CaMPDB :: Substrate

XSB2230 : glucagon preproprotein [Homo sapiens]

This entry is computationally expanded from SB0054

* Basic Information

Organism	Homo sapiens (human)
Protein Names	Glucagon; Glicentin; Glicentin-related polypeptide; GRPP; Oxyntomodulin; OXY; OXM; Glucagon-like peptide 1; GLP-1; Glucagon-like peptide 1(7-37); GLP-1(7-37); Glucagon-like peptide 1(7-36); GLP-1(7-36); Glucagon-like peptide 2; GLP-2; glucagon preproprotein; RecName: Full=Glucagon; Contains:RecName: Full=Glicentin; Contains:RecName: Full=Glicentin-related polypeptide; Short=GRPP; Contains:RecName: Full=Oxyntomodulin; Short=OXY; Short=OXM; Contains:RecName: Full=Glucagon; Contains:RecName: Full=Glucagon-like peptide 1; Short=GLP-1; 7-37; 7-36
Gene Names	GCG; glucagon
Gene Locus	2q36-q37; chromosome 2
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_002045	NM_002054	2641	GLUC_HUMAN	138030	4191	N/A	hsa:2641

* Information From OMIM

Description: Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Human, rabbit, rat, pig, and cow glucagons are identical. Glucagon is a member of a multigene family that includes secretin (OMIM:182099), vasoactive intestinal peptide (VIP; OMIM:192320), gastric inhibitory peptide (GIP; OMIM:137240), glicentin, and others.

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Function: GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus (Holz et al., 1993).

Function: GLP1 is a potent insulin secretagogue. Wang et al. (1995) presented evidence that it plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously. It is the so-called gluco-incretin. Wang et al. (1995) used an inhibitor of GLP1, called exendin(9-39), which is a fragment of a peptide found in venom of gila monsters that binds tightly to the GLP1 receptor (GLP1R; OMIM:138032) without agonistic activity. Although GLP1 and its specific receptors are present in the hypothalamus, no physiologic role for central GLP1 had been established. Turton et al. (1996) found that intracerebroventricular GLP1 powerfully inhibited feeding in fasted rats. Injection of the specific antagonist, exendin, blocked the inhibitory effect of GLP1 on food intake. Exendin alone had no influence on fast-induced feeding but more than doubled food intake in satiated rats, and augmented the feeding response to the appetite stimulus, neuropeptide Y (OMIM:162640). Induction of FOS (OMIM:164810) is a marker of neuronal activation. Following intracerebroventricular GLP1 injection, FOS appeared exclusively in the paraventricular nucleus of the hypothalamus and central nucleus of the amygdala, and this was inhibited by prior administration of exendin. Both of these regions of the brain are of primary importance in the regulation of feeding. The findings suggested that central GLP1 is a physiologic mediator of satiety.

Function: Drucker (1999) reviewed the physiologic relevance of GLP2. GLP2 is secreted in a nutrient-dependent manner from enteroendocrine cells throughout the gastrointestinal tract and is trophic to the intestinal epithelial mucosa. It acts via stimulation of crypt cell proliferation and inhibition of cell death. GLP2 also stimulates intestinal glucose transport, decreases mucosal permeability, and has shown therapeutic efficacy in experimental models of short bowel syndrome and both small and large bowel inflammation.

Function: Drucker (2001) noted that both GLP1 and GLP2 are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. GLP2 regulates gastric motility, gastric acid secretion, intestinal hexose transport, and increases the barrier function of the gut epithelium. GLP2 significantly enhances the surface area of the mucosal epithelium via stimulation of crypt cell proliferation and inhibition of apoptosis in the enterocyte and crypt compartments. The cytoprotective and reparative effects of GLP2 are evident in rodent models of experimental intestinal injury. GLP2 reduces mortality and decreases mucosal injury, cytokine expression, and bacterial septicemia in the setting of small and large bowel inflammation. GLP2 also enhances nutrient absorption and gut adaptation in rodents or humans with short bowel syndrome. The actions of GLP2 are transduced by the GLP2 receptor (OMIM:603659), a G protein-coupled receptor expressed in gut endocrine cells of the stomach, small bowel, and colon. Activation of GLP2 receptor signaling in heterologous cells promotes resistance to apoptotic injury in vitro. The authors concluded that the cytoprotective, reparative, and energy-retentive properties of GLP2 suggest that GLP2 may potentially be useful for the treatment of human disorders characterized by injury and/or dysfunction of the intestinal mucosal epithelium.

Function: GLP2 is metabolized extensively by dipeptidyl peptidase IV (DPP4; OMIM:102720) in rats. To elucidate its fate in humans, Hartmann et al. (2000) investigated GLP2 metabolism in healthy volunteers after (1) a 500-calorie mixed meal; (2) intravenous infusion of synthetic human GLP2; (3) a subcutaneous bolus injection; and (4) in vitro incubation in plasma and blood. GLP2 concentrations were determined by N-terminal RIA measuring only intact GLP2, side-viewing RIA measuring intact and degraded forms (e.g., GLP2-(3-33) arising from DPP4 degradation), and high performance liquid chromatography (HPLC). Meal ingestion elevated plasma GLP2 (intact, 16 +/- 3 to 73 +/- 10 pmol/L at 90 minutes), and HPLC revealed 2 immunoreactive components, intact GLP2 and GLP2-(3-33). The elimination t-1/2 values were 7.2 +/- 2 min (intact GLP2) and 27.4 +/- 5.4 min (GLP2-(3-33)), and MCRs were 6.8 +/- 0.6 and 1.9 +/- 0.3 mL/kg.min, respectively. Subcutaneous injection increased intact GLP2 to maximally 1,493 +/- 250 pmol/L at 45 minutes, whereas total GLP2 increased to 2,793 +/- 477 pmol/L at 90 minutes. Hartmann et al. (2000) concluded that GLP2 is extensively degraded to GLP2-(3-33) in humans, presumably by DPP4. Nevertheless, 69% remained intact 1 hour after GLP2 injection, supporting the possibility of subcutaneous use in patients with intestinal insufficiency.

Function: Pancreatic beta cells share several molecular mechanisms with pancreatic endocrine cells in terms of development, therefore they may possess a potential for insulin expression. To test this idea, Suzuki et al. (2003) sought to induce insulin-production in intestinal epithelial progenitors by using glucagon-like peptide-1. They found that GLP1-(1-37) induces insulin production in developing and, to a lesser extent, adult intestinal epithelial cells in vitro and in vivo, a process mediated by upregulation of the Notch-related gene encoding neurogenin-3 (NGN3; OMIM:604882) and its downstream targets, which are involved in pancreatic endocrine differentiation. These cells became responsive to glucose challenge in vitro and reverse insulin-dependent diabetes after implantation into diabetic mice. The findings suggested that efficient induction of insulin production in intestinal epithelial cells by GLP1-(1-37) could represent a new therapeutic approach for diabetes mellitus. The experiments were performed in the cell and organ culture.

Function: Hirasawa et al. (2005) found colocalization of GPR120 (OMIM:609044) and GLP1 in human colonic intraepithelial neuroendocrine cells. Using several techniques, including RNA interference, they showed that mouse Gpr120 mediated free fatty acid-induced secretion of Glp1 in a mouse intestinal endocrine cell line.

Function: Dyachok et al. (2006) introduced a new ratiometric evanescent-wave-microscopy approach to measure cAMP concentration beneath the plasma membrane and showed that insulin-secreting beta cells respond to glucagon and GLP1 with marked cAMP oscillations. Simultaneous measurements of intracellular calcium concentration revealed that the 2 messengers are interlinked and reinforce each other. Moreover, cAMP oscillations are capable of inducing rapid on-off calcium responses, but only sustained elevation of cAMP concentration induces nuclear translocation of the catalytic subunit of the cAMP-dependent protein kinase. Dyachok et al. (2006) concluded that their results established a new signaling mode for cAMP and indicated that temporal encoding of cAMP signals might constitute a basis for differential regulation of downstream cellular targets.

Function: Jang et al. (2007) examined human duodenal biopsy specimens and found expression of the sweet taste receptors T1R2 (OMIM:606226) and T1R3 (OMIM:605865), alpha-gustducin (GNAT3; OMIM:139395), and other taste transduction elements in the enteroendocrine L cells. Mouse intestinal cells also expressed alpha-gustducin, and ingestion of glucose by Gnat3-null mice revealed deficiencies in secretion of GLP1 and the regulation of plasma insulin (OMIM:176730) and glucose. Isolated small bowel and intestinal villi from Gnat3-null mice showed markedly defective GLP1 secretion in response to glucose. In human NCI-H716 L cells, GLP1 release was promoted by sugars and the artificial sweetener sucralose and blocked by the sweet-receptor antagonist lactisole or siRNA for alpha-gustducin. Jang et al. (2007) concluded that L cells of the gut 'taste' glucose through the same mechanism used by taste cells of the tongue.

Function: Margolskee et al. (2007) demonstrated that dietary sugar and artificial sweeteners increased sodium-dependent glucose transporter SGLT1 (SLC5A1; OMIM:182380) mRNA and protein expression and increased glucose absorptive capacity in wildtype mice but not in knockout mice lacking T1R3 or alpha-gustducin. In mouse GLUTag enteroendocrine cells, sucralose increased the release of GLP1 and GIP (OMIM:137240), gut hormones implicated in SGLT1 upregulation, and increased intracellular calcium; inhibition of the T1R2-T1R3 sweet taste receptor by gurmarin blocked the sucralose-stimulated release of GLP1 and GIP and the sucralose-dependent mobilization of calcium in GLUTag cells.

* Structure Information

1. Primary Information

Length: 180 aa

Average Mass: 20.909 kDa

Monoisotopic Mass: 20.896 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
Hormone_2 1.	53	80	66.4	1.1e-16
--- cleavage 74 (inside Hormone_2 53..80) ---
--- cleavage 69 (inside Hormone_2 53..80) ---
--- cleavage 77 (inside Hormone_2 53..80) ---
--- cleavage 67 (inside Hormone_2 53..80) ---
--- cleavage 79 (inside Hormone_2 53..80) ---
Hormone_2 2.	98	125	52.6	1.5e-12
Hormone_2 3.	146	173	45.7	1.9e-10

3. Sequence Information

Fasta Sequence: XSB2230.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1BH0 (X-ray; 300 A; A=53-81), 1D0R (NMR; -; A=98-127), 1NAU (NMR; -; A=59-81), 2G49 (X-ray; 250 A; C/D=53-81)

* Cleavage Information

5 [sites]

Cleavage sites (±10aa)

[Site 1] YLDSRRAQDF⁷⁴-VQWLMNTKRN

Phe⁷⁴ Val

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Tyr⁶⁵	Leu⁶⁶	Asp⁶⁷	Ser⁶⁸	Arg⁶⁹	Arg⁷⁰	Ala⁷¹	Gln⁷²	Asp⁷³	Phe⁷⁴

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Val⁷⁵	Gln⁷⁶	Trp⁷⁷	Leu⁷⁸	Met⁷⁹	Asn⁸⁰	Thr⁸¹	Lys⁸²	Arg⁸³	Asn⁸⁴

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
DQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHAEGTFT

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	129.00	10	glucagon, isoform CRA_b
2	N/A	79.70	44	0
3	Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 33 aa	64.70	1	AAB31092.1
4	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 29 aa	61.60	1	glucagon=proglucagon-derived 3.5 kda peptide
5	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 32 aa	60.80	1	glucagon II
6	Contains: Glucagon; Glucagon-like peptide	60.10	4	GLUC_AMICA Glucagon precursor
7	Amia calva=bowfin, pancreatic tissues, Peptide, 29 aa	60.10	1	glucagon
8	Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 29 aa	58.50	1	glucagon G-29
9	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 36 aa	57.80	1	GLUC1_ORENI Glucagon-1 (Glucagon I) gb
10	Lampetra fluviatilis=river lamprey, small intestine, Peptide, 29 aa	56.20	1	GLUC_LAMFL Glucagon gb
11	Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 29 aa	53.50	1	glucagon
12	deshis1, Desphe6, Glu9	53.50	1	A Chain A, Nmr Solution Structure Of The Glucagon
13	Monodelphis domestica	48.90	3	PREDICTED: similar to Gastric inhibitory polypepti
14	Contains: Glucagon-1 (Glucagon I); Glucagon-like peptide 1-I (GLP-1I); Glucagon-like peptide 2-I (GLP-2I)	48.90	1	GLUC1_PETMA Glucagon-1 precursor
15	Xenopus laevis	47.00	5	proglucagon II
16	Hoplobatrachus rugulosus	46.60	1	AF324209_1 proglucagon
17	Gallus gallus	46.20	4	glucagon isoform 1
18	Meleagris gallopavo	46.20	2	preproglucagon B
19	Bufo marinus	44.70	1	proglucagon
20	Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide 1 (GLP-1); Glucagon-like peptide 1(7-37) (GLP-1(7-37)); Glucagon-like peptide 1(7-36) (GLP-1(7-36)); Glucagon-like peptide 2 (GLP-2)	44.70	1	GLUC_HELSU Glucagon precursor
21	Capra hircus	43.90	1	glucagon
22	Oncorhynchus tshawytscha	43.50	1	S78474_1 proglucagon
23	Ictalurus punctatus	43.10	3	proglucagon I
24	Rattus norvegicus	42.70	5	Glucose-dependent insulinotropic peptide
25	Canis familiaris	42.70	2	PREDICTED: similar to Gastric inhibitory polypepti
26	Xenopus tropicalis	42.40	2	glucagon
27	Pan troglodytes	42.40	2	PREDICTED: fibroblast activation protein, alpha su
28	Danio rerio	42.00	6	hypothetical protein LOC494052
29	Bos taurus	42.00	5	vasoactive intestinal peptide
30	Macaca mulatta	41.60	3	PREDICTED: vasoactive intestinal peptide
31	Heloderma suspectum	41.60	2	proglucagon
32	Squalus acanthias	41.60	1	proglucagon
33	Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 32 aa	41.20	1	glucagon-like peptide, GLP
34	Contains: Glicentin-related polypeptide (GRPP); Glucagon-2 (Glucagon II); Glucagon-like peptide 2 (Glucagon-like peptide II)	41.20	1	GLUC2_LOPAM Glucagon-2 precursor (Glucagon II)
35	Sebastes caurinus	40.80	4	proglucagon I
36	Tetraodon nigroviridis	40.00	3	unnamed protein product
37	Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide	40.00	2	GLUC_CARAU Glucagon precursor
38	Contains: Glicentin-related polypeptide 1 (GRPP 1); Glucagon-1; Glucagon-like peptide 1-1 (GLP 1-1); Glucagon-like peptide 2 (GLP 2)	40.00	1	GLUC1_ONCMY Glucagon-1 precursor (Glucagon I)
39	Contains: Glicentin-related polypeptide 2 (GRPP 2); Glucagon-2; Glucagon-like peptide 1-2 (GLP 1-2); Glucagon-like peptide 2 (GLP 2)	40.00	1	GLUC2_ONCMY Glucagon-2 precursor (Glucagon II)
40	Heloderma horridum	40.00	1	EXE3_HELHO Exendin-3 precursor emb
41	Mus musculus	39.70	6	Gastric inhibitory polypeptide
42	Protopterus dolloi	38.90	1	proglucagon
43	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 33 aa	38.90	1	glucagon-like peptide-2, GLP-2=proglucagon-derived
44	Contains: Intestinal peptide PHI-42; Intestinal peptide PHI-27 (Peptide histidine isoleucinamide 27); Vasoactive intestinal peptide (VIP) (Vasoactive intestinal polypeptide)	38.50	1	VIP_MOUSE VIP peptides precursor
45	Contains: Glucagon-2 (Glucagon II) (Glu II); Glucagon-like peptide 2-II (GLP-2II)	38.10	1	GLUC2_PETMA Glucagon-2 precursor
46	Ambloplites rupestris	37.70	1	proglucagon
47	Arvicanthis ansorgei	37.70	1	vasoactive intestinal polypeptide
48	synthetic construct	37.00	1	Homo sapiens glucagon
49	Phodopus sungorus	36.60	1	glucagon precursor
50	Contains: Glicentin-related polypeptide (GRPP); Glucagon-1 (Glucagon I); Glucagon-like peptide 1 (Glucagon-like peptide I)	36.60	1	GLUC1_LOPAM Glucagon-1 precursor (Glucagon I)
51	Sus scrofa	36.20	1	glucagon
52	Neoceratodus forsteri	36.20	1	proglucagon
53	Canis lupus familiaris	35.80	1	glucagon
54	Contains: Glucagon; Glucagon-36 (Oxyntomodulin); Glucagon-like peptide	35.80	1	GLUC_LEPSP Glucagon precursor
55	Hydrolagus colliei	35.40	1	proglucagon
56	Heloderma suspectum, venom, Peptide, 39 aa	35.40	1	A Chain A, Solution Structure Of Exendin-4 In 30-V
57	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 33 aa	34.70	1	GLUC2_ORENI Glucagon-2 (Glucagon II) gb
58	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 34 aa	34.70	1	glucagon-like peptide-1, GLP-1=proglucagon-derived
59	Agkistrodon piscivorus	33.50	1	proglucagon

