CaMPDB :: Substrate

XSB2977 : phospholipase C, beta 1 isoform 2 [Mus musculus]

This entry is computationally expanded from SB0008

* Basic Information

Organism	Mus musculus (house mouse)
Protein Names	Phospholipase C, beta 1; Phospholipase C, beta 1, isoform CRA_a; phospholipase C; beta 1 isoform 2; phospholipase C-beta-1a; phosphoinositide phospholipase C
Gene Names	Plcb1; RP23-418D21.3-003, mCG_16884; phospholipase C, beta 1
Gene Locus	2 76.7 cM; chromosome 2
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_062651	NM_019677	18795	A2AW58_MOUSE	N/A	N/A	N/A	mmu:18795

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 1173 aa

Average Mass: 133.327 kDa

Monoisotopic Mass: 133.243 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
Chitin_synth_1 1.	92	181	-112.3	0.67
efhand_like 1.	224	315	164.0	4.4e-46
PI-PLC-X 1.	318	468	327.6	2.6e-95
PI-PLC-Y 1.	539	656	297.6	2.6e-86
C2 1.	678	761	59.0	1.7e-14
--- cleavage 880 ---
DUF1154 1.	903	946	6.1	0.0037
PLC-beta_C 1.	997	1166	310.5	3.4e-90

3. Sequence Information

Fasta Sequence: XSB2977.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites]

Cleavage sites (±10aa)

[Site 1] APKPPSQAPH⁸⁸⁰-SQPAPGSVKA

His⁸⁸⁰ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ala⁸⁷¹	Pro⁸⁷²	Lys⁸⁷³	Pro⁸⁷⁴	Pro⁸⁷⁵	Ser⁸⁷⁶	Gln⁸⁷⁷	Ala⁸⁷⁸	Pro⁸⁷⁹	His⁸⁸⁰

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser⁸⁸¹	Gln⁸⁸²	Pro⁸⁸³	Ala⁸⁸⁴	Pro⁸⁸⁵	Gly⁸⁸⁶	Ser⁸⁸⁷	Val⁸⁸⁸	Lys⁸⁸⁹	Ala⁸⁹⁰

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
APSETRTTPAENGVNHTASLAPKPPSQAPHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTI

Summary

#	organism	max score	hits	top seq
1	Mus musculus	83.60	7	phospholipase C, beta 1
2	synthetic construct	83.60	1	phospholipase C-beta1
3	N/A	82.40	2	A
4	Rattus norvegicus	82.40	1	phospholipase C, beta 1
5	Homo sapiens	79.00	7	phosphoinositide-specific phospholipase C beta 1 i
6	Pan troglodytes	79.00	1	PREDICTED: phosphoinositide-specific phospholipase
7	Macaca mulatta	78.60	1	PREDICTED: similar to phosphoinositide-specific ph
8	Bos taurus	77.80	1	phosphoinositide-specific phospholipase C beta 1
9	Canis familiaris	75.50	1	PREDICTED: similar to phosphoinositide-specific ph
10	Gallus gallus	73.20	1	PREDICTED: similar to KIAA0581 protein
11	Monodelphis domestica	71.60	1	PREDICTED: similar to phosphoinositide-specific ph

