* Structure Information
1. Primary Information
Length: 699 aa
Average Mass: 79.875 kDa
Monoisotopic Mass: 79.825 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
| --- cleavage 18 --- |
| --- cleavage 8 --- |
| Peptidase_C2 1. | 44 | 343 | 808.0 | 6.2e-240 |
| Calpain_III 1. | 354 | 509 | 393.6 | 3.4e-115 |
| efhand 1. | 575 | 603 | 20.4 | 0.0074 |
| efhand 2. | 605 | 633 | 19.4 | 0.015 |
| efhand 3. | 670 | 698 | 4.0 | 7.6 |
3. Sequence Information
Fasta Sequence: XSB2663.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Known Structures in PDB:
1KFU (X-ray; 250 A; L=2-700), 1KFX (X-ray; 315 A; L=2-700), 2NQA (X-ray; 220 A; A/B=26-346)
* Cleavage Information
2 [sites]
Cleavage sites (±10aa)
[Site 1] KDREAAEGLG18-SHERAIKYLN
Gly18 
Ser
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Lys9 | Asp10 | Arg11 | Glu12 | Ala13 | Ala14 | Glu15 | Gly16 | Leu17 | Gly18 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Ser19 | His20 | Glu21 | Arg22 | Ala23 | Ile24 | Lys25 | Tyr26 | Leu27 | Asn28 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
AGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNECLEAGTLFQDP
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Homo sapiens |
99.00 |
25 |
calpain 2, large subunit |
| 2 |
N/A |
99.00 |
14 |
L |
| 3 |
catalytic |
99.00 |
1 |
Calpain 2, large |
| 4 |
Macaca mulatta |
97.40 |
3 |
PREDICTED: calpain 2, large subunit |
| 5 |
Mus musculus |
93.60 |
19 |
unnamed protein product |
| 6 |
Rattus norvegicus |
93.60 |
9 |
calpain 2 |
| 7 |
Bos taurus |
92.40 |
7 |
Unknown (protein for MGC:138930) |
| 8 |
Monodelphis domestica |
78.20 |
2 |
PREDICTED: similar to calpain 2 |
| 9 |
Gallus gallus |
74.30 |
6 |
calpain |
| 10 |
Coturnix coturnix |
70.10 |
1 |
quail calpain |
| 11 |
Xenopus laevis |
69.70 |
10 |
hypothetical protein LOC399075 |
| 12 |
Xenopus tropicalis |
69.70 |
5 |
calpain 2, (m/II) large subunit |
| 13 |
Canis familiaris |
67.40 |
4 |
PREDICTED: similar to calpain 8 |
| 14 |
Danio rerio |
64.30 |
27 |
hypothetical protein LOC550505 |
| 15 |
Stizostedion vitreum vitreum |
61.20 |
1 |
putative calpain-like protein |
| 16 |
Tetraodon nigroviridis |
60.10 |
7 |
unnamed protein product |
| 17 |
Oncorhynchus mykiss |
60.10 |
3 |
m-calpain |
| 18 |
Pan troglodytes |
59.70 |
6 |
PREDICTED: hypothetical protein isoform 10 |
| 19 |
Pongo pygmaeus |
59.70 |
1 |
CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1 |
| 20 |
synthetic construct |
59.70 |
1 |
Homo sapiens calpain 1, (mu/I) large subunit |
| 21 |
Sus scrofa |
57.00 |
3 |
micromolar calcium-activated neutral protease 1 is |
| 22 |
Macaca fascicularis |
42.70 |
1 |
unnamed protein product |
| 23 |
Aedes aegypti |
35.00 |
2 |
calpain, putative |
| 24 |
Oryctolagus cuniculus |
34.30 |
1 |
calpain 3, (p94) |
| 25 |
Bos grunniens |
33.90 |
1 |
micromolar calcium-activated neutral protease 1 la |
| 26 |
Drosophila melanogaster |
33.10 |
6 |
CAA55297.1 |
| 27 |
Anopheles gambiae str. PEST |
32.70 |
1 |
ENSANGP00000028181 |
Top-ranked sequences
| organism | matching |
|---|
| Homo sapiens |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
|
| N/A |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
|
| catalytic |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
|
| Macaca mulatta |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
||||||| ||||||||||#||||||||||||||||||||||||||||||
Sbjct 2 AGIAAKLVKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49
|
| Mus musculus |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