Top-ranked sequences

organism	matching
Homo sapiens	Query 45 DQMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 45 DQMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104
N/A	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIA 89 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 1 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNKNNIA 37
Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 33 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRN 84 \|\|+\|\|\|\|\|\|\|\|\|\|+\|+\|\|\|+\|\|#\|\|\|\|\|+\|\|\|\| Sbjct 1 HSEGTFTSDYSKYMDNRRAKDF#VQWLMSTKRN 32
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNT 81 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|#+\|\|\|\|+\| Sbjct 1 HSQGTFTSDYSKYLDNRRAQDF#IQWLMST 29
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 32 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRN 84 \|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|#\|\|\|\| \|+\|\|\| Sbjct 1 HSEGTFSNDYSKYLETRRAQDF#VQWLKNSKRN 32
Contains: Glucagon; Glucagon-like peptide	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNT 81 \|\|\|\|\|\|\|+\|\|\|\|\|+\|+\|\|\|\|\|\|#\|\|\|\|\|+\| Sbjct 1 HSQGTFTNDYSKYMDTRRAQDF#VQWLMST 29
Amia calva=bowfin, pancreatic tissues, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNT 81 \|\|\|\|\|\|\|+\|\|\|\|\|+\|+\|\|\|\|\|\|#\|\|\|\|\|+\| Sbjct 1 HSQGTFTNDYSKYMDTRRAQDF#VQWLMST 29
Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNT 81 \|\|+\|\|\|\|\|\|\|\|\|\|+\|+\|\|\|+\|\|#\|\|\|\|\|+\| Sbjct 1 HSEGTFTSDYSKYMDNRRAKDF#VQWLMST 29
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 36 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRN 84 \|\|+\|\|\|++\|\|\|\|\|\|+ \|+\|\|\|\|#\|+\|\|\|\| \|\|+ Sbjct 1 HSEGTFSNDYSKYLEDRKAQDF#VRWLMNNKRS 32
Lampetra fluviatilis=river lamprey, small intestine, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNT 81 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|++\|+\|\|#\| \|\|\|\|\| Sbjct 1 HSQGSFTSDYSKYLDSKQAKDF#VIWLMNT 29
Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMN 80 \|\|+\|\|\|\|\|\|\|\|\|\|\|++++\|+\|\|#\|+\|\|\|\| Sbjct 1 HSEGTFTSDYSKYLENKQAKDF#VRWLMN 28
deshis1, Desphe6, Glu9	Query 54 SQGTFTSDYSKYLDSRRAQDF#VQWLMNT 81 \|\|\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| Sbjct 1 SQGT-TSEYSKYLDSRRAQDF#VQWLMNT 27
Monodelphis domestica	Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGT 102 +\|+++\|\|\| \|\|\|\| +\| \|\|\|#\| \|\|++ \| +\| + \|\|+ \|\| \|+\|\| Sbjct 51 QRYAEGTFISDYSITMDKIMQQDF#VNWLLSQKGKKN--SWRHNITERKADGT 100
Contains: Glucagon-1 (Glucagon I); Glucagon-like peptide 1-I (GLP-1I); Glucagon-like peptide 2-I (GLP-2I)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|++++\|+\|\|#\|+\|\|\|\| \|\| + + + \|\|\|+\|\|\|\| Sbjct 41 KRHSEGTFTSDYSKYLENKQAKDF#VRWLMNAKRGGSELQR------RHADGTFT 88 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNR---NNIAKRHDEFERHAE 100 +\|\|+ \|\|\|\|+\| + \|\|\|++ \|+\|\|#\| \|\| + ++\| + + \| +\|\|\|\| Sbjct 80 RRHADGTFTNDMTSYLDAKAARDF#VSWLARSDKSRRDGGDHLAENSEDKRHAE 132
Xenopus laevis	Query 45 DQMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 +\|+ \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|+ +++\|+ ++\|\|\|\|\|\|\|\|\| Sbjct 45 EQLKEVKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRS-GGLSRRNADYERHAEGTFT 103 Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \| +\|\|++\|\|+\|+\| ++\|\|+ + + \|#++\|\|+ \| + + \|\|\|\|\|\|\|\| Sbjct 138 EIERHAEGTYTNDVTEYLEEKATKAF#IEWLIKGK-------PKKIRYSRHAEGTFT 186 Query 52 RHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRH 92 \|\|++\|\|\|\|+\| + \|\|+ + \|++\|#\| \|\|+\| + \| ++ + \| Sbjct 179 RHAEGTFTNDMTNYLEEKAAKEF#VGWLINGRPKRKDLLEEH 219
Hoplobatrachus rugulosus	Query 45 DQMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \| \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|+\|\|+ \|+\|\|+ +\|\|\|\|\|\|\|\|+\| Sbjct 45 DHHNEVKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNSKRS-GGISKRNVQFERHAEGTYT 103 Query 52 RHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFE-RHAEGTFT 104 \|\|++\|\|\|\|\|\| + \|\|+ + \|++\|#\| \|\|+ + \|\| +\| + \|\|\|+\|+\|\| Sbjct 134 RHAEGTFTSDMTSYLEEKAAKEF#VDWLIKGRPKRNFPDISAEEMDRRHADGSFT 187 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 +\|\|++\|\|+\|+\| +++\|+ + \|++\|#+ \|\|+ \| + ++ \|\|\|\|\|\|\|\| Sbjct 95 ERHAEGTYTNDVTQFLEEKAAKEF#IDWLIKGKPKKQRLS-------RHAEGTFT 141 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNT 81 \|+\|\|+ \|+\|\|\|\|++\| \|\| + \|\|+\|#+ \|++\|\| Sbjct 178 DRRHADGSFTSDFNKALDIKAAQEF#LDWIINT 209
Gallus gallus	Query 46 QMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRN------------------RNN 87 \|+\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\| \| Sbjct 48 QLNEVKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMSTKRNGQQGQEDKENDKFPDQLSSNA 107 Query 88 IAKRHDEFERHAEGTFT 104 \|+\|\|\| \|\|\|\|\|\|\|\|\|+\| Sbjct 108 ISKRHSEFERHAEGTYT 124 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAK---RHDEFERHAEGTFT 104 +\| +\|\|++\|\|+\|\|\| + \|\|+ + \|++\|#+ \|\|+\| + \|+ \| + \|\|\|+\|\|\|\| Sbjct 113 SEFERHAEGTYTSDITSYLEGQAAKEF#IAWLVNGRGRRDFPEKALMAEEMGRRHADGTFT 172 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\| +\| \|\| \|++\|#++\|\|+\|\|\| + ++ Sbjct 164 RRHADGTFTSDINKILDDMAAKEF#LKWLINTKVTQRDL 201
Meleagris gallopavo	Query 46 QMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRN------------------RNN 87 \|+\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\| \| Sbjct 48 QLNEVKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMSTKRNGQQGQEDKENDEFPDQLSSNA 107 Query 88 IAKRHDEFERHAEGTFT 104 \|+\|\|\| \|\|\|\|\|\|\|\|\|+\| Sbjct 108 ISKRHSEFERHAEGTYT 124 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAK---RHDEFERHAEGTFT 104 +\| +\|\|++\|\|+\|\|\| + \|\|+ + \|++\|#+ \|\|+\| + \|+ \| + \|\|\|+\|\|\|\| Sbjct 113 SEFERHAEGTYTSDITSYLEGQAAKEF#IAWLVNGRGRRDFPEKALMAEEMGRRHADGTFT 172 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\| +\| \|\| \|++\|#++\|\|+\|\|\| + ++ Sbjct 164 RRHADGTFTSDINKILDDMAAKEF#LKWLINTKVTQRDL 201
Bufo marinus	Query 52 RHSQGTFTSDYSKYLDSRRAQDF#VQWLM--NTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|++\|\|\|\|\|\| + +\|+ + \|++\|#\| \|\|+ \|\|\| ++++ \| \|\|\|+\|+\|\| Sbjct 62 RHAEGTFTSDMTSFLEEKAAKEF#VDWLIKGRPKRNFSDVSAADDMDRRHADGSFT 116 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 +\|\|++\|\|+\|+\| +++\|+ + \|++\|#+ \|\|+ \| \|+ \|\|\|\|\|\|\|\| Sbjct 23 ERHAEGTYTNDVTQFLEEKAAKEF#IDWLL------KGIPKK-QRLSRHAEGTFT 69 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNT 81 \|+\|\|+ \|+\|\|\|\|++\| \|\| + \|\|+\|#+ \|++\|\| Sbjct 107 DRRHADGSFTSDFNKALDIKAAQEF#LDWIINT 138 Query 73 DF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|# \|\|\|\|\|+\|\|+ +++\|+ +\|\|\|\|\|\|\|\|+\| Sbjct 1 DF#AQWLMNSKRS-GGMSRRNVQFERHAEGTYT 31
Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide 1 (GLP-1); Glucagon-like peptide 1(7-37) (GLP-1(7-37)); Glucagon-like peptide 1(7-36) (GLP-1(7-36)); Glucagon-like peptide 2 (GLP-2)	Query 46 QMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKR------------------NRNN 87 \|+\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\| + \| Sbjct 46 QLNEVKRHSQGTFTSDYSKYLDTRRAQDF#VQWLMNTKRSGQQGVEEREKENLLDQLSSNG 105 Query 88 IAKRHDEFERHAEGTFT 104 +\|+ \| \|+\|\|\|\|+\| +\| Sbjct 106 LARHHAEYERHADGRYT 122 Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAK---RHDEFERHAEGTFT 104 \| +\|\|+ \| +\|\|\| \| \|\|+ + \|++\|#+ \|\|+\| + \|+ + + \| \|\|\|+\|\|\|\| Sbjct 112 EYERHADGRYTSDISSYLEGQAAKEF#IAWLVNGRGRRDFLEEAGTADDIGRRHADGTFT 170 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\|\|++ \|\| \|+\|#++\|\|+\| \| + ++ Sbjct 162 RRHADGTFTSDYNQLLDDIATQEF#LKWLINQKVTQRDL 199
Capra hircus	Query 86 NNIAKRHDEFERHAEGTFT 104 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 86 NNIAKRHDEFERHAEGTFT 104 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNR 85 +\| +\|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|#+ \|\|+ + \| Sbjct 8 DEFERHAEGTFTSDVSSYLEGQAAKEF#IAWLVKGRGRR 45
Oncorhynchus tshawytscha	Query 54 SQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \| \|\|\|++\|\|\|\|\| + \| \|\|\|\|#\|\|\|\|\|\|+\|\|+ +\|\|\|+\|\|+\| Sbjct 2 SXGTFSNDYSKYQEERMAQDF#VQWLMNSKRS-------GAPSKRHADGTYT 45 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNR 85 \|\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|#\| \|\| + + \| Sbjct 37 KRHADGTYTSDVSTYLQDQAAKDF#VSWLKSGRARR 71
Ictalurus punctatus	Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 +\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|#\|\|\|\|\|\|+\|\|+ \|\|\|+\|\|+\| Sbjct 49 RRHSEGTFSNDYSKYLETRRAQDF#VQWLMNSKRS-------DSPARRHADGTYT 95 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEF--ERHAEGTFT 104 +\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|#+ \|\| + + + + \| + \|\| +\|+\|\| Sbjct 87 RRHADGTYTSDVSSYLQDQAAKDF#ITWLKSGQPKPDAVETRSVDTLRRRHVDGSFT 142 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRH 92 +\|\| \|+\|\|\|\| +\| \|\|\| \|+++#+\|\|+\|\|+ + + \|\|\| Sbjct 134 RRHVDGSFTSDVNKVLDSLAAKEY#LQWVMNSPSSAS--GKRH 173
Rattus norvegicus	Query 52 RHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHA 99 \|+++\|\|\| \|\|\|\| +\| \| \|\|\|#\| \|\|+ \| +\|+ +\|+ +\| \| Sbjct 43 RYAEGTFISDYSIAMDKIRQQDF#VNWLLAQKGKKND--WKHNLTQREA 88
Canis familiaris	Query 52 RHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHA 99 \|+++\|\|\| \|\|\|\| +\| \| \|\|\|#\| \|\|+ \| +\|+ +\|+ +\| \| Sbjct 43 RYAEGTFISDYSIAMDKIRQQDF#VNWLLAQKGKKND--WKHNITQREA 88
Xenopus tropicalis	Query 46 QMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|+ \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#+\|\|\|\|\|\|\|\|+ +++\|+ ++\|\|\|\|\|\|\|\|\| Sbjct 46 QLKEVKRHSQGTFTSDYSKYLDSRRAQDF#IQWLMNTKRS-GGLSRRNADYERHAEGTFT 103 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 +\|\|++\|\|\|\|\|\| +++\|\| + \|++\|#+ \|\|+\| ++ \|++\|+ + \|\|\|\|\|\|\|+\| Sbjct 95 ERHAEGTFTSDVTQHLDEKAAKEF#IDWLINGGPSKEIISRRNADVERHAEGTYT 148 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 +\|\|++\|\|+\|+\| ++\|\|+ + \|++\|#++\|\|+\| \| + + \|\|\|\|\|\|\|\| Sbjct 140 ERHAEGTYTNDVTEYLEEKAAKEF#IEWLINGK-------PKKIRYSRHAEGTFT 186 Query 52 RHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFE---RHAEGTFT 104 \|\|++\|\|\|\|+\| + \|\|+ + \|++\|#\| \|\|+ \| + \| ++ \| \| \|\|\|+\|+\|\| Sbjct 179 RHAEGTFTNDMTNYLEEKAAKEF#VGWLIK-GRPKRNFSEAHSVEEMDRRHADGSFT 233 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTK 82 \|+\|\|+ \|+\|\|+\| +\| \|\| \|\|+\|#+ \|++\|\|+ Sbjct 224 DRRHADGSFTNDINKVLDIIAAQEF#LDWVINTQ 256
Pan troglodytes	Query 45 DQMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\| +++\| \|\|\|\|\|\|\|\| Sbjct 967 DQMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRN-SSVA------YRHAEGTFT 1019 Query 52 RHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNR 85 \|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|#+ \|\|+ + \| Sbjct 1012 RHAEGTFTSDVSSYLEGQAAKEF#IAWLVKGRGRR 1045
Danio rerio	Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|#\|\|\|\|\|\| \|\|\| ++ +\|\|\|\|\|\|+\| Sbjct 48 KRHSEGTFSNDYSKYLETRRAQDF#VQWLMNAKRNGGSV-------KRHAEGTYT 94 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWL 78 \|\|\|++\|\|+\|\|\| \| \|\| + \|\| \|#\| \|\| Sbjct 86 KRHAEGTYTSDVSSYLQDQAAQSF#VAWL 113
Bos taurus	Query 47 MNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 ++ + \|\|+ \| \|\|\|\|\|\|+ \| \|+ +#++ \|+ \|\| \|+\|++ +\|\|++ \|\| Sbjct 75 VSRNVRHADGVFTSDYSRLLGQLSAKKY#LESLIG-KRVSNSISEDQGPIKRHSDAVFT 131
Macaca mulatta	Query 47 MNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 ++ + \|\|+ \| \|\|\|\|+\|\| \| \|+ +#++ \|\| \|\| +\|\|++ +\|\|++ \|\| Sbjct 75 VSRNARHADGVFTSDFSKLLGQLSAKKY#LESLMG-KRVSSNISEDPVPVKRHSDAVFT 131
Heloderma suspectum	Query 46 QMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKR------------------NRNN 87 \|+\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\| + \| Sbjct 46 QLNEVKRHSQGTFTSDYSKYLDTRRAQDF#VQWLMNTKRSGQQGVEEREKENLLDQLSSNG 105 Query 88 IAKRHDEFERHAEGTFT 104 +\|+ \| \|+\|\|\|\|+\| +\| Sbjct 106 LARHHAEYERHADGRYT 122 Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNR 85 \| +\|\|+ \| +\|\|\| \| \|\|+ + \|++\|#+ \|\|+\| + \| Sbjct 112 EYERHADGRYTSDISSYLEGQAAKEF#IAWLVNGRGRR 148
Squalus acanthias	Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWL--MNTKRNRNNIAKRHDEFERHAEGTF 103 ++ \|\|\|++\|\|+\|\|\| \| +\|+ \|#\| \| \| ++\| +\|+\|\|\| \| \|\| \|\|++ Sbjct 20 DKTKRHAEGTYTSDVDSLSDYFKAKRF#VDSLTSYNKRQNGRSISKRHVESTRHTEGSY 77 Query 67 DSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|+\|\|\|+\|\|#\|\|\|\|\|+\|\|\|\| \|+ +\|\|\|\|\|\|+\| Sbjct 1 DNRRAKDF#VQWLMSTKRN-------GDKTKRHAEGTYT 31 Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRN---------NIAKRHDEFERHA 99 \| \|\|++\|++ \| \| \|\|+++ \|+\|\|#+ \|\|+ + \| \| + \|\|\| Sbjct 68 ESTRHTEGSYR-DISSYLEAKAAKDF#INWLIKGRGRREFPEESKEIVNEVIPEEMDRRHA 126 Query 100 EGTFT 104 +\|\|\|\| Sbjct 127 DGTFT 131 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFE 96 \|+\|\|+ \|\|\|\|\|+ + \|\|+ \|++\|#+ \|++\|+\| \| \| \| \| Sbjct 122 DRRHADGTFTSEINIVLDTIAAKEF#LNWILNSK----TIQSRDSEIE 164
Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 32 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRN 84 \|+ \|\|\|\|+\| + \|\|\|++ \|+\|\|#\| \|\| + ++ Sbjct 1 HADGTFTNDMTSYLDAKAARDF#VSWLARSDKS 32
Contains: Glicentin-related polypeptide (GRPP); Glucagon-2 (Glucagon II); Glucagon-like peptide 2 (Glucagon-like peptide II)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|#\|\|\|\| \|+\|\|\| \| \|\|\|+\|\|+\| Sbjct 50 KRHSEGTFSNDYSKYLETRRAQDF#VQWLKNSKRN--------GLFRRHADGTYT 95 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWL 78 +\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|#\| \|\| Sbjct 87 RRHADGTYTSDVSSYLQDQAAKDF#VSWL 114
Sebastes caurinus	Query 45 DQMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|+++ \|\|\|\|+\|\|\|++\|\|\|+\|\|+ \|+\|\|\|\|#\|+\|\|\|\| \|\|+ \|\| +\|\|\|+\|\|\|\| Sbjct 42 DELSNMKRHSEGTFSNDYSRYLEERKAQDF#VRWLMNNKRS------GADE-KRHADGTFT 94 Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDE--FERHAEGTFT 104 ++\|\|\|+ \|\|\|\|\|\| \| \|\| + +\|\|#\| \| + + \| + + \| \|\| +\|+\|\| Sbjct 84 DEKRHADGTFTSDVSSYLKDQAIKDF#VNRLKSGQVRRESETEWRGEAFSRRHVDGSFT 141 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDE 94 +\|\| \|+\|\|\|\| +\| \|\|\| \|+++#+ \|+\| +\| + + ++ \|+ Sbjct 133 RRHVDGSFTSDVNKVLDSMAAKEY#LLWVMTSKPSGESKKRQEDQ 176
Tetraodon nigroviridis	Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|#\|\|\|\| \|+\|\|\| + \| \|\|\|+\|\|+\| Sbjct 787 KRHSEGTFSNDYSKYLETRRAQDF#VQWLKNSKRNGS-------LFRRHADGTYT 833 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNR 85 +\|\|+ \|\|+\|\|\| \| \|\| + \|++\|#\| \|\| + \| Sbjct 825 RRHADGTYTSDVSAYLQDQAAKEF#VSWLKTGRGRR 859
Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide	Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|#\|+\|\|\|\|+\| \| + +\|\|\|\|\|\|+\| Sbjct 48 KRHSEGTFSNDYSKYLETRRAQDF#VEWLMNSKSNGGSA-------KRHAEGTYT 94 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWL 78 \|\|\|++\|\|+\|\|\| \| +\| + \|\|+\|#\| \|\| Sbjct 86 KRHAEGTYTSDISSFLRDQAAQNF#VAWL 113
Contains: Glicentin-related polypeptide 1 (GRPP 1); Glucagon-1; Glucagon-like peptide 1-1 (GLP 1-1); Glucagon-like peptide 2 (GLP 2)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|\|+\|\|\|++\|\|\|\|\| + \| \|\|\|\|#\|\|\|\|\|\|+\|\|+ +\|\|\|+\|\|+\| Sbjct 50 KRHSEGTFSNDYSKYQEERMAQDF#VQWLMNSKRS-------GAPSKRHADGTYT 96 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNI--AKRHDEFERHAEGTFT 104 \|\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|#\| \|\| + + \| + ++ \|\| +\|+\|\| Sbjct 88 KRHADGTYTSDVSTYLQDQAAKDF#VSWLKSGRARRESAEESRNGPMSRRHVDGSFT 143 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHD 93 +\|\| \|+\|\|\|\| +\| \|\|\| \|+++#+ \|+\| +\| + + ++ \| Sbjct 135 RRHVDGSFTSDVNKVLDSLAAKEY#LLWVMTSKTSGKSNKRQED 177
Contains: Glicentin-related polypeptide 2 (GRPP 2); Glucagon-2; Glucagon-like peptide 1-2 (GLP 1-2); Glucagon-like peptide 2 (GLP 2)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\| \|+\|\|\|++ \|\|\|\| + \| \|+\|\|#+ \|\|\|\|+\|\|+ +\|\|\|+\|\|+\| Sbjct 50 KRQSEGTFSNYYSKYQEERMARDF#LHWLMNSKRS-------GAPSKRHADGTYT 96 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHD--EFERHAEGTFT 104 \|\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|#\| \|\| + \| + + + \|\| +\|+\|\| Sbjct 88 KRHADGTYTSDVSTYLQDQAAKDF#VSWLKSGPARRESAEESMNGPMSRRHVDGSFT 143 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHD 93 +\|\| \|+\|\|\|\| +\| \|\|\| \|+++#+ \|+\| +\| + + ++ \| Sbjct 135 RRHVDGSFTSDVNKVLDSLAAKEY#LLWVMTSKTSGKSNKRQED 177
Heloderma horridum	Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMN 80 \|\|\|\| \|\|\|\|\|\| \|\| ++ + \|#++\|\| \| Sbjct 46 KRHSDGTFTSDLSKQMEEEAVRLF#IEWLKN 75
Mus musculus	Query 52 RHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHA 99 \|+++\|\|\| \|\|\|\| +\| \| \|\|\|#\| \|\|+ + +++ +\|+ +\| \| Sbjct 43 RYAEGTFISDYSIAMDKIRQQDF#VNWLLAQRGKKSD--WKHNITQREA 88
Protopterus dolloi	Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \| \|\|\|\|\|\|\|\| \|\|\|+\| \|+++ \| \| #\|\| \|\|+\|\|\| ++\|\|\|+\| \|\|\|\|\|\|\|+\| Sbjct 28 EAKRHSQGTFMSDYAKLLEAKHALDL#VQRLMSTKRT-GAVSKRHNEIERHAEGTYT 82 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFE-RHAEGTFT 104 \|\| +\|\|++\|\|+\|\|\| \| \|\|+ + +\|#++\|\|\| + \|+ +\| + \|\|\|+\|\| \| Sbjct 71 NEIERHAEGTYTSDISSYLEGQAVNEF#IRWLMKGRGRRDFSETDIEEMDRRHADGTVT 128 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTK 82 \|+\|\|+ \|\| \|\|\| + \|+\| ++\|#+ \|\|+\| \| Sbjct 119 DRRHADGTVTSDINSVLESIATREF#LNWLINAK 151
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 33 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNTK 82 \|+ \|+\|\|\|\| +\| \|\|+ \|++\|#+ \|\|++\|\| Sbjct 1 HADGSFTSDINKVLDTIAAKEF#LNWLISTK 30
Contains: Intestinal peptide PHI-42; Intestinal peptide PHI-27 (Peptide histidine isoleucinamide 27); Vasoactive intestinal peptide (VIP) (Vasoactive intestinal polypeptide)	Query 47 MNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 ++ + \|\|+ \| \|\|\|\|\|\|+ \| \|+ +#++ \|+ \|\| ++\|++ +\|\|++ \|\| Sbjct 75 VSRNARHADGVFTSDYSRLLGQISAKKY#LESLIG-KRISSSISEDPVPIKRHSDAVFT 131
Contains: Glucagon-2 (Glucagon II) (Glu II); Glucagon-like peptide 2-II (GLP-2II)	Query 52 RHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|\|\|+\|\|\|\|\|\|\|+\|\| ++\|+\|\|#\| \|\|+\|\|\|\| + + +\|\|++\|+\|\| Sbjct 43 RHSQGSFTSDYSKHLDVKQAKDF#VTWLLNTKRRGVDAQAGANLEKRHSDGSFT 95 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKR 83 \| +\|\|\|\| \|+\|\|+\| + \|\| \|++\|#++\|\| \| Sbjct 84 NLEKRHSDGSFTNDMNVMLDRMSAKNF#LEWLKQQGR 119
Ambloplites rupestris	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|\|\|\|\|+\|\|+ \|\|+ \|+\|\|\|\|#++\|\|\|\| \| \|+ \|++ \|\|\|+\|\|\|\| Sbjct 1 HSQGTFTNDYTNYLEDRQAQDF#IRWLMNNK--RSGAAEK-----RHADGTFT 45 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDE--FERHAEGTFT 104 +\|\|\|+ \|\|\|\| \| \| + +\|\|#\| \| + + \| \| \| \|\| \|\|+\|\| Sbjct 36 EKRHADGTFTDDASSDFYDQAIKDF#VAKLKSGQDRREPETDRRREAFSRRHVEGSFT 92
Arvicanthis ansorgei	Query 47 MNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 ++ + \|\|+ \| \|\|\|\|\|\|+ \| \|+ +#++ \|+ \|\| ++\| + +\|\|++ \|\| Sbjct 58 VSRNARHADGVFTSDYSRLLGQISAKKY#LESLIG-KRISSSILEDPVPIKRHSDAVFT 114
synthetic construct	Query 45 DQMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 45 DQMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRN---NIAKRHDEFERHAEGTFT 104 +\| +\|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|#+ \|\|+ + \|+ +\| + \|\|\|+\|+\|+ Sbjct 93 DEFERHAEGTFTSDVSSYLEGQAAKEF#IAWLVKGRGRRDFPEEVAIVEELGRRHADGSFS 152 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTK 82 +\|\|+ \|+\|+ + + \|\|+ \|+\|\|#+ \|\|+ \|\| Sbjct 144 RRHADGSFSDEMNTILDNLAARDF#INWLIQTK 175
Phodopus sungorus	Query 56 GTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRN---NIAKRHDEFERHAEGTFT 104 \|\|\|\|\|\| \| \|\|+ + \|++\|#+ \|\|+ + \|+ + + \|\|\|+\|+\|+ Sbjct 1 GTFTSDVSSYLEGQAAKEF#IAWLVKGRGRRDFPEEVTIVEELGRRHADGSFS 52 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTK 82 +\|\|+ \|+\|+ + + \|\|\| +\|\|#+ \|\|+ \|\| Sbjct 44 RRHADGSFSDEMNTILDSLATRDF#INWLIQTK 75
Contains: Glicentin-related polypeptide (GRPP); Glucagon-1 (Glucagon I); Glucagon-like peptide 1 (Glucagon-like peptide I)	Query 46 QMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 +++ \|\|\|\|+\|\|\|++\|\|\|\|\|\|+ \|+\|\|+\|#\|+\|\|\|\| \| \|+ +\|++ \|\|\|+\|\|\|\| Sbjct 46 ELSNMKRHSEGTFSNDYSKYLEDRKAQEF#VRWLMNNK--RSGVAEK-----RHADGTFT 97 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDF#VQWL 78 +\|\|\|+ \|\|\|\|\|\| \| \|\| + +\|\|#\| \| Sbjct 88 EKRHADGTFTSDVSSYLKDQAIKDF#VDRL 116
Sus scrofa	Query 45 DQMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 45 DQMTEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNKNNIAKRHDEFERHAEGTFT 104 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRN---NIAKRHDEFERHAEGTFT 104 +\| +\|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|#+ \|\|+ + \|+ + + \|\|\|+\|+\|+ Sbjct 93 DEFERHAEGTFTSDVSSYLEGQAAKEF#IAWLVKGRGRRDFPEEVTIVEELRRRHADGSFS 152 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTK 82 +\|\|+ \|+\|+ + + \|\|+ +\|\|#+ \|\|++\|\| Sbjct 144 RRHADGSFSDEMNTVLDNLATRDF#INWLLHTK 175
Neoceratodus forsteri	Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \| \|\|\|\|\|\|\|\| \|\|\|+\| \|++\| \| \|\|#\|\| \|\|\|\|\|\|\| ++\|\|\| \| \|\|\|\|\|\|\|+\| Sbjct 20 EAKRHSQGTFMSDYAKLLEARHALDF#VQRLMNTKRN-GGVSKRHSEIERHAEGTYT 74 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEF-ERHAEGTFT 104 +\| +\|\|++\|\|+\|\|\| \| \|\|+ + \| +\|#\|+\|\|+ + \|+ +\| \|\|\|+\|\| \| Sbjct 63 SEIERHAEGTYTSDISSYLEGQAANEF#VRWLLKGRGRRDFSDTDAEEMGRRHADGTIT 120 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTK 82 +\|\|+ \|\| \|+\| + \|+\| ++\|#+ \|\|+\|+\| Sbjct 112 RRHADGTITNDMNNVLESIATREF#LNWLINSK 143
Canis lupus familiaris	Query 45 DQMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 45 DQMNEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNKNNIAKRHDEFERHAEGTFT 104 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRN---NIAKRHDEFERHAEGTFT 104 +\| +\|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|#+ \|\|+ + \|+ +\| + \|\|\|+\|+\|+ Sbjct 93 DEFERHAEGTFTSDVSSYLEGQAAKEF#IAWLVKGRGRRDFPEEVAIVEEFRRRHADGSFS 152 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTK 82 +\|\|+ \|+\|+ + + \|\|+ +\|\|#+ \|\|+ \|\| Sbjct 144 RRHADGSFSDEMNTVLDTLATRDF#INWLLQTK 175
Contains: Glucagon; Glucagon-36 (Oxyntomodulin); Glucagon-like peptide	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|+ \| \|\|+\|\|+\| Sbjct 1 HSQGTFTNDYSKYLDTRRAQDF#VQWLMSTKRS-GGITXXXXXXXXHADGTYT 51 Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNR 85 \|+ \|\|+\|\|\| \| \|\| + \|+ \|#\| \|\| + \| Sbjct 45 HADGTYTSDVSSYLQDQAAKKF#VTWLKQGQDRR 77
Hydrolagus colliei	Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|\|\|+ \| \|+\|\|\|\|\|\|\|\|+\|\| +\|\|#\|\|\|\|++\|\|\|\| \| \|+ +\|\|\|+\| +\| Sbjct 30 KRHTDGIFSSDYSKYLDNRRTKDF#VQWLLSTKRNGANT----DKTKRHADGIYT 79 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNN--IAKRHDEFERHAE 100 ++ \|\|\|+ \| +\|\|\| + \| +++ \|#\|+ \| \| + +\|+ ++\|\|\| \| \| \| Sbjct 68 DKTKRHADGIYTSDVASLTDYLKSKRF#VESLSNYNKRQNDRRMSKRHVEGARRTE 122 Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFE 96 \| \| ++ + +\|+\| \|\|+++ \|+\|\|#+ \|\|+ + \| + \|+ \| \| Sbjct 116 EGARRTEEDYPNDFSSYLEAKAAKDF#IDWLIK-GQGRRDFAEESREIE 162
Heloderma suspectum, venom, Peptide, 39 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMN 80 \| +\|\|\|\|\|\| \|\| ++ + \|#++\|\| \| Sbjct 1 HGEGTFTSDLSKQMEEEAVRLF#IEWLKN 28
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 33 aa	Query 56 GTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRN 86 \|\|+\|\|\| \| \|\| + \|++\|#\| \|\| + \|+ Sbjct 3 GTYTSDVSSYLQDQAAKEF#VSWLKTGRGRRD 33
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 34 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNR 85 \|+ \|\| \|\|\| \| +\|+ + ++\|#+ \|\|++ + \| Sbjct 1 HADGTLTSDISSFLEKQATKEF#IAWLVSGRGRR 33
Agkistrodon piscivorus	Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNIAKRH---DEFERHAEGTFT 104 \| +\|\|+ \|\|+\|\|\| \| \|\|+ + \|++\|#+ \|\|+\| + \|+ \| \| \|\|\|+\|\|\|\| Sbjct 32 EYERHADGTYTSDISSYLEGQAAKEF#IAWLVNGRGRRDFSEGAHTGEDLGRRHADGTFT 90 Query 51 KRHSQGTFTSDYSKYLDSRRAQDF#VQWLMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\|\|+\| \|\| \|+\|#++\|\|+\| \| + ++ Sbjct 82 RRHADGTFTSDYNKLLDDIATQEF#LKWLINQKVTQRDL 119 Query 66 LDSRRAQDF#VQWLMNTKRNRNNIAKRHDEFERHAEGTFT 104 \|+ \| ++\|#+ \| + \| +\|+ \| \|+\|\|\|\|+\|\|+\| Sbjct 9 LEDREKENF#LDQLAS-----NGLARPHAEYERHADGTYT 42

[Site 2] SDYSKYLDSR⁶⁹-RAQDFVQWLM

Arg⁶⁹ Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ser⁶⁰	Asp⁶¹	Tyr⁶²	Ser⁶³	Lys⁶⁴	Tyr⁶⁵	Leu⁶⁶	Asp⁶⁷	Ser⁶⁸	Arg⁶⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg⁷⁰	Ala⁷¹	Gln⁷²	Asp⁷³	Phe⁷⁴	Val⁷⁵	Gln⁷⁶	Trp⁷⁷	Leu⁷⁸	Met⁷⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
PLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHA

