Ovis aries (sheep) |
| Protein Names | Connexin-49; Cx49; Lens fiber protein MP70; MP64; MP38 |
| Gene Names | GJA8 |
| Gene Locus | Not available |
| GO Function | Not available |
| Entrez Protein |
Entrez Nucleotide |
Entrez Gene |
UniProt |
OMIM |
HGNC |
HPRD |
KEGG |
| P55917 |
N/A |
N/A |
P55917 |
N/A |
N/A |
N/A |
N/A |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 440 aa
Average Mass: 49.160 kDa
Monoisotopic Mass: 49.129 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
| Connexin 1. | 3 | 129 | 314.7 | 1.9e-91 |
| Connexin_CCC 1. | 168 | 234 | 167.1 | 5.1e-47 |
| Connexin50 1. | 267 | 333 | 185.2 | 1.9e-52 |
| --- cleavage 290 (inside Connexin50 267..333) --- |
| --- cleavage 300 (inside Connexin50 267..333) --- |
3. Sequence Information
Fasta Sequence: XSB0243.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
2 [sites]
Cleavage sites (±10aa)
[Site 1] IVSHYFPLTE290-VGMVEASPLS
Glu290 
Val
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Ile281 | Val282 | Ser283 | His284 | Tyr285 | Phe286 | Pro287 | Leu288 | Thr289 | Glu290 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Val291 | Gly292 | Met293 | Val294 | Glu295 | Ala296 | Ser297 | Pro298 | Leu299 | Ser300 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
SIAVSSIQKAKGYQLLEEEKIVSHYFPLTEVGMVEASPLSAKPFSQFEEKVGPGPLGDLS
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
N/A |
120.00 |
1 |
C |
| 2 |
Ovis aries |
120.00 |
1 |
AF177913_1 connexin 49 protein |
| 3 |
Bos taurus |
120.00 |
1 |
PREDICTED: similar to Gap junction alpha-8 protein |
| 4 |
Canis familiaris |
114.00 |
6 |
PREDICTED: similar to gap junction membrane channe |
| 5 |
Mus musculus |
112.00 |
1 |
gap junction membrane channel protein alpha 8 |
| 6 |
Mesocricetus auratus |
112.00 |
1 |
connexin50 |
| 7 |
Rattus norvegicus |
112.00 |
1 |
gap junction membrane channel protein alpha 8 |
| 8 |
Cricetulus griseus |
112.00 |
1 |
connexin50 |
| 9 |
Homo sapiens |
109.00 |
3 |
gap junction protein, alpha 8, 50kDa (connexin 50) |
| 10 |
Pan troglodytes |
109.00 |
1 |
PREDICTED: gap junction protein, alpha 8, 50kDa (c |
| 11 |
Monodelphis domestica |
104.00 |
1 |
PREDICTED: similar to Gap junction alpha-8 protein |
| 12 |
Macaca mulatta |
93.60 |
1 |
PREDICTED: gap junction protein, alpha 8, 50kDa (c |
| 13 |
Gallus gallus |
82.00 |
1 |
gap junction protein, alpha 8, 50kDa (connexin 50) |
| 14 |
Danio rerio |
64.30 |
3 |
AF288817_1 gap junction protein connexin 44.1 |
| 15 |
Tetraodon nigroviridis |
47.80 |
1 |
unnamed protein product |
Top-ranked sequences
| organism | matching |
|---|
| N/A |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
|
| Ovis aries |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
|
| Bos taurus |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
|
| Canis familiaris |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||| ||||||||||||||+| |||||||
Sbjct 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVETSPLSAKPFSQFEEKMGTGPLGDLS 320
|
| Mus musculus |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||| ||||||||||||||+| ||| |+|
Sbjct 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVETSPLSAKPFSQFEEKIGTGPLADMS 320
|
| Mesocricetus auratus |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||| ||||||||||||||+| ||| |+|