|||| |||||||||||||#|||||||||||||| ||||||||| |||||
Sbjct 2 AGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49
|
| Rattus norvegicus |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
|||| |||||||||||||#|||||||||||||| ||||||||| |||||
Sbjct 2 AGIAMKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49
|
| Bos taurus |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
||||||||||||||||||#|||||+||||||| |||+|||||| |||||
Sbjct 2 AGIAAKLAKDREAAEGLG#SHERAVKYLNQDYAALRDECLEAGALFQDP 49
|
| Monodelphis domestica |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
+|||||+ +||||| |||#+||+|+|||||||| | +|||| | |||||
Sbjct 2 SGIAAKVVRDREAAAGLG#THEQAVKYLNQDYETLLSECLEEGKLFQDP 49
|
| Gallus gallus |
Query 2 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
||||+| +|| |||+|# | |+||||||||||+ ||+|+||||+||
Sbjct 6 GIAARLQRDRLRAEGVG#EHNNAVKYLNQDYEALKQECIESGTLFRDP 52
|
| Coturnix coturnix |
Query 2 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
||||+| +|| |||+|# | |+||||||||||+ |+|+| ||+||
Sbjct 6 GIAARLQRDRLRAEGVG#EHNNAVKYLNQDYEALKQACIESGALFRDP 52
|
| Xenopus laevis |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
+|+| +|||+| | | |#++|+|+ |||||+||||||||++|+||+||
Sbjct 2 SGVADRLAKERARASGFG#TNEKALPYLNQDFEALRNECLQSGSLFKDP 49
|
| Xenopus tropicalis |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 47
+|+| +|||+| | |||#++|+|+ ||+|||||||||||++||||+|
Sbjct 2 SGVADRLAKERALASGLG#TNEKALPYLSQDYEALRNECLQSGTLFKD 48
|
| Canis familiaris |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
+ +||++|| + || |||#||++|+||| ||+| || +||+|| ||+||
Sbjct 95 SALAARIAKQQAAAGGLG#SHQKAVKYLGQDFEKLRQQCLDAGVLFKDP 142
|
| Danio rerio |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 47
+||| || |+| | |+|#++ +| |+||||||||+ ||||+| ||||
Sbjct 2 SGIATKLQKNRARAAGIG#TNAQAAKFLNQDYEALKRECLESGRLFQD 48
|
| Stizostedion vitreum vitreum |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 47
+|||| | + || +|+|#| +|+||||||||||| |||+ +||+|
Sbjct 2 SGIAATLQRRREKEQGIG#SCSQAVKYLNQDYEALRRRCLESESLFKD 48
|
| Tetraodon nigroviridis |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 47
+|||||| |+ |+| |#+ +| ||||||+|+|| ||| | ||||
Sbjct 2 SGIAAKLQHHRDRAQGFG#TASQAQKYLNQDFESLRESCLERGELFQD 48
|
| Oncorhynchus mykiss |
Query 1 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
+|+|+ ||| | | | |#++ |+||||||+|||| +|| | || ||
Sbjct 2 SGMASNLAKKRALAAGFG#TNANAVKYLNQDFEALRAQCLNRGGLFSDP 49
|
| Pan troglodytes |
Query 2 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 47
|++|++ | | |||# || ||||| |||| || ||++||||+|
Sbjct 13 GVSAQVQKQRARELGLG#RHENAIKYLGQDYEQLRVRCLQSGTLFRD 58
|
| Pongo pygmaeus |
Query 2 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 47
|++|++ | | |||# || ||||| |||| || ||++||||+|
Sbjct 13 GVSAQVQKQRARELGLG#RHENAIKYLGQDYEQLRVRCLQSGTLFRD 58
|
| synthetic construct |
Query 2 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 47
|++|++ | | |||# || ||||| |||| || ||++||||+|
Sbjct 13 GVSAQVQKQRARELGLG#RHENAIKYLGQDYEQLRVRCLQSGTLFRD 58
|
| Sus scrofa |
Query 2 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 47
|++|++ | | |||# || ||||| |||| || ||++|+||+|
Sbjct 13 GVSAQVQKLRAKELGLG#RHENAIKYLGQDYEQLRAHCLQSGSLFRD 58
|
| Macaca fascicularis |
Query 2 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 48
|+ | + | | |+|# | | + || +| || || | ||+||
Sbjct 36 GMVAHINYSRLKASGVG#QHHNAHNFGNQSFEELRAVCLRKGELFEDP 82
|
| Aedes aegypti |
Query 29 QDYEALRNECLEAGTLFQDP 48
||| ||++|||+|+||+||
Sbjct 115 QDYYVLRSQCLESGSLFEDP 134
|
| Oryctolagus cuniculus |
Query 6 KLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 47
