XSB2613 : RecName: Full=Vimentin
[ CaMP Format ]
This entry is computationally expanded from SB0037
* Basic Information
| Organism | Pan troglodytes (chimpanzee) |
| Protein Names | Vimentin |
| Gene Names | VIM |
| Gene Locus | Not available |
| GO Function | Not available |
| Entrez Protein | Entrez Nucleotide | Entrez Gene | UniProt | OMIM | HGNC | HPRD | KEGG |
|---|---|---|---|---|---|---|---|
| Q5R1W8 | N/A | N/A | VIME_PANTR | N/A | N/A | N/A | ptr:493952 |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 466 aa
Average Mass: 53.653 kDa
Monoisotopic Mass: 53.620 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| Filament_head 1. | 6 | 101 | 125.4 | 1.8e-34 |
| --- cleavage 91 (inside Filament_head 6..101) --- | ||||
| --- cleavage 63 (inside Filament_head 6..101) --- | ||||
| Filament 1. | 102 | 410 | 612.7 | 3.8e-181 |
| --- cleavage 264 (inside Filament 102..410) --- | ||||
3. Sequence Information
Fasta Sequence: XSB2613.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
3 [sites]
Cleavage sites (±10aa)
[Site 1] DSVDFSLADA91-INTEFKNTRT
Ala91 
Ile
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Asp82 | Ser83 | Val84 | Asp85 | Phe86 | Ser87 | Leu88 | Ala89 | Asp90 | Ala91 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ile92 | Asn93 | Thr94 | Glu95 | Phe96 | Lys97 | Asn98 | Thr99 | Arg100 | Thr101 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| ATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKV |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Homo sapiens | 117.00 | 36 | VIM |
| 2 | N/A | 117.00 | 25 | - |
| 3 | Canis familiaris | 117.00 | 18 | PREDICTED: similar to vimentin isoform 1 |
| 4 | Macaca mulatta | 117.00 | 7 | PREDICTED: vimentin |
| 5 | Macaca fascicularis | 117.00 | 6 | VIME_MACFA Vimentin sp |
| 6 | Pan troglodytes | 117.00 | 6 | vimentin |
| 7 | synthetic construct | 117.00 | 2 | vimentin |
| 8 | Pan troglodytes verus | 117.00 | 1 | vimentin |
| 9 | Mus musculus | 115.00 | 30 | vimentin |
| 10 | Bos taurus | 115.00 | 11 | vimentin |
| 11 | Ovis aries | 115.00 | 2 | vimentin |
| 12 | Rattus norvegicus | 114.00 | 10 | vimentin |
| 13 | Monodelphis domestica | 110.00 | 6 | PREDICTED: similar to vimentin |
| 14 | Gallus gallus | 91.30 | 5 | vimentin |
| 15 | Acipenser baerii | 88.20 | 2 | vimentin |
| 16 | Xenopus tropicalis | 82.40 | 8 | Unknown (protein for IMAGE:7605834) |
| 17 | Xenopus laevis | 82.00 | 21 | Vim1 protein |
| 18 | Daboia russellii | 82.00 | 1 | AF447708_1 vimentin |
| 19 | Oncorhynchus mykiss | 80.50 | 2 | VIME_ONCMY Vimentin emb |
| 20 | Cyprinus carpio | 77.40 | 1 | VIME_CYPCA Vimentin gb |
| 21 | Danio rerio | 76.60 | 24 | vimentin |
| 22 | Protopterus aethiopicus | 73.90 | 2 | type III desmin |
| 23 | Sus scrofa | 72.00 | 3 | desmin |
| 24 | Tetraodon nigroviridis | 70.90 | 7 | unnamed protein product |
| 25 | Cricetulus griseus | 70.50 | 1 | vimentin |
| 26 | Lampetra fluviatilis | 68.90 | 1 | intermediate filament protein type III |
| 27 | Canis lupus familiaris | 68.20 | 1 | desmin |
| 28 | Mesocricetus auratus | 67.40 | 1 | DESM_MESAU Desmin gb |
| 29 | Takifugu rubripes | 62.80 | 1 | TPA: desmin |
| 30 | Scyliorhinus stellaris | 62.40 | 2 | vimentin |
