CaMPDB :: Substrate

XSB0639 : cortactin isoform a variant [Homo sapiens]

This entry is computationally expanded from SB0022

* Basic Information

Organism	Homo sapiens (human)
Protein Names	cortactin isoform a variant
Gene Names	Not available
Gene Locus	Not available
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
BAD96333	N/A	N/A	Q53HG7	N/A	N/A	N/A	hsa:2017

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 550 aa

Average Mass: 61.558 kDa

Monoisotopic Mass: 61.521 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
HS1_rep 1.	83	119	68.2	3.1e-17
HS1_rep 2.	120	156	72.7	1.3e-18
HS1_rep 3.	157	193	74.4	4.1e-19
HS1_rep 4.	194	230	69.9	9.2e-18
HS1_rep 5.	231	267	68.5	2.5e-17
HS1_rep 6.	268	304	70.1	8.3e-18
HS1_rep 7.	305	341	29.7	1.2e-05
--- cleavage 336 (inside HS1_rep 305..341) ---
PSD5 1.	422	472	-25.5	5
SH3_1 1.	495	549	90.1	8e-24

3. Sequence Information

Fasta Sequence: XSB0639.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites]

Cleavage sites (±10aa)

[Site 1] VTQVSSAYQK³³⁶-TVPVEAVTSK

Lys³³⁶ Thr

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Val³²⁷	Thr³²⁸	Gln³²⁹	Val³³⁰	Ser³³¹	Ser³³²	Ala³³³	Tyr³³⁴	Gln³³⁵	Lys³³⁶

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Thr³³⁷	Val³³⁸	Pro³³⁹	Val³⁴⁰	Glu³⁴¹	Ala³⁴²	Val³⁴³	Thr³⁴⁴	Ser³⁴⁵	Lys³⁴⁶

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
GGKYGVQKDRMDKNASTFEDVTQVSSAYQKTVPVEAVTSKTSNIRANFENLAKEKEQEDR

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	120.00	10	SRC8_HUMAN Src substrate cortactin (Amplaxin) (Onc
2	synthetic construct	120.00	3	cortactin
3	Macaca mulatta	120.00	2	PREDICTED: similar to cortactin isoform a
4	Pongo pygmaeus	118.00	1	hypothetical protein
5	Mus musculus	113.00	5	cortactin
6	Rattus norvegicus	113.00	4	cortactin isoform B
7	Bos taurus	113.00	2	cortactin
8	N/A	113.00	2	S
9	mice, BALB/c 3T3 cells, Peptide, 546 aa	113.00	1	cortactin, p80/p85
10	Canis familiaris	112.00	5	PREDICTED: similar to cortactin isoform a isoform
11	Macaca fascicularis	99.40	2	unnamed protein product
12	Gallus gallus	98.60	2	cortactin
13	Danio rerio	87.00	4	cortactin
14	Tetraodon nigroviridis	81.30	3	unnamed protein product
15	Strongylocentrotus purpuratus	52.40	1	SRC8 protein
16	Drosophila melanogaster	48.10	2	Cortactin CG3637-PA
17	Drosophila pseudoobscura	48.10	1	GA17577-PA
18	Pan troglodytes	43.50	5	PREDICTED: cortactin isoform 3
19	Tribolium castaneum	42.70	1	PREDICTED: similar to Xaa-Pro dipeptidase (X-Pro d
20	Monodelphis domestica	39.70	1	PREDICTED: hypothetical protein
21	Suberites domuncula	38.10	1	cortactin
22	Anopheles gambiae str. PEST	33.50	1	ENSANGP00000029554
23	Trichomonas vaginalis G3	33.50	1	hypothetical protein TVAG_334050
24	Xenopus laevis	32.70	3	hypothetical protein LOC398923
25	Xenopus tropicalis	32.70	1	cortactin
26	Aedes aegypti	32.30	1	cortactin