Top-ranked sequences

organism	matching
Mus musculus	Query 851 APSETRTTPAENGVNHTASLXXXXXXXXXX#XXXXXGSVKAPAKTEDLIQSVLTEVEAQTI 910 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| # \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 851 APSETRTTPAENGVNHTASLAPKPPSQAPH#SQPAPGSVKAPAKTEDLIQSVLTEVEAQTI 910
synthetic construct	Query 851 APSETRTTPAENGVNHTASLXXXXXXXXXX#XXXXXGSVKAPAKTEDLIQSVLTEVEAQTI 910 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| # \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 851 APSETRTTPAENGVNHTASLAPKPPSQAPH#SQPAPGSVKAPAKTEDLIQSVLTEVEAQTI 910
N/A	Query 851 APSETRTTPAENGVNHTASLXXXXXXXXXX#XXXXXGSVKAPAKTEDLIQSVLTEVEAQTI 910 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\| # \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 851 APSETRTTPAENGVNHTATLAPKPPSQAPH#SQPAPGSVKAPAKTEDLIQSVLTEVEAQTI 910
Rattus norvegicus	Query 851 APSETRTTPAENGVNHTASLXXXXXXXXXX#XXXXXGSVKAPAKTEDLIQSVLTEVEAQTI 910 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\| # \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 851 APSETRTTPAENGVNHTATLAPKPPSQAPH#SQPAPGSVKAPAKTEDLIQSVLTEVEAQTI 910
Homo sapiens	Query 851 APSETRTTPAENGVNHTASLXXXXXXXXXX#XXXXXGSVKAPAKTEDLIQSVLTEVEAQTI 910 \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| +\| # \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 851 APSEARTTPAENGVNHTTTLTPKPPSQALH#SQPAPGSVKAPAKTEDLIQSVLTEVEAQTI 910
Pan troglodytes	Query 851 APSETRTTPAENGVNHTASLXXXXXXXXXX#XXXXXGSVKAPAKTEDLIQSVLTEVEAQTI 910 \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| +\| # \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 906 APSEARTTPAENGVNHTTTLTPKPPSQALH#SQPAPGSVKAPAKTEDLIQSVLTEVEAQTI 965
Macaca mulatta	Query 852 PSETRTTPAENGVNHTASLXXXXXXXXXX#XXXXXGSVKAPAKTEDLIQSVLTEVEAQTI 910 \|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\| # \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 852 PSEVRTTPAENGVNHTTSLTPKPPSQALH#SQPAPGSVKAPAKTEDLIQSVLTEVEAQTI 910
Bos taurus	Query 851 APSETRTTPAENGVNHTASLXXXXXXXXXX#XXXXXGSVKAPAKTEDLIQSVLTEVEAQTI 910 \|\|\|\| \| \|\|\|\|\|\|\|\|\|\| \|\| # \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 851 APSEARPTPAENGVNHTTSLTPKPPSQALH#SQPAPGSVKAPAKTEDLIQSVLTEVEAQTI 910
Canis familiaris	Query 851 APSETRTTPAENGVNHTASLXXXXXXXXXX#XXXXXGSVKAPAKTEDLIQSVLTEVEAQTI 910 \|\|\|\| \| \|\|\|\|\|\|\|\|\| \|\| # \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 845 APSEAGTNPAENGVNHTTSLAPKPPSQALH#SQPAPGSVKAPAKTEDLIQSVLTEVEAQTI 904
Gallus gallus	Query 851 APSETRTTPAENGVNHTASLXXXXXXXXXX#XXXXXGSVKAPAKTEDLIQSVLTEVEAQTI 910 \| \|\| \| \|\|\|\|\|\|\|\|\|\|\|\|\| # \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1052 ANSEPRPTPAENGVNHTASLTSKPTTQVVH#SQPTPGLVKAPAKTEDLIQSVLTEVEAQTI 1111
Monodelphis domestica	Query 853 SETRTTPAENGVNHTASLXXXXXXXXXX#XXXXXGSVKAPAKTEDLIQSVLTEVEAQTI 910 \|\| \| \|\|\|\|\|\|\|\|\|\|\|\| # \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 854 SEPRPAPAENGVNHTASLAPKPPSQVVH#SQPAPGSVKAPAKTEDLIQSVLTDVEAQTI 911

* References

[PubMed ID: 19385066] O'Carroll SJ, Mitchell MD, Faenza I, Cocco L, Gilmour RS, Nuclear PLCbeta1 is required for 3T3-L1 adipocyte differentiation and regulates expression of the cyclin D3-cdk4 complex. Cell Signal. 2009 Jun;21(6):926-35.

» More (9) « Hide Below

[PubMed ID: 19028838] ... Fiume R, Ramazzotti G, Teti G, Chiarini F, Faenza I, Mazzotti G, Billi AM, Cocco L, Involvement of nuclear PLCbeta1 in lamin B1 phosphorylation and G2/M cell cycle progression. FASEB J. 2009 Mar;23(3):957-66. Epub 2008 Nov 21.

[PubMed ID: 18957514] ... Adjobo-Hermans MJ, Goedhart J, Gadella TW Jr, Regulation of PLCbeta1a membrane anchoring by its substrate phosphatidylinositol (4,5)-bisphosphate. J Cell Sci. 2008 Nov 15;121(Pt 22):3770-7. Epub 2008 Oct 28.

[PubMed ID: 18973591] ... Fukaya M, Uchigashima M, Nomura S, Hasegawa Y, Kikuchi H, Watanabe M, Predominant expression of phospholipase Cbeta1 in telencephalic principal neurons and cerebellar interneurons, and its close association with related signaling molecules in somatodendritic neuronal elements. Eur J Neurosci. 2008 Nov;28(9):1744-59.

[PubMed ID: 18692062] ... Vasilevski O, Grubb DR, Filtz TM, Yang S, McLeod-Dryden TJ, Luo J, Karna D, Chen J, Woodcock EA, Ins(1,4,5)P(3) regulates phospholipase Cbeta1 expression in cardiomyocytes. J Mol Cell Cardiol. 2008 Nov;45(5):679-84. Epub 2008 Jul 23.

[PubMed ID: 8672127] ... Lyu MS, Park DJ, Rhee SG, Kozak CA, Genetic mapping of the human and mouse phospholipase C genes. Mamm Genome. 1996 Jul;7(7):501-4.

[PubMed ID: 8617512] ... Zuberi AR, Nguyen HQ, Auman HJ, Taylor BA, Roopenian DC, A genetic linkage map of mouse chromosome 2 extending from thrombospondin to paired box gene 1, including the H3 minor histocompatibility complex. Genomics. 1996 Apr 1;33(1):75-84.

[PubMed ID: 7642703] ... Walensky LD, Snyder SH, Inositol 1,4,5-trisphosphate receptors selectively localized to the acrosomes of mammalian sperm. J Cell Biol. 1995 Aug;130(4):857-69.

[PubMed ID: 7916325] ... Hess EJ, Collins KA, Copeland NG, Jenkins NA, Wilson MC, Deletion map of the coloboma (Cm) locus on mouse chromosome 2. Genomics. 1994 May 1;21(1):257-61.

[PubMed ID: 2033248] ... Hempel WM, DeFranco AL, Expression of phospholipase C isozymes by murine B lymphocytes. J Immunol. 1991 Jun 1;146(11):3713-20.