XSB0430 : protein kinase C, beta isoform 1 [Homo sapiens]
[ CaMP Format ]
This entry is computationally expanded from SB0039
* Basic Information
| Organism | Homo sapiens (human) |
| Protein Names | Protein kinase C beta type; PKC-beta; PKC-B; 2.7.11.13; protein kinase C; beta isoform 1; beta 1 polypeptide |
| Gene Names | PRKCB; PKCB, PRKCB1; PKCB; PRKCB1; protein kinase C, beta |
| Gene Locus | 16p11.2; chromosome 16 |
| GO Function | Not available |
* Information From OMIM
Description: PRKCB1 is a member of the protein kinase C (PKC) gene family (see Coussens et al., 1986 and PRKCA; OMIM:176960).
Function: The 66-kD isoform of the growth factor adaptor SHC, p66(SHC) (OMIM:600560), translates oxidative damage into cell death by acting as a reactive oxygen species producer within mitochondria. Pinton et al. (2007) demonstrated that protein kinase C-beta, activated by oxidative conditions in the cell, induces phosphorylation of p66(SHC) and triggers mitochondrial accumulation of the protein after it is recognized by the prolyl isomerase PIN1 (OMIM:601052). Once imported, p66(SHC) causes alterations of mitochondrial calcium ion responses and 3-dimensional structure, thus causing apoptosis. Pinton et al. (2007) concluded that their data identified a signaling route that activates an apoptotic inducer shortening the life span.
* Structure Information
1. Primary Information
Length: 671 aa
Average Mass: 76.869 kDa
Monoisotopic Mass: 76.819 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| C1_1 1. | 37 | 89 | 80.1 | 8.2e-21 |
| C1_1 2. | 102 | 154 | 97.3 | 5.4e-26 |
| C2 1. | 173 | 260 | 126.7 | 7.5e-35 |
| --- cleavage 320 --- | ||||
| Pkinase 1. | 342 | 600 | 262.2 | 1.2e-75 |
| Pkinase_C 1. | 620 | 666 | 71.9 | 2.4e-18 |
3. Sequence Information
Fasta Sequence: XSB0430.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Known Structures in PDB: 2I0E (X-ray; 260 A; A/B=321-661)
* Cleavage Information
1 [sites]
Cleavage sites (±10aa)
[Site 1] SQGTKVPEEK320-TTNTVSKFDN
Lys320 
Thr
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Ser311 | Gln312 | Gly313 | Thr314 | Lys315 | Val316 | Pro317 | Glu318 | Glu319 | Lys320 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Thr321 | Thr322 | Asn323 | Thr324 | Val325 | Ser326 | Lys327 | Phe328 | Asp329 | Asn330 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| PPEGSEANEELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNRDRMKLTDFNFLMVLGK |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Homo sapiens | 124.00 | 8 | protein kinase C, beta isoform 1 |
| 2 | Macaca mulatta | 122.00 | 6 | PREDICTED: protein kinase C, beta isoform 1 |
| 3 | Oryctolagus cuniculus | 119.00 | 3 | KPCB_RABIT Protein kinase C beta type (PKC-beta) ( |
| 4 | Canis familiaris | 118.00 | 3 | PREDICTED: similar to Protein kinase C, beta type |
| 5 | N/A | 117.00 | 10 | K |
| 6 | Mus musculus | 117.00 | 4 | protein kinase C, beta 1 |
| 7 | Rattus norvegicus | 117.00 | 4 | unnamed protein product |
| 8 | Bos taurus | 114.00 | 3 | Unknown (protein for MGC:148660) |
| 9 | Monodelphis domestica | 109.00 | 3 | PREDICTED: similar to Protein kinase C beta type ( |
| 10 | Gallus gallus | 103.00 | 3 | PREDICTED: similar to protein kinase C beta-1 |
| 11 | Latimeria chalumnae | 94.40 | 2 | protein kinase C beta 1 |
| 12 | Protopterus dolloi | 91.30 | 2 | protein kinase C beta 1 |
| 13 | Danio rerio | 88.20 | 6 | protein kinase C, beta 1 |
| 14 | Tetraodon nigroviridis | 87.40 | 2 | unnamed protein product |
| 15 | Scyliorhinus canicula | 84.30 | 2 | protein kinase C |
| 16 | Petromyzon marinus | 55.80 | 1 | protein kinase C |
| 17 | Takifugu rubripes | 55.10 | 1 | protein kinase C, alpha type |
| 18 | synthetic construct | 53.50 | 1 | protein kinase C alpha |