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	130.00	10	glucagon, isoform CRA_b
2	Pan troglodytes	102.00	2	PREDICTED: fibroblast activation protein, alpha su
3	N/A	79.70	44	0
4	Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 33 aa	64.70	1	AAB31092.1
5	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 29 aa	61.60	1	glucagon=proglucagon-derived 3.5 kda peptide
6	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 32 aa	60.80	1	glucagon II
7	Contains: Glucagon; Glucagon-like peptide	60.10	4	GLUC_AMICA Glucagon precursor
8	Amia calva=bowfin, pancreatic tissues, Peptide, 29 aa	60.10	1	glucagon
9	Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 29 aa	58.50	1	glucagon G-29
10	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 36 aa	57.80	1	GLUC1_ORENI Glucagon-1 (Glucagon I) gb
11	Lampetra fluviatilis=river lamprey, small intestine, Peptide, 29 aa	56.20	1	GLUC_LAMFL Glucagon gb
12	deshis1, Desphe6, Glu9	53.50	1	A Chain A, Nmr Solution Structure Of The Glucagon
13	Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 29 aa	53.50	1	glucagon
14	Contains: Glucagon-1 (Glucagon I); Glucagon-like peptide 1-I (GLP-1I); Glucagon-like peptide 2-I (GLP-2I)	47.00	1	GLUC1_PETMA Glucagon-1 precursor
15	Agkistrodon piscivorus	45.10	1	proglucagon
16	Gallus gallus	44.30	4	glucagon isoform 1
17	Meleagris gallopavo	44.30	2	preproglucagon B
18	Monodelphis domestica	43.90	3	PREDICTED: similar to Gastric inhibitory polypepti
19	Oncorhynchus tshawytscha	43.50	1	S78474_1 proglucagon
20	Rattus norvegicus	42.70	5	Glucose-dependent insulinotropic peptide
21	Canis familiaris	42.70	2	PREDICTED: similar to Gastric inhibitory polypepti
22	Xenopus laevis	42.40	5	proglucagon I
23	Xenopus tropicalis	42.40	2	glucagon
24	Danio rerio	42.00	5	hypothetical protein LOC494052
25	Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide 1 (GLP-1); Glucagon-like peptide 1(7-37) (GLP-1(7-37)); Glucagon-like peptide 1(7-36) (GLP-1(7-36)); Glucagon-like peptide 2 (GLP-2)	42.00	1	GLUC_HELSU Glucagon precursor
26	Heloderma suspectum	41.60	2	proglucagon
27	Hoplobatrachus rugulosus	41.60	1	AF324209_1 proglucagon
28	Contains: Glicentin-related polypeptide (GRPP); Glucagon-2 (Glucagon II); Glucagon-like peptide 2 (Glucagon-like peptide II)	41.20	1	GLUC2_LOPAM Glucagon-2 precursor (Glucagon II)
29	Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 32 aa	41.20	1	glucagon-like peptide, GLP
30	Macaca mulatta	40.80	3	PREDICTED: gastric inhibitory polypeptide
31	Bos taurus	40.40	5	PREDICTED: similar to Gastric inhibitory polypepti
32	Ictalurus punctatus	40.40	3	proglucagon I
33	Protopterus dolloi	40.40	1	proglucagon
34	Sebastes caurinus	40.00	4	proglucagon II
35	Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide	40.00	2	GLUC_CARAU Glucagon precursor
36	Tetraodon nigroviridis	40.00	2	unnamed protein product
37	Heloderma horridum	40.00	1	EXE3_HELHO Exendin-3 precursor emb
38	Contains: Glicentin-related polypeptide 1 (GRPP 1); Glucagon-1; Glucagon-like peptide 1-1 (GLP 1-1); Glucagon-like peptide 2 (GLP 2)	40.00	1	GLUC1_ONCMY Glucagon-1 precursor (Glucagon I)
39	Contains: Glicentin-related polypeptide 2 (GRPP 2); Glucagon-2; Glucagon-like peptide 1-2 (GLP 1-2); Glucagon-like peptide 2 (GLP 2)	40.00	1	GLUC2_ONCMY Glucagon-2 precursor (Glucagon II)
40	Mus musculus	39.70	6	gastric inhibitory polypeptide
41	Neoceratodus forsteri	39.70	1	proglucagon
42	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 33 aa	38.90	1	glucagon-like peptide-2, GLP-2=proglucagon-derived
43	Contains: Glucagon-2 (Glucagon II) (Glu II); Glucagon-like peptide 2-II (GLP-2II)	38.10	1	GLUC2_PETMA Glucagon-2 precursor
44	synthetic construct	37.00	1	Homo sapiens glucagon
45	Contains: Glicentin-related polypeptide (GRPP); Glucagon-1 (Glucagon I); Glucagon-like peptide 1 (Glucagon-like peptide I)	36.60	1	GLUC1_LOPAM Glucagon-1 precursor (Glucagon I)
46	Phodopus sungorus	36.60	1	glucagon precursor
47	Sus scrofa	36.20	1	glucagon
48	Contains: Glucagon; Glucagon-36 (Oxyntomodulin); Glucagon-like peptide	35.80	1	GLUC_LEPSP Glucagon precursor
49	Canis lupus familiaris	35.80	1	glucagon
50	Squalus acanthias	35.40	1	proglucagon
51	Hydrolagus colliei	35.40	1	proglucagon
52	Heloderma suspectum, venom, Peptide, 39 aa	35.40	1	A Chain A, Solution Structure Of Exendin-4 In 30-V
53	Bufo marinus	35.40	1	proglucagon
54	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 34 aa	34.70	1	glucagon-like peptide-1, GLP-1=proglucagon-derived
55	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 33 aa	34.70	1	GLUC2_ORENI Glucagon-2 (Glucagon II) gb
56	Capra hircus	34.30	1	glucagon
57	Contains: Intestinal peptide PHI-42; Intestinal peptide PHI-27 (Peptide histidine isoleucinamide 27); Vasoactive intestinal peptide (VIP) (Vasoactive intestinal polypeptide)	33.50	1	VIP_MOUSE VIP peptides precursor
58	Arvicanthis ansorgei	32.70	1	vasoactive intestinal polypeptide
59	Candida glabrata	32.70	1	unnamed protein product
60	Ambloplites rupestris	32.30	1	proglucagon

Top-ranked sequences

organism	matching
Homo sapiens	Query 40 PLSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 40 PLSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99
Pan troglodytes	Query 40 PLSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDE 94 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| +++\| \|\| \| Sbjct 962 PLSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRN-SSVAYRHAE 1015
N/A	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIA 89 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 1 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNKNNIA 37
Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 33 aa	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRN 84 \|\|+\|\|\|\|\|\|\|\|\|\|+\|+\|#\|\|+\|\|\|\|\|\|\|+\|\|\|\| Sbjct 1 HSEGTFTSDYSKYMDNR#RAKDFVQWLMSTKRN 32
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNT 81 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|#\|\|\|\|\|+\|\|\|\|+\| Sbjct 1 HSQGTFTSDYSKYLDNR#RAQDFIQWLMST 29
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 32 aa	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRN 84 \|\|+\|\|\|++\|\|\|\|\|\|++\|#\|\|\|\|\|\|\|\|\| \|+\|\|\| Sbjct 1 HSEGTFSNDYSKYLETR#RAQDFVQWLKNSKRN 32
Contains: Glucagon; Glucagon-like peptide	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNT 81 \|\|\|\|\|\|\|+\|\|\|\|\|+\|+\|#\|\|\|\|\|\|\|\|\|\|+\| Sbjct 1 HSQGTFTNDYSKYMDTR#RAQDFVQWLMST 29
Amia calva=bowfin, pancreatic tissues, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNT 81 \|\|\|\|\|\|\|+\|\|\|\|\|+\|+\|#\|\|\|\|\|\|\|\|\|\|+\| Sbjct 1 HSQGTFTNDYSKYMDTR#RAQDFVQWLMST 29
Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNT 81 \|\|+\|\|\|\|\|\|\|\|\|\|+\|+\|#\|\|+\|\|\|\|\|\|\|+\| Sbjct 1 HSEGTFTSDYSKYMDNR#RAKDFVQWLMST 29
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 36 aa	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRN 84 \|\|+\|\|\|++\|\|\|\|\|\|+ \|#+\|\|\|\|\|+\|\|\|\| \|\|+ Sbjct 1 HSEGTFSNDYSKYLEDR#KAQDFVRWLMNNKRS 32
Lampetra fluviatilis=river lamprey, small intestine, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNT 81 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|+#+\|+\|\|\| \|\|\|\|\| Sbjct 1 HSQGSFTSDYSKYLDSK#QAKDFVIWLMNT 29
deshis1, Desphe6, Glu9	Query 54 SQGTFTSDYSKYLDSR#RAQDFVQWLMNT 81 \|\|\|\| \|\|+\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 SQGT-TSEYSKYLDSR#RAQDFVQWLMNT 27
Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMN 80 \|\|+\|\|\|\|\|\|\|\|\|\|\|+++#+\|+\|\|\|+\|\|\|\| Sbjct 1 HSEGTFTSDYSKYLENK#QAKDFVRWLMN 28
Contains: Glucagon-1 (Glucagon I); Glucagon-like peptide 1-I (GLP-1I); Glucagon-like peptide 2-I (GLP-2I)	Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRH 92 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|+++#+\|+\|\|\|+\|\|\|\| \|\| + + +\|\| Sbjct 41 KRHSEGTFTSDYSKYLENK#QAKDFVRWLMNAKRGGSELQRRH 82 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNR---------NNIAKRHDE 94 +\|\|+ \|\|\|\|+\| + \|\|\|++# \|+\|\|\| \|\| + ++\| \|+ \|\|\| \| Sbjct 80 RRHADGTFTNDMTSYLDAK#AARDFVSWLARSDKSRRDGGDHLAENSEDKRHAE 132
Agkistrodon piscivorus	Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\|\|+\| \|\| # \|+\|++\|\|+\| \| + ++ Sbjct 82 RRHADGTFTSDYNKLLDDI#ATQEFLKWLINQKVTQRDL 119 Query 49 EDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRH---DEFERHA 99 \| +\|\|+ \|\|+\|\|\| \| \|\|+ +# \|++\|+ \|\|+\| + \|+ \| \| \|\|\| Sbjct 32 EYERHADGTYTSDISSYLEGQ#AAKEFIAWLVNGRGRRDFSEGAHTGEDLGRRHA 85
Gallus gallus	Query 40 PLSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRN--------------- 84 \|\| + \|+\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 42 PLDESRQLNEVKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMSTKRNGQQGQEDKENDKFPD 101 Query 85 ---RNNIAKRHDEFERHA 99 \| \|+\|\|\| \|\|\|\|\|\| Sbjct 102 QLSSNAISKRHSEFERHA 119 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\| +\| \|\| # \|++\|++\|\|+\|\|\| + ++ Sbjct 164 RRHADGTFTSDINKILDDM#AAKEFLKWLINTKVTQRDL 201 Query 48 NEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRN 86 +\| +\|\|++\|\|+\|\|\| + \|\|+ +# \|++\|+ \|\|+\| + \|+ Sbjct 113 SEFERHAEGTYTSDITSYLEGQ#AAKEFIAWLVNGRGRRD 151
Meleagris gallopavo	Query 40 PLSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRN--------------- 84 \|\| + \|+\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 42 PLDESRQLNEVKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMSTKRNGQQGQEDKENDEFPD 101 Query 85 ---RNNIAKRHDEFERHA 99 \| \|+\|\|\| \|\|\|\|\|\| Sbjct 102 QLSSNAISKRHSEFERHA 119 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\| +\| \|\| # \|++\|++\|\|+\|\|\| + ++ Sbjct 164 RRHADGTFTSDINKILDDM#AAKEFLKWLINTKVTQRDL 201 Query 48 NEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRN 86 +\| +\|\|++\|\|+\|\|\| + \|\|+ +# \|++\|+ \|\|+\| + \|+ Sbjct 113 SEFERHAEGTYTSDITSYLEGQ#AAKEFIAWLVNGRGRRD 151
Monodelphis domestica	Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 +\|+++\|\|\| \|\|\|\| +\| # \|\|\|\| \|\|++ \| +\| + \|\|+ \|\| \| Sbjct 51 QRYAEGTFISDYSITMDKI#MQQDFVNWLLSQKGKKN--SWRHNITERKA 97
Oncorhynchus tshawytscha	Query 54 SQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRH 92 \| \|\|\|++\|\|\|\|\| + \|# \|\|\|\|\|\|\|\|\|\|+\|\|+ +\|\|\| Sbjct 2 SXGTFSNDYSKYQEER#MAQDFVQWLMNSKRS-GAPSKRH 39 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNR 85 \|\|\|+ \|\|+\|\|\| \| \|\| +# \|+\|\|\| \|\| + + \| Sbjct 37 KRHADGTYTSDVSTYLQDQ#AAKDFVSWLKSGRARR 71
Rattus norvegicus	Query 52 RHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 \|+++\|\|\| \|\|\|\| +\| #\| \|\|\|\| \|\|+ \| +\|+ +\|+ +\| \| Sbjct 43 RYAEGTFISDYSIAMDKI#RQQDFVNWLLAQKGKKND--WKHNLTQREA 88
Canis familiaris	Query 52 RHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 \|+++\|\|\| \|\|\|\| +\| #\| \|\|\|\| \|\|+ \| +\|+ +\|+ +\| \| Sbjct 43 RYAEGTFISDYSIAMDKI#RQQDFVNWLLAQKGKKND--WKHNITQREA 88
Xenopus laevis	Query 41 LSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 + \| +\|+ \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|+ +++\|+ ++\|\|\|\| Sbjct 41 VDDSEQLKEVKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRS-GELSRRNADYERHA 98 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 +\|\|++\|\|\|\|\|\| ++ \|\| +# \|++\|+ \|\|+\| ++ \|++\|+ \|\|\|\|\|\| Sbjct 95 ERHAEGTFTSDVTQQLDEK#AAKEFIDWLINGGPSKEIISRRNAEFERHA 143 Query 52 RHSQGTFTSDYSKYLDSR#RAQDFVQWLM--NTKRNRNNIAKRHDEFERHA 99 \|\|++\|\|\|\|+\| + \|\|+ +# \|++\|\| \|\|+ \|\|\| + + + \|\|\| Sbjct 179 RHAEGTFTNDMTNYLEEK#AAKEFVGWLIKGRPKRNFSEVHSVEEMDRRHA 228 Query 50 DKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNI 88 \|+\|\|+ \|+\|\|+\| +\| \|\| # \|\|+\|+ \|++\|\|+ ++ Sbjct 224 DRRHADGSFTNDINKVLDII#AAQEFLDWVINTQETERDL 262 Query 49 EDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDE 94 \| +\|\|++\|\|+\|+\| ++\|\|+ +# \|++\|++\|\|+ \| + + \|\| \| Sbjct 138 EFERHAEGTYTNDVTEYLEEK#AAKEFIEWLIKGKPKKIRYS-RHAE 182
Xenopus tropicalis	Query 41 LSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 + \| \|+ \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\|\|+ +++\|+ ++\|\|\|\| Sbjct 41 VDDSQQLKEVKRHSQGTFTSDYSKYLDSR#RAQDFIQWLMNTKRS-GGLSRRNADYERHA 98 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 +\|\|++\|\|\|\|\|\| +++\|\| +# \|++\|+ \|\|+\| ++ \|++\|+ + \|\|\|\| Sbjct 95 ERHAEGTFTSDVTQHLDEK#AAKEFIDWLINGGPSKEIISRRNADVERHA 143 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDE 94 +\|\|++\|\|+\|+\| ++\|\|+ +# \|++\|++\|\|+\| \| + + \|\| \| Sbjct 140 ERHAEGTYTNDVTEYLEEK#AAKEFIEWLINGKPKKIRYS-RHAE 182 Query 52 RHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRN 86 \|\|++\|\|\|\|+\| + \|\|+ +# \|++\|\| \|\|+ + \|\| Sbjct 179 RHAEGTFTNDMTNYLEEK#AAKEFVGWLIKGRPKRN 213 Query 50 DKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTK 82 \|+\|\|+ \|+\|\|+\| +\| \|\| # \|\|+\|+ \|++\|\|+ Sbjct 224 DRRHADGSFTNDINKVLDII#AAQEFLDWVINTQ 256
Danio rerio	Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDE 94 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|#\|\|\|\|\|\|\|\|\|\|\| \|\|\| ++ \|\|\| \| Sbjct 48 KRHSEGTFSNDYSKYLETR#RAQDFVQWLMNAKRNGGSV-KRHAE 90 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWL 78 \|\|\|++\|\|+\|\|\| \| \|\| +# \|\| \|\| \|\| Sbjct 86 KRHAEGTYTSDVSSYLQDQ#AAQSFVAWL 113
Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide 1 (GLP-1); Glucagon-like peptide 1(7-37) (GLP-1(7-37)); Glucagon-like peptide 1(7-36) (GLP-1(7-36)); Glucagon-like peptide 2 (GLP-2)	Query 40 PLSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKR---------------- 83 \|\| \| \|+\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 40 PLDDSRQLNEVKRHSQGTFTSDYSKYLDTR#RAQDFVQWLMNTKRSGQQGVEEREKENLLD 99 Query 84 --NRNNIAKRHDEFERHA 99 + \| +\|+ \| \|+\|\|\|\| Sbjct 100 QLSSNGLARHHAEYERHA 117 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\|\|++ \|\| # \|+\|++\|\|+\| \| + ++ Sbjct 162 RRHADGTFTSDYNQLLDDI#ATQEFLKWLINQKVTQRDL 199 Query 49 EDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRN 86 \| +\|\|+ \| +\|\|\| \| \|\|+ +# \|++\|+ \|\|+\| + \|+ Sbjct 112 EYERHADGRYTSDISSYLEGQ#AAKEFIAWLVNGRGRRD 149
Heloderma suspectum	Query 40 PLSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKR---------------- 83 \|\| \| \|+\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 40 PLDDSRQLNEVKRHSQGTFTSDYSKYLDTR#RAQDFVQWLMNTKRSGQQGVEEREKENLLD 99 Query 84 --NRNNIAKRHDEFERHA 99 + \| +\|+ \| \|+\|\|\|\| Sbjct 100 QLSSNGLARHHAEYERHA 117 Query 49 EDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNR 85 \| +\|\|+ \| +\|\|\| \| \|\|+ +# \|++\|+ \|\|+\| + \| Sbjct 112 EYERHADGRYTSDISSYLEGQ#AAKEFIAWLVNGRGRR 148
Hoplobatrachus rugulosus	Query 41 LSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 \| \| \| \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|+\|\|+ \|+\|\|+ +\|\|\|\|\| Sbjct 41 LDDSDHHNEVKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNSKRS-GGISKRNVQFERHA 98 Query 50 DKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNT 81 \|+\|\|+ \|+\|\|\|\|++\| \|\| +# \|\|+\|+ \|++\|\| Sbjct 178 DRRHADGSFTSDFNKALDIK#AAQEFLDWIINT 209 Query 52 RHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRN 86 \|\|++\|\|\|\|\|\| + \|\|+ +# \|++\|\| \|\|+ + \|\| Sbjct 134 RHAEGTFTSDMTSYLEEK#AAKEFVDWLIKGRPKRN 168 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDE 94 +\|\|++\|\|+\|+\| +++\|+ +# \|++\|+ \|\|+ \| + ++ \|\| \| Sbjct 95 ERHAEGTYTNDVTQFLEEK#AAKEFIDWLIKGKPKKQRLS-RHAE 137
Contains: Glicentin-related polypeptide (GRPP); Glucagon-2 (Glucagon II); Glucagon-like peptide 2 (Glucagon-like peptide II)	Query 41 LSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRH 92 \|++\| + \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|#\|\|\|\|\|\|\|\|\| \|+\| \|\| + +\|\| Sbjct 40 LTEPIEPLNMKRHSEGTFSNDYSKYLETR#RAQDFVQWLKNSK--RNGLFRRH 89 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWL 78 +\|\|+ \|\|+\|\|\| \| \|\| +# \|+\|\|\| \|\| Sbjct 87 RRHADGTYTSDVSSYLQDQ#AAKDFVSWL 114
Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 32 aa	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRN 84 \|+ \|\|\|\|+\| + \|\|\|++# \|+\|\|\| \|\| + ++ Sbjct 1 HADGTFTNDMTSYLDAK#AARDFVSWLARSDKS 32
Macaca mulatta	Query 52 RHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 \|+++\|\|\| \|\|\|\| +\| # \|\|\|\| \|\|+ \| +\|+ +\|+ +\| \| Sbjct 51 RYAEGTFISDYSIAMDKI#HQQDFVNWLLAQKGKKND--WKHNITQREA 96
Bos taurus	Query 52 RHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNN 87 \|+++\|\|\| \|\|\|\| +\| #\| \|\|\|\| \|\|+ \| +++ Sbjct 81 RYAEGTFISDYSIAMDKI#RQQDFVNWLLAQKGKKSD 116
Ictalurus punctatus	Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRH 92 +\|\|\|+\|\|\|++\|\|\|\|\|\|++\|#\|\|\|\|\|\|\|\|\|\|\|+\|\|+ ++ \|+\|\| Sbjct 49 RRHSEGTFSNDYSKYLETR#RAQDFVQWLMNSKRS-DSPARRH 89 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRH 92 +\|\| \|+\|\|\|\| +\| \|\|\| # \|++++\|\|+\|\|+ + + \|\|\| Sbjct 134 RRHVDGSFTSDVNKVLDSL#AAKEYLQWVMNSPSSAS--GKRH 173 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWL 78 +\|\|+ \|\|+\|\|\| \| \|\| +# \|+\|\|+ \|\| Sbjct 87 RRHADGTYTSDVSSYLQDQ#AAKDFITWL 114
Protopterus dolloi	Query 49 EDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 \| \|\|\|\|\|\|\|\| \|\|\|+\| \|+++# \| \| \|\| \|\|+\|\|\| ++\|\|\|+\| \|\|\|\| Sbjct 28 EAKRHSQGTFMSDYAKLLEAK#HALDLVQRLMSTKRT-GAVSKRHNEIERHA 77 Query 48 NEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFER 97 \|\| +\|\|++\|\|+\|\|\| \| \|\|+ +# +\|++\|\|\| + \|+ +\| +\| Sbjct 71 NEIERHAEGTYTSDISSYLEGQ#AVNEFIRWLMKGRGRRDFSETDIEEMDR 120 Query 42 SDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTK 82 \|+ \| \|+\|\|+ \|\| \|\|\| + \|+\| # ++\|+ \|\|+\| \| Sbjct 111 SETDIEEMDRRHADGTVTSDINSVLESI#ATREFLNWLINAK 151
Sebastes caurinus	Query 41 LSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRH 92 \|++\| ++ \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|#\|\|\|\|\|\|\|\|\| \|+\|\|\| ++ +\|\| Sbjct 40 LAEPIELPNMKRHSEGTFSNDYSKYLETR#RAQDFVQWLKNSKRN-GSLFRRH 90 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNR 85 +\|\|+ \|\|+\|\|\| \| \|\| +# \|++\|\| \|\| + \| Sbjct 88 RRHADGTYTSDVSSYLQDQ#AAKEFVYWLKTGRGRR 122
Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide	Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDE 94 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|#\|\|\|\|\|\|+\|\|\|\|+\| \| + \|\|\|\| \| Sbjct 48 KRHSEGTFSNDYSKYLETR#RAQDFVEWLMNSKSNGGS-AKRHAE 90 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWL 78 \|\|\|++\|\|+\|\|\| \| +\| +# \|\|+\|\| \|\| Sbjct 86 KRHAEGTYTSDISSFLRDQ#AAQNFVAWL 113
Tetraodon nigroviridis	Query 41 LSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRH 92 \|++\| ++ \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|#\|\|\|\|\|\|\|\|\| \|+\|\|\| ++ +\|\| Sbjct 777 LTEPSDLSNMKRHSEGTFSNDYSKYLETR#RAQDFVQWLKNSKRN-GSLFRRH 827 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNR 85 +\|\|+ \|\|+\|\|\| \| \|\| +# \|++\|\| \|\| + \| Sbjct 825 RRHADGTYTSDVSAYLQDQ#AAKEFVSWLKTGRGRR 859
Heloderma horridum	Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMN 80 \|\|\|\| \|\|\|\|\|\| \|\| ++ # + \|++\|\| \| Sbjct 46 KRHSDGTFTSDLSKQMEEE#AVRLFIEWLKN 75
Contains: Glicentin-related polypeptide 1 (GRPP 1); Glucagon-1; Glucagon-like peptide 1-1 (GLP 1-1); Glucagon-like peptide 2 (GLP 2)	Query 41 LSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRH 92 \| \| ++ \|\|\|\|+\|\|\|++\|\|\|\|\| + \|# \|\|\|\|\|\|\|\|\|\|+\|\|+ +\|\|\| Sbjct 40 LEDLMGVSNVKRHSEGTFSNDYSKYQEER#MAQDFVQWLMNSKRS-GAPSKRH 90 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNN 87 \|\|\|+ \|\|+\|\|\| \| \|\| +# \|+\|\|\| \|\| + + \| + Sbjct 88 KRHADGTYTSDVSTYLQDQ#AAKDFVSWLKSGRARRES 124 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHD 93 +\|\| \|+\|\|\|\| +\| \|\|\| # \|++++ \|+\| +\| + + ++ \| Sbjct 135 RRHVDGSFTSDVNKVLDSL#AAKEYLLWVMTSKTSGKSNKRQED 177
Contains: Glicentin-related polypeptide 2 (GRPP 2); Glucagon-2; Glucagon-like peptide 1-2 (GLP 1-2); Glucagon-like peptide 2 (GLP 2)	Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRH 92 \|\| \|+\|\|\|++ \|\|\|\| + \|# \|+\|\|+ \|\|\|\|+\|\|+ +\|\|\| Sbjct 50 KRQSEGTFSNYYSKYQEER#MARDFLHWLMNSKRS-GAPSKRH 90 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNN 87 \|\|\|+ \|\|+\|\|\| \| \|\| +# \|+\|\|\| \|\| + \| + Sbjct 88 KRHADGTYTSDVSTYLQDQ#AAKDFVSWLKSGPARRES 124 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHD 93 +\|\| \|+\|\|\|\| +\| \|\|\| # \|++++ \|+\| +\| + + ++ \| Sbjct 135 RRHVDGSFTSDVNKVLDSL#AAKEYLLWVMTSKTSGKSNKRQED 177
Mus musculus	Query 52 RHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 \|+++\|\|\| \|\|\|\| +\| #\| \|\|\|\| \|\|+ + +++ +\|+ +\| \| Sbjct 43 RYAEGTFISDYSIAMDKI#RQQDFVNWLLAQRGKKSD--WKHNITQREA 88
Neoceratodus forsteri	Query 40 PLSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 \|+ + \| \|\|\|\|\|\|\|\| \|\|\|+\| \|++\|# \| \|\|\|\| \|\|\|\|\|\|\| ++\|\|\| \| \|\|\|\| Sbjct 11 PVDASREHTEAKRHSQGTFMSDYAKLLEAR#HALDFVQRLMNTKRN-GGVSKRHSEIERHA 69 Query 48 NEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRN 86 +\| +\|\|++\|\|+\|\|\| \| \|\|+ +# \| +\|\|+\|\|+ + \|+ Sbjct 63 SEIERHAEGTYTSDISSYLEGQ#AANEFVRWLLKGRGRRD 101 Query 42 SDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTK 82 \|\| \| +\|\|+ \|\| \|+\| + \|+\| # ++\|+ \|\|+\|+\| Sbjct 103 SDTDAEEMGRRHADGTITNDMNNVLESI#ATREFLNWLINSK 143
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 33 aa	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNTK 82 \|+ \|+\|\|\|\| +\| \|\|+ # \|++\|+ \|\|++\|\| Sbjct 1 HADGSFTSDINKVLDTI#AAKEFLNWLISTK 30
Contains: Glucagon-2 (Glucagon II) (Glu II); Glucagon-like peptide 2-II (GLP-2II)	Query 52 RHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRN------RNNIAKRHDE 94 \|\|\|\|\|+\|\|\|\|\|\|\|+\|\| +#+\|+\|\|\| \|\|+\|\|\|\| \|+ \|\|\| + Sbjct 43 RHSQGSFTSDYSKHLDVK#QAKDFVTWLLNTKRRGVDAQAGANLEKRHSD 91 Query 48 NEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKR 83 \| +\|\|\|\| \|+\|\|+\| + \|\| # \|++\|++\|\| \| Sbjct 84 NLEKRHSDGSFTNDMNVMLDRM#SAKNFLEWLKQQGR 119
synthetic construct	Query 40 PLSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 40 PLSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTK 82 +\|\|+ \|+\|+ + + \|\|+ # \|+\|\|+ \|\|+ \|\| Sbjct 144 RRHADGSFSDEMNTILDNL#AARDFINWLIQTK 175
Contains: Glicentin-related polypeptide (GRPP); Glucagon-1 (Glucagon I); Glucagon-like peptide 1 (Glucagon-like peptide I)	Query 43 DPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRH 92 +\| +++ \|\|\|\|+\|\|\|++\|\|\|\|\|\|+ \|#+\|\|+\|\|+\|\|\|\| \|\|+ \|\|\| Sbjct 43 EPRELSNMKRHSEGTFSNDYSKYLEDR#KAQEFVRWLMNNKRS-GVAEKRH 91 Query 50 DKRHSQGTFTSDYSKYLDSR#RAQDFVQWL 78 +\|\|\|+ \|\|\|\|\|\| \| \|\| +# +\|\|\| \| Sbjct 88 EKRHADGTFTSDVSSYLKDQ#AIKDFVDRL 116
Phodopus sungorus	Query 56 GTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRN 86 \|\|\|\|\|\| \| \|\|+ +# \|++\|+ \|\|+ + \|+ Sbjct 1 GTFTSDVSSYLEGQ#AAKEFIAWLVKGRGRRD 31 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTK 82 +\|\|+ \|+\|+ + + \|\|\| # +\|\|+ \|\|+ \|\| Sbjct 44 RRHADGSFSDEMNTILDSL#ATRDFINWLIQTK 75
Sus scrofa	Query 40 PLSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 \|\| \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 40 PLDDPDQMTEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNKNNIAKRHDEFERHA 99 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTK 82 +\|\|+ \|+\|+ + + \|\|+ # +\|\|+ \|\|++\|\| Sbjct 144 RRHADGSFSDEMNTVLDNL#ATRDFINWLLHTK 175
Contains: Glucagon; Glucagon-36 (Oxyntomodulin); Glucagon-like peptide	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRN 84 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|+ Sbjct 1 HSQGTFTNDYSKYLDTR#RAQDFVQWLMSTKRS 32 Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNR 85 \|+ \|\|+\|\|\| \| \|\| +# \|+ \|\| \|\| + \| Sbjct 45 HADGTYTSDVSSYLQDQ#AAKKFVTWLKQGQDRR 77
Canis lupus familiaris	Query 40 PLSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 40 PLNDLDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNKNNIAKRHDEFERHA 99 Query 48 NEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRN 86 +\| +\|\|++\|\|\|\|\|\| \| \|\|+ +# \|++\|+ \|\|+ + \|+ Sbjct 93 DEFERHAEGTFTSDVSSYLEGQ#AAKEFIAWLVKGRGRRD 131 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTK 82 +\|\|+ \|+\|+ + + \|\|+ # +\|\|+ \|\|+ \|\| Sbjct 144 RRHADGSFSDEMNTVLDTL#ATRDFINWLLQTK 175
Squalus acanthias	Query 44 PDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFE 96 \|++\| \|+\|\|+ \|\|\|\|\|+ + \|\|+ # \|++\|+ \|++\|+\| \| \| \| \| Sbjct 118 PEEM--DRRHADGTFTSEINIVLDTI#AAKEFLNWILNSK----TIQSRDSEIE 164 Query 48 NEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWL--MNTKRNRNNIAKRHDEFERH 98 ++ \|\|\|++\|\|+\|\|\| \| #+\|+ \|\| \| \| ++\| +\|+\|\|\| \| \|\| Sbjct 20 DKTKRHAEGTYTSDVDSLSDYF#KAKRFVDSLTSYNKRQNGRSISKRHVESTRH 72 Query 67 DSR#RAQDFVQWLMNTKRNRNNIAKRHDE 94 \|+\|#\|\|+\|\|\|\|\|\|\|+\|\|\|\| + \|\|\| \| Sbjct 1 DNR#RAKDFVQWLMSTKRNGDK-TKRHAE 27 Query 49 EDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNR 85 \| \|\|++\|++ \| \| \|\|+++# \|+\|\|+ \|\|+ + \| Sbjct 68 ESTRHTEGSYR-DISSYLEAK#AAKDFINWLIKGRGRR 103
Hydrolagus colliei	Query 41 LSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 +\|+\| + + \|\|\|+ \| \|+\|\|\|\|\|\|\|\|+\|#\| +\|\|\|\|\|\|++\|\|\|\| \| \|+ +\|\|\| Sbjct 20 VSNPSTVTDVKRHTDGIFSSDYSKYLDNR#RTKDFVQWLLSTKRNGANT----DKTKRHA 74 Query 48 NEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNN--IAKRHDEFER 97 ++ \|\|\|+ \| +\|\|\| + \| #+++ \|\|+ \| \| + +\|+ ++\|\|\| \| \| Sbjct 68 DKTKRHADGIYTSDVASLTDYL#KSKRFVESLSNYNKRQNDRRMSKRHVEGAR 119 Query 49 EDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFE 96 \| \| ++ + +\|+\| \|\|+++# \|+\|\|+ \|\|+ + \| + \|+ \| \| Sbjct 116 EGARRTEEDYPNDFSSYLEAK#AAKDFIDWLIK-GQGRRDFAEESREIE 162
Heloderma suspectum, venom, Peptide, 39 aa	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMN 80 \| +\|\|\|\|\|\| \|\| ++ # + \|++\|\| \| Sbjct 1 HGEGTFTSDLSKQMEEE#AVRLFIEWLKN 28
Bufo marinus	Query 52 RHSQGTFTSDYSKYLDSR#RAQDFVQWLM--NTKRNRNNIAKRHDEFERHA 99 \|\|++\|\|\|\|\|\| + +\|+ +# \|++\|\| \|\|+ \|\|\| ++++ \| \|\|\| Sbjct 62 RHAEGTFTSDMTSFLEEK#AAKEFVDWLIKGRPKRNFSDVSAADDMDRRHA 111 Query 50 DKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNT 81 \|+\|\|+ \|+\|\|\|\|++\| \|\| +# \|\|+\|+ \|++\|\| Sbjct 107 DRRHADGSFTSDFNKALDIK#AAQEFLDWIINT 138 Query 51 KRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDE 94 +\|\|++\|\|+\|+\| +++\|+ +# \|++\|+ \|\|+ + ++ \|\| \| Sbjct 23 ERHAEGTYTNDVTQFLEEK#AAKEFIDWLLKGIPKKQRLS-RHAE 65 Query 73 DFVQWLMNTKRNRNNIAKRHDEFERHA 99 \|\| \|\|\|\|\|+\|\|+ +++\|+ +\|\|\|\|\| Sbjct 1 DFAQWLMNSKRS-GGMSRRNVQFERHA 26
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 34 aa	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNR 85 \|+ \|\| \|\|\| \| +\|+ +# ++\|+ \|\|++ + \| Sbjct 1 HADGTLTSDISSFLEKQ#ATKEFIAWLVSGRGRR 33
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 33 aa	Query 56 GTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRN 86 \|\|+\|\|\| \| \|\| +# \|++\|\| \|\| + \|+ Sbjct 3 GTYTSDVSSYLQDQ#AAKEFVSWLKTGRGRRD 33
Capra hircus	Query 48 NEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNR 85 +\| +\|\|++\|\|\|\|\|\| \| \|\|+ +# \|++\|+ \|\|+ + \| Sbjct 8 DEFERHAEGTFTSDVSSYLEGQ#AAKEFIAWLVKGRGRR 45 Query 86 NNIAKRHDEFERHA 99 \|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 86 NNIAKRHDEFERHA 99
Contains: Intestinal peptide PHI-42; Intestinal peptide PHI-27 (Peptide histidine isoleucinamide 27); Vasoactive intestinal peptide (VIP) (Vasoactive intestinal polypeptide)	Query 47 MNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 ++ + \|\|+ \| \|\|\|\|\|\|+ \| # \|+ +++ \|+ \|\| ++\|++ +\|\|+ Sbjct 75 VSRNARHADGVFTSDYSRLLGQI#SAKKYLESLIG-KRISSSISEDPVPIKRHS 126
Arvicanthis ansorgei	Query 47 MNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRHDEFERHA 99 ++ + \|\|+ \| \|\|\|\|\|\|+ \| # \|+ +++ \|+ \|\| ++\| + +\|\|+ Sbjct 58 VSRNARHADGVFTSDYSRLLGQI#SAKKYLESLIG-KRISSSILEDPVPIKRHS 109
Candida glabrata	Query 41 LSDPDQMNEDKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAK 90 \|+\| \| \|\|+ \| \| \| \| + +\|+ # \|\| \|+ + \| +\| +\| Sbjct 351 LADGDDDEEDEEDSSSTSESSYDEEIDNE#EKQDIVEEEEDADENDDNFSK 400
Ambloplites rupestris	Query 53 HSQGTFTSDYSKYLDSR#RAQDFVQWLMNTKRNRNNIAKRH 92 \|\|\|\|\|\|\|+\|\|+ \|\|+ \|#+\|\|\|\|++\|\|\|\| \|\|+ \|\|\| Sbjct 1 HSQGTFTNDYTNYLEDR#QAQDFIRWLMNNKRS-GAAEKRH 39 Query 50 DKRHSQGTFTSDYSKYLDSR#RAQDFVQWLMN--------TKRNRNNIAKRHDE 94 +\|\|\|+ \|\|\|\| \| \| +# +\|\|\| \| + \| \| \| ++\|\| \| Sbjct 36 EKRHADGTFTDDASSDFYDQ#AIKDFVAKLKSGQDRREPETDRRREAFSRRHVE 88