++ ++|+ | |#+ + ||| ||+ || || ||+|
Sbjct 12 RVIRERDRRNGEG#TVTQPIKYEGQDFVVLRQRCLAQKCLFED 53
|
| Bos grunniens |
Query 26 YLNQDYEALRNECLEAGTLFQD 47
|| |||| || ||+ | ||+|
Sbjct 1 YLGQDYEQLRVHCLQRGALFRD 22
|
| Drosophila melanogaster |
Query 29 QDYEALRNECLEAGTLFQDP 48
|||| + | || +|+||+||
Sbjct 50 QDYETILNSCLASGSLFEDP 69
|
| Anopheles gambiae str. PEST |
Query 29 QDYEALRNECLEAGTLFQDP 48
+|++|||+ || ||||+||
Sbjct 46 KDFQALRDRCLAEGTLFEDP 65
|
[Site 2] AGIAAKLA8-KDREAAEGLG
Ala8 Lys
|
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| - | - | Ala1 | Gly2 | Ile3 | Ala4 | Ala5 | Lys6 | Leu7 | Ala8 |
|
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Lys9 | Asp10 | Arg11 | Glu12 | Ala13 | Ala14 | Glu15 | Gly16 | Leu17 | Gly18 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
AGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNEC
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Homo sapiens |
79.00 |
20 |
AF261089_1 calpain large polypeptide L2 |
| 2 |
N/A |
79.00 |
12 |
L |
| 3 |
catalytic |
79.00 |
1 |
Calpain 2, large |
| 4 |
Macaca mulatta |
77.40 |
3 |
PREDICTED: calpain 2, large subunit |
| 5 |
Mus musculus |
75.50 |
15 |
calpain 2 |
| 6 |
Rattus norvegicus |
75.50 |
5 |
calpain 2 |
| 7 |
Bos taurus |
74.30 |
6 |
Unknown (protein for MGC:138930) |
| 8 |
Monodelphis domestica |
62.40 |
2 |
PREDICTED: similar to calpain 2 |
| 9 |
Gallus gallus |
57.80 |
5 |
calpain 1, (mu/I) large subunit |
| 10 |
Xenopus tropicalis |
56.20 |
5 |
calpain 2, (m/II) large subunit |
| 11 |
Coturnix coturnix |
55.50 |
1 |
quail calpain |
| 12 |
Xenopus laevis |
55.10 |
9 |
hypothetical protein LOC399075 |
| 13 |
Canis familiaris |
52.00 |
4 |
PREDICTED: similar to calpain 8 |
| 14 |
Stizostedion vitreum vitreum |
51.20 |
1 |
putative calpain-like protein |
| 15 |
Danio rerio |
50.40 |
23 |
hypothetical protein LOC550505 |
| 16 |
Oncorhynchus mykiss |
49.30 |
3 |
calpain 2 catalytic subunit |
| 17 |
Tetraodon nigroviridis |
47.00 |
6 |
unnamed protein product |
| 18 |
Pan troglodytes |
46.20 |
5 |
PREDICTED: hypothetical protein isoform 10 |
| 19 |
Pongo pygmaeus |
46.20 |
1 |
CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1 |
| 20 |
synthetic construct |
46.20 |
1 |
Homo sapiens calpain 1, (mu/I) large subunit |
| 21 |
Sus scrofa |
45.10 |
2 |
micromolar calcium-activated neutral protease 1 is |
Top-ranked sequences
| organism | matching |
|---|
| Homo sapiens |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
||||||||#||||||||||||||||||||||||||||||
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
|
| N/A |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
||||||||#||||||||||||||||||||||||||||||
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
|
| catalytic |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
||||||||#||||||||||||||||||||||||||||||
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
|
| Macaca mulatta |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
||||||| #||||||||||||||||||||||||||||||
Sbjct 2 AGIAAKLV#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39
|
| Mus musculus |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
|||| |||#|||||||||||||||||||||||| |||||
Sbjct 2 AGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|
| Rattus norvegicus |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
|||| |||#|||||||||||||||||||||||| |||||
Sbjct 2 AGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39
|
| Bos taurus |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