| 19 | Xenopus tropicalis | 48.10 | 1 | hypothetical protein LOC780209 |
| 20 | Xenopus laevis | 47.40 | 1 | protein kinase C, alpha |
| 21 | Caenorhabditis briggsae | 43.10 | 1 | Hypothetical protein CBG16150 |
| 22 | Myxine glutinosa | 38.90 | 1 | protein kinase C |
| 23 | Aplysia californica | 38.10 | 1 | KPC1_APLCA Calcium-dependent protein kinase C (APL |
| 24 | Branchiostoma lanceolatum | 35.40 | 1 | protein kinase C |
| 25 | Aedes aegypti | 35.40 | 1 | protein kinase c |
| 26 | Apis mellifera | 33.90 | 1 | PREDICTED: similar to Protein C kinase 53E CG6622- |
| 27 | Tribolium castaneum | 32.70 | 1 | PREDICTED: similar to CG1954-PA |
| 28 | Phaeosphaeria nodorum SN15 | 32.30 | 1 | hypothetical protein SNOG_03257 |
| 29 | Trichomonas vaginalis G3 | 32.30 | 1 | SMC family, C-terminal domain containing protein |
Top-ranked sequences
| organism | matching |
|---|---|
| Homo sapiens | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |
| Macaca mulatta | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |||||| |||||||||||||||||||||||#|||||||||||||||||||||||||||||| Sbjct 291 PPEGSEGNEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |
| Oryctolagus cuniculus | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |||||| ||||||||||||| |||| ||||#||||+||||||||||||||||||||||||| Sbjct 291 PPEGSEGNEELRQKFERAKIGQGTKTPEEK#TTNTISKFDNNGNRDRMKLTDFNFLMVLGK 350 |
| Canis familiaris | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |||||| |||||||||||||||||| ||||#||||+|| |||||||||||||||||||||| Sbjct 283 PPEGSEGNEELRQKFERAKISQGTKAPEEK#TTNTISKLDNNGNRDRMKLTDFNFLMVLGK 342 |
| N/A | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |||||| ||||||||||||| |||| ||||#| ||+||||||||||||||||||||||||| Sbjct 291 PPEGSEGNEELRQKFERAKIGQGTKAPEEK#TANTISKFDNNGNRDRMKLTDFNFLMVLGK 350 |
| Mus musculus | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |||||| ||||||||||||| |||| ||||#| ||+||||||||||||||||||||||||| Sbjct 291 PPEGSEGNEELRQKFERAKIGQGTKAPEEK#TANTISKFDNNGNRDRMKLTDFNFLMVLGK 350 |
| Rattus norvegicus | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |||||| ||||||||||||| |||| ||||#| ||+||||||||||||||||||||||||| Sbjct 291 PPEGSEGNEELRQKFERAKIGQGTKAPEEK#TANTISKFDNNGNRDRMKLTDFNFLMVLGK 350 |
| Bos taurus | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |||||| ||||||||||||| | | ||||#||||+||||||||||||||||||||||||| Sbjct 291 PPEGSEGNEELRQKFERAKIGPGPKTPEEK#TTNTISKFDNNGNRDRMKLTDFNFLMVLGK 350 |
| Monodelphis domestica | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |||| | ||||||||||||| ||||| |||#| | +|| |||||||||||||||||||||| Sbjct 288 PPEGEEGNEELRQKFERAKIGQGTKVTEEK#TANAISKIDNNGNRDRMKLTDFNFLMVLGK 347 |
| Gallus gallus | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |||| | ||||||||||||| |+| +||#||| +|||||||+||||||+|||||||||| Sbjct 290 PPEGEEGNEELRQKFERAKIGTGSKAADEK#TTNAISKFDNNGSRDRMKLSDFNFLMVLGK 349 |
| Latimeria chalumnae | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |||| | |||||+||||||| |+| |+|# |+ ||| |||||||+||+|||||||||| Sbjct 213 PPEGEEGNEELRKKFERAKIGPGSKATEDK#VKNSSSKFGNNGNRDRIKLSDFNFLMVLGK 272 |
| Protopterus dolloi | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |||| | ||||||||||||| | + |+|# | +||||||||||+||+|||||||||| Sbjct 222 PPEGEEGNEELRQKFERAKIGPGAEAAEDK#IANA-AKFDNNGNRDRVKLSDFNFLMVLGK 280 |
| Danio rerio | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |||| | ||||||||||||| | + #++| +||||+|||||||||+|||||||||| Sbjct 290 PPEGEEGNEELRQKFERAKI--GPSKTDGS#SSNAISKFDSNGNRDRMKLSDFNFLMVLGK 347 |
| Tetraodon nigroviridis | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 |||| | ||||||||||||| | | + |#+ | |+||+|||+||||| |||||||||| Sbjct 227 PPEGEEGNEELRQKFERAKIGPG-KNTDGK#SANAASRFDSNGNQDRMKLADFNFLMVLGK 285 |
