XSB0604 : Chain A, Crystal Structure Analysis Of Rat M-Calpain Mutant Lys10 Thr
[ CaMP Format ]
This entry is computationally expanded from SB0001
* Basic Information
| Organism | Rattus norvegicus (Norway rat) |
| Protein Names | Calpain-2 catalytic subunit; 3.4.22.53; Calpain-2 large subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; M-calpain; Millimolar-calpain |
| Gene Names | Capn2 |
| Gene Locus | Not available |
| GO Function | Not available |
| Entrez Protein |
Entrez Nucleotide |
Entrez Gene |
UniProt |
OMIM |
HGNC |
HPRD |
KEGG |
| 1U5I_A |
N/A |
N/A |
Q07009 |
N/A |
N/A |
N/A |
rno:29154 |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 700 aa
Average Mass: 79.876 kDa
Monoisotopic Mass: 79.826 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
| --- cleavage 9 --- |
| Peptidase_C2 1. | 45 | 344 | 802.7 | 2.3e-238 |
| Calpain_III 1. | 355 | 510 | 385.6 | 8.7e-113 |
| efhand 1. | 576 | 604 | 20.4 | 0.0074 |
| efhand 2. | 606 | 634 | 19.4 | 0.015 |
| efhand 3. | 671 | 698 | 3.2 | 9.5 |
3. Sequence Information
Fasta Sequence: XSB0604.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Known Structures in PDB:
1DF0 (X-ray; 260 A; A=1-700), 1MDW (X-ray; 195 A; A/B=19-346), 1QXP (X-ray; 280 A; A/B=1-700), 1U5I (X-ray; 286 A; A=1-700), 3BOW (X-ray; 240 A; A=1-700), 3DF0 (X-ray; 295 A; A=1-700)
* Cleavage Information
1 [sites]
Cleavage sites (±10aa)
[Site 1] MAGIAMKLA9-TDREAAEGLG
Ala9 
Thr
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| - | Met1 | Ala2 | Gly3 | Ile4 | Ala5 | Met6 | Lys7 | Leu8 | Ala9 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Thr10 | Asp11 | Arg12 | Glu13 | Ala14 | Ala15 | Glu16 | Gly17 | Leu18 | Gly19 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
MAGIAMKLATDREAAEGLGSHERAIKYLNQDYETLRNEC
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
N/A |
81.60 |
12 |
A |
| 2 |
Rattus norvegicus |
79.30 |
5 |
calpain 2 |
| 3 |
Mus musculus |
77.80 |
15 |
unnamed protein product |
| 4 |
Homo sapiens |
75.10 |
17 |
calpain 2, large subunit |
| 5 |
catalytic |
75.10 |
1 |
Calpain 2, large |
| 6 |
Macaca mulatta |
73.60 |
3 |
PREDICTED: calpain 2, large subunit |
| 7 |
Bos taurus |
70.50 |
6 |
Unknown (protein for MGC:138930) |
| 8 |
Monodelphis domestica |
63.20 |
2 |
PREDICTED: similar to calpain 2 |
| 9 |
Gallus gallus |
53.90 |
5 |
PREDICTED: similar to calpain 8 (nCL-2) |
| 10 |
Xenopus tropicalis |
53.90 |
5 |
calpain 2, (m/II) large subunit |
| 11 |
Xenopus laevis |
52.80 |
9 |
hypothetical protein LOC399075 |
| 12 |
Coturnix coturnix |
50.80 |
1 |
quail calpain |
| 13 |
Canis familiaris |
49.70 |
4 |
PREDICTED: similar to calpain 8 |
| 14 |
Danio rerio |
49.30 |
23 |
hypothetical protein LOC445091 |
| 15 |
Stizostedion vitreum vitreum |
48.50 |
1 |
putative calpain-like protein |
| 16 |
Oncorhynchus mykiss |
48.10 |
3 |
calpain 2 catalytic subunit |
| 17 |
Tetraodon nigroviridis |
46.60 |
5 |
unnamed protein product |
| 18 |
Pan troglodytes |
44.30 |
5 |
PREDICTED: similar to calpain, partial |
| 19 |
Pongo pygmaeus |
42.00 |
1 |
CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1 |
| 20 |
synthetic construct |
42.00 |
1 |
Homo sapiens calpain 1, (mu/I) large subunit |
| 21 |
Sus scrofa |
40.80 |
2 |
micromolar calcium-activated neutral protease 1 is |
Top-ranked sequences
| organism | matching |
|---|
| N/A |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|||||||||#||||||||||||||||||||||||||||||
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|
| Rattus norvegicus |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|||||||||# |||||||||||||||||||||||||||||