Top-ranked sequences

organism	matching
Homo sapiens	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366
synthetic construct	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366
Macaca mulatta	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366
Pongo pygmaeus	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 270 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVASKTSNIRANFENLAKEKEQEDR 329
Mus musculus	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\| \|\| \|\|\|\|\|#\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\| Sbjct 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366
Rattus norvegicus	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\| \|\| \|\|\|\|\|#\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\| Sbjct 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329
Bos taurus	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|#\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 307 GGKYGVQKDRMDKNASTFEDVAAVSSSYQK#TVPVEAVNSKTSNIRANFENLAKEKEQEDR 366
N/A	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\| \|\| \|\|\|\|\|#\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\| Sbjct 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366
mice, BALB/c 3T3 cells, Peptide, 546 aa	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\| \|\| \|\|\|\|\|#\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\| Sbjct 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366
Canis familiaris	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|+ \|\|\|\|#\|\|\|\|\|\|\| \|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 307 GGKYGVQKDRMDKNASTFEDVAQVAPAYQK#TVPVEAVNSRTSNIRANFENLAKEKEQEDR 366
Macaca fascicularis	Query 317 MDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 317 MDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366
Gallus gallus	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|+ + +\| \|\|\|#\| \|\|\| \| +\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 316 GGKYGVQKDRMDKNAATFEDIEKPTSTYQK#TKPVERVANKTSSIRANLENLAKEKEQEDR 375
Danio rerio	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|\|+\| + \|+\|\|\|\|#\| \|\|\|\| +\| \|+\|+\| \|\|\|+\|\|+\|\|+\|++ Sbjct 306 GGKYGVQKDRMDKSAGTFEEVQKPSAAYQK#TRPVEAASSSASSIKARFENIAKQKEEEEQ 365
Tetraodon nigroviridis	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|+\|\|\|\|\|\|\|\|\| \| \|\|\|+\| + + +\|\|\|#\| \|\|\|\| \| +\|+\| \|\|\|+\|\|+\|\| \|\|\| Sbjct 315 GGKFGVQKDRMDKAAGTFEEVEKPTPSYQK#TKPVEAAGSNAGSIKARFENMAKQKEDEDR 374
Strongylocentrotus purpuratus	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|+\|\| \|+ \| \| \| \|+ \|\|\|+\|+\|#\| \| \| \|+\| \|\| +\|+ \|+\| \| Sbjct 270 GGKFGVDKNAQDATAGGFGDMQGVSSSYKK#TRPQPPAKSGAGNMRNKFEQMAQAGEEESR 329
Drosophila melanogaster	Query 307 GGKYGVQKDRMDKNASTFEDVT----QVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKE 362 \|\|\|+\|\|\|+\|\| \|\|+\| ++ \| \|# \|\| +\| \|\|+\|\| \|\|\|\|\|\| \| Sbjct 197 GGKFGVQEDRKDKSAVGWDHKEAPQKHASQVDHK#VKPV-IEGAKPSNLRAKFENLAKNSE 255 Query 363 QEDR 366 +\| \| Sbjct 256 EESR 259
Drosophila pseudoobscura	Query 307 GGKYGVQKDRMDKNASTFEDVT----QVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKE 362 \|\|\|+\|\|\|+\|\| \|\|+\| ++ \| \|# \|\| +\| \|\|+\|\| \|\|\|\|\|\| \| Sbjct 195 GGKFGVQEDRKDKSAVGWDHKEAPQKHASQVDHK#VKPV-IEGAKPSNLRAKFENLAKNSE 253 Query 363 QEDR 366 +\| \| Sbjct 254 EESR 257