| Scyliorhinus canicula | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 | | || |||||||||||+| |+| |||# +++ || | ||||||||| |||||||||| Sbjct 212 PEEDSEGNEELRQKFERARIGPGSKAAEEK#RSSSWSKID-NGNRDRMKLADFNFLMVLGK 270 |
| Petromyzon marinus | Query 292 PEGSEANEELRQKFERAKISQGTK--VPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLG 349 |+ + + |+|+|||+|++ | +|+# |++| || | ||+| ||||||||| Sbjct 222 PDIEDGDTEMRRKFEKARLGPSVKPRASDER#RANSLSAILNNANVDRVKADDFNFLMVLG 281 Query 350 K 350 | Query 350 K 350 |
| Takifugu rubripes | Query 292 PEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 || + | |||||||+||+ || || # | +|| ||++| ||||| +||| Sbjct 289 PEVDDVNLELRQKFEKAKLGQGKKVITPS#DHRRFSL--PSGNMDRVRLNDFNFLALLGK 345 |
| synthetic construct | Query 292 PEGSE-ANEELRQKFERAKISQ-GTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLG 349 ||| | | |||||||+||+ | || # + + + + | ||+|||||||||||| Sbjct 291 PEGDEEGNMELRQKFEKAKLGPAGNKV----#ISPSEDRKQPSNNLDRVKLTDFNFLMVLG 346 Query 350 K 350 | Query 350 K 350 |
| Xenopus tropicalis | Query 291 PPEGSEANEELRQKFERAKISQ-GTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLG 349 | | + | |||||||+||+ | || # |+ + + | | ++||||+|||||| Sbjct 295 PEEEDDGNIELRQKFEKAKLGPAGNKVISP-#-TDERRPYVPSNNIDSVRLTDFSFLMVLG 352 Query 350 K 350 | Query 350 K 350 |
| Xenopus laevis | Query 292 PEGSEANEELRQKFERAKIS-QGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 || + | |||||||+||+ | || # | + + | | ++|||| ||||||| Sbjct 295 PEADDGNLELRQKFEKAKLGPAGNKV--IN#PTGERRPYIPSNNIDSIRLTDFCFLMVLGK 352 |
| Caenorhabditis briggsae | Query 292 PEGSEANEELRQKF-ERAKISQGTKV--PEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVL 348 || | |++|+| + |||+ + | | #| | | ||| +| +||||| || Sbjct 362 PEYDEDMEKVRKKMNDNFKISRDSSAGKPRES#TPRATSTSLTNTNRDVIKASDFNFLTVL 421 Query 349 GK 350 || Query 349 GK 350 |
| Myxine glutinosa | Query 292 PEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 ||| | | |||+ +|++|++ | + # + | + ||+ | || || |||| Sbjct 213 PEGEEGLE-LRQRLQRSQIARSDKNKQSP#AKDQPSDSASLSGLDRVNLEDFVFLTVLGK 270 |
| Aplysia californica | Query 295 SEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 +|+ +|++ | |+ || + || # +|| +| ++ +||||| |||| Sbjct 279 TESIQEIKSKMHRSSISSEKRYPEPD#KVQNMSK------QDIVRASDFNFLTVLGK 328 |
| Branchiostoma lanceolatum | Query 300 ELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 +|++ ++ |+ + | ++#+ | |+ | || |||||||||| Sbjct 230 KLKEHLQKQKLDEQRKQKSKR#SEEC-----NIGSLDHMKAADFNFLMVLGK 275 |
| Aedes aegypti | Query 291 PPEGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 | ||++ +|+ + + ||+ | +|# | | +| ++ ||||||||||| Sbjct 239 PEEGTDL-VQLKSQMRKTSISKKAPVLCDK#DVP-----HNMGKKDVIRATDFNFLMVLGK 292 |
| Apis mellifera | Query 312 QGTKVPEEK#TTNTVSKFDNN-GNRDRMKLTDFNFLMVLGK 350 | | | ++ #|| + +| | | ++ +|||||||||| Sbjct 324 QKTSVTKKT#TTTQDKEVPHNMGKSDLIRASDFNFLMVLGK 363 |
| Tribolium castaneum | Query 293 EGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKLTDFNFLMVLGK 350 || |+ + | + ||+ |||# | ++ ++ | ||||+ |||| Sbjct 311 EGQSENQAPEDEMENTD-ERLTKMMEEK#MANNGGDGAERKSKSKIGLEDFNFIKVLGK 367 |
| Phaeosphaeria nodorum SN15 | Query 296 EANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNGNRDRMKL 339 | | | ++| || | + | ||++ # + || |+ ||| Sbjct 47 EVNAHLTERFGRASIRESTAVPDDN#IPTSAPARINNDIREYMKL 90 |
| Trichomonas vaginalis G3 | Query 293 EGSEANEELRQKFERAKISQGTKVPEEK#TTNTVSKFDNNG-NRDRMKLTD 341 | + ||+| |+ |+|| |+ |||#| | | + | + || | Sbjct 436 ENKKLNEKLNQELEKAKSDLSQKIEEEK#TLNGKSDIEEKTLNSLKEKLED 485 |
* References
[PubMed ID: 19168795] Holler C, Pinon JD, Denk U, Heyder C, Hofbauer S, Greil R, Egle A, PKCbeta is essential for the development of chronic lymphocytic leukemia in the TCL1 transgenic mouse model: validation of PKCbeta as a therapeutic target in chronic lymphocytic leukemia. Blood. 2009 Mar 19;113(12):2791-4. Epub 2009 Jan 23.