[Site 3] SRRAQDFVQW⁷⁷-LMNTKRNRNN

Trp⁷⁷ Leu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ser⁶⁸	Arg⁶⁹	Arg⁷⁰	Ala⁷¹	Gln⁷²	Asp⁷³	Phe⁷⁴	Val⁷⁵	Gln⁷⁶	Trp⁷⁷

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Leu⁷⁸	Met⁷⁹	Asn⁸⁰	Thr⁸¹	Lys⁸²	Arg⁸³	Asn⁸⁴	Arg⁸⁵	Asn⁸⁶	Asn⁸⁷

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
NEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHAEGTFTSDV

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	127.00	10	glucagon, isoform CRA_b
2	N/A	79.70	45	0
3	Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 33 aa	64.70	1	AAB31092.1
4	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 29 aa	61.60	1	glucagon=proglucagon-derived 3.5 kda peptide
5	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 32 aa	60.80	1	glucagon II
6	Contains: Glucagon; Glucagon-like peptide	60.10	4	GLUC_AMICA Glucagon precursor
7	Amia calva=bowfin, pancreatic tissues, Peptide, 29 aa	60.10	1	glucagon
8	Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 29 aa	58.50	1	glucagon G-29
9	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 36 aa	57.80	1	GLUC1_ORENI Glucagon-1 (Glucagon I) gb
10	Lampetra fluviatilis=river lamprey, small intestine, Peptide, 29 aa	56.20	1	GLUC_LAMFL Glucagon gb
11	Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 29 aa	53.50	1	glucagon
12	deshis1, Desphe6, Glu9	53.50	1	A Chain A, Nmr Solution Structure Of The Glucagon
13	Monodelphis domestica	48.90	3	PREDICTED: similar to Gastric inhibitory polypepti
14	Contains: Glucagon-1 (Glucagon I); Glucagon-like peptide 1-I (GLP-1I); Glucagon-like peptide 2-I (GLP-2I)	48.90	1	GLUC1_PETMA Glucagon-1 precursor
15	Xenopus laevis	47.00	5	proglucagon II
16	Hoplobatrachus rugulosus	46.60	1	AF324209_1 proglucagon
17	Bufo marinus	46.60	1	proglucagon
18	Gallus gallus	46.20	4	glucagon isoform 1
19	Meleagris gallopavo	46.20	2	preproglucagon B
20	Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide 1 (GLP-1); Glucagon-like peptide 1(7-37) (GLP-1(7-37)); Glucagon-like peptide 1(7-36) (GLP-1(7-36)); Glucagon-like peptide 2 (GLP-2)	44.70	1	GLUC_HELSU Glucagon precursor
21	Capra hircus	43.90	1	glucagon
22	Oncorhynchus tshawytscha	43.50	1	S78474_1 proglucagon
23	Ictalurus punctatus	43.10	3	proglucagon I
24	Rattus norvegicus	42.70	6	Glucose-dependent insulinotropic peptide
25	Canis familiaris	42.70	2	PREDICTED: similar to Gastric inhibitory polypepti
26	Ambloplites rupestris	42.70	1	proglucagon
27	Pan troglodytes	42.40	2	PREDICTED: fibroblast activation protein, alpha su
28	Xenopus tropicalis	42.40	2	glucagon
29	Danio rerio	42.00	6	hypothetical protein LOC494052
30	Bos taurus	42.00	5	vasoactive intestinal peptide
31	Macaca mulatta	41.60	3	PREDICTED: vasoactive intestinal peptide
32	Heloderma suspectum	41.60	2	proglucagon
33	Squalus acanthias	41.60	1	proglucagon
34	Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 32 aa	41.20	1	glucagon-like peptide, GLP
35	Contains: Glicentin-related polypeptide (GRPP); Glucagon-2 (Glucagon II); Glucagon-like peptide 2 (Glucagon-like peptide II)	41.20	1	GLUC2_LOPAM Glucagon-2 precursor (Glucagon II)
36	Sebastes caurinus	40.80	4	proglucagon I
37	Tetraodon nigroviridis	40.00	3	unnamed protein product
38	Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide	40.00	2	GLUC_CARAU Glucagon precursor
39	Contains: Glicentin-related polypeptide 2 (GRPP 2); Glucagon-2; Glucagon-like peptide 1-2 (GLP 1-2); Glucagon-like peptide 2 (GLP 2)	40.00	1	GLUC2_ONCMY Glucagon-2 precursor (Glucagon II)
40	Contains: Glicentin-related polypeptide 1 (GRPP 1); Glucagon-1; Glucagon-like peptide 1-1 (GLP 1-1); Glucagon-like peptide 2 (GLP 2)	40.00	1	GLUC1_ONCMY Glucagon-1 precursor (Glucagon I)
41	Heloderma horridum	40.00	1	EXE3_HELHO Exendin-3 precursor emb
42	Mus musculus	39.70	6	Gastric inhibitory polypeptide
43	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 33 aa	38.90	1	glucagon-like peptide-2, GLP-2=proglucagon-derived
44	Protopterus dolloi	38.90	1	proglucagon
45	Contains: Intestinal peptide PHI-42; Intestinal peptide PHI-27 (Peptide histidine isoleucinamide 27); Vasoactive intestinal peptide (VIP) (Vasoactive intestinal polypeptide)	38.50	1	VIP_MOUSE VIP peptides precursor
46	Agkistrodon piscivorus	38.50	1	proglucagon
47	Contains: Glucagon-2 (Glucagon II) (Glu II); Glucagon-like peptide 2-II (GLP-2II)	38.10	1	GLUC2_PETMA Glucagon-2 precursor
48	Arvicanthis ansorgei	37.70	1	vasoactive intestinal polypeptide
49	synthetic construct	37.00	1	Homo sapiens glucagon
50	Contains: Glicentin-related polypeptide (GRPP); Glucagon-1 (Glucagon I); Glucagon-like peptide 1 (Glucagon-like peptide I)	36.60	1	GLUC1_LOPAM Glucagon-1 precursor (Glucagon I)
51	Phodopus sungorus	36.60	1	glucagon precursor
52	Neoceratodus forsteri	36.20	1	proglucagon
53	Sus scrofa	36.20	1	glucagon
54	Canis lupus familiaris	35.80	1	glucagon
55	Contains: Glucagon; Glucagon-36 (Oxyntomodulin); Glucagon-like peptide	35.80	1	GLUC_LEPSP Glucagon precursor
56	Hydrolagus colliei	35.40	1	proglucagon
57	Heloderma suspectum, venom, Peptide, 39 aa	35.40	1	A Chain A, Solution Structure Of Exendin-4 In 30-V
58	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 33 aa	34.70	1	GLUC2_ORENI Glucagon-2 (Glucagon II) gb
59	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 34 aa	34.70	1	glucagon-like peptide-1, GLP-1=proglucagon-derived