||||||||#|||||||||||||||+||||||| |||+||
Sbjct 2 AGIAAKLA#KDREAAEGLGSHERAVKYLNQDYAALRDEC 39
|
| Monodelphis domestica |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
+|||||+ #+||||| |||+||+|+|||||||| | +||
Sbjct 2 SGIAAKVV#RDREAAAGLGTHEQAVKYLNQDYETLLSEC 39
|
| Gallus gallus |
Query 2 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
||||+| #+|| |||+| | |+||||||||||+ ||
Sbjct 6 GIAARLQ#RDRLRAEGVGEHNNAVKYLNQDYEALKQEC 42
|
| Xenopus tropicalis |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
+|+| +||#|+| | |||++|+|+ ||+||||||||||
Sbjct 2 SGVADRLA#KERALASGLGTNEKALPYLSQDYEALRNEC 39
|
| Coturnix coturnix |
Query 2 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
||||+| #+|| |||+| | |+||||||||||+ |
Sbjct 6 GIAARLQ#RDRLRAEGVGEHNNAVKYLNQDYEALKQAC 42
|
| Xenopus laevis |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
+|+| +||#|+| | | |++|+|+ |||||+|||||||
Sbjct 2 SGVADRLA#KERARASGFGTNEKALPYLNQDFEALRNEC 39
|
| Canis familiaris |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
+ +||++|#| + || |||||++|+||| ||+| || +|
Sbjct 95 SALAARIA#KQQAAAGGLGSHQKAVKYLGQDFEKLRQQC 132
|
| Stizostedion vitreum vitreum |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
+|||| | #+ || +|+|| +|+||||||||||| |
Sbjct 2 SGIAATLQ#RRREKEQGIGSCSQAVKYLNQDYEALRRRC 39
|
| Danio rerio |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
+||| || #|+| | |+|++ +| |+||||||||+ ||
Sbjct 2 SGIATKLQ#KNRARAAGIGTNAQAAKFLNQDYEALKREC 39
|
| Oncorhynchus mykiss |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
||+|+ ||#| | | | |++ | ||||||+|+||+||
Sbjct 2 AGVASTLA#KKRALAAGFGTNANASKYLNQDFESLRSEC 39
|
| Tetraodon nigroviridis |
Query 1 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
+|||||| # |+ |+| |+ +| ||||||+|+|| |
Sbjct 2 SGIAAKLQ#HHRDRAQGFGTASQAQKYLNQDFESLRESC 39
|
| Pan troglodytes |
Query 2 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
|++|++ #| | ||| || ||||| |||| || |
Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49
|
| Pongo pygmaeus |
Query 2 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
|++|++ #| | ||| || ||||| |||| || |
Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49
|
| synthetic construct |
Query 2 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
|++|++ #| | ||| || ||||| |||| || |
Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49
|
| Sus scrofa |
Query 2 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 38
|++|++ #| | ||| || ||||| |||| || |
Sbjct 13 GVSAQVQ#KLRAKELGLGRHENAIKYLGQDYEQLRAHC 49
|
* References
[PubMed ID: 10666632] Masumoto H, Nakagawa K, Irie S, Sorimachi H, Suzuki K, Bourenkov GP, Bartunik H, Fernandez-Catalan C, Bode W, Strobl S, Crystallization and preliminary X-ray analysis of recombinant full-length human m-calpain. Acta Crystallogr D Biol Crystallogr. 2000 Jan;56(Pt 1):73-5.
» More (3) « Hide Below
[PubMed ID: 10639123] ... Strobl S, Fernandez-Catalan C, Braun M, Huber R, Masumoto H, Nakagawa K, Irie A, Sorimachi H, Bourenkow G, Bartunik H, Suzuki K, Bode W, The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium. Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):588-92.
[PubMed ID: 11517928] ... Reverter D, Strobl S, Fernandez-Catalan C, Sorimachi H, Suzuki K, Bode W, Structural basis for possible calcium-induced activation mechanisms of calpains. Biol Chem. 2001 May;382(5):753-66.
[PubMed ID: 11673052] ... Reverter D, Sorimachi H, Bode W, The structure of calcium-free human m-calpain: implications for calcium activation and function. Trends Cardiovasc Med. 2001 Aug;11(6):222-9.