| 31 | Torpedo californica | 61.20 | 1 | IF3T_TORCA Type III intermediate filament emb |
| 32 | Pongo pygmaeus | 57.00 | 1 | hypothetical protein |
| 33 | Petromyzon marinus | 49.30 | 3 | neurofilament subunit NF-95 |
| 34 | Styela clava | 46.20 | 1 | intermediate filament protein A |
Top-ranked sequences
| organism | matching |
|---|---|
| Homo sapiens | Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |
| N/A | Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |
| Canis familiaris | Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |
| Macaca mulatta | Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |
| Macaca fascicularis | Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |
| Pan troglodytes | Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |
| synthetic construct | Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |
| Pan troglodytes verus | Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |
| Mus musculus | Query 63 TRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 63 TRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |
| Bos taurus | Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 ||||||||||| ||||||||||||||||||#|||||||||||||||||||||||||||||| Sbjct 62 ATRSSAVRLRSGVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |
| Ovis aries | Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 ||||||||||| ||||||||||||||||||#|||||||||||||||||||||||||||||| Sbjct 62 ATRSSAVRLRSGVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |
| Rattus norvegicus | Query 63 TRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |||||||||||+|||||||||||||||||#|||||||||||||||||||||||||||||| Sbjct 63 TRSSAVRLRSSMPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |
| Monodelphis domestica | Query 62 ATRSSAVRLRSS--VPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYID 119 |||||| ||||| +|||||||||||||||||#|||||||||||||||||||||||||||| Sbjct 61 ATRSSAARLRSSAPIPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYID 120 Query 120 KV 121 || Query 120 KV 121 |
| Gallus gallus | Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 ||++++||||||+| +|+ |+|||+||||#|||||| |||||||||||||||||||||| Sbjct 37 ATKATSVRLRSSMPPMRM-HDAVDFTLADA#INTEFKANRTNEKVELQELNDRFANYIDKV 95 |
| Acipenser baerii | Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 ||+||+ ||||| | ||| +++||+|+||#|||||| ||||| |+| ||||||+||||| Sbjct 52 ATKSSSTRLRSSAPQTRLLSETLDFALSDA#INTEFKANRTNEKAEMQHLNDRFASYIDKV 111 |
| Xenopus tropicalis | Query 64 RSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 +||+||||||+| || |+|||+||||#+| |+| ||||| |+|||||||||+|||| Sbjct 84 KSSSVRLRSSLPPARLA-DTVDFTLADA#VNLEYKANRTNEKAEMQELNDRFANFIDKV 140 |
| Xenopus laevis | Query 64 RSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 +||+||||||+| |+ |||||+||||#+| ||| ||||| |+ ||||||||+|||| Sbjct 58 KSSSVRLRSSLPPARMA-DSVDFALADA#VNLEFKANRTNEKAEMMELNDRFANFIDKV 114 |
| Daboia russellii | Query 65 SSAVRLRSSVPGVRLLQDS-VDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 | |+|+||| | +| |+ ||||||||#|||||| ||||| |||||||||||||||| Sbjct 59 SKAMRVRSSFPPMRTGGDAMVDFSLADA#INTEFKANRTNEKAELQELNDRFANYIDKV 116 |
| Oncorhynchus mykiss | Query 65 SSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 | |||||| | || |+|||+|+||#||+||| ||||| |+| ||||||+||||| Sbjct 61 SKNVRLRSSAPMPRLSSDTVDFALSDA#INSEFKANRTNEKAEMQHLNDRFASYIDKV 117 |