Top-ranked sequences

organism	matching
Homo sapiens	Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSD 106 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSD 106
N/A	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIA 89 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|+\|\|\|\| Sbjct 1 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNKNNIA 37
Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 33 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRN 84 \|\|+\|\|\|\|\|\|\|\|\|\|+\|+\|\|\|+\|\|\|\|\|#\|\|+\|\|\|\| Sbjct 1 HSEGTFTSDYSKYMDNRRAKDFVQW#LMSTKRN 32
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNT 81 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|+\|\|#\|\|+\| Sbjct 1 HSQGTFTSDYSKYLDNRRAQDFIQW#LMST 29
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 32 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRN 84 \|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|#\| \|+\|\|\| Sbjct 1 HSEGTFSNDYSKYLETRRAQDFVQW#LKNSKRN 32
Contains: Glucagon; Glucagon-like peptide	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNT 81 \|\|\|\|\|\|\|+\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|\|#\|\|+\| Sbjct 1 HSQGTFTNDYSKYMDTRRAQDFVQW#LMST 29
Amia calva=bowfin, pancreatic tissues, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNT 81 \|\|\|\|\|\|\|+\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|\|#\|\|+\| Sbjct 1 HSQGTFTNDYSKYMDTRRAQDFVQW#LMST 29
Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNT 81 \|\|+\|\|\|\|\|\|\|\|\|\|+\|+\|\|\|+\|\|\|\|\|#\|\|+\| Sbjct 1 HSEGTFTSDYSKYMDNRRAKDFVQW#LMST 29
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 36 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRN 84 \|\|+\|\|\|++\|\|\|\|\|\|+ \|+\|\|\|\|\|+\|#\|\|\| \|\|+ Sbjct 1 HSEGTFSNDYSKYLEDRKAQDFVRW#LMNNKRS 32
Lampetra fluviatilis=river lamprey, small intestine, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNT 81 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|++\|+\|\|\| \|#\|\|\|\| Sbjct 1 HSQGSFTSDYSKYLDSKQAKDFVIW#LMNT 29
Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMN 80 \|\|+\|\|\|\|\|\|\|\|\|\|\|++++\|+\|\|\|+\|#\|\|\| Sbjct 1 HSEGTFTSDYSKYLENKQAKDFVRW#LMN 28
deshis1, Desphe6, Glu9	Query 54 SQGTFTSDYSKYLDSRRAQDFVQW#LMNT 81 \|\|\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\| Sbjct 1 SQGT-TSEYSKYLDSRRAQDFVQW#LMNT 27
Monodelphis domestica	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGT 102 +\|+++\|\|\| \|\|\|\| +\| \|\|\|\| \|#\|++ \| +\| + \|\|+ \|\| \|+\|\| Sbjct 51 QRYAEGTFISDYSITMDKIMQQDFVNW#LLSQKGKKN--SWRHNITERKADGT 100
Contains: Glucagon-1 (Glucagon I); Glucagon-like peptide 1-I (GLP-1I); Glucagon-like peptide 2-I (GLP-2I)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|++++\|+\|\|\|+\|#\|\|\| \|\| + + + \|\|\|+\|\|\|\|+\|+ Sbjct 41 KRHSEGTFTSDYSKYLENKQAKDFVRW#LMNAKRGGSELQR------RHADGTFTNDM 91 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNR---NNIAKRHDEFERHAE 100 +\|\|+ \|\|\|\|+\| + \|\|\|++ \|+\|\|\| \|#\| + ++\| + + \| +\|\|\|\| Sbjct 80 RRHADGTFTNDMTSYLDAKAARDFVSW#LARSDKSRRDGGDHLAENSEDKRHAE 132
Xenopus laevis	Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|+ +++\|+ ++\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 49 EVKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRS-GGLSRRNADYERHAEGTFTSDV 106 Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \| +\|\|++\|\|+\|+\| ++\|\|+ + + \|++\|#\|+ \| + + \|\|\|\|\|\|\|\|+\|+ Sbjct 138 EIERHAEGTYTNDVTEYLEEKATKAFIEW#LIKGK-------PKKIRYSRHAEGTFTNDM 189 Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRH 92 \|\|++\|\|\|\|+\| + \|\|+ + \|++\|\| \|#\|+\| + \| ++ + \| Sbjct 179 RHAEGTFTNDMTNYLEEKAAKEFVGW#LINGRPKRKDLLEEH 219
Hoplobatrachus rugulosus	Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|+\|\|+ \|+\|\|+ +\|\|\|\|\|\|\|\|+\|+\|\| Sbjct 48 NEVKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNSKRS-GGISKRNVQFERHAEGTYTNDV 106 Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFE-RHAEGTFTSD 106 \|\|++\|\|\|\|\|\| + \|\|+ + \|++\|\| \|#\|+ + \|\| +\| + \|\|\|+\|+\|\|\|\| Sbjct 134 RHAEGTFTSDMTSYLEEKAAKEFVDW#LIKGRPKRNFPDISAEEMDRRHADGSFTSD 189 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 +\|\|++\|\|+\|+\| +++\|+ + \|++\|+ \|#\|+ \| + ++ \|\|\|\|\|\|\|\|\|\|+ Sbjct 95 ERHAEGTYTNDVTQFLEEKAAKEFIDW#LIKGKPKKQRLS-------RHAEGTFTSDM 144 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNT 81 \|+\|\|+ \|+\|\|\|\|++\| \|\| + \|\|+\|+ \|#++\|\| Sbjct 178 DRRHADGSFTSDFNKALDIKAAQEFLDW#IINT 209
Bufo marinus	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQW#LM--NTKRNRNNIAKRHDEFERHAEGTFTSD 106 \|\|++\|\|\|\|\|\| + +\|+ + \|++\|\| \|#\|+ \|\|\| ++++ \| \|\|\|+\|+\|\|\|\| Sbjct 62 RHAEGTFTSDMTSFLEEKAAKEFVDW#LIKGRPKRNFSDVSAADDMDRRHADGSFTSD 118 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 +\|\|++\|\|+\|+\| +++\|+ + \|++\|+ \|#\|+ \| \|+ \|\|\|\|\|\|\|\|\|\|+ Sbjct 23 ERHAEGTYTNDVTQFLEEKAAKEFIDW#LL------KGIPKK-QRLSRHAEGTFTSDM 72 Query 73 DFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\| \|\|#\|\|\|+\|\|+ +++\|+ +\|\|\|\|\|\|\|\|+\|+\|\| Sbjct 1 DFAQW#LMNSKRS-GGMSRRNVQFERHAEGTYTNDV 34 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNT 81 \|+\|\|+ \|+\|\|\|\|++\| \|\| + \|\|+\|+ \|#++\|\| Sbjct 107 DRRHADGSFTSDFNKALDIKAAQEFLDW#IINT 138
Gallus gallus	Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRN------------------RNNIA 89 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|+\|\|\|\| \| \|+ Sbjct 50 NEVKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMSTKRNGQQGQEDKENDKFPDQLSSNAIS 109 Query 90 KRHDEFERHAEGTFTSDV 107 \|\|\| \|\|\|\|\|\|\|\|\|+\|\|\|+ Sbjct 110 KRHSEFERHAEGTYTSDI 127 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAK---RHDEFERHAEGTFT 104 +\| +\|\|++\|\|+\|\|\| + \|\|+ + \|++\|+ \|#\|+\| + \|+ \| + \|\|\|+\|\|\|\| Sbjct 113 SEFERHAEGTYTSDITSYLEGQAAKEFIAW#LVNGRGRRDFPEKALMAEEMGRRHADGTFT 172 Query 105 SDV 107 \|\|+ Sbjct 173 SDI 175 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\| +\| \|\| \|++\|++\|#\|+\|\|\| + ++ Sbjct 164 RRHADGTFTSDINKILDDMAAKEFLKW#LINTKVTQRDL 201
Meleagris gallopavo	Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRN------------------RNNIA 89 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|+\|\|\|\| \| \|+ Sbjct 50 NEVKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMSTKRNGQQGQEDKENDEFPDQLSSNAIS 109 Query 90 KRHDEFERHAEGTFTSDV 107 \|\|\| \|\|\|\|\|\|\|\|\|+\|\|\|+ Sbjct 110 KRHSEFERHAEGTYTSDI 127 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAK---RHDEFERHAEGTFT 104 +\| +\|\|++\|\|+\|\|\| + \|\|+ + \|++\|+ \|#\|+\| + \|+ \| + \|\|\|+\|\|\|\| Sbjct 113 SEFERHAEGTYTSDITSYLEGQAAKEFIAW#LVNGRGRRDFPEKALMAEEMGRRHADGTFT 172 Query 105 SDV 107 \|\|+ Sbjct 173 SDI 175 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\| +\| \|\| \|++\|++\|#\|+\|\|\| + ++ Sbjct 164 RRHADGTFTSDINKILDDMAAKEFLKW#LINTKVTQRDL 201
Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide 1 (GLP-1); Glucagon-like peptide 1(7-37) (GLP-1(7-37)); Glucagon-like peptide 1(7-36) (GLP-1(7-36)); Glucagon-like peptide 2 (GLP-2)	Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKR------------------NRNNIA 89 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\| + \| +\| Sbjct 48 NEVKRHSQGTFTSDYSKYLDTRRAQDFVQW#LMNTKRSGQQGVEEREKENLLDQLSSNGLA 107 Query 90 KRHDEFERHAEGTFTSDV 107 + \| \|+\|\|\|\|+\| +\|\|\|+ Sbjct 108 RHHAEYERHADGRYTSDI 125 Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAK---RHDEFERHAEGTFTS 105 \| +\|\|+ \| +\|\|\| \| \|\|+ + \|++\|+ \|#\|+\| + \|+ + + \| \|\|\|+\|\|\|\|\| Sbjct 112 EYERHADGRYTSDISSYLEGQAAKEFIAW#LVNGRGRRDFLEEAGTADDIGRRHADGTFTS 171 Query 106 D 106 \| Query 106 D 106 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\|\|++ \|\| \|+\|++\|#\|+\| \| + ++ Sbjct 162 RRHADGTFTSDYNQLLDDIATQEFLKW#LINQKVTQRDL 199
Capra hircus	Query 86 NNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 86 NNIAKRHDEFERHAEGTFTSDV 107 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNR 85 +\| +\|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|+ \|#\|+ + \| Sbjct 8 DEFERHAEGTFTSDVSSYLEGQAAKEFIAW#LVKGRGRR 45
Oncorhynchus tshawytscha	Query 54 SQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \| \|\|\|++\|\|\|\|\| + \| \|\|\|\|\|\|\|#\|\|\|+\|\|+ +\|\|\|+\|\|+\|\|\|\| Sbjct 2 SXGTFSNDYSKYQEERMAQDFVQW#LMNSKRS-------GAPSKRHADGTYTSDV 48 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNR 85 \|\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|\| \|#\| + + \| Sbjct 37 KRHADGTYTSDVSTYLQDQAAKDFVSW#LKSGRARR 71
Ictalurus punctatus	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 +\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|#\|\|\|+\|\|+ \|\|\|+\|\|+\|\|\|\| Sbjct 49 RRHSEGTFSNDYSKYLETRRAQDFVQW#LMNSKRS-------DSPARRHADGTYTSDV 98 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEF--ERHAEGTFTSDV 107 +\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|+ \|#\| + + + + \| + \|\| +\|+\|\|\|\|\| Sbjct 87 RRHADGTYTSDVSSYLQDQAAKDFITW#LKSGQPKPDAVETRSVDTLRRRHVDGSFTSDV 145 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRH 92 +\|\| \|+\|\|\|\| +\| \|\|\| \|++++\|\|#+\|\|+ + + \|\|\| Sbjct 134 RRHVDGSFTSDVNKVLDSLAAKEYLQW#VMNSPSSAS--GKRH 173
Rattus norvegicus	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHA 99 \|+++\|\|\| \|\|\|\| +\| \| \|\|\|\| \|#\|+ \| +\|+ +\|+ +\| \| Sbjct 43 RYAEGTFISDYSIAMDKIRQQDFVNW#LLAQKGKKND--WKHNLTQREA 88
Canis familiaris	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHA 99 \|+++\|\|\| \|\|\|\| +\| \| \|\|\|\| \|#\|+ \| +\|+ +\|+ +\| \| Sbjct 43 RYAEGTFISDYSIAMDKIRQQDFVNW#LLAQKGKKND--WKHNITQREA 88
Ambloplites rupestris	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSD 106 \|\|\|\|\|\|\|+\|\|+ \|\|+ \|+\|\|\|\|++\|#\|\|\| \| \|+ \|++ \|\|\|+\|\|\|\| \| Sbjct 1 HSQGTFTNDYTNYLEDRQAQDFIRW#LMNNK--RSGAAEK-----RHADGTFTDD 47 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDE--FERHAEGTFTSDV 107 +\|\|\|+ \|\|\|\| \| \| + +\|\|\| #\| + + \| \| \| \|\| \|\|+\|\|\|\|+ Sbjct 36 EKRHADGTFTDDASSDFYDQAIKDFVAK#LKSGQDRREPETDRRREAFSRRHVEGSFTSDI 95
Pan troglodytes	Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| +++\| \|\|\|\|\|\|\|\|\|\|\| Sbjct 970 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRN-SSVA------YRHAEGTFTSDV 1022 Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNR 85 \|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|+ \|#\|+ + \| Sbjct 1012 RHAEGTFTSDVSSYLEGQAAKEFIAW#LVKGRGRR 1045
Xenopus tropicalis	Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|#\|\|\|\|\|\|+ +++\|+ ++\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 49 EVKRHSQGTFTSDYSKYLDSRRAQDFIQW#LMNTKRS-GGLSRRNADYERHAEGTFTSDV 106 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 +\|\|++\|\|+\|+\| ++\|\|+ + \|++\|++\|#\|+\| \| + + \|\|\|\|\|\|\|\|+\|+ Sbjct 140 ERHAEGTYTNDVTEYLEEKAAKEFIEW#LINGK-------PKKIRYSRHAEGTFTNDM 189 Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFE---RHAEGTFTSDV 107 \|\|++\|\|\|\|+\| + \|\|+ + \|++\|\| \|#\|+ \| + \| ++ \| \| \|\|\|+\|+\|\|+\|+ Sbjct 179 RHAEGTFTNDMTNYLEEKAAKEFVGW#LIK-GRPKRNFSEAHSVEEMDRRHADGSFTNDI 236 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTK 82 \|+\|\|+ \|+\|\|+\| +\| \|\| \|\|+\|+ \|#++\|\|+ Sbjct 224 DRRHADGSFTNDINKVLDIIAAQEFLDW#VINTQ 256
Danio rerio	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|#\|\|\| \|\|\| ++ +\|\|\|\|\|\|+\|\|\|\| Sbjct 48 KRHSEGTFSNDYSKYLETRRAQDFVQW#LMNAKRNGGSV-------KRHAEGTYTSDV 97 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#L 78 \|\|\|++\|\|+\|\|\| \| \|\| + \|\| \|\| \|#\| Sbjct 86 KRHAEGTYTSDVSSYLQDQAAQSFVAW#L 113
Bos taurus	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSD 106 \|\|+ \| \|\|\|\|\|\|+ \| \|+ +++ #\|+ \|\| \|+\|++ +\|\|++ \|\| + Sbjct 80 RHADGVFTSDYSRLLGQLSAKKYLES#LIG-KRVSNSISEDQGPIKRHSDAVFTDN 133
Macaca mulatta	Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSD 106 + + \|\|+ \| \|\|\|\|+\|\| \| \|+ +++ #\|\| \|\| +\|\|++ +\|\|++ \|\| + Sbjct 76 SRNARHADGVFTSDFSKLLGQLSAKKYLES#LMG-KRVSSNISEDPVPVKRHSDAVFTDN 133
Heloderma suspectum	Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKR------------------NRNNIA 89 \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\| + \| +\| Sbjct 48 NEVKRHSQGTFTSDYSKYLDTRRAQDFVQW#LMNTKRSGQQGVEEREKENLLDQLSSNGLA 107 Query 90 KRHDEFERHAEGTFTSDV 107 + \| \|+\|\|\|\|+\| +\|\|\|+ Sbjct 108 RHHAEYERHADGRYTSDI 125 Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNR 85 \| +\|\|+ \| +\|\|\| \| \|\|+ + \|++\|+ \|#\|+\| + \| Sbjct 112 EYERHADGRYTSDISSYLEGQAAKEFIAW#LVNGRGRR 148
Squalus acanthias	Query 67 DSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|+\|\|\|+\|\|\|\|\|#\|\|+\|\|\|\| \|+ +\|\|\|\|\|\|+\|\|\|\| Sbjct 1 DNRRAKDFVQW#LMSTKRN-------GDKTKRHAEGTYTSDV 34 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#L--MNTKRNRNNIAKRHDEFERHAEGTF 103 ++ \|\|\|++\|\|+\|\|\| \| +\|+ \|\| #\| \| ++\| +\|+\|\|\| \| \|\| \|\|++ Sbjct 20 DKTKRHAEGTYTSDVDSLSDYFKAKRFVDS#LTSYNKRQNGRSISKRHVESTRHTEGSY 77 Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRN---------NIAKRHDEFERHA 99 \| \|\|++\|++ \| \| \|\|+++ \|+\|\|+ \|#\|+ + \| \| + \|\|\| Sbjct 68 ESTRHTEGSYR-DISSYLEAKAAKDFINW#LIKGRGRREFPEESKEIVNEVIPEEMDRRHA 126 Query 100 EGTFTSDV 107 +\|\|\|\|\|++ Sbjct 127 DGTFTSEI 134 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFE 96 \|+\|\|+ \|\|\|\|\|+ + \|\|+ \|++\|+ \|#++\|+\| \| \| \| \| Sbjct 122 DRRHADGTFTSEINIVLDTIAAKEFLNW#ILNSK----TIQSRDSEIE 164
Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 32 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRN 84 \|+ \|\|\|\|+\| + \|\|\|++ \|+\|\|\| \|#\| + ++ Sbjct 1 HADGTFTNDMTSYLDAKAARDFVSW#LARSDKS 32
Contains: Glicentin-related polypeptide (GRPP); Glucagon-2 (Glucagon II); Glucagon-like peptide 2 (Glucagon-like peptide II)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|#\| \|+\|\|\| \| \|\|\|+\|\|+\|\|\|\| Sbjct 50 KRHSEGTFSNDYSKYLETRRAQDFVQW#LKNSKRN--------GLFRRHADGTYTSDV 98 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#L 78 +\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|\| \|#\| Sbjct 87 RRHADGTYTSDVSSYLQDQAAKDFVSW#L 114
Sebastes caurinus	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|+\|\|\|++\|\|\|+\|\|+ \|+\|\|\|\|\|+\|#\|\|\| \|\|+ \|\| +\|\|\|+\|\|\|\|\|\|\| Sbjct 48 KRHSEGTFSNDYSRYLEERKAQDFVRW#LMNNKRS------GADE-KRHADGTFTSDV 97 Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDE--FERHAEGTFTSD 106 ++\|\|\|+ \|\|\|\|\|\| \| \|\| + +\|\|\| #\| + + \| + + \| \|\| +\|+\|\|\|\| Sbjct 84 DEKRHADGTFTSDVSSYLKDQAIKDFVNR#LKSGQVRRESETEWRGEAFSRRHVDGSFTSD 143 Query 107 V 107 \| Query 107 V 107 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDE 94 +\|\| \|+\|\|\|\| +\| \|\|\| \|++++ \|#+\| +\| + + ++ \|+ Sbjct 133 RRHVDGSFTSDVNKVLDSMAAKEYLLW#VMTSKPSGESKKRQEDQ 176
Tetraodon nigroviridis	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|#\| \|+\|\|\| + \| \|\|\|+\|\|+\|\|\|\| Sbjct 787 KRHSEGTFSNDYSKYLETRRAQDFVQW#LKNSKRNGS-------LFRRHADGTYTSDV 836 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNR 85 +\|\|+ \|\|+\|\|\| \| \|\| + \|++\|\| \|#\| + \| Sbjct 825 RRHADGTYTSDVSAYLQDQAAKEFVSW#LKTGRGRR 859
Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|\|+\|#\|\|\|+\| \| + +\|\|\|\|\|\|+\|\|\|+ Sbjct 48 KRHSEGTFSNDYSKYLETRRAQDFVEW#LMNSKSNGGSA-------KRHAEGTYTSDI 97 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#L 78 \|\|\|++\|\|+\|\|\| \| +\| + \|\|+\|\| \|#\| Sbjct 86 KRHAEGTYTSDISSFLRDQAAQNFVAW#L 113
Contains: Glicentin-related polypeptide 2 (GRPP 2); Glucagon-2; Glucagon-like peptide 1-2 (GLP 1-2); Glucagon-like peptide 2 (GLP 2)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\| \|+\|\|\|++ \|\|\|\| + \| \|+\|\|+ \|#\|\|\|+\|\|+ +\|\|\|+\|\|+\|\|\|\| Sbjct 50 KRQSEGTFSNYYSKYQEERMARDFLHW#LMNSKRS-------GAPSKRHADGTYTSDV 99 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHD--EFERHAEGTFTSDV 107 \|\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|\| \|#\| + \| + + + \|\| +\|+\|\|\|\|\| Sbjct 88 KRHADGTYTSDVSTYLQDQAAKDFVSW#LKSGPARRESAEESMNGPMSRRHVDGSFTSDV 146 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHD 93 +\|\| \|+\|\|\|\| +\| \|\|\| \|++++ \|#+\| +\| + + ++ \| Sbjct 135 RRHVDGSFTSDVNKVLDSLAAKEYLLW#VMTSKTSGKSNKRQED 177
Contains: Glicentin-related polypeptide 1 (GRPP 1); Glucagon-1; Glucagon-like peptide 1-1 (GLP 1-1); Glucagon-like peptide 2 (GLP 2)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|+\|\|\|++\|\|\|\|\| + \| \|\|\|\|\|\|\|#\|\|\|+\|\|+ +\|\|\|+\|\|+\|\|\|\| Sbjct 50 KRHSEGTFSNDYSKYQEERMAQDFVQW#LMNSKRS-------GAPSKRHADGTYTSDV 99 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNI--AKRHDEFERHAEGTFTSDV 107 \|\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|\| \|#\| + + \| + ++ \|\| +\|+\|\|\|\|\| Sbjct 88 KRHADGTYTSDVSTYLQDQAAKDFVSW#LKSGRARRESAEESRNGPMSRRHVDGSFTSDV 146 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHD 93 +\|\| \|+\|\|\|\| +\| \|\|\| \|++++ \|#+\| +\| + + ++ \| Sbjct 135 RRHVDGSFTSDVNKVLDSLAAKEYLLW#VMTSKTSGKSNKRQED 177
Heloderma horridum	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMN 80 \|\|\|\| \|\|\|\|\|\| \|\| ++ + \|++\|#\| \| Sbjct 46 KRHSDGTFTSDLSKQMEEEAVRLFIEW#LKN 75
Mus musculus	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHA 99 \|+++\|\|\| \|\|\|\| +\| \| \|\|\|\| \|#\|+ + +++ +\|+ +\| \| Sbjct 43 RYAEGTFISDYSIAMDKIRQQDFVNW#LLAQRGKKSD--WKHNITQREA 88
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 33 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNTK 82 \|+ \|+\|\|\|\| +\| \|\|+ \|++\|+ \|#\|++\|\| Sbjct 1 HADGSFTSDINKVLDTIAAKEFLNW#LISTK 30
Protopterus dolloi	Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \| \|\|\|\|\|\|\|\| \|\|\|+\| \|+++ \| \| \|\| #\|\|+\|\|\| ++\|\|\|+\| \|\|\|\|\|\|\|+\|\|\|+ Sbjct 28 EAKRHSQGTFMSDYAKLLEAKHALDLVQR#LMSTKRT-GAVSKRHNEIERHAEGTYTSDI 85 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFE-RHAEGTFTSD 106 \|\| +\|\|++\|\|+\|\|\| \| \|\|+ + +\|++\|#\|\| + \|+ +\| + \|\|\|+\|\| \|\|\| Sbjct 71 NEIERHAEGTYTSDISSYLEGQAVNEFIRW#LMKGRGRRDFSETDIEEMDRRHADGTVTSD 130 Query 107 V 107 + Sbjct 131 I 131 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTK 82 \|+\|\|+ \|\| \|\|\| + \|+\| ++\|+ \|#\|+\| \| Sbjct 119 DRRHADGTVTSDINSVLESIATREFLNW#LINAK 151
Contains: Intestinal peptide PHI-42; Intestinal peptide PHI-27 (Peptide histidine isoleucinamide 27); Vasoactive intestinal peptide (VIP) (Vasoactive intestinal polypeptide)	Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSD 106 + + \|\|+ \| \|\|\|\|\|\|+ \| \|+ +++ #\|+ \|\| ++\|++ +\|\|++ \|\| + Sbjct 76 SRNARHADGVFTSDYSRLLGQISAKKYLES#LIG-KRISSSISEDPVPIKRHSDAVFTDN 133
Agkistrodon piscivorus	Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRH---DEFERHAEGTFTS 105 \| +\|\|+ \|\|+\|\|\| \| \|\|+ + \|++\|+ \|#\|+\| + \|+ \| \| \|\|\|+\|\|\|\|\| Sbjct 32 EYERHADGTYTSDISSYLEGQAAKEFIAW#LVNGRGRRDFSEGAHTGEDLGRRHADGTFTS 91 Query 106 D 106 \| Query 106 D 106 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\|\|+\| \|\| \|+\|++\|#\|+\| \| + ++ Sbjct 82 RRHADGTFTSDYNKLLDDIATQEFLKW#LINQKVTQRDL 119 Query 66 LDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|+ \| ++\|+ #\| + \| +\|+ \| \|+\|\|\|\|+\|\|+\|\|\|+ Sbjct 9 LEDREKENFLDQ#LAS-----NGLARPHAEYERHADGTYTSDI 45
Contains: Glucagon-2 (Glucagon II) (Glu II); Glucagon-like peptide 2-II (GLP-2II)	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|\|+\|\|\|\|\|\|\|+\|\| ++\|+\|\|\| \|#\|+\|\|\|\| + + +\|\|++\|+\|\|+\|+ Sbjct 43 RHSQGSFTSDYSKHLDVKQAKDFVTW#LLNTKRRGVDAQAGANLEKRHSDGSFTNDM 98 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKR 83 \| +\|\|\|\| \|+\|\|+\| + \|\| \|++\|++\|#\| \| Sbjct 84 NLEKRHSDGSFTNDMNVMLDRMSAKNFLEW#LKQQGR 119
Arvicanthis ansorgei	Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSD 106 + + \|\|+ \| \|\|\|\|\|\|+ \| \|+ +++ #\|+ \|\| ++\| + +\|\|++ \|\| + Sbjct 59 SRNARHADGVFTSDYSRLLGQISAKKYLES#LIG-KRISSSILEDPVPIKRHSDAVFTDN 116
synthetic construct	Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRN---NIAKRHDEFERHAEGTFT 104 +\| +\|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|+ \|#\|+ + \|+ +\| + \|\|\|+\|+\|+ Sbjct 93 DEFERHAEGTFTSDVSSYLEGQAAKEFIAW#LVKGRGRRDFPEEVAIVEELGRRHADGSFS 152 Query 105 SDV 107 ++ Sbjct 153 DEM 155 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTK 82 +\|\|+ \|+\|+ + + \|\|+ \|+\|\|+ \|#\|+ \|\| Sbjct 144 RRHADGSFSDEMNTILDNLAARDFINW#LIQTK 175
Contains: Glicentin-related polypeptide (GRPP); Glucagon-1 (Glucagon I); Glucagon-like peptide 1 (Glucagon-like peptide I)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|+\|\|\|++\|\|\|\|\|\|+ \|+\|\|+\|\|+\|#\|\|\| \| \|+ +\|++ \|\|\|+\|\|\|\|\|\|\| Sbjct 51 KRHSEGTFSNDYSKYLEDRKAQEFVRW#LMNNK--RSGVAEK-----RHADGTFTSDV 100 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQW#L 78 +\|\|\|+ \|\|\|\|\|\| \| \|\| + +\|\|\| #\| Sbjct 88 EKRHADGTFTSDVSSYLKDQAIKDFVDR#L 116
Phodopus sungorus	Query 56 GTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRN---NIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|\|\| \| \|\|+ + \|++\|+ \|#\|+ + \|+ + + \|\|\|+\|+\|+ ++ Sbjct 1 GTFTSDVSSYLEGQAAKEFIAW#LVKGRGRRDFPEEVTIVEELGRRHADGSFSDEM 55 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTK 82 +\|\|+ \|+\|+ + + \|\|\| +\|\|+ \|#\|+ \|\| Sbjct 44 RRHADGSFSDEMNTILDSLATRDFINW#LIQTK 75
Neoceratodus forsteri	Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \| \|\|\|\|\|\|\|\| \|\|\|+\| \|++\| \| \|\|\|\| #\|\|\|\|\|\|\| ++\|\|\| \| \|\|\|\|\|\|\|+\|\|\|+ Sbjct 20 EAKRHSQGTFMSDYAKLLEARHALDFVQR#LMNTKRN-GGVSKRHSEIERHAEGTYTSDI 77 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEF-ERHAEGTFTSD 106 +\| +\|\|++\|\|+\|\|\| \| \|\|+ + \| +\|\|+\|#\|+ + \|+ +\| \|\|\|+\|\| \|+\| Sbjct 63 SEIERHAEGTYTSDISSYLEGQAANEFVRW#LLKGRGRRDFSDTDAEEMGRRHADGTITND 122 Query 107 V 107 + Sbjct 123 M 123 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTK 82 +\|\|+ \|\| \|+\| + \|+\| ++\|+ \|#\|+\|+\| Sbjct 112 RRHADGTITNDMNNVLESIATREFLNW#LINSK 143
Sus scrofa	Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 49 EDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNKNNIAKRHDEFERHAEGTFTSDV 107 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRN---NIAKRHDEFERHAEGTFT 104 +\| +\|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|+ \|#\|+ + \|+ + + \|\|\|+\|+\|+ Sbjct 93 DEFERHAEGTFTSDVSSYLEGQAAKEFIAW#LVKGRGRRDFPEEVTIVEELRRRHADGSFS 152 Query 105 SDV 107 ++ Sbjct 153 DEM 155 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTK 82 +\|\|+ \|+\|+ + + \|\|+ +\|\|+ \|#\|++\|\| Sbjct 144 RRHADGSFSDEMNTVLDNLATRDFINW#LLHTK 175
Canis lupus familiaris	Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNKNNIAKRHDEFERHAEGTFTSDV 107 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRN---NIAKRHDEFERHAEGTFT 104 +\| +\|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|+ \|#\|+ + \|+ +\| + \|\|\|+\|+\|+ Sbjct 93 DEFERHAEGTFTSDVSSYLEGQAAKEFIAW#LVKGRGRRDFPEEVAIVEEFRRRHADGSFS 152 Query 105 SDV 107 ++ Sbjct 153 DEM 155 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTK 82 +\|\|+ \|+\|+ + + \|\|+ +\|\|+ \|#\|+ \|\| Sbjct 144 RRHADGSFSDEMNTVLDTLATRDFINW#LLQTK 175
Contains: Glucagon; Glucagon-36 (Oxyntomodulin); Glucagon-like peptide	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|#\|\|+\|\|\|+ \| \|\|+\|\|+\|\|\|\| Sbjct 1 HSQGTFTNDYSKYLDTRRAQDFVQW#LMSTKRS-GGITXXXXXXXXHADGTYTSDV 54 Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNR 85 \|+ \|\|+\|\|\| \| \|\| + \|+ \|\| \|#\| + \| Sbjct 45 HADGTYTSDVSSYLQDQAAKKFVTW#LKQGQDRR 77
Hydrolagus colliei	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFERHAEGTFTSDV 107 \|\|\|+ \| \|+\|\|\|\|\|\|\|\|+\|\| +\|\|\|\|\|#\|++\|\|\|\| \| \|+ +\|\|\|+\| +\|\|\|\| Sbjct 30 KRHTDGIFSSDYSKYLDNRRTKDFVQW#LLSTKRNGANT----DKTKRHADGIYTSDV 82 Query 48 NEDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNN--IAKRHDEFERHAEGTFTS 105 ++ \|\|\|+ \| +\|\|\| + \| +++ \|\|+ #\| \| + +\|+ ++\|\|\| \| \| \| + + Sbjct 68 DKTKRHADGIYTSDVASLTDYLKSKRFVES#LSNYNKRQNDRRMSKRHVEGARRTEEDYPN 127 Query 106 D 106 \| Query 106 D 106 Query 49 EDKRHSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRNNIAKRHDEFE 96 \| \| ++ + +\|+\| \|\|+++ \|+\|\|+ \|#\|+ + \| + \|+ \| \| Sbjct 116 EGARRTEEDYPNDFSSYLEAKAAKDFIDW#LIK-GQGRRDFAEESREIE 162
Heloderma suspectum, venom, Peptide, 39 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMN 80 \| +\|\|\|\|\|\| \|\| ++ + \|++\|#\| \| Sbjct 1 HGEGTFTSDLSKQMEEEAVRLFIEW#LKN 28
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 33 aa	Query 56 GTFTSDYSKYLDSRRAQDFVQW#LMNTKRNRN 86 \|\|+\|\|\| \| \|\| + \|++\|\| \|#\| + \|+ Sbjct 3 GTYTSDVSSYLQDQAAKEFVSW#LKTGRGRRD 33
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 34 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQW#LMNTKRNR 85 \|+ \|\| \|\|\| \| +\|+ + ++\|+ \|#\|++ + \| Sbjct 1 HADGTLTSDISSFLEKQATKEFIAW#LVSGRGRR 33

[Site 4] FTSDYSKYLD⁶⁷-SRRAQDFVQW

Asp⁶⁷ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Phe⁵⁸	Thr⁵⁹	Ser⁶⁰	Asp⁶¹	Tyr⁶²	Ser⁶³	Lys⁶⁴	Tyr⁶⁵	Leu⁶⁶	Asp⁶⁷

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser⁶⁸	Arg⁶⁹	Arg⁷⁰	Ala⁷¹	Gln⁷²	Asp⁷³	Phe⁷⁴	Val⁷⁵	Gln⁷⁶	Trp⁷⁷

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
ADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFER

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	129.00	9	glucagon, isoform CRA_b
2	Pan troglodytes	106.00	2	PREDICTED: fibroblast activation protein, alpha su
3	N/A	79.70	43	0
4	Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 33 aa	64.70	1	AAB31092.1
5	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 29 aa	61.60	1	glucagon=proglucagon-derived 3.5 kda peptide
6	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 32 aa	60.80	1	glucagon II
7	Contains: Glucagon; Glucagon-like peptide	60.10	4	GLUC_AMICA Glucagon precursor
8	Amia calva=bowfin, pancreatic tissues, Peptide, 29 aa	60.10	1	glucagon
9	Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 29 aa	58.50	1	glucagon G-29
10	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 36 aa	57.80	1	GLUC1_ORENI Glucagon-1 (Glucagon I) gb
11	Lampetra fluviatilis=river lamprey, small intestine, Peptide, 29 aa	56.20	1	GLUC_LAMFL Glucagon gb
12	Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 29 aa	53.50	1	glucagon
13	deshis1, Desphe6, Glu9	53.50	1	A Chain A, Nmr Solution Structure Of The Glucagon
14	Contains: Glucagon-1 (Glucagon I); Glucagon-like peptide 1-I (GLP-1I); Glucagon-like peptide 2-I (GLP-2I)	47.00	1	GLUC1_PETMA Glucagon-1 precursor
15	Gallus gallus	44.30	4	glucagon isoform 1
16	Meleagris gallopavo	44.30	2	preproglucagon B
17	Agkistrodon piscivorus	44.30	1	proglucagon
18	Capra hircus	43.90	1	glucagon
19	Oncorhynchus tshawytscha	43.50	1	S78474_1 proglucagon
20	Monodelphis domestica	42.70	3	PREDICTED: similar to Gastric inhibitory polypepti
21	Xenopus laevis	42.40	5	proglucagon I
22	Rattus norvegicus	42.40	3	Glucose-dependent insulinotropic peptide
23	Xenopus tropicalis	42.40	2	glucagon
24	Canis familiaris	42.40	2	PREDICTED: similar to Gastric inhibitory polypepti
25	Danio rerio	42.00	4	hypothetical protein LOC494052
26	Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide 1 (GLP-1); Glucagon-like peptide 1(7-37) (GLP-1(7-37)); Glucagon-like peptide 1(7-36) (GLP-1(7-36)); Glucagon-like peptide 2 (GLP-2)	42.00	1	GLUC_HELSU Glucagon precursor
27	Heloderma suspectum	41.60	2	proglucagon
28	Hoplobatrachus rugulosus	41.60	1	AF324209_1 proglucagon
29	Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 32 aa	41.20	1	glucagon-like peptide, GLP
30	Contains: Glicentin-related polypeptide (GRPP); Glucagon-2 (Glucagon II); Glucagon-like peptide 2 (Glucagon-like peptide II)	41.20	1	GLUC2_LOPAM Glucagon-2 precursor (Glucagon II)
31	Heloderma horridum	40.80	1	EXE3_HELHO Exendin-3 precursor emb
32	Bos taurus	40.40	5	PREDICTED: similar to Gastric inhibitory polypepti
33	Ictalurus punctatus	40.40	3	proglucagon I
34	Macaca mulatta	40.40	3	PREDICTED: gastric inhibitory polypeptide
35	Protopterus dolloi	40.40	1	proglucagon
36	Sebastes caurinus	40.00	4	proglucagon II
37	Tetraodon nigroviridis	40.00	2	unnamed protein product
38	Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide	40.00	2	GLUC_CARAU Glucagon precursor
39	Contains: Glicentin-related polypeptide 2 (GRPP 2); Glucagon-2; Glucagon-like peptide 1-2 (GLP 1-2); Glucagon-like peptide 2 (GLP 2)	40.00	1	GLUC2_ONCMY Glucagon-2 precursor (Glucagon II)
40	Contains: Glicentin-related polypeptide 1 (GRPP 1); Glucagon-1; Glucagon-like peptide 1-1 (GLP 1-1); Glucagon-like peptide 2 (GLP 2)	40.00	1	GLUC1_ONCMY Glucagon-1 precursor (Glucagon I)
41	Neoceratodus forsteri	39.70	1	proglucagon
42	Mus musculus	39.30	5	gastric inhibitory polypeptide
43	Bufo marinus	38.90	1	proglucagon
44	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 33 aa	38.90	1	glucagon-like peptide-2, GLP-2=proglucagon-derived
45	Contains: Glucagon-2 (Glucagon II) (Glu II); Glucagon-like peptide 2-II (GLP-2II)	38.10	1	GLUC2_PETMA Glucagon-2 precursor
46	synthetic construct	37.00	1	Homo sapiens glucagon
47	Contains: Glicentin-related polypeptide (GRPP); Glucagon-1 (Glucagon I); Glucagon-like peptide 1 (Glucagon-like peptide I)	36.60	1	GLUC1_LOPAM Glucagon-1 precursor (Glucagon I)
48	Phodopus sungorus	36.60	1	glucagon precursor
49	Sus scrofa	36.20	1	glucagon
50	Canis lupus familiaris	35.80	1	glucagon
51	Contains: Glucagon; Glucagon-36 (Oxyntomodulin); Glucagon-like peptide	35.80	1	GLUC_LEPSP Glucagon precursor
52	Heloderma suspectum, venom, Peptide, 39 aa	35.40	1	A Chain A, Solution Structure Of Exendin-4 In 30-V
53	Hydrolagus colliei	35.40	1	proglucagon
54	Squalus acanthias	35.40	1	proglucagon
55	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 34 aa	34.70	1	glucagon-like peptide-1, GLP-1=proglucagon-derived
56	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 33 aa	34.70	1	GLUC2_ORENI Glucagon-2 (Glucagon II) gb
57	Candida glabrata	32.70	1	unnamed protein product
58	Ambloplites rupestris	32.30	1	proglucagon

Top-ranked sequences

organism	matching
Homo sapiens	Query 38 ADPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 38 ADPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97
Pan troglodytes	Query 38 ADPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDE 94 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| +++\| \|\| \| Sbjct 960 ADPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRN-SSVAYRHAE 1015
N/A	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIA 89 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 1 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNKNNIA 37
Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 33 aa	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRN 84 \|\|+\|\|\|\|\|\|\|\|\|\|+\|#+\|\|\|+\|\|\|\|\|\|\|+\|\|\|\| Sbjct 1 HSEGTFTSDYSKYMD#NRRAKDFVQWLMSTKRN 32
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNT 81 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#+\|\|\|\|\|\|+\|\|\|\|+\| Sbjct 1 HSQGTFTSDYSKYLD#NRRAQDFIQWLMST 29
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 32 aa	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRN 84 \|\|+\|\|\|++\|\|\|\|\|\|+#+\|\|\|\|\|\|\|\|\|\| \|+\|\|\| Sbjct 1 HSEGTFSNDYSKYLE#TRRAQDFVQWLKNSKRN 32
Contains: Glucagon; Glucagon-like peptide	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNT 81 \|\|\|\|\|\|\|+\|\|\|\|\|+\|#+\|\|\|\|\|\|\|\|\|\|\|+\| Sbjct 1 HSQGTFTNDYSKYMD#TRRAQDFVQWLMST 29
Amia calva=bowfin, pancreatic tissues, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNT 81 \|\|\|\|\|\|\|+\|\|\|\|\|+\|#+\|\|\|\|\|\|\|\|\|\|\|+\| Sbjct 1 HSQGTFTNDYSKYMD#TRRAQDFVQWLMST 29
Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNT 81 \|\|+\|\|\|\|\|\|\|\|\|\|+\|#+\|\|\|+\|\|\|\|\|\|\|+\| Sbjct 1 HSEGTFTSDYSKYMD#NRRAKDFVQWLMST 29
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 36 aa	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRN 84 \|\|+\|\|\|++\|\|\|\|\|\|+# \|+\|\|\|\|\|+\|\|\|\| \|\|+ Sbjct 1 HSEGTFSNDYSKYLE#DRKAQDFVRWLMNNKRS 32
Lampetra fluviatilis=river lamprey, small intestine, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNT 81 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|#\|++\|+\|\|\| \|\|\|\|\| Sbjct 1 HSQGSFTSDYSKYLD#SKQAKDFVIWLMNT 29
Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMN 80 \|\|+\|\|\|\|\|\|\|\|\|\|\|+#+++\|+\|\|\|+\|\|\|\| Sbjct 1 HSEGTFTSDYSKYLE#NKQAKDFVRWLMN 28
deshis1, Desphe6, Glu9	Query 54 SQGTFTSDYSKYLD#SRRAQDFVQWLMNT 81 \|\|\|\| \|\|+\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 SQGT-TSEYSKYLD#SRRAQDFVQWLMNT 27
Contains: Glucagon-1 (Glucagon I); Glucagon-like peptide 1-I (GLP-1I); Glucagon-like peptide 2-I (GLP-2I)	Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRH 92 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|+#+++\|+\|\|\|+\|\|\|\| \|\| + + +\|\| Sbjct 41 KRHSEGTFTSDYSKYLE#NKQAKDFVRWLMNAKRGGSELQRRH 82 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNR---------NNIAKRHDE 94 +\|\|+ \|\|\|\|+\| + \|\|\|#++ \|+\|\|\| \|\| + ++\| \|+ \|\|\| \| Sbjct 80 RRHADGTFTNDMTSYLD#AKAARDFVSWLARSDKSRRDGGDHLAENSEDKRHAE 132
Gallus gallus	Query 38 ADPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRN------------- 84 ++\|\| + \|+\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 40 SEPLDESRQLNEVKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMSTKRNGQQGQEDKENDKF 99 Query 85 -----RNNIAKRHDEFER 97 \| \|+\|\|\| \|\|\|\| Sbjct 100 PDQLSSNAISKRHSEFER 117 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\| +\| \|\|# \|++\|++\|\|+\|\|\| + ++ Sbjct 164 RRHADGTFTSDINKILD#DMAAKEFLKWLINTKVTQRDL 201 Query 48 NEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRN 86 +\| +\|\|++\|\|+\|\|\| + \|\|+# + \|++\|+ \|\|+\| + \|+ Sbjct 113 SEFERHAEGTYTSDITSYLE#GQAAKEFIAWLVNGRGRRD 151
Meleagris gallopavo	Query 38 ADPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRN------------- 84 ++\|\| + \|+\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| Sbjct 40 SEPLDESRQLNEVKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMSTKRNGQQGQEDKENDEF 99 Query 85 -----RNNIAKRHDEFER 97 \| \|+\|\|\| \|\|\|\| Sbjct 100 PDQLSSNAISKRHSEFER 117 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\| +\| \|\|# \|++\|++\|\|+\|\|\| + ++ Sbjct 164 RRHADGTFTSDINKILD#DMAAKEFLKWLINTKVTQRDL 201 Query 48 NEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRN 86 +\| +\|\|++\|\|+\|\|\| + \|\|+# + \|++\|+ \|\|+\| + \|+ Sbjct 113 SEFERHAEGTYTSDITSYLE#GQAAKEFIAWLVNGRGRRD 151
Agkistrodon piscivorus	Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\|\|+\| \|\|# \|+\|++\|\|+\| \| + ++ Sbjct 82 RRHADGTFTSDYNKLLD#DIATQEFLKWLINQKVTQRDL 119 Query 49 EDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRN 86 \| +\|\|+ \|\|+\|\|\| \| \|\|+# + \|++\|+ \|\|+\| + \|+ Sbjct 32 EYERHADGTYTSDISSYLE#GQAAKEFIAWLVNGRGRRD 69
Capra hircus	Query 48 NEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNR 85 +\| +\|\|++\|\|\|\|\|\| \| \|\|+# + \|++\|+ \|\|+ + \| Sbjct 8 DEFERHAEGTFTSDVSSYLE#GQAAKEFIAWLVKGRGRR 45
Oncorhynchus tshawytscha	Query 54 SQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRH 92 \| \|\|\|++\|\|\|\|\| +# \| \|\|\|\|\|\|\|\|\|\|+\|\|+ +\|\|\| Sbjct 2 SXGTFSNDYSKYQE#ERMAQDFVQWLMNSKRS-GAPSKRH 39 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNR 85 \|\|\|+ \|\|+\|\|\| \| \|\| # + \|+\|\|\| \|\| + + \| Sbjct 37 KRHADGTYTSDVSTYLQ#DQAAKDFVSWLKSGRARR 71
Monodelphis domestica	Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 +\|+++\|\|\| \|\|\|\| +\|# \|\|\|\| \|\|++ \| +\| + \|\|+ \|\| Sbjct 51 QRYAEGTFISDYSITMD#KIMQQDFVNWLLSQKGKKN--SWRHNITER 95
Xenopus laevis	Query 39 DPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 + + \| +\|+ \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+ +++\|+ ++\|\| Sbjct 39 EAVDDSEQLKEVKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRS-GELSRRNADYER 96 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 +\|\|++\|\|\|\|\|\| ++ \|\|# + \|++\|+ \|\|+\| ++ \|++\|+ \|\|\|\| Sbjct 95 ERHAEGTFTSDVTQQLD#EKAAKEFIDWLINGGPSKEIISRRNAEFER 141 Query 50 DKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNI 88 \|+\|\|+ \|+\|\|+\| +\| \|\|# \|\|+\|+ \|++\|\|+ ++ Sbjct 224 DRRHADGSFTNDINKVLD#IIAAQEFLDWVINTQETERDL 262 Query 52 RHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRN 86 \|\|++\|\|\|\|+\| + \|\|+# + \|++\|\| \|\|+ + \|\| Sbjct 179 RHAEGTFTNDMTNYLE#EKAAKEFVGWLIKGRPKRN 213 Query 49 EDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDE 94 \| +\|\|++\|\|+\|+\| ++\|\|+# + \|++\|++\|\|+ \| + + \|\| \| Sbjct 138 EFERHAEGTYTNDVTEYLE#EKAAKEFIEWLIKGKPKKIRYS-RHAE 182
Rattus norvegicus	Query 52 RHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNN 87 \|+++\|\|\| \|\|\|\| +\|# \| \|\|\|\| \|\|+ \| +\|+ Sbjct 43 RYAEGTFISDYSIAMD#KIRQQDFVNWLLAQKGKKND 78
Xenopus tropicalis	Query 39 DPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 + + \| \|+ \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|+ +++\|+ ++\|\| Sbjct 39 EAVDDSQQLKEVKRHSQGTFTSDYSKYLD#SRRAQDFIQWLMNTKRS-GGLSRRNADYER 96 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 +\|\|++\|\|\|\|\|\| +++\|\|# + \|++\|+ \|\|+\| ++ \|++\|+ + \|\| Sbjct 95 ERHAEGTFTSDVTQHLD#EKAAKEFIDWLINGGPSKEIISRRNADVER 141 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDE 94 +\|\|++\|\|+\|+\| ++\|\|+# + \|++\|++\|\|+\| \| + + \|\| \| Sbjct 140 ERHAEGTYTNDVTEYLE#EKAAKEFIEWLINGKPKKIRYS-RHAE 182 Query 52 RHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRN 86 \|\|++\|\|\|\|+\| + \|\|+# + \|++\|\| \|\|+ + \|\| Sbjct 179 RHAEGTFTNDMTNYLE#EKAAKEFVGWLIKGRPKRN 213 Query 50 DKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTK 82 \|+\|\|+ \|+\|\|+\| +\| \|\|# \|\|+\|+ \|++\|\|+ Sbjct 224 DRRHADGSFTNDINKVLD#IIAAQEFLDWVINTQ 256
Canis familiaris	Query 52 RHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNN 87 \|+++\|\|\| \|\|\|\| +\|# \| \|\|\|\| \|\|+ \| +\|+ Sbjct 43 RYAEGTFISDYSIAMD#KIRQQDFVNWLLAQKGKKND 78
Danio rerio	Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDE 94 \|\|\|\|+\|\|\|++\|\|\|\|\|\|+#+\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| ++ \|\|\| \| Sbjct 48 KRHSEGTFSNDYSKYLE#TRRAQDFVQWLMNAKRNGGSV-KRHAE 90 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWL 78 \|\|\|++\|\|+\|\|\| \| \|\| # + \|\| \|\| \|\| Sbjct 86 KRHAEGTYTSDVSSYLQ#DQAAQSFVAWL 113
Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide 1 (GLP-1); Glucagon-like peptide 1(7-37) (GLP-1(7-37)); Glucagon-like peptide 1(7-36) (GLP-1(7-36)); Glucagon-like peptide 2 (GLP-2)	Query 38 ADPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKR-------------- 83 \|+\|\| \| \|+\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 38 AEPLDDSRQLNEVKRHSQGTFTSDYSKYLD#TRRAQDFVQWLMNTKRSGQQGVEEREKENL 97 Query 84 ----NRNNIAKRHDEFER 97 + \| +\|+ \| \|+\|\| Sbjct 98 LDQLSSNGLARHHAEYER 115 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNI 88 +\|\|+ \|\|\|\|\|\|\|++ \|\|# \|+\|++\|\|+\| \| + ++ Sbjct 162 RRHADGTFTSDYNQLLD#DIATQEFLKWLINQKVTQRDL 199 Query 49 EDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRN 86 \| +\|\|+ \| +\|\|\| \| \|\|+# + \|++\|+ \|\|+\| + \|+ Sbjct 112 EYERHADGRYTSDISSYLE#GQAAKEFIAWLVNGRGRRD 149
Heloderma suspectum	Query 38 ADPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKR-------------- 83 \|+\|\| \| \|+\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 38 AEPLDDSRQLNEVKRHSQGTFTSDYSKYLD#TRRAQDFVQWLMNTKRSGQQGVEEREKENL 97 Query 84 ----NRNNIAKRHDEFER 97 + \| +\|+ \| \|+\|\| Sbjct 98 LDQLSSNGLARHHAEYER 115 Query 49 EDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNR 85 \| +\|\|+ \| +\|\|\| \| \|\|+# + \|++\|+ \|\|+\| + \| Sbjct 112 EYERHADGRYTSDISSYLE#GQAAKEFIAWLVNGRGRR 148
Hoplobatrachus rugulosus	Query 39 DPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 + \| \| \| \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|+ \|+\|\|+ +\|\|\| Sbjct 39 EALDDSDHHNEVKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNSKRS-GGISKRNVQFER 96 Query 50 DKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNT 81 \|+\|\|+ \|+\|\|\|\|++\| \|\|# + \|\|+\|+ \|++\|\| Sbjct 178 DRRHADGSFTSDFNKALD#IKAAQEFLDWIINT 209 Query 52 RHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRN 86 \|\|++\|\|\|\|\|\| + \|\|+# + \|++\|\| \|\|+ + \|\| Sbjct 134 RHAEGTFTSDMTSYLE#EKAAKEFVDWLIKGRPKRN 168 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDE 94 +\|\|++\|\|+\|+\| +++\|+# + \|++\|+ \|\|+ \| + ++ \|\| \| Sbjct 95 ERHAEGTYTNDVTQFLE#EKAAKEFIDWLIKGKPKKQRLS-RHAE 137
Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 32 aa	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRN 84 \|+ \|\|\|\|+\| + \|\|\|#++ \|+\|\|\| \|\| + ++ Sbjct 1 HADGTFTNDMTSYLD#AKAARDFVSWLARSDKS 32
Contains: Glicentin-related polypeptide (GRPP); Glucagon-2 (Glucagon II); Glucagon-like peptide 2 (Glucagon-like peptide II)	Query 41 LSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRH 92 \|++\| + \|\|\|\|+\|\|\|++\|\|\|\|\|\|+#+\|\|\|\|\|\|\|\|\|\| \|+\| \|\| + +\|\| Sbjct 40 LTEPIEPLNMKRHSEGTFSNDYSKYLE#TRRAQDFVQWLKNSK--RNGLFRRH 89 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWL 78 +\|\|+ \|\|+\|\|\| \| \|\| # + \|+\|\|\| \|\| Sbjct 87 RRHADGTYTSDVSSYLQ#DQAAKDFVSWL 114
Heloderma horridum	Query 39 DPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMN 80 \| \| ++ \|\|\|\| \|\|\|\|\|\| \|\| ++# + \|++\|\| \| Sbjct 34 DSASSESFASKIKRHSDGTFTSDLSKQME#EEAVRLFIEWLKN 75
Bos taurus	Query 52 RHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNN 87 \|+++\|\|\| \|\|\|\| +\|# \| \|\|\|\| \|\|+ \| +++ Sbjct 81 RYAEGTFISDYSIAMD#KIRQQDFVNWLLAQKGKKSD 116
Ictalurus punctatus	Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRH 92 +\|\|\|+\|\|\|++\|\|\|\|\|\|+#+\|\|\|\|\|\|\|\|\|\|\|\|+\|\|+ ++ \|+\|\| Sbjct 49 RRHSEGTFSNDYSKYLE#TRRAQDFVQWLMNSKRS-DSPARRH 89 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRH 92 +\|\| \|+\|\|\|\| +\| \|\|#\| \|++++\|\|+\|\|+ + + \|\|\| Sbjct 134 RRHVDGSFTSDVNKVLD#SLAAKEYLQWVMNSPSSAS--GKRH 173 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWL 78 +\|\|+ \|\|+\|\|\| \| \|\| # + \|+\|\|+ \|\| Sbjct 87 RRHADGTYTSDVSSYLQ#DQAAKDFITWL 114
Macaca mulatta	Query 52 RHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNN 87 \|+++\|\|\| \|\|\|\| +\|# \|\|\|\| \|\|+ \| +\|+ Sbjct 51 RYAEGTFISDYSIAMD#KIHQQDFVNWLLAQKGKKND 86
Protopterus dolloi	Query 39 DPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 + + + + \| \|\|\|\|\|\|\|\| \|\|\|+\| \|+#++ \| \| \|\| \|\|+\|\|\| ++\|\|\|+\| \|\| Sbjct 18 EAVDESREHTEAKRHSQGTFMSDYAKLLE#AKHALDLVQRLMSTKRT-GAVSKRHNEIER 75 Query 48 NEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 \|\| +\|\|++\|\|+\|\|\| \| \|\|+# + +\|++\|\|\| + \|+ +\| +\| Sbjct 71 NEIERHAEGTYTSDISSYLE#GQAVNEFIRWLMKGRGRRDFSETDIEEMDR 120 Query 42 SDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTK 82 \|+ \| \|+\|\|+ \|\| \|\|\| + \|+#\| ++\|+ \|\|+\| \| Sbjct 111 SETDIEEMDRRHADGTVTSDINSVLE#SIATREFLNWLINAK 151
Sebastes caurinus	Query 41 LSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRH 92 \|++\| ++ \|\|\|\|+\|\|\|++\|\|\|\|\|\|+#+\|\|\|\|\|\|\|\|\|\| \|+\|\|\| ++ +\|\| Sbjct 40 LAEPIELPNMKRHSEGTFSNDYSKYLE#TRRAQDFVQWLKNSKRN-GSLFRRH 90 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNR 85 +\|\|+ \|\|+\|\|\| \| \|\| # + \|++\|\| \|\| + \| Sbjct 88 RRHADGTYTSDVSSYLQ#DQAAKEFVYWLKTGRGRR 122
Tetraodon nigroviridis	Query 41 LSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRH 92 \|++\| ++ \|\|\|\|+\|\|\|++\|\|\|\|\|\|+#+\|\|\|\|\|\|\|\|\|\| \|+\|\|\| ++ +\|\| Sbjct 777 LTEPSDLSNMKRHSEGTFSNDYSKYLE#TRRAQDFVQWLKNSKRN-GSLFRRH 827 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNR 85 +\|\|+ \|\|+\|\|\| \| \|\| # + \|++\|\| \|\| + \| Sbjct 825 RRHADGTYTSDVSAYLQ#DQAAKEFVSWLKTGRGRR 859
Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide	Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDE 94 \|\|\|\|+\|\|\|++\|\|\|\|\|\|+#+\|\|\|\|\|\|\|+\|\|\|\|+\| \| + \|\|\|\| \| Sbjct 48 KRHSEGTFSNDYSKYLE#TRRAQDFVEWLMNSKSNGGS-AKRHAE 90 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWL 78 \|\|\|++\|\|+\|\|\| \| +\| # + \|\|+\|\| \|\| Sbjct 86 KRHAEGTYTSDISSFLR#DQAAQNFVAWL 113
Contains: Glicentin-related polypeptide 2 (GRPP 2); Glucagon-2; Glucagon-like peptide 1-2 (GLP 1-2); Glucagon-like peptide 2 (GLP 2)	Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRH 92 \|\| \|+\|\|\|++ \|\|\|\| +# \| \|+\|\|+ \|\|\|\|+\|\|+ +\|\|\| Sbjct 50 KRQSEGTFSNYYSKYQE#ERMARDFLHWLMNSKRS-GAPSKRH 90 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNN 87 \|\|\|+ \|\|+\|\|\| \| \|\| # + \|+\|\|\| \|\| + \| + Sbjct 88 KRHADGTYTSDVSTYLQ#DQAAKDFVSWLKSGPARRES 124 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHD 93 +\|\| \|+\|\|\|\| +\| \|\|#\| \|++++ \|+\| +\| + + ++ \| Sbjct 135 RRHVDGSFTSDVNKVLD#SLAAKEYLLWVMTSKTSGKSNKRQED 177
Contains: Glicentin-related polypeptide 1 (GRPP 1); Glucagon-1; Glucagon-like peptide 1-1 (GLP 1-1); Glucagon-like peptide 2 (GLP 2)	Query 39 DPL-SDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRH 92 \|\|\| \| ++ \|\|\|\|+\|\|\|++\|\|\|\|\| +# \| \|\|\|\|\|\|\|\|\|\|+\|\|+ +\|\|\| Sbjct 37 DPLLEDLMGVSNVKRHSEGTFSNDYSKYQE#ERMAQDFVQWLMNSKRS-GAPSKRH 90 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNN 87 \|\|\|+ \|\|+\|\|\| \| \|\| # + \|+\|\|\| \|\| + + \| + Sbjct 88 KRHADGTYTSDVSTYLQ#DQAAKDFVSWLKSGRARRES 124 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHD 93 +\|\| \|+\|\|\|\| +\| \|\|#\| \|++++ \|+\| +\| + + ++ \| Sbjct 135 RRHVDGSFTSDVNKVLD#SLAAKEYLLWVMTSKTSGKSNKRQED 177
Neoceratodus forsteri	Query 39 DPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 +\|+ + \| \|\|\|\|\|\|\|\| \|\|\|+\| \|+#+\| \| \|\|\|\| \|\|\|\|\|\|\| ++\|\|\| \| \|\| Sbjct 10 EPVDASREHTEAKRHSQGTFMSDYAKLLE#ARHALDFVQRLMNTKRN-GGVSKRHSEIER 67 Query 48 NEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRN 86 +\| +\|\|++\|\|+\|\|\| \| \|\|+# + \| +\|\|+\|\|+ + \|+ Sbjct 63 SEIERHAEGTYTSDISSYLE#GQAANEFVRWLLKGRGRRD 101 Query 42 SDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTK 82 \|\| \| +\|\|+ \|\| \|+\| + \|+#\| ++\|+ \|\|+\|+\| Sbjct 103 SDTDAEEMGRRHADGTITNDMNNVLE#SIATREFLNWLINSK 143
Mus musculus	Query 52 RHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNN 87 \|+++\|\|\| \|\|\|\| +\|# \| \|\|\|\| \|\|+ + +++ Sbjct 43 RYAEGTFISDYSIAMD#KIRQQDFVNWLLAQRGKKSD 78
Bufo marinus	Query 50 DKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNT 81 \|+\|\|+ \|+\|\|\|\|++\| \|\|# + \|\|+\|+ \|++\|\| Sbjct 107 DRRHADGSFTSDFNKALD#IKAAQEFLDWIINT 138 Query 52 RHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRN 86 \|\|++\|\|\|\|\|\| + +\|+# + \|++\|\| \|\|+ + \|\| Sbjct 62 RHAEGTFTSDMTSFLE#EKAAKEFVDWLIKGRPKRN 96 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDE 94 +\|\|++\|\|+\|+\| +++\|+# + \|++\|+ \|\|+ + ++ \|\| \| Sbjct 23 ERHAEGTYTNDVTQFLE#EKAAKEFIDWLLKGIPKKQRLS-RHAE 65
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 33 aa	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNTK 82 \|+ \|+\|\|\|\| +\| \|\|#+ \|++\|+ \|\|++\|\| Sbjct 1 HADGSFTSDINKVLD#TIAAKEFLNWLISTK 30
Contains: Glucagon-2 (Glucagon II) (Glu II); Glucagon-like peptide 2-II (GLP-2II)	Query 52 RHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRN------RNNIAKRHDE 94 \|\|\|\|\|+\|\|\|\|\|\|\|+\|\|# ++\|+\|\|\| \|\|+\|\|\|\| \|+ \|\|\| + Sbjct 43 RHSQGSFTSDYSKHLD#VKQAKDFVTWLLNTKRRGVDAQAGANLEKRHSD 91 Query 48 NEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKR 83 \| +\|\|\|\| \|+\|\|+\| + \|\|# \|++\|++\|\| \| Sbjct 84 NLEKRHSDGSFTNDMNVMLD#RMSAKNFLEWLKQQGR 119
synthetic construct	Query 38 ADPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 38 ADPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTK 82 +\|\|+ \|+\|+ + + \|\|#+ \|+\|\|+ \|\|+ \|\| Sbjct 144 RRHADGSFSDEMNTILD#NLAARDFINWLIQTK 175
Contains: Glicentin-related polypeptide (GRPP); Glucagon-1 (Glucagon I); Glucagon-like peptide 1 (Glucagon-like peptide I)	Query 43 DPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRH 92 +\| +++ \|\|\|\|+\|\|\|++\|\|\|\|\|\|+# \|+\|\|+\|\|+\|\|\|\| \|\|+ \|\|\| Sbjct 43 EPRELSNMKRHSEGTFSNDYSKYLE#DRKAQEFVRWLMNNKRS-GVAEKRH 91 Query 50 DKRHSQGTFTSDYSKYLD#SRRAQDFVQWL 78 +\|\|\|+ \|\|\|\|\|\| \| \|\| # + +\|\|\| \| Sbjct 88 EKRHADGTFTSDVSSYLK#DQAIKDFVDRL 116
Phodopus sungorus	Query 56 GTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRN 86 \|\|\|\|\|\| \| \|\|+# + \|++\|+ \|\|+ + \|+ Sbjct 1 GTFTSDVSSYLE#GQAAKEFIAWLVKGRGRRD 31 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTK 82 +\|\|+ \|+\|+ + + \|\|#\| +\|\|+ \|\|+ \|\| Sbjct 44 RRHADGSFSDEMNTILD#SLATRDFINWLIQTK 75
Sus scrofa	Query 39 DPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 \|\|\| \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 39 DPLDDPDQMTEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNKNNIAKRHDEFER 97 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTK 82 +\|\|+ \|+\|+ + + \|\|#+ +\|\|+ \|\|++\|\| Sbjct 144 RRHADGSFSDEMNTVLD#NLATRDFINWLLHTK 175
Canis lupus familiaris	Query 39 DPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFER 97 +\|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 39 EPLNDLDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNKNNIAKRHDEFER 97 Query 51 KRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTK 82 +\|\|+ \|+\|+ + + \|\|#+ +\|\|+ \|\|+ \|\| Sbjct 144 RRHADGSFSDEMNTVLD#TLATRDFINWLLQTK 175
Contains: Glucagon; Glucagon-36 (Oxyntomodulin); Glucagon-like peptide	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRN 84 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|#+\|\|\|\|\|\|\|\|\|\|\|+\|\|\|+ Sbjct 1 HSQGTFTNDYSKYLD#TRRAQDFVQWLMSTKRS 32 Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNR 85 \|+ \|\|+\|\|\| \| \|\| # + \|+ \|\| \|\| + \| Sbjct 45 HADGTYTSDVSSYLQ#DQAAKKFVTWLKQGQDRR 77
Heloderma suspectum, venom, Peptide, 39 aa	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMN 80 \| +\|\|\|\|\|\| \|\| ++# + \|++\|\| \| Sbjct 1 HGEGTFTSDLSKQME#EEAVRLFIEWLKN 28
Hydrolagus colliei	Query 39 DPLSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNI--AKRH 92 + +\|+\| + + \|\|\|+ \| \|+\|\|\|\|\|\|\|\|#+\|\| +\|\|\|\|\|\|++\|\|\|\| \| \|\|\| Sbjct 18 ESVSNPSTVTDVKRHTDGIFSSDYSKYLD#NRRTKDFVQWLLSTKRNGANTDKTKRH 73 Query 48 NEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNN--IAKRHDEFER 97 ++ \|\|\|+ \| +\|\|\| + \|# +++ \|\|+ \| \| + +\|+ ++\|\|\| \| \| Sbjct 68 DKTKRHADGIYTSDVASLTD#YLKSKRFVESLSNYNKRQNDRRMSKRHVEGAR 119 Query 49 EDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFE 96 \| \| ++ + +\|+\| \|\|+#++ \|+\|\|+ \|\|+ + \| + \|+ \| \| Sbjct 116 EGARRTEEDYPNDFSSYLE#AKAAKDFIDWLIK-GQGRRDFAEESREIE 162
Squalus acanthias	Query 44 PDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRHDEFE 96 \|++\| \|+\|\|+ \|\|\|\|\|+ + \|\|#+ \|++\|+ \|++\|+\| \| \| \| \| Sbjct 118 PEEM--DRRHADGTFTSEINIVLD#TIAAKEFLNWILNSK----TIQSRDSEIE 164 Query 67 D#SRRAQDFVQWLMNTKRNRNNIAKRHDE 94 \|#+\|\|\|+\|\|\|\|\|\|\|+\|\|\|\| + \|\|\| \| Sbjct 1 D#NRRAKDFVQWLMSTKRNGDK-TKRHAE 27 Query 48 NEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWL--MNTKRNRNNIAKRHDEFER 97 ++ \|\|\|++\|\|+\|\|\| \|# +\|+ \|\| \| \| ++\| +\|+\|\|\| \| \| Sbjct 20 DKTKRHAEGTYTSDVDSLSD#YFKAKRFVDSLTSYNKRQNGRSISKRHVESTR 71 Query 49 EDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNR 85 \| \|\|++\|++ \| \| \|\|+#++ \|+\|\|+ \|\|+ + \| Sbjct 68 ESTRHTEGSYR-DISSYLE#AKAAKDFINWLIKGRGRR 103
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 34 aa	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNR 85 \|+ \|\| \|\|\| \| +\|+# + ++\|+ \|\|++ + \| Sbjct 1 HADGTLTSDISSFLE#KQATKEFIAWLVSGRGRR 33
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 33 aa	Query 56 GTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRN 86 \|\|+\|\|\| \| \|\| # + \|++\|\| \|\| + \|+ Sbjct 3 GTYTSDVSSYLQ#DQAAKEFVSWLKTGRGRRD 33
Candida glabrata	Query 41 LSDPDQMNEDKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAK 90 \|+\| \| \|\|+ \| \| \| \| + +\|#+ \|\| \|+ + \| +\| +\| Sbjct 351 LADGDDDEEDEEDSSSTSESSYDEEID#NEEKQDIVEEEEDADENDDNFSK 400
Ambloplites rupestris	Query 53 HSQGTFTSDYSKYLD#SRRAQDFVQWLMNTKRNRNNIAKRH 92 \|\|\|\|\|\|\|+\|\|+ \|\|+# \|+\|\|\|\|++\|\|\|\| \|\|+ \|\|\| Sbjct 1 HSQGTFTNDYTNYLE#DRQAQDFIRWLMNNKRS-GAAEKRH 39 Query 50 DKRHSQGTFTSDYSKYLD#SRRAQDFVQWLMN--------TKRNRNNIAKRHDE 94 +\|\|\|+ \|\|\|\| \| \| # + +\|\|\| \| + \| \| \| ++\|\| \| Sbjct 36 EKRHADGTFTDDASSDFY#DQAIKDFVAKLKSGQDRREPETDRRREAFSRRHVE 88