Sbjct 243 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVETSPLSAKPFSQFEEKIGTGPLADMS 302
|
| Rattus norvegicus |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||| ||||||||||||||+| ||| |+|
Sbjct 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVETSPLSAKPFSQFEEKIGTGPLADMS 320
|
| Cricetulus griseus |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||| ||||||||||||||+| ||| |+|
Sbjct 243 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVETSPLSAKPFSQFEEKIGTGPLADMS 302
|
| Homo sapiens |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||| ||| ||||+|||||+ |||||||
Sbjct 255 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVETSPLPAKPFNQFEEKISTGPLGDLS 314
|
| Pan troglodytes |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||| ||| ||||+|||||+ |||||||
Sbjct 254 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVETSPLPAKPFNQFEEKISTGPLGDLS 313
|
| Monodelphis domestica |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||+|| ||+| |||+||||+ |||||||
Sbjct 260 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGVVETRPLAATPFSRFEEKISTGPLGDLS 319
|
| Macaca mulatta |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||| +||||||||||+ || |||
Sbjct 260 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVE----TAKPFSQFEEKITTGPPEDLS 315
|
| Gallus gallus |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
+|||||| |||||+|||||| |||||||||#|| || ||| + |++||||+| ||| |||
Sbjct 256 AIAVSSIPKAKGYKLLEEEKPVSHYFPLTE#VG-VEPSPLPS-AFNEFEEKIGMGPLEDLS 313
|
| Danio rerio |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEK 310
||| |||||||||+||||+| ||+|||||#|| +|| | | + ||||
Sbjct 262 SIAASSIQKAKGYKLLEEDKSTSHFFPLTE#VGGMEAGRLPAS-YEPFEEK 310
|
| Tetraodon nigroviridis |
Query 261 SIAVSSIQKAKGYQLLEEEKI--VSHYFPLTE#VGMVEASPLSAKPFSQFEEK 310
|+|| |+|+ |||+|||||| ++| +|| |#||| + + || |||
Sbjct 268 SLAVPSMQRVKGYRLLEEEKAPPITHLYPLAE#VGM--EAGRGSPPFQGLEEK 317
|
[Site 2] VGMVEASPLS300-AKPFSQFEEK
Ser300 Ala
|
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Val291 | Gly292 | Met293 | Val294 | Glu295 | Ala296 | Ser297 | Pro298 | Leu299 | Ser300 |
|
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Ala301 | Lys302 | Pro303 | Phe304 | Ser305 | Gln306 | Phe307 | Glu308 | Glu309 | Lys310 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
KGYQLLEEEKIVSHYFPLTEVGMVEASPLSAKPFSQFEEKVGPGPLGDLSRAYQETLPSY
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
N/A |
124.00 |
1 |
C |
| 2 |
Ovis aries |
124.00 |
1 |
AF177913_1 connexin 49 protein |
| 3 |
Bos taurus |
124.00 |
1 |
PREDICTED: similar to Gap junction alpha-8 protein |
| 4 |
Canis familiaris |
118.00 |
6 |
PREDICTED: similar to gap junction membrane channe |
| 5 |
Mus musculus |
115.00 |
1 |
gap junction membrane channel protein alpha 8 |
| 6 |
Rattus norvegicus |
114.00 |
1 |
gap junction membrane channel protein alpha 8 |
| 7 |
Cricetulus griseus |
114.00 |
1 |
connexin50 |
| 8 |
Homo sapiens |
112.00 |
3 |
gap junction protein, alpha 8, 50kDa (connexin 50) |
| 9 |
Mesocricetus auratus |
112.00 |
1 |
connexin50 |
| 10 |
Pan troglodytes |
112.00 |
1 |
PREDICTED: gap junction protein, alpha 8, 50kDa (c |
| 11 |
Monodelphis domestica |
106.00 |
1 |
PREDICTED: similar to Gap junction alpha-8 protein |
| 12 |
Macaca mulatta |
96.30 |
1 |
PREDICTED: gap junction protein, alpha 8, 50kDa (c |
| 13 |
Gallus gallus |