| Cyprinus carpio | Query 65 SSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 | |+||| | || +++|| ||||#|||||| ||||| |+| ||||||+||||| Sbjct 56 SKGARVRSSGPLPRLATETLDFGLADA#INTEFKTNRTNEKAEMQHLNDRFASYIDKV 112 |
| Danio rerio | Query 65 SSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 | +|+|| | || |++|| ||+|#|||||| ||||| |+| ||||||+||||| Sbjct 56 SKGLRVRSGAPLPRLATDTLDFGLAEA#INTEFKANRTNEKAEMQHLNDRFASYIDKV 112 |
| Protopterus aethiopicus | Query 63 TRSSAVRLRSS-----VPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANY 117 +||||| || +| + +|+|||||||#+| || |||||| |||||||||||| Sbjct 47 SRSSAVPSYSSFRATRIPAAKTALESMDFSLADA#MNQEFIQTRTNEKAELQELNDRFANY 106 Query 118 IDKV 121 |+|| Sbjct 107 IEKV 110 |
| Sus scrofa | Query 62 ATRSSAVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |+| | |+ || | | +|||||||#+| || ||||||||||||||||||||+|| Sbjct 62 ASRLGATRVPSSSYGAGEL---LDFSLADA#VNQEFLTTRTNEKVELQELNDRFANYIEKV 118 |
| Tetraodon nigroviridis | Query 62 ATRSS-AVRLRSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDK 120 |+||| | ||| || | |||+||+|#+| || ||+||| ||||||||||++|+| Sbjct 49 ASRSSYQQRSRSSAQAPRLSYDKVDFTLAEA#VNQEFLTTRSNEKAELQELNDRFASFIEK 108 Query 121 V 121 | Query 121 V 121 |
| Cricetulus griseus | Query 87 SLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 |||||# ||||||||||||||||||||||||||||| Sbjct 1 SLADA#CNTEFKNTRTNEKVELQELNDRFANYIDKV 35 |
| Lampetra fluviatilis | Query 72 SSVPGVRLLQ-DSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 || |+ | +||||||||#+| +++ |||||||||||||||||+||||| Sbjct 75 SSASYVKALSGQNVDFSLADA#LNADYRTTRTNEKVELQELNDRFASYIDKV 125 |
| Canis lupus familiaris | Query 84 VDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 +|||||||#+| || ||||||||||||||||||||+|| Sbjct 88 LDFSLADA#VNQEFLTTRTNEKVELQELNDRFANYIEKV 125 |
| Mesocricetus auratus | Query 84 VDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 +|||||||#+| || ||||||||||||||||||||+|| Sbjct 88 LDFSLADA#VNQEFLATRTNEKVELQELNDRFANYIEKV 125 |
| Takifugu rubripes | Query 82 DSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 + +||+||||#+| +| ||||||| ||| ||||||+||+|| Sbjct 61 EKLDFNLADA#MNQDFLNTRTNEKAELQHLNDRFASYIEKV 100 |
| Scyliorhinus stellaris | Query 71 RSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 ||| ||+ + +|++||| ||#+|+||| |+||| |+ ||||| ||++||| Sbjct 58 RSSAPGIAM-GNSLNFSLVDA#MNSEFKVNRSNEKAEMIELNDRLANFLDKV 107 |
| Torpedo californica | Query 65 SSAVRL-RSSVPGVRLLQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 | +||| ||| | | ++||+| ||#+|+||| ||||| |+ ||||| ||++||| Sbjct 59 SRSVRLVRSSAPVVAS-SSNLDFTLVDA#MNSEFKVNRTNEKAEMIELNDRLANFLDKV 115 |
| Pongo pygmaeus | Query 75 PGVRL--------LQDSVDFSLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 || || | |||||| |#+| || || +|+ |+ |||||||+||+|| Sbjct 33 PGTRLSLARMPPPLPTRVDFSLAGA#LNAGFKETRASERAEMMELNDRFASYIEKV 87 |
| Petromyzon marinus | Query 73 SVPGVRLLQDSVDF-SLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 | | + ||+|| | + # + +||++| ||| ||+|||||| ||+|| Sbjct 69 STPALNSAADSLDFMSQSSV#FSGDFKHSRVNEKQLLQDLNDRFAGYIEKV 118 |
