CaMPDB :: Substrate

XSB2853 : Calpain-1 catalytic subunit

This entry is computationally expanded from SB0020

* Basic Information

Organism	Sus scrofa (pig)
Protein Names	Calpain-1 large subunit; Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; muCANP; Micromolar-calpain; 3.4.22.52
Gene Names	CAPN1
Gene Locus	Not available
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
P35750	N/A	N/A	CAN1_PIG	N/A	N/A	N/A	ssc:

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 714 aa

Average Mass: 81.739 kDa

Monoisotopic Mass: 81.687 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
--- cleavage 15 ---
Peptidase_C2 1.	55	354	799.5	2.2e-237
Calpain_III 1.	365	522	372.4	8.1e-109
efhand 1.	589	617	16.6	0.11
efhand 2.	619	647	18.5	0.028
efhand 3.	684	711	6.2	4.5

3. Sequence Information

Fasta Sequence: XSB2853.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites]

Cleavage sites (±10aa)

[Site 1] ITPVYCTGVS¹⁵-AQVQKLRAKE

Ser¹⁵ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ile⁶	Thr⁷	Pro⁸	Val⁹	Tyr¹⁰	Cys¹¹	Thr¹²	Gly¹³	Val¹⁴	Ser¹⁵

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala¹⁶	Gln¹⁷	Val¹⁸	Gln¹⁹	Lys²⁰	Leu²¹	Arg²²	Ala²³	Lys²⁴	Glu²⁵

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAEEVITPVYCTGVSAQVQKLRAKELGLGRHENAIKYLGQDYEQL

Summary

#	organism	max score	hits	top seq
1	N/A	93.20	11	C
2	Sus scrofa	93.20	4	micromolar calcium-activated neutral protease 1 is
3	Macaca mulatta	90.50	3	PREDICTED: calpain 1, large subunit isoform 4
4	Bos taurus	89.00	6	micromolar calcium activated neutral protease 1
5	Homo sapiens	88.20	17	CAPN1 protein
6	Pan troglodytes	88.20	5	PREDICTED: hypothetical protein isoform 6
7	Canis familiaris	88.20	3	PREDICTED: similar to Calpain-1 catalytic subunit
8	synthetic construct	88.20	1	Homo sapiens calpain 1, (mu/I) large subunit
9	Pongo pygmaeus	88.20	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
10	Rattus norvegicus	85.50	5	calpain 1
11	Mus musculus	83.60	15	calpain I large subunit
12	Xenopus laevis	71.60	7	MGC81785 protein
13	Xenopus tropicalis	70.90	3	CAPN1 protein
14	Gallus gallus	68.60	5	mu-calpain large subunit
15	Danio rerio	42.70	16	calpain 1
16	Coturnix coturnix	42.00	1	quail calpain
17	Monodelphis domestica	40.80	1	PREDICTED: similar to calpain 2
18	catalytic	40.40	1	Calpain 2, large
19	Stizostedion vitreum vitreum	39.70	1	putative calpain-like protein
20	Oncorhynchus mykiss	36.60	3	calpain 1 catalytic subunit
21	Tetraodon nigroviridis	34.70	1	unnamed protein product

Top-ranked sequences

organism	matching
N/A	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45
Sus scrofa	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45
Macaca mulatta	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAEEIITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45
Bos taurus	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45
Homo sapiens	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|+\|\|+\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MSEEIITPVYCTGVS#AQVQKQRARELGLGRHENAIKYLGQDYEQL 45
Pan troglodytes	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|+\|\|+\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MSEEIITPVYCTGVS#AQVQKQRARELGLGRHENAIKYLGQDYEQL 45
Canis familiaris	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|+\|\|\| Sbjct 1 MAEEIITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYMGQDFEQL 45
synthetic construct	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|+\|\|+\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MSEEIITPVYCTGVS#AQVQKQRARELGLGRHENAIKYLGQDYEQL 45
Pongo pygmaeus	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|+\|\|+\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MSEEIITPVYCTGVS#AQVQKQRARELGLGRHENAIKYLGQDYEQL 45
Rattus norvegicus	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\|+\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 1 MAEELITPVYCTGVS#AQVQKQRDKELGLGRHENAIKYLGQDYENL 45
Mus musculus	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \| \|\|+\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 1 MTEELITPVYCTGVS#AQVQKKRDKELGLGRHENAIKYLGQDYETL 45
Xenopus laevis	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\|\|\| \|\|\| \|\|+\|#\|\|+\|\| \| \|\|\|+\|+\|\|\|\|+\|+\|\|\|\|\|+++ Sbjct 1 MAEEVIVPVYATGIS#AQLQKQRDHELGVGKHENAVKFLGQDYDRI 45
Xenopus tropicalis	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\|\|\| \|\|\| +\|+\|#\|\|+\|\| \| +\|\|\|+\|+\|\|\|\|+\|+\|\|\|\|\|+++ Sbjct 1 MAEEVIVPVYASGIS#AQLQKQRDQELGVGKHENAVKFLGQDYDRI 45
Gallus gallus	Query 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\| + \|\|+\|\|\|\|\|#\|\|\|\|+ \|\|\| \|\|\|\|+\|+\|\|+++ \|\|\|\| \| Sbjct 1 MAEEPVVPVFCTGVS#AQVQRQRAKALGLGQHQNAVRFRGQDYAAL 45
Danio rerio	Query 8 PVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|+ \|\|++#\|+++ ++ \|\|\|++ \|\|+\|+\|\|\|\|\|\| \| Sbjct 3 PICATGMA#ARLRSQWDRDAGLGQNHNAVKFLGQDYETL 40
Coturnix coturnix	Query 13 GVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|++#\|++\|+ \| + \|+\| \| \|\|+\|\|\| \|\|\|\| \| Sbjct 6 GIA#ARLQRDRLRAEGVGEHNNAVKYLNQDYEAL 38
Monodelphis domestica	Query 12 TGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 +\|++#\|+\| + \| \|\|\| \|\| \|+\|\|\| \|\|\|\| \| Sbjct 2 SGIA#AKVVRDREAAAGLGTHEQAVKYLNQDYETL 35
catalytic	Query 13 GVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|++#\|++ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \| Sbjct 32 GIA#AKLAKDREAAEGLGSHERAIKYLNQDYEAL 64
Stizostedion vitreum vitreum	Query 12 TGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 +\|++#\| +\|+ \| \|\| \|+\| \|+\|\|\| \|\|\|\| \| Sbjct 2 SGIA#ATLQRRREKEQGIGSCSQAVKYLNQDYEAL 35
Oncorhynchus mykiss	Query 9 VYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 \|\| +\|++#\|+++ ++ \|\|\|++ \|\|+\|+ \|\|\|++ \| Sbjct 4 VYASGMA#AKLRSQWDRDEGLGQNHNAVKFQGQDFKSL 40
Tetraodon nigroviridis	Query 10 YCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45 + \|\|++# +++ ++ \|\|\|++ \|+\|+\|\|\|\|\|\| \| Sbjct 7 HATGMA#YKLRSQWDRDEGLGQNHKAVKFLGQDYELL 42

* References

[PubMed ID: 11219468] Smith TP, Simmen FA, Zhao G, Vallet JL, Rapid communication: Nucleotide sequences of two isoforms of porcine micromolar calcium-activated neutral protease 1 cDNA. J Anim Sci. 2001 Feb;79(2):552-3.

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