84.00 |
1 |
gap junction protein, alpha 8, 50kDa (connexin 50) |
| 14 |
Danio rerio |
64.30 |
3 |
AF288817_1 gap junction protein connexin 44.1 |
| 15 |
Tetraodon nigroviridis |
53.50 |
1 |
unnamed protein product |
Top-ranked sequences
| organism | matching |
|---|
| N/A |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
|
| Ovis aries |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
|
| Bos taurus |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
|
| Canis familiaris |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||| ||||#||||||||||+| |||||||||||||||||
Sbjct 271 KGYQLLEEEKIVSHYFPLTEVGMVETSPLS#AKPFSQFEEKMGTGPLGDLSRAYQETLPSY 330
|
| Mus musculus |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||| ||||#||||||||||+| ||| |+||+||||||||
Sbjct 271 KGYQLLEEEKIVSHYFPLTEVGMVETSPLS#AKPFSQFEEKIGTGPLADMSRSYQETLPSY 330
|
| Rattus norvegicus |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||| ||||#||||||||||+| ||| |+|| ||||||||
Sbjct 271 KGYQLLEEEKIVSHYFPLTEVGMVETSPLS#AKPFSQFEEKIGTGPLADMSRGYQETLPSY 330
|
| Cricetulus griseus |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||| ||||#||||||||||+| ||| |+|| ||||||||
Sbjct 253 KGYQLLEEEKIVSHYFPLTEVGMVETSPLS#AKPFSQFEEKIGTGPLADMSRGYQETLPSY 312
|
| Homo sapiens |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||| ||| #||||+|||||+ |||||||| ||||||||
Sbjct 265 KGYQLLEEEKIVSHYFPLTEVGMVETSPLP#AKPFNQFEEKISTGPLGDLSRGYQETLPSY 324
|
| Mesocricetus auratus |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||| ||||#||||||||||+| ||| |+|| | ||||||
Sbjct 253 KGYQLLEEEKIVSHYFPLTEVGMVETSPLS#AKPFSQFEEKIGTGPLADMSRGYPETLPSY 312
|
| Pan troglodytes |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||| ||| #||||+|||||+ |||||||| ||||||||
Sbjct 264 KGYQLLEEEKIVSHYFPLTEVGMVETSPLP#AKPFNQFEEKISTGPLGDLSRGYQETLPSY 323
|
| Monodelphis domestica |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||+|| ||+#| |||+||||+ |||||||||| ||||||
Sbjct 270 KGYQLLEEEKIVSHYFPLTEVGVVETRPLA#ATPFSRFEEKISTGPLGDLSRAYTETLPSY 329
|
| Macaca mulatta |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||| +#||||||||||+ || |||| ||||||||
Sbjct 270 KGYQLLEEEKIVSHYFPLTEVGMVE----T#AKPFSQFEEKITTGPPEDLSRGYQETLPSY 325
|
| Gallus gallus |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
|||+|||||| ||||||||||| || ||| #+ |++||||+| ||| |||||+ | ||||
Sbjct 266 KGYKLLEEEKPVSHYFPLTEVG-VEPSPLP#S-AFNEFEEKIGMGPLEDLSRAFDERLPSY 323
|
| Danio rerio |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEK--VGPGPLGDLSRAYQETLP 328
|||+||||+| ||+||||||| +|| | #| + |||| | |+|+ | ||||
Sbjct 272 KGYKLLEEDKSTSHFFPLTEVGGMEAGRLP#AS-YEPFEEKSDEAMAPKKDMSKMYDETLP 330
Query 329 SY 330
||
Query 329 SY 330
|
| Tetraodon nigroviridis |
Query 271 KGYQLLEEEKI--VSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPG-PLGDLSRAYQETL 327
|||+|||||| ++| +|| |||| #+ || ||| |+ |+|+ | |||
Sbjct 278 KGYRLLEEEKAPPITHLYPLAEVGMEAGR--G#SPPFQGLEEKPEEVLPMEDISKVYDETL 335
Query 328 PSY 330
|||
Query 328 PSY 330
|
* References
[PubMed ID: 8654111] Yang DI, Louis CF, Molecular cloning of sheep connexin49 and its identity with MP70. Curr Eye Res. 1996 Mar;15(3):307-14.
» More (1) « Hide Below
[PubMed ID: 2830542] ... Kistler J, Christie D, Bullivant S, Homologies between gap junction proteins in lens, heart and liver. Nature. 1988 Feb 25;331(6158):721-3.