| Styela clava | Query 87 SLADA#INTEFKNTRTNEKVELQELNDRFANYIDKV 121 +| |+#+ |||+ | ||| |||+|| ||| || || Sbjct 87 ALEDS#MGTEFQGQRINEKAELQQLNSRFAEYITKV 121 |
[Site 2] ASSPGGVYAT63-RSSAVRLRSS
Thr63 Arg
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Ala54 | Ser55 | Ser56 | Pro57 | Gly58 | Gly59 | Val60 | Tyr61 | Ala62 | Thr63 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Arg64 | Ser65 | Ser66 | Ala67 | Val68 | Arg69 | Leu70 | Arg71 | Ser72 | Ser73 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| STRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAIN |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Homo sapiens | 115.00 | 8 | VIM |
| 2 | Macaca fascicularis | 115.00 | 2 | VIME_MACFA Vimentin sp |
| 3 | synthetic construct | 115.00 | 1 | vimentin |
| 4 | Pan troglodytes verus | 115.00 | 1 | vimentin |
| 5 | Pan troglodytes | 115.00 | 1 | vimentin |
| 6 | Macaca mulatta | 115.00 | 1 | PREDICTED: vimentin |
| 7 | Canis familiaris | 114.00 | 12 | PREDICTED: similar to vimentin isoform 1 |
| 8 | N/A | 114.00 | 10 | - |
| 9 | Mus musculus | 111.00 | 14 | unnamed protein product |
| 10 | Ovis aries | 111.00 | 2 | vimentin |
| 11 | Bos taurus | 110.00 | 2 | vimentin |
| 12 | Rattus norvegicus | 110.00 | 1 | vimentin |
| 13 | Monodelphis domestica | 97.40 | 1 | PREDICTED: similar to vimentin |
| 14 | Gallus gallus | 72.40 | 2 | vimentin |
| 15 | Xenopus laevis | 59.30 | 8 | VIM1_XENLA Vimentin-1/2 emb |
| 16 | Daboia russellii | 57.80 | 1 | AF447708_1 vimentin |
| 17 | Acipenser baerii | 56.20 | 1 | vimentin |
| 18 | Xenopus tropicalis | 54.70 | 3 | Unknown (protein for IMAGE:7605834) |
| 19 | Oncorhynchus mykiss | 45.80 | 1 | VIME_ONCMY Vimentin emb |
| 20 | Danio rerio | 45.40 | 6 | vimentin |
| 21 | Tetraodon nigroviridis | 37.70 | 2 | unnamed protein product |
| 22 | Cyprinus carpio | 36.60 | 1 | VIME_CYPCA Vimentin gb |
Top-ranked sequences
| organism | matching |
|---|---|
| Homo sapiens | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |
| Macaca fascicularis | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |
| synthetic construct | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |
| Pan troglodytes verus | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |
| Pan troglodytes | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |
| Macaca mulatta | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |
| Canis familiaris | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |||||||||||||||||||||||||| |||#|||||||||||||||||||||||||||||| Sbjct 34 STRTYSLGSALRPSTSRSLYASSPGGAYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |
| N/A | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |||||||| |||||||||||||||||||||#|||||||||||||||||||||||||||||| Sbjct 34 STRTYSLGDALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |
| Mus musculus | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 ||||||||||||||||||||+||||| | |#|||||||||||||||||||||||||||||| Sbjct 34 STRTYSLGSALRPSTSRSLYSSSPGGAYVT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |
| Ovis aries | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |||||||||||||||||+|| |||||||||#||||||||| |||||||||||||||||||| Sbjct 34 STRTYSLGSALRPSTSRTLYTSSPGGVYAT#RSSAVRLRSGVPGVRLLQDSVDFSLADAIN 93 |