Sbjct 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|
| Mus musculus |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|||||+|||# |||||||||||||||||||||||||||||
Sbjct 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|
| Homo sapiens |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
||||| |||# ||||||||||||||||||||||| |||||
Sbjct 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39
|
| catalytic |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
||||| |||# ||||||||||||||||||||||| |||||
Sbjct 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68
|
| Macaca mulatta |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
||||| || # ||||||||||||||||||||||| |||||
Sbjct 1 MAGIAAKLV#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39
|
| Bos taurus |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
||||| |||# ||||||||||||||+||||||| ||+||
Sbjct 1 MAGIAAKLA#KDREAAEGLGSHERAVKYLNQDYAALRDEC 39
|
| Monodelphis domestica |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|+||| |+ # ||||| |||+||+|+|||||||||| +||
Sbjct 1 MSGIAAKVV#RDREAAAGLGTHEQAVKYLNQDYETLLSEC 39
|
| Gallus gallus |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|||+| +| # +| |||||||+ +|| || |||| || |
Sbjct 1 MAGLAARLC#QERAAAEGLGSYRKAISYLGQDYEALRQRC 39
|
| Xenopus tropicalis |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|+|+| +||# +| | |||++|+|+ ||+|||| |||||
Sbjct 1 MSGVADRLA#KERALASGLGTNEKALPYLSQDYEALRNEC 39
|
| Xenopus laevis |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|+|+| +||# +| | | |++|+|+ |||||+| |||||
Sbjct 1 MSGVADRLA#KERARASGFGTNEKALPYLNQDFEALRNEC 39
|
| Coturnix coturnix |
Query 3 GIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
||| +| # || |||+| | |+|||||||| |+ |
Sbjct 6 GIAARLQ#RDRLRAEGVGEHNNAVKYLNQDYEALKQAC 42
|
| Canis familiaris |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|+ +| ++|# + || |||||++|+||| ||+| || +|
Sbjct 94 MSALAARIA#KQQAAAGGLGSHQKAVKYLGQDFEKLRQQC 132
|
| Danio rerio |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|+|||+|| # | | |+|++ +|+|+|||||| |+ ||
Sbjct 1 MSGIAIKLQ#HKRAQAAGIGTNAQAVKFLNQDYEALKREC 39
|
| Stizostedion vitreum vitreum |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|+||| | # || +|+|| +|+|||||||| || |
Sbjct 1 MSGIAATLQ#RRREKEQGIGSCSQAVKYLNQDYEALRRRC 39
|
| Oncorhynchus mykiss |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|||+| ||# | | | |++ | ||||||+|+||+||
Sbjct 1 MAGVASTLA#KKRALAAGFGTNANASKYLNQDFESLRSEC 39
|
| Tetraodon nigroviridis |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|+||| || # |+ |+| |+ +| ||||||+|+|| |
Sbjct 1 MSGIAAKLQ#HHRDRAQGFGTASQAQKYLNQDFESLRESC 39
|
| Pan troglodytes |
Query 1 MAGIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|| | ++# | | +||||++ |+||| ||++||| +|
Sbjct 1 MAAQAAGVS#RQRAATQGLGSNQNALKYLGQDFKTLRQQC 39
|
| Pongo pygmaeus |
Query 3 GIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|++ ++ # | ||| || ||||| |||| || |
Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49
|
| synthetic construct |
Query 3 GIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|++ ++ # | ||| || ||||| |||| || |
Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49
|
| Sus scrofa |
Query 3 GIAMKLA#TDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|++ ++ # | ||| || ||||| |||| || |
Sbjct 13 GVSAQVQ#KLRAKELGLGRHENAIKYLGQDYEQLRAHC 49
|
* References
[PubMed ID: 15476820] Hosfield CM, Elce JS, Jia Z, Activation of calpain by Ca2+: roles of the large subunit N-terminal and domain III-IV linker peptides. J Mol Biol. 2004 Oct 29;343(4):1049-53.