Pan troglodytes	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKT 347 \|\|\|\|\|\|\|\|\|\|\|\|\|++ + \| \| \|\|# \|\| \|+ + \| Sbjct 307 GGKYGVQKDRMDKSSPSLGQTIVESPAMQK#GFPVAAIVTTT 347
Tribolium castaneum	Query 307 GGKYGVQKDRMDKNASTFED-VTQVSSAYQK#TVPVEAVTSKTSNIRANFENL 357 \|\|\|+\|+\| \|\|+\|\|+\|+ \| \| +\| + \| \|# \| + +\| ++\|\| \|\|\|+ Sbjct 736 GGKFGLQTDRVDKSAANFNDEPAKVGTNYTK#VKP-DIGDAKPKDLRAKFENM 786
Monodelphis domestica	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTS---KTSNIRANFENLAKEKEQ 363 \|\|\|\|\|\|\|\|\|\|\|\|\| \| \| \| \|#+ + \| \| \|++ \| \| + \| Sbjct 270 GGKYGVQKDRMDKRGGTAEPQAQSGRPQTK#SSLLTLATGVPCSISGIKSAFSNHQTPQAQ 329 Query 364 ED 365 \| Sbjct 330 SD 331
Suberites domuncula	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLA 358 \|\|\|\|\|\|++ \| +\| ++\|+ \| \| ++ # \| +\| +\|\|+ \|\|\|+\| Sbjct 271 GGKYGVEEGNQDSSAGGYDDMQAVKSDHRT#ERGVS--KGQTGSIRSKFENMA 320
Anopheles gambiae str. PEST	Query 307 GGKYGVQKDRMDKNASTFEDVT-----QVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEK 361 \|\|\|+\|\|\| \|\| \|\|+\| ++ \| + ++ # + + ++\| \|\|+\|\| \|\|\| \| Sbjct 195 GGKFGVQTDRKDKSAFGWDHVEKPQMHESQLDHKI#VIKPDIGSAKPSNLRAKFENFAATA 254 Query 362 EQEDR 366 \|+\| \| Sbjct 255 EEEAR 259 Query 307 GGKYGVQKDRMDKNASTFEDVTQV 330 \|\|\|+\|\|+\|\|\|\|\|\|+\| \| + +\| Sbjct 84 GGKFGVEKDRMDKSAVGHEHIEKV 107 Query 307 GGKYGVQKDRMDKNASTFEDVTQV 330 \|\|\|+\|\|\|\|\|\|+\|\|+\| ++ \| +\| Sbjct 121 GGKFGVQKDRVDKSAHGWDHVEKV 144
Trichomonas vaginalis G3	Query 317 MDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKE 362 + \| \| \|\|+\|\| + \|+\| \|# ++ + + \|++\|\| + \|\| Sbjct 42 LKKYPSAFENVTMICSSYDK#EQAIDYLMTSIKNVQANLNQIFASKE 87
Xenopus laevis	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|++\|\|\| \|\|+\|\| \|\| \| ++ \|\|\|\|+\|#\| \|+\|\|+\|\| \|+\|+ \|\|\|+\|+ \|+\|++ Sbjct 268 GGRFGVQTDRIDKCASGFGEIETPSSAYEK#TQPLEAMTSGAHNLRSRFENMARTAEEENK 327 Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#T 337 \|\|\|\|\|+\|\|\|\| \|\|+\| ++ ++ + +#\| Sbjct 194 GGKYGIQKDRQDKSAHSWSHKEELQTHQSQ#T 224 Query 307 GGKYGVQKDRMDKNASTFEDVTQV 330 \|\|+\|\|\|\| \|\|+\|\|+\| \|\| \|++ Sbjct 157 GGRYGVQTDRVDKSAVGFEYKTEL 180
Xenopus tropicalis	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|++\|\|\| \|\|+\|\| \|\| \| ++ \|\|\|\|+\|#\| \|+\|\|+\|\| \|+\|+ \|\|\|+\|+ \|+\|++ Sbjct 268 GGRFGVQTDRVDKCASGFGEIETPSSAYEK#TQPLEAMTSGAHNLRSRFENMARTAEEENK 327 Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|\|\|\|\|\|\|\| \|\|+\| ++ \| ++# \| ++ \| \| \| +\|+++\|+ Sbjct 194 GGKYGVQKDRQDKSAHSW-------SHKEE#LQPHQSQTDYTQGFGGKF---GVQKDRQDK 243 Query 307 GGKYGVQKDRMDKNASTFE 325 \|\|\|\|\|\|\|\|\|\| \|\|+\| \|+ Sbjct 120 GGKYGVQKDRTDKSAVGFD 138 Query 307 GGKYGVQKDRMDKNASTFEDVTQV 330 \|\|+\|\|\|\| \|\|+\|\|+\| \|\| \|++ Sbjct 157 GGRYGVQMDRVDKSAVGFEYKTEL 180
Aedes aegypti	Query 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 \|\|\|+\|\|\| \|\|\|\|\|+\| \|++ ++ + \| \|#\| \| + ++\| \|\|\|\|\| \|\|\| \| \|+\| \| Sbjct 231 GGKFGVQSDRMDKSALGFQEQDKIGTNYTK#TKP-DIGSAKPSNIRARFENFAMAAEEETR 289 Query 307 GGKYGVQKDRMDKNASTFEDVTQV 330 \|\|\|+\|\|\| \|\|+\|\| \|+\|+\| +\| Sbjct 120 GGKFGVQSDRVDKCAATWEHKEKV 143

* References

[PubMed ID: 8125298] Maruyama K, Sugano S, Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene. 1994 Jan 28;138(1-2):171-4.

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