| Bos taurus | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |||||||||||||+|||+|| |||||||||#||||||||| |||||||||||||||||||| Sbjct 34 STRTYSLGSALRPTTSRTLYTSSPGGVYAT#RSSAVRLRSGVPGVRLLQDSVDFSLADAIN 93 |
| Rattus norvegicus | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 ||||||||||||||||||||+||||| | |#||||||||||+||||||||||||||||||| Sbjct 34 STRTYSLGSALRPSTSRSLYSSSPGGAYVT#RSSAVRLRSSMPGVRLLQDSVDFSLADAIN 93 |
| Monodelphis domestica | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSS--VPGVRLLQDSVDFSLADA 91 || ||||||+||||+||+|+|||| ||||#|||| ||||| +||||||||||||||||| Sbjct 33 STTRYSLGSAIRPSTTRSVYSSSPGAVYAT#RSSAARLRSSAPIPGVRLLQDSVDFSLADA 92 Query 92 IN 93 || Query 92 IN 93 |
| Gallus gallus | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |+ ||||||||||++| + || |||||||#++++||||||+| +| + |+|||+|||||| Sbjct 10 SSTRYSLGSALRPSSARYVSAS-PGGVYAT#KATSVRLRSSMPPMR-MHDAVDFTLADAIN 67 |
| Xenopus laevis | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |+ |+||||+||||| + |+ #+||+||||||+| | + |||||+||||+| Sbjct 28 SSTRYTLGSAMRPSTSSRMVYSTSSSPAVF#KSSSVRLRSSLPPAR-MADSVDFALADAVN 86 |
| Daboia russellii | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDS-VDFSLADAI 92 || |||||++|||+ | | |||+|| # | |+|+||| | +| |+ ||||||||| Sbjct 32 STTRYSLGSSIRPSSVR--LVSCPGGIYA-#-SKAMRVRSSFPPMRTGGDAMVDFSLADAI 87 Query 93 N 93 | Query 93 N 93 |
| Acipenser baerii | Query 34 STRTYSLGSALRPSTSRSLYAS-SPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAI 92 |||+|| | || | | | +|||#+||+ ||||| | ||| +++||+|+||| Sbjct 23 STRSYSSRQYSSPIRSRVSYTSHSAPTLYAT#KSSSTRLRSSAPQTRLLSETLDFALSDAI 82 Query 93 N 93 | Query 93 N 93 |
| Xenopus tropicalis | Query 34 STRTYSLGSALRP----STSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLA 89 |+ ||||||+|| |+|| +|++| #+||+||||||+| | | |+|||+|| Sbjct 51 SSTRYSLGSAMRPGAGSSSSRVVYSTSASPA-VF#KSSSVRLRSSLPPAR-LADTVDFTLA 108 Query 90 DAIN 93 ||+| Sbjct 109 DAVN 112 |
| Oncorhynchus mykiss | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |+| || | +| |+ | |+| +||+#++ |||||| | || |+|||+|+|||| Sbjct 35 SSRQYS--SPVR-SSRMSYSVSAPPSIYAS#KN--VRLRSSAPMPRLSSDTVDFALSDAIN 89 |
| Danio rerio | Query 34 STRTYSLGSALRPSTSRSLY--ASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADA 91 |+| || | +| |+||+ | +|+| ||| # | +|+|| | || |++|| ||+| Sbjct 27 SSRQYS--SPVRVSSSRTSYNYSSAPPSVYA-#-SKGLRVRSGAPLPRLATDTLDFGLAEA 82 Query 92 IN 93 || Query 92 IN 93 |
| Tetraodon nigroviridis | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSS-AVRLRSSVPGVRLLQDSVDFSLADAI 92 | +|| |+ | | | | | | |+#||| | ||| || | |||+||+|+ Sbjct 22 SVSSYSPVSS-RMSASGGRYMRSASPVVAS#RSSYQQRSRSSAQAPRLSYDKVDFTLAEAV 80 Query 93 N 93 | Query 93 N 93 |
| Cyprinus carpio | Query 34 STRTYSLGSALRPSTSRSLYASSPGGVYAT#RSSAVRLRSSVPGVRLLQDSVDFSLADAIN 93 |+| || | +| ++ | ++| # | |+||| | || +++|| |||||| Sbjct 27 SSRQYS--SPVRTTSRVSYSSASSASPSIY#MSKGARVRSSGPLPRLATETLDFGLADAIN 84 |
[Site 3] QIDVDVSKPD264-LTAALRDVRQ
Asp264 Leu
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Gln255 | Ile256 | Asp257 | Val258 | Asp259 | Val260 | Ser261 | Lys262 | Pro263 | Asp264 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Leu265 | Thr266 | Ala267 | Ala268 | Leu269 | Arg270 | Asp271 | Val272 | Arg273 | Gln274 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| KKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSK |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Homo sapiens | 83.60 | 46 | vimentin |