[Site 5] RAQDFVQWLM⁷⁹-NTKRNRNNIA

Met⁷⁹ Asn

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Arg⁷⁰	Ala⁷¹	Gln⁷²	Asp⁷³	Phe⁷⁴	Val⁷⁵	Gln⁷⁶	Trp⁷⁷	Leu⁷⁸	Met⁷⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Asn⁸⁰	Thr⁸¹	Lys⁸²	Arg⁸³	Asn⁸⁴	Arg⁸⁵	Asn⁸⁶	Asn⁸⁷	Ile⁸⁸	Ala⁸⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
DKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHAEGTFTSDVSS

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	123.00	10	glucagon, isoform CRA_b
2	N/A	79.70	45	0
3	Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 33 aa	64.70	1	AAB31092.1
4	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 29 aa	61.60	1	glucagon=proglucagon-derived 3.5 kda peptide
5	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 32 aa	60.80	1	glucagon II
6	Contains: Glucagon; Glucagon-like peptide	60.10	4	GLUC_AMICA Glucagon precursor
7	Amia calva=bowfin, pancreatic tissues, Peptide, 29 aa	60.10	1	glucagon
8	Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 29 aa	58.50	1	glucagon G-29
9	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 36 aa	57.80	1	GLUC1_ORENI Glucagon-1 (Glucagon I) gb
10	Lampetra fluviatilis=river lamprey, small intestine, Peptide, 29 aa	56.20	1	GLUC_LAMFL Glucagon gb
11	Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 29 aa	53.50	1	glucagon
12	deshis1, Desphe6, Glu9	53.50	1	A Chain A, Nmr Solution Structure Of The Glucagon
13	Monodelphis domestica	48.90	3	PREDICTED: similar to Gastric inhibitory polypepti
14	Contains: Glucagon-1 (Glucagon I); Glucagon-like peptide 1-I (GLP-1I); Glucagon-like peptide 2-I (GLP-2I)	48.90	1	GLUC1_PETMA Glucagon-1 precursor
15	Xenopus laevis	47.00	5	proglucagon II
16	Hoplobatrachus rugulosus	46.60	1	AF324209_1 proglucagon
17	Bufo marinus	46.60	1	proglucagon
18	Gallus gallus	46.20	4	glucagon isoform 1
19	Meleagris gallopavo	46.20	2	preproglucagon B
20	Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide 1 (GLP-1); Glucagon-like peptide 1(7-37) (GLP-1(7-37)); Glucagon-like peptide 1(7-36) (GLP-1(7-36)); Glucagon-like peptide 2 (GLP-2)	44.70	1	GLUC_HELSU Glucagon precursor
21	Oncorhynchus tshawytscha	43.50	1	S78474_1 proglucagon
22	Ictalurus punctatus	43.10	3	proglucagon I
23	Ambloplites rupestris	43.10	1	proglucagon
24	Rattus norvegicus	42.70	6	Glucose-dependent insulinotropic peptide
25	Canis familiaris	42.70	2	PREDICTED: similar to Gastric inhibitory polypepti
26	Capra hircus	42.70	1	glucagon
27	Pan troglodytes	42.40	2	PREDICTED: fibroblast activation protein, alpha su
28	Xenopus tropicalis	42.40	2	glucagon
29	Danio rerio	42.00	6	hypothetical protein LOC494052
30	Bos taurus	42.00	5	vasoactive intestinal peptide
31	Squalus acanthias	41.60	1	proglucagon
32	Agkistrodon piscivorus	41.60	1	proglucagon
33	Macaca mulatta	41.20	3	PREDICTED: vasoactive intestinal peptide
34	Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 32 aa	41.20	1	glucagon-like peptide, GLP
35	Contains: Glicentin-related polypeptide (GRPP); Glucagon-2 (Glucagon II); Glucagon-like peptide 2 (Glucagon-like peptide II)	41.20	1	GLUC2_LOPAM Glucagon-2 precursor (Glucagon II)
36	Sebastes caurinus	40.80	4	proglucagon I
37	Heloderma suspectum	40.80	2	proglucagon
38	Tetraodon nigroviridis	40.00	3	unnamed protein product
39	Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide	40.00	2	GLUC_CARAU Glucagon precursor
40	Contains: Glicentin-related polypeptide 2 (GRPP 2); Glucagon-2; Glucagon-like peptide 1-2 (GLP 1-2); Glucagon-like peptide 2 (GLP 2)	40.00	1	GLUC2_ONCMY Glucagon-2 precursor (Glucagon II)
41	Contains: Glicentin-related polypeptide 1 (GRPP 1); Glucagon-1; Glucagon-like peptide 1-1 (GLP 1-1); Glucagon-like peptide 2 (GLP 2)	40.00	1	GLUC1_ONCMY Glucagon-1 precursor (Glucagon I)
42	Heloderma horridum	40.00	1	EXE3_HELHO Exendin-3 precursor emb
43	Mus musculus	39.70	6	Gastric inhibitory polypeptide
44	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 33 aa	38.90	1	glucagon-like peptide-2, GLP-2=proglucagon-derived
45	Protopterus dolloi	38.90	1	proglucagon
46	Contains: Intestinal peptide PHI-42; Intestinal peptide PHI-27 (Peptide histidine isoleucinamide 27); Vasoactive intestinal peptide (VIP) (Vasoactive intestinal polypeptide)	38.10	1	VIP_MOUSE VIP peptides precursor
47	Arvicanthis ansorgei	37.40	1	vasoactive intestinal polypeptide
48	synthetic construct	37.00	1	Homo sapiens glucagon
49	Contains: Glucagon-2 (Glucagon II) (Glu II); Glucagon-like peptide 2-II (GLP-2II)	37.00	1	GLUC2_PETMA Glucagon-2 precursor
50	Phodopus sungorus	36.60	1	glucagon precursor
51	Contains: Glicentin-related polypeptide (GRPP); Glucagon-1 (Glucagon I); Glucagon-like peptide 1 (Glucagon-like peptide I)	36.60	1	GLUC1_LOPAM Glucagon-1 precursor (Glucagon I)
52	Sus scrofa	36.20	1	glucagon
53	Neoceratodus forsteri	36.20	1	proglucagon
54	Contains: Glucagon; Glucagon-36 (Oxyntomodulin); Glucagon-like peptide	35.80	1	GLUC_LEPSP Glucagon precursor
55	Canis lupus familiaris	35.80	1	glucagon
56	Heloderma suspectum, venom, Peptide, 39 aa	35.40	1	A Chain A, Solution Structure Of Exendin-4 In 30-V
57	Hydrolagus colliei	35.00	1	proglucagon
58	Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 33 aa	34.70	1	GLUC2_ORENI Glucagon-2 (Glucagon II) gb
59	Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 34 aa	34.70	1	glucagon-like peptide-1, GLP-1=proglucagon-derived