[PubMed ID: 19091746] ... Gould CM, Kannan N, Taylor SS, Newton AC, The chaperones Hsp90 and Cdc37 mediate the maturation and stabilization of protein kinase C through a conserved PXXP motif in the C-terminal tail. J Biol Chem. 2009 Feb 20;284(8):4921-35. Epub 2008 Dec 17.
[PubMed ID: 19112601] ... Guo K, Li Y, Kang X, Sun L, Cui J, Gao D, Liu Y, Role of PKCbeta in hepatocellular carcinoma cells migration and invasion in vitro: a potential therapeutic target. Clin Exp Metastasis. 2009;26(3):189-95. Epub 2008 Dec 27.
[PubMed ID: 18981301] ... Osto E, Kouroedov A, Mocharla P, Akhmedov A, Besler C, Rohrer L, von Eckardstein A, Iliceto S, Volpe M, Luscher TF, Cosentino F, Inhibition of protein kinase Cbeta prevents foam cell formation by reducing scavenger receptor A expression in human macrophages. Circulation. 2008 Nov 18;118(21):2174-82. Epub 2008 Nov 3.
[PubMed ID: 18937679] ... Pettigrew KA, McKnight AJ, Martin RJ, Patterson CC, Kilner J, Sadlier D, Maxwell AP, Savage DA, No support for association of protein kinase C, beta 1 (PRKCB1) gene promoter polymorphisms c.-1504C>T and c.-546C>G with diabetic nephropathy in Type 1 diabetes. Diabet Med. 2008 Sep;25(9):1127-9.
[PubMed ID: 8749392] ... Keranen LM, Dutil EM, Newton AC, Protein kinase C is regulated in vivo by three functionally distinct phosphorylations. Curr Biol. 1995 Dec 1;5(12):1394-1403.
[PubMed ID: 7961692] ... Orr JW, Newton AC, Requirement for negative charge on "activation loop" of protein kinase C. J Biol Chem. 1994 Nov 4;269(44):27715-8.
[PubMed ID: 8327493] ... Zhang J, Wang L, Petrin J, Bishop WR, Bond RW, Characterization of site-specific mutants altered at protein kinase C beta 1 isozyme autophosphorylation sites. Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6130-4.
[PubMed ID: 1400396] ... Obeid LM, Blobe GC, Karolak LA, Hannun YA, Cloning and characterization of the major promoter of the human protein kinase C beta gene. Regulation by phorbol esters. J Biol Chem. 1992 Oct 15;267(29):20804-10.
[PubMed ID: 1324914] ... Goode N, Hughes K, Woodgett JR, Parker PJ, Differential regulation of glycogen synthase kinase-3 beta by protein kinase C isotypes. J Biol Chem. 1992 Aug 25;267(24):16878-82.
[PubMed ID: 1321150] ... Moss SJ, Doherty CA, Huganir RL, Identification of the cAMP-dependent protein kinase and protein kinase C phosphorylation sites within the major intracellular domains of the beta 1, gamma 2S, and gamma 2L subunits of the gamma-aminobutyric acid type A receptor. J Biol Chem. 1992 Jul 15;267(20):14470-6.
[PubMed ID: 1611098] ... Devalia V, Thomas NS, Roberts PJ, Jones HM, Linch DC, Down-regulation of human protein kinase C alpha is associated with terminal neutrophil differentiation. Blood. 1992 Jul 1;80(1):68-76.
[PubMed ID: 1556124] ... Niino YS, Ohno S, Suzuki K, Positive and negative regulation of the transcription of the human protein kinase C beta gene. J Biol Chem. 1992 Mar 25;267(9):6158-63.