| 2 | Mus musculus | 83.60 | 28 | unnamed protein product |
| 3 | N/A | 83.60 | 22 | - |
| 4 | Rattus norvegicus | 83.60 | 11 | vimentin |
| 5 | Macaca mulatta | 83.60 | 10 | PREDICTED: vimentin |
| 6 | Pan troglodytes | 83.60 | 8 | vimentin |
| 7 | Macaca fascicularis | 83.60 | 4 | VIME_MACFA Vimentin sp |
| 8 | synthetic construct | 83.60 | 3 | vimentin |
| 9 | Cricetulus griseus | 83.60 | 1 | vimentin |
| 10 | Pan troglodytes verus | 83.60 | 1 | vimentin |
| 11 | Canis familiaris | 82.40 | 20 | PREDICTED: similar to vimentin isoform 1 |
| 12 | Bos taurus | 82.40 | 8 | vimentin |
| 13 | Bubalus bubalis | 82.40 | 1 | vimentin |
| 14 | Sus scrofa domestica | 82.40 | 1 | vimentin |
| 15 | Monodelphis domestica | 82.00 | 6 | PREDICTED: similar to vimentin |
| 16 | Gallus gallus | 82.00 | 4 | vimentin |
| 17 | Ovis aries | 80.90 | 2 | vimentin |
| 18 | Daboia russellii | 79.30 | 1 | AF447708_1 vimentin |
| 19 | Xenopus laevis | 77.40 | 15 | vimentin 1 |
| 20 | Bos grunniens | 77.00 | 1 | vimentin |
| 21 | Xenopus tropicalis | 76.30 | 5 | Unknown (protein for IMAGE:7605834) |
| 22 | Cyprinus carpio | 72.80 | 3 | VIME_CYPCA Vimentin gb |
| 23 | Sus scrofa | 72.40 | 3 | desmin |
| 24 | Canis lupus familiaris | 72.40 | 1 | desmin |
| 25 | Mesocricetus auratus | 72.40 | 1 | DESM_MESAU Desmin gb |
| 26 | Scyliorhinus stellaris | 72.00 | 1 | desmin |
| 27 | Acipenser baerii | 71.60 | 2 | desmin |
| 28 | Takifugu rubripes | 71.20 | 1 | TPA: desmin |
| 29 | Danio rerio | 70.50 | 18 | desmin |
| 30 | Oncorhynchus mykiss | 70.50 | 2 | VIME_ONCMY Vimentin emb |
| 31 | Tetraodon nigroviridis | 70.10 | 6 | unnamed protein product |
| 32 | Protopterus aethiopicus | 68.60 | 2 | type III desmin |
| 33 | Oreochromis mossambicus | 66.20 | 1 | glial fibrillary acidic protein |
| 34 | Equus caballus | 63.90 | 1 | glial fibrillary acidic protein |
| 35 | Capreolus capreolus | 63.90 | 1 | glial fibrillary acidic protein |
| 36 | Lampetra fluviatilis | 63.90 | 1 | intermediate filament protein |
| 37 | Dama dama | 63.90 | 1 | glial fibrillary acidic protein |
| 38 | Pongo pygmaeus | 63.50 | 5 | hypothetical protein |
| 39 | Petromyzon marinus | 60.50 | 1 | neurofilament subunit NF-L |
Top-ranked sequences
| organism | matching |
|---|---|
| Homo sapiens | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |||||||||||#|||||||||||||||||||||||||||||| Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |
| Mus musculus | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |||||||||||#|||||||||||||||||||||||||||||| Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |
| N/A | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |||||||||||#|||||||||||||||||||||||||||||| Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |
| Rattus norvegicus | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |||||||||||#|||||||||||||||||||||||||||||| Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |
| Macaca mulatta | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |||||||||||#|||||||||||||||||||||||||||||| Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |
| Pan troglodytes | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |||||||||||#|||||||||||||||||||||||||||||| Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |
| Macaca fascicularis | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |||||||||||#|||||||||||||||||||||||||||||| Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |
| synthetic construct | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |||||||||||#|||||||||||||||||||||||||||||| Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |
| Cricetulus griseus | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |||||||||||#|||||||||||||||||||||||||||||| Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |
| Pan troglodytes verus | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |||||||||||#|||||||||||||||||||||||||||||| Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |
| Canis familiaris | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||||+||||||#|||||||||||||||||||||||||||||| Sbjct 254 VQIDMDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |
| Bos taurus | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||||+||||||#|||||||||||||||||||||||||||||| Sbjct 254 VQIDMDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |
| Bubalus bubalis | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||||+||||||#|||||||||||||||||||||||||||||| Sbjct 59 VQIDMDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 99 |
| Sus scrofa domestica | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||||+||||||#|||||||||||||||||||||||||||||| Sbjct 124 VQIDMDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 164 |
| Monodelphis domestica | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 +||||||+|||#|||||||||||||||||||||||||||||| Sbjct 255 IQIDVDVAKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 295 |
| Gallus gallus | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 +|||+||||||#|||||||||||||||||||||||||||||| Sbjct 248 IQIDMDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 288 |
| Ovis aries | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||||+|||+||#|||||||||||||||||||||||||||||| Sbjct 254 VQIDMDVSQPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 |
| Daboia russellii | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 +|||+||+|||#|||||||||||||||| ||||||||||||| Sbjct 249 IQIDMDVAKPD#LTAALRDVRQQYESVATKNLQEAEEWYKSK 289 |
| Xenopus laevis | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 +|+|+||||||#|||||||||||||+|||||| +|||||||| Sbjct 248 IQVDMDVSKPD#LTAALRDVRQQYENVAAKNLSDAEEWYKSK 288 |
| Bos grunniens | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWY 291 ||||+||||||#||||||||||||||||||||||||||| Sbjct 67 VQIDMDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWY 104 |
| Xenopus tropicalis | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 +|+|+||||||#|||||||||||||+|||||| +||+||||| Sbjct 273 IQVDMDVSKPD#LTAALRDVRQQYENVAAKNLSDAEDWYKSK 313 |
| Cyprinus carpio | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||||++|+|||#|||||+|||||||++|++||||+||||||| Sbjct 245 VQIDMEVAKPD#LTAALKDVRQQYETLASRNLQESEEWYKSK 285 |
| Sus scrofa | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||+++|+||||#|||||||+| |||++||||+ ||||||||| Sbjct 251 VQVEMDMSKPD#LTAALRDIRAQYETIAAKNISEAEEWYKSK 291 |