Top-ranked sequences

organism	matching
Homo sapiens	Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSD 106 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSD 106
N/A	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIA 89 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\| Sbjct 1 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNKNNIA 37
Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 33 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRN 84 \|\|+\|\|\|\|\|\|\|\|\|\|+\|+\|\|\|+\|\|\|\|\|\|\|#+\|\|\|\| Sbjct 1 HSEGTFTSDYSKYMDNRRAKDFVQWLM#STKRN 32
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NT 81 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|+\|\|\|\|#+\| Sbjct 1 HSQGTFTSDYSKYLDNRRAQDFIQWLM#ST 29
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 32 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRN 84 \|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\| #\|+\|\|\| Sbjct 1 HSEGTFSNDYSKYLETRRAQDFVQWLK#NSKRN 32
Contains: Glucagon; Glucagon-like peptide	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NT 81 \|\|\|\|\|\|\|+\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|#+\| Sbjct 1 HSQGTFTNDYSKYMDTRRAQDFVQWLM#ST 29
Amia calva=bowfin, pancreatic tissues, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NT 81 \|\|\|\|\|\|\|+\|\|\|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|#+\| Sbjct 1 HSQGTFTNDYSKYMDTRRAQDFVQWLM#ST 29
Scyliorhinus canicula=European common dogfish, pancreas, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NT 81 \|\|+\|\|\|\|\|\|\|\|\|\|+\|+\|\|\|+\|\|\|\|\|\|\|#+\| Sbjct 1 HSEGTFTSDYSKYMDNRRAKDFVQWLM#ST 29
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 36 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRN 84 \|\|+\|\|\|++\|\|\|\|\|\|+ \|+\|\|\|\|\|+\|\|\|#\| \|\|+ Sbjct 1 HSEGTFSNDYSKYLEDRKAQDFVRWLM#NNKRS 32
Lampetra fluviatilis=river lamprey, small intestine, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NT 81 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|++\|+\|\|\| \|\|\|#\|\| Sbjct 1 HSQGSFTSDYSKYLDSKQAKDFVIWLM#NT 29
Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 29 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#N 80 \|\|+\|\|\|\|\|\|\|\|\|\|\|++++\|+\|\|\|+\|\|\|#\| Sbjct 1 HSEGTFTSDYSKYLENKQAKDFVRWLM#N 28
deshis1, Desphe6, Glu9	Query 54 SQGTFTSDYSKYLDSRRAQDFVQWLM#NT 81 \|\|\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\| Sbjct 1 SQGT-TSEYSKYLDSRRAQDFVQWLM#NT 27
Monodelphis domestica	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGT 102 +\|+++\|\|\| \|\|\|\| +\| \|\|\|\| \|\|+#+ \| +\| + \|\|+ \|\| \|+\|\| Sbjct 51 QRYAEGTFISDYSITMDKIMQQDFVNWLL#SQKGKKN--SWRHNITERKADGT 100
Contains: Glucagon-1 (Glucagon I); Glucagon-like peptide 1-I (GLP-1I); Glucagon-like peptide 2-I (GLP-2I)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|++++\|+\|\|\|+\|\|\|#\| \|\| + + + \|\|\|+\|\|\|\|+\|++\| Sbjct 41 KRHSEGTFTSDYSKYLENKQAKDFVRWLM#NAKRGGSELQR------RHADGTFTNDMTS 93 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNR---NNIAKRHDEFERHAE 100 +\|\|+ \|\|\|\|+\| + \|\|\|++ \|+\|\|\| \|\| # + ++\| + + \| +\|\|\|\| Sbjct 80 RRHADGTFTNDMTSYLDAKAARDFVSWLA#RSDKSRRDGGDHLAENSEDKRHAE 132
Xenopus laevis	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVS 108 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|+ +++\|+ ++\|\|\|\|\|\|\|\|\|\|\|\|+ Sbjct 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRS-GGLSRRNADYERHAEGTFTSDVT 107 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 +\|\|++\|\|+\|+\| ++\|\|+ + + \|++\|\|+# \| + + \|\|\|\|\|\|\|\|+\|+++ Sbjct 140 ERHAEGTYTNDVTEYLEEKATKAFIEWLI#KGK-------PKKIRYSRHAEGTFTNDMTN 191 Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRH 92 \|\|++\|\|\|\|+\| + \|\|+ + \|++\|\| \|\|+#\| + \| ++ + \| Sbjct 179 RHAEGTFTNDMTNYLEEKAAKEFVGWLI#NGRPKRKDLLEEH 219
Hoplobatrachus rugulosus	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVS 108 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|+\|\|+ \|+\|\|+ +\|\|\|\|\|\|\|\|+\|+\|\|+ Sbjct 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NSKRS-GGISKRNVQFERHAEGTYTNDVT 107 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 +\|\|++\|\|+\|+\| +++\|+ + \|++\|+ \|\|+# \| + ++ \|\|\|\|\|\|\|\|\|\|++\| Sbjct 95 ERHAEGTYTNDVTQFLEEKAAKEFIDWLI#KGKPKKQRLS-------RHAEGTFTSDMTS 146 Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFE-RHAEGTFTSD 106 \|\|++\|\|\|\|\|\| + \|\|+ + \|++\|\| \|\|+# + \|\| +\| + \|\|\|+\|+\|\|\|\| Sbjct 134 RHAEGTFTSDMTSYLEEKAAKEFVDWLI#KGRPKRNFPDISAEEMDRRHADGSFTSD 189 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NT 81 \|+\|\|+ \|+\|\|\|\|++\| \|\| + \|\|+\|+ \|++#\|\| Sbjct 178 DRRHADGSFTSDFNKALDIKAAQEFLDWII#NT 209
Bufo marinus	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM--#NTKRNRNNIAKRHDEFERHAEGTFTSD 106 \|\|++\|\|\|\|\|\| + +\|+ + \|++\|\| \|\|+ # \|\|\| ++++ \| \|\|\|+\|+\|\|\|\| Sbjct 62 RHAEGTFTSDMTSFLEEKAAKEFVDWLIKG#RPKRNFSDVSAADDMDRRHADGSFTSD 118 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 +\|\|++\|\|+\|+\| +++\|+ + \|++\|+ \|\|+# \| \|+ \|\|\|\|\|\|\|\|\|\|++\| Sbjct 23 ERHAEGTYTNDVTQFLEEKAAKEFIDWLL#------KGIPKK-QRLSRHAEGTFTSDMTS 74 Query 73 DFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVS 108 \|\| \|\|\|\|#\|+\|\|+ +++\|+ +\|\|\|\|\|\|\|\|+\|+\|\|+ Sbjct 1 DFAQWLM#NSKRS-GGMSRRNVQFERHAEGTYTNDVT 35 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NT 81 \|+\|\|+ \|+\|\|\|\|++\| \|\| + \|\|+\|+ \|++#\|\| Sbjct 107 DRRHADGSFTSDFNKALDIKAAQEFLDWII#NT 138
Gallus gallus	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRN------------------RNNIAKRH 92 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#+\|\|\|\| \| \|+\|\|\| Sbjct 53 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#STKRNGQQGQEDKENDKFPDQLSSNAISKRH 112 Query 93 DEFERHAEGTFTSDVSS 109 \|\|\|\|\|\|\|\|\|+\|\|\|++\| Sbjct 113 SEFERHAEGTYTSDITS 129 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAK---RHDEFERHAEGTFTSDV 107 +\|\|++\|\|+\|\|\| + \|\|+ + \|++\|+ \|\|+#\| + \|+ \| + \|\|\|+\|\|\|\|\|\|+ Sbjct 116 ERHAEGTYTSDITSYLEGQAAKEFIAWLV#NGRGRRDFPEKALMAEEMGRRHADGTFTSDI 175 Query 108 S 108 + Sbjct 176 N 176 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNI 88 +\|\|+ \|\|\|\|\|\| +\| \|\| \|++\|++\|\|+#\|\|\| + ++ Sbjct 164 RRHADGTFTSDINKILDDMAAKEFLKWLI#NTKVTQRDL 201
Meleagris gallopavo	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRN------------------RNNIAKRH 92 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#+\|\|\|\| \| \|+\|\|\| Sbjct 53 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#STKRNGQQGQEDKENDEFPDQLSSNAISKRH 112 Query 93 DEFERHAEGTFTSDVSS 109 \|\|\|\|\|\|\|\|\|+\|\|\|++\| Sbjct 113 SEFERHAEGTYTSDITS 129 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAK---RHDEFERHAEGTFTSDV 107 +\|\|++\|\|+\|\|\| + \|\|+ + \|++\|+ \|\|+#\| + \|+ \| + \|\|\|+\|\|\|\|\|\|+ Sbjct 116 ERHAEGTYTSDITSYLEGQAAKEFIAWLV#NGRGRRDFPEKALMAEEMGRRHADGTFTSDI 175 Query 108 S 108 + Sbjct 176 N 176 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNI 88 +\|\|+ \|\|\|\|\|\| +\| \|\| \|++\|++\|\|+#\|\|\| + ++ Sbjct 164 RRHADGTFTSDINKILDDMAAKEFLKWLI#NTKVTQRDL 201
Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide 1 (GLP-1); Glucagon-like peptide 1(7-37) (GLP-1(7-37)); Glucagon-like peptide 1(7-36) (GLP-1(7-36)); Glucagon-like peptide 2 (GLP-2)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKR------------------NRNNIAKRH 92 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\| + \| +\|+ \| Sbjct 51 KRHSQGTFTSDYSKYLDTRRAQDFVQWLM#NTKRSGQQGVEEREKENLLDQLSSNGLARHH 110 Query 93 DEFERHAEGTFTSDVSS 109 \|+\|\|\|\|+\| +\|\|\|+\|\| Sbjct 111 AEYERHADGRYTSDISS 127 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAK---RHDEFERHAEGTFTSD 106 +\|\|+ \| +\|\|\| \| \|\|+ + \|++\|+ \|\|+#\| + \|+ + + \| \|\|\|+\|\|\|\|\|\| Sbjct 114 ERHADGRYTSDISSYLEGQAAKEFIAWLV#NGRGRRDFLEEAGTADDIGRRHADGTFTSD 172 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNI 88 +\|\|+ \|\|\|\|\|\|\|++ \|\| \|+\|++\|\|+#\| \| + ++ Sbjct 162 RRHADGTFTSDYNQLLDDIATQEFLKWLI#NQKVTQRDL 199
Oncorhynchus tshawytscha	Query 54 SQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \| \|\|\|++\|\|\|\|\| + \| \|\|\|\|\|\|\|\|\|#\|+\|\|+ +\|\|\|+\|\|+\|\|\|\|\|+ Sbjct 2 SXGTFSNDYSKYQEERMAQDFVQWLM#NSKRS-------GAPSKRHADGTYTSDVST 50 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNR 85 \|\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|\| \|\| #+ + \| Sbjct 37 KRHADGTYTSDVSTYLQDQAAKDFVSWLK#SGRARR 71
Ictalurus punctatus	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 +\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|#\|+\|\|+ \|\|\|+\|\|+\|\|\|\|\|\| Sbjct 49 RRHSEGTFSNDYSKYLETRRAQDFVQWLM#NSKRS-------DSPARRHADGTYTSDVSS 100 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEF--ERHAEGTFTSDVS 108 +\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|+ \|\| #+ + + + \| + \|\| +\|+\|\|\|\|\|+ Sbjct 87 RRHADGTYTSDVSSYLQDQAAKDFITWLK#SGQPKPDAVETRSVDTLRRRHVDGSFTSDVN 146 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRH 92 +\|\| \|+\|\|\|\| +\| \|\|\| \|++++\|\|+\|#\|+ + + \|\|\| Sbjct 134 RRHVDGSFTSDVNKVLDSLAAKEYLQWVM#NSPSSAS--GKRH 173
Ambloplites rupestris	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|\|\|\|+\|\|+ \|\|+ \|+\|\|\|\|++\|\|\|#\| \| \|+ \|++ \|\|\|+\|\|\|\| \| \|\| Sbjct 1 HSQGTFTNDYTNYLEDRQAQDFIRWLM#NNK--RSGAAEK-----RHADGTFTDDASS 50 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDE--FERHAEGTFTSDV 107 +\|\|\|+ \|\|\|\| \| \| + +\|\|\| \| #+ + \| \| \| \|\| \|\|+\|\|\|\|+ Sbjct 36 EKRHADGTFTDDASSDFYDQAIKDFVAKLK#SGQDRREPETDRRREAFSRRHVEGSFTSDI 95 Query 108 S 108 + Sbjct 96 N 96
Rattus norvegicus	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHA 99 \|+++\|\|\| \|\|\|\| +\| \| \|\|\|\| \|\|+# \| +\|+ +\|+ +\| \| Sbjct 43 RYAEGTFISDYSIAMDKIRQQDFVNWLL#AQKGKKND--WKHNLTQREA 88
Canis familiaris	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHA 99 \|+++\|\|\| \|\|\|\| +\| \| \|\|\|\| \|\|+# \| +\|+ +\|+ +\| \| Sbjct 43 RYAEGTFISDYSIAMDKIRQQDFVNWLL#AQKGKKND--WKHNITQREA 88
Capra hircus	Query 86 NNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 86 NNIAKRHDEFERHAEGTFTSDVSS 109 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNR 85 +\|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|+ \|\|+# + \| Sbjct 11 ERHAEGTFTSDVSSYLEGQAAKEFIAWLV#KGRGRR 45
Pan troglodytes	Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| +++\| \|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 972 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRN-SSVA------YRHAEGTFTSDVSS 1024 Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNR 85 \|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|+ \|\|+# + \| Sbjct 1012 RHAEGTFTSDVSSYLEGQAAKEFIAWLV#KGRGRR 1045
Xenopus tropicalis	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVS 108 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|#\|\|\|\|+ +++\|+ ++\|\|\|\|\|\|\|\|\|\|\|\|+ Sbjct 51 KRHSQGTFTSDYSKYLDSRRAQDFIQWLM#NTKRS-GGLSRRNADYERHAEGTFTSDVT 107 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 +\|\|++\|\|+\|+\| ++\|\|+ + \|++\|++\|\|+#\| \| + + \|\|\|\|\|\|\|\|+\|+++ Sbjct 140 ERHAEGTYTNDVTEYLEEKAAKEFIEWLI#NGK-------PKKIRYSRHAEGTFTNDMTN 191 Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFE---RHAEGTFTSDVS 108 \|\|++\|\|\|\|+\| + \|\|+ + \|++\|\| \|\|+# \| + \| ++ \| \| \|\|\|+\|+\|\|+\|++ Sbjct 179 RHAEGTFTNDMTNYLEEKAAKEFVGWLI#K-GRPKRNFSEAHSVEEMDRRHADGSFTNDIN 237 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTK 82 \|+\|\|+ \|+\|\|+\| +\| \|\| \|\|+\|+ \|++#\|\|+ Sbjct 224 DRRHADGSFTNDINKVLDIIAAQEFLDWVI#NTQ 256
Danio rerio	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|#\| \|\|\| ++ +\|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 48 KRHSEGTFSNDYSKYLETRRAQDFVQWLM#NAKRNGGSV-------KRHAEGTYTSDVSS 99 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWL 78 \|\|\|++\|\|+\|\|\| \| \|\| + \|\| \|\| \|\| Sbjct 86 KRHAEGTYTSDVSSYLQDQAAQSFVAWL 113
Bos taurus	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSD 106 \|\|+ \| \|\|\|\|\|\|+ \| \|+ +++ \|+# \|\| \|+\|++ +\|\|++ \|\| + Sbjct 80 RHADGVFTSDYSRLLGQLSAKKYLESLI#G-KRVSNSISEDQGPIKRHSDAVFTDN 133
Squalus acanthias	Query 67 DSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|+\|\|\|+\|\|\|\|\|\|\|#+\|\|\|\| \|+ +\|\|\|\|\|\|+\|\|\|\| \| Sbjct 1 DNRRAKDFVQWLM#STKRN-------GDKTKRHAEGTYTSDVDS 36 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWL--M#NTKRNRNNIAKRHDEFERHAEGTFTSDVS 108 \|\|\|++\|\|+\|\|\| \| +\|+ \|\| \| #\| ++\| +\|+\|\|\| \| \|\| \|\|++ \|+\| Sbjct 23 KRHAEGTYTSDVDSLSDYFKAKRFVDSLTSY#NKRQNGRSISKRHVESTRHTEGSY-RDIS 81 Query 109 S 109 \| Query 109 S 109 Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRN---------NIAKRHDEFERHAEGT 102 \|\|++\|++ \| \| \|\|+++ \|+\|\|+ \|\|+# + \| \| + \|\|\|+\|\| Sbjct 71 RHTEGSYR-DISSYLEAKAAKDFINWLI#KGRGRREFPEESKEIVNEVIPEEMDRRHADGT 129 Query 103 FTSDVS 108 \|\|\|+++ Sbjct 130 FTSEIN 135 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFE 96 \|+\|\|+ \|\|\|\|\|+ + \|\|+ \|++\|+ \|++#\|+\| \| \| \| \| Sbjct 122 DRRHADGTFTSEINIVLDTIAAKEFLNWIL#NSK----TIQSRDSEIE 164
Agkistrodon piscivorus	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRH---DEFERHAEGTFTSD 106 +\|\|+ \|\|+\|\|\| \| \|\|+ + \|++\|+ \|\|+#\| + \|+ \| \| \|\|\|+\|\|\|\|\|\| Sbjct 34 ERHADGTYTSDISSYLEGQAAKEFIAWLV#NGRGRRDFSEGAHTGEDLGRRHADGTFTSD 92 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNI 88 +\|\|+ \|\|\|\|\|\|\|+\| \|\| \|+\|++\|\|+#\| \| + ++ Sbjct 82 RRHADGTFTSDYNKLLDDIATQEFLKWLI#NQKVTQRDL 119 Query 66 LDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|+ \| ++\|+ \| #+ \| +\|+ \| \|+\|\|\|\|+\|\|+\|\|\|+\|\| Sbjct 9 LEDREKENFLDQLA#S-----NGLARPHAEYERHADGTYTSDISS 47
Macaca mulatta	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSD 106 \|\|+ \| \|\|\|\|+\|\| \| \|+ +++ \|\|# \|\| +\|\|++ +\|\|++ \|\| + Sbjct 80 RHADGVFTSDFSKLLGQLSAKKYLESLM#G-KRVSSNISEDPVPVKRHSDAVFTDN 133
Petromyzon marinus=sea lampreys, intestine, parasitic phase, Peptide, 32 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRN 84 \|+ \|\|\|\|+\| + \|\|\|++ \|+\|\|\| \|\| # + ++ Sbjct 1 HADGTFTNDMTSYLDAKAARDFVSWLA#RSDKS 32
Contains: Glicentin-related polypeptide (GRPP); Glucagon-2 (Glucagon II); Glucagon-like peptide 2 (Glucagon-like peptide II)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\| #\|+\|\|\| \| \|\|\|+\|\|+\|\|\|\|\|\| Sbjct 50 KRHSEGTFSNDYSKYLETRRAQDFVQWLK#NSKRN--------GLFRRHADGTYTSDVSS 100 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWL 78 +\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|\| \|\| Sbjct 87 RRHADGTYTSDVSSYLQDQAAKDFVSWL 114
Sebastes caurinus	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|+\|\|\|++\|\|\|+\|\|+ \|+\|\|\|\|\|+\|\|\|#\| \|\|+ \|\| +\|\|\|+\|\|\|\|\|\|\|\|\| Sbjct 48 KRHSEGTFSNDYSRYLEERKAQDFVRWLM#NNKRS------GADE-KRHADGTFTSDVSS 99 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDE--FERHAEGTFTSDV 107 +\|\|\|+ \|\|\|\|\|\| \| \|\| + +\|\|\| \| #+ + \| + + \| \|\| +\|+\|\|\|\|\| Sbjct 85 EKRHADGTFTSDVSSYLKDQAIKDFVNRLK#SGQVRRESETEWRGEAFSRRHVDGSFTSDV 144 Query 108 S 108 + Sbjct 145 N 145 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDE 94 +\|\| \|+\|\|\|\| +\| \|\|\| \|++++ \|+\|# +\| + + ++ \|+ Sbjct 133 RRHVDGSFTSDVNKVLDSMAAKEYLLWVM#TSKPSGESKKRQEDQ 176
Heloderma suspectum	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKR------------------NRNNIAKRH 92 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\| + \| +\|+ \| Sbjct 51 KRHSQGTFTSDYSKYLDTRRAQDFVQWLM#NTKRSGQQGVEEREKENLLDQLSSNGLARHH 110 Query 93 DEFERHAEGTFTSDVSS 109 \|+\|\|\|\|+\| +\|\|\|+\|\| Sbjct 111 AEYERHADGRYTSDISS 127 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNR 85 +\|\|+ \| +\|\|\| \| \|\|+ + \|++\|+ \|\|+#\| + \| Sbjct 114 ERHADGRYTSDISSYLEGQAAKEFIAWLV#NGRGRR 148
Tetraodon nigroviridis	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\| #\|+\|\|\| + \| \|\|\|+\|\|+\|\|\|\|\|+ Sbjct 787 KRHSEGTFSNDYSKYLETRRAQDFVQWLK#NSKRNGS-------LFRRHADGTYTSDVSA 838 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNR 85 +\|\|+ \|\|+\|\|\| \| \|\| + \|++\|\| \|\| # + \| Sbjct 825 RRHADGTYTSDVSAYLQDQAAKEFVSWLK#TGRGRR 859
Contains: Glicentin-related polypeptide (GRPP); Glucagon; Glucagon-like peptide	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|+\|\|\|++\|\|\|\|\|\|++\|\|\|\|\|\|\|+\|\|\|#\|+\| \| + +\|\|\|\|\|\|+\|\|\|+\|\| Sbjct 48 KRHSEGTFSNDYSKYLETRRAQDFVEWLM#NSKSNGGSA-------KRHAEGTYTSDISS 99 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWL 78 \|\|\|++\|\|+\|\|\| \| +\| + \|\|+\|\| \|\| Sbjct 86 KRHAEGTYTSDISSFLRDQAAQNFVAWL 113
Contains: Glicentin-related polypeptide 2 (GRPP 2); Glucagon-2; Glucagon-like peptide 1-2 (GLP 1-2); Glucagon-like peptide 2 (GLP 2)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\| \|+\|\|\|++ \|\|\|\| + \| \|+\|\|+ \|\|\|#\|+\|\|+ +\|\|\|+\|\|+\|\|\|\|\|+ Sbjct 50 KRQSEGTFSNYYSKYQEERMARDFLHWLM#NSKRS-------GAPSKRHADGTYTSDVST 101 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHD--EFERHAEGTFTSDVS 108 \|\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|\| \|\| #+ \| + + + \|\| +\|+\|\|\|\|\|+ Sbjct 88 KRHADGTYTSDVSTYLQDQAAKDFVSWLK#SGPARRESAEESMNGPMSRRHVDGSFTSDVN 147 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHD 93 +\|\| \|+\|\|\|\| +\| \|\|\| \|++++ \|+\|# +\| + + ++ \| Sbjct 135 RRHVDGSFTSDVNKVLDSLAAKEYLLWVM#TSKTSGKSNKRQED 177
Contains: Glicentin-related polypeptide 1 (GRPP 1); Glucagon-1; Glucagon-like peptide 1-1 (GLP 1-1); Glucagon-like peptide 2 (GLP 2)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|+\|\|\|++\|\|\|\|\| + \| \|\|\|\|\|\|\|\|\|#\|+\|\|+ +\|\|\|+\|\|+\|\|\|\|\|+ Sbjct 50 KRHSEGTFSNDYSKYQEERMAQDFVQWLM#NSKRS-------GAPSKRHADGTYTSDVST 101 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNI--AKRHDEFERHAEGTFTSDVS 108 \|\|\|+ \|\|+\|\|\| \| \|\| + \|+\|\|\| \|\| #+ + \| + ++ \|\| +\|+\|\|\|\|\|+ Sbjct 88 KRHADGTYTSDVSTYLQDQAAKDFVSWLK#SGRARRESAEESRNGPMSRRHVDGSFTSDVN 147 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHD 93 +\|\| \|+\|\|\|\| +\| \|\|\| \|++++ \|+\|# +\| + + ++ \| Sbjct 135 RRHVDGSFTSDVNKVLDSLAAKEYLLWVM#TSKTSGKSNKRQED 177
Heloderma horridum	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#N 80 \|\|\|\| \|\|\|\|\|\| \|\| ++ + \|++\|\| #\| Sbjct 46 KRHSDGTFTSDLSKQMEEEAVRLFIEWLK#N 75
Mus musculus	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHA 99 \|+++\|\|\| \|\|\|\| +\| \| \|\|\|\| \|\|+# + +++ +\|+ +\| \| Sbjct 43 RYAEGTFISDYSIAMDKIRQQDFVNWLL#AQRGKKSD--WKHNITQREA 88
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 33 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NTK 82 \|+ \|+\|\|\|\| +\| \|\|+ \|++\|+ \|\|+#+\|\| Sbjct 1 HADGSFTSDINKVLDTIAAKEFLNWLI#STK 30
Protopterus dolloi	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|\|\|\|\| \|\|\|+\| \|+++ \| \| \|\| \|\|#+\|\|\| ++\|\|\|+\| \|\|\|\|\|\|\|+\|\|\|+\|\| Sbjct 30 KRHSQGTFMSDYAKLLEAKHALDLVQRLM#STKRT-GAVSKRHNEIERHAEGTYTSDISS 87 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFE-RHAEGTFTSDVSS 109 +\|\|++\|\|+\|\|\| \| \|\|+ + +\|++\|\|\|# + \|+ +\| + \|\|\|+\|\| \|\|\|++\| Sbjct 74 ERHAEGTYTSDISSYLEGQAVNEFIRWLM#KGRGRRDFSETDIEEMDRRHADGTVTSDINS 133 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTK 82 \|+\|\|+ \|\| \|\|\| + \|+\| ++\|+ \|\|+#\| \| Sbjct 119 DRRHADGTVTSDINSVLESIATREFLNWLI#NAK 151
Contains: Intestinal peptide PHI-42; Intestinal peptide PHI-27 (Peptide histidine isoleucinamide 27); Vasoactive intestinal peptide (VIP) (Vasoactive intestinal polypeptide)	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSD 106 \|\|+ \| \|\|\|\|\|\|+ \| \|+ +++ \|+# \|\| ++\|++ +\|\|++ \|\| + Sbjct 80 RHADGVFTSDYSRLLGQISAKKYLESLI#G-KRISSSISEDPVPIKRHSDAVFTDN 133
Arvicanthis ansorgei	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSD 106 \|\|+ \| \|\|\|\|\|\|+ \| \|+ +++ \|+# \|\| ++\| + +\|\|++ \|\| + Sbjct 63 RHADGVFTSDYSRLLGQISAKKYLESLI#G-KRISSSILEDPVPIKRHSDAVFTDN 116
synthetic construct	Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRN---NIAKRHDEFERHAEGTFTSDV 107 +\|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|+ \|\|+# + \|+ +\| + \|\|\|+\|+\|+ ++ Sbjct 96 ERHAEGTFTSDVSSYLEGQAAKEFIAWLV#KGRGRRDFPEEVAIVEELGRRHADGSFSDEM 155 Query 108 SS 109 ++ Sbjct 156 NT 157 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTK 82 +\|\|+ \|+\|+ + + \|\|+ \|+\|\|+ \|\|+# \|\| Sbjct 144 RRHADGSFSDEMNTILDNLAARDFINWLI#QTK 175
Contains: Glucagon-2 (Glucagon II) (Glu II); Glucagon-like peptide 2-II (GLP-2II)	Query 52 RHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVS 108 \|\|\|\|\|+\|\|\|\|\|\|\|+\|\| ++\|+\|\|\| \|\|+#\|\|\|\| + + +\|\|++\|+\|\|+\|++ Sbjct 43 RHSQGSFTSDYSKHLDVKQAKDFVTWLL#NTKRRGVDAQAGANLEKRHSDGSFTNDMN 99 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKR 83 +\|\|\|\| \|+\|\|+\| + \|\| \|++\|++\|\| # \| Sbjct 86 EKRHSDGSFTNDMNVMLDRMSAKNFLEWLK#QQGR 119
Phodopus sungorus	Query 56 GTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRN---NIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|\|\| \| \|\|+ + \|++\|+ \|\|+# + \|+ + + \|\|\|+\|+\|+ ++++ Sbjct 1 GTFTSDVSSYLEGQAAKEFIAWLV#KGRGRRDFPEEVTIVEELGRRHADGSFSDEMNT 57 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTK 82 +\|\|+ \|+\|+ + + \|\|\| +\|\|+ \|\|+# \|\| Sbjct 44 RRHADGSFSDEMNTILDSLATRDFINWLI#QTK 75
Contains: Glicentin-related polypeptide (GRPP); Glucagon-1 (Glucagon I); Glucagon-like peptide 1 (Glucagon-like peptide I)	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|+\|\|\|++\|\|\|\|\|\|+ \|+\|\|+\|\|+\|\|\|#\| \| \|+ +\|++ \|\|\|+\|\|\|\|\|\|\|\|\| Sbjct 51 KRHSEGTFSNDYSKYLEDRKAQEFVRWLM#NNK--RSGVAEK-----RHADGTFTSDVSS 102 Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWL 78 +\|\|\|+ \|\|\|\|\|\| \| \|\| + +\|\|\| \| Sbjct 88 EKRHADGTFTSDVSSYLKDQAIKDFVDRL 116
Sus scrofa	Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNKNNIAKRHDEFERHAEGTFTSDVSS 109 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRN---NIAKRHDEFERHAEGTFTSDV 107 +\|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|+ \|\|+# + \|+ + + \|\|\|+\|+\|+ ++ Sbjct 96 ERHAEGTFTSDVSSYLEGQAAKEFIAWLV#KGRGRRDFPEEVTIVEELRRRHADGSFSDEM 155 Query 108 SS 109 ++ Sbjct 156 NT 157 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTK 82 +\|\|+ \|+\|+ + + \|\|+ +\|\|+ \|\|+#+\|\| Sbjct 144 RRHADGSFSDEMNTVLDNLATRDFINWLL#HTK 175
Neoceratodus forsteri	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|\|\|\|\| \|\|\|+\| \|++\| \| \|\|\|\| \|\|#\|\|\|\|\| ++\|\|\| \| \|\|\|\|\|\|\|+\|\|\|+\|\| Sbjct 22 KRHSQGTFMSDYAKLLEARHALDFVQRLM#NTKRN-GGVSKRHSEIERHAEGTYTSDISS 79 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEF-ERHAEGTFTSDVSS 109 +\|\|++\|\|+\|\|\| \| \|\|+ + \| +\|\|+\|\|+# + \|+ +\| \|\|\|+\|\| \|+\|+++ Sbjct 66 ERHAEGTYTSDISSYLEGQAANEFVRWLL#KGRGRRDFSDTDAEEMGRRHADGTITNDMNN 125 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTK 82 +\|\|+ \|\| \|+\| + \|+\| ++\|+ \|\|+#\|+\| Sbjct 112 RRHADGTITNDMNNVLESIATREFLNWLI#NSK 143
Contains: Glucagon; Glucagon-36 (Oxyntomodulin); Glucagon-like peptide	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|#+\|\|\|+ \| \|\|+\|\|+\|\|\|\|\|\| Sbjct 1 HSQGTFTNDYSKYLDTRRAQDFVQWLM#STKRS-GGITXXXXXXXXHADGTYTSDVSS 56 Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNR 85 \|+ \|\|+\|\|\| \| \|\| + \|+ \|\| \|\| # + \| Sbjct 45 HADGTYTSDVSSYLQDQAAKKFVTWLK#QGQDRR 77
Canis lupus familiaris	Query 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 50 DKRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNKNNIAKRHDEFERHAEGTFTSDVSS 109 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRN---NIAKRHDEFERHAEGTFTSDV 107 +\|\|++\|\|\|\|\|\| \| \|\|+ + \|++\|+ \|\|+# + \|+ +\| + \|\|\|+\|+\|+ ++ Sbjct 96 ERHAEGTFTSDVSSYLEGQAAKEFIAWLV#KGRGRRDFPEEVAIVEEFRRRHADGSFSDEM 155 Query 108 SS 109 ++ Sbjct 156 NT 157 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTK 82 +\|\|+ \|+\|+ + + \|\|+ +\|\|+ \|\|+# \|\| Sbjct 144 RRHADGSFSDEMNTVLDTLATRDFINWLL#QTK 175
Heloderma suspectum, venom, Peptide, 39 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#N 80 \| +\|\|\|\|\|\| \|\| ++ + \|++\|\| #\| Sbjct 1 HGEGTFTSDLSKQMEEEAVRLFIEWLK#N 28
Hydrolagus colliei	Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFERHAEGTFTSDVSS 109 \|\|\|+ \| \|+\|\|\|\|\|\|\|\|+\|\| +\|\|\|\|\|\|+#+\|\|\|\| \| \|+ +\|\|\|+\| +\|\|\|\|+\| Sbjct 30 KRHTDGIFSSDYSKYLDNRRTKDFVQWLL#STKRNGANT----DKTKRHADGIYTSDVAS 84 Query 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRNN--IAKRHDEFERHAEGTFTSDVS 108 \|\|\|+ \| +\|\|\| + \| +++ \|\|+ \| #\| + +\|+ ++\|\|\| \| \| \| + +\| \| Sbjct 71 KRHADGIYTSDVASLTDYLKSKRFVESLS#NYNKRQNDRRMSKRHVEGARRTEEDYPNDFS 130 Query 109 S 109 \| Query 109 S 109 Query 51 KRHSQGT------FTSDYSKYLDSRRAQDFVQWLM#NTKRNRNNIAKRHDEFE 96 \|\|\| +\| + +\|+\| \|\|+++ \|+\|\|+ \|\|+# + \| + \|+ \| \| Sbjct 112 KRHVEGARRTEEDYPNDFSSYLEAKAAKDFIDWLI#K-GQGRRDFAEESREIE 162
Tilapia nilotica=Nile tilapia, Brockmann body, Peptide, 33 aa	Query 56 GTFTSDYSKYLDSRRAQDFVQWLM#NTKRNRN 86 \|\|+\|\|\| \| \|\| + \|++\|\| \|\| # + \|+ Sbjct 3 GTYTSDVSSYLQDQAAKEFVSWLK#TGRGRRD 33
Amphiuma tridactylum=three-toed amphiumae, pancreas, Peptide, 34 aa	Query 53 HSQGTFTSDYSKYLDSRRAQDFVQWLM#NTKRNR 85 \|+ \|\| \|\|\| \| +\|+ + ++\|+ \|\|+#+ + \| Sbjct 1 HADGTLTSDISSFLEKQATKEFIAWLV#SGRGRR 33

* References

[PubMed ID: 18996102] Ruano G, Bernene J, Windemuth A, Bower B, Wencker D, Seip RL, Kocherla M, Holford TR, Petit WA, Hanks S, Physiogenomic comparison of edema and BMI in patients receiving rosiglitazone or pioglitazone. Clin Chim Acta. 2009 Feb;400(1-2):48-55. Epub 2008 Oct 26.

» More (11) « Hide Below

[PubMed ID: 19077438] ... Chung WK, Patki A, Matsuoka N, Boyer BB, Liu N, Musani SK, Goropashnaya AV, Tan PL, Katsanis N, Johnson SB, Gregersen PK, Allison DB, Leibel RL, Tiwari HK, Analysis of 30 genes (355 SNPS) related to energy homeostasis for association with adiposity in European-American and Yup'ik Eskimo populations. Hum Hered. 2009;67(3):193-205. Epub 2008 Dec 15.

[PubMed ID: 18832087] ... Liu X, Murali SG, Holst JJ, Ney DM, Enteral nutrients potentiate the intestinotrophic action of glucagon-like peptide-2 in association with increased insulin-like growth factor-I responses in rats. Am J Physiol Regul Integr Comp Physiol. 2008 Dec;295(6):R1794-802. Epub

[PubMed ID: 18760331] ... Qin Z, Sun Z, Huang J, Hu Y, Wu Z, Mei B, Mutated recombinant human glucagon-like peptide-1 protects SH-SY5Y cells from apoptosis induced by amyloid-beta peptide (1-42). Neurosci Lett. 2008 Oct 31;444(3):217-21. Epub 2008 Aug 22.

[PubMed ID: 18298441] ... Hellstrom PM, Naslund E, Edholm T, Schmidt PT, Kristensen J, Theodorsson E, Holst JJ, Efendic S, GLP-1 suppresses gastrointestinal motility and inhibits the migrating motor complex in healthy subjects and patients with irritable bowel syndrome. Neurogastroenterol Motil. 2008 Jun;20(6):649-59. Epub 2008 Feb 19.

[PubMed ID: 11278902] ... Bertenshaw GP, Turk BE, Hubbard SJ, Matters GL, Bylander JE, Crisman JM, Cantley LC, Bond JS, Marked differences between metalloproteases meprin A and B in substrate and peptide bond specificity. J Biol Chem. 2001 Apr 20;276(16):13248-55. Epub 2001 Jan 22.

[PubMed ID: 2753890] ... Orskov C, Bersani M, Johnsen AH, Hojrup P, Holst JJ, Complete sequences of glucagon-like peptide-1 from human and pig small intestine. J Biol Chem. 1989 Aug 5;264(22):12826-9.

[PubMed ID: 2901414] ... Drucker DJ, Asa S, Glucagon gene expression in vertebrate brain. J Biol Chem. 1988 Sep 25;263(27):13475-8.

[PubMed ID: 3569278] ... Novak U, Wilks A, Buell G, McEwen S, Identical mRNA for preproglucagon in pancreas and gut. Eur J Biochem. 1987 May 4;164(3):553-8.

[PubMed ID: 3725587] ... White JW, Saunders GF, Structure of the human glucagon gene. Nucleic Acids Res. 1986 Jun 25;14(12):4719-30.

[PubMed ID: 6546710] ... Schroeder WT, Lopez LC, Harper ME, Saunders GF, Localization of the human glucagon gene (GCG) to chromosome segment 2q36----37. Cytogenet Cell Genet. 1984;38(1):76-9.

[PubMed ID: 7340337] ... Kargel HJ, Dettmer R, Etzold G, Kirschke H, Bohley P, Langner J, Action of rat liver cathepsin L on glucagon. Acta Biol Med Ger. 1981;40(9):1139-43.