| Canis lupus familiaris | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||+++|+||||#|||||||+| |||++||||+ ||||||||| Sbjct 258 VQVEMDMSKPD#LTAALRDIRAQYETIAAKNISEAEEWYKSK 298 |
| Mesocricetus auratus | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||+++|+||||#|||||||+| |||++||||+ ||||||||| Sbjct 258 VQVEMDMSKPD#LTAALRDIRAQYETIAAKNISEAEEWYKSK 298 |
| Scyliorhinus stellaris | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||+++|+||||#|||||||+| ||| ++|||+|||||||||| Sbjct 243 VQVEMDLSKPD#LTAALRDIRAQYECISAKNVQEAEEWYKSK 283 |
| Acipenser baerii | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||+++|+||||#| |||+|+| |||++||||+|||||||||| Sbjct 248 VQVEMDMSKPD#LAAALKDIRSQYENIAAKNIQEAEEWYKSK 288 |
| Takifugu rubripes | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||| +|+||||#|||||||+| ||| +||||+ ||||||||| Sbjct 234 VQIQMDMSKPD#LTAALRDIRVQYEGIAAKNISEAEEWYKSK 274 |
| Danio rerio | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||| +|+||||#|||||||+| |||++||||+ |||+||||| Sbjct 253 VQIQMDMSKPD#LTAALRDIRLQYEAIAAKNISEAEDWYKSK 293 |
| Oncorhynchus mykiss | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||||+||+|||#||||||||| |||++|++|||++|+||||| Sbjct 250 VQIDMDVAKPD#LTAALRDVRVQYETLASRNLQDSEDWYKSK 290 |
| Tetraodon nigroviridis | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 ||+|++++|||#||||||||| |||++|+||+||+||||||| Sbjct 228 VQVDMEMAKPD#LTAALRDVRLQYENLASKNIQESEEWYKSK 268 |
| Protopterus aethiopicus | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 + +++|+||||#|||||||+| ||||+||||+ +||||||+| Sbjct 243 IHVEMDMSKPD#LTAALRDIRAQYESIAAKNIADAEEWYKTK 283 |
| Oreochromis mossambicus | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKS 293 | +|+||||||#|||||||+| |||++|+ |+|| ||||+| Sbjct 140 VHVDLDVSKPD#LTAALRDIRVQYENMASSNMQETEEWYRS 179 |
| Equus caballus | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 | +++||+|||#||||||++| |||++|+ |+ ||||||+|| Sbjct 64 VHVELDVAKPD#LTAALREIRTQYEAMASSNMHEAEEWYRSK 104 |
| Capreolus capreolus | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 | +++||+|||#||||||++| |||++|+ |+ ||||||+|| Sbjct 64 VHVEMDVAKPD#LTAALREIRTQYEAMASSNMHEAEEWYRSK 104 |
| Lampetra fluviatilis | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 | ++|| |||#| ||||||| ||||+|+ |||||| |+||| Sbjct 151 VTVEVDTVKPD#LAAALRDVRAQYESIASSNLQEAETWFKSK 191 |
| Dama dama | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 | +++||+|||#||||||++| |||++|+ |+ ||||||+|| Sbjct 64 VHVEMDVAKPD#LTAALREIRTQYEAMASSNMHEAEEWYRSK 104 |
| Pongo pygmaeus | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 + +++||+|||#|+|||+|+| ||| +||||+| ||||+||+ Sbjct 243 ISVEMDVTKPD#LSAALKDIRAQYEKLAAKNMQNAEEWFKSR 283 |
| Petromyzon marinus | Query 254 VQIDVDVSKPD#LTAALRDVRQQYESVAAKNLQEAEEWYKSK 294 + +++ |++||#||+||||+| ||| +||||+| ||||+|+| Sbjct 249 ITVEMSVARPD#LTSALRDIRAQYEVLAAKNMQSAEEWFKTK 289 |
* References
[PubMed ID: 17574350] Sakate R, Suto Y, Imanishi T, Tanoue T, Hida M, Hayasaka I, Kusuda J, Gojobori T, Hashimoto K, Hirai M, Mapping of chimpanzee full-length cDNAs onto the human genome unveils large potential divergence of the transcriptome. Gene. 2007 Sep 1;399(1):1-10. Epub 2007 Apr 20.